ORF » Species Summary » Homo sapiens » LIG1 cDNA ORF clone

LIG1 cDNA ORF clone, Homo sapiens(human)

The following LIG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14319 NM_000234.3
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu14319 XM_006723216.2
Latest version!		 Homo sapiens ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu14238 NM_001289063.2
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14327 NM_001289064.1
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu52262 NM_001320970.2
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu52263 NM_001320971.2
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu88469 XM_024451513.1
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu88469 XM_017026809.1
Latest version!		 Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OHu52263 NM_001320971.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14319 NM_000234.2 Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu52262 NM_001320970.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu14238 NM_001289063.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LIG1 ( NM_000234.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000225.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu14319
    Clone ID Related Accession (Same CDS sequence) NM_000234.3 , XM_006723216.2 , NM_000234.2
    Accession Version NM_000234.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-30
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108318.1. On Nov 23, 2018 this sequence version replaced NM_000234.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108318.1, M36067.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263274.12/ ENSP00000263274.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000225.1
    CDS162..2921
    Translation

    Target ORF information:

    RefSeq Version NM_000234.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000234.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_000234.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000225.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu14319
    Clone ID Related Accession (Same CDS sequence) NM_000234.3 , XM_006723216.2 , NM_000234.2
    Accession Version NM_000234.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108318.1. On Jan 15, 2014 this sequence version replaced NM_000234.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108318.1, SRR1803612.206854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000225.1
    CDS421..3180
    Translation

    Target ORF information:

    RefSeq Version NM_000234.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_000234.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( XM_006723216.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723279.1 Homo sapiens ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu14319
    Clone ID Related Accession (Same CDS sequence) NM_000234.3 , XM_006723216.2 , NM_000234.2
    Accession Version XM_006723216.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2760bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 1 isoform X1
    Comment 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723279.1
    Translation

    Target ORF information:

    RefSeq Version XM_006723216.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase I, DNA, ATP-dependent (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006723216.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGA GGAGACCCCG 
    AAAGAAAGCC TCACAGAGGC TGAAGTGGCC ACAGAGAAGG AAGGAGAAGA CGGGGACCAG 
    CCCACCACGC CTCCCAAGCC CCTAAAGACC TCCAAAGCAG AGACCCCGAC GGAAAGCGTT 
    TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA CTGCAGGAGG AGGAAGAGCA GACCAAGCCT 
    CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC TTCTTCACCC CCCGGAAGCC AGCAGTCAAA 
    AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT CCAGGAAAGG AGGGAGCTGC TGAGGGACCC 
    CTGGATCCAT CTGGTTACAA TCCTGCCAAG AACAACTATC ATCCCGTGGA AGATGCCTGC 
    TGGAAACCGG GCCAGAAGGT TCCTTACCTG GCTGTGGCCC GGACGTTTGA GAAGATCGAG 
    GAGGTGTCTG CTCGGCTCCG GATGGTGGAG ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG 
    GCCCTGTCGC CTCCAGACCT CCTCCCTGTC CTCTACCTCA GCCTCAACCA CCTTGGGCCA 
    CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT GATGGTGTCC TTCTCAAGGC AGTGGCCCAG 
    GCCACAGGTC GGCAGCTGGA GTCCGTCCGG GCTGAGGCAG CCGAGAAAGG CGACGTGGGG 
    CTGGTGGCCG AGAACAGCCG CAGCACCCAG AGGCTCATGC TGCCACCACC TCCGCTCACT 
    GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC ATCGCCAGGC TCACTGGCAG TGCTTCCACA 
    GCCAAGAAGA TAGACATCAT CAAAGGCCTC TTTGTGGCCT GCCGCCACTC AGAAGCCCGG 
    TTCATCGCTA GGTCCCTGAG CGGACGGCTG CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG 
    GCTGCCCTCT CCCAGGCAGT GAGCCTCACG CCCCCGGGCC AAGAATTCCC ACCAGCCATG 
    GTGGATGCTG GGAAGGGCAA GACAGCAGAG GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC 
    ATGATCCTGA AGCAGACGTT CTGCGAGGTT CCCGACCTGG ACCGAATTAT CCCCGTGCTG 
    CTGGAGCACG GCCTGGAACG TCTCCCGGAG CACTGCAAGC TGAGCCCAGG GATTCCCCTG 
    AAACCAATGT TGGCCCATCC CACCCGGGGC ATCAGCGAGG TCCTGAAACG CTTTGAGGAG 
    GCAGCTTTCA CCTGCGAATA CAAATATGAC GGGCAGAGGG CACAGATCCA CGCCCTGGAA 
    GGCGGGGAGG TGAAGATCTT CAGCAGGAAT CAGGAAGACA ACACTGGGAA GTACCCGGAC 
    ATCATCAGCC GCATCCCCAA GATTAAACTC CCATCGGTCA CATCCTTCAT CCTGGACACC 
    GAAGCCGTGG CTTGGGACCG GGAAAAGAAG CAGATCCAGC CATTCCAAGT GCTCACCACC 
    CGCAAACGCA AGGAGGTGGA TGCGTCTGAG ATCCAGGTGC AGGTGTGTTT GTACGCCTTC 
    GACCTCATCT ACCTCAATGG AGAGTCCCTG GTACGTGAGC CCCTTTCCCG GCGCCGGCAG 
    CTGCTCCGGG AGAACTTTGT GGAGACAGAG GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC 
    ACCAAGGACA TCGAGCAGAT CGCCGAGTTC CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG 
    GGGCTGATGG TGAAGACCCT GGATGTTGAT GCCACCTACG AGATCGCCAA GAGATCGCAC 
    AACTGGCTCA AGCTGAAGAA GGACTACCTT GATGGCGTGG GTGACACCCT GGACCTGGTG 
    GTGATCGGCG CCTACCTGGG CCGGGGGAAG CGGGCCGGCC GGTACGGGGG CTTCCTGCTG 
    GCCTCCTACG ACGAGGACAG TGAGGAGCTG CAGGCCATAT GCAAGCTTGG AACTGGCTTC 
    AGTGATGAGG AGCTGGAGGA GCATCACCAG AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA 
    CGCCCTTACG TGCGGATAGA TGGCGCTGTG ATTCCCGACC ACTGGCTGGA CCCCAGCGCT 
    GTGTGGGAGG TGAAGTGCGC TGACCTCTCC CTCTCTCCCA TCTACCCTGC TGCGCGGGGC 
    CTGGTGGATA GTGACAAGGG CATCTCCCTT CGCTTCCCTC GGTTTATTCG AGTCCGTGAA 
    GACAAGCAGC CGGAGCAGGC CACCACCAGT GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA 
    AGTCAGATTC AGAACCAACA AGGCGAGGAC TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001289063.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275992.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu14238
    Clone ID Related Accession (Same CDS sequence) NM_001289063.1 , NM_001289063.2
    Accession Version NM_001289063.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-22
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108318.1 and AK300370.1. On Jan 15, 2014 this sequence version replaced XM_005258935.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300370.1, SRR1803612.252686.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAGGA GACCCCGAAA GAAAGCCTCA CAGAGGCTGA AGTGGCCACA 
    GAGAAGGAAG GAGAAGACGG GGACCAGCCC ACCACGCCTC CCAAGCCCCT AAAGACCTCC 
    AAAGCAGAGA CCCCGACGGA AAGCGTTTCA GAGCCTGAGG TGGCCACGAA GCAGGAACTG 
    CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC CGCAGAGCTC CCAAGACGCT CAGCAGCTTC 
    TTCACCCCCC GGAAGCCAGC AGTCAAAAAA GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA 
    GGAAAGGAGG GAGCTGCTGA GGGACCCCTG GATCCATCTG GTTACAATCC TGCCAAGAAC 
    AACTATCATC CCGTGGAAGA TGCCTGCTGG AAACCGGGCC AGAAGGTTCC TTACCTGGCT 
    GTGGCCCGGA CGTTTGAGAA GATCGAGGAG GTGTCTGCTC GGCTCCGGAT GGTGGAGACG 
    CTGAGCAACT TGCTGCGCTC CGTGGTGGCC CTGTCGCCTC CAGACCTCCT CCCTGTCCTC 
    TACCTCAGCC TCAACCACCT TGGGCCACCC CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT 
    GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT 
    GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG GTGGCCGAGA ACAGCCGCAG CACCCAGAGG 
    CTCATGCTGC CACCACCTCC GCTCACTGCC TCCGGGGTCT TCAGCAAGTT CCGCGACATC 
    GCCAGGCTCA CTGGCAGTGC TTCCACAGCC AAGAAGATAG ACATCATCAA AGGCCTCTTT 
    GTGGCCTGCC GCCACTCAGA AGCCCGGTTC ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC 
    CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT GCCCTCTCCC AGGCAGTGAG CCTCACGCCC 
    CCGGGCCAAG AATTCCCACC AGCCATGGTG GATGCTGGGA AGGGCAAGAC AGCAGAGGCC 
    AGAAAGACGT GGCTGGAGGA GCAAGGCATG ATCCTGAAGC AGACGTTCTG CGAGGTTCCC 
    GACCTGGACC GAATTATCCC CGTGCTGCTG GAGCACGGCC TGGAACGTCT CCCGGAGCAC 
    TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA CCAATGTTGG CCCATCCCAC CCGGGGCATC 
    AGCGAGGTCC TGAAACGCTT TGAGGAGGCA GCTTTCACCT GCGAATACAA ATATGACGGG 
    CAGAGGGCAC AGATCCACGC CCTGGAAGGC GGGGAGGTGA AGATCTTCAG CAGGAATCAG 
    GAAGACAACA CTGGGAAGTA CCCGGACATC ATCAGCCGCA TCCCCAAGAT TAAACTCCCA 
    TCGGTCACAT CCTTCATCCT GGACACCGAA GCCGTGGCTT GGGACCGGGA AAAGAAGCAG 
    ATCCAGCCAT TCCAAGTGCT CACCACCCGC AAACGCAAGG AGGTGGATGC GTCTGAGATC 
    CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC CTCATCTACC TCAATGGAGA GTCCCTGGTA 
    CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG CTCCGGGAGA ACTTTGTGGA GACAGAGGGC 
    GAGTTTGTCT TCGCCACCTC CCTGGACACC AAGGACATCG AGCAGATCGC CGAGTTCCTG 
    GAGCAGTCAG TGAAAGACTC CTGCGAGGGG CTGATGGTGA AGACCCTGGA TGTTGATGCC 
    ACCTACGAGA TCGCCAAGAG ATCGCACAAC TGGCTCAAGC TGAAGAAGGA CTACCTTGAT 
    GGCGTGGGTG ACACCCTGGA CCTGGTGGTG ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG 
    GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC TCCTACGACG AGGACAGTGA GGAGCTGCAG 
    GCCATATGCA AGCTTGGAAC TGGCTTCAGT GATGAGGAGC TGGAGGAGCA TCACCAGAGC 
    CTCAAGGCGC TGGTGCTGCC CAGCCCACGC CCTTACGTGC GGATAGATGG CGCTGTGATT 
    CCCGACCACT GGCTGGACCC CAGCGCTGTG TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC 
    TCTCCCATCT ACCCTGCTGC GCGGGGCCTG GTGGATAGTG ACAAGGGCAT CTCCCTTCGC 
    TTCCCTCGGT TTATTCGAGT CCGTGAAGAC AAGCAGCCGG AGCAGGCCAC CACCAGTGCT 
    CAGGTGGCCT GTTTGTACCG GAAGCAAAGT CAGATTCAGA ACCAACAAGG CGAGGACTCA 
    GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275992.1
    CDS421..3087
    Translation

    Target ORF information:

    RefSeq Version NM_001289063.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001289063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAGGA GACCCCGAAA GAAAGCCTCA CAGAGGCTGA AGTGGCCACA 
    GAGAAGGAAG GAGAAGACGG GGACCAGCCC ACCACGCCTC CCAAGCCCCT AAAGACCTCC 
    AAAGCAGAGA CCCCGACGGA AAGCGTTTCA GAGCCTGAGG TGGCCACGAA GCAGGAACTG 
    CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC CGCAGAGCTC CCAAGACGCT CAGCAGCTTC 
    TTCACCCCCC GGAAGCCAGC AGTCAAAAAA GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA 
    GGAAAGGAGG GAGCTGCTGA GGGACCCCTG GATCCATCTG GTTACAATCC TGCCAAGAAC 
    AACTATCATC CCGTGGAAGA TGCCTGCTGG AAACCGGGCC AGAAGGTTCC TTACCTGGCT 
    GTGGCCCGGA CGTTTGAGAA GATCGAGGAG GTGTCTGCTC GGCTCCGGAT GGTGGAGACG 
    CTGAGCAACT TGCTGCGCTC CGTGGTGGCC CTGTCGCCTC CAGACCTCCT CCCTGTCCTC 
    TACCTCAGCC TCAACCACCT TGGGCCACCC CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT 
    GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT 
    GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG GTGGCCGAGA ACAGCCGCAG CACCCAGAGG 
    CTCATGCTGC CACCACCTCC GCTCACTGCC TCCGGGGTCT TCAGCAAGTT CCGCGACATC 
    GCCAGGCTCA CTGGCAGTGC TTCCACAGCC AAGAAGATAG ACATCATCAA AGGCCTCTTT 
    GTGGCCTGCC GCCACTCAGA AGCCCGGTTC ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC 
    CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT GCCCTCTCCC AGGCAGTGAG CCTCACGCCC 
    CCGGGCCAAG AATTCCCACC AGCCATGGTG GATGCTGGGA AGGGCAAGAC AGCAGAGGCC 
    AGAAAGACGT GGCTGGAGGA GCAAGGCATG ATCCTGAAGC AGACGTTCTG CGAGGTTCCC 
    GACCTGGACC GAATTATCCC CGTGCTGCTG GAGCACGGCC TGGAACGTCT CCCGGAGCAC 
    TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA CCAATGTTGG CCCATCCCAC CCGGGGCATC 
    AGCGAGGTCC TGAAACGCTT TGAGGAGGCA GCTTTCACCT GCGAATACAA ATATGACGGG 
    CAGAGGGCAC AGATCCACGC CCTGGAAGGC GGGGAGGTGA AGATCTTCAG CAGGAATCAG 
    GAAGACAACA CTGGGAAGTA CCCGGACATC ATCAGCCGCA TCCCCAAGAT TAAACTCCCA 
    TCGGTCACAT CCTTCATCCT GGACACCGAA GCCGTGGCTT GGGACCGGGA AAAGAAGCAG 
    ATCCAGCCAT TCCAAGTGCT CACCACCCGC AAACGCAAGG AGGTGGATGC GTCTGAGATC 
    CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC CTCATCTACC TCAATGGAGA GTCCCTGGTA 
    CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG CTCCGGGAGA ACTTTGTGGA GACAGAGGGC 
    GAGTTTGTCT TCGCCACCTC CCTGGACACC AAGGACATCG AGCAGATCGC CGAGTTCCTG 
    GAGCAGTCAG TGAAAGACTC CTGCGAGGGG CTGATGGTGA AGACCCTGGA TGTTGATGCC 
    ACCTACGAGA TCGCCAAGAG ATCGCACAAC TGGCTCAAGC TGAAGAAGGA CTACCTTGAT 
    GGCGTGGGTG ACACCCTGGA CCTGGTGGTG ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG 
    GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC TCCTACGACG AGGACAGTGA GGAGCTGCAG 
    GCCATATGCA AGCTTGGAAC TGGCTTCAGT GATGAGGAGC TGGAGGAGCA TCACCAGAGC 
    CTCAAGGCGC TGGTGCTGCC CAGCCCACGC CCTTACGTGC GGATAGATGG CGCTGTGATT 
    CCCGACCACT GGCTGGACCC CAGCGCTGTG TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC 
    TCTCCCATCT ACCCTGCTGC GCGGGGCCTG GTGGATAGTG ACAAGGGCAT CTCCCTTCGC 
    TTCCCTCGGT TTATTCGAGT CCGTGAAGAC AAGCAGCCGG AGCAGGCCAC CACCAGTGCT 
    CAGGTGGCCT GTTTGTACCG GAAGCAAAGT CAGATTCAGA ACCAACAAGG CGAGGACTCA 
    GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001289063.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275992.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu14238
    Clone ID Related Accession (Same CDS sequence) NM_001289063.1 , NM_001289063.2
    Accession Version NM_001289063.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108318.1 and AK300370.1. On May 31, 2019 this sequence version replaced NM_001289063.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300370.1, SRR1803612.252686.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAGGA GACCCCGAAA GAAAGCCTCA CAGAGGCTGA AGTGGCCACA 
    GAGAAGGAAG GAGAAGACGG GGACCAGCCC ACCACGCCTC CCAAGCCCCT AAAGACCTCC 
    AAAGCAGAGA CCCCGACGGA AAGCGTTTCA GAGCCTGAGG TGGCCACGAA GCAGGAACTG 
    CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC CGCAGAGCTC CCAAGACGCT CAGCAGCTTC 
    TTCACCCCCC GGAAGCCAGC AGTCAAAAAA GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA 
    GGAAAGGAGG GAGCTGCTGA GGGACCCCTG GATCCATCTG GTTACAATCC TGCCAAGAAC 
    AACTATCATC CCGTGGAAGA TGCCTGCTGG AAACCGGGCC AGAAGGTTCC TTACCTGGCT 
    GTGGCCCGGA CGTTTGAGAA GATCGAGGAG GTGTCTGCTC GGCTCCGGAT GGTGGAGACG 
    CTGAGCAACT TGCTGCGCTC CGTGGTGGCC CTGTCGCCTC CAGACCTCCT CCCTGTCCTC 
    TACCTCAGCC TCAACCACCT TGGGCCACCC CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT 
    GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT 
    GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG GTGGCCGAGA ACAGCCGCAG CACCCAGAGG 
    CTCATGCTGC CACCACCTCC GCTCACTGCC TCCGGGGTCT TCAGCAAGTT CCGCGACATC 
    GCCAGGCTCA CTGGCAGTGC TTCCACAGCC AAGAAGATAG ACATCATCAA AGGCCTCTTT 
    GTGGCCTGCC GCCACTCAGA AGCCCGGTTC ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC 
    CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT GCCCTCTCCC AGGCAGTGAG CCTCACGCCC 
    CCGGGCCAAG AATTCCCACC AGCCATGGTG GATGCTGGGA AGGGCAAGAC AGCAGAGGCC 
    AGAAAGACGT GGCTGGAGGA GCAAGGCATG ATCCTGAAGC AGACGTTCTG CGAGGTTCCC 
    GACCTGGACC GAATTATCCC CGTGCTGCTG GAGCACGGCC TGGAACGTCT CCCGGAGCAC 
    TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA CCAATGTTGG CCCATCCCAC CCGGGGCATC 
    AGCGAGGTCC TGAAACGCTT TGAGGAGGCA GCTTTCACCT GCGAATACAA ATATGACGGG 
    CAGAGGGCAC AGATCCACGC CCTGGAAGGC GGGGAGGTGA AGATCTTCAG CAGGAATCAG 
    GAAGACAACA CTGGGAAGTA CCCGGACATC ATCAGCCGCA TCCCCAAGAT TAAACTCCCA 
    TCGGTCACAT CCTTCATCCT GGACACCGAA GCCGTGGCTT GGGACCGGGA AAAGAAGCAG 
    ATCCAGCCAT TCCAAGTGCT CACCACCCGC AAACGCAAGG AGGTGGATGC GTCTGAGATC 
    CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC CTCATCTACC TCAATGGAGA GTCCCTGGTA 
    CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG CTCCGGGAGA ACTTTGTGGA GACAGAGGGC 
    GAGTTTGTCT TCGCCACCTC CCTGGACACC AAGGACATCG AGCAGATCGC CGAGTTCCTG 
    GAGCAGTCAG TGAAAGACTC CTGCGAGGGG CTGATGGTGA AGACCCTGGA TGTTGATGCC 
    ACCTACGAGA TCGCCAAGAG ATCGCACAAC TGGCTCAAGC TGAAGAAGGA CTACCTTGAT 
    GGCGTGGGTG ACACCCTGGA CCTGGTGGTG ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG 
    GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC TCCTACGACG AGGACAGTGA GGAGCTGCAG 
    GCCATATGCA AGCTTGGAAC TGGCTTCAGT GATGAGGAGC TGGAGGAGCA TCACCAGAGC 
    CTCAAGGCGC TGGTGCTGCC CAGCCCACGC CCTTACGTGC GGATAGATGG CGCTGTGATT 
    CCCGACCACT GGCTGGACCC CAGCGCTGTG TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC 
    TCTCCCATCT ACCCTGCTGC GCGGGGCCTG GTGGATAGTG ACAAGGGCAT CTCCCTTCGC 
    TTCCCTCGGT TTATTCGAGT CCGTGAAGAC AAGCAGCCGG AGCAGGCCAC CACCAGTGCT 
    CAGGTGGCCT GTTTGTACCG GAAGCAAAGT CAGATTCAGA ACCAACAAGG CGAGGACTCA 
    GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275992.1
    CDS162..2828
    Translation

    Target ORF information:

    RefSeq Version NM_001289063.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001289063.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAGGA GACCCCGAAA GAAAGCCTCA CAGAGGCTGA AGTGGCCACA 
    GAGAAGGAAG GAGAAGACGG GGACCAGCCC ACCACGCCTC CCAAGCCCCT AAAGACCTCC 
    AAAGCAGAGA CCCCGACGGA AAGCGTTTCA GAGCCTGAGG TGGCCACGAA GCAGGAACTG 
    CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC CGCAGAGCTC CCAAGACGCT CAGCAGCTTC 
    TTCACCCCCC GGAAGCCAGC AGTCAAAAAA GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA 
    GGAAAGGAGG GAGCTGCTGA GGGACCCCTG GATCCATCTG GTTACAATCC TGCCAAGAAC 
    AACTATCATC CCGTGGAAGA TGCCTGCTGG AAACCGGGCC AGAAGGTTCC TTACCTGGCT 
    GTGGCCCGGA CGTTTGAGAA GATCGAGGAG GTGTCTGCTC GGCTCCGGAT GGTGGAGACG 
    CTGAGCAACT TGCTGCGCTC CGTGGTGGCC CTGTCGCCTC CAGACCTCCT CCCTGTCCTC 
    TACCTCAGCC TCAACCACCT TGGGCCACCC CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT 
    GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT 
    GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG GTGGCCGAGA ACAGCCGCAG CACCCAGAGG 
    CTCATGCTGC CACCACCTCC GCTCACTGCC TCCGGGGTCT TCAGCAAGTT CCGCGACATC 
    GCCAGGCTCA CTGGCAGTGC TTCCACAGCC AAGAAGATAG ACATCATCAA AGGCCTCTTT 
    GTGGCCTGCC GCCACTCAGA AGCCCGGTTC ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC 
    CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT GCCCTCTCCC AGGCAGTGAG CCTCACGCCC 
    CCGGGCCAAG AATTCCCACC AGCCATGGTG GATGCTGGGA AGGGCAAGAC AGCAGAGGCC 
    AGAAAGACGT GGCTGGAGGA GCAAGGCATG ATCCTGAAGC AGACGTTCTG CGAGGTTCCC 
    GACCTGGACC GAATTATCCC CGTGCTGCTG GAGCACGGCC TGGAACGTCT CCCGGAGCAC 
    TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA CCAATGTTGG CCCATCCCAC CCGGGGCATC 
    AGCGAGGTCC TGAAACGCTT TGAGGAGGCA GCTTTCACCT GCGAATACAA ATATGACGGG 
    CAGAGGGCAC AGATCCACGC CCTGGAAGGC GGGGAGGTGA AGATCTTCAG CAGGAATCAG 
    GAAGACAACA CTGGGAAGTA CCCGGACATC ATCAGCCGCA TCCCCAAGAT TAAACTCCCA 
    TCGGTCACAT CCTTCATCCT GGACACCGAA GCCGTGGCTT GGGACCGGGA AAAGAAGCAG 
    ATCCAGCCAT TCCAAGTGCT CACCACCCGC AAACGCAAGG AGGTGGATGC GTCTGAGATC 
    CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC CTCATCTACC TCAATGGAGA GTCCCTGGTA 
    CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG CTCCGGGAGA ACTTTGTGGA GACAGAGGGC 
    GAGTTTGTCT TCGCCACCTC CCTGGACACC AAGGACATCG AGCAGATCGC CGAGTTCCTG 
    GAGCAGTCAG TGAAAGACTC CTGCGAGGGG CTGATGGTGA AGACCCTGGA TGTTGATGCC 
    ACCTACGAGA TCGCCAAGAG ATCGCACAAC TGGCTCAAGC TGAAGAAGGA CTACCTTGAT 
    GGCGTGGGTG ACACCCTGGA CCTGGTGGTG ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG 
    GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC TCCTACGACG AGGACAGTGA GGAGCTGCAG 
    GCCATATGCA AGCTTGGAAC TGGCTTCAGT GATGAGGAGC TGGAGGAGCA TCACCAGAGC 
    CTCAAGGCGC TGGTGCTGCC CAGCCCACGC CCTTACGTGC GGATAGATGG CGCTGTGATT 
    CCCGACCACT GGCTGGACCC CAGCGCTGTG TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC 
    TCTCCCATCT ACCCTGCTGC GCGGGGCCTG GTGGATAGTG ACAAGGGCAT CTCCCTTCGC 
    TTCCCTCGGT TTATTCGAGT CCGTGAAGAC AAGCAGCCGG AGCAGGCCAC CACCAGTGCT 
    CAGGTGGCCT GTTTGTACCG GAAGCAAAGT CAGATTCAGA ACCAACAAGG CGAGGACTCA 
    GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001289064.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275993.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu14327
    Clone ID Related Accession (Same CDS sequence) NM_001289064.1
    Accession Version NM_001289064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2556bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC108318.1 and AK297296.1. On Jan 15, 2014 this sequence version replaced XM_005258936.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297296.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG AAGCAGAGAC CCCGACGGAA AGCGTTTCAG AGCCTGAGGT GGCCACGAAG 
    CAGGAACTGC AGGAGGAGGA AGAGCAGACC AAGCCTCCCC GCAGAGCTCC CAAGACGCTC 
    AGCAGCTTCT TCACCCCCCG GAAGCCAGCA GTCAAAAAAG AAGTGAAGGA AGAGGAGCCA 
    GGGGCTCCAG GAAAGGAGGG AGCTGCTGAG GGACCCCTGG ATCCATCTGG TTACAATCCT 
    GCCAAGAACA ACTATCATCC CGTGGAAGAT GCCTGCTGGA AACCGGGCCA GAAGGTTCCT 
    TACCTGGCTG TGGCCCGGAC GTTTGAGAAG ATCGAGGAGG TGTCTGCTCG GCTCCGGATG 
    GTGGAGACGC TGAGCAACTT GCTGCGCTCC GTGGTGGCCC TGTCGCCTCC AGACCTCCTC 
    CCTGTCCTCT ACCTCAGCCT CAACCACCTT GGGCCACCCC AGCAGGGCCT GGAGCTTGGC 
    GTGGGTGATG GTGTCCTTCT CAAGGCAGTG GCCCAGGCCA CAGGTCGGCA GCTGGAGTCC 
    GTCCGGGCTG AGGCAGCCGA GAAAGGCGAC GTGGGGCTGG TGGCCGAGAA CAGCCGCAGC 
    ACCCAGAGGC TCATGCTGCC ACCACCTCCG CTCACTGCCT CCGGGGTCTT CAGCAAGTTC 
    CGCGACATCG CCAGGCTCAC TGGCAGTGCT TCCACAGCCA AGAAGATAGA CATCATCAAA 
    GGCCTCTTTG TGGCCTGCCG CCACTCAGAA GCCCGGTTCA TCGCTAGGTC CCTGAGCGGA 
    CGGCTGCGCC TTGGGCTGGC AGAGCAGTCG GTGCTGGCTG CCCTCTCCCA GGCAGTGAGC 
    CTCACGCCCC CGGGCCAAGA ATTCCCACCA GCCATGGTGG ATGCTGGGAA GGGCAAGACA 
    GCAGAGGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACGTTCTGC 
    GAGGTTCCCG ACCTGGACCG AATTATCCCC GTGCTGCTGG AGCACGGCCT GGAACGTCTC 
    CCGGAGCACT GCAAGCTGAG CCCAGGGATT CCCCTGAAAC CAATGTTGGC CCATCCCACC 
    CGGGGCATCA GCGAGGTCCT GAAACGCTTT GAGGAGGCAG CTTTCACCTG CGAATACAAA 
    TATGACGGGC AGAGGGCACA GATCCACGCC CTGGAAGGCG GGGAGGTGAA GATCTTCAGC 
    AGGAATCAGG AAGACAACAC TGGGAAGTAC CCGGACATCA TCAGCCGCAT CCCCAAGATT 
    AAACTCCCAT CGGTCACATC CTTCATCCTG GACACCGAAG CCGTGGCTTG GGACCGGGAA 
    AAGAAGCAGA TCCAGCCATT CCAAGTGCTC ACCACCCGCA AACGCAAGGA GGTGGATGCG 
    TCTGAGATCC AGGTGCAGGT GTGTTTGTAC GCCTTCGACC TCATCTACCT CAATGGAGAG 
    TCCCTGGTAC GTGAGCCCCT TTCCCGGCGC CGGCAGCTGC TCCGGGAGAA CTTTGTGGAG 
    ACAGAGGGCG AGTTTGTCTT CGCCACCTCC CTGGACACCA AGGACATCGA GCAGATCGCC 
    GAGTTCCTGG AGCAGTCAGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT 
    GTTGATGCCA CCTACGAGAT CGCCAAGAGA TCGCACAACT GGCTCAAGCT GAAGAAGGAC 
    TACCTTGATG GCGTGGGTGA CACCCTGGAC CTGGTGGTGA TCGGCGCCTA CCTGGGCCGG 
    GGGAAGCGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTACGACGA GGACAGTGAG 
    GAGCTGCAGG CCATATGCAA GCTTGGAACT GGCTTCAGTG ATGAGGAGCT GGAGGAGCAT 
    CACCAGAGCC TCAAGGCGCT GGTGCTGCCC AGCCCACGCC CTTACGTGCG GATAGATGGC 
    GCTGTGATTC CCGACCACTG GCTGGACCCC AGCGCTGTGT GGGAGGTGAA GTGCGCTGAC 
    CTCTCCCTCT CTCCCATCTA CCCTGCTGCG CGGGGCCTGG TGGATAGTGA CAAGGGCATC 
    TCCCTTCGCT TCCCTCGGTT TATTCGAGTC CGTGAAGACA AGCAGCCGGA GCAGGCCACC 
    ACCAGTGCTC AGGTGGCCTG TTTGTACCGG AAGCAAAGTC AGATTCAGAA CCAACAAGGC 
    GAGGACTCAG GCTCTGACCC TGAAGATACC TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001275993.1
    CDS421..2976
    Translation

    Target ORF information:

    RefSeq Version NM_001289064.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001289064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG AAGCAGAGAC CCCGACGGAA AGCGTTTCAG AGCCTGAGGT GGCCACGAAG 
    CAGGAACTGC AGGAGGAGGA AGAGCAGACC AAGCCTCCCC GCAGAGCTCC CAAGACGCTC 
    AGCAGCTTCT TCACCCCCCG GAAGCCAGCA GTCAAAAAAG AAGTGAAGGA AGAGGAGCCA 
    GGGGCTCCAG GAAAGGAGGG AGCTGCTGAG GGACCCCTGG ATCCATCTGG TTACAATCCT 
    GCCAAGAACA ACTATCATCC CGTGGAAGAT GCCTGCTGGA AACCGGGCCA GAAGGTTCCT 
    TACCTGGCTG TGGCCCGGAC GTTTGAGAAG ATCGAGGAGG TGTCTGCTCG GCTCCGGATG 
    GTGGAGACGC TGAGCAACTT GCTGCGCTCC GTGGTGGCCC TGTCGCCTCC AGACCTCCTC 
    CCTGTCCTCT ACCTCAGCCT CAACCACCTT GGGCCACCCC AGCAGGGCCT GGAGCTTGGC 
    GTGGGTGATG GTGTCCTTCT CAAGGCAGTG GCCCAGGCCA CAGGTCGGCA GCTGGAGTCC 
    GTCCGGGCTG AGGCAGCCGA GAAAGGCGAC GTGGGGCTGG TGGCCGAGAA CAGCCGCAGC 
    ACCCAGAGGC TCATGCTGCC ACCACCTCCG CTCACTGCCT CCGGGGTCTT CAGCAAGTTC 
    CGCGACATCG CCAGGCTCAC TGGCAGTGCT TCCACAGCCA AGAAGATAGA CATCATCAAA 
    GGCCTCTTTG TGGCCTGCCG CCACTCAGAA GCCCGGTTCA TCGCTAGGTC CCTGAGCGGA 
    CGGCTGCGCC TTGGGCTGGC AGAGCAGTCG GTGCTGGCTG CCCTCTCCCA GGCAGTGAGC 
    CTCACGCCCC CGGGCCAAGA ATTCCCACCA GCCATGGTGG ATGCTGGGAA GGGCAAGACA 
    GCAGAGGCCA GAAAGACGTG GCTGGAGGAG CAAGGCATGA TCCTGAAGCA GACGTTCTGC 
    GAGGTTCCCG ACCTGGACCG AATTATCCCC GTGCTGCTGG AGCACGGCCT GGAACGTCTC 
    CCGGAGCACT GCAAGCTGAG CCCAGGGATT CCCCTGAAAC CAATGTTGGC CCATCCCACC 
    CGGGGCATCA GCGAGGTCCT GAAACGCTTT GAGGAGGCAG CTTTCACCTG CGAATACAAA 
    TATGACGGGC AGAGGGCACA GATCCACGCC CTGGAAGGCG GGGAGGTGAA GATCTTCAGC 
    AGGAATCAGG AAGACAACAC TGGGAAGTAC CCGGACATCA TCAGCCGCAT CCCCAAGATT 
    AAACTCCCAT CGGTCACATC CTTCATCCTG GACACCGAAG CCGTGGCTTG GGACCGGGAA 
    AAGAAGCAGA TCCAGCCATT CCAAGTGCTC ACCACCCGCA AACGCAAGGA GGTGGATGCG 
    TCTGAGATCC AGGTGCAGGT GTGTTTGTAC GCCTTCGACC TCATCTACCT CAATGGAGAG 
    TCCCTGGTAC GTGAGCCCCT TTCCCGGCGC CGGCAGCTGC TCCGGGAGAA CTTTGTGGAG 
    ACAGAGGGCG AGTTTGTCTT CGCCACCTCC CTGGACACCA AGGACATCGA GCAGATCGCC 
    GAGTTCCTGG AGCAGTCAGT GAAAGACTCC TGCGAGGGGC TGATGGTGAA GACCCTGGAT 
    GTTGATGCCA CCTACGAGAT CGCCAAGAGA TCGCACAACT GGCTCAAGCT GAAGAAGGAC 
    TACCTTGATG GCGTGGGTGA CACCCTGGAC CTGGTGGTGA TCGGCGCCTA CCTGGGCCGG 
    GGGAAGCGGG CCGGCCGGTA CGGGGGCTTC CTGCTGGCCT CCTACGACGA GGACAGTGAG 
    GAGCTGCAGG CCATATGCAA GCTTGGAACT GGCTTCAGTG ATGAGGAGCT GGAGGAGCAT 
    CACCAGAGCC TCAAGGCGCT GGTGCTGCCC AGCCCACGCC CTTACGTGCG GATAGATGGC 
    GCTGTGATTC CCGACCACTG GCTGGACCCC AGCGCTGTGT GGGAGGTGAA GTGCGCTGAC 
    CTCTCCCTCT CTCCCATCTA CCCTGCTGCG CGGGGCCTGG TGGATAGTGA CAAGGGCATC 
    TCCCTTCGCT TCCCTCGGTT TATTCGAGTC CGTGAAGACA AGCAGCCGGA GCAGGCCACC 
    ACCAGTGCTC AGGTGGCCTG TTTGTACCGG AAGCAAAGTC AGATTCAGAA CCAACAAGGC 
    GAGGACTCAG GCTCTGACCC TGAAGATACC TACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001320970.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307899.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA.
    Price & Availability↑
    CloneID OHu52262
    Clone ID Related Accession (Same CDS sequence) NM_001320970.2 , NM_001320970.1
    Accession Version NM_001320970.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011466.6, KC877741.1 and BC108318.1. On May 31, 2019 this sequence version replaced NM_001320970.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.27718.1, SRR1803615.179219.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAGGA GACCCCGAAA 
    GAAAGCCTCA CAGAGGCTGA AGTGGCCACA GAGAAGGAAG GAGAAGACGG GGACCAGCCC 
    ACCACGCCTC CCAAGCCCCT AAAGACCTCC AAAGCAGAGA CCCCGACGGA AAGCGTTTCA 
    GAGCCTGAGG TGGCCACGAA GCAGGAACTG CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC 
    CGCAGAGCTC CCAAGACGCT CAGCAGCTTC TTCACCCCCC GGAAGCCAGC AGTCAAAAAA 
    GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA GGAAAGGAGG GAGCTGCTGA GGGACCCCTG 
    GATCCATCTG GTTACAATCC TGCCAAGAAC AACTATCATC CCGTGGAAGA TGCCTGCTGG 
    AAACCGGGCC AGAAGGTTCC TTACCTGGCT GTGGCCCGGA CGTTTGAGAA GATCGAGGAG 
    GTGTCTGCTC GGCTCCGGAT GGTGGAGACG CTGAGCAACT TGCTGCGCTC CGTGGTGGCC 
    CTGTCGCCTC CAGACCTCCT CCCTGTCCTC TACCTCAGCC TCAACCACCT TGGGCCACCC 
    CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC 
    ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG 
    GTGGCCGAGA ACAGCCGCAG CACCCAGAGG CTCATGCTGC CACCACCTCC GCTCACTGCC 
    TCCGGGGTCT TCAGCAAGTT CCGCGACATC GCCAGGCTCA CTGGCAGTGC TTCCACAGCC 
    AAGAAGATAG ACATCATCAA AGGCCTCTTT GTGGCCTGCC GCCACTCAGA AGCCCGGTTC 
    ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT 
    GCCCTCTCCC AGGCAGTGAG CCTCACGCCC CCGGGCCAAG AATTCCCACC AGCCATGGTG 
    GATGCTGGGA AGGGCAAGAC AGCAGAGGCC AGAAAGACGT GGCTGGAGGA GCAAGGCATG 
    ATCCTGAAGC AGACGTTCTG CGAGGTTCCC GACCTGGACC GAATTATCCC CGTGCTGCTG 
    GAGCACGGCC TGGAACGTCT CCCGGAGCAC TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA 
    CCAATGTTGG CCCATCCCAC CCGGGGCATC AGCGAGGTCC TGAAACGCTT TGAGGAGGCA 
    GCTTTCACCT GCGAATACAA ATATGACGGG CAGAGGGCAC AGATCCACGC CCTGGAAGGC 
    GGGGAGGTGA AGATCTTCAG CAGGAATCAG GAAGACAACA CTGGGAAGTA CCCGGACATC 
    ATCAGCCGCA TCCCCAAGAT TAAACTCCCA TCGGTCACAT CCTTCATCCT GGACACCGAA 
    GCCGTGGCTT GGGACCGGGA AAAGAAGCAG ATCCAGCCAT TCCAAGTGCT CACCACCCGC 
    AAACGCAAGG AGGTGGATGC GTCTGAGATC CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC 
    CTCATCTACC TCAATGGAGA GTCCCTGGTA CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG 
    CTCCGGGAGA ACTTTGTGGA GACAGAGGGC GAGTTTGTCT TCGCCACCTC CCTGGACACC 
    AAGGACATCG AGCAGATCGC CGAGTTCCTG GAGCAGTCAG TGAAAGACTC CTGCGAGGGG 
    CTGATGGTGA AGACCCTGGA TGTTGATGCC ACCTACGAGA TCGCCAAGAG ATCGCACAAC 
    TGGCTCAAGC TGAAGAAGGA CTACCTTGAT GGCGTGGGTG ACACCCTGGA CCTGGTGGTG 
    ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC 
    TCCTACGACG AGGACAGTGA GGAGCTGCAG GCCATATGCA AGCTTGGAAC TGGCTTCAGT 
    GATGAGGAGC TGGAGGAGCA TCACCAGAGC CTCAAGGCGC TGGTGCTGCC CAGCCCACGC 
    CCTTACGTGC GGATAGATGG CGCTGTGATT CCCGACCACT GGCTGGACCC CAGCGCTGTG 
    TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC TCTCCCATCT ACCCTGCTGC GCGGGGCCTG 
    GTGGATAGTG ACAAGGGCAT CTCCCTTCGC TTCCCTCGGT TTATTCGAGT CCGTGAAGAC 
    AAGCAGCCGG AGCAGGCCAC CACCAGTGCT CAGGTGGCCT GTTTGTACCG GAAGCAAAGT 
    CAGATTCAGA ACCAACAAGG CGAGGACTCA GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307899.1
    CDS162..2918
    Translation

    Target ORF information:

    RefSeq Version NM_001320970.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001320970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAGGA GACCCCGAAA 
    GAAAGCCTCA CAGAGGCTGA AGTGGCCACA GAGAAGGAAG GAGAAGACGG GGACCAGCCC 
    ACCACGCCTC CCAAGCCCCT AAAGACCTCC AAAGCAGAGA CCCCGACGGA AAGCGTTTCA 
    GAGCCTGAGG TGGCCACGAA GCAGGAACTG CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC 
    CGCAGAGCTC CCAAGACGCT CAGCAGCTTC TTCACCCCCC GGAAGCCAGC AGTCAAAAAA 
    GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA GGAAAGGAGG GAGCTGCTGA GGGACCCCTG 
    GATCCATCTG GTTACAATCC TGCCAAGAAC AACTATCATC CCGTGGAAGA TGCCTGCTGG 
    AAACCGGGCC AGAAGGTTCC TTACCTGGCT GTGGCCCGGA CGTTTGAGAA GATCGAGGAG 
    GTGTCTGCTC GGCTCCGGAT GGTGGAGACG CTGAGCAACT TGCTGCGCTC CGTGGTGGCC 
    CTGTCGCCTC CAGACCTCCT CCCTGTCCTC TACCTCAGCC TCAACCACCT TGGGCCACCC 
    CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC 
    ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG 
    GTGGCCGAGA ACAGCCGCAG CACCCAGAGG CTCATGCTGC CACCACCTCC GCTCACTGCC 
    TCCGGGGTCT TCAGCAAGTT CCGCGACATC GCCAGGCTCA CTGGCAGTGC TTCCACAGCC 
    AAGAAGATAG ACATCATCAA AGGCCTCTTT GTGGCCTGCC GCCACTCAGA AGCCCGGTTC 
    ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT 
    GCCCTCTCCC AGGCAGTGAG CCTCACGCCC CCGGGCCAAG AATTCCCACC AGCCATGGTG 
    GATGCTGGGA AGGGCAAGAC AGCAGAGGCC AGAAAGACGT GGCTGGAGGA GCAAGGCATG 
    ATCCTGAAGC AGACGTTCTG CGAGGTTCCC GACCTGGACC GAATTATCCC CGTGCTGCTG 
    GAGCACGGCC TGGAACGTCT CCCGGAGCAC TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA 
    CCAATGTTGG CCCATCCCAC CCGGGGCATC AGCGAGGTCC TGAAACGCTT TGAGGAGGCA 
    GCTTTCACCT GCGAATACAA ATATGACGGG CAGAGGGCAC AGATCCACGC CCTGGAAGGC 
    GGGGAGGTGA AGATCTTCAG CAGGAATCAG GAAGACAACA CTGGGAAGTA CCCGGACATC 
    ATCAGCCGCA TCCCCAAGAT TAAACTCCCA TCGGTCACAT CCTTCATCCT GGACACCGAA 
    GCCGTGGCTT GGGACCGGGA AAAGAAGCAG ATCCAGCCAT TCCAAGTGCT CACCACCCGC 
    AAACGCAAGG AGGTGGATGC GTCTGAGATC CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC 
    CTCATCTACC TCAATGGAGA GTCCCTGGTA CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG 
    CTCCGGGAGA ACTTTGTGGA GACAGAGGGC GAGTTTGTCT TCGCCACCTC CCTGGACACC 
    AAGGACATCG AGCAGATCGC CGAGTTCCTG GAGCAGTCAG TGAAAGACTC CTGCGAGGGG 
    CTGATGGTGA AGACCCTGGA TGTTGATGCC ACCTACGAGA TCGCCAAGAG ATCGCACAAC 
    TGGCTCAAGC TGAAGAAGGA CTACCTTGAT GGCGTGGGTG ACACCCTGGA CCTGGTGGTG 
    ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC 
    TCCTACGACG AGGACAGTGA GGAGCTGCAG GCCATATGCA AGCTTGGAAC TGGCTTCAGT 
    GATGAGGAGC TGGAGGAGCA TCACCAGAGC CTCAAGGCGC TGGTGCTGCC CAGCCCACGC 
    CCTTACGTGC GGATAGATGG CGCTGTGATT CCCGACCACT GGCTGGACCC CAGCGCTGTG 
    TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC TCTCCCATCT ACCCTGCTGC GCGGGGCCTG 
    GTGGATAGTG ACAAGGGCAT CTCCCTTCGC TTCCCTCGGT TTATTCGAGT CCGTGAAGAC 
    AAGCAGCCGG AGCAGGCCAC CACCAGTGCT CAGGTGGCCT GTTTGTACCG GAAGCAAAGT 
    CAGATTCAGA ACCAACAAGG CGAGGACTCA GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001320970.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307899.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA.
    Price & Availability↑
    CloneID OHu52262
    Clone ID Related Accession (Same CDS sequence) NM_001320970.2 , NM_001320970.1
    Accession Version NM_001320970.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-09
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011466.6, KC877741.1 and BC108318.1. On Mar 12, 2016 this sequence version replaced XM_006723215.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.24761.1, SRR1803613.27718.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAGGA GACCCCGAAA 
    GAAAGCCTCA CAGAGGCTGA AGTGGCCACA GAGAAGGAAG GAGAAGACGG GGACCAGCCC 
    ACCACGCCTC CCAAGCCCCT AAAGACCTCC AAAGCAGAGA CCCCGACGGA AAGCGTTTCA 
    GAGCCTGAGG TGGCCACGAA GCAGGAACTG CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC 
    CGCAGAGCTC CCAAGACGCT CAGCAGCTTC TTCACCCCCC GGAAGCCAGC AGTCAAAAAA 
    GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA GGAAAGGAGG GAGCTGCTGA GGGACCCCTG 
    GATCCATCTG GTTACAATCC TGCCAAGAAC AACTATCATC CCGTGGAAGA TGCCTGCTGG 
    AAACCGGGCC AGAAGGTTCC TTACCTGGCT GTGGCCCGGA CGTTTGAGAA GATCGAGGAG 
    GTGTCTGCTC GGCTCCGGAT GGTGGAGACG CTGAGCAACT TGCTGCGCTC CGTGGTGGCC 
    CTGTCGCCTC CAGACCTCCT CCCTGTCCTC TACCTCAGCC TCAACCACCT TGGGCCACCC 
    CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC 
    ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG 
    GTGGCCGAGA ACAGCCGCAG CACCCAGAGG CTCATGCTGC CACCACCTCC GCTCACTGCC 
    TCCGGGGTCT TCAGCAAGTT CCGCGACATC GCCAGGCTCA CTGGCAGTGC TTCCACAGCC 
    AAGAAGATAG ACATCATCAA AGGCCTCTTT GTGGCCTGCC GCCACTCAGA AGCCCGGTTC 
    ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT 
    GCCCTCTCCC AGGCAGTGAG CCTCACGCCC CCGGGCCAAG AATTCCCACC AGCCATGGTG 
    GATGCTGGGA AGGGCAAGAC AGCAGAGGCC AGAAAGACGT GGCTGGAGGA GCAAGGCATG 
    ATCCTGAAGC AGACGTTCTG CGAGGTTCCC GACCTGGACC GAATTATCCC CGTGCTGCTG 
    GAGCACGGCC TGGAACGTCT CCCGGAGCAC TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA 
    CCAATGTTGG CCCATCCCAC CCGGGGCATC AGCGAGGTCC TGAAACGCTT TGAGGAGGCA 
    GCTTTCACCT GCGAATACAA ATATGACGGG CAGAGGGCAC AGATCCACGC CCTGGAAGGC 
    GGGGAGGTGA AGATCTTCAG CAGGAATCAG GAAGACAACA CTGGGAAGTA CCCGGACATC 
    ATCAGCCGCA TCCCCAAGAT TAAACTCCCA TCGGTCACAT CCTTCATCCT GGACACCGAA 
    GCCGTGGCTT GGGACCGGGA AAAGAAGCAG ATCCAGCCAT TCCAAGTGCT CACCACCCGC 
    AAACGCAAGG AGGTGGATGC GTCTGAGATC CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC 
    CTCATCTACC TCAATGGAGA GTCCCTGGTA CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG 
    CTCCGGGAGA ACTTTGTGGA GACAGAGGGC GAGTTTGTCT TCGCCACCTC CCTGGACACC 
    AAGGACATCG AGCAGATCGC CGAGTTCCTG GAGCAGTCAG TGAAAGACTC CTGCGAGGGG 
    CTGATGGTGA AGACCCTGGA TGTTGATGCC ACCTACGAGA TCGCCAAGAG ATCGCACAAC 
    TGGCTCAAGC TGAAGAAGGA CTACCTTGAT GGCGTGGGTG ACACCCTGGA CCTGGTGGTG 
    ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC 
    TCCTACGACG AGGACAGTGA GGAGCTGCAG GCCATATGCA AGCTTGGAAC TGGCTTCAGT 
    GATGAGGAGC TGGAGGAGCA TCACCAGAGC CTCAAGGCGC TGGTGCTGCC CAGCCCACGC 
    CCTTACGTGC GGATAGATGG CGCTGTGATT CCCGACCACT GGCTGGACCC CAGCGCTGTG 
    TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC TCTCCCATCT ACCCTGCTGC GCGGGGCCTG 
    GTGGATAGTG ACAAGGGCAT CTCCCTTCGC TTCCCTCGGT TTATTCGAGT CCGTGAAGAC 
    AAGCAGCCGG AGCAGGCCAC CACCAGTGCT CAGGTGGCCT GTTTGTACCG GAAGCAAAGT 
    CAGATTCAGA ACCAACAAGG CGAGGACTCA GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307899.1
    CDS421..3177
    Translation

    Target ORF information:

    RefSeq Version NM_001320970.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001320970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAGGA GACCCCGAAA 
    GAAAGCCTCA CAGAGGCTGA AGTGGCCACA GAGAAGGAAG GAGAAGACGG GGACCAGCCC 
    ACCACGCCTC CCAAGCCCCT AAAGACCTCC AAAGCAGAGA CCCCGACGGA AAGCGTTTCA 
    GAGCCTGAGG TGGCCACGAA GCAGGAACTG CAGGAGGAGG AAGAGCAGAC CAAGCCTCCC 
    CGCAGAGCTC CCAAGACGCT CAGCAGCTTC TTCACCCCCC GGAAGCCAGC AGTCAAAAAA 
    GAAGTGAAGG AAGAGGAGCC AGGGGCTCCA GGAAAGGAGG GAGCTGCTGA GGGACCCCTG 
    GATCCATCTG GTTACAATCC TGCCAAGAAC AACTATCATC CCGTGGAAGA TGCCTGCTGG 
    AAACCGGGCC AGAAGGTTCC TTACCTGGCT GTGGCCCGGA CGTTTGAGAA GATCGAGGAG 
    GTGTCTGCTC GGCTCCGGAT GGTGGAGACG CTGAGCAACT TGCTGCGCTC CGTGGTGGCC 
    CTGTCGCCTC CAGACCTCCT CCCTGTCCTC TACCTCAGCC TCAACCACCT TGGGCCACCC 
    CAGCAGGGCC TGGAGCTTGG CGTGGGTGAT GGTGTCCTTC TCAAGGCAGT GGCCCAGGCC 
    ACAGGTCGGC AGCTGGAGTC CGTCCGGGCT GAGGCAGCCG AGAAAGGCGA CGTGGGGCTG 
    GTGGCCGAGA ACAGCCGCAG CACCCAGAGG CTCATGCTGC CACCACCTCC GCTCACTGCC 
    TCCGGGGTCT TCAGCAAGTT CCGCGACATC GCCAGGCTCA CTGGCAGTGC TTCCACAGCC 
    AAGAAGATAG ACATCATCAA AGGCCTCTTT GTGGCCTGCC GCCACTCAGA AGCCCGGTTC 
    ATCGCTAGGT CCCTGAGCGG ACGGCTGCGC CTTGGGCTGG CAGAGCAGTC GGTGCTGGCT 
    GCCCTCTCCC AGGCAGTGAG CCTCACGCCC CCGGGCCAAG AATTCCCACC AGCCATGGTG 
    GATGCTGGGA AGGGCAAGAC AGCAGAGGCC AGAAAGACGT GGCTGGAGGA GCAAGGCATG 
    ATCCTGAAGC AGACGTTCTG CGAGGTTCCC GACCTGGACC GAATTATCCC CGTGCTGCTG 
    GAGCACGGCC TGGAACGTCT CCCGGAGCAC TGCAAGCTGA GCCCAGGGAT TCCCCTGAAA 
    CCAATGTTGG CCCATCCCAC CCGGGGCATC AGCGAGGTCC TGAAACGCTT TGAGGAGGCA 
    GCTTTCACCT GCGAATACAA ATATGACGGG CAGAGGGCAC AGATCCACGC CCTGGAAGGC 
    GGGGAGGTGA AGATCTTCAG CAGGAATCAG GAAGACAACA CTGGGAAGTA CCCGGACATC 
    ATCAGCCGCA TCCCCAAGAT TAAACTCCCA TCGGTCACAT CCTTCATCCT GGACACCGAA 
    GCCGTGGCTT GGGACCGGGA AAAGAAGCAG ATCCAGCCAT TCCAAGTGCT CACCACCCGC 
    AAACGCAAGG AGGTGGATGC GTCTGAGATC CAGGTGCAGG TGTGTTTGTA CGCCTTCGAC 
    CTCATCTACC TCAATGGAGA GTCCCTGGTA CGTGAGCCCC TTTCCCGGCG CCGGCAGCTG 
    CTCCGGGAGA ACTTTGTGGA GACAGAGGGC GAGTTTGTCT TCGCCACCTC CCTGGACACC 
    AAGGACATCG AGCAGATCGC CGAGTTCCTG GAGCAGTCAG TGAAAGACTC CTGCGAGGGG 
    CTGATGGTGA AGACCCTGGA TGTTGATGCC ACCTACGAGA TCGCCAAGAG ATCGCACAAC 
    TGGCTCAAGC TGAAGAAGGA CTACCTTGAT GGCGTGGGTG ACACCCTGGA CCTGGTGGTG 
    ATCGGCGCCT ACCTGGGCCG GGGGAAGCGG GCCGGCCGGT ACGGGGGCTT CCTGCTGGCC 
    TCCTACGACG AGGACAGTGA GGAGCTGCAG GCCATATGCA AGCTTGGAAC TGGCTTCAGT 
    GATGAGGAGC TGGAGGAGCA TCACCAGAGC CTCAAGGCGC TGGTGCTGCC CAGCCCACGC 
    CCTTACGTGC GGATAGATGG CGCTGTGATT CCCGACCACT GGCTGGACCC CAGCGCTGTG 
    TGGGAGGTGA AGTGCGCTGA CCTCTCCCTC TCTCCCATCT ACCCTGCTGC GCGGGGCCTG 
    GTGGATAGTG ACAAGGGCAT CTCCCTTCGC TTCCCTCGGT TTATTCGAGT CCGTGAAGAC 
    AAGCAGCCGG AGCAGGCCAC CACCAGTGCT CAGGTGGCCT GTTTGTACCG GAAGCAAAGT 
    CAGATTCAGA ACCAACAAGG CGAGGACTCA GGCTCTGACC CTGAAGATAC CTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001320971.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307900.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA.
    Price & Availability↑
    CloneID OHu52263
    Clone ID Related Accession (Same CDS sequence) NM_001320971.1 , NM_001320971.2
    Accession Version NM_001320971.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-04
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011466.6, KC877741.1 and BC108318.1. On Mar 12, 2016 this sequence version replaced XM_005258934.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.8621.1, SRR1660805.135014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAAGA GGAGACCCCG AAAGAAAGCC TCACAGAGGC TGAAGTGGCC 
    ACAGAGAAGG AAGGAGAAGA CGGGGACCAG CCCACCACGC CTCCCAAGCC CCTAAAGACC 
    TCCAAAGCAG AGACCCCGAC GGAAAGCGTT TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA 
    CTGCAGGAGG AGGAAGAGCA GACCAAGCCT CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC 
    TTCTTCACCC CCCGGAAGCC AGCAGTCAAA AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT 
    CCAGGAAAGG AGGGAGCTGC TGAGGGACCC CTGGATCCAT CTGGTTACAA TCCTGCCAAG 
    AACAACTATC ATCCCGTGGA AGATGCCTGC TGGAAACCGG GCCAGAAGGT TCCTTACCTG 
    GCTGTGGCCC GGACGTTTGA GAAGATCGAG GAGGTGTCTG CTCGGCTCCG GATGGTGGAG 
    ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG GCCCTGTCGC CTCCAGACCT CCTCCCTGTC 
    CTCTACCTCA GCCTCAACCA CCTTGGGCCA CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT 
    GATGGTGTCC TTCTCAAGGC AGTGGCCCAG GCCACAGGTC GGCAGCTGGA GTCCGTCCGG 
    GCTGAGGCAG CCGAGAAAGG CGACGTGGGG CTGGTGGCCG AGAACAGCCG CAGCACCCAG 
    AGGCTCATGC TGCCACCACC TCCGCTCACT GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC 
    ATCGCCAGGC TCACTGGCAG TGCTTCCACA GCCAAGAAGA TAGACATCAT CAAAGGCCTC 
    TTTGTGGCCT GCCGCCACTC AGAAGCCCGG TTCATCGCTA GGTCCCTGAG CGGACGGCTG 
    CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG GCTGCCCTCT CCCAGGCAGT GAGCCTCACG 
    CCCCCGGGCC AAGAATTCCC ACCAGCCATG GTGGATGCTG GGAAGGGCAA GACAGCAGAG 
    GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC ATGATCCTGA AGCAGACGTT CTGCGAGGTT 
    CCCGACCTGG ACCGAATTAT CCCCGTGCTG CTGGAGCACG GCCTGGAACG TCTCCCGGAG 
    CACTGCAAGC TGAGCCCAGG GATTCCCCTG AAACCAATGT TGGCCCATCC CACCCGGGGC 
    ATCAGCGAGG TCCTGAAACG CTTTGAGGAG GCAGCTTTCA CCTGCGAATA CAAATATGAC 
    GGGCAGAGGG CACAGATCCA CGCCCTGGAA GGCGGGGAGG TGAAGATCTT CAGCAGGAAT 
    CAGGAAGACA ACACTGGGAA GTACCCGGAC ATCATCAGCC GCATCCCCAA GATTAAACTC 
    CCATCGGTCA CATCCTTCAT CCTGGACACC GAAGCCGTGG CTTGGGACCG GGAAAAGAAG 
    CAGATCCAGC CATTCCAAGT GCTCACCACC CGCAAACGCA AGGAGGTGGA TGCGTCTGAG 
    ATCCAGGTGC AGGTGTGTTT GTACGCCTTC GACCTCATCT ACCTCAATGG AGAGTCCCTG 
    GTACGTGAGC CCCTTTCCCG GCGCCGGCAG CTGCTCCGGG AGAACTTTGT GGAGACAGAG 
    GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC ACCAAGGACA TCGAGCAGAT CGCCGAGTTC 
    CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG GGGCTGATGG TGAAGACCCT GGATGTTGAT 
    GCCACCTACG AGATCGCCAA GAGATCGCAC AACTGGCTCA AGCTGAAGAA GGACTACCTT 
    GATGGCGTGG GTGACACCCT GGACCTGGTG GTGATCGGCG CCTACCTGGG CCGGGGGAAG 
    CGGGCCGGCC GGTACGGGGG CTTCCTGCTG GCCTCCTACG ACGAGGACAG TGAGGAGCTG 
    CAGGCCATAT GCAAGCTTGG AACTGGCTTC AGTGATGAGG AGCTGGAGGA GCATCACCAG 
    AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA CGCCCTTACG TGCGGATAGA TGGCGCTGTG 
    ATTCCCGACC ACTGGCTGGA CCCCAGCGCT GTGTGGGAGG TGAAGTGCGC TGACCTCTCC 
    CTCTCTCCCA TCTACCCTGC TGCGCGGGGC CTGGTGGATA GTGACAAGGG CATCTCCCTT 
    CGCTTCCCTC GGTTTATTCG AGTCCGTGAA GACAAGCAGC CGGAGCAGGC CACCACCAGT 
    GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA AGTCAGATTC AGAACCAACA AGGCGAGGAC 
    TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307900.1
    CDS421..3090
    Translation

    Target ORF information:

    RefSeq Version NM_001320971.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001320971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAAGA GGAGACCCCG AAAGAAAGCC TCACAGAGGC TGAAGTGGCC 
    ACAGAGAAGG AAGGAGAAGA CGGGGACCAG CCCACCACGC CTCCCAAGCC CCTAAAGACC 
    TCCAAAGCAG AGACCCCGAC GGAAAGCGTT TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA 
    CTGCAGGAGG AGGAAGAGCA GACCAAGCCT CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC 
    TTCTTCACCC CCCGGAAGCC AGCAGTCAAA AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT 
    CCAGGAAAGG AGGGAGCTGC TGAGGGACCC CTGGATCCAT CTGGTTACAA TCCTGCCAAG 
    AACAACTATC ATCCCGTGGA AGATGCCTGC TGGAAACCGG GCCAGAAGGT TCCTTACCTG 
    GCTGTGGCCC GGACGTTTGA GAAGATCGAG GAGGTGTCTG CTCGGCTCCG GATGGTGGAG 
    ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG GCCCTGTCGC CTCCAGACCT CCTCCCTGTC 
    CTCTACCTCA GCCTCAACCA CCTTGGGCCA CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT 
    GATGGTGTCC TTCTCAAGGC AGTGGCCCAG GCCACAGGTC GGCAGCTGGA GTCCGTCCGG 
    GCTGAGGCAG CCGAGAAAGG CGACGTGGGG CTGGTGGCCG AGAACAGCCG CAGCACCCAG 
    AGGCTCATGC TGCCACCACC TCCGCTCACT GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC 
    ATCGCCAGGC TCACTGGCAG TGCTTCCACA GCCAAGAAGA TAGACATCAT CAAAGGCCTC 
    TTTGTGGCCT GCCGCCACTC AGAAGCCCGG TTCATCGCTA GGTCCCTGAG CGGACGGCTG 
    CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG GCTGCCCTCT CCCAGGCAGT GAGCCTCACG 
    CCCCCGGGCC AAGAATTCCC ACCAGCCATG GTGGATGCTG GGAAGGGCAA GACAGCAGAG 
    GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC ATGATCCTGA AGCAGACGTT CTGCGAGGTT 
    CCCGACCTGG ACCGAATTAT CCCCGTGCTG CTGGAGCACG GCCTGGAACG TCTCCCGGAG 
    CACTGCAAGC TGAGCCCAGG GATTCCCCTG AAACCAATGT TGGCCCATCC CACCCGGGGC 
    ATCAGCGAGG TCCTGAAACG CTTTGAGGAG GCAGCTTTCA CCTGCGAATA CAAATATGAC 
    GGGCAGAGGG CACAGATCCA CGCCCTGGAA GGCGGGGAGG TGAAGATCTT CAGCAGGAAT 
    CAGGAAGACA ACACTGGGAA GTACCCGGAC ATCATCAGCC GCATCCCCAA GATTAAACTC 
    CCATCGGTCA CATCCTTCAT CCTGGACACC GAAGCCGTGG CTTGGGACCG GGAAAAGAAG 
    CAGATCCAGC CATTCCAAGT GCTCACCACC CGCAAACGCA AGGAGGTGGA TGCGTCTGAG 
    ATCCAGGTGC AGGTGTGTTT GTACGCCTTC GACCTCATCT ACCTCAATGG AGAGTCCCTG 
    GTACGTGAGC CCCTTTCCCG GCGCCGGCAG CTGCTCCGGG AGAACTTTGT GGAGACAGAG 
    GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC ACCAAGGACA TCGAGCAGAT CGCCGAGTTC 
    CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG GGGCTGATGG TGAAGACCCT GGATGTTGAT 
    GCCACCTACG AGATCGCCAA GAGATCGCAC AACTGGCTCA AGCTGAAGAA GGACTACCTT 
    GATGGCGTGG GTGACACCCT GGACCTGGTG GTGATCGGCG CCTACCTGGG CCGGGGGAAG 
    CGGGCCGGCC GGTACGGGGG CTTCCTGCTG GCCTCCTACG ACGAGGACAG TGAGGAGCTG 
    CAGGCCATAT GCAAGCTTGG AACTGGCTTC AGTGATGAGG AGCTGGAGGA GCATCACCAG 
    AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA CGCCCTTACG TGCGGATAGA TGGCGCTGTG 
    ATTCCCGACC ACTGGCTGGA CCCCAGCGCT GTGTGGGAGG TGAAGTGCGC TGACCTCTCC 
    CTCTCTCCCA TCTACCCTGC TGCGCGGGGC CTGGTGGATA GTGACAAGGG CATCTCCCTT 
    CGCTTCCCTC GGTTTATTCG AGTCCGTGAA GACAAGCAGC CGGAGCAGGC CACCACCAGT 
    GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA AGTCAGATTC AGAACCAACA AGGCGAGGAC 
    TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( NM_001320971.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307900.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA.
    Price & Availability↑
    CloneID OHu52263
    Clone ID Related Accession (Same CDS sequence) NM_001320971.1 , NM_001320971.2
    Accession Version NM_001320971.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-25
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011466.6, KC877741.1 and BC108318.1. On May 31, 2019 this sequence version replaced NM_001320971.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.8621.1, SRR1660805.135014.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAAGA GGAGACCCCG AAAGAAAGCC TCACAGAGGC TGAAGTGGCC 
    ACAGAGAAGG AAGGAGAAGA CGGGGACCAG CCCACCACGC CTCCCAAGCC CCTAAAGACC 
    TCCAAAGCAG AGACCCCGAC GGAAAGCGTT TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA 
    CTGCAGGAGG AGGAAGAGCA GACCAAGCCT CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC 
    TTCTTCACCC CCCGGAAGCC AGCAGTCAAA AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT 
    CCAGGAAAGG AGGGAGCTGC TGAGGGACCC CTGGATCCAT CTGGTTACAA TCCTGCCAAG 
    AACAACTATC ATCCCGTGGA AGATGCCTGC TGGAAACCGG GCCAGAAGGT TCCTTACCTG 
    GCTGTGGCCC GGACGTTTGA GAAGATCGAG GAGGTGTCTG CTCGGCTCCG GATGGTGGAG 
    ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG GCCCTGTCGC CTCCAGACCT CCTCCCTGTC 
    CTCTACCTCA GCCTCAACCA CCTTGGGCCA CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT 
    GATGGTGTCC TTCTCAAGGC AGTGGCCCAG GCCACAGGTC GGCAGCTGGA GTCCGTCCGG 
    GCTGAGGCAG CCGAGAAAGG CGACGTGGGG CTGGTGGCCG AGAACAGCCG CAGCACCCAG 
    AGGCTCATGC TGCCACCACC TCCGCTCACT GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC 
    ATCGCCAGGC TCACTGGCAG TGCTTCCACA GCCAAGAAGA TAGACATCAT CAAAGGCCTC 
    TTTGTGGCCT GCCGCCACTC AGAAGCCCGG TTCATCGCTA GGTCCCTGAG CGGACGGCTG 
    CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG GCTGCCCTCT CCCAGGCAGT GAGCCTCACG 
    CCCCCGGGCC AAGAATTCCC ACCAGCCATG GTGGATGCTG GGAAGGGCAA GACAGCAGAG 
    GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC ATGATCCTGA AGCAGACGTT CTGCGAGGTT 
    CCCGACCTGG ACCGAATTAT CCCCGTGCTG CTGGAGCACG GCCTGGAACG TCTCCCGGAG 
    CACTGCAAGC TGAGCCCAGG GATTCCCCTG AAACCAATGT TGGCCCATCC CACCCGGGGC 
    ATCAGCGAGG TCCTGAAACG CTTTGAGGAG GCAGCTTTCA CCTGCGAATA CAAATATGAC 
    GGGCAGAGGG CACAGATCCA CGCCCTGGAA GGCGGGGAGG TGAAGATCTT CAGCAGGAAT 
    CAGGAAGACA ACACTGGGAA GTACCCGGAC ATCATCAGCC GCATCCCCAA GATTAAACTC 
    CCATCGGTCA CATCCTTCAT CCTGGACACC GAAGCCGTGG CTTGGGACCG GGAAAAGAAG 
    CAGATCCAGC CATTCCAAGT GCTCACCACC CGCAAACGCA AGGAGGTGGA TGCGTCTGAG 
    ATCCAGGTGC AGGTGTGTTT GTACGCCTTC GACCTCATCT ACCTCAATGG AGAGTCCCTG 
    GTACGTGAGC CCCTTTCCCG GCGCCGGCAG CTGCTCCGGG AGAACTTTGT GGAGACAGAG 
    GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC ACCAAGGACA TCGAGCAGAT CGCCGAGTTC 
    CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG GGGCTGATGG TGAAGACCCT GGATGTTGAT 
    GCCACCTACG AGATCGCCAA GAGATCGCAC AACTGGCTCA AGCTGAAGAA GGACTACCTT 
    GATGGCGTGG GTGACACCCT GGACCTGGTG GTGATCGGCG CCTACCTGGG CCGGGGGAAG 
    CGGGCCGGCC GGTACGGGGG CTTCCTGCTG GCCTCCTACG ACGAGGACAG TGAGGAGCTG 
    CAGGCCATAT GCAAGCTTGG AACTGGCTTC AGTGATGAGG AGCTGGAGGA GCATCACCAG 
    AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA CGCCCTTACG TGCGGATAGA TGGCGCTGTG 
    ATTCCCGACC ACTGGCTGGA CCCCAGCGCT GTGTGGGAGG TGAAGTGCGC TGACCTCTCC 
    CTCTCTCCCA TCTACCCTGC TGCGCGGGGC CTGGTGGATA GTGACAAGGG CATCTCCCTT 
    CGCTTCCCTC GGTTTATTCG AGTCCGTGAA GACAAGCAGC CGGAGCAGGC CACCACCAGT 
    GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA AGTCAGATTC AGAACCAACA AGGCGAGGAC 
    TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001307900.1
    CDS162..2831
    Translation

    Target ORF information:

    RefSeq Version NM_001320971.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001320971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGGC GGCACTGAAG GAGTGGAATG GAGTGGTGTC CGAGAGTGAC 
    TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG 
    GAGGATGAAG CCCTTAGCCC TGCTAAAGGC CAGAAGCCTG CCCTGGACTG CTCACAGGTC 
    TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG AACAATGCTT CCCTCTCTGA CACCTCTCCC 
    ATGGACAGTT CCCCATCAGG GATTCCGAAG CGTCGCACAG CTCGGAAGCA GCTCCCGAAA 
    CGGACCATTC AGGAAGTCCT GGAAGAGCAG AGTGAGGACG AGGACAGAGA AGCCAAGAGG 
    AAGAAGGAGG AGGAAGAAGA GGAGACCCCG AAAGAAAGCC TCACAGAGGC TGAAGTGGCC 
    ACAGAGAAGG AAGGAGAAGA CGGGGACCAG CCCACCACGC CTCCCAAGCC CCTAAAGACC 
    TCCAAAGCAG AGACCCCGAC GGAAAGCGTT TCAGAGCCTG AGGTGGCCAC GAAGCAGGAA 
    CTGCAGGAGG AGGAAGAGCA GACCAAGCCT CCCCGCAGAG CTCCCAAGAC GCTCAGCAGC 
    TTCTTCACCC CCCGGAAGCC AGCAGTCAAA AAAGAAGTGA AGGAAGAGGA GCCAGGGGCT 
    CCAGGAAAGG AGGGAGCTGC TGAGGGACCC CTGGATCCAT CTGGTTACAA TCCTGCCAAG 
    AACAACTATC ATCCCGTGGA AGATGCCTGC TGGAAACCGG GCCAGAAGGT TCCTTACCTG 
    GCTGTGGCCC GGACGTTTGA GAAGATCGAG GAGGTGTCTG CTCGGCTCCG GATGGTGGAG 
    ACGCTGAGCA ACTTGCTGCG CTCCGTGGTG GCCCTGTCGC CTCCAGACCT CCTCCCTGTC 
    CTCTACCTCA GCCTCAACCA CCTTGGGCCA CCCCAGCAGG GCCTGGAGCT TGGCGTGGGT 
    GATGGTGTCC TTCTCAAGGC AGTGGCCCAG GCCACAGGTC GGCAGCTGGA GTCCGTCCGG 
    GCTGAGGCAG CCGAGAAAGG CGACGTGGGG CTGGTGGCCG AGAACAGCCG CAGCACCCAG 
    AGGCTCATGC TGCCACCACC TCCGCTCACT GCCTCCGGGG TCTTCAGCAA GTTCCGCGAC 
    ATCGCCAGGC TCACTGGCAG TGCTTCCACA GCCAAGAAGA TAGACATCAT CAAAGGCCTC 
    TTTGTGGCCT GCCGCCACTC AGAAGCCCGG TTCATCGCTA GGTCCCTGAG CGGACGGCTG 
    CGCCTTGGGC TGGCAGAGCA GTCGGTGCTG GCTGCCCTCT CCCAGGCAGT GAGCCTCACG 
    CCCCCGGGCC AAGAATTCCC ACCAGCCATG GTGGATGCTG GGAAGGGCAA GACAGCAGAG 
    GCCAGAAAGA CGTGGCTGGA GGAGCAAGGC ATGATCCTGA AGCAGACGTT CTGCGAGGTT 
    CCCGACCTGG ACCGAATTAT CCCCGTGCTG CTGGAGCACG GCCTGGAACG TCTCCCGGAG 
    CACTGCAAGC TGAGCCCAGG GATTCCCCTG AAACCAATGT TGGCCCATCC CACCCGGGGC 
    ATCAGCGAGG TCCTGAAACG CTTTGAGGAG GCAGCTTTCA CCTGCGAATA CAAATATGAC 
    GGGCAGAGGG CACAGATCCA CGCCCTGGAA GGCGGGGAGG TGAAGATCTT CAGCAGGAAT 
    CAGGAAGACA ACACTGGGAA GTACCCGGAC ATCATCAGCC GCATCCCCAA GATTAAACTC 
    CCATCGGTCA CATCCTTCAT CCTGGACACC GAAGCCGTGG CTTGGGACCG GGAAAAGAAG 
    CAGATCCAGC CATTCCAAGT GCTCACCACC CGCAAACGCA AGGAGGTGGA TGCGTCTGAG 
    ATCCAGGTGC AGGTGTGTTT GTACGCCTTC GACCTCATCT ACCTCAATGG AGAGTCCCTG 
    GTACGTGAGC CCCTTTCCCG GCGCCGGCAG CTGCTCCGGG AGAACTTTGT GGAGACAGAG 
    GGCGAGTTTG TCTTCGCCAC CTCCCTGGAC ACCAAGGACA TCGAGCAGAT CGCCGAGTTC 
    CTGGAGCAGT CAGTGAAAGA CTCCTGCGAG GGGCTGATGG TGAAGACCCT GGATGTTGAT 
    GCCACCTACG AGATCGCCAA GAGATCGCAC AACTGGCTCA AGCTGAAGAA GGACTACCTT 
    GATGGCGTGG GTGACACCCT GGACCTGGTG GTGATCGGCG CCTACCTGGG CCGGGGGAAG 
    CGGGCCGGCC GGTACGGGGG CTTCCTGCTG GCCTCCTACG ACGAGGACAG TGAGGAGCTG 
    CAGGCCATAT GCAAGCTTGG AACTGGCTTC AGTGATGAGG AGCTGGAGGA GCATCACCAG 
    AGCCTCAAGG CGCTGGTGCT GCCCAGCCCA CGCCCTTACG TGCGGATAGA TGGCGCTGTG 
    ATTCCCGACC ACTGGCTGGA CCCCAGCGCT GTGTGGGAGG TGAAGTGCGC TGACCTCTCC 
    CTCTCTCCCA TCTACCCTGC TGCGCGGGGC CTGGTGGATA GTGACAAGGG CATCTCCCTT 
    CGCTTCCCTC GGTTTATTCG AGTCCGTGAA GACAAGCAGC CGGAGCAGGC CACCACCAGT 
    GCTCAGGTGG CCTGTTTGTA CCGGAAGCAA AGTCAGATTC AGAACCAACA AGGCGAGGAC 
    TCAGGCTCTG ACCCTGAAGA TACCTACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( XM_017026809.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882298.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu88469
    Clone ID Related Accession (Same CDS sequence) XM_017026809.1 , XM_024451513.1
    Accession Version XM_017026809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGC AGAGACCCCG 
    ACGGAAAGCG TTTCAGAGCC TGAGGTGGCC ACGAAGCAGG AACTGCAGGA GGAGGAAGAG 
    CAGACCAAGC CTCCCCGCAG AGCTCCCAAG ACGCTCAGCA GCTTCTTCAC CCCCCGGAAG 
    CCAGCAGTCA AAAAAGAAGT GAAGGAAGAG GAGCCAGGGG CTCCAGGAAA GGAGGGAGCT 
    GCTGAGGGAC CCCTGGATCC ATCTGGTTAC AATCCTGCCA AGAACAACTA TCATCCCGTG 
    GAAGATGCCT GCTGGAAACC GGGCCAGAAG GTTCCTTACC TGGCTGTGGC CCGGACGTTT 
    GAGAAGATCG AGGAGGTGTC TGCTCGGCTC CGGATGGTGG AGACGCTGAG CAACTTGCTG 
    CGCTCCGTGG TGGCCCTGTC GCCTCCAGAC CTCCTCCCTG TCCTCTACCT CAGCCTCAAC 
    CACCTTGGGC CACCCCAGCA GGGCCTGGAG CTTGGCGTGG GTGATGGTGT CCTTCTCAAG 
    GCAGTGGCCC AGGCCACAGG TCGGCAGCTG GAGTCCGTCC GGGCTGAGGC AGCCGAGAAA 
    GGCGACGTGG GGCTGGTGGC CGAGAACAGC CGCAGCACCC AGAGGCTCAT GCTGCCACCA 
    CCTCCGCTCA CTGCCTCCGG GGTCTTCAGC AAGTTCCGCG ACATCGCCAG GCTCACTGGC 
    AGTGCTTCCA CAGCCAAGAA GATAGACATC ATCAAAGGCC TCTTTGTGGC CTGCCGCCAC 
    TCAGAAGCCC GGTTCATCGC TAGGTCCCTG AGCGGACGGC TGCGCCTTGG GCTGGCAGAG 
    CAGTCGGTGC TGGCTGCCCT CTCCCAGGCA GTGAGCCTCA CGCCCCCGGG CCAAGAATTC 
    CCACCAGCCA TGGTGGATGC TGGGAAGGGC AAGACAGCAG AGGCCAGAAA GACGTGGCTG 
    GAGGAGCAAG GCATGATCCT GAAGCAGACG TTCTGCGAGG TTCCCGACCT GGACCGAATT 
    ATCCCCGTGC TGCTGGAGCA CGGCCTGGAA CGTCTCCCGG AGCACTGCAA GCTGAGCCCA 
    GGGATTCCCC TGAAACCAAT GTTGGCCCAT CCCACCCGGG GCATCAGCGA GGTCCTGAAA 
    CGCTTTGAGG AGGCAGCTTT CACCTGCGAA TACAAATATG ACGGGCAGAG GGCACAGATC 
    CACGCCCTGG AAGGCGGGGA GGTGAAGATC TTCAGCAGGA ATCAGGAAGA CAACACTGGG 
    AAGTACCCGG ACATCATCAG CCGCATCCCC AAGATTAAAC TCCCATCGGT CACATCCTTC 
    ATCCTGGACA CCGAAGCCGT GGCTTGGGAC CGGGAAAAGA AGCAGATCCA GCCATTCCAA 
    GTGCTCACCA CCCGCAAACG CAAGGAGGTG GATGCGTCTG AGATCCAGGT GCAGGTGTGT 
    TTGTACGCCT TCGACCTCAT CTACCTCAAT GGAGAGTCCC TGGTACGTGA GCCCCTTTCC 
    CGGCGCCGGC AGCTGCTCCG GGAGAACTTT GTGGAGACAG AGGGCGAGTT TGTCTTCGCC 
    ACCTCCCTGG ACACCAAGGA CATCGAGCAG ATCGCCGAGT TCCTGGAGCA GTCAGTGAAA 
    GACTCCTGCG AGGGGCTGAT GGTGAAGACC CTGGATGTTG ATGCCACCTA CGAGATCGCC 
    AAGAGATCGC ACAACTGGCT CAAGCTGAAG AAGGACTACC TTGATGGCGT GGGTGACACC 
    CTGGACCTGG TGGTGATCGG CGCCTACCTG GGCCGGGGGA AGCGGGCCGG CCGGTACGGG 
    GGCTTCCTGC TGGCCTCCTA CGACGAGGAC AGTGAGGAGC TGCAGGCCAT ATGCAAGCTT 
    GGAACTGGCT TCAGTGATGA GGAGCTGGAG GAGCATCACC AGAGCCTCAA GGCGCTGGTG 
    CTGCCCAGCC CACGCCCTTA CGTGCGGATA GATGGCGCTG TGATTCCCGA CCACTGGCTG 
    GACCCCAGCG CTGTGTGGGA GGTGAAGTGC GCTGACCTCT CCCTCTCTCC CATCTACCCT 
    GCTGCGCGGG GCCTGGTGGA TAGTGACAAG GGCATCTCCC TTCGCTTCCC TCGGTTTATT 
    CGAGTCCGTG AAGACAAGCA GCCGGAGCAG GCCACCACCA GTGCTCAGGT GGCCTGTTTG 
    TACCGGAAGC AAAGTCAGAT TCAGAACCAA CAAGGCGAGG ACTCAGGCTC TGACCCTGAA 
    GATACCTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882298.1
    CDS58..2709
    Translation

    Target ORF information:

    RefSeq Version XM_017026809.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017026809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGC AGAGACCCCG 
    ACGGAAAGCG TTTCAGAGCC TGAGGTGGCC ACGAAGCAGG AACTGCAGGA GGAGGAAGAG 
    CAGACCAAGC CTCCCCGCAG AGCTCCCAAG ACGCTCAGCA GCTTCTTCAC CCCCCGGAAG 
    CCAGCAGTCA AAAAAGAAGT GAAGGAAGAG GAGCCAGGGG CTCCAGGAAA GGAGGGAGCT 
    GCTGAGGGAC CCCTGGATCC ATCTGGTTAC AATCCTGCCA AGAACAACTA TCATCCCGTG 
    GAAGATGCCT GCTGGAAACC GGGCCAGAAG GTTCCTTACC TGGCTGTGGC CCGGACGTTT 
    GAGAAGATCG AGGAGGTGTC TGCTCGGCTC CGGATGGTGG AGACGCTGAG CAACTTGCTG 
    CGCTCCGTGG TGGCCCTGTC GCCTCCAGAC CTCCTCCCTG TCCTCTACCT CAGCCTCAAC 
    CACCTTGGGC CACCCCAGCA GGGCCTGGAG CTTGGCGTGG GTGATGGTGT CCTTCTCAAG 
    GCAGTGGCCC AGGCCACAGG TCGGCAGCTG GAGTCCGTCC GGGCTGAGGC AGCCGAGAAA 
    GGCGACGTGG GGCTGGTGGC CGAGAACAGC CGCAGCACCC AGAGGCTCAT GCTGCCACCA 
    CCTCCGCTCA CTGCCTCCGG GGTCTTCAGC AAGTTCCGCG ACATCGCCAG GCTCACTGGC 
    AGTGCTTCCA CAGCCAAGAA GATAGACATC ATCAAAGGCC TCTTTGTGGC CTGCCGCCAC 
    TCAGAAGCCC GGTTCATCGC TAGGTCCCTG AGCGGACGGC TGCGCCTTGG GCTGGCAGAG 
    CAGTCGGTGC TGGCTGCCCT CTCCCAGGCA GTGAGCCTCA CGCCCCCGGG CCAAGAATTC 
    CCACCAGCCA TGGTGGATGC TGGGAAGGGC AAGACAGCAG AGGCCAGAAA GACGTGGCTG 
    GAGGAGCAAG GCATGATCCT GAAGCAGACG TTCTGCGAGG TTCCCGACCT GGACCGAATT 
    ATCCCCGTGC TGCTGGAGCA CGGCCTGGAA CGTCTCCCGG AGCACTGCAA GCTGAGCCCA 
    GGGATTCCCC TGAAACCAAT GTTGGCCCAT CCCACCCGGG GCATCAGCGA GGTCCTGAAA 
    CGCTTTGAGG AGGCAGCTTT CACCTGCGAA TACAAATATG ACGGGCAGAG GGCACAGATC 
    CACGCCCTGG AAGGCGGGGA GGTGAAGATC TTCAGCAGGA ATCAGGAAGA CAACACTGGG 
    AAGTACCCGG ACATCATCAG CCGCATCCCC AAGATTAAAC TCCCATCGGT CACATCCTTC 
    ATCCTGGACA CCGAAGCCGT GGCTTGGGAC CGGGAAAAGA AGCAGATCCA GCCATTCCAA 
    GTGCTCACCA CCCGCAAACG CAAGGAGGTG GATGCGTCTG AGATCCAGGT GCAGGTGTGT 
    TTGTACGCCT TCGACCTCAT CTACCTCAAT GGAGAGTCCC TGGTACGTGA GCCCCTTTCC 
    CGGCGCCGGC AGCTGCTCCG GGAGAACTTT GTGGAGACAG AGGGCGAGTT TGTCTTCGCC 
    ACCTCCCTGG ACACCAAGGA CATCGAGCAG ATCGCCGAGT TCCTGGAGCA GTCAGTGAAA 
    GACTCCTGCG AGGGGCTGAT GGTGAAGACC CTGGATGTTG ATGCCACCTA CGAGATCGCC 
    AAGAGATCGC ACAACTGGCT CAAGCTGAAG AAGGACTACC TTGATGGCGT GGGTGACACC 
    CTGGACCTGG TGGTGATCGG CGCCTACCTG GGCCGGGGGA AGCGGGCCGG CCGGTACGGG 
    GGCTTCCTGC TGGCCTCCTA CGACGAGGAC AGTGAGGAGC TGCAGGCCAT ATGCAAGCTT 
    GGAACTGGCT TCAGTGATGA GGAGCTGGAG GAGCATCACC AGAGCCTCAA GGCGCTGGTG 
    CTGCCCAGCC CACGCCCTTA CGTGCGGATA GATGGCGCTG TGATTCCCGA CCACTGGCTG 
    GACCCCAGCG CTGTGTGGGA GGTGAAGTGC GCTGACCTCT CCCTCTCTCC CATCTACCCT 
    GCTGCGCGGG GCCTGGTGGA TAGTGACAAG GGCATCTCCC TTCGCTTCCC TCGGTTTATT 
    CGAGTCCGTG AAGACAAGCA GCCGGAGCAG GCCACCACCA GTGCTCAGGT GGCCTGTTTG 
    TACCGGAAGC AAAGTCAGAT TCAGAACCAA CAAGGCGAGG ACTCAGGCTC TGACCCTGAA 
    GATACCTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LIG1 ( XM_024451513.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307281.1 Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OHu88469
    Clone ID Related Accession (Same CDS sequence) XM_017026809.1 , XM_024451513.1
    Accession Version XM_024451513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product DNA ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGC AGAGACCCCG 
    ACGGAAAGCG TTTCAGAGCC TGAGGTGGCC ACGAAGCAGG AACTGCAGGA GGAGGAAGAG 
    CAGACCAAGC CTCCCCGCAG AGCTCCCAAG ACGCTCAGCA GCTTCTTCAC CCCCCGGAAG 
    CCAGCAGTCA AAAAAGAAGT GAAGGAAGAG GAGCCAGGGG CTCCAGGAAA GGAGGGAGCT 
    GCTGAGGGAC CCCTGGATCC ATCTGGTTAC AATCCTGCCA AGAACAACTA TCATCCCGTG 
    GAAGATGCCT GCTGGAAACC GGGCCAGAAG GTTCCTTACC TGGCTGTGGC CCGGACGTTT 
    GAGAAGATCG AGGAGGTGTC TGCTCGGCTC CGGATGGTGG AGACGCTGAG CAACTTGCTG 
    CGCTCCGTGG TGGCCCTGTC GCCTCCAGAC CTCCTCCCTG TCCTCTACCT CAGCCTCAAC 
    CACCTTGGGC CACCCCAGCA GGGCCTGGAG CTTGGCGTGG GTGATGGTGT CCTTCTCAAG 
    GCAGTGGCCC AGGCCACAGG TCGGCAGCTG GAGTCCGTCC GGGCTGAGGC AGCCGAGAAA 
    GGCGACGTGG GGCTGGTGGC CGAGAACAGC CGCAGCACCC AGAGGCTCAT GCTGCCACCA 
    CCTCCGCTCA CTGCCTCCGG GGTCTTCAGC AAGTTCCGCG ACATCGCCAG GCTCACTGGC 
    AGTGCTTCCA CAGCCAAGAA GATAGACATC ATCAAAGGCC TCTTTGTGGC CTGCCGCCAC 
    TCAGAAGCCC GGTTCATCGC TAGGTCCCTG AGCGGACGGC TGCGCCTTGG GCTGGCAGAG 
    CAGTCGGTGC TGGCTGCCCT CTCCCAGGCA GTGAGCCTCA CGCCCCCGGG CCAAGAATTC 
    CCACCAGCCA TGGTGGATGC TGGGAAGGGC AAGACAGCAG AGGCCAGAAA GACGTGGCTG 
    GAGGAGCAAG GCATGATCCT GAAGCAGACG TTCTGCGAGG TTCCCGACCT GGACCGAATT 
    ATCCCCGTGC TGCTGGAGCA CGGCCTGGAA CGTCTCCCGG AGCACTGCAA GCTGAGCCCA 
    GGGATTCCCC TGAAACCAAT GTTGGCCCAT CCCACCCGGG GCATCAGCGA GGTCCTGAAA 
    CGCTTTGAGG AGGCAGCTTT CACCTGCGAA TACAAATATG ACGGGCAGAG GGCACAGATC 
    CACGCCCTGG AAGGCGGGGA GGTGAAGATC TTCAGCAGGA ATCAGGAAGA CAACACTGGG 
    AAGTACCCGG ACATCATCAG CCGCATCCCC AAGATTAAAC TCCCATCGGT CACATCCTTC 
    ATCCTGGACA CCGAAGCCGT GGCTTGGGAC CGGGAAAAGA AGCAGATCCA GCCATTCCAA 
    GTGCTCACCA CCCGCAAACG CAAGGAGGTG GATGCGTCTG AGATCCAGGT GCAGGTGTGT 
    TTGTACGCCT TCGACCTCAT CTACCTCAAT GGAGAGTCCC TGGTACGTGA GCCCCTTTCC 
    CGGCGCCGGC AGCTGCTCCG GGAGAACTTT GTGGAGACAG AGGGCGAGTT TGTCTTCGCC 
    ACCTCCCTGG ACACCAAGGA CATCGAGCAG ATCGCCGAGT TCCTGGAGCA GTCAGTGAAA 
    GACTCCTGCG AGGGGCTGAT GGTGAAGACC CTGGATGTTG ATGCCACCTA CGAGATCGCC 
    AAGAGATCGC ACAACTGGCT CAAGCTGAAG AAGGACTACC TTGATGGCGT GGGTGACACC 
    CTGGACCTGG TGGTGATCGG CGCCTACCTG GGCCGGGGGA AGCGGGCCGG CCGGTACGGG 
    GGCTTCCTGC TGGCCTCCTA CGACGAGGAC AGTGAGGAGC TGCAGGCCAT ATGCAAGCTT 
    GGAACTGGCT TCAGTGATGA GGAGCTGGAG GAGCATCACC AGAGCCTCAA GGCGCTGGTG 
    CTGCCCAGCC CACGCCCTTA CGTGCGGATA GATGGCGCTG TGATTCCCGA CCACTGGCTG 
    GACCCCAGCG CTGTGTGGGA GGTGAAGTGC GCTGACCTCT CCCTCTCTCC CATCTACCCT 
    GCTGCGCGGG GCCTGGTGGA TAGTGACAAG GGCATCTCCC TTCGCTTCCC TCGGTTTATT 
    CGAGTCCGTG AAGACAAGCA GCCGGAGCAG GCCACCACCA GTGCTCAGGT GGCCTGTTTG 
    TACCGGAAGC AAAGTCAGAT TCAGAACCAA CAAGGCGAGG ACTCAGGCTC TGACCCTGAA 
    GATACCTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024307281.1
    CDS413..3064
    Translation

    Target ORF information:

    RefSeq Version XM_024451513.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 1 (LIG1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024451513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCAGCGAA GTATCATGTC ATTTTTCCAC CCCAAGAAAG AGGGTAAAGC AAAGAAGCCT 
    GAGAAGGAGG CATCCAATAG CAGCAGAGAG ACGGAGCCCC CTCCAAAGGC GGCACTGAAG 
    GAGTGGAATG GAGTGGTGTC CGAGAGTGAC TCTCCGGTGA AGAGGCCAGG GAGGAAGGCG 
    GCCCGGGTCC TGGGCAGCGA AGGGGAAGAG GAGGATGAAG CCCTTAGCCC TGCTAAAGGC 
    CAGAAGCCTG CCCTGGACTG CTCACAGGTC TCCCCGCCCC GTCCTGCCAC ATCTCCTGAG 
    AACAATGCTT CCCTCTCTGA CACCTCTCCC ATGGACAGTT CCCCATCAGG GATTCCGAAG 
    CGTCGCACAG CTCGGAAGCA GCTCCCGAAA CGGACCATTC AGGAAGTCCT GGAAGAGCAG 
    AGTGAGGACG AGGACAGAGA AGCCAAGAGG AAGAAGGAGG AGGAAGAAGC AGAGACCCCG 
    ACGGAAAGCG TTTCAGAGCC TGAGGTGGCC ACGAAGCAGG AACTGCAGGA GGAGGAAGAG 
    CAGACCAAGC CTCCCCGCAG AGCTCCCAAG ACGCTCAGCA GCTTCTTCAC CCCCCGGAAG 
    CCAGCAGTCA AAAAAGAAGT GAAGGAAGAG GAGCCAGGGG CTCCAGGAAA GGAGGGAGCT 
    GCTGAGGGAC CCCTGGATCC ATCTGGTTAC AATCCTGCCA AGAACAACTA TCATCCCGTG 
    GAAGATGCCT GCTGGAAACC GGGCCAGAAG GTTCCTTACC TGGCTGTGGC CCGGACGTTT 
    GAGAAGATCG AGGAGGTGTC TGCTCGGCTC CGGATGGTGG AGACGCTGAG CAACTTGCTG 
    CGCTCCGTGG TGGCCCTGTC GCCTCCAGAC CTCCTCCCTG TCCTCTACCT CAGCCTCAAC 
    CACCTTGGGC CACCCCAGCA GGGCCTGGAG CTTGGCGTGG GTGATGGTGT CCTTCTCAAG 
    GCAGTGGCCC AGGCCACAGG TCGGCAGCTG GAGTCCGTCC GGGCTGAGGC AGCCGAGAAA 
    GGCGACGTGG GGCTGGTGGC CGAGAACAGC CGCAGCACCC AGAGGCTCAT GCTGCCACCA 
    CCTCCGCTCA CTGCCTCCGG GGTCTTCAGC AAGTTCCGCG ACATCGCCAG GCTCACTGGC 
    AGTGCTTCCA CAGCCAAGAA GATAGACATC ATCAAAGGCC TCTTTGTGGC CTGCCGCCAC 
    TCAGAAGCCC GGTTCATCGC TAGGTCCCTG AGCGGACGGC TGCGCCTTGG GCTGGCAGAG 
    CAGTCGGTGC TGGCTGCCCT CTCCCAGGCA GTGAGCCTCA CGCCCCCGGG CCAAGAATTC 
    CCACCAGCCA TGGTGGATGC TGGGAAGGGC AAGACAGCAG AGGCCAGAAA GACGTGGCTG 
    GAGGAGCAAG GCATGATCCT GAAGCAGACG TTCTGCGAGG TTCCCGACCT GGACCGAATT 
    ATCCCCGTGC TGCTGGAGCA CGGCCTGGAA CGTCTCCCGG AGCACTGCAA GCTGAGCCCA 
    GGGATTCCCC TGAAACCAAT GTTGGCCCAT CCCACCCGGG GCATCAGCGA GGTCCTGAAA 
    CGCTTTGAGG AGGCAGCTTT CACCTGCGAA TACAAATATG ACGGGCAGAG GGCACAGATC 
    CACGCCCTGG AAGGCGGGGA GGTGAAGATC TTCAGCAGGA ATCAGGAAGA CAACACTGGG 
    AAGTACCCGG ACATCATCAG CCGCATCCCC AAGATTAAAC TCCCATCGGT CACATCCTTC 
    ATCCTGGACA CCGAAGCCGT GGCTTGGGAC CGGGAAAAGA AGCAGATCCA GCCATTCCAA 
    GTGCTCACCA CCCGCAAACG CAAGGAGGTG GATGCGTCTG AGATCCAGGT GCAGGTGTGT 
    TTGTACGCCT TCGACCTCAT CTACCTCAAT GGAGAGTCCC TGGTACGTGA GCCCCTTTCC 
    CGGCGCCGGC AGCTGCTCCG GGAGAACTTT GTGGAGACAG AGGGCGAGTT TGTCTTCGCC 
    ACCTCCCTGG ACACCAAGGA CATCGAGCAG ATCGCCGAGT TCCTGGAGCA GTCAGTGAAA 
    GACTCCTGCG AGGGGCTGAT GGTGAAGACC CTGGATGTTG ATGCCACCTA CGAGATCGCC 
    AAGAGATCGC ACAACTGGCT CAAGCTGAAG AAGGACTACC TTGATGGCGT GGGTGACACC 
    CTGGACCTGG TGGTGATCGG CGCCTACCTG GGCCGGGGGA AGCGGGCCGG CCGGTACGGG 
    GGCTTCCTGC TGGCCTCCTA CGACGAGGAC AGTGAGGAGC TGCAGGCCAT ATGCAAGCTT 
    GGAACTGGCT TCAGTGATGA GGAGCTGGAG GAGCATCACC AGAGCCTCAA GGCGCTGGTG 
    CTGCCCAGCC CACGCCCTTA CGTGCGGATA GATGGCGCTG TGATTCCCGA CCACTGGCTG 
    GACCCCAGCG CTGTGTGGGA GGTGAAGTGC GCTGACCTCT CCCTCTCTCC CATCTACCCT 
    GCTGCGCGGG GCCTGGTGGA TAGTGACAAG GGCATCTCCC TTCGCTTCCC TCGGTTTATT 
    CGAGTCCGTG AAGACAAGCA GCCGGAGCAG GCCACCACCA GTGCTCAGGT GGCCTGTTTG 
    TACCGGAAGC AAAGTCAGAT TCAGAACCAA CAAGGCGAGG ACTCAGGCTC TGACCCTGAA 
    GATACCTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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