EIN2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIN2
Entrez Gene ID	831889
Full Name	NRAMP metal ion transporter family protein
Synonyms	ATEIN2,CKR1,CYTOKININ RESISTANT 1,ENHANCED RESPONSE TO ABA3,ERA3,ETHYLENE INSENSITIVE 2,F12E4.10,F12E4_10,ORE2,ORE3,ORESARA 2,ORESARA 3,PIR2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_120406.5	NP_195948.1	NRAMP metal ion transporter family protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that root responses to 6-pentyl-2H-pyran-2-one involve components of auxin transport and signaling and the ethylene-response modulator EIN2.
Title: The volatile 6-pentyl-2H-pyran-2-one from Trichoderma atroviride regulates Arabidopsis thaliana root morphogenesis via auxin signaling and ETHYLENE INSENSITIVE 2 functioning.

Mitogen-activated protein kinase 6 (MPK6) promoted C-terminal end of ORE3/EIN2 (CEND cleavage and nuclear translocation. Nuclear CEND accumulated ETHYLENE INSENSITIVE3 (EIN3), a transcription factor that accelerates MeJA-induced leaf senescence.
Title: Mitogen-activated protein kinase 6 mediates nuclear translocation of ORE3 to promote ORE9 gene expression in methyl jasmonate-induced leaf senescence.

Characterization of one of the targets of the translation regulatory machinery, the ethylene signaling component EBF2, indicates that the signaling molecule EIN2 and the nonsense-mediated decay proteins UPFs play a central role in this ethylene-induced translational response.
Title: Gene-specific translation regulation mediated by the hormone-signaling molecule EIN2.

Study reports another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA and find that the EBF1/2 3' UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3' UTRs.
Title: EIN2-directed translational regulation of ethylene signaling in Arabidopsis.

role in regulating organ growth by restricting cell expansion
Title: The Arabidopsis EIN2 restricts organ growth by retarding cell expansion.

The following EIN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb18932	NM_120406.5 Latest version!	Arabidopsis thaliana NRAMP metal ion transporter family protein (EIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIN2 ( NM_120406.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195948.1 Arabidopsis thaliana NRAMP metal ion transporter family protein (EIN2), mRNA.

Price & Availability↑

CloneID	OAb18932
Clone ID Related Accession (Same CDS sequence)	NM_120406.5
Accession Version	NM_120406.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NRAMP metal ion transporter family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_120406.4.

ATGGAAGCTG AAATTGTGAA TGTGAGACCT CAGCTAGGGT TTATCCAGAG AATGGTTCCT 
GCTCTACTTC CTGTCCTTTT GGTTTCTGTC GGATATATTG ATCCCGGGAA ATGGGTTGCA 
AATATCGAAG GAGGTGCTCG TTTCGGGTAT GACTTGGTGG CAATTACTCT GCTTTTCAAT 
TTTGCCGCCA TCTTATGCCA ATATGTTGCA GCTCGCATAA GCGTTGTGAC TGGTAAACAC 
TTGGCTCAGA TCTGCAATGA AGAATATGAC AAGTGGACGT GCATGTTCTT GGGCATTCAG 
GCGGAGTTCT CAGCAATTCT GCTCGACCTT ACCATGGTTG TGGGAGTTGC GCATGCACTT 
AACCTTTTGT TTGGGGTGGA GTTATCCACT GGAGTGTTTT TGGCCGCCAT GGATGCGTTT 
TTATTTCCTG TTTTCGCCTC TTTCCTTGAA AATGGTATGG CAAATACAGT ATCCATTTAC 
TCTGCAGGCC TGGTATTACT TCTCTATGTA TCTGGCGTCT TGCTGAGTCA GTCTGAGATC 
CCACTCTCTA TGAATGGAGT GTTAACTCGG TTAAATGGAG AGAGCGCATT CGCACTGATG 
GGTCTTCTTG GCGCAAGCAT CGTCCCTCAC AATTTTTATA TCCATTCTTA TTTTGCTGGG 
GAAAGTACAT CTTCGTCTGA TGTCGACAAG AGCAGCTTGT GTCAAGACCA TTTGTTCGCC 
ATCTTTGGTG TCTTCAGCGG ACTGTCACTT GTAAATTATG TATTGATGAA TGCAGCAGCT 
AATGTGTTTC ACAGTACTGG CCTTGTGGTA CTGACTTTTC ACGATGCCTT GTCACTAATG 
GAGCAGGTAT TTATGAGTCC GCTCATTCCA GTGGTCTTTT TGATGCTCTT GTTCTTCTCT 
AGTCAAATTA CCGCACTAGC TTGGGCTTTC GGTGGAGAGG TCGTCCTGCA TGACTTCCTG 
AAGATAGAAA TACCCGCTTG GCTTCATCGT GCTACAATCA GAATTCTTGC AGTTGCTCCT 
GCGCTTTATT GTGTATGGAC ATCTGGTGCA GACGGAATAT ACCAGTTACT TATATTCACC 
CAGGTCTTGG TGGCAATGAT GCTTCCTTGC TCGGTAATAC CGCTTTTCCG CATTGCTTCG 
TCGAGACAAA TCATGGGTGT CCATAAAATC CCTCAGGTTG GCGAGTTCCT CGCACTTACA 
ACGTTTTTGG GATTTCTGGG GTTGAATGTT GTTTTTGTTG TTGAGATGGT ATTTGGGAGC 
AGTGACTGGG CTGGTGGTTT GAGATGGAAT ACCGTGATGG GCACCTCGAT TCAGTACACC 
ACTCTGCTTG TATCGTCATG TGCATCCTTA TGCCTGATAC TCTGGCTGGC AGCCACGCCG 
CTGAAATCTG CGAGTAACAG AGCGGAAGCT CAAATATGGA ACATGGATGC TCAAAATGCT 
TTATCTTATC CATCTGTTCA AGAAGAGGAA ATTGAAAGAA CAGAAACAAG GAGGAACGAA 
GACGAATCAA TAGTGCGGTT GGAAAGCAGG GTAAAGGATC AGTTGGATAC TACGTCTGTT 
ACTAGCTCGG TCTATGATTT GCCAGAGAAC ATTCTAATGA CGGATCAAGA AATCCGTTCG 
AGCCCTCCAG AGGAAAGAGA GTTGGATGTA AAGTACTCTA CCTCTCAAGT TAGTAGTCTT 
AAGGAAGACT CTGATGTAAA GGAACAGTCT GTATTGCAGT CAACAGTGGT TAATGAGGTC 
AGTGATAAGG ATCTGATTGT TGAAACAAAG ATGGCGAAAA TTGAACCAAT GAGTCCTGTG 
GAGAAGATTG TTAGCATGGA GAATAACAGC AAGTTTATTG AAAAGGATGT TGAAGGGGTT 
TCATGGGAAA CAGAAGAAGC TACCAAAGCT GCTCCTACAA GCAACTTTAC TGTCGGATCT 
GATGGTCCTC CTTCATTCCG CAGCTTAAGT GGGGAAGGGG GAAGTGGGAC TGGAAGCCTT 
TCACGGTTGC AAGGTTTGGG ACGTGCTGCC CGGAGACACT TATCTGCGAT CCTTGATGAA 
TTTTGGGGAC ATTTATATGA TTTTCATGGG CAATTGGTTG CTGAAGCCAG GGCAAAGAAA 
CTAGATCAGC TGTTTGGCAC TGATCAAAAG TCAGCCTCTT CTATGAAAGC AGATTCGTTT 
GGAAAAGACA TTAGCAGTGG ATATTGCATG TCACCAACTG CGAAGGGAAT GGATTCACAG 
ATGACTTCAA GTTTATATGA TTCACTGAAG CAGCAGAGGA CACCGGGAAG TATCGATTCG 
TTGTATGGAT TACAAAGAGG TTCGTCACCG TCACCGTTGG TCAACCGTAT GCAGATGTTG 
GGTGCATATG GTAACACCAC TAATAATAAT AATGCTTACG AATTGAGTGA GAGAAGATAC 
TCTAGCCTGC GTGCTCCATC ATCTTCAGAG GGTTGGGAAC ACCAACAACC AGCTACAGTT 
CACGGATACC AGATGAAGTC ATATGTAGAC AATTTGGCAA AAGAAAGGCT TGAAGCCTTA 
CAATCCCGTG GAGAGATCCC GACATCGAGA TCTATGGCGC TTGGTACATT GAGCTATACA 
CAGCAACTTG CTTTAGCCTT GAAACAGAAG TCCCAGAATG GTCTAACCCC TGGACCAGCT 
CCTGGGTTTG AGAATTTTGC TGGGTCTAGA AGCATATCGC GACAATCTGA AAGATCTTAT 
TACGGTGTTC CATCTTCTGG CAATACTGAT ACTGTTGGCG CAGCAGTAGC CAATGAGAAA 
AAATATAGTA GCATGCCAGA TATCTCAGGA TTGTCTATGT CCGCAAGGAA CATGCATTTA 
CCAAACAACA AGAGTGGATA CTGGGATCCG TCAAGTGGAG GAGGAGGGTA TGGTGCGTCT 
TATGGTCGGT TAAGCAATGA ATCATCGTTA TATTCTAATT TGGGGTCACG GGTGGGAGTA 
CCCTCGACTT ATGATGACAT TTCTCAATCA AGAGGAGGCT ACAGAGATGC CTACAGTTTG 
CCACAGAGTG CAACAACAGG GACCGGATCG CTTTGGTCCA GACAGCCCTT TGAGCAGTTT 
GGTGTAGCGG AGAGGAATGG TGCTGTTGGT GAGGAGCTCA GGAATAGATC GAATCCGATC 
AATATAGACA ACAACGCTTC TTCTAATGTT GATGCAGAGG CTAAGCTTCT TCAGTCGTTC 
AGGCACTGTA TTCTAAAGCT TATTAAACTT GAAGGATCCG AGTGGTTGTT TGGACAAAGC 
GATGGAGTTG ATGAAGAACT GATTGACCGG GTAGCTGCAC GAGAGAAGTT TATCTATGAA 
GCTGAAGCTC GAGAAATAAA CCAGGTGGGT CACATGGGGG AGCCACTAAT TTCATCGGTT 
CCTAACTGTG GAGATGGTTG CGTTTGGAGA GCTGATTTGA TTGTGAGCTT TGGAGTTTGG 
TGCATTCACC GTGTCCTTGA CTTGTCTCTC ATGGAGAGTC GGCCTGAGCT TTGGGGAAAG 
TACACTTACG TTCTCAACCG CCTACAGGGA GTGATTGATC CGGCGTTCTC AAAGCTGCGG 
ACACCAATGA CACCGTGCTT TTGCCTTCAG ATTCCAGCGA GCCACCAGAG AGCGAGTCCG 
ACTTCAGCTA ACGGAATGTT ACCTCCGGCT GCAAAACCGG CTAAAGGCAA ATGCACAACC 
GCAGTCACAC TTCTTGATCT AATCAAAGAC GTTGAAATGG CAATCTCTTG TAGAAAAGGC 
CGAACCGGTA CAGCTGCAGG TGATGTGGCT TTCCCAAAGG GGAAAGAGAA TTTGGCTTCG 
GTTTTGAAGC GGTATAAACG TCGGTTATCG AATAAACCAG TAGGTATGAA TCAGGATGGA 
CCCGGTTCAA GAAAAAACGT GACTGCGTAC GGATCATTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195948.1
CDS	629..4513
Translation	MEAEIVNVRPQLGFIQRMVPALLPVLLVSVGYIDPGKWVANIEGGARFGYDLVAITLLFNFAAILCQYVAARISVVTGKHLAQICNEEYDKWTCMFLGIQAEFSAILLDLTMVVGVAHALNLLFGVELSTGVFLAAMDAFLFPVFASFLENGMANTVSIYSAGLVLLLYVSGVLLSQSEIPLSMNGVLTRLNGESAFALMGLLGASIVPHNFYIHSYFAGESTSSSDVDKSSLCQDHLFAIFGVFSGLSLVNYVLMNAAANVFHSTGLVVLTFHDALSLMEQVFMSPLIPVVFLMLLFFSSQITALAWAFGGEVVLHDFLKIEIPAWLHRATIRILAVAPALYCVWTSGADGIYQLLIFTQVLVAMMLPCSVIPLFRIASSRQIMGVHKIPQVGEFLALTTFLGFLGLNVVFVVEMVFGSSDWAGGLRWNTVMGTSIQYTTLLVSSCASLCLILWLAATPLKSASNRAEAQIWNMDAQNALSYPSVQEEEIERTETRRNEDESIVRLESRVKDQLDTTSVTSSVYDLPENILMTDQEIRSSPPEERELDVKYSTSQVSSLKEDSDVKEQSVLQSTVVNEVSDKDLIVETKMAKIEPMSPVEKIVSMENNSKFIEKDVEGVSWETEEATKAAPTSNFTVGSDGPPSFRSLSGEGGSGTGSLSRLQGLGRAARRHLSAILDEFWGHLYDFHGQLVAEARAKKLDQLFGTDQKSASSMKADSFGKDISSGYCMSPTAKGMDSQMTSSLYDSLKQQRTPGSIDSLYGLQRGSSPSPLVNRMQMLGAYGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGYQMKSYVDNLAKERLEALQSRGEIPTSRSMALGTLSYTQQLALALKQKSQNGLTPGPAPGFENFAGSRSISRQSERSYYGVPSSGNTDTVGAAVANEKKYSSMPDISGLSMSARNMHLPNNKSGYWDPSSGGGGYGASYGRLSNESSLYSNLGSRVGVPSTYDDISQSRGGYRDAYSLPQSATTGTGSLWSRQPFEQFGVAERNGAVGEELRNRSNPINIDNNASSNVDAEAKLLQSFRHCILKLIKLEGSEWLFGQSDGVDEELIDRVAAREKFIYEAEAREINQVGHMGEPLISSVPNCGDGCVWRADLIVSFGVWCIHRVLDLSLMESRPELWGKYTYVLNRLQGVIDPAFSKLRTPMTPCFCLQIPASHQRASPTSANGMLPPAAKPAKGKCTTAVTLLDLIKDVEMAISCRKGRTGTAAGDVAFPKGKENLASVLKRYKRRLSNKPVGMNQDGPGSRKNVTAYGSLG

Target ORF information:

RefSeq Version	NM_120406.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NRAMP metal ion transporter family protein (EIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_120406.5

ORF Insert Sequence:

ATGGAAGCTG AAATTGTGAA TGTGAGACCT CAGCTAGGGT TTATCCAGAG AATGGTTCCT 
GCTCTACTTC CTGTCCTTTT GGTTTCTGTC GGATATATTG ATCCCGGGAA ATGGGTTGCA 
AATATCGAAG GAGGTGCTCG TTTCGGGTAT GACTTGGTGG CAATTACTCT GCTTTTCAAT 
TTTGCCGCCA TCTTATGCCA ATATGTTGCA GCTCGCATAA GCGTTGTGAC TGGTAAACAC 
TTGGCTCAGA TCTGCAATGA AGAATATGAC AAGTGGACGT GCATGTTCTT GGGCATTCAG 
GCGGAGTTCT CAGCAATTCT GCTCGACCTT ACCATGGTTG TGGGAGTTGC GCATGCACTT 
AACCTTTTGT TTGGGGTGGA GTTATCCACT GGAGTGTTTT TGGCCGCCAT GGATGCGTTT 
TTATTTCCTG TTTTCGCCTC TTTCCTTGAA AATGGTATGG CAAATACAGT ATCCATTTAC 
TCTGCAGGCC TGGTATTACT TCTCTATGTA TCTGGCGTCT TGCTGAGTCA GTCTGAGATC 
CCACTCTCTA TGAATGGAGT GTTAACTCGG TTAAATGGAG AGAGCGCATT CGCACTGATG 
GGTCTTCTTG GCGCAAGCAT CGTCCCTCAC AATTTTTATA TCCATTCTTA TTTTGCTGGG 
GAAAGTACAT CTTCGTCTGA TGTCGACAAG AGCAGCTTGT GTCAAGACCA TTTGTTCGCC 
ATCTTTGGTG TCTTCAGCGG ACTGTCACTT GTAAATTATG TATTGATGAA TGCAGCAGCT 
AATGTGTTTC ACAGTACTGG CCTTGTGGTA CTGACTTTTC ACGATGCCTT GTCACTAATG 
GAGCAGGTAT TTATGAGTCC GCTCATTCCA GTGGTCTTTT TGATGCTCTT GTTCTTCTCT 
AGTCAAATTA CCGCACTAGC TTGGGCTTTC GGTGGAGAGG TCGTCCTGCA TGACTTCCTG 
AAGATAGAAA TACCCGCTTG GCTTCATCGT GCTACAATCA GAATTCTTGC AGTTGCTCCT 
GCGCTTTATT GTGTATGGAC ATCTGGTGCA GACGGAATAT ACCAGTTACT TATATTCACC 
CAGGTCTTGG TGGCAATGAT GCTTCCTTGC TCGGTAATAC CGCTTTTCCG CATTGCTTCG 
TCGAGACAAA TCATGGGTGT CCATAAAATC CCTCAGGTTG GCGAGTTCCT CGCACTTACA 
ACGTTTTTGG GATTTCTGGG GTTGAATGTT GTTTTTGTTG TTGAGATGGT ATTTGGGAGC 
AGTGACTGGG CTGGTGGTTT GAGATGGAAT ACCGTGATGG GCACCTCGAT TCAGTACACC 
ACTCTGCTTG TATCGTCATG TGCATCCTTA TGCCTGATAC TCTGGCTGGC AGCCACGCCG 
CTGAAATCTG CGAGTAACAG AGCGGAAGCT CAAATATGGA ACATGGATGC TCAAAATGCT 
TTATCTTATC CATCTGTTCA AGAAGAGGAA ATTGAAAGAA CAGAAACAAG GAGGAACGAA 
GACGAATCAA TAGTGCGGTT GGAAAGCAGG GTAAAGGATC AGTTGGATAC TACGTCTGTT 
ACTAGCTCGG TCTATGATTT GCCAGAGAAC ATTCTAATGA CGGATCAAGA AATCCGTTCG 
AGCCCTCCAG AGGAAAGAGA GTTGGATGTA AAGTACTCTA CCTCTCAAGT TAGTAGTCTT 
AAGGAAGACT CTGATGTAAA GGAACAGTCT GTATTGCAGT CAACAGTGGT TAATGAGGTC 
AGTGATAAGG ATCTGATTGT TGAAACAAAG ATGGCGAAAA TTGAACCAAT GAGTCCTGTG 
GAGAAGATTG TTAGCATGGA GAATAACAGC AAGTTTATTG AAAAGGATGT TGAAGGGGTT 
TCATGGGAAA CAGAAGAAGC TACCAAAGCT GCTCCTACAA GCAACTTTAC TGTCGGATCT 
GATGGTCCTC CTTCATTCCG CAGCTTAAGT GGGGAAGGGG GAAGTGGGAC TGGAAGCCTT 
TCACGGTTGC AAGGTTTGGG ACGTGCTGCC CGGAGACACT TATCTGCGAT CCTTGATGAA 
TTTTGGGGAC ATTTATATGA TTTTCATGGG CAATTGGTTG CTGAAGCCAG GGCAAAGAAA 
CTAGATCAGC TGTTTGGCAC TGATCAAAAG TCAGCCTCTT CTATGAAAGC AGATTCGTTT 
GGAAAAGACA TTAGCAGTGG ATATTGCATG TCACCAACTG CGAAGGGAAT GGATTCACAG 
ATGACTTCAA GTTTATATGA TTCACTGAAG CAGCAGAGGA CACCGGGAAG TATCGATTCG 
TTGTATGGAT TACAAAGAGG TTCGTCACCG TCACCGTTGG TCAACCGTAT GCAGATGTTG 
GGTGCATATG GTAACACCAC TAATAATAAT AATGCTTACG AATTGAGTGA GAGAAGATAC 
TCTAGCCTGC GTGCTCCATC ATCTTCAGAG GGTTGGGAAC ACCAACAACC AGCTACAGTT 
CACGGATACC AGATGAAGTC ATATGTAGAC AATTTGGCAA AAGAAAGGCT TGAAGCCTTA 
CAATCCCGTG GAGAGATCCC GACATCGAGA TCTATGGCGC TTGGTACATT GAGCTATACA 
CAGCAACTTG CTTTAGCCTT GAAACAGAAG TCCCAGAATG GTCTAACCCC TGGACCAGCT 
CCTGGGTTTG AGAATTTTGC TGGGTCTAGA AGCATATCGC GACAATCTGA AAGATCTTAT 
TACGGTGTTC CATCTTCTGG CAATACTGAT ACTGTTGGCG CAGCAGTAGC CAATGAGAAA 
AAATATAGTA GCATGCCAGA TATCTCAGGA TTGTCTATGT CCGCAAGGAA CATGCATTTA 
CCAAACAACA AGAGTGGATA CTGGGATCCG TCAAGTGGAG GAGGAGGGTA TGGTGCGTCT 
TATGGTCGGT TAAGCAATGA ATCATCGTTA TATTCTAATT TGGGGTCACG GGTGGGAGTA 
CCCTCGACTT ATGATGACAT TTCTCAATCA AGAGGAGGCT ACAGAGATGC CTACAGTTTG 
CCACAGAGTG CAACAACAGG GACCGGATCG CTTTGGTCCA GACAGCCCTT TGAGCAGTTT 
GGTGTAGCGG AGAGGAATGG TGCTGTTGGT GAGGAGCTCA GGAATAGATC GAATCCGATC 
AATATAGACA ACAACGCTTC TTCTAATGTT GATGCAGAGG CTAAGCTTCT TCAGTCGTTC 
AGGCACTGTA TTCTAAAGCT TATTAAACTT GAAGGATCCG AGTGGTTGTT TGGACAAAGC 
GATGGAGTTG ATGAAGAACT GATTGACCGG GTAGCTGCAC GAGAGAAGTT TATCTATGAA 
GCTGAAGCTC GAGAAATAAA CCAGGTGGGT CACATGGGGG AGCCACTAAT TTCATCGGTT 
CCTAACTGTG GAGATGGTTG CGTTTGGAGA GCTGATTTGA TTGTGAGCTT TGGAGTTTGG 
TGCATTCACC GTGTCCTTGA CTTGTCTCTC ATGGAGAGTC GGCCTGAGCT TTGGGGAAAG 
TACACTTACG TTCTCAACCG CCTACAGGGA GTGATTGATC CGGCGTTCTC AAAGCTGCGG 
ACACCAATGA CACCGTGCTT TTGCCTTCAG ATTCCAGCGA GCCACCAGAG AGCGAGTCCG 
ACTTCAGCTA ACGGAATGTT ACCTCCGGCT GCAAAACCGG CTAAAGGCAA ATGCACAACC 
GCAGTCACAC TTCTTGATCT AATCAAAGAC GTTGAAATGG CAATCTCTTG TAGAAAAGGC 
CGAACCGGTA CAGCTGCAGG TGATGTGGCT TTCCCAAAGG GGAAAGAGAA TTTGGCTTCG 
GTTTTGAAGC GGTATAAACG TCGGTTATCG AATAAACCAG TAGGTATGAA TCAGGATGGA 
CCCGGTTCAA GAAAAAACGT GACTGCGTAC GGATCATTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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EIN2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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