BGAL3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	BGAL3
Entrez Gene ID	829788
Full Name	beta-galactosidase 3
Synonyms	F23E13.200,F23E13_200,beta-galactosidase 3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_119799.4	NP_568001.1	beta-galactosidase 3
NM_179175.2	NP_849506.1	beta-galactosidase 3

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	BGAL3	NP_568001.1
Oryza sativa (rice)	Os03g0165400	NP_001049068.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate the transcript accumulation of the beta-galactosidases (BGAL) genes AtBGAL1 (At3g13750), AtBGAL2 (At3g52840), AtBGAL3 (At4g36360), AtBGAL4 (At5g56870), AtBGAL5 (At1g45130) and AtBGAL12 (At4g26140) along the plant development, as well as their subcellular location by the construction of transgenic plants producing the enhanced green fluorescent protein (eGFP) fused to the six BGAL proteins.
Title: Subcellular location of Arabidopsis thaliana subfamily a1 ?-galactosidases and developmental regulation of transcript levels?of?their coding genes.

The following BGAL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BGAL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb32047	NM_179175.2 Latest version!	Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb32048	NM_119799.4 Latest version!	Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BGAL3 ( NM_179175.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849506.1 Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.

Price & Availability↑

CloneID	OAb32047
Clone ID Related Accession (Same CDS sequence)	NM_179175.2
Accession Version	NM_179175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	beta-galactosidase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Apr 18, 2007 this sequence version replaced NM_179175.1.

ATGCGGGAGA TGGGGACTGG AGATTCTGCT TCGAGACTGA TTCTCTGGTT CTGCTTAGGG 
TTTTTGATTC TAGGTGTTGG ATTTGTGCAA TGTGGAGTTA CTTATGACCG GAAAGCACTT 
CTCATTAATG GACAGAGACG AATCCTCTTC TCTGGCTCCA TACATTACCC TCGAAGCACT 
CCTGATATGT GGGAAGATTT GATTCAGAAG GCGAAAGATG GAGGCATTGA TGTGATTGAG 
ACGTATGTGT TCTGGAATCT TCATGAGCCT TCTCCTGGAA AATACGATTT TGAAGGGAGA 
AATGATTTGG TGAGATTCGT GAAGACGATT CACAAAGCTG GTTTATATGC TCATCTTCGA 
ATTGGACCTT ATGTTTGCGC CGAGTGGAAT TTTGGAGGAT TCCCTGTTTG GCTCAAGTAT 
GTTCCTGGAA TCAGCTTCAG AACAGATAAT GAGCCTTTTA AGAGAGCTAT GAAAGGATTC 
ACAGAGAGAA TAGTTGAGCT GATGAAGAGT GAGAACTTGT TCGAGTCCCA GGGTGGTCCC 
ATTATCCTTT CTCAGATTGA GAATGAGTAT GGAAGACAAG GGCAGTTATT GGGCGCTGAA 
GGTCACAACT ACATGACATG GGCAGCTAAA ATGGCTATAG CAACTGAGAC TGGTGTCCCG 
TGGGTCATGT GCAAAGAAGA CGATGCCCCT GACCCTGTGA TAAACACATG TAACGGTTTC 
TACTGTGATT CATTTGCTCC GAACAAGCCA TACAAGCCTT TGATATGGAC AGAGGCATGG 
AGCGGCTGGT TCACTGAGTT CGGTGGGCCA ATGCACCATA GACCAGTTCA GGATCTGGCG 
TTTGGTGTTG CACGTTTCAT ACAAAAAGGA GGATCTTTTG TTAACTACTA CATGTATCAT 
GGAGGAACTA ACTTTGGAAG AACAGCCGGA GGTCCATTTG TCACCACCAG CTATGATTAT 
GATGCTCCGA TCGACGAATA TGGTTTAATC AGACAACCTA AATATGGTCA CCTAAAGGAA 
CTCCACAGAG CTATCAAGAT GTGTGAGAAG GCTCTGGTGT CAGCTGATCC CGTTGTTACA 
TCAATAGGAA ACAAGCAACA AGCTCATGTA TACTCTGCAG AATCAGGAGA TTGTTCGGCT 
TTCCTAGCTA ACTATGATAC AGAATCGGCA GCAAGAGTTT TGTTTAACAA CGTTCATTAT 
AACTTACCTC CTTGGTCAAT CAGTATTCTT CCAGACTGTA GAAATGCAGT CTTCAATACT 
GCAAAGGTTG GAGTTCAAAC ATCCCAGATG GAAATGTTGC CTACAGACAC AAAGAATTTC 
CAGTGGGAGA GTTATTTGGA AGATCTTTCT TCTCTAGATG ACAGCTCCAC ATTCACCACT 
CACGGACTCT TGGAGCAGAT AAATGTCACG CGTGACACAA GCGATTATCT TTGGTACATG 
ACGAGTGTTG ATATCGGTGA CAGTGAATCA TTCTTGCATG GAGGAGAGCT ACCAACTCTC 
ATTATTCAAT CTACAGGCCA TGCCGTGCAT ATATTTGTCA ATGGACAGCT TTCAGGTTCT 
GCCTTTGGAA CAAGGCAAAA CAGGAGATTT ACTTATCAGG GAAAGATCAA TCTGCATTCT 
GGAACTAACA GAATTGCGTT GCTGAGTGTT GCTGTTGGAT TACCAAATGT GGGTGGACAC 
TTTGAGTCAT GGAATACTGG AATCTTGGGT CCGGTGGCAT TGCATGGGTT GAGTCAAGGG 
AAAATGGATT TGTCATGGCA GAAATGGACA TACCAGGTGG GACTGAAAGG TGAAGCTATG 
AATCTTGCAT TTCCAACCAA CACTCCCTCT ATTGGATGGA TGGATGCGTC CTTAACTGTA 
CAAAAGCCTC AGCCTTTGAC ATGGCACAAG ACTTATTTTG ATGCGCCCGA AGGAAATGAG 
CCGCTTGCTT TGGACATGGA AGGAATGGGA AAAGGTCAGA TTTGGGTGAA CGGTGAGAGC 
ATCGGAAGAT ACTGGACAGC ATTTGCGACT GGTGACTGCA GTCATTGTAG CTACACTGGA 
ACATATAAAC CGAACAAATG CCAAACCGGC TGTGGTCAAC CTACACAAAG GTGGTACCAT 
GTGCCGAGAG CTTGGTTAAA GCCGAGCCAA AATCTGTTGG TCATATTCGA GGAGCTTGGT 
GGAAACCCAT CAACTGTCTC TTTGGTCAAA AGATCAGTAT CAGGAGTTTG TGCTGAAGTT 
TCTGAATACC ACCCGAATAT CAAGAACTGG CAAATCGAAA GCTACGGAAA GGGACAGACA 
TTTCATAGAC CCAAGGTTCA TTTGAAATGT AGTCCAGGTC AAGCAATTGC ATCCATCAAA 
TTTGCAAGCT TTGGAACTCC TTTAGGAACC TGCGGGAGTT ACCAACAAGG AGAGTGTCAC 
GCAGCTACCT CATACGCTAT CTTAGAGAGG TGTGTAGGGA AAGCGAGATG CGCAGTGACG 
ATATCCAACA GCAATTTCGG GAAAGACCCG TGTCCAAATG TGCTGAAACG GTTGACTGTT 
GAAGCAGTTT GTGCCCCAGA GACATCTGTA AGCACTTGGA GACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849506.1
CDS	119..2686
Translation	MREMGTGDSASRLILWFCLGFLILGVGFVQCGVTYDRKALLINGQRRILFSGSIHYPRSTPDMWEDLIQKAKDGGIDVIETYVFWNLHEPSPGKYDFEGRNDLVRFVKTIHKAGLYAHLRIGPYVCAEWNFGGFPVWLKYVPGISFRTDNEPFKRAMKGFTERIVELMKSENLFESQGGPIILSQIENEYGRQGQLLGAEGHNYMTWAAKMAIATETGVPWVMCKEDDAPDPVINTCNGFYCDSFAPNKPYKPLIWTEAWSGWFTEFGGPMHHRPVQDLAFGVARFIQKGGSFVNYYMYHGGTNFGRTAGGPFVTTSYDYDAPIDEYGLIRQPKYGHLKELHRAIKMCEKALVSADPVVTSIGNKQQAHVYSAESGDCSAFLANYDTESAARVLFNNVHYNLPPWSISILPDCRNAVFNTAKVGVQTSQMEMLPTDTKNFQWESYLEDLSSLDDSSTFTTHGLLEQINVTRDTSDYLWYMTSVDIGDSESFLHGGELPTLIIQSTGHAVHIFVNGQLSGSAFGTRQNRRFTYQGKINLHSGTNRIALLSVAVGLPNVGGHFESWNTGILGPVALHGLSQGKMDLSWQKWTYQVGLKGEAMNLAFPTNTPSIGWMDASLTVQKPQPLTWHKTYFDAPEGNEPLALDMEGMGKGQIWVNGESIGRYWTAFATGDCSHCSYTGTYKPNKCQTGCGQPTQRWYHVPRAWLKPSQNLLVIFEELGGNPSTVSLVKRSVSGVCAEVSEYHPNIKNWQIESYGKGQTFHRPKVHLKCSPGQAIASIKFASFGTPLGTCGSYQQGECHAATSYAILERCVGKARCAVTISNSNFGKDPCPNVLKRLTVEAVCAPETSVSTWRP

Target ORF information:

RefSeq Version	NM_179175.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179175.2

ORF Insert Sequence:

ATGCGGGAGA TGGGGACTGG AGATTCTGCT TCGAGACTGA TTCTCTGGTT CTGCTTAGGG 
TTTTTGATTC TAGGTGTTGG ATTTGTGCAA TGTGGAGTTA CTTATGACCG GAAAGCACTT 
CTCATTAATG GACAGAGACG AATCCTCTTC TCTGGCTCCA TACATTACCC TCGAAGCACT 
CCTGATATGT GGGAAGATTT GATTCAGAAG GCGAAAGATG GAGGCATTGA TGTGATTGAG 
ACGTATGTGT TCTGGAATCT TCATGAGCCT TCTCCTGGAA AATACGATTT TGAAGGGAGA 
AATGATTTGG TGAGATTCGT GAAGACGATT CACAAAGCTG GTTTATATGC TCATCTTCGA 
ATTGGACCTT ATGTTTGCGC CGAGTGGAAT TTTGGAGGAT TCCCTGTTTG GCTCAAGTAT 
GTTCCTGGAA TCAGCTTCAG AACAGATAAT GAGCCTTTTA AGAGAGCTAT GAAAGGATTC 
ACAGAGAGAA TAGTTGAGCT GATGAAGAGT GAGAACTTGT TCGAGTCCCA GGGTGGTCCC 
ATTATCCTTT CTCAGATTGA GAATGAGTAT GGAAGACAAG GGCAGTTATT GGGCGCTGAA 
GGTCACAACT ACATGACATG GGCAGCTAAA ATGGCTATAG CAACTGAGAC TGGTGTCCCG 
TGGGTCATGT GCAAAGAAGA CGATGCCCCT GACCCTGTGA TAAACACATG TAACGGTTTC 
TACTGTGATT CATTTGCTCC GAACAAGCCA TACAAGCCTT TGATATGGAC AGAGGCATGG 
AGCGGCTGGT TCACTGAGTT CGGTGGGCCA ATGCACCATA GACCAGTTCA GGATCTGGCG 
TTTGGTGTTG CACGTTTCAT ACAAAAAGGA GGATCTTTTG TTAACTACTA CATGTATCAT 
GGAGGAACTA ACTTTGGAAG AACAGCCGGA GGTCCATTTG TCACCACCAG CTATGATTAT 
GATGCTCCGA TCGACGAATA TGGTTTAATC AGACAACCTA AATATGGTCA CCTAAAGGAA 
CTCCACAGAG CTATCAAGAT GTGTGAGAAG GCTCTGGTGT CAGCTGATCC CGTTGTTACA 
TCAATAGGAA ACAAGCAACA AGCTCATGTA TACTCTGCAG AATCAGGAGA TTGTTCGGCT 
TTCCTAGCTA ACTATGATAC AGAATCGGCA GCAAGAGTTT TGTTTAACAA CGTTCATTAT 
AACTTACCTC CTTGGTCAAT CAGTATTCTT CCAGACTGTA GAAATGCAGT CTTCAATACT 
GCAAAGGTTG GAGTTCAAAC ATCCCAGATG GAAATGTTGC CTACAGACAC AAAGAATTTC 
CAGTGGGAGA GTTATTTGGA AGATCTTTCT TCTCTAGATG ACAGCTCCAC ATTCACCACT 
CACGGACTCT TGGAGCAGAT AAATGTCACG CGTGACACAA GCGATTATCT TTGGTACATG 
ACGAGTGTTG ATATCGGTGA CAGTGAATCA TTCTTGCATG GAGGAGAGCT ACCAACTCTC 
ATTATTCAAT CTACAGGCCA TGCCGTGCAT ATATTTGTCA ATGGACAGCT TTCAGGTTCT 
GCCTTTGGAA CAAGGCAAAA CAGGAGATTT ACTTATCAGG GAAAGATCAA TCTGCATTCT 
GGAACTAACA GAATTGCGTT GCTGAGTGTT GCTGTTGGAT TACCAAATGT GGGTGGACAC 
TTTGAGTCAT GGAATACTGG AATCTTGGGT CCGGTGGCAT TGCATGGGTT GAGTCAAGGG 
AAAATGGATT TGTCATGGCA GAAATGGACA TACCAGGTGG GACTGAAAGG TGAAGCTATG 
AATCTTGCAT TTCCAACCAA CACTCCCTCT ATTGGATGGA TGGATGCGTC CTTAACTGTA 
CAAAAGCCTC AGCCTTTGAC ATGGCACAAG ACTTATTTTG ATGCGCCCGA AGGAAATGAG 
CCGCTTGCTT TGGACATGGA AGGAATGGGA AAAGGTCAGA TTTGGGTGAA CGGTGAGAGC 
ATCGGAAGAT ACTGGACAGC ATTTGCGACT GGTGACTGCA GTCATTGTAG CTACACTGGA 
ACATATAAAC CGAACAAATG CCAAACCGGC TGTGGTCAAC CTACACAAAG GTGGTACCAT 
GTGCCGAGAG CTTGGTTAAA GCCGAGCCAA AATCTGTTGG TCATATTCGA GGAGCTTGGT 
GGAAACCCAT CAACTGTCTC TTTGGTCAAA AGATCAGTAT CAGGAGTTTG TGCTGAAGTT 
TCTGAATACC ACCCGAATAT CAAGAACTGG CAAATCGAAA GCTACGGAAA GGGACAGACA 
TTTCATAGAC CCAAGGTTCA TTTGAAATGT AGTCCAGGTC AAGCAATTGC ATCCATCAAA 
TTTGCAAGCT TTGGAACTCC TTTAGGAACC TGCGGGAGTT ACCAACAAGG AGAGTGTCAC 
GCAGCTACCT CATACGCTAT CTTAGAGAGG TGTGTAGGGA AAGCGAGATG CGCAGTGACG 
ATATCCAACA GCAATTTCGG GAAAGACCCG TGTCCAAATG TGCTGAAACG GTTGACTGTT 
GAAGCAGTTT GTGCCCCAGA GACATCTGTA AGCACTTGGA GACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BGAL3 ( NM_119799.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568001.1 Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.

Price & Availability↑

CloneID	OAb32048
Clone ID Related Accession (Same CDS sequence)	NM_119799.4
Accession Version	NM_119799.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	beta-galactosidase 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119799.3.

ATGCGGGAGA TGGGGACTGG AGATTCTGCT TCGAGACTGA TTCTCTGGTT CTGCTTAGGG 
TTTTTGATTC TAGGTGTTGG ATTTGTGCAA TGTGGAGTTA CTTATGACCG GAAAGCACTT 
CTCATTAATG GACAGAGACG AATCCTCTTC TCTGGCTCCA TACATTACCC TCGAAGCACT 
CCTGATATGT GGGAAGATTT GATTCAGAAG GCGAAAGATG GAGGCATTGA TGTGATTGAG 
ACGTATGTGT TCTGGAATCT TCATGAGCCT TCTCCTGGAA AATACGATTT TGAAGGGAGA 
AATGATTTGG TGAGATTCGT GAAGACGATT CACAAAGCTG GTTTATATGC TCATCTTCGA 
ATTGGACCTT ATGTTTGCGC CGAGTGGAAT TTTGGAGGAT TCCCTGTTTG GCTCAAGTAT 
GTTCCTGGAA TCAGCTTCAG AACAGATAAT GAGCCTTTTA AGAGAGCTAT GAAAGGATTC 
ACAGAGAGAA TAGTTGAGCT GATGAAGAGT GAGAACTTGT TCGAGTCCCA GGGTGGTCCC 
ATTATCCTTT CTCAGATTGA GAATGAGTAT GGAAGACAAG GGCAGTTATT GGGCGCTGAA 
GGTCACAACT ACATGACATG GGCAGCTAAA ATGGCTATAG CAACTGAGAC TGGTGTCCCG 
TGGGTCATGT GCAAAGAAGA CGATGCCCCT GACCCTGTGA TAAACACATG TAACGGTTTC 
TACTGTGATT CATTTGCTCC GAACAAGCCA TACAAGCCTT TGATATGGAC AGAGGCATGG 
AGCGGCTGGT TCACTGAGTT CGGTGGGCCA ATGCACCATA GACCAGTTCA GGATCTGGCG 
TTTGGTGTTG CACGTTTCAT ACAAAAAGGA GGATCTTTTG TTAACTACTA CATGTATCAT 
GGAGGAACTA ACTTTGGAAG AACAGCCGGA GGTCCATTTG TCACCACCAG CTATGATTAT 
GATGCTCCGA TCGACGAATA TGGTTTAATC AGACAACCTA AATATGGTCA CCTAAAGGAA 
CTCCACAGAG CTATCAAGAT GTGTGAGAAG GCTCTGGTGT CAGCTGATCC CGTTGTTACA 
TCAATAGGAA ACAAGCAACA AGCTCATGTA TACTCTGCAG AATCAGGAGA TTGTTCGGCT 
TTCCTAGCTA ACTATGATAC AGAATCGGCA GCAAGAGTTT TGTTTAACAA CGTTCATTAT 
AACTTACCTC CTTGGTCAAT CAGTATTCTT CCAGACTGTA GAAATGCAGT CTTCAATACT 
GCAAAGGTTG GAGTTCAAAC ATCCCAGATG GAAATGTTGC CTACAGACAC AAAGAATTTC 
CAGTGGGAGA GTTATTTGGA AGATCTTTCT TCTCTAGATG ACAGCTCCAC ATTCACCACT 
CACGGACTCT TGGAGCAGAT AAATGTCACG CGTGACACAA GCGATTATCT TTGGTACATG 
ACGAGTGTTG ATATCGGTGA CAGTGAATCA TTCTTGCATG GAGGAGAGCT ACCAACTCTC 
ATTATTCAAT CTACAGGCCA TGCCGTGCAT ATATTTGTCA ATGGACAGCT TTCAGGTTCT 
GCCTTTGGAA CAAGGCAAAA CAGGAGATTT ACTTATCAGG GAAAGATCAA TCTGCATTCT 
GGAACTAACA GAATTGCGTT GCTGAGTGTT GCTGTTGGAT TACCAAATGT GGGTGGACAC 
TTTGAGTCAT GGAATACTGG AATCTTGGGT CCGGTGGCAT TGCATGGGTT GAGTCAAGGG 
AAAATGGATT TGTCATGGCA GAAATGGACA TACCAGGTGG GACTGAAAGG TGAAGCTATG 
AATCTTGCAT TTCCAACCAA CACTCCCTCT ATTGGATGGA TGGATGCGTC CTTAACTGTA 
CAAAAGCCTC AGCCTTTGAC ATGGCACAAG ACTTATTTTG ATGCGCCCGA AGGAAATGAG 
CCGCTTGCTT TGGACATGGA AGGAATGGGA AAAGGTCAGA TTTGGGTGAA CGGTGAGAGC 
ATCGGAAGAT ACTGGACAGC ATTTGCGACT GGTGACTGCA GTCATTGTAG CTACACTGGA 
ACATATAAAC CGAACAAATG CCAAACCGGC TGTGGTCAAC CTACACAAAG GTGGTACCAT 
GTGCCGAGAG CTTGGTTAAA GCCGAGCCAA AATCTGTTGG TCATATTCGA GGAGCTTGGT 
GGAAACCCAT CAACTGTCTC TTTGGTCAAA AGATCAGTAT CAGGAGTTTG TGCTGAAGTT 
TCTGAATACC ACCCGAATAT CAAGAACTGG CAAATCGAAA GCTACGGAAA GGGACAGACA 
TTTCATAGAC CCAAGGTTCA TTTGAAATGT AGTCCAGGTC AAGCAATTGC ATCCATCAAA 
TTTGCAAGCT TTGGAACTCC TTTAGGAACC TGCGGGAGTT ACCAACAAGG AGAGTGTCAC 
GCAGCTACCT CATACGCTAT CTTAGAGAGG AAGTGTGTAG GGAAAGCGAG ATGCGCAGTG 
ACGATATCCA ACAGCAATTT CGGGAAAGAC CCGTGTCCAA ATGTGCTGAA ACGGTTGACT 
GTTGAAGCAG TTTGTGCCCC AGAGACATCT GTAAGCACTT GGAGACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568001.1
CDS	324..2894
Translation	MREMGTGDSASRLILWFCLGFLILGVGFVQCGVTYDRKALLINGQRRILFSGSIHYPRSTPDMWEDLIQKAKDGGIDVIETYVFWNLHEPSPGKYDFEGRNDLVRFVKTIHKAGLYAHLRIGPYVCAEWNFGGFPVWLKYVPGISFRTDNEPFKRAMKGFTERIVELMKSENLFESQGGPIILSQIENEYGRQGQLLGAEGHNYMTWAAKMAIATETGVPWVMCKEDDAPDPVINTCNGFYCDSFAPNKPYKPLIWTEAWSGWFTEFGGPMHHRPVQDLAFGVARFIQKGGSFVNYYMYHGGTNFGRTAGGPFVTTSYDYDAPIDEYGLIRQPKYGHLKELHRAIKMCEKALVSADPVVTSIGNKQQAHVYSAESGDCSAFLANYDTESAARVLFNNVHYNLPPWSISILPDCRNAVFNTAKVGVQTSQMEMLPTDTKNFQWESYLEDLSSLDDSSTFTTHGLLEQINVTRDTSDYLWYMTSVDIGDSESFLHGGELPTLIIQSTGHAVHIFVNGQLSGSAFGTRQNRRFTYQGKINLHSGTNRIALLSVAVGLPNVGGHFESWNTGILGPVALHGLSQGKMDLSWQKWTYQVGLKGEAMNLAFPTNTPSIGWMDASLTVQKPQPLTWHKTYFDAPEGNEPLALDMEGMGKGQIWVNGESIGRYWTAFATGDCSHCSYTGTYKPNKCQTGCGQPTQRWYHVPRAWLKPSQNLLVIFEELGGNPSTVSLVKRSVSGVCAEVSEYHPNIKNWQIESYGKGQTFHRPKVHLKCSPGQAIASIKFASFGTPLGTCGSYQQGECHAATSYAILERKCVGKARCAVTISNSNFGKDPCPNVLKRLTVEAVCAPETSVSTWRP

Target ORF information:

RefSeq Version	NM_119799.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana beta-galactosidase 3 (BGAL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119799.4

ORF Insert Sequence:

ATGCGGGAGA TGGGGACTGG AGATTCTGCT TCGAGACTGA TTCTCTGGTT CTGCTTAGGG 
TTTTTGATTC TAGGTGTTGG ATTTGTGCAA TGTGGAGTTA CTTATGACCG GAAAGCACTT 
CTCATTAATG GACAGAGACG AATCCTCTTC TCTGGCTCCA TACATTACCC TCGAAGCACT 
CCTGATATGT GGGAAGATTT GATTCAGAAG GCGAAAGATG GAGGCATTGA TGTGATTGAG 
ACGTATGTGT TCTGGAATCT TCATGAGCCT TCTCCTGGAA AATACGATTT TGAAGGGAGA 
AATGATTTGG TGAGATTCGT GAAGACGATT CACAAAGCTG GTTTATATGC TCATCTTCGA 
ATTGGACCTT ATGTTTGCGC CGAGTGGAAT TTTGGAGGAT TCCCTGTTTG GCTCAAGTAT 
GTTCCTGGAA TCAGCTTCAG AACAGATAAT GAGCCTTTTA AGAGAGCTAT GAAAGGATTC 
ACAGAGAGAA TAGTTGAGCT GATGAAGAGT GAGAACTTGT TCGAGTCCCA GGGTGGTCCC 
ATTATCCTTT CTCAGATTGA GAATGAGTAT GGAAGACAAG GGCAGTTATT GGGCGCTGAA 
GGTCACAACT ACATGACATG GGCAGCTAAA ATGGCTATAG CAACTGAGAC TGGTGTCCCG 
TGGGTCATGT GCAAAGAAGA CGATGCCCCT GACCCTGTGA TAAACACATG TAACGGTTTC 
TACTGTGATT CATTTGCTCC GAACAAGCCA TACAAGCCTT TGATATGGAC AGAGGCATGG 
AGCGGCTGGT TCACTGAGTT CGGTGGGCCA ATGCACCATA GACCAGTTCA GGATCTGGCG 
TTTGGTGTTG CACGTTTCAT ACAAAAAGGA GGATCTTTTG TTAACTACTA CATGTATCAT 
GGAGGAACTA ACTTTGGAAG AACAGCCGGA GGTCCATTTG TCACCACCAG CTATGATTAT 
GATGCTCCGA TCGACGAATA TGGTTTAATC AGACAACCTA AATATGGTCA CCTAAAGGAA 
CTCCACAGAG CTATCAAGAT GTGTGAGAAG GCTCTGGTGT CAGCTGATCC CGTTGTTACA 
TCAATAGGAA ACAAGCAACA AGCTCATGTA TACTCTGCAG AATCAGGAGA TTGTTCGGCT 
TTCCTAGCTA ACTATGATAC AGAATCGGCA GCAAGAGTTT TGTTTAACAA CGTTCATTAT 
AACTTACCTC CTTGGTCAAT CAGTATTCTT CCAGACTGTA GAAATGCAGT CTTCAATACT 
GCAAAGGTTG GAGTTCAAAC ATCCCAGATG GAAATGTTGC CTACAGACAC AAAGAATTTC 
CAGTGGGAGA GTTATTTGGA AGATCTTTCT TCTCTAGATG ACAGCTCCAC ATTCACCACT 
CACGGACTCT TGGAGCAGAT AAATGTCACG CGTGACACAA GCGATTATCT TTGGTACATG 
ACGAGTGTTG ATATCGGTGA CAGTGAATCA TTCTTGCATG GAGGAGAGCT ACCAACTCTC 
ATTATTCAAT CTACAGGCCA TGCCGTGCAT ATATTTGTCA ATGGACAGCT TTCAGGTTCT 
GCCTTTGGAA CAAGGCAAAA CAGGAGATTT ACTTATCAGG GAAAGATCAA TCTGCATTCT 
GGAACTAACA GAATTGCGTT GCTGAGTGTT GCTGTTGGAT TACCAAATGT GGGTGGACAC 
TTTGAGTCAT GGAATACTGG AATCTTGGGT CCGGTGGCAT TGCATGGGTT GAGTCAAGGG 
AAAATGGATT TGTCATGGCA GAAATGGACA TACCAGGTGG GACTGAAAGG TGAAGCTATG 
AATCTTGCAT TTCCAACCAA CACTCCCTCT ATTGGATGGA TGGATGCGTC CTTAACTGTA 
CAAAAGCCTC AGCCTTTGAC ATGGCACAAG ACTTATTTTG ATGCGCCCGA AGGAAATGAG 
CCGCTTGCTT TGGACATGGA AGGAATGGGA AAAGGTCAGA TTTGGGTGAA CGGTGAGAGC 
ATCGGAAGAT ACTGGACAGC ATTTGCGACT GGTGACTGCA GTCATTGTAG CTACACTGGA 
ACATATAAAC CGAACAAATG CCAAACCGGC TGTGGTCAAC CTACACAAAG GTGGTACCAT 
GTGCCGAGAG CTTGGTTAAA GCCGAGCCAA AATCTGTTGG TCATATTCGA GGAGCTTGGT 
GGAAACCCAT CAACTGTCTC TTTGGTCAAA AGATCAGTAT CAGGAGTTTG TGCTGAAGTT 
TCTGAATACC ACCCGAATAT CAAGAACTGG CAAATCGAAA GCTACGGAAA GGGACAGACA 
TTTCATAGAC CCAAGGTTCA TTTGAAATGT AGTCCAGGTC AAGCAATTGC ATCCATCAAA 
TTTGCAAGCT TTGGAACTCC TTTAGGAACC TGCGGGAGTT ACCAACAAGG AGAGTGTCAC 
GCAGCTACCT CATACGCTAT CTTAGAGAGG AAGTGTGTAG GGAAAGCGAG ATGCGCAGTG 
ACGATATCCA ACAGCAATTT CGGGAAAGAC CCGTGTCCAA ATGTGCTGAA ACGGTTGACT 
GTTGAAGCAG TTTGTGCCCC AGAGACATCT GTAAGCACTT GGAGACCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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BGAL3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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