NSF cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSF
Entrez Gene ID	825830
Full Name	AAA-type ATPase family protein
Synonyms	N-ethylmaleimide sensitive factor,T1J1.4,T1J1_4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_116729.4	NP_192400.2	AAA-type ATPase family protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	NSF	NP_192400.2
Oryza sativa (rice)	Os05g0519400	NP_001056063.1
Danio rerio (zebrafish)	nsfb	NP_001019625.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NSF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14321	NM_116729.4 Latest version!	Arabidopsis thaliana AAA-type ATPase family protein (NSF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSF ( NM_116729.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192400.2 Arabidopsis thaliana AAA-type ATPase family protein (NSF), mRNA.

Price & Availability↑

CloneID	OAb14321
Clone ID Related Accession (Same CDS sequence)	NM_116729.4
Accession Version	NM_116729.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	AAA-type ATPase family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_116729.3.

ATGGCGGGTC GTTACGGATC GCAAGTCATG ACGATGACTG TGACCAATAC TCCGTCCGCA 
GATCTAGCTT TTACTAACCT CGCTTACTGC TCTTCTTCCG ATCTCCGTCA ATTCTCCGTT 
CCCGGTTCCG ATCTCTTCCT CGCCAACGTT GCCGATAGCT TCATTCTTTC TCTCTGTGGA 
CATGGAAGCA TCCGTGATGG AAATATTGCT CTCAATGCTA TTCAAAGGCG ACACGCTAGA 
GTTTCGACTG GTGATATGGT ATCTGTTAGC AGATTTGTTC CTCCTGAAAA TTTTGATCTG 
GCTATGCTGA CGCTGGAGTT GGAATTTGTG AAGAAAGGAA CTAAAAGCGA GCAGGTTGAT 
GCTGCTCTCC TTTCGACCCA GCTTAAGAGG AAATACACCA ACCAGGTCTT GACTGTAGGC 
CAGAAAGCGA CATTTGAGTA TCATGGAACT AATTACATTC TTACGGTCAA TCGAGCGGAT 
GTTGAGGGTC AGGATCACAC GAACGGCATT GAAAGAGGGT TGCTCTCTAA AGACACGTAT 
ATTGTGTTTG AAGCATCAAA TGCAAGTGGC ATAAAGATTG TCAACCAACG GGAAGCTGCT 
AGCAGCAATA TATTCAAACA TAAGGAATTT AACCTTGAGT CGCTGGGAAT AGGTGGTCTT 
GGTGCAGAGT TTGCTGATAT ATTTAGAAGA GCTTTTGCTT CTCGTGTATT TCCTCCTCAT 
GTCACCAGCA GACTGGGAAT AAAGCATGTT AAAGGGATGC TTCTTTTTGG ACCTCCTGGT 
ACTGGCAAAA CCCTTATGGC ACGGCAAATT GGAAAGATGC TTAATGGAAA GGATCCAAAG 
ATCGTTAATG GGCCTGAGGT GCTGAGCAAG TTTGTTGGTG AGACAGAAAA GAATGTAAGA 
GACTTATTTG CTGATGCTGA GCAAGACCAG AGGACCCTTG GTGATGCTAG TGAGTTGCAT 
GTCATTATTT TTGATGAGAT TGATGCTATT TGCAAGTCAA GAGGTTCAAC CAGAGATGGC 
ACAGGTGTCC ATGATAGTAT TGTAAACCAG CTTTTGACAA AGATAGATGG TGTGGAGGCC 
TTAAACAATG TTTTGCTGAT TGGAATGACA AACAGAAAGG ATTTGCTTGA TGAAGCTCTT 
TTAAGGCCTG GAAGATTGGA GGTTCAGGTT GAGATAAGCC TTCCTGATGA AGCTGGGCGT 
CTACAAATTC TTCAGATTCA TACAAATAAG ATGAAGGAAA ATTCTTTCCT TGGGACTGAT 
ATCAATCTCC AAGAGCTTGC TGCTCGAACA AAAAATTACA GTGGTGCAGA GCTTGAAGGT 
GTTGTTAAAA GTGCTACATC ATATGCATTA AACAGACAAC TAAGCATGGA TGATCTTACA 
AAGCCTGTGG AAGAAGAGAA TATCAAGATC ACTATGGAGG ATTTTCTCCA CGCTATCTAT 
GAAGTTCAAC CTGCATTTGG AGCCTCCACA GATGACCTAG AGCGCTGCAG ACTCAATGGG 
ATGGTAGATT GTGGTCATCG GCATAATCAC ATTTACAAAA GAGCTATGCT TCTAGTCGAG 
CAAGTTAAAG TTAGTACTAG AAGTCCTCTA GTCACTTGCC TTCTGGAGGG GCCCAGTGGA 
AGTGGGAAAA CTGCTTTAGC AGCAACAATT GGTATCGATA GTGACTTCCC ATATGTTAAG 
ATAGTTTCTG CGGAAACTAT GATTGGTCTT TCTGAGAGCA CAAAATGTGC TCACATTGTC 
AAGGTCTTTG AGGATGCATA TAAATCTCCA ATGAGCATCA TTATCCTTGA CGATATTGAA 
AGATTGTTGG AATTCATAGC AATCGGTCCT CGCTTTTCAA ACATAATTTC ACAGACGCTG 
ATGGTCCTTC TCAAACGACT TCCTCCTAAG GGGAAGAAAC TTCTCGTCTT TGGGACAACA 
AGTGAAGTAA CCTTCTTAGA GTCTGTTGGT ATCTCTGATT GCTTCTCTGT CACTCACTCT 
GTTCCGACAC TGCAAAAAGA GGACGCAAAG AAGGTGCTAA ATCAGCTTAA CCTCTTCTCA 
GAGGATGATG TTGATTCAGC TGCAGAGGCT CTCAATGACA TGCCAATCAA GAAGATTTAC 
ATGCTAATAG AAATGGCTGC GCAAGGAGAA AACGGTGGAT CTGCAGAAGC TATCTATGCT 
GGGAGAGAGA AAATCAATAT CAATCATTTC TATGATTGCT TAGGAGACTT CATTCGCTTC 
ACTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192400.2
CDS	198..2426
Translation	MAGRYGSQVMTMTVTNTPSADLAFTNLAYCSSSDLRQFSVPGSDLFLANVADSFILSLCGHGSIRDGNIALNAIQRRHARVSTGDMVSVSRFVPPENFDLAMLTLELEFVKKGTKSEQVDAALLSTQLKRKYTNQVLTVGQKATFEYHGTNYILTVNRADVEGQDHTNGIERGLLSKDTYIVFEASNASGIKIVNQREAASSNIFKHKEFNLESLGIGGLGAEFADIFRRAFASRVFPPHVTSRLGIKHVKGMLLFGPPGTGKTLMARQIGKMLNGKDPKIVNGPEVLSKFVGETEKNVRDLFADAEQDQRTLGDASELHVIIFDEIDAICKSRGSTRDGTGVHDSIVNQLLTKIDGVEALNNVLLIGMTNRKDLLDEALLRPGRLEVQVEISLPDEAGRLQILQIHTNKMKENSFLGTDINLQELAARTKNYSGAELEGVVKSATSYALNRQLSMDDLTKPVEEENIKITMEDFLHAIYEVQPAFGASTDDLERCRLNGMVDCGHRHNHIYKRAMLLVEQVKVSTRSPLVTCLLEGPSGSGKTALAATIGIDSDFPYVKIVSAETMIGLSESTKCAHIVKVFEDAYKSPMSIIILDDIERLLEFIAIGPRFSNIISQTLMVLLKRLPPKGKKLLVFGTTSEVTFLESVGISDCFSVTHSVPTLQKEDAKKVLNQLNLFSEDDVDSAAEALNDMPIKKIYMLIEMAAQGENGGSAEAIYAGREKININHFYDCLGDFIRFTG

Target ORF information:

RefSeq Version	NM_116729.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana AAA-type ATPase family protein (NSF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_116729.4

ORF Insert Sequence:

ATGGCGGGTC GTTACGGATC GCAAGTCATG ACGATGACTG TGACCAATAC TCCGTCCGCA 
GATCTAGCTT TTACTAACCT CGCTTACTGC TCTTCTTCCG ATCTCCGTCA ATTCTCCGTT 
CCCGGTTCCG ATCTCTTCCT CGCCAACGTT GCCGATAGCT TCATTCTTTC TCTCTGTGGA 
CATGGAAGCA TCCGTGATGG AAATATTGCT CTCAATGCTA TTCAAAGGCG ACACGCTAGA 
GTTTCGACTG GTGATATGGT ATCTGTTAGC AGATTTGTTC CTCCTGAAAA TTTTGATCTG 
GCTATGCTGA CGCTGGAGTT GGAATTTGTG AAGAAAGGAA CTAAAAGCGA GCAGGTTGAT 
GCTGCTCTCC TTTCGACCCA GCTTAAGAGG AAATACACCA ACCAGGTCTT GACTGTAGGC 
CAGAAAGCGA CATTTGAGTA TCATGGAACT AATTACATTC TTACGGTCAA TCGAGCGGAT 
GTTGAGGGTC AGGATCACAC GAACGGCATT GAAAGAGGGT TGCTCTCTAA AGACACGTAT 
ATTGTGTTTG AAGCATCAAA TGCAAGTGGC ATAAAGATTG TCAACCAACG GGAAGCTGCT 
AGCAGCAATA TATTCAAACA TAAGGAATTT AACCTTGAGT CGCTGGGAAT AGGTGGTCTT 
GGTGCAGAGT TTGCTGATAT ATTTAGAAGA GCTTTTGCTT CTCGTGTATT TCCTCCTCAT 
GTCACCAGCA GACTGGGAAT AAAGCATGTT AAAGGGATGC TTCTTTTTGG ACCTCCTGGT 
ACTGGCAAAA CCCTTATGGC ACGGCAAATT GGAAAGATGC TTAATGGAAA GGATCCAAAG 
ATCGTTAATG GGCCTGAGGT GCTGAGCAAG TTTGTTGGTG AGACAGAAAA GAATGTAAGA 
GACTTATTTG CTGATGCTGA GCAAGACCAG AGGACCCTTG GTGATGCTAG TGAGTTGCAT 
GTCATTATTT TTGATGAGAT TGATGCTATT TGCAAGTCAA GAGGTTCAAC CAGAGATGGC 
ACAGGTGTCC ATGATAGTAT TGTAAACCAG CTTTTGACAA AGATAGATGG TGTGGAGGCC 
TTAAACAATG TTTTGCTGAT TGGAATGACA AACAGAAAGG ATTTGCTTGA TGAAGCTCTT 
TTAAGGCCTG GAAGATTGGA GGTTCAGGTT GAGATAAGCC TTCCTGATGA AGCTGGGCGT 
CTACAAATTC TTCAGATTCA TACAAATAAG ATGAAGGAAA ATTCTTTCCT TGGGACTGAT 
ATCAATCTCC AAGAGCTTGC TGCTCGAACA AAAAATTACA GTGGTGCAGA GCTTGAAGGT 
GTTGTTAAAA GTGCTACATC ATATGCATTA AACAGACAAC TAAGCATGGA TGATCTTACA 
AAGCCTGTGG AAGAAGAGAA TATCAAGATC ACTATGGAGG ATTTTCTCCA CGCTATCTAT 
GAAGTTCAAC CTGCATTTGG AGCCTCCACA GATGACCTAG AGCGCTGCAG ACTCAATGGG 
ATGGTAGATT GTGGTCATCG GCATAATCAC ATTTACAAAA GAGCTATGCT TCTAGTCGAG 
CAAGTTAAAG TTAGTACTAG AAGTCCTCTA GTCACTTGCC TTCTGGAGGG GCCCAGTGGA 
AGTGGGAAAA CTGCTTTAGC AGCAACAATT GGTATCGATA GTGACTTCCC ATATGTTAAG 
ATAGTTTCTG CGGAAACTAT GATTGGTCTT TCTGAGAGCA CAAAATGTGC TCACATTGTC 
AAGGTCTTTG AGGATGCATA TAAATCTCCA ATGAGCATCA TTATCCTTGA CGATATTGAA 
AGATTGTTGG AATTCATAGC AATCGGTCCT CGCTTTTCAA ACATAATTTC ACAGACGCTG 
ATGGTCCTTC TCAAACGACT TCCTCCTAAG GGGAAGAAAC TTCTCGTCTT TGGGACAACA 
AGTGAAGTAA CCTTCTTAGA GTCTGTTGGT ATCTCTGATT GCTTCTCTGT CACTCACTCT 
GTTCCGACAC TGCAAAAAGA GGACGCAAAG AAGGTGCTAA ATCAGCTTAA CCTCTTCTCA 
GAGGATGATG TTGATTCAGC TGCAGAGGCT CTCAATGACA TGCCAATCAA GAAGATTTAC 
ATGCTAATAG AAATGGCTGC GCAAGGAGAA AACGGTGGAT CTGCAGAAGC TATCTATGCT 
GGGAGAGAGA AAATCAATAT CAATCATTTC TATGATTGCT TAGGAGACTT CATTCGCTTC 
ACTGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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NSF cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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