NSFB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSFB
Entrez Gene ID	554200
Full Name	N-ethylmaleimide-sensitive factor b
General protein information	Preferred Names N-ethylmaleimide-sensitive factor b Names N-ethylmaleimide-sensitive factor b unm_au13 unm_au18
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156207.3	XP_005156264.1	N-ethylmaleimide-sensitive factor b isoform X1
XM_005156207.4	XP_005156264.1	N-ethylmaleimide-sensitive factor b isoform X1
XM_005156208.3	XP_005156265.1	N-ethylmaleimide-sensitive factor b isoform X1
XM_017358461.2	XP_017213950.1	N-ethylmaleimide-sensitive factor b isoform X2
XM_017358461.1	XP_017213950.1	N-ethylmaleimide-sensitive factor b isoform X2
NM_001024454.2	NP_001019625.2	N-ethylmaleimide-sensitive factor b
NM_001354163.1	NP_001341092.1	N-ethylmaleimide-sensitive factor b
NM_001024454.1	NP_001019625.1	N-ethylmaleimide-sensitive factor b

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	NSF	NP_192400.2
Oryza sativa (rice)	Os05g0519400	NP_001056063.1
Danio rerio (zebrafish)	nsfb	NP_001019625.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Bouwmeester MC, Ruiter S, Lommelaars T, Sippel J, Hodemaekers HM, van den Brandhof EJ, Pennings JL, Kamstra JH, Jelinek J, Issa JP, Legler J, van der Ven LT
Toxicology and applied pharmacology29184-96(2016 Jan)

N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium.
Hanovice NJ, Daly CM, Gross JM
Investigative ophthalmology & visual science56(12)7535-44(2015 Nov)

The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects.
Nishiwaki Y, Yoshizawa A, Kojima Y, Oguri E, Nakamura S, Suzuki S, Yuasa-Kawada J, Kinoshita-Kawada M, Mochizuki T, Masai I
Developmental cell25(4)374-87(2013 May)

An ENU mutagenesis screen in zebrafish for visual system mutants identifies a novel splice-acceptor site mutation in patched2 that results in Colobomas.
Lee J, Cox BD, Daly CM, Lee C, Nuckels RJ, Tittle RK, Uribe RA, Gross JM
Investigative ophthalmology & visual science53(13)8214-21(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NSFB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSFB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa42083	XM_017358461.2 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa17870	NM_001354163.1 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17870	NM_001024454.2 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa37224	NM_001024454.1 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17870	XM_005156208.3 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa17870	XM_005156207.4 Latest version!	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa17870	XM_005156207.3	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa42083	XM_017358461.1	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSFB ( XM_017358461.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213950.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42083
Clone ID Related Accession (Same CDS sequence)	XM_017358461.2 , XM_017358461.1
Accession Version	XM_017358461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	N-ethylmaleimide-sensitive factor b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATCG AGATCGACTT CCTCCAGAAA AAGAGCATTG ACAGCAATCC TTATGACTCT 
GATAAAATGG CCATTGAATT TATTCAGTGT TTCACTGCAC AGGCCTTTAG CATGAGTCAA 
CAGTTTGTCT TTAGTTTTTG TGACAAACTC TTTGCGCTGG TCATAAAAGA TATTGAGGCC 
ATGGATCCCA GCATTCTCAG AGGCGAGCAG AACTCTAGCA AAAAACACAA GATTGACATT 
GGTCTACTGC ATGGCAATAG TCAGGTGATT TTTGAGAAAG CGGAGAGCTC GTCTGTCAAT 
TTGATTGGAA AAGCAAAAAC CAAAGGCTCT CGTCAGTCTA TAATCAACCC AGACTGGAAC 
TTTGAGCGCA TGGGCATCGG GGGCCTGGAC AAGGAATTCT CAGATATCTT CCGTCGGGCT 
TTTGCTTCAC GAGTCTTTCC TCCAGACATA GTAGAACAAA TGGGTTGCAA GCACGTGAAG 
GGAATCTTGC TGTATGGACC ACCCGGCTGT GGTAAAACCC TAATGGCACG GCAGATTGGA 
AAGATGCTGA ACGCCAGAGA GCCAAAAGTG GTCAACGGGC CAGAGATTTT GAACAAATAC 
GTGGGAGAAT CGGAGGCCAA CATCAGAAAG CTATTCGCTG ATGCTGAGGA GGAACAGAAG 
AGGTTGGGGG CCAACAGCGG CCTGCACATC ATTATATTTG ACGAAATTGA TGCCATTTGC 
AAACAACGTG GCAGCATGGC AGGCAGCACA GGGGTCCATG ACACTGTAGT GAACCAGCTT 
CTGTCCAAGA TCGATGGTGT AGAGCAGCTC AACAACATCC TGGTTATTGG CATGACCAAC 
AGGCCTGACC TGATTGATGA AGCTCTATTG AGGCCGGGTA GATTGGAAGT CAAAATGGAG 
ATTGGCTTGC CTGATGAGAC GGGTCGTGTT CAGATCCTAA ATATCCACAC GGCAAAGATG 
AAGCAGTCAA ACATGCTGGC CAAAGACGTT GACGTAAAGG AACTGGCTGT CGAGACCAAG 
AACTACAGTG GAGCAGAGCT GGAAGGGCTT GTTCGGGCTG CGCAGTCCAC TGCCATGAAC 
CGTCACATCA AGGCCACTAC TCAGGTAGAG GTGGACACAG AAAAGGCTCA GACTCTGCAA 
GTCAGCAGGA GTGACTTCTT GGCATCTCTG AATAATGACA TAAAGCCTGC ATTTGGTAGT 
AATCAGGAGG ATTACTCCAG TTACATCATG AACGGTATTG TGAAATGGAG CAATGCTGTG 
TCTGACATAC TGGGAGACGG AGAACTTCTG GTGCAGCAGA CCAAGAACAG TGAACGCACA 
CCCCTAGTTA CTGTTCTATT AGAGGGGCCT GCTCACAGTG GTAAGACCGC ACTGGCGGCC 
AAGATTGCAG AGCAGTCCCA GTTCCCCTTC ATCAAGATCT GCTCTCCAGA CAAAATGATC 
GGCTTTACAG AGACCGCCAA ATGTCAGGCC ATAAAGAAGA TATTTGAGGA TGCCTACAAG 
TCTCAGTTGA GCTGTGTGGT GGTGGACGAT ATTGAGCGAC TGCTGGATTT TGTTCCCATC 
GGGCCTCGGT TCTCTAACCT GGTGCTGCAG GCTCTGCTGG TGTTACTGAA GAAACCTCCT 
CCTCGGGGTC GTAAGTTGTT GATTCTTGGC ACAACTAGCA GAAAGGATGT CCTGCAGGAG 
ATGGGCATGT CCGACTCTTT CAGCACTACA ATCCACATCC CCAACATCTC TAGAGGAGAA 
CATCTAATGG AGGCCCTGGA GCTTCTTGGA GGCTTTCAGG AAGAAGAGCG AGCGAATATT 
GGCAAACAGG TGAAAGGACA GAGCGTGTGG CTGGGCATTA AAAAGCTGCA AATGCTGATT 
GAGATGTCAC TGCAGATGCC CCTGGAATAC AGAGTTCAGA AGTTTTTGGC ACTGCTGAAG 
GAAGAAGGAG CGCTTGGCCC TGATAAACAT GTGGCTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213950.1
CDS	186..2147
Translation	MTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	XM_017358461.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358461.2

ORF Insert Sequence:

ATGACAATCG AGATCGACTT CCTCCAGAAA AAGAGCATTG ACAGCAATCC TTATGACTCT 
GATAAAATGG CCATTGAATT TATTCAGTGT TTCACTGCAC AGGCCTTTAG CATGAGTCAA 
CAGTTTGTCT TTAGTTTTTG TGACAAACTC TTTGCGCTGG TCATAAAAGA TATTGAGGCC 
ATGGATCCCA GCATTCTCAG AGGCGAGCAG AACTCTAGCA AAAAACACAA GATTGACATT 
GGTCTACTGC ATGGCAATAG TCAGGTGATT TTTGAGAAAG CGGAGAGCTC GTCTGTCAAT 
TTGATTGGAA AAGCAAAAAC CAAAGGCTCT CGTCAGTCTA TAATCAACCC AGACTGGAAC 
TTTGAGCGCA TGGGCATCGG GGGCCTGGAC AAGGAATTCT CAGATATCTT CCGTCGGGCT 
TTTGCTTCAC GAGTCTTTCC TCCAGACATA GTAGAACAAA TGGGTTGCAA GCACGTGAAG 
GGAATCTTGC TGTATGGACC ACCCGGCTGT GGTAAAACCC TAATGGCACG GCAGATTGGA 
AAGATGCTGA ACGCCAGAGA GCCAAAAGTG GTCAACGGGC CAGAGATTTT GAACAAATAC 
GTGGGAGAAT CGGAGGCCAA CATCAGAAAG CTATTCGCTG ATGCTGAGGA GGAACAGAAG 
AGGTTGGGGG CCAACAGCGG CCTGCACATC ATTATATTTG ACGAAATTGA TGCCATTTGC 
AAACAACGTG GCAGCATGGC AGGCAGCACA GGGGTCCATG ACACTGTAGT GAACCAGCTT 
CTGTCCAAGA TCGATGGTGT AGAGCAGCTC AACAACATCC TGGTTATTGG CATGACCAAC 
AGGCCTGACC TGATTGATGA AGCTCTATTG AGGCCGGGTA GATTGGAAGT CAAAATGGAG 
ATTGGCTTGC CTGATGAGAC GGGTCGTGTT CAGATCCTAA ATATCCACAC GGCAAAGATG 
AAGCAGTCAA ACATGCTGGC CAAAGACGTT GACGTAAAGG AACTGGCTGT CGAGACCAAG 
AACTACAGTG GAGCAGAGCT GGAAGGGCTT GTTCGGGCTG CGCAGTCCAC TGCCATGAAC 
CGTCACATCA AGGCCACTAC TCAGGTAGAG GTGGACACAG AAAAGGCTCA GACTCTGCAA 
GTCAGCAGGA GTGACTTCTT GGCATCTCTG AATAATGACA TAAAGCCTGC ATTTGGTAGT 
AATCAGGAGG ATTACTCCAG TTACATCATG AACGGTATTG TGAAATGGAG CAATGCTGTG 
TCTGACATAC TGGGAGACGG AGAACTTCTG GTGCAGCAGA CCAAGAACAG TGAACGCACA 
CCCCTAGTTA CTGTTCTATT AGAGGGGCCT GCTCACAGTG GTAAGACCGC ACTGGCGGCC 
AAGATTGCAG AGCAGTCCCA GTTCCCCTTC ATCAAGATCT GCTCTCCAGA CAAAATGATC 
GGCTTTACAG AGACCGCCAA ATGTCAGGCC ATAAAGAAGA TATTTGAGGA TGCCTACAAG 
TCTCAGTTGA GCTGTGTGGT GGTGGACGAT ATTGAGCGAC TGCTGGATTT TGTTCCCATC 
GGGCCTCGGT TCTCTAACCT GGTGCTGCAG GCTCTGCTGG TGTTACTGAA GAAACCTCCT 
CCTCGGGGTC GTAAGTTGTT GATTCTTGGC ACAACTAGCA GAAAGGATGT CCTGCAGGAG 
ATGGGCATGT CCGACTCTTT CAGCACTACA ATCCACATCC CCAACATCTC TAGAGGAGAA 
CATCTAATGG AGGCCCTGGA GCTTCTTGGA GGCTTTCAGG AAGAAGAGCG AGCGAATATT 
GGCAAACAGG TGAAAGGACA GAGCGTGTGG CTGGGCATTA AAAAGCTGCA AATGCTGATT 
GAGATGTCAC TGCAGATGCC CCTGGAATAC AGAGTTCAGA AGTTTTTGGC ACTGCTGAAG 
GAAGAAGGAG CGCTTGGCCC TGATAAACAT GTGGCTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( XM_017358461.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213950.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa42083
Clone ID Related Accession (Same CDS sequence)	XM_017358461.2 , XM_017358461.1
Accession Version	XM_017358461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-ethylmaleimide-sensitive factor b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATCG AGATCGACTT CCTCCAGAAA AAGAGCATTG ACAGCAATCC TTATGACTCT 
GATAAAATGG CCATTGAATT TATTCAGTGT TTCACTGCAC AGGCCTTTAG CATGAGTCAA 
CAGTTTGTCT TTAGTTTTTG TGACAAACTC TTTGCGCTGG TCATAAAAGA TATTGAGGCC 
ATGGATCCCA GCATTCTCAG AGGCGAGCAG AACTCTAGCA AAAAACACAA GATTGACATT 
GGTCTACTGC ATGGCAATAG TCAGGTGATT TTTGAGAAAG CGGAGAGCTC GTCTGTCAAT 
TTGATTGGAA AAGCAAAAAC CAAAGGCTCT CGTCAGTCTA TAATCAACCC AGACTGGAAC 
TTTGAGCGCA TGGGCATCGG GGGCCTGGAC AAGGAATTCT CAGATATCTT CCGTCGGGCT 
TTTGCTTCAC GAGTCTTTCC TCCAGACATA GTAGAACAAA TGGGTTGCAA GCACGTGAAG 
GGAATCTTGC TGTATGGACC ACCCGGCTGT GGTAAAACCC TAATGGCACG GCAGATTGGA 
AAGATGCTGA ACGCCAGAGA GCCAAAAGTG GTCAACGGGC CAGAGATTTT GAACAAATAC 
GTGGGAGAAT CGGAGGCCAA CATCAGAAAG CTATTCGCTG ATGCTGAGGA GGAACAGAAG 
AGGTTGGGGG CCAACAGCGG CCTGCACATC ATTATATTTG ACGAAATTGA TGCCATTTGC 
AAACAACGTG GCAGCATGGC AGGCAGCACA GGGGTCCATG ACACTGTAGT GAACCAGCTT 
CTGTCCAAGA TCGATGGTGT AGAGCAGCTC AACAACATCC TGGTTATTGG CATGACCAAC 
AGGCCTGACC TGATTGATGA AGCTCTATTG AGGCCGGGTA GATTGGAAGT CAAAATGGAG 
ATTGGCTTGC CTGATGAGAC GGGTCGTGTT CAGATCCTAA ATATCCACAC GGCAAAGATG 
AAGCAGTCAA ACATGCTGGC CAAAGACGTT GACGTAAAGG AACTGGCTGT CGAGACCAAG 
AACTACAGTG GAGCAGAGCT GGAAGGGCTT GTTCGGGCTG CGCAGTCCAC TGCCATGAAC 
CGTCACATCA AGGCCACTAC TCAGGTAGAG GTGGACACAG AAAAGGCTCA GACTCTGCAA 
GTCAGCAGGA GTGACTTCTT GGCATCTCTG AATAATGACA TAAAGCCTGC ATTTGGTAGT 
AATCAGGAGG ATTACTCCAG TTACATCATG AACGGTATTG TGAAATGGAG CAATGCTGTG 
TCTGACATAC TGGGAGACGG AGAACTTCTG GTGCAGCAGA CCAAGAACAG TGAACGCACA 
CCCCTAGTTA CTGTTCTATT AGAGGGGCCT GCTCACAGTG GTAAGACCGC ACTGGCGGCC 
AAGATTGCAG AGCAGTCCCA GTTCCCCTTC ATCAAGATCT GCTCTCCAGA CAAAATGATC 
GGCTTTACAG AGACCGCCAA ATGTCAGGCC ATAAAGAAGA TATTTGAGGA TGCCTACAAG 
TCTCAGTTGA GCTGTGTGGT GGTGGACGAT ATTGAGCGAC TGCTGGATTT TGTTCCCATC 
GGGCCTCGGT TCTCTAACCT GGTGCTGCAG GCTCTGCTGG TGTTACTGAA GAAACCTCCT 
CCTCGGGGTC GTAAGTTGTT GATTCTTGGC ACAACTAGCA GAAAGGATGT CCTGCAGGAG 
ATGGGCATGT CCGACTCTTT CAGCACTACA ATCCACATCC CCAACATCTC TAGAGGAGAA 
CATCTAATGG AGGCCCTGGA GCTTCTTGGA GGCTTTCAGG AAGAAGAGCG AGCGAATATT 
GGCAAACAGG TGAAAGGACA GAGCGTGTGG CTGGGCATTA AAAAGCTGCA AATGCTGATT 
GAGATGTCAC TGCAGATGCC CCTGGAATAC AGAGTTCAGA AGTTTTTGGC ACTGCTGAAG 
GAAGAAGGAG CGCTTGGCCC TGATAAACAT GTGGCTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213950.1
CDS	186..2147
Translation	MTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	XM_017358461.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358461.1

ORF Insert Sequence:

ATGACAATCG AGATCGACTT CCTCCAGAAA AAGAGCATTG ACAGCAATCC TTATGACTCT 
GATAAAATGG CCATTGAATT TATTCAGTGT TTCACTGCAC AGGCCTTTAG CATGAGTCAA 
CAGTTTGTCT TTAGTTTTTG TGACAAACTC TTTGCGCTGG TCATAAAAGA TATTGAGGCC 
ATGGATCCCA GCATTCTCAG AGGCGAGCAG AACTCTAGCA AAAAACACAA GATTGACATT 
GGTCTACTGC ATGGCAATAG TCAGGTGATT TTTGAGAAAG CGGAGAGCTC GTCTGTCAAT 
TTGATTGGAA AAGCAAAAAC CAAAGGCTCT CGTCAGTCTA TAATCAACCC AGACTGGAAC 
TTTGAGCGCA TGGGCATCGG GGGCCTGGAC AAGGAATTCT CAGATATCTT CCGTCGGGCT 
TTTGCTTCAC GAGTCTTTCC TCCAGACATA GTAGAACAAA TGGGTTGCAA GCACGTGAAG 
GGAATCTTGC TGTATGGACC ACCCGGCTGT GGTAAAACCC TAATGGCACG GCAGATTGGA 
AAGATGCTGA ACGCCAGAGA GCCAAAAGTG GTCAACGGGC CAGAGATTTT GAACAAATAC 
GTGGGAGAAT CGGAGGCCAA CATCAGAAAG CTATTCGCTG ATGCTGAGGA GGAACAGAAG 
AGGTTGGGGG CCAACAGCGG CCTGCACATC ATTATATTTG ACGAAATTGA TGCCATTTGC 
AAACAACGTG GCAGCATGGC AGGCAGCACA GGGGTCCATG ACACTGTAGT GAACCAGCTT 
CTGTCCAAGA TCGATGGTGT AGAGCAGCTC AACAACATCC TGGTTATTGG CATGACCAAC 
AGGCCTGACC TGATTGATGA AGCTCTATTG AGGCCGGGTA GATTGGAAGT CAAAATGGAG 
ATTGGCTTGC CTGATGAGAC GGGTCGTGTT CAGATCCTAA ATATCCACAC GGCAAAGATG 
AAGCAGTCAA ACATGCTGGC CAAAGACGTT GACGTAAAGG AACTGGCTGT CGAGACCAAG 
AACTACAGTG GAGCAGAGCT GGAAGGGCTT GTTCGGGCTG CGCAGTCCAC TGCCATGAAC 
CGTCACATCA AGGCCACTAC TCAGGTAGAG GTGGACACAG AAAAGGCTCA GACTCTGCAA 
GTCAGCAGGA GTGACTTCTT GGCATCTCTG AATAATGACA TAAAGCCTGC ATTTGGTAGT 
AATCAGGAGG ATTACTCCAG TTACATCATG AACGGTATTG TGAAATGGAG CAATGCTGTG 
TCTGACATAC TGGGAGACGG AGAACTTCTG GTGCAGCAGA CCAAGAACAG TGAACGCACA 
CCCCTAGTTA CTGTTCTATT AGAGGGGCCT GCTCACAGTG GTAAGACCGC ACTGGCGGCC 
AAGATTGCAG AGCAGTCCCA GTTCCCCTTC ATCAAGATCT GCTCTCCAGA CAAAATGATC 
GGCTTTACAG AGACCGCCAA ATGTCAGGCC ATAAAGAAGA TATTTGAGGA TGCCTACAAG 
TCTCAGTTGA GCTGTGTGGT GGTGGACGAT ATTGAGCGAC TGCTGGATTT TGTTCCCATC 
GGGCCTCGGT TCTCTAACCT GGTGCTGCAG GCTCTGCTGG TGTTACTGAA GAAACCTCCT 
CCTCGGGGTC GTAAGTTGTT GATTCTTGGC ACAACTAGCA GAAAGGATGT CCTGCAGGAG 
ATGGGCATGT CCGACTCTTT CAGCACTACA ATCCACATCC CCAACATCTC TAGAGGAGAA 
CATCTAATGG AGGCCCTGGA GCTTCTTGGA GGCTTTCAGG AAGAAGAGCG AGCGAATATT 
GGCAAACAGG TGAAAGGACA GAGCGTGTGG CTGGGCATTA AAAAGCTGCA AATGCTGATT 
GAGATGTCAC TGCAGATGCC CCTGGAATAC AGAGTTCAGA AGTTTTTGGC ACTGCTGAAG 
GAAGAAGGAG CGCTTGGCCC TGATAAACAT GTGGCTTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( NM_001024454.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001019625.2 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa17870
Clone ID Related Accession (Same CDS sequence)	XM_005156207.3 , XM_005156207.4 , XM_005156208.3 , NM_001024454.2 , NM_001354163.1
Accession Version	NM_001024454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	N-ethylmaleimide-sensitive factor b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929346.6 and CU570973.4. On Aug 8, 2017 this sequence version replaced NM_001024454.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: DQ022212.1, BC162793.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019625.2
CDS	57..2300
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	NM_001024454.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024454.2

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( NM_001354163.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001341092.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 2, mRNA.

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CloneID	ODa17870
Clone ID Related Accession (Same CDS sequence)	XM_005156207.3 , XM_005156207.4 , XM_005156208.3 , NM_001024454.2 , NM_001354163.1
Accession Version	NM_001354163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	N-ethylmaleimide-sensitive factor b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929346.6 and CU570973.4. On Aug 8, 2017 this sequence version replaced XM_005156208.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01028766.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341092.1
CDS	57..2300
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	NM_001354163.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354163.1

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( NM_001024454.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001019625.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), mRNA.

Price & Availability↑

CloneID	ODa37224
Clone ID Related Accession (Same CDS sequence)	NM_001024454.1
Accession Version	NM_001024454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(Zebrafish)
Product	N-ethylmaleimide-sensitive factor b
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ022212.1. On Sep 16, 2005 this sequence version replaced XM_686914.1. ##Evidence-Data-START## Transcript exon combination :: DQ022212.1, BC162793.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTGG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTTTGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT CTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGATGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTTTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019625.1
CDS	57..2300
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLFGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	NM_001024454.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024454.1

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTGG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTTTGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT CTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGATGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTTTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( XM_005156207.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156264.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17870
Clone ID Related Accession (Same CDS sequence)	XM_005156207.3 , XM_005156207.4 , XM_005156208.3 , NM_001024454.2 , NM_001354163.1
Accession Version	XM_005156207.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-ethylmaleimide-sensitive factor b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156207.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156264.1
CDS	92..2335
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	XM_005156207.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156207.3

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( XM_005156207.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156264.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa17870
Clone ID Related Accession (Same CDS sequence)	XM_005156207.3 , XM_005156207.4 , XM_005156208.3 , NM_001024454.2 , NM_001354163.1
Accession Version	XM_005156207.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	N-ethylmaleimide-sensitive factor b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156207.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156264.1
CDS	94..2337
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	XM_005156207.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156207.4

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSFB ( XM_005156208.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005156265.1 Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X2, mRNA.

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CloneID	ODa17870
Clone ID Related Accession (Same CDS sequence)	XM_005156207.3 , XM_005156207.4 , XM_005156208.3 , NM_001024454.2 , NM_001354163.1
Accession Version	XM_005156208.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	N-ethylmaleimide-sensitive factor b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156265.1
CDS	92..2335
Translation	MAGRQMQAARCPTDELSLTNCVVASEKDFKSGQHLTIKTTPTQKFVFTVRTHPSVVPGTIAFSLPQRKWAGLSLNQDVEVSVYNFDPSRQYVGTMTIEIDFLQKKSIDSNPYDSDKMAIEFIQCFTAQAFSMSQQFVFSFCDKLFALVIKDIEAMDPSILRGEQNSSKKHKIDIGLLHGNSQVIFEKAESSSVNLIGKAKTKGSRQSIINPDWNFERMGIGGLDKEFSDIFRRAFASRVFPPDIVEQMGCKHVKGILLYGPPGCGKTLMARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQKRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDETGRVQILNIHTAKMKQSNMLAKDVDVKELAVETKNYSGAELEGLVRAAQSTAMNRHIKATTQVEVDTEKAQTLQVSRSDFLASLNNDIKPAFGSNQEDYSSYIMNGIVKWSNAVSDILGDGELLVQQTKNSERTPLVTVLLEGPAHSGKTALAAKIAEQSQFPFIKICSPDKMIGFTETAKCQAIKKIFEDAYKSQLSCVVVDDIERLLDFVPIGPRFSNLVLQALLVLLKKPPPRGRKLLILGTTSRKDVLQEMGMSDSFSTTIHIPNISRGEHLMEALELLGGFQEEERANIGKQVKGQSVWLGIKKLQMLIEMSLQMPLEYRVQKFLALLKEEGALGPDKHVAF

Target ORF information:

RefSeq Version	XM_005156208.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio N-ethylmaleimide-sensitive factor b (nsfb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156208.3

ORF Insert Sequence:

ATGGCTGGCC GACAAATGCA AGCAGCGAGA TGCCCAACAG ATGAACTGTC TCTCACAAAC 
TGTGTGGTTG CAAGTGAGAA AGACTTCAAA TCTGGGCAGC ATTTGACTAT TAAAACCACT 
CCAACTCAGA AATTTGTTTT CACTGTGAGA ACTCATCCAA GCGTGGTGCC AGGAACCATT 
GCCTTCAGTC TACCTCAGAG AAAATGGGCT GGACTCTCTC TAAACCAAGA TGTGGAAGTG 
AGTGTCTACA ACTTTGACCC GTCCAGACAG TATGTGGGAA CCATGACAAT CGAGATCGAC 
TTCCTCCAGA AAAAGAGCAT TGACAGCAAT CCTTATGACT CTGATAAAAT GGCCATTGAA 
TTTATTCAGT GTTTCACTGC ACAGGCCTTT AGCATGAGTC AACAGTTTGT CTTTAGTTTT 
TGTGACAAAC TCTTTGCGCT GGTCATAAAA GATATTGAGG CCATGGATCC CAGCATTCTC 
AGAGGCGAGC AGAACTCTAG CAAAAAACAC AAGATTGACA TTGGTCTACT GCATGGCAAT 
AGTCAGGTGA TTTTTGAGAA AGCGGAGAGC TCGTCTGTCA ATTTGATTGG AAAAGCAAAA 
ACCAAAGGCT CTCGTCAGTC TATAATCAAC CCAGACTGGA ACTTTGAGCG CATGGGCATC 
GGGGGCCTGG ACAAGGAATT CTCAGATATC TTCCGTCGGG CTTTTGCTTC ACGAGTCTTT 
CCTCCAGACA TAGTAGAACA AATGGGTTGC AAGCACGTGA AGGGAATCTT GCTGTATGGA 
CCACCCGGCT GTGGTAAAAC CCTAATGGCA CGGCAGATTG GAAAGATGCT GAACGCCAGA 
GAGCCAAAAG TGGTCAACGG GCCAGAGATT TTGAACAAAT ACGTGGGAGA ATCGGAGGCC 
AACATCAGAA AGCTATTCGC TGATGCTGAG GAGGAACAGA AGAGGTTGGG GGCCAACAGC 
GGCCTGCACA TCATTATATT TGACGAAATT GATGCCATTT GCAAACAACG TGGCAGCATG 
GCAGGCAGCA CAGGGGTCCA TGACACTGTA GTGAACCAGC TTCTGTCCAA GATCGATGGT 
GTAGAGCAGC TCAACAACAT CCTGGTTATT GGCATGACCA ACAGGCCTGA CCTGATTGAT 
GAAGCTCTAT TGAGGCCGGG TAGATTGGAA GTCAAAATGG AGATTGGCTT GCCTGATGAG 
ACGGGTCGTG TTCAGATCCT AAATATCCAC ACGGCAAAGA TGAAGCAGTC AAACATGCTG 
GCCAAAGACG TTGACGTAAA GGAACTGGCT GTCGAGACCA AGAACTACAG TGGAGCAGAG 
CTGGAAGGGC TTGTTCGGGC TGCGCAGTCC ACTGCCATGA ACCGTCACAT CAAGGCCACT 
ACTCAGGTAG AGGTGGACAC AGAAAAGGCT CAGACTCTGC AAGTCAGCAG GAGTGACTTC 
TTGGCATCTC TGAATAATGA CATAAAGCCT GCATTTGGTA GTAATCAGGA GGATTACTCC 
AGTTACATCA TGAACGGTAT TGTGAAATGG AGCAATGCTG TGTCTGACAT ACTGGGAGAC 
GGAGAACTTC TGGTGCAGCA GACCAAGAAC AGTGAACGCA CACCCCTAGT TACTGTTCTA 
TTAGAGGGGC CTGCTCACAG TGGTAAGACC GCACTGGCGG CCAAGATTGC AGAGCAGTCC 
CAGTTCCCCT TCATCAAGAT CTGCTCTCCA GACAAAATGA TCGGCTTTAC AGAGACCGCC 
AAATGTCAGG CCATAAAGAA GATATTTGAG GATGCCTACA AGTCTCAGTT GAGCTGTGTG 
GTGGTGGACG ATATTGAGCG ACTGCTGGAT TTTGTTCCCA TCGGGCCTCG GTTCTCTAAC 
CTGGTGCTGC AGGCTCTGCT GGTGTTACTG AAGAAACCTC CTCCTCGGGG TCGTAAGTTG 
TTGATTCTTG GCACAACTAG CAGAAAGGAT GTCCTGCAGG AGATGGGCAT GTCCGACTCT 
TTCAGCACTA CAATCCACAT CCCCAACATC TCTAGAGGAG AACATCTAAT GGAGGCCCTG 
GAGCTTCTTG GAGGCTTTCA GGAAGAAGAG CGAGCGAATA TTGGCAAACA GGTGAAAGGA 
CAGAGCGTGT GGCTGGGCAT TAAAAAGCTG CAAATGCTGA TTGAGATGTC ACTGCAGATG 
CCCCTGGAAT ACAGAGTTCA GAAGTTTTTG GCACTGCTGA AGGAAGAAGG AGCGCTTGGC 
CCTGATAAAC ATGTGGCTTT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zebrafish embryos as a screen for DNA methylation modifications after compound exposure.
Toxicology and applied pharmacology29184-96(2016 Jan)
Bouwmeester MC,Ruiter S,Lommelaars T,Sippel J,Hodemaekers HM,van den Brandhof EJ,Pennings JL,Kamstra JH,Jelinek J,Issa JP,Legler J,van der Ven LT

N-Ethylmaleimide-Sensitive Factor b (nsfb) Is Required for Normal Pigmentation of the Zebrafish Retinal Pigment Epithelium.
Investigative ophthalmology & visual science56(12)7535-44(2015 Nov)
Hanovice NJ,Daly CM,Gross JM

The BH3-only SNARE BNip1 mediates photoreceptor apoptosis in response to vesicular fusion defects.
Developmental cell25(4)374-87(2013 May)
Nishiwaki Y,Yoshizawa A,Kojima Y,Oguri E,Nakamura S,Suzuki S,Yuasa-Kawada J,Kinoshita-Kawada M,Mochizuki T,Masai I

An ENU mutagenesis screen in zebrafish for visual system mutants identifies a novel splice-acceptor site mutation in patched2 that results in Colobomas.
Investigative ophthalmology & visual science53(13)8214-21(2012 Dec)
Lee J,Cox BD,Daly CM,Lee C,Nuckels RJ,Tittle RK,Uribe RA,Gross JM

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NSFB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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