CHRNA7 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA7
Entrez Gene ID	282178
Full Name	cholinergic receptor, nicotinic, alpha 7
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174515.2	NP_776940.1	neuronal acetylcholine receptor subunit alpha-7 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chrna7	NP_031416.3
Rattus norvegicus (Norway rat)	Chrna7	NP_036964.3
Drosophila melanogaster (fruit fly)	nAcRalpha-34E	NP_001246027.1
Caenorhabditis elegans (roundworm)	acr-16	NP_505207.1
Caenorhabditis elegans (roundworm)	acr-25	NP_001023570.2
Caenorhabditis elegans (roundworm)	acr-7	NP_495647.1
Pan troglodytes (chimpanzee)	CHRNA7	XP_001164138.1
	CHRNA7	NP_001028055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008588	XP_314691.2
Caenorhabditis elegans (roundworm)	acr-11	NP_491906.1
Caenorhabditis elegans (roundworm)	acr-9	NP_510285.2
Caenorhabditis elegans (roundworm)	acr-14	NP_495716.1
Homo sapiens (human)	CHRNA7	NP_000737.1
Bos taurus (cattle)	CHRNA7	NP_776940.1
Gallus gallus (chicken)	CHRNA7	NP_989512.2
Caenorhabditis elegans (roundworm)	acr-10	NP_508692.2
Canis lupus familiaris (dog)	CHRNA7	XP_545813.4
Danio rerio (zebrafish)	chrna7	NP_957513.1
Xenopus tropicalis (tropical clawed frog)	LOC100493611	XP_002937717.2

Gene Ontology
Bibliography

Related articles in PubMed

Substitutions of amino acids in the pore domain of homomeric ?7 nicotinic receptors for analogous residues present in heteromeric receptors modify gating, rectification and binding properties.
Criado M, Svobodov? L, Mulet J, Sala F, Sala S
Journal of neurochemistry119(1)40-9(2011 Oct)

A small cytoplasmic region adjacent to the fourth transmembrane segment of the ?7 nicotinic receptor is essential for its biogenesis.
Criado M, Mulet J, Gerber S, Sala S, Sala F
FEBS letters585(15)2477-80(2011 Aug)

An extracellular RRR motif flanking the M1 transmembrane domain governs the biogenesis of homomeric neuronal nicotinic acetylcholine receptors.
Alves DS, Castello-Banyuls J, Faura CC, Ballesta JJ
FEBS letters585(8)1169-74(2011 Apr)

Evidence of alpha 7 nicotinic acetylcholine receptor expression in retinal pigment epithelial cells.
Maneu V, Gerona G, Fern?ndez L, Cuenca N, Lax P
Visual neuroscience27(5-6)139-47(2010 Nov)

Role of loop 9 on the function of neuronal nicotinic receptors.
Criado M, Castillo M, Mulet J, Sala F, Sala S
Biochimica et biophysica acta1798(3)654-9(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that residues in the M2 segment and flanking regions contribute to the unusual properties of the CHRNA7 receptor
Title: Substitutions of amino acids in the pore domain of homomeric ?7 nicotinic receptors for analogous residues present in heteromeric receptors modify gating, rectification and binding properties.

Deletion of a small cytoplasmic fragment close to the fourth transmembrane segment of the nicotinic alpha7 receptor (Glu437 to Arg447) abolished membrane expression
Title: A small cytoplasmic region adjacent to the fourth transmembrane segment of the ?7 nicotinic receptor is essential for its biogenesis.

Data show that basic residues at positions 208 and 210 are necessary for the assembly of alpha7 receptors.
Title: An extracellular RRR motif flanking the M1 transmembrane domain governs the biogenesis of homomeric neuronal nicotinic acetylcholine receptors.

Cultured bovine retinal pigment epitheliumcells expressed nicotinic receptors containing alpha7 subunit
Title: Evidence of alpha 7 nicotinic acetylcholine receptor expression in retinal pigment epithelial cells.

Loop 9 has a different role in the function of homomeric and heteromeric receptors.
Title: Role of loop 9 on the function of neuronal nicotinic receptors.

The following CHRNA7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41669	NM_174515.2 Latest version!	Bos taurus cholinergic receptor, nicotinic, alpha 7 (CHRNA7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA7 ( NM_174515.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776940.1 Bos taurus cholinergic receptor, nicotinic, alpha 7 (CHRNA7), mRNA.

Price & Availability↑

CloneID	OBa41669
Clone ID Related Accession (Same CDS sequence)	NM_174515.2
Accession Version	NM_174515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	neuronal acetylcholine receptor subunit alpha-7 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X93604.1. On Jun 3, 2003 this sequence version replaced NM_174515.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X93604.1, BC149340.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145497 [ECO:0000348] ##Evidence-Data-END##

ATGCGCGGCT CGTTGTGCCT GGCGCTGGCA GCGTCGATCT TGCACGTGTC CCTGCAAGGC 
GAGTTCCAGA GGAAGCTCTA CAAAGACCTG GTGAAGAACT ACAACCCCTT GGAGAGGCCG 
GTGGCCAACG ACTCGCTGCC GCTCACAGTC TACTTCTCCC TGAGCCTCCT GCAGATCATG 
GACGTGGATG AGAAGAACCA GGTGTTGACC ACCAACATTT GGCTACAAAT GACTTGGACA 
GATCACTATT TACAGTGGAA TGCATCCGAG TATCCAGGCG TGAAGACGGT TCGTTTCCCA 
GACGGCCAGA TCTGGAAGCC AGACATTCTC CTCTACAACA GTGCTGATGA ACGGTTTGAT 
GCTACGTTCC ACACCAATGT GTTAGTGAAT TCTTCTGGGC ACTGCCAGTA CCTCCCTCCA 
GGCATCTTTA AGAGCTCCTG CTACATCGAT GTGCGCTGGT TCCCATTTGA TGTGCAGCAG 
TGCAAACTGA AGTTTGGGTC CTGGTCTTAT GGAGGATGGT CCTTGGATCT GCAAATGCAG 
GAGGCAGACA TCAGCGGCTA TATCCCAAAT GGAGAATGGG ACCTTGTGGG GGTCTTGGGC 
AAGCGGAGTG AGAAGTTCTA CGAGTGCTGC AAAGAACCCT ACCCAGACGT GACCTTCACG 
GTCAGCATCC GCCGCAGGAC TCTCTACTAC GGCCTCAACC TGCTCATCCC CTGTGTGCTC 
ATCTCTGCCC TAGCCCTCCT CGTCTTCTTG CTCCCTGCAG ACTCTGGAGA GAAAATCTCC 
CTGGGAATCA CAGTTTTACT CTCCCTCACC GTCTTCATGC TGCTGGTAGC TGAGATCATG 
CCTGCGACCT CTGACTCAGT GCCCTTAATA GCCCAGTACT TCGCCAGCAC CATGATCATC 
GTGGGCCTCT CTGTGGTTGT CACGGTGATC GTGCTACAGT ACCACCACCA CGACCCTGAT 
GGTGGCAAGA TGCCCAAGTG GACCAGAGTC GTCCTCCTGA ACTGGTGTGC CTGGTTCCTT 
CGCATGAAGA GGCCGGGGGA GGACAAGGTG CGGCCAGCCT GCCAGCACAA CGAGCGGCGC 
TGCAGCCTGG CCAGCGTGGA GATGAGCGCA GTGGCGGGGC CGCCGGCCAC CAATGGCAAC 
CTGCTGTACA TCGGCTTTCG CGGCCTGGAC ACCATGCACT GCGCACCCAC CCCTGACTCA 
GGGGTCGTGT GCGGCCGCGT GGCCTGCTCC CCAACCCATG ACGAGCACCT GCTGCACGCC 
GGCCAGCCCT CTGAGGGGGA CCCGGACCTA GCCAAGATCC TGGAGGAGGT GCGCTACATT 
GCCCACCGCT TCCGCTGCCA GGATGAGAGT GAGGCCGTGT GCAGTGAGTG GAAGTTCGCG 
GCCTGTGTGG TGGACCGCCT GTGTCTCATG GCCTTCTCCG TCTTCACCAT CCTCTGCACC 
ATTGGCATCC TGATGTCGGC GCCCAACTTC GTTGAAGCTG TGTCCAAAGA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776940.1
CDS	43..1542
Translation	MRGSLCLALAASILHVSLQGEFQRKLYKDLVKNYNPLERPVANDSLPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMTWTDHYLQWNASEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQQCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGVLGKRSEKFYECCKEPYPDVTFTVSIRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVVLLNWCAWFLRMKRPGEDKVRPACQHNERRCSLASVEMSAVAGPPATNGNLLYIGFRGLDTMHCAPTPDSGVVCGRVACSPTHDEHLLHAGQPSEGDPDLAKILEEVRYIAHRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTILCTIGILMSAPNFVEAVSKDFA

Target ORF information:

RefSeq Version	NM_174515.2
Organism	Bos taurus(cattle)
Definition	Bos taurus cholinergic receptor, nicotinic, alpha 7 (CHRNA7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174515.2

ORF Insert Sequence:

ATGCGCGGCT CGTTGTGCCT GGCGCTGGCA GCGTCGATCT TGCACGTGTC CCTGCAAGGC 
GAGTTCCAGA GGAAGCTCTA CAAAGACCTG GTGAAGAACT ACAACCCCTT GGAGAGGCCG 
GTGGCCAACG ACTCGCTGCC GCTCACAGTC TACTTCTCCC TGAGCCTCCT GCAGATCATG 
GACGTGGATG AGAAGAACCA GGTGTTGACC ACCAACATTT GGCTACAAAT GACTTGGACA 
GATCACTATT TACAGTGGAA TGCATCCGAG TATCCAGGCG TGAAGACGGT TCGTTTCCCA 
GACGGCCAGA TCTGGAAGCC AGACATTCTC CTCTACAACA GTGCTGATGA ACGGTTTGAT 
GCTACGTTCC ACACCAATGT GTTAGTGAAT TCTTCTGGGC ACTGCCAGTA CCTCCCTCCA 
GGCATCTTTA AGAGCTCCTG CTACATCGAT GTGCGCTGGT TCCCATTTGA TGTGCAGCAG 
TGCAAACTGA AGTTTGGGTC CTGGTCTTAT GGAGGATGGT CCTTGGATCT GCAAATGCAG 
GAGGCAGACA TCAGCGGCTA TATCCCAAAT GGAGAATGGG ACCTTGTGGG GGTCTTGGGC 
AAGCGGAGTG AGAAGTTCTA CGAGTGCTGC AAAGAACCCT ACCCAGACGT GACCTTCACG 
GTCAGCATCC GCCGCAGGAC TCTCTACTAC GGCCTCAACC TGCTCATCCC CTGTGTGCTC 
ATCTCTGCCC TAGCCCTCCT CGTCTTCTTG CTCCCTGCAG ACTCTGGAGA GAAAATCTCC 
CTGGGAATCA CAGTTTTACT CTCCCTCACC GTCTTCATGC TGCTGGTAGC TGAGATCATG 
CCTGCGACCT CTGACTCAGT GCCCTTAATA GCCCAGTACT TCGCCAGCAC CATGATCATC 
GTGGGCCTCT CTGTGGTTGT CACGGTGATC GTGCTACAGT ACCACCACCA CGACCCTGAT 
GGTGGCAAGA TGCCCAAGTG GACCAGAGTC GTCCTCCTGA ACTGGTGTGC CTGGTTCCTT 
CGCATGAAGA GGCCGGGGGA GGACAAGGTG CGGCCAGCCT GCCAGCACAA CGAGCGGCGC 
TGCAGCCTGG CCAGCGTGGA GATGAGCGCA GTGGCGGGGC CGCCGGCCAC CAATGGCAAC 
CTGCTGTACA TCGGCTTTCG CGGCCTGGAC ACCATGCACT GCGCACCCAC CCCTGACTCA 
GGGGTCGTGT GCGGCCGCGT GGCCTGCTCC CCAACCCATG ACGAGCACCT GCTGCACGCC 
GGCCAGCCCT CTGAGGGGGA CCCGGACCTA GCCAAGATCC TGGAGGAGGT GCGCTACATT 
GCCCACCGCT TCCGCTGCCA GGATGAGAGT GAGGCCGTGT GCAGTGAGTG GAAGTTCGCG 
GCCTGTGTGG TGGACCGCCT GTGTCTCATG GCCTTCTCCG TCTTCACCAT CCTCTGCACC 
ATTGGCATCC TGATGTCGGC GCCCAACTTC GTTGAAGCTG TGTCCAAAGA CTTCGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substitutions of amino acids in the pore domain of homomeric ?7 nicotinic receptors for analogous residues present in heteromeric receptors modify gating, rectification and binding properties.
Journal of neurochemistry119(1)40-9(2011 Oct)
Criado M,Svobodov? L,Mulet J,Sala F,Sala S

A small cytoplasmic region adjacent to the fourth transmembrane segment of the ?7 nicotinic receptor is essential for its biogenesis.
FEBS letters585(15)2477-80(2011 Aug)
Criado M,Mulet J,Gerber S,Sala S,Sala F

An extracellular RRR motif flanking the M1 transmembrane domain governs the biogenesis of homomeric neuronal nicotinic acetylcholine receptors.
FEBS letters585(8)1169-74(2011 Apr)
Alves DS,Castello-Banyuls J,Faura CC,Ballesta JJ

Evidence of alpha 7 nicotinic acetylcholine receptor expression in retinal pigment epithelial cells.
Visual neuroscience27(5-6)139-47(2010 Nov)
Maneu V,Gerona G,Fern?ndez L,Cuenca N,Lax P

Role of loop 9 on the function of neuronal nicotinic receptors.
Biochimica et biophysica acta1798(3)654-9(2010 Mar)
Criado M,Castillo M,Mulet J,Sala F,Sala S

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CHRNA7 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報