nAChRalpha5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha5
Entrez Gene ID	34826
Full Name	nicotinic Acetylcholine Receptor alpha5
Synonyms	BG:DS05899.4,BG:DS05899.5,CG16878,CG32975,CG4498,CT14633,Dalpha5,Dmel\CG32975,Nic 34E,anon-WO02059370.44,da5,daf,lincRNA.128,lincRNA.S1055,nAChR,nAChRalpha-34E,nAcRalpha-34E,nAcRalpha-34e
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha5 Names CG32975 gene product from transcript CG32975-RD CG32975-PB CG32975-PD CG32975-PH Dalpha5 nAChRalpha5-PB nAChRalpha5-PD nAChRalpha5-PH nicotinic acetylcholine Receptor_34E nicotinic acetylcholine receptor alpha 34E nicotinic acetylcholine receptor alpha5
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34F2-34F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_205986.4	NP_995708.1	nicotinic acetylcholine receptor alpha5, isoform B
NM_001370005.1	NP_001356885.1	nicotinic acetylcholine receptor alpha5, isoform I
NM_205985.3	NP_995707.2	nicotinic acetylcholine receptor alpha5, isoform D
NM_001259098.3	NP_001246027.1	nicotinic acetylcholine receptor alpha5, isoform H

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chrna7	NP_031416.3
Rattus norvegicus (Norway rat)	Chrna7	NP_036964.3
Drosophila melanogaster (fruit fly)	nAcRalpha-34E	NP_001246027.1
Caenorhabditis elegans (roundworm)	acr-16	NP_505207.1
Caenorhabditis elegans (roundworm)	acr-25	NP_001023570.2
Caenorhabditis elegans (roundworm)	acr-7	NP_495647.1
Pan troglodytes (chimpanzee)	CHRNA7	XP_001164138.1
	CHRNA7	NP_001028055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008588	XP_314691.2
Caenorhabditis elegans (roundworm)	acr-11	NP_491906.1
Caenorhabditis elegans (roundworm)	acr-9	NP_510285.2
Caenorhabditis elegans (roundworm)	acr-14	NP_495716.1
Homo sapiens (human)	CHRNA7	NP_000737.1
Bos taurus (cattle)	CHRNA7	NP_776940.1
Gallus gallus (chicken)	CHRNA7	NP_989512.2
Caenorhabditis elegans (roundworm)	acr-10	NP_508692.2
Canis lupus familiaris (dog)	CHRNA7	XP_545813.4
Danio rerio (zebrafish)	chrna7	NP_957513.1
Xenopus tropicalis (tropical clawed frog)	LOC100493611	XP_002937717.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

D alpha 5 is a component of an alpha-bungarotoxin binding nicotinic acetylcholine receptor from the central nervous system of Drosophila
Title: The Drosophila acetylcholine receptor subunit D alpha5 is part of an alpha-bungarotoxin binding acetylcholine receptor.

The following nAChRalpha5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14690	NM_001259098.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant H (nAChRalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14690	NM_205986.4 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant B (nAChRalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14690	NM_205985.3 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant D (nAChRalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa87752	NM_001370005.1 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant I (nAChRalpha5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha5 ( NM_205986.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995708.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant B (nAChRalpha5), mRNA.

Price & Availability↑

CloneID	OFa14690
Clone ID Related Accession (Same CDS sequence)	NM_205986.4 , NM_205985.3 , NM_001259098.3
Accession Version	NM_205986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205986.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995708.1
CDS	1793..4219
Translation	MKNAQLKLTEVDDDELWLAVRLAHCSSNISSSSSTRTTSSNKRHNQQLTTLQPRSLSTKHHSNIASEQHNSQQQEPASKDEDVANHGRSNDQQTHLQQLDSSNMLSPKTAAAATAAGDEATTQQPTNIRLCARKRQRLRRRRKRKPATPNETDIKKQQQLSMPPFKTRKSTDTYSTPAAITSCPTATYMQCRASDNEFSIPISRHDRVSTATFAWVLHVLQVLLVSLQQWQLHVQQRSVLLFRRIAASTIAFISYLGSFAAQLRSSSSSSSNSSNSGNSSSTQILNGLNKHSWIFLLIYLNLSAKVCLAGYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGGDISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIRIVFLCWLPWILRMSRPGRPLILEFPTTPCSDTSSERKHQILSDVELKERSSKSLLANVLDIDDDFRHNCRPMTPGGTLPHNPAFYRTVYGQGDDGSIGPIGSTRMPDAVTHHTCIKSSTEYELGLILKEIRFITDQLRKDDECNDIANDWKFAAMVVDRLCLIIFTMFTILATIAVLLSAPHIIVS

Target ORF information:

RefSeq Version	NM_205986.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant B (nAChRalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205986.4

ORF Insert Sequence:

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha5 ( NM_205985.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995707.2 Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant D (nAChRalpha5), mRNA.

Price & Availability↑

CloneID	OFa14690
Clone ID Related Accession (Same CDS sequence)	NM_205986.4 , NM_205985.3 , NM_001259098.3
Accession Version	NM_205985.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha5, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_205985.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995707.2
CDS	1620..4046
Translation	MKNAQLKLTEVDDDELWLAVRLAHCSSNISSSSSTRTTSSNKRHNQQLTTLQPRSLSTKHHSNIASEQHNSQQQEPASKDEDVANHGRSNDQQTHLQQLDSSNMLSPKTAAAATAAGDEATTQQPTNIRLCARKRQRLRRRRKRKPATPNETDIKKQQQLSMPPFKTRKSTDTYSTPAAITSCPTATYMQCRASDNEFSIPISRHDRVSTATFAWVLHVLQVLLVSLQQWQLHVQQRSVLLFRRIAASTIAFISYLGSFAAQLRSSSSSSSNSSNSGNSSSTQILNGLNKHSWIFLLIYLNLSAKVCLAGYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGGDISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIRIVFLCWLPWILRMSRPGRPLILEFPTTPCSDTSSERKHQILSDVELKERSSKSLLANVLDIDDDFRHNCRPMTPGGTLPHNPAFYRTVYGQGDDGSIGPIGSTRMPDAVTHHTCIKSSTEYELGLILKEIRFITDQLRKDDECNDIANDWKFAAMVVDRLCLIIFTMFTILATIAVLLSAPHIIVS

Target ORF information:

RefSeq Version	NM_205985.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant D (nAChRalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205985.3

ORF Insert Sequence:

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha5 ( NM_001259098.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001246027.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant H (nAChRalpha5), mRNA.

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CloneID	OFa14690
Clone ID Related Accession (Same CDS sequence)	NM_205986.4 , NM_205985.3 , NM_001259098.3
Accession Version	NM_001259098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha5, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001259098.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001246027.1
CDS	1864..4290
Translation	MKNAQLKLTEVDDDELWLAVRLAHCSSNISSSSSTRTTSSNKRHNQQLTTLQPRSLSTKHHSNIASEQHNSQQQEPASKDEDVANHGRSNDQQTHLQQLDSSNMLSPKTAAAATAAGDEATTQQPTNIRLCARKRQRLRRRRKRKPATPNETDIKKQQQLSMPPFKTRKSTDTYSTPAAITSCPTATYMQCRASDNEFSIPISRHDRVSTATFAWVLHVLQVLLVSLQQWQLHVQQRSVLLFRRIAASTIAFISYLGSFAAQLRSSSSSSSNSSNSGNSSSTQILNGLNKHSWIFLLIYLNLSAKVCLAGYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGGDISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIRIVFLCWLPWILRMSRPGRPLILEFPTTPCSDTSSERKHQILSDVELKERSSKSLLANVLDIDDDFRHNCRPMTPGGTLPHNPAFYRTVYGQGDDGSIGPIGSTRMPDAVTHHTCIKSSTEYELGLILKEIRFITDQLRKDDECNDIANDWKFAAMVVDRLCLIIFTMFTILATIAVLLSAPHIIVS

Target ORF information:

RefSeq Version	NM_001259098.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant H (nAChRalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001259098.3

ORF Insert Sequence:

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha5 ( NM_001370005.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001356885.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant I (nAChRalpha5), mRNA.

Price & Availability↑

CloneID	OFa87752
Clone ID Related Accession (Same CDS sequence)	NM_001370005.1
Accession Version	NM_001370005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha5, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAGCCA TATGGGCGAG GTGGTTATTG TTATTGGTTT 
TATTATAAAA TCAATTTGTT AATTATTAAA TTAATAACGA AACTCTTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356885.1
CDS	1864..4377
Translation	MKNAQLKLTEVDDDELWLAVRLAHCSSNISSSSSTRTTSSNKRHNQQLTTLQPRSLSTKHHSNIASEQHNSQQQEPASKDEDVANHGRSNDQQTHLQQLDSSNMLSPKTAAAATAAGDEATTQQPTNIRLCARKRQRLRRRRKRKPATPNETDIKKQQQLSMPPFKTRKSTDTYSTPAAITSCPTATYMQCRASDNEFSIPISRHDRVSTATFAWVLHVLQVLLVSLQQWQLHVQQRSVLLFRRIAASTIAFISYLGSFAAQLRSSSSSSSNSSNSGNSSSTQILNGLNKHSWIFLLIYLNLSAKVCLAGYHEKRLLHDLLDPYNTLERPVLNESDPLQLSFGLTLMQIIDVDEKNQLLVTNVWLKLEWNDMNLRWNTSDYGGVKDLRIPPHRIWKPDVLMYNSADEGFDGTYQTNVVVRNNGSCLYVPPGIFKSTCKIDITWFPFDDQRCEMKFGSWTYDGFQLDLQLQDETGGDISSYVLNGEWELLGVPGKRNEIYYNCCPEPYIDITFAIIIRRRTLYYFFNLIIPCVLIASMALLGFTLPPDSGEKLSLGVTILLSLTVFLNMVAETMPATSDAVPLLGTYFNCIMFMVASSVVSTILILNYHHRNADTHEMSEWIRIVFLCWLPWILRMSRPGRPLILEFPTTPCSDTSSERKHQILSDVELKERSSKSLLANVLDIDDDFRHNCRPMTPGGTLPHNPAFYRTVYGQGDDGSIGPIGSTRMPDAVTHHTCIKSSTEYELGLILKEIRFITDQLRKDDECNDIANDWKFAAMVVDRLCLIIFTMFTILATIAVLLSAPHIIVS*PYGRGGYCYWFYYKINLLIIKLITKLFK

Target ORF information:

RefSeq Version	NM_001370005.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha5, transcript variant I (nAChRalpha5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370005.1

ORF Insert Sequence:

ATGAAAAATG CACAACTGAA ACTGACTGAA GTTGACGATG ATGAGCTGTG GCTGGCAGTA 
AGATTAGCGC ACTGCAGCAG CAACATCAGC AGCAGTAGCA GCACAAGAAC CACCAGCAGC 
AACAAGAGGC ACAACCAGCA ACTCACAACA CTGCAACCAA GGAGCTTAAG TACAAAACAC 
CACAGCAACA TTGCAAGCGA GCAGCACAAT AGCCAGCAAC AGGAGCCAGC ATCGAAGGAC 
GAGGATGTAG CCAACCACGG TAGAAGCAAT GACCAGCAGA CGCATCTGCA ACAGCTAGAC 
AGCAGCAACA TGTTGTCGCC AAAGACAGCC GCAGCAGCAA CTGCTGCCGG CGATGAAGCA 
ACAACCCAAC AACCAACAAA CATAAGACTG TGTGCACGCA AGCGACAACG ATTGCGTCGC 
CGACGAAAAA GAAAACCAGC AACCCCAAAC GAAACAGATA TCAAGAAACA ACAGCAACTT 
AGCATGCCTC CCTTCAAAAC GCGCAAATCC ACGGACACCT ACAGCACACC AGCAGCAATA 
ACCAGCTGTC CGACAGCCAC CTACATGCAA TGTCGAGCCA GCGACAATGA GTTCAGTATT 
CCGATATCGA GACATGATAG AGTATCCACG GCCACATTCG CCTGGGTGTT GCATGTGCTG 
CAGGTGCTGC TCGTGTCGCT GCAACAGTGG CAACTTCACG TGCAACAGCG ATCGGTGCTA 
CTGTTCAGAA GGATCGCAGC GAGCACCATC GCCTTCATTT CCTATTTAGG CAGCTTTGCA 
GCGCAACTGA GGAGCAGCAG CAGTAGCAGC AGCAACAGCA GCAACAGCGG CAACAGCAGC 
AGCACGCAAA TATTAAACGG ACTTAATAAA CACTCATGGA TATTTTTATT GATATATTTG 
AATTTATCTG CTAAAGTTTG CCTAGCAGGA TATCATGAAA AGAGACTGTT ACACGATCTT 
TTGGATCCCT ATAATACACT AGAACGTCCC GTTCTCAATG AATCGGACCC GTTACAATTA 
AGCTTTGGTT TAACTTTAAT GCAAATTATC GATGTGGACG AGAAAAATCA ATTGCTAGTC 
ACTAATGTGT GGTTAAAACT GGAGTGGAAC GACATGAATC TCCGCTGGAA CACCTCCGAC 
TATGGCGGAG TTAAGGATCT GCGAATACCG CCGCATCGCA TCTGGAAGCC GGACGTGCTG 
ATGTACAACA GTGCGGATGA GGGATTTGAT GGCACCTACC AGACGAACGT GGTGGTGCGG 
AACAACGGCT CGTGTCTATA CGTTCCGCCG GGGATCTTCA AGTCGACGTG CAAGATCGAC 
ATCACGTGGT TCCCCTTCGA TGACCAGCGG TGCGAGATGA AGTTCGGCAG TTGGACCTAC 
GACGGATTCC AGCTGGATTT ACAATTACAA GATGAAACTG GCGGTGATAT CAGCAGTTAC 
GTGCTCAACG GCGAGTGGGA ACTACTGGGT GTGCCCGGCA AACGTAACGA AATCTATTAC 
AACTGCTGCC CGGAACCCTA TATAGACATC ACCTTCGCCA TCATCATCCG CCGACGAACA 
CTGTACTATT TCTTCAACCT GATCATACCT TGTGTACTGA TTGCCTCCAT GGCCTTGCTC 
GGATTCACTC TGCCGCCAGA TTCGGGTGAA AAATTATCAC TGGGTGTTAC CATCTTGCTC 
TCGCTGACCG TGTTTCTGAA TATGGTTGCC GAGACAATGC CGGCTACTTC CGATGCGGTG 
CCATTGCTGG GTACATATTT CAATTGCATA ATGTTTATGG TAGCTTCATC CGTTGTGTCA 
ACGATTTTAA TATTAAATTA TCATCATCGA AATGCTGATA CGCACGAAAT GTCCGAATGG 
ATACGCATCG TGTTTTTGTG CTGGCTGCCA TGGATATTGC GAATGAGTCG ACCAGGACGA 
CCGCTGATCC TGGAGTTCCC GACCACGCCC TGTTCGGACA CATCCTCCGA GCGGAAGCAC 
CAGATACTCT CCGACGTTGA GCTGAAAGAG CGCTCGTCGA AATCGCTGCT GGCCAACGTA 
CTAGACATCG ATGATGACTT CCGGCACAAT TGTCGCCCCA TGACGCCCGG CGGAACACTG 
CCACACAACC CGGCTTTCTA TCGCACGGTT TATGGACAAG GCGACGATGG CAGCATTGGG 
CCAATTGGCA GCACCCGAAT GCCGGATGCG GTCACCCATC ATACGTGCAT CAAATCATCA 
ACTGAATATG AATTAGGTTT AATCTTAAAG GAAATTCGCT TTATAACTGA TCAGCTACGT 
AAAGATGACG AGTGCAATGA CATTGCCAAT GATTGGAAAT TTGCAGCTAT GGTCGTTGAC 
AGACTGTGCC TTATCATATT CACAATGTTC ACAATATTAG CCACAATAGC TGTACTACTA 
TCAGCACCAC ATATTATTGT CTCGTAGCCA TATGGGCGAG GTGGTTATTG TTATTGGTTT 
TATTATAAAA TCAATTTGTT AATTATTAAA TTAATAACGA AACTCTTTAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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