NPHP4 cDNA ORF clone, Canis lupus familiaris(dog)

The following NPHP4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHP4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287811 XM_022418039.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287812 XM_022418040.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287813 XM_022418041.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287814 XM_022418042.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287815 XM_022418043.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287816 XM_022418044.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287817 XM_022418045.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287818 XM_022418046.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe287819 XM_022418047.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe287820 XM_022418048.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe36603 XM_014113843.2
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36603 XM_014113844.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36604 XM_014113845.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36605 XM_014113846.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36606 XM_014113847.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36607 XM_014113850.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36607 XM_014113848.2
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36608 XM_014113849.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36609 XM_014113851.1
Latest version!
Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe06756 NM_001135788.1
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe06756 XM_005620413.2
Latest version!
Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe36607 XM_014113848.1 Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe36603 XM_014113843.1 Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe06756 XM_005620413.1 Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287811
    Clone ID Related Accession (Same CDS sequence) XM_022418039.1
    Accession Version XM_022418039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGCAGGTGCT
    CCCGTGGGAA GACCTGGTGA GGAGCCCCCA GTCTATGTCC GATGTAGCGA CCCCAACGTC
    ATCTGCGAGA CCCAGAATGT GGGCCCCAGG GAGCCTCGGG ACGTGTTCCT GAAGGTGGCC
    AGCGGTCCAA GCCCAGAGAG CAGAGACTTC TTTGTCGCCG TTTACGCGGA TCGTTGGCTG
    GCGACACCCA TCCAAATATG GCAGGTCTAC CTCCACTCCT TGCAGCGTGT GGACGTCTCC
    TGTGTCACAG GCCAGCGGAC ACGCCTATCC CTTGTCCTTC GAGGGACACG GACGGTTAGG
    AGAGTGCAGG CTTTCACCTC ACACCCTCAG GAGCTGAAGA CTGACCCCAA AGGTGTGTTC
    GTGCTGCCAC CACATGGGGT GCAGGACCTG CATGTCGGTG TGAGGCCCCG CAGGGCAGGG
    AGCCGGTTCA TCCACCTCAA CCTGGTCGAC GTGGACTACC ACCAGCTGGT GGCCTCATGG
    CTCGTGTGCC TCTCCTGCCG CCCGCCTCTC ATCTCCAAGG CTTTCGAGAT CACACTGGCT
    GCAGGGAAAG GCAGAGGCGC CAACAAGCGG ATCACCTACA CCAACCCTTA CCCCTCCCAG
    AGGACCTATC ACCTGTACAG CGACCACCCT GACCTGCTGC AGTTCAGAGA GGACTCCTTC
    CAGATTGGGG GAGGAGAGAC CTACACCATC GGCCTGTGGT TCGCACCTCG GCAGAGCCCC
    GGGGAGGAGG AGATTCTGAT TTACATCAAT GACCACGAAG ACAAAAATGA AGAAGCCTTC
    TGCGTCAAGG TCGTGTACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273747.1
    CDS288..4751
    Translation

    Target ORF information:

    RefSeq Version XM_022418039.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGCAGGTGCT
    CCCGTGGGAA GACCTGGTGA GGAGCCCCCA GTCTATGTCC GATGTAGCGA CCCCAACGTC
    ATCTGCGAGA CCCAGAATGT GGGCCCCAGG GAGCCTCGGG ACGTGTTCCT GAAGGTGGCC
    AGCGGTCCAA GCCCAGAGAG CAGAGACTTC TTTGTCGCCG TTTACGCGGA TCGTTGGCTG
    GCGACACCCA TCCAAATATG GCAGGTCTAC CTCCACTCCT TGCAGCGTGT GGACGTCTCC
    TGTGTCACAG GCCAGCGGAC ACGCCTATCC CTTGTCCTTC GAGGGACACG GACGGTTAGG
    AGAGTGCAGG CTTTCACCTC ACACCCTCAG GAGCTGAAGA CTGACCCCAA AGGTGTGTTC
    GTGCTGCCAC CACATGGGGT GCAGGACCTG CATGTCGGTG TGAGGCCCCG CAGGGCAGGG
    AGCCGGTTCA TCCACCTCAA CCTGGTCGAC GTGGACTACC ACCAGCTGGT GGCCTCATGG
    CTCGTGTGCC TCTCCTGCCG CCCGCCTCTC ATCTCCAAGG CTTTCGAGAT CACACTGGCT
    GCAGGGAAAG GCAGAGGCGC CAACAAGCGG ATCACCTACA CCAACCCTTA CCCCTCCCAG
    AGGACCTATC ACCTGTACAG CGACCACCCT GACCTGCTGC AGTTCAGAGA GGACTCCTTC
    CAGATTGGGG GAGGAGAGAC CTACACCATC GGCCTGTGGT TCGCACCTCG GCAGAGCCCC
    GGGGAGGAGG AGATTCTGAT TTACATCAAT GACCACGAAG ACAAAAATGA AGAAGCCTTC
    TGCGTCAAGG TCGTGTACCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287812
    Clone ID Related Accession (Same CDS sequence) XM_022418040.1
    Accession Version XM_022418040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273748.1
    CDS287..4747
    Translation

    Target ORF information:

    RefSeq Version XM_022418040.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287813
    Clone ID Related Accession (Same CDS sequence) XM_022418041.1
    Accession Version XM_022418041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273749.1
    CDS288..4748
    Translation

    Target ORF information:

    RefSeq Version XM_022418041.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287814
    Clone ID Related Accession (Same CDS sequence) XM_022418042.1
    Accession Version XM_022418042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4461bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273750.1
    CDS289..4749
    Translation

    Target ORF information:

    RefSeq Version XM_022418042.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287815
    Clone ID Related Accession (Same CDS sequence) XM_022418043.1
    Accession Version XM_022418043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273751.1
    CDS288..4745
    Translation

    Target ORF information:

    RefSeq Version XM_022418043.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287816
    Clone ID Related Accession (Same CDS sequence) XM_022418044.1
    Accession Version XM_022418044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273752.1
    CDS289..4746
    Translation

    Target ORF information:

    RefSeq Version XM_022418044.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287817
    Clone ID Related Accession (Same CDS sequence) XM_022418045.1
    Accession Version XM_022418045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GCAGGTGCTC CCGTGGGAAG ACCTGGTGAG GAGCCCCCAG TCTATGTCCG ATGTAGCGAC
    CCCAACGTCA TCTGCGAGAC CCAGAATGTG GGCCCCAGGG AGCCTCGGGA CGTGTTCCTG
    AAGGTGGCCA GCGGTCCAAG CCCAGAGAGC AGAGACTTCT TTGTCGCCGT TTACGCGGAT
    CGTTGGCTGG CGACACCCAT CCAAATATGG CAGGTCTACC TCCACTCCTT GCAGCGTGTG
    GACGTCTCCT GTGTCACAGG CCAGCGGACA CGCCTATCCC TTGTCCTTCG AGGGACACGG
    ACGGTTAGGA GAGTGCAGGC TTTCACCTCA CACCCTCAGG AGCTGAAGAC TGACCCCAAA
    GGTGTGTTCG TGCTGCCACC ACATGGGGTG CAGGACCTGC ATGTCGGTGT GAGGCCCCGC
    AGGGCAGGGA GCCGGTTCAT CCACCTCAAC CTGGTCGACG TGGACTACCA CCAGCTGGTG
    GCCTCATGGC TCGTGTGCCT CTCCTGCCGC CCGCCTCTCA TCTCCAAGGC TTTCGAGATC
    ACACTGGCTG CAGGGAAAGG CAGAGGCGCC AACAAGCGGA TCACCTACAC CAACCCTTAC
    CCCTCCCAGA GGACCTATCA CCTGTACAGC GACCACCCTG ACCTGCTGCA GTTCAGAGAG
    GACTCCTTCC AGATTGGGGG AGGAGAGACC TACACCATCG GCCTGTGGTT CGCACCTCGG
    CAGAGCCCCG GGGAGGAGGA GATTCTGATT TACATCAATG ACCACGAAGA CAAAAATGAA
    GAAGCCTTCT GCGTCAAGGT CGTGTACCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273753.1
    CDS288..4640
    Translation

    Target ORF information:

    RefSeq Version XM_022418045.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GCAGGTGCTC CCGTGGGAAG ACCTGGTGAG GAGCCCCCAG TCTATGTCCG ATGTAGCGAC
    CCCAACGTCA TCTGCGAGAC CCAGAATGTG GGCCCCAGGG AGCCTCGGGA CGTGTTCCTG
    AAGGTGGCCA GCGGTCCAAG CCCAGAGAGC AGAGACTTCT TTGTCGCCGT TTACGCGGAT
    CGTTGGCTGG CGACACCCAT CCAAATATGG CAGGTCTACC TCCACTCCTT GCAGCGTGTG
    GACGTCTCCT GTGTCACAGG CCAGCGGACA CGCCTATCCC TTGTCCTTCG AGGGACACGG
    ACGGTTAGGA GAGTGCAGGC TTTCACCTCA CACCCTCAGG AGCTGAAGAC TGACCCCAAA
    GGTGTGTTCG TGCTGCCACC ACATGGGGTG CAGGACCTGC ATGTCGGTGT GAGGCCCCGC
    AGGGCAGGGA GCCGGTTCAT CCACCTCAAC CTGGTCGACG TGGACTACCA CCAGCTGGTG
    GCCTCATGGC TCGTGTGCCT CTCCTGCCGC CCGCCTCTCA TCTCCAAGGC TTTCGAGATC
    ACACTGGCTG CAGGGAAAGG CAGAGGCGCC AACAAGCGGA TCACCTACAC CAACCCTTAC
    CCCTCCCAGA GGACCTATCA CCTGTACAGC GACCACCCTG ACCTGCTGCA GTTCAGAGAG
    GACTCCTTCC AGATTGGGGG AGGAGAGACC TACACCATCG GCCTGTGGTT CGCACCTCGG
    CAGAGCCCCG GGGAGGAGGA GATTCTGATT TACATCAATG ACCACGAAGA CAAAAATGAA
    GAAGCCTTCT GCGTCAAGGT CGTGTACCAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287818
    Clone ID Related Accession (Same CDS sequence) XM_022418046.1
    Accession Version XM_022418046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGTGC AGCAAGTGGA GTCAGGGACT
    GTCCAGTTCC ACTTCTCGCT GAGCCCTGAG GGACACCTGG ACACACCCAC GGGGCTGGTC
    CACAGCCCTG AGGCGGAGCG GCAGCCCTCC AGGAAGCCAC CCACATCTCC TGCAAACCTG
    CCAGCACTGC CGCCCCAGGG GCTCGCTGCC CTCCAGGACT CGCCGGTGGG ACCAGGGTTG
    TCCCTTTCCC AGCTCGCAGC TTCCCCACAG CCTACCACCC GCTCCCGCTC AGCCACACCC
    CCTCCACGGC AACCCGACAG CTCTAAGCCC CCGCCGGCCC AGGAGCAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273754.1
    CDS288..4568
    Translation

    Target ORF information:

    RefSeq Version XM_022418046.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGTGC AGCAAGTGGA GTCAGGGACT
    GTCCAGTTCC ACTTCTCGCT GAGCCCTGAG GGACACCTGG ACACACCCAC GGGGCTGGTC
    CACAGCCCTG AGGCGGAGCG GCAGCCCTCC AGGAAGCCAC CCACATCTCC TGCAAACCTG
    CCAGCACTGC CGCCCCAGGG GCTCGCTGCC CTCCAGGACT CGCCGGTGGG ACCAGGGTTG
    TCCCTTTCCC AGCTCGCAGC TTCCCCACAG CCTACCACCC GCTCCCGCTC AGCCACACCC
    CCTCCACGGC AACCCGACAG CTCTAAGCCC CCGCCGGCCC AGGAGCAGGA GCCCCCCTCG
    GAGGGCAGGA TCTCTCACCT GGAGGCCCAG CTGAGCCAGG CGTCCTTGGT CCTGGGAGCC
    TCTGTTGCTG AACAGCTGCA GGAGCTGCCT TTTACGCCGG TGCACACCCC GATTGTTGTG
    GGAGCCCAGA CCAGGAGCTC TGGAAGTAAG CTCTCACGAG CCTCAATGGT GCTCCTGCAA
    GCCTGCGGCT TTCCTGAGAT TCTGGATGCC AATAAGCAAC CAGCCGAGGC TGTAAATCCT
    ACAGATCCCG TGAAATTCAA CCCCCAAAAG GAAGAATCAG ATTGTCTACA AAGCAATGAG
    ATAGTGCTGC ATTTTCTTGC CTTTAGCAGA GTGTCCCAGG ACTGCCAGGG AGCACCGTGG
    CCAAAGACCG TGTATTTCAC CTTCCAGTTC TATCGCTTCC CACCCGTGAC AACGCCACGC
    TTGCAGCTGA TCAGGCTGGA CGAGACGGGC AGGACAGGCT CTGGTTCCCT GTTGTATCTC
    CTGGTTCTCA CTGACAAAGA TGGCTCCTCT GATGCTGGGA CACCTGGCTT TCAACTCAAG
    TACCTGGTGG AACCTGGGTT CCTGAAACCC GGAGAACAGC GCTGGTTCAC CCACTACATG
    GCTGCTCAGA CCCTCCAGAT CGACATCTGG GATGGAGACT CCTTGCTGCT CGTGGGATCT
    GCGGCCGTCC AGATGAAGCA TCTCCTCCGC CAAGGCCGGC CGGCTGTGCA GGTCCTGCAT
    GAGCTTGAGG TCGTGGCGAC TGAATATGAA CAGGATGTGG TGGTGAGTGG AGATGTGACC
    AGATTTGGCT GTGTCAAGCC CATCGGTGTC TACACAGTGG TAAAGGGCTG GCTGCACCTG
    ACGTTGGCCA ACGTGGGTCA TGTGTGTGAG CAGAGAGTGA GAGATTCCAG CTCATTGCCA
    CCTTCCAGGT CACGGGTCAT TTCGAATGAT GGAGCCAGAC ACTTCGAGGG AGGCAGTCTC
    ATCTCGCGCG GGGGTCCGAG ATCAGTAAAA AATGTTGTCC AAGCACAGAA GCTGGCTGAC
    GTGGACCGTG AGCTGGCCGC CATGCTGTTC ACCCACATGC AACATGGGAG CAAGGGGCCC
    CAAGGTGCTT GCCGTGAGGG GGATGCCGTC CGCAGGCGCA AGCTGGAGCG GATGAGGTCC
    GTGCGCCTTC AAGAGTCCAG AGGAGAGCTC AGCTGCTGGG GGGCCAGCAT GTTGATTCAG
    CAGAATATCC GCGCACAGCA CGCACGTGAC CTGCAGGTCA TTGCTGCCTA TCGGGAGCGT
    ACGAAGGCAG AGAGCATCAC CAGTGCGCTG AGCCTGGCCA TCACCACGAT GCACACAATC
    CACGTCACAC TGGGCACTGC CGAGTTCTTC GAGTTTGCCC TTAGGAACCC CCACAACACA
    CAGCACACAG TGACCATTGA GGTGGACAAT CCTGAGCTCA GCATCATCCA GGACAGTCAG
    GAGTGGAGAT ACTTCAAAGA CGCGACCAAG CTGCACACGC CGGTGGAGGA GGACATGTTC
    CATCTGCGGG GCAGCCTTGC CCCGCAGATC TTCCTGCGCC CACGAGAGAC CGCTCACATC
    CCCTTCAAAT ACCAGACCTT CTCTGTAGGG CAACTGGCCC TGGCGCAGCC CTCTGCTGAG
    CTCGGATCTG AGAAGGACAC GGATACCGGA TTGCCCCAGA AATCCAGTGC AATGCCCACT
    AAGCATGCCA AGGTCTTGTT CCGGGGGAGT GGCGGTAAGC CCATCGCCGT GCTTTGCCTA
    ACCGTGGAGA CCCAGCCCCA TGTGGTGGAC CAGGTTTTCC GCTTCTACCA CCCAGAGCTC
    ACCTTCCTGA AGAAGGCCAT CCGCCTGCCT CCCTGGCACA CGCTTCCAGC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGGG CCCCAGGGAG CCTCGGGACG TGTTCCTGAA GGTGGCCAGC
    GGTCCAAGCC CAGAGAGCAG AGACTTCTTT GTCGCCGTTT ACGCGGATCG TTGGCTGGCG
    ACACCCATCC AAATATGGCA GGTCTACCTC CACTCCTTGC AGCGTGTGGA CGTCTCCTGT
    GTCACAGGCC AGCGGACACG CCTATCCCTT GTCCTTCGAG GGACACGGAC GGTTAGGAGA
    GTGCAGGCTT TCACCTCACA CCCTCAGGAG CTGAAGACTG ACCCCAAAGG TGTGTTCGTG
    CTGCCACCAC ATGGGGTGCA GGACCTGCAT GTCGGTGTGA GGCCCCGCAG GGCAGGGAGC
    CGGTTCATCC ACCTCAACCT GGTCGACGTG GACTACCACC AGCTGGTGGC CTCATGGCTC
    GTGTGCCTCT CCTGCCGCCC GCCTCTCATC TCCAAGGCTT TCGAGATCAC ACTGGCTGCA
    GGGAAAGGCA GAGGCGCCAA CAAGCGGATC ACCTACACCA ACCCTTACCC CTCCCAGAGG
    ACCTATCACC TGTACAGCGA CCACCCTGAC CTGCTGCAGT TCAGAGAGGA CTCCTTCCAG
    ATTGGGGGAG GAGAGACCTA CACCATCGGC CTGTGGTTCG CACCTCGGCA GAGCCCCGGG
    GAGGAGGAGA TTCTGATTTA CATCAATGAC CACGAAGACA AAAATGAAGA AGCCTTCTGC
    GTCAAGGTCG TGTACCAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287819
    Clone ID Related Accession (Same CDS sequence) XM_022418047.1
    Accession Version XM_022418047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGCAGGTGCT
    CCCGTGGGAA GACCTGGTGA GGAGCCCCCA GTCTATGTCC GATGTAGCGA CCCCAACGTC
    ATCTGCGAGA CCCAGAATGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273755.1
    CDS290..4033
    Translation

    Target ORF information:

    RefSeq Version XM_022418047.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGCAGGTGCT
    CCCGTGGGAA GACCTGGTGA GGAGCCCCCA GTCTATGTCC GATGTAGCGA CCCCAACGTC
    ATCTGCGAGA CCCAGAATGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287820
    Clone ID Related Accession (Same CDS sequence) XM_022418048.1
    Accession Version XM_022418048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273756.1
    CDS290..4030
    Translation

    Target ORF information:

    RefSeq Version XM_022418048.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGTCAGCCG AAAAGAGGCT AGAGAAGGAA GCTGCTCCCA CGGTGGTTCC GGACAGCTTC 
    ATACTGATGA CCTCTCTTTC GAGGGCTGCA GAAGAGTATT CCCTTCCCTC AGCAGAGCCA
    GCAGAAACTG TACTCCTAGA GGCCGAAGTG ACCCTCGGGA CTGGCAGAAT GAATGACTGG
    CTCAGGATTT TTGACCAAAA CGTGCTGGTC CCTCCCCACC CACAGAGAGC ACGCCAGCTT
    TTAAAGGAAT CAACAGCATT TCAGTGTGTC CTGAAGTGGC TTGACGGGCC ACTAATTAAG
    CAGGGAGTGC TGGAGGTGCT TTCGGAGGTG GGCTGCCATC TGCGTGTGTC TCTCTTTGAT
    GTCACCTACC GACACTTTTT CGGGAGGACA TGGAAGACCG CGGTGAGGCC GGCTGAGCAG
    CTTTCCAGGC AGCCGTCTAG GATCATCTTT GATGAGCCCT TGTACTTTCA CACGTCCTTG
    AACCACCCTA ACGTGGTGGC TGTGGTTGAG GTGGTCACCG AAGGCCGGAA GCAGGATGGC
    TCTCTCCAGG CTCTGTCATG TGGCTTTGGA ATTCTTCGGA TCTTTGGCAA CAAACTGGAA
    TCTCCTACCT CTGCCTCCCA GGACAAGCGG TTGAGGCTGT ATCACGGCAC TCCCCGAGCC
    CTCCTGCACC CACTGCTCCA GGACCCTTTC GAACAAAATA AACACATGAC CCTCATTGAG
    AGCTGCAGCC TGCAATACAC TCTGAAGCCA CACCCGCTCC TGGAGCCCGT GTTCCATCTT
    CTTCCCCAGA ACCTTCTGGT GTCTGGTCTG CAGCACATCC CTGGCCTCGT TCCAGCTCAT
    GGAGAAAAGG GGGACACCCT CCGAAAGCCT CGCCTTCAGA AGTCTGTCAC ATGGTACTTG
    GATGATTTAT TCTTCACGCT ATATCCTTCC CTTGAGAAGT TTGAGGAAGA GCTTCTGGAA
    CTCCTCATCA GTGATAACTT CCGGGAGGGG GACAGTCCAC TAGATGGCAG CACGCTGGAG
    ATCTTGGAGC GCCGCCTGTG TGTGGGCGTG CACAATGGTC TGGGCTTCGT GCAGAGGCCA
    CACGTGGTGG TGCTGGTGCC CGAGATGGAT GTGGCCTTGA CGCGCTCTGC CAGTTTCAGC
    AGGAAAGTTG GCTCCTCTTC GAAGACCAGC TCTGGAAACC AGGCTCTAGT TTTGAGAAGC
    CGCCTCCGAC TCCCTGAGAT GGTCCATCAC CCTGCGTTTG CCATAGTCTT TCAACTGGAG
    TACGTGTTCA CCAGCCCTTC TGGAGTGGAC AGCAAGGCAG CCTCGGTCAC CTCTTTGTCC
    AGTGTGGCGT GTATGCACAT AGTTCGCTGG GCTGTTTGGA ACCCCTTGCT GGAATCCGGC
    TCTGGGAAGG TGATCTTACC TCTGCAGGGT GGGATCCGGC CCAACCCCTC ACACTGTTTG
    GTCTATAAAG TAACCCCTGC CAGCATGAGC TCTGAAGAGG TGCAGCAAGT GGAGTCAGGG
    ACTGTCCAGT TCCACTTCTC GCTGAGCCCT GAGGGACACC TGGACACACC CACGGGGCTG
    GTCCACAGCC CTGAGGCGGA GCGGCAGCCC TCCAGGAAGC CACCCACATC TCCTGCAAAC
    CTGCCAGCAC TGCCGCCCCA GGGGCTCGCT GCCCTCCAGG ACTCGCCGGT GGGACCAGGG
    TTGTCCCTTT CCCAGCTCGC AGCTTCCCCA CAGCCTACCA CCCGCTCCCG CTCAGCCACA
    CCCCCTCCAC GGCAACCCGA CAGCTCTAAG CCCCCGCCGG CCCAGGAGCA GGAGCCCCCC
    TCGGAGGGCA GGATCTCTCA CCTGGAGGCC CAGCTGAGCC AGGCGTCCTT GGTCCTGGGA
    GCCTCTGTTG CTGAACAGCT GCAGGAGCTG CCTTTTACGC CGGTGCACAC CCCGATTGTT
    GTGGGAGCCC AGACCAGGAG CTCTGGAAGT AAGCTCTCAC GAGCCTCAAT GGTGCTCCTG
    CAAGCCTGCG GCTTTCCTGA GATTCTGGAT GCCAATAAGC AACCAGCCGA GGCTGTAAAT
    CCTACAGATC CCGTGAAATT CAACCCCCAA AAGGAAGAAT CAGATTGTCT ACAAAGCAAT
    GAGATAGTGC TGCATTTTCT TGCCTTTAGC AGAGTGTCCC AGGACTGCCA GGGAGCACCG
    TGGCCAAAGA CCGTGTATTT CACCTTCCAG TTCTATCGCT TCCCACCCGT GACAACGCCA
    CGCTTGCAGC TGATCAGGCT GGACGAGACG GGCAGGACAG GCTCTGGTTC CCTGTTGTAT
    CTCCTGGTTC TCACTGACAA AGATGGCTCC TCTGATGCTG GGACACCTGG CTTTCAACTC
    AAGTACCTGG TGGAACCTGG GTTCCTGAAA CCCGGAGAAC AGCGCTGGTT CACCCACTAC
    ATGGCTGCTC AGACCCTCCA GATCGACATC TGGGATGGAG ACTCCTTGCT GCTCGTGGGA
    TCTGCGGCCG TCCAGATGAA GCATCTCCTC CGCCAAGGCC GGCCGGCTGT GCAGGTCCTG
    CATGAGCTTG AGGTCGTGGC GACTGAATAT GAACAGGATG TGGTGGTGAG TGGAGATGTG
    ACCAGATTTG GCTGTGTCAA GCCCATCGGT GTCTACACAG TGGTAAAGGG CTGGCTGCAC
    CTGACGTTGG CCAACGTGGG TCATGTGTGT GAGCAGAGAG TGAGAGATTC CAGCTCATTG
    CCACCTTCCA GGTCACGGGT CATTTCGAAT GATGGAGCCA GACACTTCGA GGGAGGCAGT
    CTCATCTCGC GCGGGGGTCC GAGATCAGTA AAAAATGTTG TCCAAGCACA GAAGCTGGCT
    GACGTGGACC GTGAGCTGGC CGCCATGCTG TTCACCCACA TGCAACATGG GAGCAAGGGG
    CCCCAAGGTG CTTGCCGTGA GGGGGATGCC GTCCGCAGGC GCAAGCTGGA GCGGATGAGG
    TCCGTGCGCC TTCAAGAGTC CAGAGGAGAG CTCAGCTGCT GGGGGGCCAG CATGTTGATT
    CAGCAGAATA TCCGCGCACA GCACGCACGT GACCTGCAGG TCATTGCTGC CTATCGGGAG
    CGTACGAAGG CAGAGAGCAT CACCAGTGCG CTGAGCCTGG CCATCACCAC GATGCACACA
    ATCCACGTCA CACTGGGCAC TGCCGAGTTC TTCGAGTTTG CCCTTAGGAA CCCCCACAAC
    ACACAGCACA CAGTGACCAT TGAGGTGGAC AATCCTGAGC TCAGCATCAT CCAGGACAGT
    CAGGAGTGGA GATACTTCAA AGACGCGACC AAGCTGCACA CGCCGGTGGA GGAGGACATG
    TTCCATCTGC GGGGCAGCCT TGCCCCGCAG ATCTTCCTGC GCCCACGAGA GACCGCTCAC
    ATCCCCTTCA AATACCAGAC CTTCTCTGTA GGGCAACTGG CCCTGGCGCA GCCCTCTGCT
    GAGCTCGGAT CTGAGAAGGA CACGGATACC GGATTGCCCC AGAAATCCAG TGCAATGCCC
    ACTAAGCATG CCAAGGTCTT GTTCCGGGGG AGTGGCGGTA AGCCCATCGC CGTGCTTTGC
    CTAACCGTGG AGACCCAGCC CCATGTGGTG GACCAGGTTT TCCGCTTCTA CCACCCAGAG
    CTCACCTTCC TGAAGAAGGC CATCCGCCTG CCTCCCTGGC ACACGCTTCC AGGTGCTCCC
    GTGGGAAGAC CTGGTGAGGA GCCCCCAGTC TATGTCCGAT GTAGCGACCC CAACGTCATC
    TGCGAGACCC AGAATGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36603
    Clone ID Related Accession (Same CDS sequence) XM_014113844.1 , XM_014113843.1 , XM_014113843.2
    Accession Version XM_014113844.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969319.1
    CDS129..4526
    Translation

    Target ORF information:

    RefSeq Version XM_014113844.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113844.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36603
    Clone ID Related Accession (Same CDS sequence) XM_014113844.1 , XM_014113843.1 , XM_014113843.2
    Accession Version XM_014113843.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969318.1
    CDS315..4712
    Translation

    Target ORF information:

    RefSeq Version XM_014113843.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36603
    Clone ID Related Accession (Same CDS sequence) XM_014113844.1 , XM_014113843.1 , XM_014113843.2
    Accession Version XM_014113843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969318.1
    CDS282..4679
    Translation

    Target ORF information:

    RefSeq Version XM_014113843.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGGGCCC CAGGGAGCCT CGGGACGTGT TCCTGAAGGT GGCCAGCGGT
    CCAAGCCCAG AGAGCAGAGA CTTCTTTGTC GCCGTTTACG CGGATCGTTG GCTGGCGACA
    CCCATCCAAA TATGGCAGGT CTACCTCCAC TCCTTGCAGC GTGTGGACGT CTCCTGTGTC
    ACAGGCCAGC GGACACGCCT ATCCCTTGTC CTTCGAGGGA CACGGACGGT TAGGAGAGTG
    CAGGCTTTCA CCTCACACCC TCAGGAGCTG AAGACTGACC CCAAAGGTGT GTTCGTGCTG
    CCACCACATG GGGTGCAGGA CCTGCATGTC GGTGTGAGGC CCCGCAGGGC AGGGAGCCGG
    TTCATCCACC TCAACCTGGT CGACGTGGAC TACCACCAGC TGGTGGCCTC ATGGCTCGTG
    TGCCTCTCCT GCCGCCCGCC TCTCATCTCC AAGGCTTTCG AGATCACACT GGCTGCAGGG
    AAAGGCAGAG GCGCCAACAA GCGGATCACC TACACCAACC CTTACCCCTC CCAGAGGACC
    TATCACCTGT ACAGCGACCA CCCTGACCTG CTGCAGTTCA GAGAGGACTC CTTCCAGATT
    GGGGGAGGAG AGACCTACAC CATCGGCCTG TGGTTCGCAC CTCGGCAGAG CCCCGGGGAG
    GAGGAGATTC TGATTTACAT CAATGACCAC GAAGACAAAA ATGAAGAAGC CTTCTGCGTC
    AAGGTCGTGT ACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36604
    Clone ID Related Accession (Same CDS sequence) XM_014113845.1
    Accession Version XM_014113845.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGGAGCCCCC CTCGGAGGGC
    AGGATCTCTC ACCTGGAGGC CCAGCTGAGC CAGGCGTCCT TGGTCCTGGG AGCCTCTGTT
    GCTGAACAGC TGCAGGAGCT GCCTTTTACG CCGGTGCACA CCCCGATTGT TGTGGGAGCC
    CAGACCAGGA GCTCTGGAAG TAAGCTCTCA CGAGCCTCAA TGGTGCTCCT GCAAGCCTGC
    GGCTTTCCTG AGATTCTGGA TGCCAATAAG CAACCAGCCG AGGCTGTAAA TCCTACAGAT
    CCCGTGAAAT TCAACCCCCA AAAGGAAGAA TCAGATTGTC TACAAAGCAA TGAGATAGTG
    CTGCATTTTC TTGCCTTTAG CAGAGTGTCC CAGGACTGCC AGGGAGCACC GTGGCCAAAG
    ACCGTGTATT TCACCTTCCA GTTCTATCGC TTCCCACCCG TGACAACGCC ACGCTTGCAG
    CTGATCAGGC TGGACGAGAC GGGCAGGACA GGCTCTGGTT CCCTGTTGTA TCTCCTGGTT
    CTCACTGACA AAGATGGCTC CTCTGATGCT GGGACACCTG GCTTTCAACT CAAGTACCTG
    GTGGAACCTG GGTTCCTGAA ACCCGGAGAA CAGCGCTGGT TCACCCACTA CATGGCTGCT
    CAGACCCTCC AGATCGACAT CTGGGATGGA GACTCCTTGC TGCTCGTGGG ATCTGCGGCC
    GTCCAGATGA AGCATCTCCT CCGCCAAGGC CGGCCGGCTG TGCAGGTCCT GCATGAGCTT
    GAGGTCGTGG CGACTGAATA TGAACAGGAT GTGGTGGTGA GTGGAGATGT GACCAGATTT
    GGCTGTGTCA AGCCCATCGG TGTCTACACA GTGGTAAAGG GCTGGCTGCA CCTGACGTTG
    GCCAACGTGG GTCATGTGTG TGAGCAGAGA GTGAGAGATT CCAGCTCATT GCCACCTTCC
    AGGTCACGGG TCATTTCGAA TGATGGAGCC AGACACTTCG AGGGAGGCAG TCTCATCTCG
    CGCGGGGGTC CGAGATCAGT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969320.1
    CDS315..4709
    Translation

    Target ORF information:

    RefSeq Version XM_014113845.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113845.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGGAGCCCCC CTCGGAGGGC
    AGGATCTCTC ACCTGGAGGC CCAGCTGAGC CAGGCGTCCT TGGTCCTGGG AGCCTCTGTT
    GCTGAACAGC TGCAGGAGCT GCCTTTTACG CCGGTGCACA CCCCGATTGT TGTGGGAGCC
    CAGACCAGGA GCTCTGGAAG TAAGCTCTCA CGAGCCTCAA TGGTGCTCCT GCAAGCCTGC
    GGCTTTCCTG AGATTCTGGA TGCCAATAAG CAACCAGCCG AGGCTGTAAA TCCTACAGAT
    CCCGTGAAAT TCAACCCCCA AAAGGAAGAA TCAGATTGTC TACAAAGCAA TGAGATAGTG
    CTGCATTTTC TTGCCTTTAG CAGAGTGTCC CAGGACTGCC AGGGAGCACC GTGGCCAAAG
    ACCGTGTATT TCACCTTCCA GTTCTATCGC TTCCCACCCG TGACAACGCC ACGCTTGCAG
    CTGATCAGGC TGGACGAGAC GGGCAGGACA GGCTCTGGTT CCCTGTTGTA TCTCCTGGTT
    CTCACTGACA AAGATGGCTC CTCTGATGCT GGGACACCTG GCTTTCAACT CAAGTACCTG
    GTGGAACCTG GGTTCCTGAA ACCCGGAGAA CAGCGCTGGT TCACCCACTA CATGGCTGCT
    CAGACCCTCC AGATCGACAT CTGGGATGGA GACTCCTTGC TGCTCGTGGG ATCTGCGGCC
    GTCCAGATGA AGCATCTCCT CCGCCAAGGC CGGCCGGCTG TGCAGGTCCT GCATGAGCTT
    GAGGTCGTGG CGACTGAATA TGAACAGGAT GTGGTGGTGA GTGGAGATGT GACCAGATTT
    GGCTGTGTCA AGCCCATCGG TGTCTACACA GTGGTAAAGG GCTGGCTGCA CCTGACGTTG
    GCCAACGTGG GTCATGTGTG TGAGCAGAGA GTGAGAGATT CCAGCTCATT GCCACCTTCC
    AGGTCACGGG TCATTTCGAA TGATGGAGCC AGACACTTCG AGGGAGGCAG TCTCATCTCG
    CGCGGGGGTC CGAGATCAGT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36605
    Clone ID Related Accession (Same CDS sequence) XM_014113846.1
    Accession Version XM_014113846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969321.1
    CDS315..4709
    Translation

    Target ORF information:

    RefSeq Version XM_014113846.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36606
    Clone ID Related Accession (Same CDS sequence) XM_014113847.1
    Accession Version XM_014113847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4395bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969322.1
    CDS315..4709
    Translation

    Target ORF information:

    RefSeq Version XM_014113847.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36607
    Clone ID Related Accession (Same CDS sequence) XM_014113848.1 , XM_014113848.2 , XM_014113850.1
    Accession Version XM_014113848.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969323.1
    CDS315..4610
    Translation

    Target ORF information:

    RefSeq Version XM_014113848.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36607
    Clone ID Related Accession (Same CDS sequence) XM_014113848.1 , XM_014113848.2 , XM_014113850.1
    Accession Version XM_014113848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969323.1
    CDS282..4577
    Translation

    Target ORF information:

    RefSeq Version XM_014113848.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36607
    Clone ID Related Accession (Same CDS sequence) XM_014113848.1 , XM_014113848.2 , XM_014113850.1
    Accession Version XM_014113850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969325.1
    CDS130..4425
    Translation

    Target ORF information:

    RefSeq Version XM_014113850.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    CAGGAGCCCC CCTCGGAGGG CAGGATCTCT CACCTGGAGG CCCAGCTGAG CCAGGCGTCC
    TTGGTCCTGG GAGCCTCTGT TGCTGAACAG CTGCAGGAGC TGCCTTTTAC GCCGGTGCAC
    ACCCCGATTG TTGTGGGAGC CCAGACCAGG AGCTCTGGAA GTAAGCTCTC ACGAGCCTCA
    ATGGTGCTCC TGCAAGCCTG CGGCTTTCCT GAGATTCTGG ATGCCAATAA GCAACCAGCC
    GAGGCTGTAA ATCCTACAGA TCCCGTGAAA TTCAACCCCC AAAAGGAAGA ATCAGATTGT
    CTACAAAGCA ATGAGATAGT GCTGCATTTT CTTGCCTTTA GCAGAGTGTC CCAGGACTGC
    CAGGGAGCAC CGTGGCCAAA GACCGTGTAT TTCACCTTCC AGTTCTATCG CTTCCCACCC
    GTGACAACGC CACGCTTGCA GCTGATCAGG CTGGACGAGA CGGGCAGGAC AGGCTCTGGT
    TCCCTGTTGT ATCTCCTGGT TCTCACTGAC AAAGATGGCT CCTCTGATGC TGGGACACCT
    GGCTTTCAAC TCAAGTACCT GGTGGAACCT GGGTTCCTGA AACCCGGAGA ACAGCGCTGG
    TTCACCCACT ACATGGCTGC TCAGACCCTC CAGATCGACA TCTGGGATGG AGACTCCTTG
    CTGCTCGTGG GATCTGCGGC CGTCCAGATG AAGCATCTCC TCCGCCAAGG CCGGCCGGCT
    GTGCAGGTCC TGCATGAGCT TGAGGTCGTG GCGACTGAAT ATGAACAGGA TGTGGTGGTG
    AGTGGAGATG TGACCAGATT TGGCTGTGTC AAGCCCATCG GTGTCTACAC AGTGGTAAAG
    GGCTGGCTGC ACCTGACGTT GGCCAACGTG GGTCATGTGT GTGAGCAGAG AGTGAGAGAT
    TCCAGCTCAT TGCCACCTTC CAGGTCACGG GTCATTTCGA ATGATGGAGC CAGACACTTC
    GAGGGAGGCA GTCTCATCTC GCGCGGGGGT CCGAGATCAG TAAAAAATGT TGTCCAAGCA
    CAGAAGCTGG CTGACGTGGA CCGTGAGCTG GCCGCCATGC TGTTCACCCA CATGCAACAT
    GGGAGCAAGG GGCCCCAAGG TGCTTGCCGT GAGGGGGATG CCGTCCGCAG GCGCAAGCTG
    GAGCGGATGA GGTCCGTGCG CCTTCAAGAG TCCAGAGGAG AGCTCAGCTG CTGGGGGGCC
    AGCATGTTGA TTCAGCAGAA TATCCGCGCA CAGCACGCAC GTGACCTGCA GGTCATTGCT
    GCCTATCGGG AGCGTACGAA GGCAGAGAGC ATCACCAGTG CGCTGAGCCT GGCCATCACC
    ACGATGCACA CAATCCACGT CACACTGGGC ACTGCCGAGT TCTTCGAGTT TGCCCTTAGG
    AACCCCCACA ACACACAGCA CACAGTGACC ATTGAGGTGG ACAATCCTGA GCTCAGCATC
    ATCCAGGACA GTCAGGAGTG GAGATACTTC AAAGACGCGA CCAAGCTGCA CACGCCGGTG
    GAGGAGGACA TGTTCCATCT GCGGGGCAGC CTTGCCCCGC AGATCTTCCT GCGCCCACGA
    GAGACCGCTC ACATCCCCTT CAAATACCAG ACCTTCTCTG TAGGGCAACT GGCCCTGGCG
    CAGCCCTCTG CTGAGCTCGG ATCTGAGAAG GACACGGATA CCGGATTGCC CCAGAAATCC
    AGTGCAATGC CCACTAAGCA TGCCAAGGTC TTGTTCCGGG GGAGTGGCGG TAAGCCCATC
    GCCGTGCTTT GCCTAACCGT GGAGACCCAG CCCCATGTGG TGGACCAGGT TTTCCGCTTC
    TACCACCCAG AGCTCACCTT CCTGAAGAAG GCCATCCGCC TGCCTCCCTG GCACACGCTT
    CCAGCAGGTG CTCCCGTGGG AAGACCTGGT GAGGAGCCCC CAGTCTATGT CCGATGTAGC
    GACCCCAACG TCATCTGCGA GACCCAGAAT GTGGGCCCCA GGGAGCCTCG GGACGTGTTC
    CTGAAGGTGG CCAGCGGTCC AAGCCCAGAG AGCAGAGACT TCTTTGTCGC CGTTTACGCG
    GATCGTTGGC TGGCGACACC CATCCAAATA TGGCAGGTCT ACCTCCACTC CTTGCAGCGT
    GTGGACGTCT CCTGTGTCAC AGGCCAGCGG ACACGCCTAT CCCTTGTCCT TCGAGGGACA
    CGGACGGTTA GGAGAGTGCA GGCTTTCACC TCACACCCTC AGGAGCTGAA GACTGACCCC
    AAAGGTGTGT TCGTGCTGCC ACCACATGGG GTGCAGGACC TGCATGTCGG TGTGAGGCCC
    CGCAGGGCAG GGAGCCGGTT CATCCACCTC AACCTGGTCG ACGTGGACTA CCACCAGCTG
    GTGGCCTCAT GGCTCGTGTG CCTCTCCTGC CGCCCGCCTC TCATCTCCAA GGCTTTCGAG
    ATCACACTGG CTGCAGGGAA AGGCAGAGGC GCCAACAAGC GGATCACCTA CACCAACCCT
    TACCCCTCCC AGAGGACCTA TCACCTGTAC AGCGACCACC CTGACCTGCT GCAGTTCAGA
    GAGGACTCCT TCCAGATTGG GGGAGGAGAG ACCTACACCA TCGGCCTGTG GTTCGCACCT
    CGGCAGAGCC CCGGGGAGGA GGAGATTCTG ATTTACATCA ATGACCACGA AGACAAAAAT
    GAAGAAGCCT TCTGCGTCAA GGTCGTGTAC CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36608
    Clone ID Related Accession (Same CDS sequence) XM_014113849.1
    Accession Version XM_014113849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GTGCAGCAAG TGGAGTCAGG GACTGTCCAG
    TTCCACTTCT CGCTGAGCCC TGAGGGACAC CTGGACACAC CCACGGGGCT GGTCCACAGC
    CCTGAGGCGG AGCGGCAGCC CTCCAGGAAG CCACCCACAT CTCCTGCAAA CCTGCCAGCA
    CTGCCGCCCC AGGGGCTCGC TGCCCTCCAG GACTCGCCGG TGGGACCAGG GTTGTCCCTT
    TCCCAGCTCG CAGCTTCCCC ACAGCCTACC ACCCGCTCCC GCTCAGCCAC ACCCCCTCCA
    CGGCAACCCG ACAGCTCTAA GCCCCCGCCG GCCCAGGAGC AGGAGCCCCC CTCGGAGGGC
    AGGATCTCTC ACCTGGAGGC CCAGCTGAGC CAGGCGTCCT TGGTCCTGGG AGCCTCTGTT
    GCTGAACAGC TGCAGGAGCT GCCTTTTACG CCGGTGCACA CCCCGATTGT TGTGGGAGCC
    CAGACCAGGA GCTCTGGAAG TAAGCTCTCA CGAGCCTCAA TGGTGCTCCT GCAAGCCTGC
    GGCTTTCCTG AGATTCTGGA TGCCAATAAG CAACCAGCCG AGGCTGTAAA TCCTACAGAT
    CCCGTGAAAT TCAACCCCCA AAAGGAAGAA TCAGATTGTC TACAAAGCAA TGAGATAGTG
    CTGCATTTTC TTGCCTTTAG CAGAGTGTCC CAGGACTGCC AGGGAGCACC GTGGCCAAAG
    ACCGTGTATT TCACCTTCCA GTTCTATCGC TTCCCACCCG TGACAACGCC ACGCTTGCAG
    CTGATCAGGC TGGACGAGAC GGGCAGGACA GGCTCTGGTT CCCTGTTGTA TCTCCTGGTT
    CTCACTGACA AAGATGGCTC CTCTGATGCT GGGACACCTG GCTTTCAACT CAAGTACCTG
    GTGGAACCTG GGTTCCTGAA ACCCGGAGAA CAGCGCTGGT TCACCCACTA CATGGCTGCT
    CAGACCCTCC AGATCGACAT CTGGGATGGA GACTCCTTGC TGCTCGTGGG ATCTGCGGCC
    GTCCAGATGA AGCATCTCCT CCGCCAAGGC CGGCCGGCTG TGCAGGTCCT GCATGAGCTT
    GAGGTCGTGG CGACTGAATA TGAACAGGAT GTGGTGGTGA GTGGAGATGT GACCAGATTT
    GGCTGTGTCA AGCCCATCGG TGTCTACACA GTGGTAAAGG GCTGGCTGCA CCTGACGTTG
    GCCAACGTGG GTCATGTGTG TGAGCAGAGA GTGAGAGATT CCAGCTCATT GCCACCTTCC
    AGGTCACGGG TCATTTCGAA TGATGGAGCC AGACACTTCG AGGGAGGCAG TCTCATCTCG
    CGCGGGGGTC CGAGATCAGT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969324.1
    CDS315..4529
    Translation

    Target ORF information:

    RefSeq Version XM_014113849.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GTGCAGCAAG TGGAGTCAGG GACTGTCCAG
    TTCCACTTCT CGCTGAGCCC TGAGGGACAC CTGGACACAC CCACGGGGCT GGTCCACAGC
    CCTGAGGCGG AGCGGCAGCC CTCCAGGAAG CCACCCACAT CTCCTGCAAA CCTGCCAGCA
    CTGCCGCCCC AGGGGCTCGC TGCCCTCCAG GACTCGCCGG TGGGACCAGG GTTGTCCCTT
    TCCCAGCTCG CAGCTTCCCC ACAGCCTACC ACCCGCTCCC GCTCAGCCAC ACCCCCTCCA
    CGGCAACCCG ACAGCTCTAA GCCCCCGCCG GCCCAGGAGC AGGAGCCCCC CTCGGAGGGC
    AGGATCTCTC ACCTGGAGGC CCAGCTGAGC CAGGCGTCCT TGGTCCTGGG AGCCTCTGTT
    GCTGAACAGC TGCAGGAGCT GCCTTTTACG CCGGTGCACA CCCCGATTGT TGTGGGAGCC
    CAGACCAGGA GCTCTGGAAG TAAGCTCTCA CGAGCCTCAA TGGTGCTCCT GCAAGCCTGC
    GGCTTTCCTG AGATTCTGGA TGCCAATAAG CAACCAGCCG AGGCTGTAAA TCCTACAGAT
    CCCGTGAAAT TCAACCCCCA AAAGGAAGAA TCAGATTGTC TACAAAGCAA TGAGATAGTG
    CTGCATTTTC TTGCCTTTAG CAGAGTGTCC CAGGACTGCC AGGGAGCACC GTGGCCAAAG
    ACCGTGTATT TCACCTTCCA GTTCTATCGC TTCCCACCCG TGACAACGCC ACGCTTGCAG
    CTGATCAGGC TGGACGAGAC GGGCAGGACA GGCTCTGGTT CCCTGTTGTA TCTCCTGGTT
    CTCACTGACA AAGATGGCTC CTCTGATGCT GGGACACCTG GCTTTCAACT CAAGTACCTG
    GTGGAACCTG GGTTCCTGAA ACCCGGAGAA CAGCGCTGGT TCACCCACTA CATGGCTGCT
    CAGACCCTCC AGATCGACAT CTGGGATGGA GACTCCTTGC TGCTCGTGGG ATCTGCGGCC
    GTCCAGATGA AGCATCTCCT CCGCCAAGGC CGGCCGGCTG TGCAGGTCCT GCATGAGCTT
    GAGGTCGTGG CGACTGAATA TGAACAGGAT GTGGTGGTGA GTGGAGATGT GACCAGATTT
    GGCTGTGTCA AGCCCATCGG TGTCTACACA GTGGTAAAGG GCTGGCTGCA CCTGACGTTG
    GCCAACGTGG GTCATGTGTG TGAGCAGAGA GTGAGAGATT CCAGCTCATT GCCACCTTCC
    AGGTCACGGG TCATTTCGAA TGATGGAGCC AGACACTTCG AGGGAGGCAG TCTCATCTCG
    CGCGGGGGTC CGAGATCAGT AAAAAATGTT GTCCAAGCAC AGAAGCTGGC TGACGTGGAC
    CGTGAGCTGG CCGCCATGCT GTTCACCCAC ATGCAACATG GGAGCAAGGG GCCCCAAGGT
    GCTTGCCGTG AGGGGGATGC CGTCCGCAGG CGCAAGCTGG AGCGGATGAG GTCCGTGCGC
    CTTCAAGAGT CCAGAGGAGA GCTCAGCTGC TGGGGGGCCA GCATGTTGAT TCAGCAGAAT
    ATCCGCGCAC AGCACGCACG TGACCTGCAG GTCATTGCTG CCTATCGGGA GCGTACGAAG
    GCAGAGAGCA TCACCAGTGC GCTGAGCCTG GCCATCACCA CGATGCACAC AATCCACGTC
    ACACTGGGCA CTGCCGAGTT CTTCGAGTTT GCCCTTAGGA ACCCCCACAA CACACAGCAC
    ACAGTGACCA TTGAGGTGGA CAATCCTGAG CTCAGCATCA TCCAGGACAG TCAGGAGTGG
    AGATACTTCA AAGACGCGAC CAAGCTGCAC ACGCCGGTGG AGGAGGACAT GTTCCATCTG
    CGGGGCAGCC TTGCCCCGCA GATCTTCCTG CGCCCACGAG AGACCGCTCA CATCCCCTTC
    AAATACCAGA CCTTCTCTGT AGGGCAACTG GCCCTGGCGC AGCCCTCTGC TGAGCTCGGA
    TCTGAGAAGG ACACGGATAC CGGATTGCCC CAGAAATCCA GTGCAATGCC CACTAAGCAT
    GCCAAGGTCT TGTTCCGGGG GAGTGGCGGT AAGCCCATCG CCGTGCTTTG CCTAACCGTG
    GAGACCCAGC CCCATGTGGT GGACCAGGTT TTCCGCTTCT ACCACCCAGA GCTCACCTTC
    CTGAAGAAGG CCATCCGCCT GCCTCCCTGG CACACGCTTC CAGCAGGTGC TCCCGTGGGA
    AGACCTGGTG AGGAGCCCCC AGTCTATGTC CGATGTAGCG ACCCCAACGT CATCTGCGAG
    ACCCAGAATG TGGGCCCCAG GGAGCCTCGG GACGTGTTCC TGAAGGTGGC CAGCGGTCCA
    AGCCCAGAGA GCAGAGACTT CTTTGTCGCC GTTTACGCGG ATCGTTGGCT GGCGACACCC
    ATCCAAATAT GGCAGGTCTA CCTCCACTCC TTGCAGCGTG TGGACGTCTC CTGTGTCACA
    GGCCAGCGGA CACGCCTATC CCTTGTCCTT CGAGGGACAC GGACGGTTAG GAGAGTGCAG
    GCTTTCACCT CACACCCTCA GGAGCTGAAG ACTGACCCCA AAGGTGTGTT CGTGCTGCCA
    CCACATGGGG TGCAGGACCT GCATGTCGGT GTGAGGCCCC GCAGGGCAGG GAGCCGGTTC
    ATCCACCTCA ACCTGGTCGA CGTGGACTAC CACCAGCTGG TGGCCTCATG GCTCGTGTGC
    CTCTCCTGCC GCCCGCCTCT CATCTCCAAG GCTTTCGAGA TCACACTGGC TGCAGGGAAA
    GGCAGAGGCG CCAACAAGCG GATCACCTAC ACCAACCCTT ACCCCTCCCA GAGGACCTAT
    CACCTGTACA GCGACCACCC TGACCTGCTG CAGTTCAGAG AGGACTCCTT CCAGATTGGG
    GGAGGAGAGA CCTACACCAT CGGCCTGTGG TTCGCACCTC GGCAGAGCCC CGGGGAGGAG
    GAGATTCTGA TTTACATCAA TGACCACGAA GACAAAAATG AAGAAGCCTT CTGCGTCAAG
    GTCGTGTACC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36609
    Clone ID Related Accession (Same CDS sequence) XM_014113851.1
    Accession Version XM_014113851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969326.1
    CDS315..3992
    Translation

    Target ORF information:

    RefSeq Version XM_014113851.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACCTCTC TTTCGAGGGC TGCAGAAGAG TATTCCCTTC CCTCAGCAGA GCCAGCAGAA 
    ACTGTACTCC TAGAGGCCGA AGTGACCCTC GGGACTGGCA GAATGAATGA CTGGCTCAGG
    ATTTTTGACC AAAACGTGCT GGTCCCTCCC CACCCACAGA GAGCACGCCA GCTTTTAAAG
    GAATCAACAG CATTTCAGTG TGTCCTGAAG TGGCTTGACG GGCCACTAAT TAAGCAGGGA
    GTGCTGGAGG TGCTTTCGGA GGTGGGCTGC CATCTGCGTG TGTCTCTCTT TGATGTCACC
    TACCGACACT TTTTCGGGAG GACATGGAAG ACCGCGGTGA GGCCGGCTGA GCAGCTTTCC
    AGGCAGCCGT CTAGGATCAT CTTTGATGAG CCCTTGTACT TTCACACGTC CTTGAACCAC
    CCTAACGTGG TGGCTGTGGT TGAGGTGGTC ACCGAAGGCC GGAAGCAGGA TGGCTCTCTC
    CAGGCTCTGT CATGTGGCTT TGGAATTCTT CGGATCTTTG GCAACAAACT GGAATCTCCT
    ACCTCTGCCT CCCAGGACAA GCGGTTGAGG CTGTATCACG GCACTCCCCG AGCCCTCCTG
    CACCCACTGC TCCAGGACCC TTTCGAACAA AATAAACACA TGACCCTCAT TGAGAGCTGC
    AGCCTGCAAT ACACTCTGAA GCCACACCCG CTCCTGGAGC CCGTGTTCCA TCTTCTTCCC
    CAGAACCTTC TGGTGTCTGG TCTGCAGCAC ATCCCTGGCC TCGTTCCAGC TCATGGAGAA
    AAGGGGGACA CCCTCCGAAA GCCTCGCCTT CAGAAGTCTG TCACATGGTA CTTGGATGAT
    TTATTCTTCA CGCTATATCC TTCCCTTGAG AAGTTTGAGG AAGAGCTTCT GGAACTCCTC
    ATCAGTGATA ACTTCCGGGA GGGGGACAGT CCACTAGATG GCAGCACGCT GGAGATCTTG
    GAGCGCCGCC TGTGTGTGGG CGTGCACAAT GGTCTGGGCT TCGTGCAGAG GCCACACGTG
    GTGGTGCTGG TGCCCGAGAT GGATGTGGCC TTGACGCGCT CTGCCAGTTT CAGCAGGAAA
    GTTGGCTCCT CTTCGAAGAC CAGCTCTGGA AACCAGGCTC TAGTTTTGAG AAGCCGCCTC
    CGACTCCCTG AGATGGTCCA TCACCCTGCG TTTGCCATAG TCTTTCAACT GGAGTACGTG
    TTCACCAGCC CTTCTGGAGT GGACAGCAAG GCAGCCTCGG TCACCTCTTT GTCCAGTGTG
    GCGTGTATGC ACATAGTTCG CTGGGCTGTT TGGAACCCCT TGCTGGAATC CGGCTCTGGG
    AAGGTGATCT TACCTCTGCA GGGTGGGATC CGGCCCAACC CCTCACACTG TTTGGTCTAT
    AAAGTAACCC CTGCCAGCAT GAGCTCTGAA GAGGTGCAGC AAGTGGAGTC AGGGACTGTC
    CAGTTCCACT TCTCGCTGAG CCCTGAGGGA CACCTGGACA CACCCACGGG GCTGGTCCAC
    AGCCCTGAGG CGGAGCGGCA GCCCTCCAGG AAGCCACCCA CATCTCCTGC AAACCTGCCA
    GCACTGCCGC CCCAGGGGCT CGCTGCCCTC CAGGACTCGC CGGTGGGACC AGGGTTGTCC
    CTTTCCCAGC TCGCAGCTTC CCCACAGCCT ACCACCCGCT CCCGCTCAGC CACACCCCCT
    CCACGGCAAC CCGACAGCTC TAAGCCCCCG CCGGCCCAGG AGCAGGAGCC CCCCTCGGAG
    GGCAGGATCT CTCACCTGGA GGCCCAGCTG AGCCAGGCGT CCTTGGTCCT GGGAGCCTCT
    GTTGCTGAAC AGCTGCAGGA GCTGCCTTTT ACGCCGGTGC ACACCCCGAT TGTTGTGGGA
    GCCCAGACCA GGAGCTCTGG AAGTAAGCTC TCACGAGCCT CAATGGTGCT CCTGCAAGCC
    TGCGGCTTTC CTGAGATTCT GGATGCCAAT AAGCAACCAG CCGAGGCTGT AAATCCTACA
    GATCCCGTGA AATTCAACCC CCAAAAGGAA GAATCAGATT GTCTACAAAG CAATGAGATA
    GTGCTGCATT TTCTTGCCTT TAGCAGAGTG TCCCAGGACT GCCAGGGAGC ACCGTGGCCA
    AAGACCGTGT ATTTCACCTT CCAGTTCTAT CGCTTCCCAC CCGTGACAAC GCCACGCTTG
    CAGCTGATCA GGCTGGACGA GACGGGCAGG ACAGGCTCTG GTTCCCTGTT GTATCTCCTG
    GTTCTCACTG ACAAAGATGG CTCCTCTGAT GCTGGGACAC CTGGCTTTCA ACTCAAGTAC
    CTGGTGGAAC CTGGGTTCCT GAAACCCGGA GAACAGCGCT GGTTCACCCA CTACATGGCT
    GCTCAGACCC TCCAGATCGA CATCTGGGAT GGAGACTCCT TGCTGCTCGT GGGATCTGCG
    GCCGTCCAGA TGAAGCATCT CCTCCGCCAA GGCCGGCCGG CTGTGCAGGT CCTGCATGAG
    CTTGAGGTCG TGGCGACTGA ATATGAACAG GATGTGGTGG TGAGTGGAGA TGTGACCAGA
    TTTGGCTGTG TCAAGCCCAT CGGTGTCTAC ACAGTGGTAA AGGGCTGGCT GCACCTGACG
    TTGGCCAACG TGGGTCATGT GTGTGAGCAG AGAGTGAGAG ATTCCAGCTC ATTGCCACCT
    TCCAGGTCAC GGGTCATTTC GAATGATGGA GCCAGACACT TCGAGGGAGG CAGTCTCATC
    TCGCGCGGGG GTCCGAGATC AGTAAAAAAT GTTGTCCAAG CACAGAAGCT GGCTGACGTG
    GACCGTGAGC TGGCCGCCAT GCTGTTCACC CACATGCAAC ATGGGAGCAA GGGGCCCCAA
    GGTGCTTGCC GTGAGGGGGA TGCCGTCCGC AGGCGCAAGC TGGAGCGGAT GAGGTCCGTG
    CGCCTTCAAG AGTCCAGAGG AGAGCTCAGC TGCTGGGGGG CCAGCATGTT GATTCAGCAG
    AATATCCGCG CACAGCACGC ACGTGACCTG CAGGTCATTG CTGCCTATCG GGAGCGTACG
    AAGGCAGAGA GCATCACCAG TGCGCTGAGC CTGGCCATCA CCACGATGCA CACAATCCAC
    GTCACACTGG GCACTGCCGA GTTCTTCGAG TTTGCCCTTA GGAACCCCCA CAACACACAG
    CACACAGTGA CCATTGAGGT GGACAATCCT GAGCTCAGCA TCATCCAGGA CAGTCAGGAG
    TGGAGATACT TCAAAGACGC GACCAAGCTG CACACGCCGG TGGAGGAGGA CATGTTCCAT
    CTGCGGGGCA GCCTTGCCCC GCAGATCTTC CTGCGCCCAC GAGAGACCGC TCACATCCCC
    TTCAAATACC AGACCTTCTC TGTAGGGCAA CTGGCCCTGG CGCAGCCCTC TGCTGAGCTC
    GGATCTGAGA AGGACACGGA TACCGGATTG CCCCAGAAAT CCAGTGCAAT GCCCACTAAG
    CATGCCAAGG TCTTGTTCCG GGGGAGTGGC GGTAAGCCCA TCGCCGTGCT TTGCCTAACC
    GTGGAGACCC AGCCCCATGT GGTGGACCAG GTTTTCCGCT TCTACCACCC AGAGCTCACC
    TTCCTGAAGA AGGCCATCCG CCTGCCTCCC TGGCACACGC TTCCAGCAGG TGCTCCCGTG
    GGAAGACCTG GTGAGGAGCC CCCAGTCTAT GTCCGATGTA GCGACCCCAA CGTCATCTGC
    GAGACCCAGA ATGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06756
    Clone ID Related Accession (Same CDS sequence) XM_005620413.1 , XM_005620413.2 , NM_001135788.1
    Accession Version NM_001135788.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU659993.1. On Oct 14, 2008 this sequence version replaced XM_546745.2. ##Evidence-Data-START## Transcript exon combination :: EU659993.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129260.1
    CDS110..4399
    Translation

    Target ORF information:

    RefSeq Version NM_001135788.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06756
    Clone ID Related Accession (Same CDS sequence) XM_005620413.1 , XM_005620413.2 , NM_001135788.1
    Accession Version XM_005620413.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrocystin-4 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726059.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620470.1
    CDS315..4604
    Translation

    Target ORF information:

    RefSeq Version XM_005620413.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephronophthisis 4 (NPHP4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620413.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06756
    Clone ID Related Accession (Same CDS sequence) XM_005620413.1 , XM_005620413.2 , NM_001135788.1
    Accession Version XM_005620413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrocystin-4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620470.1
    CDS281..4570
    Translation

    Target ORF information:

    RefSeq Version XM_005620413.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris nephrocystin 4 (NPHP4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGACT GGCTCAGGAT TTTTGACCAA AACGTGCTGG TCCCTCCCCA CCCACAGAGA 
    GCACGCCAGC TTTTAAAGGA ATCAACAGCA TTTCAGTGTG TCCTGAAGTG GCTTGACGGG
    CCACTAATTA AGCAGGGAGT GCTGGAGGTG CTTTCGGAGG TGGGCTGCCA TCTGCGTGTG
    TCTCTCTTTG ATGTCACCTA CCGACACTTT TTCGGGAGGA CATGGAAGAC CGCGGTGAGG
    CCGGCTGAGC AGCTTTCCAG GCAGCCGTCT AGGATCATCT TTGATGAGCC CTTGTACTTT
    CACACGTCCT TGAACCACCC TAACGTGGTG GCTGTGGTTG AGGTGGTCAC CGAAGGCCGG
    AAGCAGGATG GCTCTCTCCA GGCTCTGTCA TGTGGCTTTG GAATTCTTCG GATCTTTGGC
    AACAAACTGG AATCTCCTAC CTCTGCCTCC CAGGACAAGC GGTTGAGGCT GTATCACGGC
    ACTCCCCGAG CCCTCCTGCA CCCACTGCTC CAGGACCCTT TCGAACAAAA TAAACACATG
    ACCCTCATTG AGAGCTGCAG CCTGCAATAC ACTCTGAAGC CACACCCGCT CCTGGAGCCC
    GTGTTCCATC TTCTTCCCCA GAACCTTCTG GTGTCTGGTC TGCAGCACAT CCCTGGCCTC
    GTTCCAGCTC ATGGAGAAAA GGGGGACACC CTCCGAAAGC CTCGCCTTCA GAAGTCTGTC
    ACATGGTACT TGGATGATTT ATTCTTCACG CTATATCCTT CCCTTGAGAA GTTTGAGGAA
    GAGCTTCTGG AACTCCTCAT CAGTGATAAC TTCCGGGAGG GGGACAGTCC ACTAGATGGC
    AGCACGCTGG AGATCTTGGA GCGCCGCCTG TGTGTGGGCG TGCACAATGG TCTGGGCTTC
    GTGCAGAGGC CACACGTGGT GGTGCTGGTG CCCGAGATGG ATGTGGCCTT GACGCGCTCT
    GCCAGTTTCA GCAGGAAAGT TGGCTCCTCT TCGAAGACCA GCTCTGGAAA CCAGGCTCTA
    GTTTTGAGAA GCCGCCTCCG ACTCCCTGAG ATGGTCCATC ACCCTGCGTT TGCCATAGTC
    TTTCAACTGG AGTACGTGTT CACCAGCCCT TCTGGAGTGG ACAGCAAGGC AGCCTCGGTC
    ACCTCTTTGT CCAGTGTGGC GTGTATGCAC ATAGTTCGCT GGGCTGTTTG GAACCCCTTG
    CTGGAATCCG GCTCTGGGAA GGTGATCTTA CCTCTGCAGG GTGGGATCCG GCCCAACCCC
    TCACACTGTT TGGTCTATAA AGTAACCCCT GCCAGCATGA GCTCTGAAGA GGTGCAGCAA
    GTGGAGTCAG GGACTGTCCA GTTCCACTTC TCGCTGAGCC CTGAGGGACA CCTGGACACA
    CCCACGGGGC TGGTCCACAG CCCTGAGGCG GAGCGGCAGC CCTCCAGGAA GCCACCCACA
    TCTCCTGCAA ACCTGCCAGC ACTGCCGCCC CAGGGGCTCG CTGCCCTCCA GGACTCGCCG
    GTGGGACCAG GGTTGTCCCT TTCCCAGCTC GCAGCTTCCC CACAGCCTAC CACCCGCTCC
    CGCTCAGCCA CACCCCCTCC ACGGCAACCC GACAGCTCTA AGCCCCCGCC GGCCCAGGAG
    GAGCCCCCCT CGGAGGGCAG GATCTCTCAC CTGGAGGCCC AGCTGAGCCA GGCGTCCTTG
    GTCCTGGGAG CCTCTGTTGC TGAACAGCTG CAGGAGCTGC CTTTTACGCC GGTGCACACC
    CCGATTGTTG TGGGAGCCCA GACCAGGAGC TCTGGAAGTA AGCTCTCACG AGCCTCAATG
    GTGCTCCTGC AAGCCTGCGG CTTTCCTGAG ATTCTGGATG CCAATAAGCA ACCAGCCGAG
    GCTGTAAATC CTACAGATCC CGTGAAATTC AACCCCCAAA AGGAAGAATC AGATTGTCTA
    CAAAGCAATG AGATAGTGCT GCATTTTCTT GCCTTTAGCA GAGTGTCCCA GGACTGCCAG
    GGAGCACCGT GGCCAAAGAC CGTGTATTTC ACCTTCCAGT TCTATCGCTT CCCACCCGTG
    ACAACGCCAC GCTTGCAGCT GATCAGGCTG GACGAGACGG GCAGGACAGG CTCTGGTTCC
    CTGTTGTATC TCCTGGTTCT CACTGACAAA GATGGCTCCT CTGATGCTGG GACACCTGGC
    TTTCAACTCA AGTACCTGGT GGAACCTGGG TTCCTGAAAC CCGGAGAACA GCGCTGGTTC
    ACCCACTACA TGGCTGCTCA GACCCTCCAG ATCGACATCT GGGATGGAGA CTCCTTGCTG
    CTCGTGGGAT CTGCGGCCGT CCAGATGAAG CATCTCCTCC GCCAAGGCCG GCCGGCTGTG
    CAGGTCCTGC ATGAGCTTGA GGTCGTGGCG ACTGAATATG AACAGGATGT GGTGGTGAGT
    GGAGATGTGA CCAGATTTGG CTGTGTCAAG CCCATCGGTG TCTACACAGT GGTAAAGGGC
    TGGCTGCACC TGACGTTGGC CAACGTGGGT CATGTGTGTG AGCAGAGAGT GAGAGATTCC
    AGCTCATTGC CACCTTCCAG GTCACGGGTC ATTTCGAATG ATGGAGCCAG ACACTTCGAG
    GGAGGCAGTC TCATCTCGCG CGGGGGTCCG AGATCAGTAA AAAATGTTGT CCAAGCACAG
    AAGCTGGCTG ACGTGGACCG TGAGCTGGCC GCCATGCTGT TCACCCACAT GCAACATGGG
    AGCAAGGGGC CCCAAGGTGC TTGCCGTGAG GGGGATGCCG TCCGCAGGCG CAAGCTGGAG
    CGGATGAGGT CCGTGCGCCT TCAAGAGTCC AGAGGAGAGC TCAGCTGCTG GGGGGCCAGC
    ATGTTGATTC AGCAGAATAT CCGCGCACAG CACGCACGTG ACCTGCAGGT CATTGCTGCC
    TATCGGGAGC GTACGAAGGC AGAGAGCATC ACCAGTGCGC TGAGCCTGGC CATCACCACG
    ATGCACACAA TCCACGTCAC ACTGGGCACT GCCGAGTTCT TCGAGTTTGC CCTTAGGAAC
    CCCCACAACA CACAGCACAC AGTGACCATT GAGGTGGACA ATCCTGAGCT CAGCATCATC
    CAGGACAGTC AGGAGTGGAG ATACTTCAAA GACGCGACCA AGCTGCACAC GCCGGTGGAG
    GAGGACATGT TCCATCTGCG GGGCAGCCTT GCCCCGCAGA TCTTCCTGCG CCCACGAGAG
    ACCGCTCACA TCCCCTTCAA ATACCAGACC TTCTCTGTAG GGCAACTGGC CCTGGCGCAG
    CCCTCTGCTG AGCTCGGATC TGAGAAGGAC ACGGATACCG GATTGCCCCA GAAATCCAGT
    GCAATGCCCA CTAAGCATGC CAAGGTCTTG TTCCGGGGGA GTGGCGGTAA GCCCATCGCC
    GTGCTTTGCC TAACCGTGGA GACCCAGCCC CATGTGGTGG ACCAGGTTTT CCGCTTCTAC
    CACCCAGAGC TCACCTTCCT GAAGAAGGCC ATCCGCCTGC CTCCCTGGCA CACGCTTCCA
    GGTGCTCCCG TGGGAAGACC TGGTGAGGAG CCCCCAGTCT ATGTCCGATG TAGCGACCCC
    AACGTCATCT GCGAGACCCA GAATGTGGGC CCCAGGGAGC CTCGGGACGT GTTCCTGAAG
    GTGGCCAGCG GTCCAAGCCC AGAGAGCAGA GACTTCTTTG TCGCCGTTTA CGCGGATCGT
    TGGCTGGCGA CACCCATCCA AATATGGCAG GTCTACCTCC ACTCCTTGCA GCGTGTGGAC
    GTCTCCTGTG TCACAGGCCA GCGGACACGC CTATCCCTTG TCCTTCGAGG GACACGGACG
    GTTAGGAGAG TGCAGGCTTT CACCTCACAC CCTCAGGAGC TGAAGACTGA CCCCAAAGGT
    GTGTTCGTGC TGCCACCACA TGGGGTGCAG GACCTGCATG TCGGTGTGAG GCCCCGCAGG
    GCAGGGAGCC GGTTCATCCA CCTCAACCTG GTCGACGTGG ACTACCACCA GCTGGTGGCC
    TCATGGCTCG TGTGCCTCTC CTGCCGCCCG CCTCTCATCT CCAAGGCTTT CGAGATCACA
    CTGGCTGCAG GGAAAGGCAG AGGCGCCAAC AAGCGGATCA CCTACACCAA CCCTTACCCC
    TCCCAGAGGA CCTATCACCT GTACAGCGAC CACCCTGACC TGCTGCAGTT CAGAGAGGAC
    TCCTTCCAGA TTGGGGGAGG AGAGACCTAC ACCATCGGCC TGTGGTTCGC ACCTCGGCAG
    AGCCCCGGGG AGGAGGAGAT TCTGATTTAC ATCAATGACC ACGAAGACAA AAATGAAGAA
    GCCTTCTGCG TCAAGGTCGT GTACCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.