AFAP1 cDNA ORF clone, Canis lupus familiaris(dog)

The following AFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe04399 XM_005618456.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe04401 XM_005618460.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe04402 XM_005618461.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OCe287292 XM_022417059.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe287293 XM_022417060.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe287294 XM_022417061.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe287295 XM_022417062.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe287296 XM_022417063.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe287297 XM_022417064.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe35770 XM_014112506.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112505.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112504.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112503.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112502.2
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35771 XM_014112507.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35772 XM_014112508.1
Latest version!
Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OCe35770 XM_014112505.1 Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112504.1 Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112506.1 Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112502.1 Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe35770 XM_014112503.1 Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe04399
    Clone ID Related Accession (Same CDS sequence) XM_005618456.2
    Accession Version XM_005618456.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618456.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG TGGGACCCCG AAGACGGCCC TCCTGCTTCC
    TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT TATGATAACG CGGTCCTGTA CGATAACTTG
    CCGTCTCCGA AAATCTTTGC TCGCTACCCA CCTGCTGACA GAAAGGCTTC TAGGCCGTCT
    CCTGACAAAC TGTCCTGTAA CCATTACAAA TGCCCTCCCT CCTGCGGTCT GCCTCTCATT
    CAGTCTGCCA CTAACCCCTC TTCTCTGGGG AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618513.1
    CDS472..2928
    Translation

    Target ORF information:

    RefSeq Version XM_005618456.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618456.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG TGGGACCCCG AAGACGGCCC TCCTGCTTCC
    TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT TATGATAACG CGGTCCTGTA CGATAACTTG
    CCGTCTCCGA AAATCTTTGC TCGCTACCCA CCTGCTGACA GAAAGGCTTC TAGGCCGTCT
    CCTGACAAAC TGTCCTGTAA CCATTACAAA TGCCCTCCCT CCTGCGGTCT GCCTCTCATT
    CAGTCTGCCA CTAACCCCTC TTCTCTGGGG AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04401
    Clone ID Related Accession (Same CDS sequence) XM_005618460.2
    Accession Version XM_005618460.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2283bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGGTGAAGG GTAAAAAGCC CCCAGTAGCC TCTAGTGGGG TTACGGGGAA AGGGAAGACT
    CTGAGCAGCC AGCAAAAAAA GGTAGATTCC ACAGCCGGTG TGAAGCGAAC ATCTTCAAAT
    GCTGACCAGT ACAAATATGG CAAGAATCGG GTTGAAGCCG ATGCCAAGCG GTTGCAGAGC
    AAAGAAGATG AGCTGCTAAA GCGTAAAGAA ACCCTGCGGA ACAGGCTGGC CCAGCTCCGG
    AAAGAGAGGA GAGACCTGAG GGCTGCCATC GAAGTAAACG CTGGCAGGAA GACGCAGGTG
    GTTCTGGAAG ACAAGCTGAA GAGGCTGGAG GAGGAGTGTA AGCAGAAGGA GGCGGAGCGT
    GTCAACCTGG AGCTGGAGCT GACGGAAGTC ACAGAGAGCC TGAAGAAGGC CCTGGCTGGC
    GGGGTCACCC TGGGGCTGGC CATCGAGCCC AGGTCAGGGA CATCGAGCCC ACAGTCCCCG
    GTTTTCAGGC ATCGCACCCT GGAGAGCTCA CCCATGTCCA GCTGTGAGAC CAGCGATGCG
    GAGGGCCCCG TGCCGGTGAA CAGTGCAGCC GTGCTGAGGA AGGGCCAGCC GGCCAGCGGC
    TCCCCCTGCC GGGGACATGT GCTGCGGAAG GCCAAGGAAT GGGAGCTGAA GAACGGCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618517.1
    CDS472..2754
    Translation

    Target ORF information:

    RefSeq Version XM_005618460.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618460.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGGTGAAGG GTAAAAAGCC CCCAGTAGCC TCTAGTGGGG TTACGGGGAA AGGGAAGACT
    CTGAGCAGCC AGCAAAAAAA GGTAGATTCC ACAGCCGGTG TGAAGCGAAC ATCTTCAAAT
    GCTGACCAGT ACAAATATGG CAAGAATCGG GTTGAAGCCG ATGCCAAGCG GTTGCAGAGC
    AAAGAAGATG AGCTGCTAAA GCGTAAAGAA ACCCTGCGGA ACAGGCTGGC CCAGCTCCGG
    AAAGAGAGGA GAGACCTGAG GGCTGCCATC GAAGTAAACG CTGGCAGGAA GACGCAGGTG
    GTTCTGGAAG ACAAGCTGAA GAGGCTGGAG GAGGAGTGTA AGCAGAAGGA GGCGGAGCGT
    GTCAACCTGG AGCTGGAGCT GACGGAAGTC ACAGAGAGCC TGAAGAAGGC CCTGGCTGGC
    GGGGTCACCC TGGGGCTGGC CATCGAGCCC AGGTCAGGGA CATCGAGCCC ACAGTCCCCG
    GTTTTCAGGC ATCGCACCCT GGAGAGCTCA CCCATGTCCA GCTGTGAGAC CAGCGATGCG
    GAGGGCCCCG TGCCGGTGAA CAGTGCAGCC GTGCTGAGGA AGGGCCAGCC GGCCAGCGGC
    TCCCCCTGCC GGGGACATGT GCTGCGGAAG GCCAAGGAAT GGGAGCTGAA GAACGGCACG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04402
    Clone ID Related Accession (Same CDS sequence) XM_005618461.2
    Accession Version XM_005618461.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618518.1
    CDS472..2661
    Translation

    Target ORF information:

    RefSeq Version XM_005618461.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618461.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287292
    Clone ID Related Accession (Same CDS sequence) XM_022417059.1
    Accession Version XM_022417059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCCTATGGA AGAAGCCCCT GCCCCTTGAT
    GAGGGCACCC CATCCCTGAA CCACTCGGGC ACAGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG TGGGACCCCG AAGACGGCCC TCCTGCTTCC
    TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT TATGATAACG CGGTCCTGTA CGATAACTTG
    CCGTCTCCGA AAATCTTTGC TCGCTACCCA CCTGCTGACA GAAAGGCTTC TAGGCCGTCT
    CCTGACAAAC TGTCCTGTAA CCATTACAAA TGCCCTCCCT CCTGCGGTCT GCCTCTCATT
    CAGTCTGCCA CTAACCCCTC TTCTCTGGGG AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272767.1
    CDS156..2732
    Translation

    Target ORF information:

    RefSeq Version XM_022417059.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCCTATGGA AGAAGCCCCT GCCCCTTGAT
    GAGGGCACCC CATCCCTGAA CCACTCGGGC ACAGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG TGGGACCCCG AAGACGGCCC TCCTGCTTCC
    TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT TATGATAACG CGGTCCTGTA CGATAACTTG
    CCGTCTCCGA AAATCTTTGC TCGCTACCCA CCTGCTGACA GAAAGGCTTC TAGGCCGTCT
    CCTGACAAAC TGTCCTGTAA CCATTACAAA TGCCCTCCCT CCTGCGGTCT GCCTCTCATT
    CAGTCTGCCA CTAACCCCTC TTCTCTGGGG AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287293
    Clone ID Related Accession (Same CDS sequence) XM_022417060.1
    Accession Version XM_022417060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCCTATGGA AGAAGCCCCT GCCCCTTGAT GAGGGCACCC CATCCCTGAA CCACTCGGGC
    ACAGGCTATC TCAACGTGCT CTCCAACAAC CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG
    GATAACAAGC TCATTTTCCA CAAGGACAGG ACTGATCTCA AGACCCACAT TGTTTCTATT
    CCTCTCCGCG GCTGTGATGT GATTCCGGGC TTGGACTCCA GACACCCCCT GACGTTCCGA
    CTGCTTCGCA ATGGACAGGA GGTGGCCGTG CTAGAGGCCT CCTCTTCTGA GGACATGGGC
    AGATGGATTG GGATTTTGCT GGCCGAGACA GGATCCTCTA CAGACCCTGG GGCCCTGCAC
    TACGACTATA TCGACGTGGA GACATCTGCG AACGTCATTC AGACAGCCAA ACAGACATTC
    TGTTTCATGA ACAGGCGCGG TATCTCTGCC AACCCGTACC TGGGGAGCAC CACCAACGGC
    TACGCCCACC CCAGTGGCAT GGCGCTGCAT TACGATGACG TCCCATGCAT TAACGGCTCG
    TGGGACCCCG AAGACGGCCC TCCTGCTTCC TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT
    TATGATAACG CGGTCCTGTA CGATAACTTG CCGTCTCCGA AAATCTTTGC TCGCTACCCA
    CCTGCTGACA GAAAGGCTTC TAGGCCGTCT CCTGACAAAC TGTCCTGTAA CCATTACAAA
    TGCCCTCCCT CCTGCGGTCT GCCTCTCATT CAGTCTGCCA CTAACCCCTC TTCTCTGGGG
    AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT
    GGGGTTACGG GGAAAGGGAA GACTCTGAGC AGCCAGCAAA AAAAGGTAGA TTCCACAGCC
    GGTGTGAAGC GAACATCTTC AAATGCTGAC CAGTACAAAT ATGGCAAGAA TCGGGTTGAA
    GCCGATGCCA AGCGGTTGCA GAGCAAAGAA GATGAGCTGC TAAAGCGTAA AGAAACCCTG
    CGGAACAGGC TGGCCCAGCT CCGGAAAGAG AGGAGAGACC TGAGGGCTGC CATCGAAGTA
    AACGCTGGCA GGAAGACGCA GGTGGTTCTG GAAGACAAGC TGAAGAGGCT GGAGGAGGAG
    TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC CTGGAGCTGG AGCTGACGGA AGTCACAGAG
    AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC ACCCTGGGGC TGGCCATCGA GCCCAGGTCA
    GGGACATCGA GCCCACAGTC CCCGGTTTTC AGGCATCGCA CCCTGGAGAG CTCACCCATG
    TCCAGCTGTG AGACCAGCGA TGCGGAGGGC CCCGTGCCGG TGAACAGTGC AGCCGTGCTG
    AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272768.1
    CDS151..2697
    Translation

    Target ORF information:

    RefSeq Version XM_022417060.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCCTATGGA AGAAGCCCCT GCCCCTTGAT GAGGGCACCC CATCCCTGAA CCACTCGGGC
    ACAGGCTATC TCAACGTGCT CTCCAACAAC CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG
    GATAACAAGC TCATTTTCCA CAAGGACAGG ACTGATCTCA AGACCCACAT TGTTTCTATT
    CCTCTCCGCG GCTGTGATGT GATTCCGGGC TTGGACTCCA GACACCCCCT GACGTTCCGA
    CTGCTTCGCA ATGGACAGGA GGTGGCCGTG CTAGAGGCCT CCTCTTCTGA GGACATGGGC
    AGATGGATTG GGATTTTGCT GGCCGAGACA GGATCCTCTA CAGACCCTGG GGCCCTGCAC
    TACGACTATA TCGACGTGGA GACATCTGCG AACGTCATTC AGACAGCCAA ACAGACATTC
    TGTTTCATGA ACAGGCGCGG TATCTCTGCC AACCCGTACC TGGGGAGCAC CACCAACGGC
    TACGCCCACC CCAGTGGCAT GGCGCTGCAT TACGATGACG TCCCATGCAT TAACGGCTCG
    TGGGACCCCG AAGACGGCCC TCCTGCTTCC TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT
    TATGATAACG CGGTCCTGTA CGATAACTTG CCGTCTCCGA AAATCTTTGC TCGCTACCCA
    CCTGCTGACA GAAAGGCTTC TAGGCCGTCT CCTGACAAAC TGTCCTGTAA CCATTACAAA
    TGCCCTCCCT CCTGCGGTCT GCCTCTCATT CAGTCTGCCA CTAACCCCTC TTCTCTGGGG
    AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT
    GGGGTTACGG GGAAAGGGAA GACTCTGAGC AGCCAGCAAA AAAAGGTAGA TTCCACAGCC
    GGTGTGAAGC GAACATCTTC AAATGCTGAC CAGTACAAAT ATGGCAAGAA TCGGGTTGAA
    GCCGATGCCA AGCGGTTGCA GAGCAAAGAA GATGAGCTGC TAAAGCGTAA AGAAACCCTG
    CGGAACAGGC TGGCCCAGCT CCGGAAAGAG AGGAGAGACC TGAGGGCTGC CATCGAAGTA
    AACGCTGGCA GGAAGACGCA GGTGGTTCTG GAAGACAAGC TGAAGAGGCT GGAGGAGGAG
    TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC CTGGAGCTGG AGCTGACGGA AGTCACAGAG
    AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC ACCCTGGGGC TGGCCATCGA GCCCAGGTCA
    GGGACATCGA GCCCACAGTC CCCGGTTTTC AGGCATCGCA CCCTGGAGAG CTCACCCATG
    TCCAGCTGTG AGACCAGCGA TGCGGAGGGC CCCGTGCCGG TGAACAGTGC AGCCGTGCTG
    AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287294
    Clone ID Related Accession (Same CDS sequence) XM_022417061.1
    Accession Version XM_022417061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2514bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCTATCTCA ACGTGCTCTC CAACAACCGC
    TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT AACAAGCTCA TTTTCCACAA GGACAGGACT
    GATCTCAAGA CCCACATTGT TTCTATTCCT CTCCGCGGCT GTGATGTGAT TCCGGGCTTG
    GACTCCAGAC ACCCCCTGAC GTTCCGACTG CTTCGCAATG GACAGGAGGT GGCCGTGCTA
    GAGGCCTCCT CTTCTGAGGA CATGGGCAGA TGGATTGGGA TTTTGCTGGC CGAGACAGGA
    TCCTCTACAG ACCCTGGGGC CCTGCACTAC GACTATATCG ACGTGGAGAC ATCTGCGAAC
    GTCATTCAGA CAGCCAAACA GACATTCTGT TTCATGAACA GGCGCGGTAT CTCTGCCAAC
    CCGTACCTGG GGAGCACCAC CAACGGCTAC GCCCACCCCA GTGGCATGGC GCTGCATTAC
    GATGACGTCC CATGCATTAA CGGCTCGTGG GACCCCGAAG ACGGCCCTCC TGCTTCCTGT
    GCCAGAGGCC TGGGAGAAGA GGTGCTTTAT GATAACGCGG TCCTGTACGA TAACTTGCCG
    TCTCCGAAAA TCTTTGCTCG CTACCCACCT GCTGACAGAA AGGCTTCTAG GCCGTCTCCT
    GACAAACTGT CCTGTAACCA TTACAAATGC CCTCCCTCCT GCGGTCTGCC TCTCATTCAG
    TCTGCCACTA ACCCCTCTTC TCTGGGGAGG GCGTCTCTCG GGCTCAGCTC TCAGGTGAAG
    GGTAAAAAGC CCCCAGTAGC CTCTAGTGGG GTTACGGGGA AAGGGAAGAC TCTGAGCAGC
    CAGCAAAAAA AGGTAGATTC CACAGCCGGT GTGAAGCGAA CATCTTCAAA TGCTGACCAG
    TACAAATATG GCAAGAATCG GGTTGAAGCC GATGCCAAGC GGTTGCAGAG CAAAGAAGAT
    GAGCTGCTAA AGCGTAAAGA AACCCTGCGG AACAGGCTGG CCCAGCTCCG GAAAGAGAGG
    AGAGACCTGA GGGCTGCCAT CGAAGTAAAC GCTGGCAGGA AGACGCAGGT GGTTCTGGAA
    GACAAGCTGA AGAGGCTGGA GGAGGAGTGT AAGCAGAAGG AGGCGGAGCG TGTCAACCTG
    GAGCTGGAGC TGACGGAAGT CACAGAGAGC CTGAAGAAGG CCCTGGCTGG CGGGGTCACC
    CTGGGGCTGG CCATCGAGCC CAGGTCAGGG ACATCGAGCC CACAGTCCCC GGTTTTCAGG
    CATCGCACCC TGGAGAGCTC ACCCATGTCC AGCTGTGAGA CCAGCGATGC GGAGGGCCCC
    GTGCCGGTGA ACAGTGCAGC CGTGCTGAGG AAGGGCCAGC CGGCCAGCGG CTCCCCCTGC
    CGGGGACATG TGCTGCGGAA GGCCAAGGAA TGGGAGCTGA AGAACGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272769.1
    CDS152..2665
    Translation

    Target ORF information:

    RefSeq Version XM_022417061.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCTATCTCA ACGTGCTCTC CAACAACCGC
    TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT AACAAGCTCA TTTTCCACAA GGACAGGACT
    GATCTCAAGA CCCACATTGT TTCTATTCCT CTCCGCGGCT GTGATGTGAT TCCGGGCTTG
    GACTCCAGAC ACCCCCTGAC GTTCCGACTG CTTCGCAATG GACAGGAGGT GGCCGTGCTA
    GAGGCCTCCT CTTCTGAGGA CATGGGCAGA TGGATTGGGA TTTTGCTGGC CGAGACAGGA
    TCCTCTACAG ACCCTGGGGC CCTGCACTAC GACTATATCG ACGTGGAGAC ATCTGCGAAC
    GTCATTCAGA CAGCCAAACA GACATTCTGT TTCATGAACA GGCGCGGTAT CTCTGCCAAC
    CCGTACCTGG GGAGCACCAC CAACGGCTAC GCCCACCCCA GTGGCATGGC GCTGCATTAC
    GATGACGTCC CATGCATTAA CGGCTCGTGG GACCCCGAAG ACGGCCCTCC TGCTTCCTGT
    GCCAGAGGCC TGGGAGAAGA GGTGCTTTAT GATAACGCGG TCCTGTACGA TAACTTGCCG
    TCTCCGAAAA TCTTTGCTCG CTACCCACCT GCTGACAGAA AGGCTTCTAG GCCGTCTCCT
    GACAAACTGT CCTGTAACCA TTACAAATGC CCTCCCTCCT GCGGTCTGCC TCTCATTCAG
    TCTGCCACTA ACCCCTCTTC TCTGGGGAGG GCGTCTCTCG GGCTCAGCTC TCAGGTGAAG
    GGTAAAAAGC CCCCAGTAGC CTCTAGTGGG GTTACGGGGA AAGGGAAGAC TCTGAGCAGC
    CAGCAAAAAA AGGTAGATTC CACAGCCGGT GTGAAGCGAA CATCTTCAAA TGCTGACCAG
    TACAAATATG GCAAGAATCG GGTTGAAGCC GATGCCAAGC GGTTGCAGAG CAAAGAAGAT
    GAGCTGCTAA AGCGTAAAGA AACCCTGCGG AACAGGCTGG CCCAGCTCCG GAAAGAGAGG
    AGAGACCTGA GGGCTGCCAT CGAAGTAAAC GCTGGCAGGA AGACGCAGGT GGTTCTGGAA
    GACAAGCTGA AGAGGCTGGA GGAGGAGTGT AAGCAGAAGG AGGCGGAGCG TGTCAACCTG
    GAGCTGGAGC TGACGGAAGT CACAGAGAGC CTGAAGAAGG CCCTGGCTGG CGGGGTCACC
    CTGGGGCTGG CCATCGAGCC CAGGTCAGGG ACATCGAGCC CACAGTCCCC GGTTTTCAGG
    CATCGCACCC TGGAGAGCTC ACCCATGTCC AGCTGTGAGA CCAGCGATGC GGAGGGCCCC
    GTGCCGGTGA ACAGTGCAGC CGTGCTGAGG AAGGGCCAGC CGGCCAGCGG CTCCCCCTGC
    CGGGGACATG TGCTGCGGAA GGCCAAGGAA TGGGAGCTGA AGAACGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287295
    Clone ID Related Accession (Same CDS sequence) XM_022417062.1
    Accession Version XM_022417062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCTATCTCA ACGTGCTCTC CAACAACCGC TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT
    AACAAGCTCA TTTTCCACAA GGACAGGACT GATCTCAAGA CCCACATTGT TTCTATTCCT
    CTCCGCGGCT GTGATGTGAT TCCGGGCTTG GACTCCAGAC ACCCCCTGAC GTTCCGACTG
    CTTCGCAATG GACAGGAGGT GGCCGTGCTA GAGGCCTCCT CTTCTGAGGA CATGGGCAGA
    TGGATTGGGA TTTTGCTGGC CGAGACAGGA TCCTCTACAG ACCCTGGGGC CCTGCACTAC
    GACTATATCG ACGTGGAGAC ATCTGCGAAC GTCATTCAGA CAGCCAAACA GACATTCTGT
    TTCATGAACA GGCGCGGTAT CTCTGCCAAC CCGTACCTGG GGAGCACCAC CAACGGCTAC
    GCCCACCCCA GTGGCATGGC GCTGCATTAC GATGACGTCC CATGCATTAA CGGCTCGTGG
    GACCCCGAAG ACGGCCCTCC TGCTTCCTGT GCCAGAGGCC TGGGAGAAGA GGTGCTTTAT
    GATAACGCGG TCCTGTACGA TAACTTGCCG TCTCCGAAAA TCTTTGCTCG CTACCCACCT
    GCTGACAGAA AGGCTTCTAG GCCGTCTCCT GACAAACTGT CCTGTAACCA TTACAAATGC
    CCTCCCTCCT GCGGTCTGCC TCTCATTCAG TCTGCCACTA ACCCCTCTTC TCTGGGGAGG
    GCGTCTCTCG GGCTCAGCTC TCAGGTGAAG GGTAAAAAGC CCCCAGTAGC CTCTAGTGGG
    GTTACGGGGA AAGGGAAGAC TCTGAGCAGC CAGCAAAAAA AGGTAGATTC CACAGCCGGT
    GTGAAGCGAA CATCTTCAAA TGCTGACCAG TACAAATATG GCAAGAATCG GGTTGAAGCC
    GATGCCAAGC GGTTGCAGAG CAAAGAAGAT GAGCTGCTAA AGCGTAAAGA AACCCTGCGG
    AACAGGCTGG CCCAGCTCCG GAAAGAGAGG AGAGACCTGA GGGCTGCCAT CGAAGTAAAC
    GCTGGCAGGA AGACGCAGGT GGTTCTGGAA GACAAGCTGA AGAGGCTGGA GGAGGAGTGT
    AAGCAGAAGG AGGCGGAGCG TGTCAACCTG GAGCTGGAGC TGACGGAAGT CACAGAGAGC
    CTGAAGAAGG CCCTGGCTGG CGGGGTCACC CTGGGGCTGG CCATCGAGCC CAGGTCAGGG
    ACATCGAGCC CACAGTCCCC GGTTTTCAGG CATCGCACCC TGGAGAGCTC ACCCATGTCC
    AGCTGTGAGA CCAGCGATGC GGAGGGCCCC GTGCCGGTGA ACAGTGCAGC CGTGCTGAGG
    AAGGGCCAGC CGGCCAGCGG CTCCCCCTGC CGGGGACATG TGCTGCGGAA GGCCAAGGAA
    TGGGAGCTGA AGAACGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272770.1
    CDS147..2630
    Translation

    Target ORF information:

    RefSeq Version XM_022417062.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCTATCTCA ACGTGCTCTC CAACAACCGC TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT
    AACAAGCTCA TTTTCCACAA GGACAGGACT GATCTCAAGA CCCACATTGT TTCTATTCCT
    CTCCGCGGCT GTGATGTGAT TCCGGGCTTG GACTCCAGAC ACCCCCTGAC GTTCCGACTG
    CTTCGCAATG GACAGGAGGT GGCCGTGCTA GAGGCCTCCT CTTCTGAGGA CATGGGCAGA
    TGGATTGGGA TTTTGCTGGC CGAGACAGGA TCCTCTACAG ACCCTGGGGC CCTGCACTAC
    GACTATATCG ACGTGGAGAC ATCTGCGAAC GTCATTCAGA CAGCCAAACA GACATTCTGT
    TTCATGAACA GGCGCGGTAT CTCTGCCAAC CCGTACCTGG GGAGCACCAC CAACGGCTAC
    GCCCACCCCA GTGGCATGGC GCTGCATTAC GATGACGTCC CATGCATTAA CGGCTCGTGG
    GACCCCGAAG ACGGCCCTCC TGCTTCCTGT GCCAGAGGCC TGGGAGAAGA GGTGCTTTAT
    GATAACGCGG TCCTGTACGA TAACTTGCCG TCTCCGAAAA TCTTTGCTCG CTACCCACCT
    GCTGACAGAA AGGCTTCTAG GCCGTCTCCT GACAAACTGT CCTGTAACCA TTACAAATGC
    CCTCCCTCCT GCGGTCTGCC TCTCATTCAG TCTGCCACTA ACCCCTCTTC TCTGGGGAGG
    GCGTCTCTCG GGCTCAGCTC TCAGGTGAAG GGTAAAAAGC CCCCAGTAGC CTCTAGTGGG
    GTTACGGGGA AAGGGAAGAC TCTGAGCAGC CAGCAAAAAA AGGTAGATTC CACAGCCGGT
    GTGAAGCGAA CATCTTCAAA TGCTGACCAG TACAAATATG GCAAGAATCG GGTTGAAGCC
    GATGCCAAGC GGTTGCAGAG CAAAGAAGAT GAGCTGCTAA AGCGTAAAGA AACCCTGCGG
    AACAGGCTGG CCCAGCTCCG GAAAGAGAGG AGAGACCTGA GGGCTGCCAT CGAAGTAAAC
    GCTGGCAGGA AGACGCAGGT GGTTCTGGAA GACAAGCTGA AGAGGCTGGA GGAGGAGTGT
    AAGCAGAAGG AGGCGGAGCG TGTCAACCTG GAGCTGGAGC TGACGGAAGT CACAGAGAGC
    CTGAAGAAGG CCCTGGCTGG CGGGGTCACC CTGGGGCTGG CCATCGAGCC CAGGTCAGGG
    ACATCGAGCC CACAGTCCCC GGTTTTCAGG CATCGCACCC TGGAGAGCTC ACCCATGTCC
    AGCTGTGAGA CCAGCGATGC GGAGGGCCCC GTGCCGGTGA ACAGTGCAGC CGTGCTGAGG
    AAGGGCCAGC CGGCCAGCGG CTCCCCCTGC CGGGGACATG TGCTGCGGAA GGCCAAGGAA
    TGGGAGCTGA AGAACGGCAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287296
    Clone ID Related Accession (Same CDS sequence) XM_022417063.1
    Accession Version XM_022417063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCCTATGGA AGAAGCCCCT GCCCCTTGAT
    GAGGGCACCC CATCCCTGAA CCACTCGGGC ACAGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272771.1
    CDS151..2460
    Translation

    Target ORF information:

    RefSeq Version XM_022417063.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTAACTT CTATCCTGAA TATTATCACC
    AAGGTGATCC GAGATGTCTA CAGTGGCTGC AGTGGGCCGG CGGATTCAGA AGGCCCACCC
    CCATCAAGCT CCCCAGTGCA CAAGACAGAG CTGGAGAAGA AACTGTCTTC TGAGAGACCC
    AGCTCTGATG GAGAGGGTGT TACAGAAAAT GGCATCACTG TGTGTAATGG AAAAGAACAA
    GTTAAGAGGA AAAAGAGTTC CAAATCAGAG GCCAAGGGCA CAGTGTCTAA AGTCACTGGG
    AAGAAGATCA CCAAGATCAT TGGCTTGGGG AAGAAAAAGC CATCAACAGA TGAGCAGACC
    TCATCAGCAG AGGAGGATGT CCCCACTTGT GGCCTATGGA AGAAGCCCCT GCCCCTTGAT
    GAGGGCACCC CATCCCTGAA CCACTCGGGC ACAGGCTATC TCAACGTGCT CTCCAACAAC
    CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACTGATCTCA AGACCCACAT TGTTTCTATT CCTCTCCGCG GCTGTGATGT GATTCCGGGC
    TTGGACTCCA GACACCCCCT GACGTTCCGA CTGCTTCGCA ATGGACAGGA GGTGGCCGTG
    CTAGAGGCCT CCTCTTCTGA GGACATGGGC AGATGGATTG GGATTTTGCT GGCCGAGACA
    GGATCCTCTA CAGACCCTGG GGCCCTGCAC TACGACTATA TCGACGTGGA GACATCTGCG
    AACGTCATTC AGACAGCCAA ACAGACATTC TGTTTCATGA ACAGGCGCGG TATCTCTGCC
    AACCCGTACC TGGGGAGCAC CACCAACGGC TACGCCCACC CCAGTGGCAT GGCGCTGCAT
    TACGATGACG TCCCATGCAT TAACGGCTCG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287297
    Clone ID Related Accession (Same CDS sequence) XM_022417064.1
    Accession Version XM_022417064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCTATCTCA ACGTGCTCTC CAACAACCGC TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT
    AACAAGCTCA TTTTCCACAA GGACAGGACT GATCTCAAGA CCCACATTGT TTCTATTCCT
    CTCCGCGGCT GTGATGTGAT TCCGGGCTTG GACTCCAGAC ACCCCCTGAC GTTCCGACTG
    CTTCGCAATG GACAGGAGGT GGCCGTGCTA GAGGCCTCCT CTTCTGAGGA CATGGGCAGA
    TGGATTGGGA TTTTGCTGGC CGAGACAGGA TCCTCTACAG ACCCTGGGGC CCTGCACTAC
    GACTATATCG ACGTGGAGAC ATCTGCGAAC GTCATTCAGA CAGCCAAACA GACATTCTGT
    TTCATGAACA GGCGCGGTAT CTCTGCCAAC CCGTACCTGG GGAGCACCAC CAACGGCTAC
    GCCCACCCCA GTGGCATGGC GCTGCATTAC GATGACGTCC CATGCATTAA CGGCTCGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272772.1
    CDS141..2357
    Translation

    Target ORF information:

    RefSeq Version XM_022417064.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAGCG GAAGCAGGTG TGAAGCAATG GAAGAGTTAA TAGTTGAACT CCGTCTCTTT 
    CTTGAACTCC TGGACCATGA ATATCTAACA TCAACTGTTA GAGAGAAAAA GGCAGTTATC
    ACCGACATCC TGTTGAGGAT ACAGTCATCT AAAGGTTTCG AAGTGAAGGA TCATGCTCAG
    AAGCAAGAGA CCCCCAATAG CCTGCCGGCT CCTCCCCAGA TGCCTTTGCC TGAGATCCCC
    CAGCCATGGC TGCCACCTGA CAGTGGGCCG CCACCGCTAC CAACATCCTC TCTCCCGGAA
    GGTTACTACG AGGAAGCTGT ACCACTGAGC CCTGGGAAAG CTCCGGAATA CATCACGTCA
    AATTATGACT CAGATGCAAT GAGTAGCTCT TATGAGTCTT ATGATGAGGA GGAAGAGGAT
    GGAAAGGGCA AGAAGACACG GCACCAGTGG CCGTCTGAGG AGGCCTCCAT GGACCTGGTG
    AAGGATGCCA AAATCTGTGC CTTCCTACTG AGGAAGAAGC GCTTCGGGCA GTGGAGCAAG
    CTTCTTTGTG TCGTCAAAGA CACCAAACTG CTGTGCTACA AAAGTTCCAA GGACCAACAG
    CCTCAGATGG AACTGCCGCT GCAGGGCTGC AACGTGACGT ACACACCGAA GGACAGTAAG
    AAGAAGAAAC ACGAGCTGAA AATCACCCAG CAGGGCACAG ACCCACTGGT CCTCGCCGTT
    CAGAGCAAGG AGCAGGCTGA GCAGTGGCTG AAGGTGATCC GAGATGTCTA CAGTGGCTGC
    AGTGGGCCGG CGGATTCAGA AGGCCCACCC CCATCAAGCT CCCCAGTGCA CAAGACAGAG
    CTGGAGAAGA AACTGTCTTC TGAGAGACCC AGCTCTGATG GAGAGGGTGT TACAGAAAAT
    GGCATCACTG TGTGTAATGG AAAAGAACAA GTTAAGAGGA AAAAGAGTTC CAAATCAGAG
    GCCAAGGGCA CAGTGTCTAA AGTCACTGGG AAGAAGATCA CCAAGATCAT TGGCTTGGGG
    AAGAAAAAGC CATCAACAGA TGAGCAGACC TCATCAGCAG AGGAGGATGT CCCCACTTGT
    GGCTATCTCA ACGTGCTCTC CAACAACCGC TGGCGGGAGC GCTGGTGCCG GGTCAAGGAT
    AACAAGCTCA TTTTCCACAA GGACAGGACT GATCTCAAGA CCCACATTGT TTCTATTCCT
    CTCCGCGGCT GTGATGTGAT TCCGGGCTTG GACTCCAGAC ACCCCCTGAC GTTCCGACTG
    CTTCGCAATG GACAGGAGGT GGCCGTGCTA GAGGCCTCCT CTTCTGAGGA CATGGGCAGA
    TGGATTGGGA TTTTGCTGGC CGAGACAGGA TCCTCTACAG ACCCTGGGGC CCTGCACTAC
    GACTATATCG ACGTGGAGAC ATCTGCGAAC GTCATTCAGA CAGCCAAACA GACATTCTGT
    TTCATGAACA GGCGCGGTAT CTCTGCCAAC CCGTACCTGG GGAGCACCAC CAACGGCTAC
    GCCCACCCCA GTGGCATGGC GCTGCATTAC GATGACGTCC CATGCATTAA CGGCTCGGTG
    AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT GGGGTTACGG GGAAAGGGAA GACTCTGAGC
    AGCCAGCAAA AAAAGGTAGA TTCCACAGCC GGTGTGAAGC GAACATCTTC AAATGCTGAC
    CAGTACAAAT ATGGCAAGAA TCGGGTTGAA GCCGATGCCA AGCGGTTGCA GAGCAAAGAA
    GATGAGCTGC TAAAGCGTAA AGAAACCCTG CGGAACAGGC TGGCCCAGCT CCGGAAAGAG
    AGGAGAGACC TGAGGGCTGC CATCGAAGTA AACGCTGGCA GGAAGACGCA GGTGGTTCTG
    GAAGACAAGC TGAAGAGGCT GGAGGAGGAG TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC
    CTGGAGCTGG AGCTGACGGA AGTCACAGAG AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC
    ACCCTGGGGC TGGCCATCGA GCCCAGGTCA GGGACATCGA GCCCACAGTC CCCGGTTTTC
    AGGCATCGCA CCCTGGAGAG CTCACCCATG TCCAGCTGTG AGACCAGCGA TGCGGAGGGC
    CCCGTGCCGG TGAACAGTGC AGCCGTGCTG AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC
    TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112502.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967977.1
    CDS371..2920
    Translation

    Target ORF information:

    RefSeq Version XM_014112502.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112502.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112504.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967979.1
    CDS356..2905
    Translation

    Target ORF information:

    RefSeq Version XM_014112504.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112505.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967980.1
    CDS285..2834
    Translation

    Target ORF information:

    RefSeq Version XM_014112505.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112503.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967978.1
    CDS304..2853
    Translation

    Target ORF information:

    RefSeq Version XM_014112503.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112503.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967980.1
    CDS183..2732
    Translation

    Target ORF information:

    RefSeq Version XM_014112505.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112506.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967981.1
    CDS236..2785
    Translation

    Target ORF information:

    RefSeq Version XM_014112506.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112504.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967979.1
    CDS369..2918
    Translation

    Target ORF information:

    RefSeq Version XM_014112504.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112506.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112506.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967981.1
    CDS91..2640
    Translation

    Target ORF information:

    RefSeq Version XM_014112506.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112506.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112502.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967977.1
    CDS473..3022
    Translation

    Target ORF information:

    RefSeq Version XM_014112502.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35770
    Clone ID Related Accession (Same CDS sequence) XM_014112502.2 , XM_014112504.1 , XM_014112505.1 , XM_014112503.2 , XM_014112505.2 , XM_014112506.1 , XM_014112504.2 , XM_014112506.2 , XM_014112502.1 , XM_014112503.1
    Accession Version XM_014112503.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967978.1
    CDS424..2973
    Translation

    Target ORF information:

    RefSeq Version XM_014112503.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT GATGAGGGCA CCCCATCCCT GAACCACTCG
    GGCACAGGCT ATCTCAACGT GCTCTCCAAC AACCGCTGGC GGGAGCGCTG GTGCCGGGTC
    AAGGATAACA AGCTCATTTT CCACAAGGAC AGGACTGATC TCAAGACCCA CATTGTTTCT
    ATTCCTCTCC GCGGCTGTGA TGTGATTCCG GGCTTGGACT CCAGACACCC CCTGACGTTC
    CGACTGCTTC GCAATGGACA GGAGGTGGCC GTGCTAGAGG CCTCCTCTTC TGAGGACATG
    GGCAGATGGA TTGGGATTTT GCTGGCCGAG ACAGGATCCT CTACAGACCC TGGGGCCCTG
    CACTACGACT ATATCGACGT GGAGACATCT GCGAACGTCA TTCAGACAGC CAAACAGACA
    TTCTGTTTCA TGAACAGGCG CGGTATCTCT GCCAACCCGT ACCTGGGGAG CACCACCAAC
    GGCTACGCCC ACCCCAGTGG CATGGCGCTG CATTACGATG ACGTCCCATG CATTAACGGC
    TCGTGGGACC CCGAAGACGG CCCTCCTGCT TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG
    CTTTATGATA ACGCGGTCCT GTACGATAAC TTGCCGTCTC CGAAAATCTT TGCTCGCTAC
    CCACCTGCTG ACAGAAAGGC TTCTAGGCCG TCTCCTGACA AACTGTCCTG TAACCATTAC
    AAATGCCCTC CCTCCTGCGG TCTGCCTCTC ATTCAGTCTG CCACTAACCC CTCTTCTCTG
    GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT
    AGTGGGGTTA CGGGGAAAGG GAAGACTCTG AGCAGCCAGC AAAAAAAGGT AGATTCCACA
    GCCGGTGTGA AGCGAACATC TTCAAATGCT GACCAGTACA AATATGGCAA GAATCGGGTT
    GAAGCCGATG CCAAGCGGTT GCAGAGCAAA GAAGATGAGC TGCTAAAGCG TAAAGAAACC
    CTGCGGAACA GGCTGGCCCA GCTCCGGAAA GAGAGGAGAG ACCTGAGGGC TGCCATCGAA
    GTAAACGCTG GCAGGAAGAC GCAGGTGGTT CTGGAAGACA AGCTGAAGAG GCTGGAGGAG
    GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC AACCTGGAGC TGGAGCTGAC GGAAGTCACA
    GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG GTCACCCTGG GGCTGGCCAT CGAGCCCAGG
    TCAGGGACAT CGAGCCCACA GTCCCCGGTT TTCAGGCATC GCACCCTGGA GAGCTCACCC
    ATGTCCAGCT GTGAGACCAG CGATGCGGAG GGCCCCGTGC CGGTGAACAG TGCAGCCGTG
    CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC CCCTGCCGGG GACATGTGCT GCGGAAGGCC
    AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35771
    Clone ID Related Accession (Same CDS sequence) XM_014112507.1
    Accession Version XM_014112507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT
    GATGAGGGCA CCCCATCCCT GAACCACTCG GGCACAGGCT ATCTCAACGT GCTCTCCAAC
    AACCGCTGGC GGGAGCGCTG GTGCCGGGTC AAGGATAACA AGCTCATTTT CCACAAGGAC
    AGGACTGATC TCAAGACCCA CATTGTTTCT ATTCCTCTCC GCGGCTGTGA TGTGATTCCG
    GGCTTGGACT CCAGACACCC CCTGACGTTC CGACTGCTTC GCAATGGACA GGAGGTGGCC
    GTGCTAGAGG CCTCCTCTTC TGAGGACATG GGCAGATGGA TTGGGATTTT GCTGGCCGAG
    ACAGGATCCT CTACAGACCC TGGGGCCCTG CACTACGACT ATATCGACGT GGAGACATCT
    GCGAACGTCA TTCAGACAGC CAAACAGACA TTCTGTTTCA TGAACAGGCG CGGTATCTCT
    GCCAACCCGT ACCTGGGGAG CACCACCAAC GGCTACGCCC ACCCCAGTGG CATGGCGCTG
    CATTACGATG ACGTCCCATG CATTAACGGC TCGTGGGACC CCGAAGACGG CCCTCCTGCT
    TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG CTTTATGATA ACGCGGTCCT GTACGATAAC
    TTGCCGTCTC CGAAAATCTT TGCTCGCTAC CCACCTGCTG ACAGAAAGGC TTCTAGGCCG
    TCTCCTGACA AACTGTCCTG TAACCATTAC AAATGCCCTC CCTCCTGCGG TCTGCCTCTC
    ATTCAGTCTG CCACTAACCC CTCTTCTCTG GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG
    GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT AGTGGGGTTA CGGGGAAAGG GAAGACTCTG
    AGCAGCCAGC AAAAAAAGGT AGATTCCACA GCCGGTGTGA AGCGAACATC TTCAAATGCT
    GACCAGTACA AATATGGCAA GAATCGGGTT GAAGCCGATG CCAAGCGGTT GCAGAGCAAA
    GAAGATGAGC TGCTAAAGCG TAAAGAAACC CTGCGGAACA GGCTGGCCCA GCTCCGGAAA
    GAGAGGAGAG ACCTGAGGGC TGCCATCGAA GTAAACGCTG GCAGGAAGAC GCAGGTGGTT
    CTGGAAGACA AGCTGAAGAG GCTGGAGGAG GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC
    AACCTGGAGC TGGAGCTGAC GGAAGTCACA GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG
    GTCACCCTGG GGCTGGCCAT CGAGCCCAGG TCAGGGACAT CGAGCCCACA GTCCCCGGTT
    TTCAGGCATC GCACCCTGGA GAGCTCACCC ATGTCCAGCT GTGAGACCAG CGATGCGGAG
    GGCCCCGTGC CGGTGAACAG TGCAGCCGTG CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC
    CCCTGCCGGG GACATGTGCT GCGGAAGGCC AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967982.1
    CDS472..2991
    Translation

    Target ORF information:

    RefSeq Version XM_014112507.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTGA TCCGAGATGT CTACAGTGGC TGCAGTGGGC CGGCGGATTC AGAAGGCCCA
    CCCCCATCAA GCTCCCCAGT GCACAAGACA GAGCTGGAGA AGAAACTGTC TTCTGAGAGA
    CCCAGCTCTG ATGGAGAGGG TGTTACAGAA AATGGCATCA CTGTGTGTAA TGGAAAAGAA
    CAAGTTAAGA GGAAAAAGAG TTCCAAATCA GAGGCCAAGG GCACAGTGTC TAAAGTCACT
    GGGAAGAAGA TCACCAAGAT CATTGGCTTG GGGAAGAAAA AGCCATCAAC AGATGAGCAG
    ACCTCATCAG CAGAGGAGGA TGTCCCCACT TGTGGCCTAT GGAAGAAGCC CCTGCCCCTT
    GATGAGGGCA CCCCATCCCT GAACCACTCG GGCACAGGCT ATCTCAACGT GCTCTCCAAC
    AACCGCTGGC GGGAGCGCTG GTGCCGGGTC AAGGATAACA AGCTCATTTT CCACAAGGAC
    AGGACTGATC TCAAGACCCA CATTGTTTCT ATTCCTCTCC GCGGCTGTGA TGTGATTCCG
    GGCTTGGACT CCAGACACCC CCTGACGTTC CGACTGCTTC GCAATGGACA GGAGGTGGCC
    GTGCTAGAGG CCTCCTCTTC TGAGGACATG GGCAGATGGA TTGGGATTTT GCTGGCCGAG
    ACAGGATCCT CTACAGACCC TGGGGCCCTG CACTACGACT ATATCGACGT GGAGACATCT
    GCGAACGTCA TTCAGACAGC CAAACAGACA TTCTGTTTCA TGAACAGGCG CGGTATCTCT
    GCCAACCCGT ACCTGGGGAG CACCACCAAC GGCTACGCCC ACCCCAGTGG CATGGCGCTG
    CATTACGATG ACGTCCCATG CATTAACGGC TCGTGGGACC CCGAAGACGG CCCTCCTGCT
    TCCTGTGCCA GAGGCCTGGG AGAAGAGGTG CTTTATGATA ACGCGGTCCT GTACGATAAC
    TTGCCGTCTC CGAAAATCTT TGCTCGCTAC CCACCTGCTG ACAGAAAGGC TTCTAGGCCG
    TCTCCTGACA AACTGTCCTG TAACCATTAC AAATGCCCTC CCTCCTGCGG TCTGCCTCTC
    ATTCAGTCTG CCACTAACCC CTCTTCTCTG GGGAGGGCGT CTCTCGGGCT CAGCTCTCAG
    GTGAAGGGTA AAAAGCCCCC AGTAGCCTCT AGTGGGGTTA CGGGGAAAGG GAAGACTCTG
    AGCAGCCAGC AAAAAAAGGT AGATTCCACA GCCGGTGTGA AGCGAACATC TTCAAATGCT
    GACCAGTACA AATATGGCAA GAATCGGGTT GAAGCCGATG CCAAGCGGTT GCAGAGCAAA
    GAAGATGAGC TGCTAAAGCG TAAAGAAACC CTGCGGAACA GGCTGGCCCA GCTCCGGAAA
    GAGAGGAGAG ACCTGAGGGC TGCCATCGAA GTAAACGCTG GCAGGAAGAC GCAGGTGGTT
    CTGGAAGACA AGCTGAAGAG GCTGGAGGAG GAGTGTAAGC AGAAGGAGGC GGAGCGTGTC
    AACCTGGAGC TGGAGCTGAC GGAAGTCACA GAGAGCCTGA AGAAGGCCCT GGCTGGCGGG
    GTCACCCTGG GGCTGGCCAT CGAGCCCAGG TCAGGGACAT CGAGCCCACA GTCCCCGGTT
    TTCAGGCATC GCACCCTGGA GAGCTCACCC ATGTCCAGCT GTGAGACCAG CGATGCGGAG
    GGCCCCGTGC CGGTGAACAG TGCAGCCGTG CTGAGGAAGG GCCAGCCGGC CAGCGGCTCC
    CCCTGCCGGG GACATGTGCT GCGGAAGGCC AAGGAATGGG AGCTGAAGAA CGGCACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35772
    Clone ID Related Accession (Same CDS sequence) XM_014112508.1
    Accession Version XM_014112508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product actin filament-associated protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCTATC TCAACGTGCT CTCCAACAAC CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG
    GATAACAAGC TCATTTTCCA CAAGGACAGG ACTGATCTCA AGACCCACAT TGTTTCTATT
    CCTCTCCGCG GCTGTGATGT GATTCCGGGC TTGGACTCCA GACACCCCCT GACGTTCCGA
    CTGCTTCGCA ATGGACAGGA GGTGGCCGTG CTAGAGGCCT CCTCTTCTGA GGACATGGGC
    AGATGGATTG GGATTTTGCT GGCCGAGACA GGATCCTCTA CAGACCCTGG GGCCCTGCAC
    TACGACTATA TCGACGTGGA GACATCTGCG AACGTCATTC AGACAGCCAA ACAGACATTC
    TGTTTCATGA ACAGGCGCGG TATCTCTGCC AACCCGTACC TGGGGAGCAC CACCAACGGC
    TACGCCCACC CCAGTGGCAT GGCGCTGCAT TACGATGACG TCCCATGCAT TAACGGCTCG
    TGGGACCCCG AAGACGGCCC TCCTGCTTCC TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT
    TATGATAACG CGGTCCTGTA CGATAACTTG CCGTCTCCGA AAATCTTTGC TCGCTACCCA
    CCTGCTGACA GAAAGGCTTC TAGGCCGTCT CCTGACAAAC TGTCCTGTAA CCATTACAAA
    TGCCCTCCCT CCTGCGGTCT GCCTCTCATT CAGTCTGCCA CTAACCCCTC TTCTCTGGGG
    AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT
    GGGGTTACGG GGAAAGGGAA GACTCTGAGC AGCCAGCAAA AAAAGGTAGA TTCCACAGCC
    GGTGTGAAGC GAACATCTTC AAATGCTGAC CAGTACAAAT ATGGCAAGAA TCGGGTTGAA
    GCCGATGCCA AGCGGTTGCA GAGCAAAGAA GATGAGCTGC TAAAGCGTAA AGAAACCCTG
    CGGAACAGGC TGGCCCAGCT CCGGAAAGAG AGGAGAGACC TGAGGGCTGC CATCGAAGTA
    AACGCTGGCA GGAAGACGCA GGTGGTTCTG GAAGACAAGC TGAAGAGGCT GGAGGAGGAG
    TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC CTGGAGCTGG AGCTGACGGA AGTCACAGAG
    AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC ACCCTGGGGC TGGCCATCGA GCCCAGGTCA
    GGGACATCGA GCCCACAGTC CCCGGTTTTC AGGCATCGCA CCCTGGAGAG CTCACCCATG
    TCCAGCTGTG AGACCAGCGA TGCGGAGGGC CCCGTGCCGG TGAACAGTGC AGCCGTGCTG
    AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967983.1
    CDS473..2959
    Translation

    Target ORF information:

    RefSeq Version XM_014112508.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris actin filament associated protein 1 (AFAP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAGAGT TAATAGTTGA ACTCCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACATCAACTG TTAGAGAGAA AAAGGCAGTT ATCACCGACA TCCTGTTGAG GATACAGTCA
    TCTAAAGGTT TCGAAGTGAA GGATCATGCT CAGAAGCAAG AGACCCCCAA TAGCCTGCCG
    GCTCCTCCCC AGATGCCTTT GCCTGAGATC CCCCAGCCAT GGCTGCCACC TGACAGTGGG
    CCGCCACCGC TACCAACATC CTCTCTCCCG GAAGGTTACT ACGAGGAAGC TGTACCACTG
    AGCCCTGGGA AAGCTCCGGA ATACATCACG TCAAATTATG ACTCAGATGC AATGAGTAGC
    TCTTATGAGT CTTATGATGA GGAGGAAGAG GATGGAAAGG GCAAGAAGAC ACGGCACCAG
    TGGCCGTCTG AGGAGGCCTC CATGGACCTG GTGAAGGATG CCAAAATCTG TGCCTTCCTA
    CTGAGGAAGA AGCGCTTCGG GCAGTGGAGC AAGCTTCTTT GTGTCGTCAA AGACACCAAA
    CTGCTGTGCT ACAAAAGTTC CAAGGACCAA CAGCCTCAGA TGGAACTGCC GCTGCAGGGC
    TGCAACGTGA CGTACACACC GAAGGACAGT AAGAAGAAGA AACACGAGCT GAAAATCACC
    CAGCAGGGCA CAGACCCACT GGTCCTCGCC GTTCAGAGCA AGGAGCAGGC TGAGCAGTGG
    CTGAAGGTAA CTTCTATCCT GAATATTATC ACCAAGGTGA TCCGAGATGT CTACAGTGGC
    TGCAGTGGGC CGGCGGATTC AGAAGGCCCA CCCCCATCAA GCTCCCCAGT GCACAAGACA
    GAGCTGGAGA AGAAACTGTC TTCTGAGAGA CCCAGCTCTG ATGGAGAGGG TGTTACAGAA
    AATGGCATCA CTGTGTGTAA TGGAAAAGAA CAAGTTAAGA GGAAAAAGAG TTCCAAATCA
    GAGGCCAAGG GCACAGTGTC TAAAGTCACT GGGAAGAAGA TCACCAAGAT CATTGGCTTG
    GGGAAGAAAA AGCCATCAAC AGATGAGCAG ACCTCATCAG CAGAGGAGGA TGTCCCCACT
    TGTGGCTATC TCAACGTGCT CTCCAACAAC CGCTGGCGGG AGCGCTGGTG CCGGGTCAAG
    GATAACAAGC TCATTTTCCA CAAGGACAGG ACTGATCTCA AGACCCACAT TGTTTCTATT
    CCTCTCCGCG GCTGTGATGT GATTCCGGGC TTGGACTCCA GACACCCCCT GACGTTCCGA
    CTGCTTCGCA ATGGACAGGA GGTGGCCGTG CTAGAGGCCT CCTCTTCTGA GGACATGGGC
    AGATGGATTG GGATTTTGCT GGCCGAGACA GGATCCTCTA CAGACCCTGG GGCCCTGCAC
    TACGACTATA TCGACGTGGA GACATCTGCG AACGTCATTC AGACAGCCAA ACAGACATTC
    TGTTTCATGA ACAGGCGCGG TATCTCTGCC AACCCGTACC TGGGGAGCAC CACCAACGGC
    TACGCCCACC CCAGTGGCAT GGCGCTGCAT TACGATGACG TCCCATGCAT TAACGGCTCG
    TGGGACCCCG AAGACGGCCC TCCTGCTTCC TGTGCCAGAG GCCTGGGAGA AGAGGTGCTT
    TATGATAACG CGGTCCTGTA CGATAACTTG CCGTCTCCGA AAATCTTTGC TCGCTACCCA
    CCTGCTGACA GAAAGGCTTC TAGGCCGTCT CCTGACAAAC TGTCCTGTAA CCATTACAAA
    TGCCCTCCCT CCTGCGGTCT GCCTCTCATT CAGTCTGCCA CTAACCCCTC TTCTCTGGGG
    AGGGCGTCTC TCGGGCTCAG CTCTCAGGTG AAGGGTAAAA AGCCCCCAGT AGCCTCTAGT
    GGGGTTACGG GGAAAGGGAA GACTCTGAGC AGCCAGCAAA AAAAGGTAGA TTCCACAGCC
    GGTGTGAAGC GAACATCTTC AAATGCTGAC CAGTACAAAT ATGGCAAGAA TCGGGTTGAA
    GCCGATGCCA AGCGGTTGCA GAGCAAAGAA GATGAGCTGC TAAAGCGTAA AGAAACCCTG
    CGGAACAGGC TGGCCCAGCT CCGGAAAGAG AGGAGAGACC TGAGGGCTGC CATCGAAGTA
    AACGCTGGCA GGAAGACGCA GGTGGTTCTG GAAGACAAGC TGAAGAGGCT GGAGGAGGAG
    TGTAAGCAGA AGGAGGCGGA GCGTGTCAAC CTGGAGCTGG AGCTGACGGA AGTCACAGAG
    AGCCTGAAGA AGGCCCTGGC TGGCGGGGTC ACCCTGGGGC TGGCCATCGA GCCCAGGTCA
    GGGACATCGA GCCCACAGTC CCCGGTTTTC AGGCATCGCA CCCTGGAGAG CTCACCCATG
    TCCAGCTGTG AGACCAGCGA TGCGGAGGGC CCCGTGCCGG TGAACAGTGC AGCCGTGCTG
    AGGAAGGGCC AGCCGGCCAG CGGCTCCCCC TGCCGGGGAC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAGC TGAAGAACGG CACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.