afap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following afap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the afap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa04798 XM_004911244.3
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa04799 XM_002938257.4
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa04800 XM_004911245.3
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa04801 XM_004911246.3
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa04802 XM_004911247.3
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa49623 XM_004911244.4
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa49624 XM_002938257.5
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa49625 XM_004911245.4
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa49626 XM_004911246.4
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa49627 XM_004911247.4
Latest version!
Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa04798
    Clone ID Related Accession (Same CDS sequence) XM_004911244.3
    Accession Version XM_004911244.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGGGAAGTC AATGTGATAA TGAAATCCTC TTGGTTTTGC AGTCACCGGT TCTAAAGCAC
    CGCACGGTGG AGAATTCTCC CATCTCCAGC TGTGATACAA GCGATGCCGA ATGCTCCCTA
    CCTGTGAACA GTGCAGCCGC TTTAAAGAGA CAGCCCTCTG CAAGTAACTC ACCTGTACGG
    GGACATGTTT TGAGGAAGGC CAAGGAATGG GAACTTAAAA ATGGCACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911301.1
    CDS171..2681
    Translation

    Target ORF information:

    RefSeq Version XM_004911244.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911244.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGGGAAGTC AATGTGATAA TGAAATCCTC TTGGTTTTGC AGTCACCGGT TCTAAAGCAC
    CGCACGGTGG AGAATTCTCC CATCTCCAGC TGTGATACAA GCGATGCCGA ATGCTCCCTA
    CCTGTGAACA GTGCAGCCGC TTTAAAGAGA CAGCCCTCTG CAAGTAACTC ACCTGTACGG
    GGACATGTTT TGAGGAAGGC CAAGGAATGG GAACTTAAAA ATGGCACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04799
    Clone ID Related Accession (Same CDS sequence) XM_002938257.4
    Accession Version XM_002938257.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002938257.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGTCACCGG TTCTAAAGCA CCGCACGGTG GAGAATTCTC CCATCTCCAG CTGTGATACA
    AGCGATGCCG AATGCTCCCT ACCTGTGAAC AGTGCAGCCG CTTTAAAGAG ACAGCCCTCT
    GCAAGTAACT CACCTGTACG GGGACATGTT TTGAGGAAGG CCAAGGAATG GGAACTTAAA
    AATGGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938303.2
    CDS169..2640
    Translation

    Target ORF information:

    RefSeq Version XM_002938257.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938257.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGTCACCGG TTCTAAAGCA CCGCACGGTG GAGAATTCTC CCATCTCCAG CTGTGATACA
    AGCGATGCCG AATGCTCCCT ACCTGTGAAC AGTGCAGCCG CTTTAAAGAG ACAGCCCTCT
    GCAAGTAACT CACCTGTACG GGGACATGTT TTGAGGAAGG CCAAGGAATG GGAACTTAAA
    AATGGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04800
    Clone ID Related Accession (Same CDS sequence) XM_004911245.3
    Accession Version XM_004911245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGCACCGCA CGGTGGAGAA TTCTCCCATC TCCAGCTGTG ATACAAGCGA TGCCGAATGC
    TCCCTACCTG TGAACAGTGC AGCCGCTTTA AAGAGACAGC CCTCTGCAAG TAACTCACCT
    GTACGGGGAC ATGTTTTGAG GAAGGCCAAG GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911302.1
    CDS170..2626
    Translation

    Target ORF information:

    RefSeq Version XM_004911245.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGCACCGCA CGGTGGAGAA TTCTCCCATC TCCAGCTGTG ATACAAGCGA TGCCGAATGC
    TCCCTACCTG TGAACAGTGC AGCCGCTTTA AAGAGACAGC CCTCTGCAAG TAACTCACCT
    GTACGGGGAC ATGTTTTGAG GAAGGCCAAG GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04801
    Clone ID Related Accession (Same CDS sequence) XM_004911246.3
    Accession Version XM_004911246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGG GAAGTCAATG TGATAATGAA
    ATCCTCTTGG TTTTGCAGTC ACCGGTTCTA AAGCACCGCA CGGTGGAGAA TTCTCCCATC
    TCCAGCTGTG ATACAAGCGA TGCCGAATGC TCCCTACCTG TGAACAGTGC AGCCGCTTTA
    AAGAGACAGC CCTCTGCAAG TAACTCACCT GTACGGGGAC ATGTTTTGAG GAAGGCCAAG
    GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911303.1
    CDS169..2415
    Translation

    Target ORF information:

    RefSeq Version XM_004911246.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGG GAAGTCAATG TGATAATGAA
    ATCCTCTTGG TTTTGCAGTC ACCGGTTCTA AAGCACCGCA CGGTGGAGAA TTCTCCCATC
    TCCAGCTGTG ATACAAGCGA TGCCGAATGC TCCCTACCTG TGAACAGTGC AGCCGCTTTA
    AAGAGACAGC CCTCTGCAAG TAACTCACCT GTACGGGGAC ATGTTTTGAG GAAGGCCAAG
    GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04802
    Clone ID Related Accession (Same CDS sequence) XM_004911247.3
    Accession Version XM_004911247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGT CACCGGTTCT AAAGCACCGC
    ACGGTGGAGA ATTCTCCCAT CTCCAGCTGT GATACAAGCG ATGCCGAATG CTCCCTACCT
    GTGAACAGTG CAGCCGCTTT AAAGAGACAG CCCTCTGCAA GTAACTCACC TGTACGGGGA
    CATGTTTTGA GGAAGGCCAA GGAATGGGAA CTTAAAAATG GCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911304.1
    CDS167..2374
    Translation

    Target ORF information:

    RefSeq Version XM_004911247.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGCTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGT CACCGGTTCT AAAGCACCGC
    ACGGTGGAGA ATTCTCCCAT CTCCAGCTGT GATACAAGCG ATGCCGAATG CTCCCTACCT
    GTGAACAGTG CAGCCGCTTT AAAGAGACAG CCCTCTGCAA GTAACTCACC TGTACGGGGA
    CATGTTTTGA GGAAGGCCAA GGAATGGGAA CTTAAAAATG GCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49623
    Clone ID Related Accession (Same CDS sequence) XM_004911244.4
    Accession Version XM_004911244.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911244.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGGGAAGTC AATGTGATAA TGAAATCCTC TTGGTTTTGC AGTCACCGGT TCTAAAGCAC
    CGCACGGTGG AGAATTCTCC CATCTCCAGC TGTGATACAA GCGATGCCGA ATGCTCCCTA
    CCTGTGAACA GTGCAGCCGC TTTAAAGAGA CAGCCCTCTG CAAGTAACTC ACCTGTACGG
    GGACATGTTT TGAGGAAGGC CAAGGAATGG GAACTTAAAA ATGGCACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911301.1
    CDS153..2663
    Translation

    Target ORF information:

    RefSeq Version XM_004911244.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911244.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGGGAAGTC AATGTGATAA TGAAATCCTC TTGGTTTTGC AGTCACCGGT TCTAAAGCAC
    CGCACGGTGG AGAATTCTCC CATCTCCAGC TGTGATACAA GCGATGCCGA ATGCTCCCTA
    CCTGTGAACA GTGCAGCCGC TTTAAAGAGA CAGCCCTCTG CAAGTAACTC ACCTGTACGG
    GGACATGTTT TGAGGAAGGC CAAGGAATGG GAACTTAAAA ATGGCACATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49624
    Clone ID Related Accession (Same CDS sequence) XM_002938257.5
    Accession Version XM_002938257.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002938257.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGTCACCGG TTCTAAAGCA CCGCACGGTG GAGAATTCTC CCATCTCCAG CTGTGATACA
    AGCGATGCCG AATGCTCCCT ACCTGTGAAC AGTGCAGCCG CTTTAAAGAG ACAGCCCTCT
    GCAAGTAACT CACCTGTACG GGGACATGTT TTGAGGAAGG CCAAGGAATG GGAACTTAAA
    AATGGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938303.2
    CDS151..2622
    Translation

    Target ORF information:

    RefSeq Version XM_002938257.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938257.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGTCACCGG TTCTAAAGCA CCGCACGGTG GAGAATTCTC CCATCTCCAG CTGTGATACA
    AGCGATGCCG AATGCTCCCT ACCTGTGAAC AGTGCAGCCG CTTTAAAGAG ACAGCCCTCT
    GCAAGTAACT CACCTGTACG GGGACATGTT TTGAGGAAGG CCAAGGAATG GGAACTTAAA
    AATGGCACAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49625
    Clone ID Related Accession (Same CDS sequence) XM_004911245.4
    Accession Version XM_004911245.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911245.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGCACCGCA CGGTGGAGAA TTCTCCCATC TCCAGCTGTG ATACAAGCGA TGCCGAATGC
    TCCCTACCTG TGAACAGTGC AGCCGCTTTA AAGAGACAGC CCTCTGCAAG TAACTCACCT
    GTACGGGGAC ATGTTTTGAG GAAGGCCAAG GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911302.1
    CDS152..2608
    Translation

    Target ORF information:

    RefSeq Version XM_004911245.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911245.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGTG GAGACCCGAT
    GAGGACTATG AGACTTACCG TAACAGAAAG AATGGAGAAG AAGTACATTA CGATGATGCA
    GACCTGTATG ATAACTTGCC ATCTCCGAAA ATCTTTGCCC GCTATCCACC AGCCGATAAA
    AAAGCCAATA GGTTTTCTAC TGATAAACTC CCTCCTAACC ACTACAAATC TCCTGTCTCA
    TCCAGTCTGT CTTCTCAGTC TGTCACTAAC AACTCTGCTG TAGGGAGGGG ACCTCTCAGT
    TCCCAGTCTC AGCTGAAAAG CAAGAAGCCA CCTGCCATGG CCAACGGAAT TGCTGCAAAA
    GGAAGAATAA ACAACAGCCA GCCGAAAAAT GCAGCTCCAG CAGCCAACGT GAAACGCACC
    GCCTCAAATG CCGAGCAGTA CAAATATGGG AAGAACCGGG TGGAAGCAGA TGCGAAAAAA
    CTGCAGATGA AAGAGGAGGA ATTGCTGAGA AAGAAAGAAG CGCTACGGAA CAGACTGGCT
    CAGCTTCGGA AGGAGAAAAG GGACATACGA GCTGCCATCG AAGCCAATGC TGGGAGGAAA
    TCCCAGGTGC TGCTGGAAGA GAAGATGAAG AAGCTGGAGG ATGAATGCAA ACAGAAAGAG
    CGTGAGCGGG TTACCCTGGA GCTGGAGCTT ACTGAGGTGA AGGAGAGTCT GAAGAAAGCC
    ATGGCTGGGG GCATCACGCT CGGCCTCGCA ATAGAACCCA AGCAGGGATC ATCTCGGTCC
    CAGCACCGCA CGGTGGAGAA TTCTCCCATC TCCAGCTGTG ATACAAGCGA TGCCGAATGC
    TCCCTACCTG TGAACAGTGC AGCCGCTTTA AAGAGACAGC CCTCTGCAAG TAACTCACCT
    GTACGGGGAC ATGTTTTGAG GAAGGCCAAG GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49626
    Clone ID Related Accession (Same CDS sequence) XM_004911246.4
    Accession Version XM_004911246.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911246.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGG GAAGTCAATG TGATAATGAA
    ATCCTCTTGG TTTTGCAGTC ACCGGTTCTA AAGCACCGCA CGGTGGAGAA TTCTCCCATC
    TCCAGCTGTG ATACAAGCGA TGCCGAATGC TCCCTACCTG TGAACAGTGC AGCCGCTTTA
    AAGAGACAGC CCTCTGCAAG TAACTCACCT GTACGGGGAC ATGTTTTGAG GAAGGCCAAG
    GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911303.1
    CDS151..2397
    Translation

    Target ORF information:

    RefSeq Version XM_004911246.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911246.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGG GAAGTCAATG TGATAATGAA
    ATCCTCTTGG TTTTGCAGTC ACCGGTTCTA AAGCACCGCA CGGTGGAGAA TTCTCCCATC
    TCCAGCTGTG ATACAAGCGA TGCCGAATGC TCCCTACCTG TGAACAGTGC AGCCGCTTTA
    AAGAGACAGC CCTCTGCAAG TAACTCACCT GTACGGGGAC ATGTTTTGAG GAAGGCCAAG
    GAATGGGAAC TTAAAAATGG CACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49627
    Clone ID Related Accession (Same CDS sequence) XM_004911247.4
    Accession Version XM_004911247.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product actin filament-associated protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911247.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGT CACCGGTTCT AAAGCACCGC
    ACGGTGGAGA ATTCTCCCAT CTCCAGCTGT GATACAAGCG ATGCCGAATG CTCCCTACCT
    GTGAACAGTG CAGCCGCTTT AAAGAGACAG CCCTCTGCAA GTAACTCACC TGTACGGGGA
    CATGTTTTGA GGAAGGCCAA GGAATGGGAA CTTAAAAATG GCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004911304.1
    CDS149..2356
    Translation

    Target ORF information:

    RefSeq Version XM_004911247.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis actin filament associated protein 1 (afap1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004911247.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCAGAAGT ACTGCAAAGA AATGGAGGAA CTCATAGTGG AACTGCGGCT GTTTCTCGAC 
    TTGTTGGACC ATGAATTTCT CACAACAACA GTCAGAGAGA AAAAGACTTT AATAAGCAAC
    ATCCTGTACA GGATTCAGTC GGCCAGAGAA TCTGAACTGA AGAACCATAA CCACAGAGCC
    GAGATGCCCT GCAGTGTGCC TGCCCCACCT CAGATGCCTC TGCCAGAAAT CCCTCAGCAG
    TGGTTGCCTC CAGATAATGG ACCTCCTCCC CTTCCAACAT CCTCCCTTCC CGAGGGCTAC
    TATGAGGAAG CGGTACCGCT CAGCCCTGGA AAGGCTCCAG AGTACATCAC TTCCAACTAT
    GACTCGGATG CCATGAGTAG CTCATATGAA TCCTATGATG AGGAGGAAGA GGATGGTAAA
    GGGCAGAAGA AAAGGCTTCA GTGGCCTTCC GAAGAAGCAT CTATGGATCT TGTAAAGGAT
    GCCAAAATTT GTGCTTTCCT TCTACGGAAG AAAAGATTCG GCCAGTGGAC CAAACTGCTG
    TGTGTCATCA AAGACAACAA ATTAATGTGC TACAAAACCT CTAAGGACCA GCAACCGCAG
    ATGGAGCTGC CGCTGAACGG CTGTGCGGTC ACTTATGTAC CAAAAGACAG TAAGAAGAAG
    AAGCACGAGC TGAAGGTTAC TCACCAAGGG ACCGATGCCT TAGTTCTTGC AGTGCAGAGC
    GAGGAACAGG CCGAGCAGTG GCTGAGGGTA ATCAAAGATG CTTGCAATAT TTCTGGTGGA
    CCCATTGATT CCGATGGACC AATATCGAGC TCCCCTGTGC CAAAAAGTGA GCTGGAAAAG
    AAATTGTCGA TAGAGCGGCC GAGTTCGGAT GGAGAGGGAA ATGTAGAGAA TGGCACAGTG
    ATAACTAACG ACAAGGAGCC AGTGAAAAGG AAAAAGAGTG GCAAAGTCGA ACCTAAAGGC
    ACAGTGACAA AAGTAACTGG AAAAAAAATA ACAAAAATAA TTGGTCTGGG AAAGAAAAAG
    CCAAGTACCG ATGAGCAGAC GTCGTCAGCA GAAGAAGATG TCCCAACATG TGGATATCTC
    AATGTCCTGT CCAACAACCG CTGGAGGGAA CGCTGGTGCA GAGTCAAAGA CAACAAACTC
    ATTTTCCACA AGGACAGGAC AGATCTGAAG ACCCACATTG TTTCAATCCC CCTTAGAGGG
    TGTGAAGTTA TTCCTGGATT GGATTCCAAA CACCCCTTGA CATTCCGGCT GCTCCGTAAC
    GGACAAGAAG TCGCCGTGCT GGAGGCCTCA TCCTCTGAAA AAATGGGAAG GTGGCTTGGA
    ATATTACTGG CCGAGACTGG TTCCTCCACA GATCCTGGGG CTCTGCACTA TGATTACATT
    GATGTGGATA CAACTGCAAA TGTGATACAG ACTGCAAAGC AGTCTTTCTG CTTTATGAAC
    AGAAGAGTAA TCTCTGCAAA CCCATACCGA GAAGCTACTA CAAATGGCTA CTCTTGTCCC
    AGTGGAGTGG CCCTTCATTA CGATGATGTT CCCTGCATTA ATGGATTGCT GAAAAGCAAG
    AAGCCACCTG CCATGGCCAA CGGAATTGCT GCAAAAGGAA GAATAAACAA CAGCCAGCCG
    AAAAATGCAG CTCCAGCAGC CAACGTGAAA CGCACCGCCT CAAATGCCGA GCAGTACAAA
    TATGGGAAGA ACCGGGTGGA AGCAGATGCG AAAAAACTGC AGATGAAAGA GGAGGAATTG
    CTGAGAAAGA AAGAAGCGCT ACGGAACAGA CTGGCTCAGC TTCGGAAGGA GAAAAGGGAC
    ATACGAGCTG CCATCGAAGC CAATGCTGGG AGGAAATCCC AGGTGCTGCT GGAAGAGAAG
    ATGAAGAAGC TGGAGGATGA ATGCAAACAG AAAGAGCGTG AGCGGGTTAC CCTGGAGCTG
    GAGCTTACTG AGGTGAAGGA GAGTCTGAAG AAAGCCATGG CTGGGGGCAT CACGCTCGGC
    CTCGCAATAG AACCCAAGCA GGGATCATCT CGGTCCCAGT CACCGGTTCT AAAGCACCGC
    ACGGTGGAGA ATTCTCCCAT CTCCAGCTGT GATACAAGCG ATGCCGAATG CTCCCTACCT
    GTGAACAGTG CAGCCGCTTT AAAGAGACAG CCCTCTGCAA GTAACTCACC TGTACGGGGA
    CATGTTTTGA GGAAGGCCAA GGAATGGGAA CTTAAAAATG GCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.