gRNAs
The following AFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa28046 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204205.1 | |
| Accession Version | NM_204205.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Gallus gallus(chicken) | |
| Product | actin filament-associated protein 1 isoform a | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L20302.2. Transcript Variant: This variant (a) represents the longer transcript and encodes the longer isoform (a), also known as AFAP-120. ##Evidence-Data-START## Transcript exon combination :: L20302.2 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGGAAGAGT TAATAGTTGA GCTGCGACTG TTTCTTGAAC TTCTGGACCA TGAATATCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_989536.1 |
| CDS | 19..2466 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_204205.1 |
1 | ATGGAAGAGT TAATAGTTGA GCTGCGACTG TTTCTTGAAC TTCTGGACCA TGAATATCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa28138 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001134648.1 | |
| Accession Version | NM_001134648.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Gallus gallus(chicken) | |
| Product | actin filament-associated protein 1 isoform b | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L20303.2. Transcript Variant: This variant (b) lacks an alternate in-frame exon, compared to variant a. The resulting protein (isoform b), also known as AFAP-110, lacks an internal protein-binding domain, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: L20303.2 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGGAAGAGT TAATAGTTGA GCTGCGACTG TTTCTTGAAC TTCTGGACCA TGAATATCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001128120.1 |
| CDS | 19..2208 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001134648.1 |
1 | ATGGAAGAGT TAATAGTTGA GCTGCGACTG TTTCTTGAAC TTCTGGACCA TGAATATCTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The actin-binding domain of actin filament-associated protein (AFAP) is involved in the regulation of cytoskeletal structure. |
Actin filament associated protein mediates c-Src related SRE/AP-1 transcriptional activation. |
Conversion of mechanical force into biochemical signaling. |
Stabilization and remodeling of the membrane skeleton during lens fiber cell differentiation and maturation. |
Formation of a stable src-AFAP-110 complex through either an amino-terminal or a carboxy-terminal SH2-binding motif. |
