AFAP1 cDNA ORF clone, Homo sapiens(human)

The following AFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25963 NM_001371090.1
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25963 NM_001371091.1
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu25963 NM_198595.2
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu26145 NM_001134647.1
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu26145 XM_011513544.3
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu38377 XM_006713909.3
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu95369 XM_017008535.1
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu95370 XM_017008536.1
Latest version!
Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu26145 XM_011513544.2 Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25963
    Clone ID Related Accession (Same CDS sequence) NM_001371090.1 , NM_001371091.1 , NM_198595.2
    Accession Version NM_001371090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC144451.3, AC141931.2, AC112254.6 and AC004169.3. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 2, 3 and 4 encodes the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358019.1
    CDS231..2423
    Translation

    Target ORF information:

    RefSeq Version NM_001371090.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25963
    Clone ID Related Accession (Same CDS sequence) NM_001371090.1 , NM_001371091.1 , NM_198595.2
    Accession Version NM_001371091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC097381.3, AC144451.3, AC141931.2, AC112254.6 and AC004169.3. Transcript Variant: This variant (4) represents the longest transcript. Variants 2, 3 and 4 encodes the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001358020.1
    CDS987..3179
    Translation

    Target ORF information:

    RefSeq Version NM_001371091.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001371091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25963
    Clone ID Related Accession (Same CDS sequence) NM_001371090.1 , NM_001371091.1 , NM_198595.2
    Accession Version NM_198595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB450895.1, DA605903.1, BC032777.1, AF188700.1, AC004169.3 and CA448961.1. On Feb 14, 2007 this sequence version replaced NM_198595.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 4. Variants 2, 3 and 4 encodes the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.213062.1, SRR1803617.28090.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_940997.1
    CDS274..2466
    Translation

    Target ORF information:

    RefSeq Version NM_198595.2
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_198595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG CTCAAGGGTA AAAAGCCCCC CGTGGCGTCT
    AATGGGGTCA CAGGAAAAGG GAAGACTCTG AGCAGTCAGC CAAAGAAAGC GGATCCCGCG
    GCTGTTGTGA AAAGGACGGG TTCGAATGCT GCCCAGTACA AGTATGGCAA GAACCGGGTA
    GAAGCAGATG CCAAGCGGCT ACAGACCAAA GAGGAGGAGC TGCTGAAGAG GAAAGAGGCC
    CTGCGGAATA GGCTGGCCCA GCTCCGCAAG GAAAGAAAAG ACCTTCGAGC GGCTATTGAA
    GTGAACGCCG GCAGGAAGCC GCAGGCGATC CTGGAGGAGA AGCTGAAGCA GCTGGAGGAG
    GAGTGCCGGC AGAAGGAGGC GGAGCGTGTC AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG
    GAGAGCCTGA AGAAAGCGCT GGCGGGCGGA GTCACCCTGG GGCTGGCCAT CGAGCCCAAG
    TCAGGGACAT CGAGTCCACA GTCTCCAGTG TTCCGGCACC GGACCCTGGA AAACTCGCCC
    ATCTCCAGCT GTGACACCAG TGACACCGAG GGCCCCGTGC CGGTGAACAG CGCGGCCGTC
    TTGAAGAAGA GCCAGGCTGC CCCGGGCAGC TCCCCCTGCC GAGGGCATGT GCTGCGGAAG
    GCCAAGGAAT GGGAATTGAA GAACGGGACC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26145
    Clone ID Related Accession (Same CDS sequence) XM_011513544.3 , XM_011513544.2 , NM_001134647.1
    Accession Version NM_001134647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB450895.1, BI561136.1, AK290807.1, AK297631.1, AK127962.1, AC112254.6, AC004169.3 and CA448961.1. Transcript Variant: This variant (A) differs in the 5' UTR and includes an alternate in-frame exon in the coding region compared to variant 4. The variant encodes a longer protein, isoform A, compared to isoform B. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.124707.1, SRR1803613.111787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001128119.1
    CDS274..2718
    Translation

    Target ORF information:

    RefSeq Version NM_001134647.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001134647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26145
    Clone ID Related Accession (Same CDS sequence) XM_011513544.3 , XM_011513544.2 , NM_001134647.1
    Accession Version XM_011513544.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011513544.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511846.1
    CDS328..2772
    Translation

    Target ORF information:

    RefSeq Version XM_011513544.3
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513544.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26145
    Clone ID Related Accession (Same CDS sequence) XM_011513544.3 , XM_011513544.2 , NM_001134647.1
    Accession Version XM_011513544.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2445bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011511846.1
    CDS327..2771
    Translation

    Target ORF information:

    RefSeq Version XM_011513544.2
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011513544.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTGTA CGATAACTTG
    CCGCCTCCGC ACATCTTTGC CCGCTACTCT CCTGCTGACA GAAAGGCCTC TAGGCTGTCT
    GCTGACAAGC TGTCCTCTAA CCATTACAAA TACCCTGCCT CCGCTCAGTC TGTCACTAAT
    ACCTCTTCTG TGGGGAGGGC GTCTCTCGGG CTCAACTCGC AGCTCAAGGG TAAAAAGCCC
    CCCGTGGCGT CTAATGGGGT CACAGGAAAA GGGAAGACTC TGAGCAGTCA GCCAAAGAAA
    GCGGATCCCG CGGCTGTTGT GAAAAGGACG GGTTCGAATG CTGCCCAGTA CAAGTATGGC
    AAGAACCGGG TAGAAGCAGA TGCCAAGCGG CTACAGACCA AAGAGGAGGA GCTGCTGAAG
    AGGAAAGAGG CCCTGCGGAA TAGGCTGGCC CAGCTCCGCA AGGAAAGAAA AGACCTTCGA
    GCGGCTATTG AAGTGAACGC CGGCAGGAAG CCGCAGGCGA TCCTGGAGGA GAAGCTGAAG
    CAGCTGGAGG AGGAGTGCCG GCAGAAGGAG GCGGAGCGTG TCAGCCTGGA GCTGGAGCTG
    ACGGAGGTCA AGGAGAGCCT GAAGAAAGCG CTGGCGGGCG GAGTCACCCT GGGGCTGGCC
    ATCGAGCCCA AGTCAGGGAC ATCGAGTCCA CAGTCTCCAG TGTTCCGGCA CCGGACCCTG
    GAAAACTCGC CCATCTCCAG CTGTGACACC AGTGACACCG AGGGCCCCGT GCCGGTGAAC
    AGCGCGGCCG TCTTGAAGAA GAGCCAGGCT GCCCCGGGCA GCTCCCCCTG CCGAGGGCAT
    GTGCTGCGGA AGGCCAAGGA ATGGGAATTG AAGAACGGGA CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu38377
    Clone ID Related Accession (Same CDS sequence) XM_006713909.3
    Accession Version XM_006713909.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCGTTTCA AGCAGGAGCA TCCACACCCA CGGAATGAGG CCAAGGCAGT TACAGTTAGA 
    GCAATGGAAG AGTTAATAGT TGAACTTCGT CTCTTTCTTG AACTCCTGGA CCATGAATAT
    CTAACCTCAA CTGTCAGGGA GAAAAAGGCA GTGATAACCA ACATTCTGCT AAGAATACAG
    TCATCCAAAG GTTTTGATGT GAAGGACCAT GCTCAGAAGC AGGAGACCGC TAACAGCCTG
    CCAGCCCCTC CTCAGATGCC CCTGCCGGAG ATCCCTCAGC CCTGGCTGCC TCCTGACAGT
    GGGCCTCCAC CATTGCCAAC ATCCTCCCTC CCAGAAGGTT ATTATGAGGA AGCTGTGCCG
    CTGAGCCCCG GAAAAGCTCC GGAATACATC ACATCAAATT ATGATTCCGA TGCGATGAGC
    AGCTCTTATG AGTCGTATGA TGAAGAGGAG GAGGATGGGA AGGGGAAGAA AACCCGGCAC
    CAGTGGCCCT CCGAGGAGGC CTCCATGGAC CTGGTCAAGG ACGCCAAAAT CTGCGCCTTC
    CTGCTGCGGA AGAAGCGGTT CGGCCAGTGG ACCAAGTTGC TCTGCGTCAT CAAAGACACC
    AAACTGCTGT GCTATAAAAG TTCCAAGGAC CAGCAGCCTC AGATGGAACT GCCACTCCAA
    GGCTGTAACA TTACGTACAT CCCGAAAGAC AGCAAAAAGA AGAAGCACGA GCTGAAGATT
    ACTCAGCAGG GCACGGACCC GCTTGTTCTC GCCGTCCAGA GCAAGGAACA GGCCGAGCAG
    TGGCTGAAGG TGATCAAAGA AGCCTACAGT GGTTGTAGTG GCCCCGTGGA TTCAGAGTGT
    CCTCCTCCAC CAAGCTCCCC GGTGCACAAG GCAGAACTGG AGAAGAAACT GTCTTCAGAG
    AGACCCAGCT CAGATGGGGA GGGTGTTGTG GAAAATGGAA TTACCACATG TAATGGAAAG
    GAGCAAGTGA AGAGGAAGAA AAGTTCCAAA TCAGAGGCCA AGGGCACTGT GTCGAAAGTC
    ACTGGGAAAA AAATCACCAA GATCATCAGT CTGGGAAAGA AAAAGCCGTC CACAGACGAG
    CAGACCTCCT CAGCTGAGGA AGATGTTCCC ACCTGCGGCT ATCTGAACGT GCTCTCCAAC
    AGCCGCTGGC GAGAGCGCTG GTGCCGAGTG AAAGATAACA AGCTCATTTT CCACAAGGAC
    AGGACCGACC TGAAGACCCA TATTGTGTCT ATTCCGCTCC GTGGCTGCGA GGTGATCCCG
    GGTTTGGATT CTAAACATCC TCTGACGTTC CGGCTGCTGC GCAACGGCCA GGAGGTTGCA
    GTATTGGAGG CATCTTCTTC TGAAGACATG GGCAGGTGGA TTGGGATTTT ACTCGCAGAG
    ACGGGATCGT CCACAGACCC GGAGGCTCTG CACTATGACT ACATTGATGT GGAGATGTCT
    GCAAGTGTCA TTCAGACAGC CAAACAGACC TTCTGTTTCA TGAACAGGCG TGTTATATCT
    GCTAACCCAT ATCTAGGGGG CACCTCCAAC GGCTATGCCC ACCCCAGCGG GACGGCACTT
    CATTATGACG ATGTCCCGTG CATCAACGGC TCGCTCAAGG GTAAAAAGCC CCCCGTGGCG
    TCTAATGGGG TCACAGGAAA AGGGAAGACT CTGAGCAGTC AGCCAAAGAA AGCGGATCCC
    GCGGCTGTTG TGAAAAGGAC GGGTTCGAAT GCTGCCCAGT ACAAGTATGG CAAGAACCGG
    GTAGAAGCAG ATGCCAAGCG GCTACAGACC AAAGAGGAGG AGCTGCTGAA GAGGAAAGAG
    GCCCTGCGGA ATAGGCTGGC CCAGCTCCGC AAGGAAAGAA AAGACCTTCG AGCGGCTATT
    GAAGTGAACG CCGGCAGGAA GCCGCAGGCG ATCCTGGAGG AGAAGCTGAA GCAGCTGGAG
    GAGGAGTGCC GGCAGAAGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACGGAGGTC
    AAGGAGAGCC TGAAGAAAGC GCTGGCGGGC GGAGTCACCC TGGGGCTGGC CATCGAGCCC
    AAGTCAGGGA CATCGAGTCC ACAGTCTCCA GTGTTCCGGC ACCGGACCCT GGAAAACTCG
    CCCATCTCCA GCTGTGACAC CAGTGACACC GAGGGCCCCG TGCCGGTGAA CAGCGCGGCC
    GTCTTGAAGA AGAGCCAGGC TGCCCCGGGC AGCTCCCCCT GCCGAGGGCA TGTGCTGCGG
    AAGGCCAAGG AATGGGAATT GAAGAACGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713972.1
    CDS94..2349
    Translation

    Target ORF information:

    RefSeq Version XM_006713909.3
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006713909.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCGTTTCA AGCAGGAGCA TCCACACCCA CGGAATGAGG CCAAGGCAGT TACAGTTAGA 
    GCAATGGAAG AGTTAATAGT TGAACTTCGT CTCTTTCTTG AACTCCTGGA CCATGAATAT
    CTAACCTCAA CTGTCAGGGA GAAAAAGGCA GTGATAACCA ACATTCTGCT AAGAATACAG
    TCATCCAAAG GTTTTGATGT GAAGGACCAT GCTCAGAAGC AGGAGACCGC TAACAGCCTG
    CCAGCCCCTC CTCAGATGCC CCTGCCGGAG ATCCCTCAGC CCTGGCTGCC TCCTGACAGT
    GGGCCTCCAC CATTGCCAAC ATCCTCCCTC CCAGAAGGTT ATTATGAGGA AGCTGTGCCG
    CTGAGCCCCG GAAAAGCTCC GGAATACATC ACATCAAATT ATGATTCCGA TGCGATGAGC
    AGCTCTTATG AGTCGTATGA TGAAGAGGAG GAGGATGGGA AGGGGAAGAA AACCCGGCAC
    CAGTGGCCCT CCGAGGAGGC CTCCATGGAC CTGGTCAAGG ACGCCAAAAT CTGCGCCTTC
    CTGCTGCGGA AGAAGCGGTT CGGCCAGTGG ACCAAGTTGC TCTGCGTCAT CAAAGACACC
    AAACTGCTGT GCTATAAAAG TTCCAAGGAC CAGCAGCCTC AGATGGAACT GCCACTCCAA
    GGCTGTAACA TTACGTACAT CCCGAAAGAC AGCAAAAAGA AGAAGCACGA GCTGAAGATT
    ACTCAGCAGG GCACGGACCC GCTTGTTCTC GCCGTCCAGA GCAAGGAACA GGCCGAGCAG
    TGGCTGAAGG TGATCAAAGA AGCCTACAGT GGTTGTAGTG GCCCCGTGGA TTCAGAGTGT
    CCTCCTCCAC CAAGCTCCCC GGTGCACAAG GCAGAACTGG AGAAGAAACT GTCTTCAGAG
    AGACCCAGCT CAGATGGGGA GGGTGTTGTG GAAAATGGAA TTACCACATG TAATGGAAAG
    GAGCAAGTGA AGAGGAAGAA AAGTTCCAAA TCAGAGGCCA AGGGCACTGT GTCGAAAGTC
    ACTGGGAAAA AAATCACCAA GATCATCAGT CTGGGAAAGA AAAAGCCGTC CACAGACGAG
    CAGACCTCCT CAGCTGAGGA AGATGTTCCC ACCTGCGGCT ATCTGAACGT GCTCTCCAAC
    AGCCGCTGGC GAGAGCGCTG GTGCCGAGTG AAAGATAACA AGCTCATTTT CCACAAGGAC
    AGGACCGACC TGAAGACCCA TATTGTGTCT ATTCCGCTCC GTGGCTGCGA GGTGATCCCG
    GGTTTGGATT CTAAACATCC TCTGACGTTC CGGCTGCTGC GCAACGGCCA GGAGGTTGCA
    GTATTGGAGG CATCTTCTTC TGAAGACATG GGCAGGTGGA TTGGGATTTT ACTCGCAGAG
    ACGGGATCGT CCACAGACCC GGAGGCTCTG CACTATGACT ACATTGATGT GGAGATGTCT
    GCAAGTGTCA TTCAGACAGC CAAACAGACC TTCTGTTTCA TGAACAGGCG TGTTATATCT
    GCTAACCCAT ATCTAGGGGG CACCTCCAAC GGCTATGCCC ACCCCAGCGG GACGGCACTT
    CATTATGACG ATGTCCCGTG CATCAACGGC TCGCTCAAGG GTAAAAAGCC CCCCGTGGCG
    TCTAATGGGG TCACAGGAAA AGGGAAGACT CTGAGCAGTC AGCCAAAGAA AGCGGATCCC
    GCGGCTGTTG TGAAAAGGAC GGGTTCGAAT GCTGCCCAGT ACAAGTATGG CAAGAACCGG
    GTAGAAGCAG ATGCCAAGCG GCTACAGACC AAAGAGGAGG AGCTGCTGAA GAGGAAAGAG
    GCCCTGCGGA ATAGGCTGGC CCAGCTCCGC AAGGAAAGAA AAGACCTTCG AGCGGCTATT
    GAAGTGAACG CCGGCAGGAA GCCGCAGGCG ATCCTGGAGG AGAAGCTGAA GCAGCTGGAG
    GAGGAGTGCC GGCAGAAGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACGGAGGTC
    AAGGAGAGCC TGAAGAAAGC GCTGGCGGGC GGAGTCACCC TGGGGCTGGC CATCGAGCCC
    AAGTCAGGGA CATCGAGTCC ACAGTCTCCA GTGTTCCGGC ACCGGACCCT GGAAAACTCG
    CCCATCTCCA GCTGTGACAC CAGTGACACC GAGGGCCCCG TGCCGGTGAA CAGCGCGGCC
    GTCTTGAAGA AGAGCCAGGC TGCCCCGGGC AGCTCCCCCT GCCGAGGGCA TGTGCTGCGG
    AAGGCCAAGG AATGGGAATT GAAGAACGGG ACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95369
    Clone ID Related Accession (Same CDS sequence) XM_017008535.1
    Accession Version XM_017008535.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGCTCAAG GGTAAAAAGC CCCCCGTGGC GTCTAATGGG
    GTCACAGGAA AAGGGAAGAC TCTGAGCAGT CAGCCAAAGA AAGCGGATCC CGCGGCTGTT
    GTGAAAAGGA CGGGTTCGAA TGCTGCCCAG TACAAGTATG GCAAGAACCG GGTAGAAGCA
    GATGCCAAGC GGCTACAGAC CAAAGAGGAG GAGCTGCTGA AGAGGAAAGA GGCCCTGCGG
    AATAGGCTGG CCCAGCTCCG CAAGGAAAGA AAAGACCTTC GAGCGGCTAT TGAAGTGAAC
    GCCGGCAGGA AGCCGCAGGC GATCCTGGAG GAGAAGCTGA AGCAGCTGGA GGAGGAGTGC
    CGGCAGAAGG AGGCGGAGCG TGTCAGCCTG GAGCTGGAGC TGACGGAGGT CAAGGAGAGC
    CTGAAGAAAG CGCTGGCGGG CGGAGTCACC CTGGGGCTGG CCATCGAGCC CAAGTCAGGG
    ACATCGAGTC CACAGTCTCC AGTGTTCCGG CACCGGACCC TGGAAAACTC GCCCATCTCC
    AGCTGTGACA CCAGTGACAC CGAGGGCCCC GTGCCGGTGA ACAGCGCGGC CGTCTTGAAG
    AAGAGCCAGG CTGCCCCGGG CAGCTCCCCC TGCCGAGGGC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAAT TGAAGAACGG GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864024.1
    CDS3..2249
    Translation

    Target ORF information:

    RefSeq Version XM_017008535.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008535.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGCTCAAG GGTAAAAAGC CCCCCGTGGC GTCTAATGGG
    GTCACAGGAA AAGGGAAGAC TCTGAGCAGT CAGCCAAAGA AAGCGGATCC CGCGGCTGTT
    GTGAAAAGGA CGGGTTCGAA TGCTGCCCAG TACAAGTATG GCAAGAACCG GGTAGAAGCA
    GATGCCAAGC GGCTACAGAC CAAAGAGGAG GAGCTGCTGA AGAGGAAAGA GGCCCTGCGG
    AATAGGCTGG CCCAGCTCCG CAAGGAAAGA AAAGACCTTC GAGCGGCTAT TGAAGTGAAC
    GCCGGCAGGA AGCCGCAGGC GATCCTGGAG GAGAAGCTGA AGCAGCTGGA GGAGGAGTGC
    CGGCAGAAGG AGGCGGAGCG TGTCAGCCTG GAGCTGGAGC TGACGGAGGT CAAGGAGAGC
    CTGAAGAAAG CGCTGGCGGG CGGAGTCACC CTGGGGCTGG CCATCGAGCC CAAGTCAGGG
    ACATCGAGTC CACAGTCTCC AGTGTTCCGG CACCGGACCC TGGAAAACTC GCCCATCTCC
    AGCTGTGACA CCAGTGACAC CGAGGGCCCC GTGCCGGTGA ACAGCGCGGC CGTCTTGAAG
    AAGAGCCAGG CTGCCCCGGG CAGCTCCCCC TGCCGAGGGC ATGTGCTGCG GAAGGCCAAG
    GAATGGGAAT TGAAGAACGG GACCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95370
    Clone ID Related Accession (Same CDS sequence) XM_017008536.1
    Accession Version XM_017008536.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product actin filament-associated protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTCTC AAGGGTAAAA
    AGCCCCCCGT GGCGTCTAAT GGGGTCACAG GAAAAGGGAA GACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864025.1
    CDS3..1670
    Translation

    Target ORF information:

    RefSeq Version XM_017008536.1
    Organism Homo sapiens(human)
    Definition Homo sapiens actin filament associated protein 1 (AFAP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008536.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAGAGT TAATAGTTGA ACTTCGTCTC TTTCTTGAAC TCCTGGACCA TGAATATCTA 
    ACCTCAACTG TCAGGGAGAA AAAGGCAGTG ATAACCAACA TTCTGCTAAG AATACAGTCA
    TCCAAAGGTT TTGATGTGAA GGACCATGCT CAGAAGCAGG AGACCGCTAA CAGCCTGCCA
    GCCCCTCCTC AGATGCCCCT GCCGGAGATC CCTCAGCCCT GGCTGCCTCC TGACAGTGGG
    CCTCCACCAT TGCCAACATC CTCCCTCCCA GAAGGTTATT ATGAGGAAGC TGTGCCGCTG
    AGCCCCGGAA AAGCTCCGGA ATACATCACA TCAAATTATG ATTCCGATGC GATGAGCAGC
    TCTTATGAGT CGTATGATGA AGAGGAGGAG GATGGGAAGG GGAAGAAAAC CCGGCACCAG
    TGGCCCTCCG AGGAGGCCTC CATGGACCTG GTCAAGGACG CCAAAATCTG CGCCTTCCTG
    CTGCGGAAGA AGCGGTTCGG CCAGTGGACC AAGTTGCTCT GCGTCATCAA AGACACCAAA
    CTGCTGTGCT ATAAAAGTTC CAAGGACCAG CAGCCTCAGA TGGAACTGCC ACTCCAAGGC
    TGTAACATTA CGTACATCCC GAAAGACAGC AAAAAGAAGA AGCACGAGCT GAAGATTACT
    CAGCAGGGCA CGGACCCGCT TGTTCTCGCC GTCCAGAGCA AGGAACAGGC CGAGCAGTGG
    CTGAAGGTGA TCAAAGAAGC CTACAGTGGT TGTAGTGGCC CCGTGGATTC AGAGTGTCCT
    CCTCCACCAA GCTCCCCGGT GCACAAGGCA GAACTGGAGA AGAAACTGTC TTCAGAGAGA
    CCCAGCTCAG ATGGGGAGGG TGTTGTGGAA AATGGAATTA CCACATGTAA TGGAAAGGAG
    CAAGTGAAGA GGAAGAAAAG TTCCAAATCA GAGGCCAAGG GCACTGTGTC GAAAGTCACT
    GGGAAAAAAA TCACCAAGAT CATCAGTCTG GGAAAGAAAA AGCCGTCCAC AGACGAGCAG
    ACCTCCTCAG CTGAGGAAGA TGTTCCCACC TGCGGCTATC TGAACGTGCT CTCCAACAGC
    CGCTGGCGAG AGCGCTGGTG CCGAGTGAAA GATAACAAGC TCATTTTCCA CAAGGACAGG
    ACCGACCTGA AGACCCATAT TGTGTCTATT CCGCTCCGTG GCTGCGAGGT GATCCCGGGT
    TTGGATTCTA AACATCCTCT GACGTTCCGG CTGCTGCGCA ACGGCCAGGA GGTTGCAGTA
    TTGGAGGCAT CTTCTTCTGA AGACATGGGC AGGTGGATTG GGATTTTACT CGCAGAGACG
    GGATCGTCCA CAGACCCGGA GGCTCTGCAC TATGACTACA TTGATGTGGA GATGTCTGCA
    AGTGTCATTC AGACAGCCAA ACAGACCTTC TGTTTCATGA ACAGGCGTGT TATATCTGCT
    AACCCATATC TAGGGGGCAC CTCCAACGGC TATGCCCACC CCAGCGGGAC GGCACTTCAT
    TATGACGATG TCCCGTGCAT CAACGGCTCG TGGGAACCGG AAGACGGCTT TCCTGCTTCC
    TGCAGCAGAG GCTTGGGAGA AGAGGTGCTT TATGATAACG CAGGCCTCTC AAGGGTAAAA
    AGCCCCCCGT GGCGTCTAAT GGGGTCACAG GAAAAGGGAA GACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.