Afap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afap1
Entrez Gene ID	140935
Full Name	actin filament associated protein 1
Synonyms	Afap
General protein information	Preferred Names actin filament associated protein 1 Names actin filament-associated protein 1 110 kDa actin filament-associated protein AFAP-110
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599057.1	XP_017454546.1	actin filament-associated protein 1 isoform X1
NM_080900.1	NP_543176.1	actin filament-associated protein 1
XM_008770245.2	XP_008768467.1	actin filament-associated protein 1 isoform X3
XM_008770246.2	XP_008768468.1	actin filament-associated protein 1 isoform X4
XM_008770247.2	XP_008768469.1	actin filament-associated protein 1 isoform X2

Pathways from BioSystems

Homologies

	LOC709419	XP_002804046.1
Xenopus tropicalis (tropical clawed frog)	afap1	XP_004911301.1
Mus musculus (house mouse)	Afap1	NP_081649.1
Gallus gallus (chicken)	AFAP1	NP_989536.1
Bos taurus (cattle)	AFAP1	NP_001192973.1
Rattus norvegicus (Norway rat)	Afap1	NP_543176.1
Danio rerio (zebrafish)	afap1	XP_005156284.1
Homo sapiens (human)	AFAP1	NP_001128119.1
Pan troglodytes (chimpanzee)	AFAP1	XP_003310294.1
Canis lupus familiaris (dog)	AFAP1	XP_005618516.1

Gene Ontology
Bibliography

Related articles in PubMed

AFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in Osteoblasts.
Cho Y, Silverstein R, Geisinger MT, Martinkovich S, Corkill H, Cunnick JM, Planey SL, Arnott JA
PloS one10(9)e0136712(2015)

Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma.
Gharahkhani P, Burdon KP, Fogarty R, Sharma S, Hewitt AW, Martin S, Law MH, Cremin K, Bailey JNC, Loomis SJ, Pasquale LR, Haines JL, Hauser MA, Viswanathan AC, McGuffin P, Topouzis F, Foster PJ, Graham SL, Casson RJ, Chehade M, White AJ, Zhou T, Souzeau E, Landers J, Fitzgerald JT, Klebe S, Ruddle JB, Goldberg I, Healey PR, , Mills RA, Wang JJ, Montgomery GW, Martin NG, RadfordSmith G, Whiteman DC, Brown MA, Wiggs JL, Mackey DA, Mitchell P, MacGregor S, Craig JE
Nature genetics46(10)1120-1125(2014 Oct)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.
Crimaldi L, Courtneidge SA, Gimona M
Experimental cell research315(15)2581-92(2009 Sep)

Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells.
Dorfleutner A, Cho Y, Vincent D, Cunnick J, Lin H, Weed SA, Stehlik C, Flynn DC
Journal of cell science121(Pt 14)2394-405(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AFAP1 is an essential downstream signaling component of TGF-beta1 for Src activation, CCN2 induction and collagen XIIa in osteoblasts.
Title: AFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in Osteoblasts.

Results demonstrate that Tks5 plays a central role in the recruitment of AFAP-110, p190RhoGAP, and cortactin to drive podosome formation.
Title: Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.

AFAP-110 phosphorylation and/or dephosphorylation is involved in the regulation of podosome stability and lifespan.
Title: Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells.

The following Afap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42487	NM_080900.1 Latest version!	Rattus norvegicus actin filament associated protein 1 (Afap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44193	XM_008770245.2 Latest version!	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44194	XM_008770246.2 Latest version!	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa44195	XM_008770247.2 Latest version!	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa58210	XM_017599057.1 Latest version!	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afap1 ( NM_080900.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_543176.1 Rattus norvegicus actin filament associated protein 1 (Afap1), mRNA.

Price & Availability↑

CloneID	ORa42487
Clone ID Related Accession (Same CDS sequence)	NM_080900.1
Accession Version	NM_080900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	actin filament-associated protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY063759.1. On Jan 12, 2005 this sequence version replaced XM_346465.1. ##Evidence-Data-START## Transcript exon combination :: AY063759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAAGAGT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCTCCTCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTTCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GGGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCA GCATCAGTGC 
CATCATGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAGGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATCCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCT 
ACTAGCCCGT ACCTAGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGCGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAGGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GCTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAGGG AGTGGGAGCT GAAGAATGGA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543176.1
CDS	1..2196
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGFEVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSEAMGSSYESYDEEEEDGKGKKTQHQCHHEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKKLSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGLPVKGRAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAREWELKNGT

Target ORF information:

RefSeq Version	NM_080900.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin filament associated protein 1 (Afap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080900.1

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCTCCTCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTTCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GGGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCA GCATCAGTGC 
CATCATGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAGGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATCCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCT 
ACTAGCCCGT ACCTAGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGCGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAGGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GCTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAGGG AGTGGGAGCT GAAGAATGGA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_008770245.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768467.1 Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44193
Clone ID Related Accession (Same CDS sequence)	XM_008770245.2
Accession Version	XM_008770245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin filament-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAAGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAAGG TGAGCCCCCT GGCAGGGCCA GGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768467.1
CDS	159..2357
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGFEVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSEAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKKLSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGLPVKGKAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAKVSPLAGPGT

Target ORF information:

RefSeq Version	XM_008770245.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770245.2

ORF Insert Sequence:

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAAGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAAGG TGAGCCCCCT GGCAGGGCCA GGTACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_008770246.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768468.1 Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa44194
Clone ID Related Accession (Same CDS sequence)	XM_008770246.2
Accession Version	XM_008770246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin filament-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAAGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAAGG AGTGGGAGCT GAAGAATGGA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768468.1
CDS	158..2353
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGFEVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSEAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKKLSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGLPVKGKAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_008770246.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770246.2

ORF Insert Sequence:

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC TCCAGCGTCA 
TCTAATGGGC TTCCTGTGAA GGGAAAGGCT CCCAGCAGCC AGCAAAAAAA GGTGGAATCT 
GCGGGGGGCG TGAAGCGCAC AGCATCAAAT GCCGAGCAGT ACAAATATGG TAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTACTGAA GAGAAAAGAG 
GCCCTGCGGA ACAGGCTGGC ACAGCTCCGG AAAGAGAGGA AGGACCTGAG GGCTGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGTG GCCCTGGAGG ACAAGTTGAA GAGGTTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCAGAGCGG GTCAGCCTGG AGCTGGAGCT GACGGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCAGGT GGCGTCACTC TAGGACTGGC TATCGAACCC 
AAGTCAGGGA CATCAAGCCC ACAGTCTCCA GTGTTTCGGC ATCGGACTCT GGAAAACTCC 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCCAGC AGCTCCCCTT GCCGTGGGCA CGTGCTGCAG 
AAGGCCAAGG AGTGGGAGCT GAAGAATGGA ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_008770247.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768469.1 Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44195
Clone ID Related Accession (Same CDS sequence)	XM_008770247.2
Accession Version	XM_008770247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGGGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCAGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTATCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCGT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCTCC AGCGTCATCT AATGGGCTTC CTGTGAAGGG AAAGGCTCCC 
AGCAGCCAGC AAAAAAAGGT GGAATCTGCG GGGGGCGTGA AGCGCACAGC ATCAAATGCC 
GAGCAGTACA AATATGGTAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TACTGAAGAG AAAAGAGGCC CTGCGGAACA GGCTGGCACA GCTCCGGAAA 
GAGAGGAAGG ACCTGAGGGC TGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGTGGCC 
CTGGAGGACA AGTTGAAGAG GTTGGAGGAA GAGTGCAAGC AGAGGGAGGC AGAGCGGGTC 
AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCAGGTGGC 
GTCACTCTAG GACTGGCTAT CGAACCCAAG TCAGGGACAT CAAGCCCACA GTCTCCAGTG 
TTTCGGCATC GGACTCTGGA AAACTCCCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCCAGCAGC 
TCCCCTTGCC GTGGGCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768469.1
CDS	161..2623
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGFEVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSEAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKKLSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCGSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGLPVKGKAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_008770247.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770247.2

ORF Insert Sequence:

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGGGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCAGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTATCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCGT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCTCC AGCGTCATCT AATGGGCTTC CTGTGAAGGG AAAGGCTCCC 
AGCAGCCAGC AAAAAAAGGT GGAATCTGCG GGGGGCGTGA AGCGCACAGC ATCAAATGCC 
GAGCAGTACA AATATGGTAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TACTGAAGAG AAAAGAGGCC CTGCGGAACA GGCTGGCACA GCTCCGGAAA 
GAGAGGAAGG ACCTGAGGGC TGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGTGGCC 
CTGGAGGACA AGTTGAAGAG GTTGGAGGAA GAGTGCAAGC AGAGGGAGGC AGAGCGGGTC 
AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCAGGTGGC 
GTCACTCTAG GACTGGCTAT CGAACCCAAG TCAGGGACAT CAAGCCCACA GTCTCCAGTG 
TTTCGGCATC GGACTCTGGA AAACTCCCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCCAGCAGC 
TCCCCTTGCC GTGGGCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGAACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_017599057.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454546.1 Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58210
Clone ID Related Accession (Same CDS sequence)	XM_017599057.1
Accession Version	XM_017599057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGGGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCAGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTATCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCGT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCTCC AGCGTCATCT AATGGGCTTC CTGTGAAGGG AAAGGCTCCC 
AGCAGCCAGC AAAAAAAGGT GGAATCTGCG GGGGGCGTGA AGCGCACAGC ATCAAATGCC 
GAGCAGTACA AATATGGTAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TACTGAAGAG AAAAGAGGCC CTGCGGAACA GGCTGGCACA GCTCCGGAAA 
GAGAGGAAGG ACCTGAGGGC TGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGTGGCC 
CTGGAGGACA AGTTGAAGAG GTTGGAGGAA GAGTGCAAGC AGAGGGAGGC AGAGCGGGTC 
AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCAGGTGGC 
GTCACTCTAG GACTGGCTAT CGAACCCAAG TCAGGGACAT CAAGCCCACA GTCTCCAGTG 
TTTCGGCATC GGACTCTGGA AAACTCCCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCCAGCAGC 
TCCCCTTGCC GTGGGCACGT GCTGCAGAAG GCCAAGGTGA GCCCCCTGGC AGGGCCAGGT 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454546.1
CDS	161..2626
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRMQSSKGFEVKDHAQKAETNNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSEAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSASVPVNKAELEKKLSSERPSSDGEGVVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHIVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCGSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGLPVKGKAPSSQQKKVESAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQVALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPKSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSSSSPCRGHVLQKAKVSPLAGPGT

Target ORF information:

RefSeq Version	XM_017599057.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599057.1

ORF Insert Sequence:

ATGGAAGAAT TAATAGTTGA ACTCCGCCTG TTCCTGGAGC TCCTGGACCA TGAGTACCTC 
ACGTCAACTG TCAGAGAGAA AAAGGCAGTG CTTACCAACA TCCTGCTTCG AATGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCA CAGAAGGCAG AGACCAACAA CCTCCCGGCA 
CCACCCCAGA TGCCTCTGCC AGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGTGGGCCT 
CCGCCGTTAC CGACATCCTC TCTCCCAGAG GGTTACTATG AGGAAGCTGT GCCCCTGAGT 
CCTGGAAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGAGGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAT GGGAAGGGGA AGAAAACGCG GCATCAGTGG 
CCATCAGAGG AGGCCTCTAT GGACCTGGTG AAAGATGCCA AGATCTGCGC CTTCCTGTTG 
CGTAAGAAAC GCTTCGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTACA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCCCT GCAGGGCTGC 
AGCATCACGT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT CCTCGCCGTC CAGAGCAAGG AGCAGGCCGA GCAGTGGCTG 
AAGGTAATCA AAGAGGCCTA CAGTGGCTGC AGTGGGCCCG TGGATCCAGA GTGTTCTCCA 
CCACCCAGCG CCAGTGTCCC TGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGTTGTA GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCCTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACGTCAT CAGCAGAGGA AGATGTGCCC ACCTGTGGCT ACCTAAATGT GCTCTCTAAC 
AGTCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AGCTCATTCT CCACAAGGAC 
CGGGCTGACC TGAAGACCCA CATCGTCTCC ATCCCTCTCC GCGGCTGCGA GGTGATTCCG 
GGCTTGGATT CTAAGCACCC ATTGACCTTC CGGCTGCTTC GAAATGGACA GGAGGTGGCT 
GTCCTAGAGG CATCATCTTC TGAAGACATG GGCAGATGGA TTGGCATCTT ACTGGCTGAG 
ACAGGGTCCT CAACGGACCC AGGAGCCCTA CACTATGACT ACATAGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC TAAACAGACC TTCTGCTTTA TGAACAGGAG GGCAGTGTCC 
ACTAGCCCGT ACCTGGGGAG CCTCTCCAAT GGCTATGCCC ATCCCAGTGG GACAGCACTA 
CACTATGACG ACGTCCCTTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGGGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCAGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTATCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCGT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCTCC AGCGTCATCT AATGGGCTTC CTGTGAAGGG AAAGGCTCCC 
AGCAGCCAGC AAAAAAAGGT GGAATCTGCG GGGGGCGTGA AGCGCACAGC ATCAAATGCC 
GAGCAGTACA AATATGGTAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TACTGAAGAG AAAAGAGGCC CTGCGGAACA GGCTGGCACA GCTCCGGAAA 
GAGAGGAAGG ACCTGAGGGC TGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGTGGCC 
CTGGAGGACA AGTTGAAGAG GTTGGAGGAA GAGTGCAAGC AGAGGGAGGC AGAGCGGGTC 
AGCCTGGAGC TGGAGCTGAC GGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCAGGTGGC 
GTCACTCTAG GACTGGCTAT CGAACCCAAG TCAGGGACAT CAAGCCCACA GTCTCCAGTG 
TTTCGGCATC GGACTCTGGA AAACTCCCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCCAGCAGC 
TCCCCTTGCC GTGGGCACGT GCTGCAGAAG GCCAAGGTGA GCCCCCTGGC AGGGCCAGGT 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AFAP1 Is a Novel Downstream Mediator of TGF-?1 for CCN2 Induction in Osteoblasts.
PloS one10(9)e0136712(2015)
Cho Y,Silverstein R,Geisinger MT,Martinkovich S,Corkill H,Cunnick JM,Planey SL,Arnott JA

Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma.
Nature genetics46(10)1120-1125(2014 Oct)
Gharahkhani P,Burdon KP,Fogarty R,Sharma S,Hewitt AW,Martin S,Law MH,Cremin K,Bailey JNC,Loomis SJ,Pasquale LR,Haines JL,Hauser MA,Viswanathan AC,McGuffin P,Topouzis F,Foster PJ,Graham SL,Casson RJ,Chehade M,White AJ,Zhou T,Souzeau E,Landers J,Fitzgerald JT,Klebe S,Ruddle JB,Goldberg I,Healey PR,,Mills RA,Wang JJ,Montgomery GW,Martin NG,RadfordSmith G,Whiteman DC,Brown MA,Wiggs JL,Mackey DA,Mitchell P,MacGregor S,Craig JE

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.
Experimental cell research315(15)2581-92(2009 Sep)
Crimaldi L,Courtneidge SA,Gimona M

Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells.
Journal of cell science121(Pt 14)2394-405(2008 Jul)
Dorfleutner A,Cho Y,Vincent D,Cunnick J,Lin H,Weed SA,Stehlik C,Flynn DC

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Afap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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