MAN2A2 cDNA ORF clone, Canis lupus familiaris(dog)

The following MAN2A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287230 XM_022416947.1
Latest version!
Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe287229 XM_022416946.1
Latest version!
Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe04298 XM_022416944.1
Latest version!
Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe04298 XM_005618371.2
Latest version!
Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe04299 XM_022416945.1
Latest version!
Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04299 XM_545863.4
Latest version!
Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe04298 XM_005618371.1 Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287230
    Clone ID Related Accession (Same CDS sequence) XM_022416947.1
    Accession Version XM_022416947.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-mannosidase 2x isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GACAGCGCTC AGTGATGCCT
    AATTTGAGCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272655.1
    CDS346..3240
    Translation

    Target ORF information:

    RefSeq Version XM_022416947.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416947.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GACAGCGCTC AGTGATGCCT
    AATTTGAGCT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287229
    Clone ID Related Accession (Same CDS sequence) XM_022416946.1
    Accession Version XM_022416946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAAGT AATCATCAAT
    GCTGCTCACT ATCTGGTGTT GGGGGACAAG GAGACCTACC ACTTCGACCC TGAGGCACCC
    TTCCTCCAAA TGGATGAAAC CCGCTTAAAT CACGACACAC TGCCAGAGCG CACAGTGATC
    CAGCTAGATT CCTCACCCAG GTATGTGGTG CTCTTCAACC CGCTGGAACA GGAGCGGCTG
    AGTGTGGTGT CCCTGTTGGT CAGCTCGCCC CGGGTGCGGG TCCTTTCAGA GGAGGGCCAG
    CCCCTGGCTG TGCAGATCAG TGCACACTGG AGCTCTGCCA CCGACATGGT CTCTGATGTC
    TGCCAGGTAT CTATGCCCAT CCGCCTGCCG GCCCTGGGCC TCAGCGTGCT ACAGCTGCAG
    CTGGGCCTCG ATGGGCACCG CACCCTGCCC TCCTCTGTGC GTGTCTACCT GCATGGCCGT
    CAGCTGGCCG TCAGCAGGCA GGACGCTTTC CCACTGCGCA TCATTGACTC TGGCACCAGT
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC CGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA CGAGGAGCAG GAGCGGCGGG TGGACATGGA GTTCCTCATC
    TATGGCACCC GCGCGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC TGACAGGGAG
    GCCCAGCCCT ATATCCCCAA GGACCCCCCC GTGCTGCGTG TCACCGAAGG CCCTTTCTTC
    TCGGAGGTGG TGGCATACTA CGAGCATATT CGCCAGGTGG TCCGGCTATA TAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATGTCG TCCTTGGTGG ACATCCGGGA TTACATCAAC
    AAGGAGCTGG CCCTGCGCAT CCACACAGAC ATTGACAGCC AGGGTGCCTT CTTCACGGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGGCGC TATCTGAAGA AGCTGCCTCT GCAGGCCAAT
    TTCTACCCCA TGCCAGTCAT GGCCTACATC CAGGATGCGC AGAACCGCCT CACACTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCCAGTCTG CACGATGGCC AGCTGGAGGT GATCCTGGAC
    CGACGGCTCA TGCAAGATGA CAACCGGGGT CTGGGCCAAG GGCTCAAGGA CAATAAAAGA
    ACCTGCAACC ATTTCCGCCT CCTGTTGGAA CGGCGAACCC TGGGCAGTGA GCCTGGCTTT
    TTCTCCAAAC TGGCAGCCAT GTTTAGGGAC TTGATCTTTC ACAGCAGCAG GACCAATAAC
    CTTGAGGTCC AGGATGGCCA CACCAGCAGC TACCCGTCCC TCCTCAGCCA CCTGACCTCC
    ATGTACCTGA ATATCCCAGT GTTTGCCCTG CCTGTGGCCA AGAGGCAGCT CCCCGGCCCT
    GGTCTACGCT CCTTTCATCC CCTGGCCTCC TCCCTGCCCT GTGACTTCCA CCTGCTCAAC
    CTGCGCACCC TGCAGGCCGA GGATGATGCC TTGCCATCAG CAGAGACCGC ACTCATCTTA
    CACCGCAAGG GTTTTGACTG TGGCCTTGAG GCCAAGAACT TGGGCTTCAA CTGCAGCACA
    AGCCAAGGCA AGGCAGCCCT GGGGAGCCTC TTCCATGGCC TGGATGTAGG TTTCCTGCAG
    CCGACCTCAC TGACATTACT GTACCCACTG GCCGCACCCT CCAACAGCAC TGATGTCTAC
    CTGGAGCCCA TGGAGATTGC CACCTTTCGC CTCCGCTTGG GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272654.1
    CDS346..3690
    Translation

    Target ORF information:

    RefSeq Version XM_022416946.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGCT TCTGCGCTCC CTTGTCAACC TGAAGCAAGT AATCATCAAT
    GCTGCTCACT ATCTGGTGTT GGGGGACAAG GAGACCTACC ACTTCGACCC TGAGGCACCC
    TTCCTCCAAA TGGATGAAAC CCGCTTAAAT CACGACACAC TGCCAGAGCG CACAGTGATC
    CAGCTAGATT CCTCACCCAG GTATGTGGTG CTCTTCAACC CGCTGGAACA GGAGCGGCTG
    AGTGTGGTGT CCCTGTTGGT CAGCTCGCCC CGGGTGCGGG TCCTTTCAGA GGAGGGCCAG
    CCCCTGGCTG TGCAGATCAG TGCACACTGG AGCTCTGCCA CCGACATGGT CTCTGATGTC
    TGCCAGGTAT CTATGCCCAT CCGCCTGCCG GCCCTGGGCC TCAGCGTGCT ACAGCTGCAG
    CTGGGCCTCG ATGGGCACCG CACCCTGCCC TCCTCTGTGC GTGTCTACCT GCATGGCCGT
    CAGCTGGCCG TCAGCAGGCA GGACGCTTTC CCACTGCGCA TCATTGACTC TGGCACCAGT
    GACTTCGCCC TCAGCAACCG CTACATGCAG GTCTGGTTCT CAGGCCTTAC CGGGCTCCTC
    AAGAGCATCC GAAGGGTGGA CGAGGAGCAG GAGCGGCGGG TGGACATGGA GTTCCTCATC
    TATGGCACCC GCGCGTCCAA AGACAAGAGT GGAGCCTACC TCTTCCTGCC TGACAGGGAG
    GCCCAGCCCT ATATCCCCAA GGACCCCCCC GTGCTGCGTG TCACCGAAGG CCCTTTCTTC
    TCGGAGGTGG TGGCATACTA CGAGCATATT CGCCAGGTGG TCCGGCTATA TAATCTGCCA
    GGGGTGGAGG GGCTGTCTCT GGACATGTCG TCCTTGGTGG ACATCCGGGA TTACATCAAC
    AAGGAGCTGG CCCTGCGCAT CCACACAGAC ATTGACAGCC AGGGTGCCTT CTTCACGGAC
    CTCAATGGCT TTCAGGTGCA GCCCCGGCGC TATCTGAAGA AGCTGCCTCT GCAGGCCAAT
    TTCTACCCCA TGCCAGTCAT GGCCTACATC CAGGATGCGC AGAACCGCCT CACACTGCAC
    ACTGCCCAGG CCCTGGGTGT CTCCAGTCTG CACGATGGCC AGCTGGAGGT GATCCTGGAC
    CGACGGCTCA TGCAAGATGA CAACCGGGGT CTGGGCCAAG GGCTCAAGGA CAATAAAAGA
    ACCTGCAACC ATTTCCGCCT CCTGTTGGAA CGGCGAACCC TGGGCAGTGA GCCTGGCTTT
    TTCTCCAAAC TGGCAGCCAT GTTTAGGGAC TTGATCTTTC ACAGCAGCAG GACCAATAAC
    CTTGAGGTCC AGGATGGCCA CACCAGCAGC TACCCGTCCC TCCTCAGCCA CCTGACCTCC
    ATGTACCTGA ATATCCCAGT GTTTGCCCTG CCTGTGGCCA AGAGGCAGCT CCCCGGCCCT
    GGTCTACGCT CCTTTCATCC CCTGGCCTCC TCCCTGCCCT GTGACTTCCA CCTGCTCAAC
    CTGCGCACCC TGCAGGCCGA GGATGATGCC TTGCCATCAG CAGAGACCGC ACTCATCTTA
    CACCGCAAGG GTTTTGACTG TGGCCTTGAG GCCAAGAACT TGGGCTTCAA CTGCAGCACA
    AGCCAAGGCA AGGCAGCCCT GGGGAGCCTC TTCCATGGCC TGGATGTAGG TTTCCTGCAG
    CCGACCTCAC TGACATTACT GTACCCACTG GCCGCACCCT CCAACAGCAC TGATGTCTAC
    CTGGAGCCCA TGGAGATTGC CACCTTTCGC CTCCGCTTGG GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04298
    Clone ID Related Accession (Same CDS sequence) XM_022416944.1 , XM_005618371.1 , XM_005618371.2
    Accession Version XM_022416944.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272652.1
    CDS1..3528
    Translation

    Target ORF information:

    RefSeq Version XM_022416944.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416944.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04298
    Clone ID Related Accession (Same CDS sequence) XM_022416944.1 , XM_005618371.1 , XM_005618371.2
    Accession Version XM_005618371.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-mannosidase 2x isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618428.1
    CDS346..3873
    Translation

    Target ORF information:

    RefSeq Version XM_005618371.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04298
    Clone ID Related Accession (Same CDS sequence) XM_022416944.1 , XM_005618371.1 , XM_005618371.2
    Accession Version XM_005618371.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618428.1
    CDS346..3873
    Translation

    Target ORF information:

    RefSeq Version XM_005618371.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618371.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGCCTGGC
    TTTTTCTCCA AACTGGCAGC CATGTTTAGG GACTTGATCT TTCACAGCAG CAGGACCAAT
    AACCTTGAGG TCCAGGATGG CCACACCAGC AGCTACCCGT CCCTCCTCAG CCACCTGACC
    TCCATGTACC TGAATATCCC AGTGTTTGCC CTGCCTGTGG CCAAGAGGCA GCTCCCCGGC
    CCTGGTCTAC GCTCCTTTCA TCCCCTGGCC TCCTCCCTGC CCTGTGACTT CCACCTGCTC
    AACCTGCGCA CCCTGCAGGC CGAGGATGAT GCCTTGCCAT CAGCAGAGAC CGCACTCATC
    TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCAGC
    ACAAGCCAAG GCAAGGCAGC CCTGGGGAGC CTCTTCCATG GCCTGGATGT AGGTTTCCTG
    CAGCCGACCT CACTGACATT ACTGTACCCA CTGGCCGCAC CCTCCAACAG CACTGATGTC
    TACCTGGAGC CCATGGAGAT TGCCACCTTT CGCCTCCGCT TGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04299
    Clone ID Related Accession (Same CDS sequence) XM_545863.4 , XM_022416945.1
    Accession Version XM_545863.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product alpha-mannosidase 2x isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_545863.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG
    GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT
    ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC
    TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG
    CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG
    GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG
    ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG
    GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_545863.3
    CDS346..3798
    Translation

    Target ORF information:

    RefSeq Version XM_545863.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_545863.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG
    GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT
    ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC
    TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG
    CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG
    GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG
    ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG
    GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04299
    Clone ID Related Accession (Same CDS sequence) XM_545863.4 , XM_022416945.1
    Accession Version XM_022416945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG
    GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT
    ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC
    TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG
    CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG
    GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG
    ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG
    GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272653.1
    CDS1..3453
    Translation

    Target ORF information:

    RefSeq Version XM_022416945.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGTGT GGCCGTCTTC 
    TCACTCTACC TCATGCTGGA CCGAGTGCAG CATGATCCCA CCCGTCACCA GAATGGTGGG
    AACTTCCCCC GGAGCCAAAT TTCTGTGCTG CAGAACCGCA TTGAGCAGCT GGAGCAGCTG
    TTGGAGGAGA ACCATGAAAT CATTAGCCAC ATCAAGGACT CTGTGCTGGA GCTGACAGCC
    AACGCAGAGG GCCCGCCTGC CCTGCTGCCC TACTACATGG CCAATGGCTC CTGGGTGGTG
    CCACCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGATTGCCA GTTTGCTTTG
    GGGGGCCGAG GCCAGAAGCC AGAGCTGCAG ATGCTGACTA TATCAGAGGA GCTGCCATTT
    GACAATGTGG ATGGCGGTGT ATGGAAGCAA GGCTTCGACA TCTCCTACAG CCCTCATGAC
    TGGGATGCGG AAGACCTGCA GGTGTTCGTG GTACCACACT CCCACAATGA TCCAGGCTGG
    ATCAAGACCT TTGACAAGTA CTACACAGAG CAGACCCAGC ACATCCTCAA CAGCATGGTG
    TCCAAACTGC AGGAGGACCC CCGGCGGCGC TTCCTCTGGG CGGAAGTCTC CTTCTTTGCC
    AAGTGGTGGG AAAACATCAA TGCCCAAAAG AAAGCAGCCG TCCGAAGGCT GGTGGGAAAT
    GGGCAGCTGG AGATTGTGAC AGGAGGCTGG GTGATGCCAG ACGAGGCCAA TTCCCATTAC
    TTTGCGCTGA TTGACCAGCT CATCGAGGGA CACCAGTGGC TGGAGAAAAA CCTTGGTGCA
    ACCCCACGCT CTGGCTGGGC TGTGGACCCC TTCGGATACA GCCCCACCAT CCCTTACCTG
    CTGCGCCGTG CCAACCTGAC CAGCATGCTG ATTCAGAGGG TGCATTATGC CATCAAGAAG
    CACTTTGCTT CCACCCACAG TCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCAGACTCT
    AGCACAGACA TCTTCTGCCA CATGATGCCC TTCTACAGCT ATGATGTGCC CCATACCTGC
    GGCCCAGACC CCAAGATCTG CTGCCAGTTT GATTTCAAAC GCTTGCCTGG TGGACGCATC
    AACTGCCCCT GGAAGGTGCC ACCTCGGGCG ATCACAGAGG CAAACGTGGC TGAGAGGGCT
    GCGCTGCTCC TGGACCAGTA TCGCAAGAAG TCCCGGCTCT TCCGAAGTAA CGTCCTCCTG
    GTGCCTCTGG GGGACGACTT CCGATATGAC AAGCCCCAGG AGTGGGATGC CCAGTTCTTT
    AACTACCAGC GGCTCTTTGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG
    TTTGGTACCC TCTCTGACTA TTTCGACGCC CTGTACAAGA AGACAGGGGT AGAACCAGGG
    GCCCGGCCTC CAGGGTTCCC TGTGCTAAGT GGGGACTTCT TCTCCTATGC GGACCGGGAG
    GACCATTACT GGACAGGCTA CTACACTTCA CGGCCCTTTT ACAAGAGCTT GGACCGGGTC
    CTGGAAGCCC ACCTGCGGGG GGCAGAGATT CTGTACAGCC TGGCTGTAGC TCACGCCCGC
    CGCTCTGGAC TGGCTAGCCA ATACCCACTC TCTAACTTCG CCCTTCTGAC GGAAGCTCGG
    CGCACCCTGG GCCTCTTCCA GCACCACGAT GCCATCACCG GCACCGCCAA AGAGGCAGTT
    GTGGCCGACT ATGGCGTCAG GCTTCTGCGC TCCCTTGTCA ACCTGAAGCA AGTAATCATC
    AATGCTGCTC ACTATCTGGT GTTGGGGGAC AAGGAGACCT ACCACTTCGA CCCTGAGGCA
    CCCTTCCTCC AAATGGATGA AACCCGCTTA AATCACGACA CACTGCCAGA GCGCACAGTG
    ATCCAGCTAG ATTCCTCACC CAGGTATGTG GTGCTCTTCA ACCCGCTGGA ACAGGAGCGG
    CTGAGTGTGG TGTCCCTGTT GGTCAGCTCG CCCCGGGTGC GGGTCCTTTC AGAGGAGGGC
    CAGCCCCTGG CTGTGCAGAT CAGTGCACAC TGGAGCTCTG CCACCGACAT GGTCTCTGAT
    GTCTGCCAGG TATCTATGCC CATCCGCCTG CCGGCCCTGG GCCTCAGCGT GCTACAGCTG
    CAGCTGGGCC TCGATGGGCA CCGCACCCTG CCCTCCTCTG TGCGTGTCTA CCTGCATGGC
    CGTCAGCTGG CCGTCAGCAG GCAGGACGCT TTCCCACTGC GCATCATTGA CTCTGGCACC
    AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC
    CTCAAGAGCA TCCGAAGGGT GGACGAGGAG CAGGAGCGGC GGGTGGACAT GGAGTTCCTC
    ATCTATGGCA CCCGCGCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACAGG
    GAGGCCCAGC CCTATATCCC CAAGGACCCC CCCGTGCTGC GTGTCACCGA AGGCCCTTTC
    TTCTCGGAGG TGGTGGCATA CTACGAGCAT ATTCGCCAGG TGGTCCGGCT ATATAATCTG
    CCAGGGGTGG AGGGGCTGTC TCTGGACATG TCGTCCTTGG TGGACATCCG GGATTACATC
    AACAAGGAGC TGGCCCTGCG CATCCACACA GACATTGACA GCCAGGGTGC CTTCTTCACG
    GACCTCAATG GCTTTCAGGT GCAGCCCCGG CGCTATCTGA AGAAGCTGCC TCTGCAGGCC
    AATTTCTACC CCATGCCAGT CATGGCCTAC ATCCAGGATG CGCAGAACCG CCTCACACTG
    CACACTGCCC AGGCCCTGGG TGTCTCCAGT CTGCACGATG GCCAGCTGGA GGTGATCCTG
    GACCGACGGC TCATGCAAGA TGACAACCGG GGTCTGGGCC AAGGGCTCAA GGACAATAAA
    AGAACCTGCA ACCATTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG TGAGGTCCAG
    GATGGCCACA CCAGCAGCTA CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAT
    ATCCCAGTGT TTGCCCTGCC TGTGGCCAAG AGGCAGCTCC CCGGCCCTGG TCTACGCTCC
    TTTCATCCCC TGGCCTCCTC CCTGCCCTGT GACTTCCACC TGCTCAACCT GCGCACCCTG
    CAGGCCGAGG ATGATGCCTT GCCATCAGCA GAGACCGCAC TCATCTTACA CCGCAAGGGT
    TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCAGCACAAG CCAAGGCAAG
    GCAGCCCTGG GGAGCCTCTT CCATGGCCTG GATGTAGGTT TCCTGCAGCC GACCTCACTG
    ACATTACTGT ACCCACTGGC CGCACCCTCC AACAGCACTG ATGTCTACCT GGAGCCCATG
    GAGATTGCCA CCTTTCGCCT CCGCTTGGGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.