ADGRB2 cDNA ORF clone, Canis lupus familiaris(dog)

The following ADGRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe286875 XM_022412323.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe286872 XM_022412304.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe286873 XM_022412309.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe286874 XM_022412311.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35431 XM_014109915.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35432 XM_014109922.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35433 XM_014109926.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35434 XM_022412294.1
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35434 XM_014109928.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35435 XM_014109934.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03420 XM_005617658.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03420 XM_005617663.3
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03421 XM_005617659.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03422 XM_005617660.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03423 XM_005617661.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03424 XM_005617662.2
Latest version!
Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe35432 XM_014109922.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03424 XM_005617662.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe03420 XM_005617658.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe35431 XM_014109915.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03421 XM_005617659.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe03420 XM_005617663.2 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe35434 XM_014109928.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe35435 XM_014109934.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03423 XM_005617661.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe35433 XM_014109926.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe03422 XM_005617660.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe286875
    Clone ID Related Accession (Same CDS sequence) XM_022412323.1
    Accession Version XM_022412323.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGACT TTGAAAAAGA TGTGGATCTG GCTTGTCAGA
    CAGTTCTGTT CAAGGAGGTC AACACTTGCA ACCCATCTAC CATCACGGGC ACACTGTCCC
    GCCTATCCCT GGACGAGGAC GAGGAGCCCA AGTCCTGCCT CGTGGGTCCT GAGGGCGGCC
    TCAGCTTCTC ACCGCTGCCT GGGAATATCC TGGTGCCCAT GGCAGCCTCG CCAGGGCTGG
    GGGAGCCACC GCCCCCACAG GAGGCCAACC CTGTGTACAT GTGTGGGGAA GGTGGCCTCC
    GGCAGCTGGA CCTCACATGG CTGCGGCCCG AGCCGGGCTC TGAGGGGGAC TACATGGTGC
    TGCCCCGGCG GACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268031.1
    CDS211..4248
    Translation

    Target ORF information:

    RefSeq Version XM_022412323.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412323.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGACT TTGAAAAAGA TGTGGATCTG GCTTGTCAGA
    CAGTTCTGTT CAAGGAGGTC AACACTTGCA ACCCATCTAC CATCACGGGC ACACTGTCCC
    GCCTATCCCT GGACGAGGAC GAGGAGCCCA AGTCCTGCCT CGTGGGTCCT GAGGGCGGCC
    TCAGCTTCTC ACCGCTGCCT GGGAATATCC TGGTGCCCAT GGCAGCCTCG CCAGGGCTGG
    GGGAGCCACC GCCCCCACAG GAGGCCAACC CTGTGTACAT GTGTGGGGAA GGTGGCCTCC
    GGCAGCTGGA CCTCACATGG CTGCGGCCCG AGCCGGGCTC TGAGGGGGAC TACATGGTGC
    TGCCCCGGCG GACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286872
    Clone ID Related Accession (Same CDS sequence) XM_022412304.1
    Accession Version XM_022412304.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268012.1
    CDS211..4872
    Translation

    Target ORF information:

    RefSeq Version XM_022412304.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412304.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286873
    Clone ID Related Accession (Same CDS sequence) XM_022412309.1
    Accession Version XM_022412309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGGAGAAGC CCAGCCCTGG GGAGCATCCT GGCTTGTCCC AGCAGCGGAG ACATCAGAGC
    TGGAGCACCT TCAAGTCTAT GACATTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG
    GCCCTGCACC GAGCAGCAGC CTGGGAGCCC ACAGAACCAC CTGATGGTGA CTTCCAGACA
    GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268017.1
    CDS211..4839
    Translation

    Target ORF information:

    RefSeq Version XM_022412309.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGGAGAAGC CCAGCCCTGG GGAGCATCCT GGCTTGTCCC AGCAGCGGAG ACATCAGAGC
    TGGAGCACCT TCAAGTCTAT GACATTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG
    GCCCTGCACC GAGCAGCAGC CTGGGAGCCC ACAGAACCAC CTGATGGTGA CTTCCAGACA
    GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286874
    Clone ID Related Accession (Same CDS sequence) XM_022412311.1
    Accession Version XM_022412311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268019.1
    CDS211..4836
    Translation

    Target ORF information:

    RefSeq Version XM_022412311.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35431
    Clone ID Related Accession (Same CDS sequence) XM_014109915.1 , XM_014109915.2
    Accession Version XM_014109915.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965390.1
    CDS213..4898
    Translation

    Target ORF information:

    RefSeq Version XM_014109915.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35431
    Clone ID Related Accession (Same CDS sequence) XM_014109915.1 , XM_014109915.2
    Accession Version XM_014109915.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965390.1
    CDS211..4896
    Translation

    Target ORF information:

    RefSeq Version XM_014109915.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109915.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC
    TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC
    AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC
    GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG
    AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC
    CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG
    GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35432
    Clone ID Related Accession (Same CDS sequence) XM_014109922.2 , XM_014109922.1
    Accession Version XM_014109922.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965397.1
    CDS211..4893
    Translation

    Target ORF information:

    RefSeq Version XM_014109922.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35432
    Clone ID Related Accession (Same CDS sequence) XM_014109922.2 , XM_014109922.1
    Accession Version XM_014109922.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965397.1
    CDS213..4895
    Translation

    Target ORF information:

    RefSeq Version XM_014109922.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109922.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35433
    Clone ID Related Accession (Same CDS sequence) XM_014109926.2 , XM_014109926.1
    Accession Version XM_014109926.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965401.1
    CDS211..4767
    Translation

    Target ORF information:

    RefSeq Version XM_014109926.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109926.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35433
    Clone ID Related Accession (Same CDS sequence) XM_014109926.2 , XM_014109926.1
    Accession Version XM_014109926.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965401.1
    CDS213..4769
    Translation

    Target ORF information:

    RefSeq Version XM_014109926.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109926.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35434
    Clone ID Related Accession (Same CDS sequence) XM_022412294.1 , XM_014109928.2 , XM_014109928.1
    Accession Version XM_022412294.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268002.1
    CDS211..4929
    Translation

    Target ORF information:

    RefSeq Version XM_022412294.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35434
    Clone ID Related Accession (Same CDS sequence) XM_022412294.1 , XM_014109928.2 , XM_014109928.1
    Accession Version XM_014109928.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965403.1
    CDS35..4753
    Translation

    Target ORF information:

    RefSeq Version XM_014109928.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109928.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35434
    Clone ID Related Accession (Same CDS sequence) XM_022412294.1 , XM_014109928.2 , XM_014109928.1
    Accession Version XM_014109928.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965403.1
    CDS153..4871
    Translation

    Target ORF information:

    RefSeq Version XM_014109928.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109928.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC
    ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC
    TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT
    GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC
    TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC
    ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35435
    Clone ID Related Accession (Same CDS sequence) XM_014109934.1 , XM_014109934.2
    Accession Version XM_014109934.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC
    GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG
    GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG
    CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC
    TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC
    CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965409.1
    CDS153..4772
    Translation

    Target ORF information:

    RefSeq Version XM_014109934.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC
    GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG
    GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG
    CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC
    TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC
    CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35435
    Clone ID Related Accession (Same CDS sequence) XM_014109934.1 , XM_014109934.2
    Accession Version XM_014109934.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC
    GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG
    GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG
    CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC
    TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC
    CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965409.1
    CDS35..4654
    Translation

    Target ORF information:

    RefSeq Version XM_014109934.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014109934.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC
    GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG
    GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG
    CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC
    TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC
    CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03420
    Clone ID Related Accession (Same CDS sequence) XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
    Accession Version XM_005617658.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617715.1
    CDS213..4934
    Translation

    Target ORF information:

    RefSeq Version XM_005617658.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03420
    Clone ID Related Accession (Same CDS sequence) XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
    Accession Version XM_005617658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617715.1
    CDS211..4932
    Translation

    Target ORF information:

    RefSeq Version XM_005617658.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03420
    Clone ID Related Accession (Same CDS sequence) XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
    Accession Version XM_005617663.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617720.1
    CDS35..4756
    Translation

    Target ORF information:

    RefSeq Version XM_005617663.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03420
    Clone ID Related Accession (Same CDS sequence) XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
    Accession Version XM_005617663.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617720.1
    CDS152..4873
    Translation

    Target ORF information:

    RefSeq Version XM_005617663.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG
    TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT
    CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG
    GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG
    CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03421
    Clone ID Related Accession (Same CDS sequence) XM_005617659.2 , XM_005617659.1
    Accession Version XM_005617659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG
    CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA
    TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG
    GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617716.1
    CDS211..4875
    Translation

    Target ORF information:

    RefSeq Version XM_005617659.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG
    CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA
    TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG
    GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03421
    Clone ID Related Accession (Same CDS sequence) XM_005617659.2 , XM_005617659.1
    Accession Version XM_005617659.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG
    CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA
    TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG
    GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617716.1
    CDS213..4877
    Translation

    Target ORF information:

    RefSeq Version XM_005617659.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC
    TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG
    GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC
    GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC
    TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG
    TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC
    GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG
    CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC
    CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG
    GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT
    CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG
    GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT
    GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC
    CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT
    ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG
    AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC
    CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG
    CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA
    TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG
    GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03422
    Clone ID Related Accession (Same CDS sequence) XM_005617660.1 , XM_005617660.2
    Accession Version XM_005617660.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617717.1
    CDS213..4835
    Translation

    Target ORF information:

    RefSeq Version XM_005617660.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617660.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03422
    Clone ID Related Accession (Same CDS sequence) XM_005617660.1 , XM_005617660.2
    Accession Version XM_005617660.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617717.1
    CDS211..4833
    Translation

    Target ORF information:

    RefSeq Version XM_005617660.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617660.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT
    GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG
    ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC
    CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC
    TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG
    CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT
    ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC
    ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC
    TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG
    GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC
    ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC
    GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG
    ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC
    ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT
    GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC
    CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT
    GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC
    AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG
    GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG
    GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG
    CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC
    CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC
    GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG
    CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG
    AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG
    CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC
    TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC
    AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG
    CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT
    CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC
    GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG
    TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC
    CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC
    GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC
    TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG
    AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT
    TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC
    CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC
    GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC
    CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC
    ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG
    TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG
    GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG
    GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC
    CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG
    AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA
    AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA
    TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC
    TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG
    CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG
    TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG
    GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT
    GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT
    GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC
    CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA
    CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG
    CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA
    TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC
    CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG
    AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG
    AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG
    CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03423
    Clone ID Related Accession (Same CDS sequence) XM_005617661.2 , XM_005617661.1
    Accession Version XM_005617661.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG
    GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG
    ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG
    ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617718.1
    CDS211..4767
    Translation

    Target ORF information:

    RefSeq Version XM_005617661.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617661.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG
    GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG
    ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG
    ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03423
    Clone ID Related Accession (Same CDS sequence) XM_005617661.2 , XM_005617661.1
    Accession Version XM_005617661.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG
    GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG
    ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG
    ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617718.1
    CDS213..4769
    Translation

    Target ORF information:

    RefSeq Version XM_005617661.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG
    GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG
    ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG
    ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC
    TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT
    GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC
    AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC
    TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG
    CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT
    CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG
    GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC
    CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC
    ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG
    CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC
    CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT
    GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG
    GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA
    TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT
    GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG
    CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC
    ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC
    CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG
    GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT
    CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC
    ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG
    CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC
    AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA
    GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC
    CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG
    GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG
    CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC
    ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC
    CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC
    TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC
    GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC
    GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC
    TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC
    ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT
    GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC
    CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG
    GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC
    TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC
    ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC
    GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG
    GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG
    TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT
    GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC
    GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG
    ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG
    CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA
    CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC
    ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA
    GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG
    CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG
    AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC
    AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA
    GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03424
    Clone ID Related Accession (Same CDS sequence) XM_005617662.2 , XM_005617662.1
    Accession Version XM_005617662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product adhesion G protein-coupled receptor B2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG
    CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC
    AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC
    ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT
    GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC
    CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC
    AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG
    ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA
    GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC
    AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC
    CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG
    CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT
    CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG
    CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT
    GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT
    CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG
    GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG
    CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC
    AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC
    CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC
    CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC
    CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG
    ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG
    CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA
    GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA
    GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC
    AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC
    GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC
    AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC
    CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC
    TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC
    TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG
    GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA
    TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG
    GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG
    CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG
    TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG
    CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC
    CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG
    GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG
    CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT
    ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC
    AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG
    CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT
    CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA
    CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC
    TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC
    CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG
    AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA
    GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT
    ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG
    TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG
    CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG
    ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617719.1
    CDS211..4602
    Translation

    Target ORF information:

    RefSeq Version XM_005617662.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG
    CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC
    AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC
    ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT
    GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC
    CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC
    AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG
    ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA
    GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC
    AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC
    CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG
    CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT
    CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG
    CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT
    GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT
    CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG
    GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG
    CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC
    AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC
    CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC
    CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC
    CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG
    ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG
    CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA
    GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA
    GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC
    AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC
    GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC
    AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC
    CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC
    TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC
    TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG
    GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA
    TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG
    GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG
    CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG
    TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG
    CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC
    CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG
    GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG
    CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT
    ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC
    AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG
    CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT
    CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA
    CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC
    TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC
    CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG
    AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA
    GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT
    ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG
    TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG
    CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG
    ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe03424
    Clone ID Related Accession (Same CDS sequence) XM_005617662.2 , XM_005617662.1
    Accession Version XM_005617662.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product adhesion G protein-coupled receptor B2 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG
    CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC
    AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC
    ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT
    GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC
    CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC
    AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG
    ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA
    GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC
    AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC
    CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG
    CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT
    CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG
    CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT
    GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT
    CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG
    GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG
    CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC
    AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC
    CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC
    CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC
    CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG
    ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG
    CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA
    GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA
    GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC
    AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC
    GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC
    AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC
    CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC
    TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC
    TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG
    GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA
    TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG
    GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG
    CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG
    TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG
    CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC
    CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG
    GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG
    CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT
    ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC
    AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG
    CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT
    CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA
    CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC
    TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC
    CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG
    AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA
    GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT
    ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG
    TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG
    CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG
    ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005617719.1
    CDS213..4604
    Translation

    Target ORF information:

    RefSeq Version XM_005617662.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005617662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT
    TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT
    GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC
    TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT
    AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG
    GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG
    CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT
    CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG
    CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG
    TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG
    TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG
    CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC
    AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC
    ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT
    GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC
    CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC
    AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG
    ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA
    GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC
    AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC
    CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG
    CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT
    CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG
    CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT
    GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT
    CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG
    GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG
    CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC
    AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC
    CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC
    CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC
    CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG
    ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG
    CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA
    GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA
    GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC
    AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC
    GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC
    AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC
    CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC
    TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC
    TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG
    GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA
    TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG
    GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG
    CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG
    TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG
    CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC
    CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG
    GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG
    CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT
    ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC
    AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG
    CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT
    CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA
    CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC
    TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC
    CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG
    AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA
    GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT
    ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG
    TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG
    CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG
    ACAGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.