ADGRB2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014109922.2	XP_013965397.1	adhesion G protein-coupled receptor B2 isoform X4
XM_022412304.1	XP_022268012.1	adhesion G protein-coupled receptor B2 isoform X6
XM_022412311.1	XP_022268019.1	adhesion G protein-coupled receptor B2 isoform X8
XM_005617661.2	XP_005617718.1	adhesion G protein-coupled receptor B2 isoform X11
XM_005617662.2	XP_005617719.1	adhesion G protein-coupled receptor B2 isoform X12
XM_014109915.1	XP_013965390.1	adhesion G protein-coupled receptor B2 isoform X2
XM_022412294.1	XP_022268002.1	adhesion G protein-coupled receptor B2 isoform X2
XM_005617659.2	XP_005617716.1	adhesion G protein-coupled receptor B2 isoform X5
XM_014109926.2	XP_013965401.1	adhesion G protein-coupled receptor B2 isoform X10
XM_014109928.2	XP_013965403.1	adhesion G protein-coupled receptor B2 isoform X2
XM_005617658.1	XP_005617715.1	adhesion G protein-coupled receptor B2 isoform X1
XM_014109922.1	XP_013965397.1	adhesion G protein-coupled receptor B2 isoform X3
XM_005617660.1	XP_005617717.1	adhesion G protein-coupled receptor B2 isoform X5
XM_005617662.1	XP_005617719.1	adhesion G protein-coupled receptor B2 isoform X8
XM_005617658.2	XP_005617715.1	adhesion G protein-coupled receptor B2 isoform X1
XM_005617663.3	XP_005617720.1	adhesion G protein-coupled receptor B2 isoform X1
XM_014109915.2	XP_013965390.1	adhesion G protein-coupled receptor B2 isoform X3
XM_022412309.1	XP_022268017.1	adhesion G protein-coupled receptor B2 isoform X7
XM_005617659.1	XP_005617716.1	adhesion G protein-coupled receptor B2 isoform X4
XM_005617663.2	XP_005617720.1	adhesion G protein-coupled receptor B2 isoform X1
XM_014109928.1	XP_013965403.1	adhesion G protein-coupled receptor B2 isoform X9
XM_014109934.1	XP_013965409.1	adhesion G protein-coupled receptor B2 isoform X10
XM_014109934.2	XP_013965409.1	adhesion G protein-coupled receptor B2 isoform X13
XM_022412323.1	XP_022268031.1	adhesion G protein-coupled receptor B2 isoform X14
XM_005617661.1	XP_005617718.1	adhesion G protein-coupled receptor B2 isoform X6
XM_014109926.1	XP_013965401.1	adhesion G protein-coupled receptor B2 isoform X7
XM_005617660.2	XP_005617717.1	adhesion G protein-coupled receptor B2 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe286875	XM_022412323.1 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe286872	XM_022412304.1 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe286873	XM_022412309.1 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe286874	XM_022412311.1 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35431	XM_014109915.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35432	XM_014109922.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35433	XM_014109926.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35434	XM_022412294.1 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35434	XM_014109928.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35435	XM_014109934.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe03420	XM_005617658.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03420	XM_005617663.3 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03421	XM_005617659.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03422	XM_005617660.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03423	XM_005617661.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03424	XM_005617662.2 Latest version!	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe35432	XM_014109922.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe03424	XM_005617662.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe03420	XM_005617658.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe35431	XM_014109915.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe03421	XM_005617659.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe03420	XM_005617663.2	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe35434	XM_014109928.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35435	XM_014109934.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe03423	XM_005617661.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe35433	XM_014109926.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe03422	XM_005617660.1	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ADGRB2 ( XM_022412323.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268031.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OCe286875
Clone ID Related Accession (Same CDS sequence)	XM_022412323.1
Accession Version	XM_022412323.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGACT TTGAAAAAGA TGTGGATCTG GCTTGTCAGA 
CAGTTCTGTT CAAGGAGGTC AACACTTGCA ACCCATCTAC CATCACGGGC ACACTGTCCC 
GCCTATCCCT GGACGAGGAC GAGGAGCCCA AGTCCTGCCT CGTGGGTCCT GAGGGCGGCC 
TCAGCTTCTC ACCGCTGCCT GGGAATATCC TGGTGCCCAT GGCAGCCTCG CCAGGGCTGG 
GGGAGCCACC GCCCCCACAG GAGGCCAACC CTGTGTACAT GTGTGGGGAA GGTGGCCTCC 
GGCAGCTGGA CCTCACATGG CTGCGGCCCG AGCCGGGCTC TGAGGGGGAC TACATGGTGC 
TGCCCCGGCG GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268031.1
CDS	211..4248
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILTLKKMWIWLVRQFCSRRSTLATHLPSRAHCPAYPWTRTRSPSPASWVLRAASASHRCLGISWCPWQPRQGWGSHRPHRRPTLCTCVGKVASGSWTSHGCGPSRALRGTTWCCPGGL

Target ORF information:

RefSeq Version	XM_022412323.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412323.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGACT TTGAAAAAGA TGTGGATCTG GCTTGTCAGA 
CAGTTCTGTT CAAGGAGGTC AACACTTGCA ACCCATCTAC CATCACGGGC ACACTGTCCC 
GCCTATCCCT GGACGAGGAC GAGGAGCCCA AGTCCTGCCT CGTGGGTCCT GAGGGCGGCC 
TCAGCTTCTC ACCGCTGCCT GGGAATATCC TGGTGCCCAT GGCAGCCTCG CCAGGGCTGG 
GGGAGCCACC GCCCCCACAG GAGGCCAACC CTGTGTACAT GTGTGGGGAA GGTGGCCTCC 
GGCAGCTGGA CCTCACATGG CTGCGGCCCG AGCCGGGCTC TGAGGGGGAC TACATGGTGC 
TGCCCCGGCG GACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_022412304.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268012.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe286872
Clone ID Related Accession (Same CDS sequence)	XM_022412304.1
Accession Version	XM_022412304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268012.1
CDS	211..4872
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_022412304.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412304.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_022412309.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268017.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe286873
Clone ID Related Accession (Same CDS sequence)	XM_022412309.1
Accession Version	XM_022412309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGGAGAAGC CCAGCCCTGG GGAGCATCCT GGCTTGTCCC AGCAGCGGAG ACATCAGAGC 
TGGAGCACCT TCAAGTCTAT GACATTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GAGCAGCAGC CTGGGAGCCC ACAGAACCAC CTGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268017.1
CDS	211..4839
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_022412309.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412309.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGGAGAAGC CCAGCCCTGG GGAGCATCCT GGCTTGTCCC AGCAGCGGAG ACATCAGAGC 
TGGAGCACCT TCAAGTCTAT GACATTGGGC TCGCTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GAGCAGCAGC CTGGGAGCCC ACAGAACCAC CTGATGGTGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_022412311.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268019.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

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CloneID	OCe286874
Clone ID Related Accession (Same CDS sequence)	XM_022412311.1
Accession Version	XM_022412311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268019.1
CDS	211..4836
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_022412311.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412311.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109915.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965390.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

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CloneID	OCe35431
Clone ID Related Accession (Same CDS sequence)	XM_014109915.1 , XM_014109915.2
Accession Version	XM_014109915.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965390.1
CDS	213..4898
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109915.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109915.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109915.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965390.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

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CloneID	OCe35431
Clone ID Related Accession (Same CDS sequence)	XM_014109915.1 , XM_014109915.2
Accession Version	XM_014109915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109915.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965390.1
CDS	211..4896
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109915.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109915.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGAAGGTG ATGCACACCC GGAAGCGGCA CTCAGAACTC 
TACCACGAGC TCAACCAGAA ATTCCACACT TTCGACCGCT ACCGCAGCCA GTCTACAGCC 
AAGAGGGAGA AGCGGTGGAG TGTGTCCTCG GGTGGGACAG CCGAACGGAA CATGTCTAGC 
GAGAAGCCCA GCCCTGGGGA GCATCCTGGC TTGTCCCAGC AGCGGAGACA TCAGAGCTGG 
AGCACCTTCA AGTCTATGAC ATTGGGCTCG CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGAG CAGCAGCCTG GGAGCCCACA GAACCACCTG ATGGTGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109922.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965397.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

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CloneID	OCe35432
Clone ID Related Accession (Same CDS sequence)	XM_014109922.2 , XM_014109922.1
Accession Version	XM_014109922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965397.1
CDS	211..4893
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109922.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109922.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109922.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965397.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35432
Clone ID Related Accession (Same CDS sequence)	XM_014109922.2 , XM_014109922.1
Accession Version	XM_014109922.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965397.1
CDS	213..4895
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109922.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109922.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109926.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965401.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

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CloneID	OCe35433
Clone ID Related Accession (Same CDS sequence)	XM_014109926.2 , XM_014109926.1
Accession Version	XM_014109926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965401.1
CDS	211..4767
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109926.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109926.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109926.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965401.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

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CloneID	OCe35433
Clone ID Related Accession (Same CDS sequence)	XM_014109926.2 , XM_014109926.1
Accession Version	XM_014109926.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965401.1
CDS	213..4769
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109926.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109926.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_022412294.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268002.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

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CloneID	OCe35434
Clone ID Related Accession (Same CDS sequence)	XM_022412294.1 , XM_014109928.2 , XM_014109928.1
Accession Version	XM_022412294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268002.1
CDS	211..4929
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_022412294.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412294.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109928.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965403.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe35434
Clone ID Related Accession (Same CDS sequence)	XM_022412294.1 , XM_014109928.2 , XM_014109928.1
Accession Version	XM_014109928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965403.1
CDS	35..4753
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109928.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109928.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109928.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965403.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

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CloneID	OCe35434
Clone ID Related Accession (Same CDS sequence)	XM_022412294.1 , XM_014109928.2 , XM_014109928.1
Accession Version	XM_014109928.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965403.1
CDS	153..4871
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109928.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109928.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
GTGATGCACA CCCGGAAGCG GCACTCAGAA CTCTACCACG AGCTCAACCA GAAATTCCAC 
ACTTTCGACC GCTACCGCAG CCAGTCTACA GCCAAGAGGG AGAAGCGGTG GAGTGTGTCC 
TCGGGTGGGA CAGCCGAACG GAACATGTCT AGCGAGAAGC CCAGCCCTGG GGAGCATCCT 
GGCTTGTCCC AGCAGCGGAG ACATCAGAGC TGGAGCACCT TCAAGTCTAT GACATTGGGC 
TCGCTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GAGCAGCAGC CTGGGAGCCC 
ACAGAACCAC CTGATGGTGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109934.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965409.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

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CloneID	OCe35435
Clone ID Related Accession (Same CDS sequence)	XM_014109934.1 , XM_014109934.2
Accession Version	XM_014109934.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC 
GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG 
GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG 
CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC 
TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC 
CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965409.1
CDS	153..4772
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109934.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109934.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC 
GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG 
GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG 
CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC 
TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC 
CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_014109934.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013965409.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.

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CloneID	OCe35435
Clone ID Related Accession (Same CDS sequence)	XM_014109934.1 , XM_014109934.2
Accession Version	XM_014109934.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014109934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC 
GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG 
GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG 
CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC 
TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC 
CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013965409.1
CDS	35..4654
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_014109934.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109934.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GGTGATGCAC ACCCGGAAGC GGCACTCAGA ACTCTACCAC 
GAGCTCAACC AGAAATTCCA CACTTTCGAC CGCTACCGCA GCCAGTCTAC AGCCAAGAGG 
GAGAAGCGGT GGAGTGTGTC CTCGGGTGGG ACAGCCGAAC GGAACATGTC TAGCGAGAAG 
CCCAGCCCTG GGGAGCATCC TGGCTTGTCC CAGCAGCGGA GACATCAGAG CTGGAGCACC 
TTCAAGTCTA TGACATTGGG CTCGCTGCCC CCCAAGCCCC GAGAACGGCT GGCCCTGCAC 
CGAGCAGCAG CCTGGGAGCC CACAGAACCA CCTGATGGTG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617658.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617715.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

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CloneID	OCe03420
Clone ID Related Accession (Same CDS sequence)	XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
Accession Version	XM_005617658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617715.1
CDS	213..4934
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617658.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617658.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617658.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617715.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03420
Clone ID Related Accession (Same CDS sequence)	XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
Accession Version	XM_005617658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617715.1
CDS	211..4932
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617658.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617658.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617663.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617720.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe03420
Clone ID Related Accession (Same CDS sequence)	XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
Accession Version	XM_005617663.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617720.1
CDS	35..4756
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617663.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617663.3

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617663.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617720.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

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CloneID	OCe03420
Clone ID Related Accession (Same CDS sequence)	XM_005617658.1 , XM_005617658.2 , XM_005617663.3 , XM_005617663.2
Accession Version	XM_005617663.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005617663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617720.1
CDS	152..4873
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617663.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617663.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG 
TCCTCGGGTG GGACAGCCGA ACGGAACATG TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT 
CCTGGCTTGT CCCAGCAGCG GAGACATCAG AGCTGGAGCA CCTTCAAGTC TATGACATTG 
GGCTCGCTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG 
CCCACAGAAC CACCTGATGG TGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617659.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617716.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

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CloneID	OCe03421
Clone ID Related Accession (Same CDS sequence)	XM_005617659.2 , XM_005617659.1
Accession Version	XM_005617659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG 
CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA 
TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG 
GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617716.1
CDS	211..4875
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617659.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617659.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG 
CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA 
TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG 
GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617659.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617716.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

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CloneID	OCe03421
Clone ID Related Accession (Same CDS sequence)	XM_005617659.2 , XM_005617659.1
Accession Version	XM_005617659.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG 
CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA 
TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG 
GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617716.1
CDS	213..4877
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617659.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617659.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC 
TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG CTCAGCTCAG CCTCGGCCAG GAACGCCATG 
GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCGGCC 
GTCCTGGCCA TGACAGACCG GCGCTCCGTC CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC 
TCGGCGCAGG GCTTTGTCAT CACTGCTGTG CACTGCTTCC TGCGCCGAGA GGTCCAGGAC 
GTGGTGAAGT GCCAGATGGG TGTATGCCGG GCCGATGAGA GTGAAGACTC CCCCGACTCA 
TGTAAAAATG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT 
CAGACAGTTC TGTTCAAGGA GGTCAACACT TGCAACCCAT CTACCATCAC GGGCACACTG 
TCCCGCCTAT CCCTGGACGA GGACGAGGAG CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC 
GGCCTCAGCT TCTCACCGCT GCCTGGGAAT ATCCTGGTGC CCATGGCAGC CTCGCCAGGG 
CTGGGGGAGC CACCGCCCCC ACAGGAGGCC AACCCTGTGT ACATGTGTGG GGAAGGTGGC 
CTCCGGCAGC TGGACCTCAC ATGGCTGCGG CCCGAGCCGG GCTCTGAGGG GGACTACATG 
GTGCTGCCCC GGCGGACTCT GAGCCTGCAG CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT 
CCCCCAAGGG CCCGGCCTGA GGGCACCCCT CGGAGGGCTG CCAAGACACT GGCCCACCCG 
GAAGGCTACC CCAGCTTCCT GTCTGTGGAA CACTCAGGCC TGGGGCTGGG CCCTGCCTAT 
GGGTCTCTAC AGAACCCCTA TGGGATGACC TTCCAGCCAC CACCACCGAC GCCCAGTGCC 
CGCCAAGTAT CTGAGCCAGG GGAACGCAGC CGGACCATGC CCCGCACTGT GCCAGGCTCT 
ACCATGAAGC TGGGCTCCCT GGAGCGAAAG AAGTTACGAT ATTCAGACCT GGACTTTGAG 
AAGGTGATGC ACACCCGGAA GCGGCACTCA GAACTCTACC ACGAGCTCAA CCAGAAATTC 
CACACTTTCG ACCGCTACCG CAGCCAGTCT ACAGCCAAGG AGAAGCCCAG CCCTGGGGAG 
CATCCTGGCT TGTCCCAGCA GCGGAGACAT CAGAGCTGGA GCACCTTCAA GTCTATGACA 
TTGGGCTCGC TGCCCCCCAA GCCCCGAGAA CGGCTGGCCC TGCACCGAGC AGCAGCCTGG 
GAGCCCACAG AACCACCTGA TGGTGACTTC CAGACAGAGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617660.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617717.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

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CloneID	OCe03422
Clone ID Related Accession (Same CDS sequence)	XM_005617660.1 , XM_005617660.2
Accession Version	XM_005617660.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617717.1
CDS	213..4835
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617660.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617660.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617660.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617717.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe03422
Clone ID Related Accession (Same CDS sequence)	XM_005617660.1 , XM_005617660.2
Accession Version	XM_005617660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617660.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617717.1
CDS	211..4833
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617660.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617660.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGGCGA CCCGGCGGCT 
GAGGAGTGGT CCCCGTGGAG CGTGTGTTCC CTGACGTGTG GGCAGGGTCT GCAGGTGCGG 
ACCCGCTCCT GCGTGTCCTC CCCCTATGGG ACCCTGTGCA GCGGGCCCCT GCGGGAGACC 
CGGCCCTGCA ACAATTCAGC CACCTGCCCA GTGCACGGCG TGTGGGAGGA GTGGGGGTCC 
TGGAGCCTGT GCTCCCGCAG CTGCGGGCGG GGGTCCCGGA GCCGGATGCG GACCTGCGTG 
CCCCCCCAGC ACGGCGGCAA GGCCTGCGAG GGTCCCGAGC TGCAGACTAA GCTCTGCAGT 
ATGGCCGCTT GCCCGGTGGA AGGCCAGTGG CTAGAATGGG GTCCCTGGGG CCCATGCTCC 
ACATCCTGTG CCAATGGGAC CCAGCAGCGC AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC 
TGGGCCACGT GTACGGGTGC CCTCACGGAC ACACGCGAGT GCAGCAACCT TGAGTGCCCG 
GCCACAGATG GCAAGTGGGG GCCGTGGAAT GCATGGAGCC TGTGCTCCAA GACGTGTGAC 
ACAGGCTGGC AGCGCCGCTT CCGCATGTGC CAGGCCACGG GTGCGCAGGG CTACCCCTGC 
GAGGGTACCG GAGAGGAGGT GAAGCCTTGC AGTGAGAAGA GATGTCCAGC CTTCCATGAG 
ATGTGCAGGG ACGAGTATGT GATGCTGATG ACGTGGAAGA AGGCGGCTGC TGGCGAGATC 
ATCTATAACA AGTGCCCCCC CAACGCCTCA GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT 
GCCCAGGGCG TGGCATACTG GGGTCTGCCC AGCTTCGCCC GCTGCATCTC CCACGAGTAC 
CGCTACCTAT ACCTGTCCCT TCGGGAGCAC CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT 
GAGGGCATGT CACAAGTGGT GCGCAGCTTG CAAGAGCTAC TGGCCCGGCG CACTTACTAC 
AGTGGGGACC TGCTCTTCTC TGTGGACATT CTAAGGAACG TCACTGACAC CTTCAAGAGG 
GCCACCTATG TGCCCTCGGC TGATGATGTG CAGCGCTTCT TCCAGGTGGT GAGCTTCATG 
GTGGATGCAG AGAACAAGGA TAAGTGGGAT GATGCTCAGC AGGTATCCCC GGGCTCTGTG 
CACCTGCTTC GTGTCGTGGA GGACTTCATT CACCTGGTGG GAGATGCCCT CAAGGCCTTC 
CAGAGCTCTC TGATTGTCAC AGACAACCTG GTGATCAGCA TTCAGCGAGA ACCGGTCTCC 
GCCGTGTCCA GTGACATCAC GTTCCCCATG CGTGGCCGCA GGGGCATGAA GGACTGGGTG 
CGGCACTCGG AGGACCGCCT CTTCCTACCC AAGGAGGTGC TCAGCCTTTC CACCCCTGGG 
AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC CCTGGCAGGG GGAGGGGCCC AGGAACCGTG 
CCCCCTGGCC CAGGCCACTC CCACCAGCGC CTCCTGCCAG CAGATCCTGA GGAGTCGTCC 
TCCTACTTTG TGATCGGTGC TGTGCTTTAC CGCACGCTTG GCCTCATCCT GCCACCCCCC 
AGACCCCCGC TGGCCGTCAC GTCCAGAGTG ATGACAGTGA CTGTGCGGCC CCCCACCCAG 
CCACCAGCTG AACCTCTTAT CACAGTGGAG CTCTCCTACA TCATCAACGG CACCACGGAT 
CCCCACTGTG CCAGCTGGGA CTACTCCCGA GCAGATGCCA GCTCAGGGGA CTGGGACACC 
GAGAGCTGCC AGACGCTGGA GACACAGGCA GCCCACACTC GCTGCCAGTG CCAGCACCTG 
TCTACCTTTG CTGTGCTGGC CCAGCCACCC AAGGACCTGA CCCTGGAGCT GGCAGGCTCC 
CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC GTGTCGTGCA TGGCGCTGCT CACCCTCCTC 
GCCATCTATG CGGCCTTCTG GAGGTTCATC AAATCCGAGC GCTCCATCAT CTTGTTGAAC 
TTCTGCTTGT CCATCCTGGC GTCCAACGTC CTGATCCTCG TGGGCCAGTC ACGGGTGCTG 
AGCAAGGGTG TATGCACCAT GACTGCCGCC TTCTTGCACT TCTTCTTCCT CTCCTCCTTT 
TGCTGGGTGC TCACTGAGGC CTGGCAGTCC TACCTGGCTG TCATCGGACG GATGCGTACC 
CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC 
GTGTCTGTCG GCTTCACCCG CACCAAAGGA TACGGTACAT CCAGCTACTG CTGGCTCTCC 
CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG GGGCCCGCTG CTGTCATCGT CCTGGTGAAC 
ATGCTCATCG GAATCATCGT CTTTAACAAG CTCATGGCAC GCGATGGCAT CTCGGACAAG 
TCCAAGAAGC AGAGGGCCGG GGCCTCGCTC TGGAGCTCCT GCGTGGTGCT GCCCCTGCTG 
GCGCTCACCT GGATGTCGGC CGTCCTGGCC ATGACAGACC GGCGCTCCGT CCTCTTCCAG 
GCCCTCTTCG CTGTCTTCAA CTCGGCGCAG GGCTTTGTCA TCACTGCTGT GCACTGCTTC 
CTGCGCCGAG AGGTCCAGGA CGTGGTGAAG TGCCAGATGG GTGTATGCCG GGCCGATGAG 
AGTGAAGACT CCCCCGACTC ATGTAAAAAT GGGCAGCTGC AGATCCTGTC AGACTTTGAA 
AAAGATGTGG ATCTGGCTTG TCAGACAGTT CTGTTCAAGG AGGTCAACAC TTGCAACCCA 
TCTACCATCA CGGGCACACT GTCCCGCCTA TCCCTGGACG AGGACGAGGA GCCCAAGTCC 
TGCCTCGTGG GTCCTGAGGG CGGCCTCAGC TTCTCACCGC TGCCTGGGAA TATCCTGGTG 
CCCATGGCAG CCTCGCCAGG GCTGGGGGAG CCACCGCCCC CACAGGAGGC CAACCCTGTG 
TACATGTGTG GGGAAGGTGG CCTCCGGCAG CTGGACCTCA CATGGCTGCG GCCCGAGCCG 
GGCTCTGAGG GGGACTACAT GGTGCTGCCC CGGCGGACTC TGAGCCTGCA GCCTGGCAGT 
GGTGGCGGAG CTGGTGAAGA TCCCCCAAGG GCCCGGCCTG AGGGCACCCC TCGGAGGGCT 
GCCAAGACAC TGGCCCACCC GGAAGGCTAC CCCAGCTTCC TGTCTGTGGA ACACTCAGGC 
CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA CAGAACCCCT ATGGGATGAC CTTCCAGCCA 
CCACCACCGA CGCCCAGTGC CCGCCAAGTA TCTGAGCCAG GGGAACGCAG CCGGACCATG 
CCCCGCACTG TGCCAGGCTC TACCATGAAG CTGGGCTCCC TGGAGCGAAA GAAGTTACGA 
TATTCAGACC TGGACTTTGA GAAGGTGATG CACACCCGGA AGCGGCACTC AGAACTCTAC 
CACGAGCTCA ACCAGAAATT CCACACTTTC GACCGCTACC GCAGCCAGTC TACAGCCAAG 
AGGGAGAAGC GGTGGAGTGT GTCCTCGGGT GGGACAGCCG AACGGAACAT GTCTAGCGAG 
AAGCCCAGCC CTGGGGAGCA TCCTGGCTTG TCCCAGCAGC GGAGACATCA GAGCTGGAGC 
ACCTTCAAGT CTATGACATT GGGCTCGCTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGAGCAG CAGCCTGGGA GCCCACAGAA CCACCTGATG GTGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617661.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617718.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

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CloneID	OCe03423
Clone ID Related Accession (Same CDS sequence)	XM_005617661.2 , XM_005617661.1
Accession Version	XM_005617661.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG 
GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG 
ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG 
ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617718.1
CDS	211..4767
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617661.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617661.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG 
GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG 
ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG 
ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617661.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617718.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

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CloneID	OCe03423
Clone ID Related Accession (Same CDS sequence)	XM_005617661.2 , XM_005617661.1
Accession Version	XM_005617661.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG 
GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG 
ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG 
ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617718.1
CDS	213..4769
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617661.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617661.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGCA CGGCGTGTGG 
GAGGAGTGGG GGTCCTGGAG CCTGTGCTCC CGCAGCTGCG GGCGGGGGTC CCGGAGCCGG 
ATGCGGACCT GCGTGCCCCC CCAGCACGGC GGCAAGGCCT GCGAGGGTCC CGAGCTGCAG 
ACTAAGCTCT GCAGTATGGC CGCTTGCCCG GTGGAAGGCC AGTGGCTAGA ATGGGGTCCC 
TGGGGCCCAT GCTCCACATC CTGTGCCAAT GGGACCCAGC AGCGCAGCCG GAAGTGCAGT 
GTGGCGGGCC CAGCCTGGGC CACGTGTACG GGTGCCCTCA CGGACACACG CGAGTGCAGC 
AACCTTGAGT GCCCGGCCAC AGATGGCAAG TGGGGGCCGT GGAATGCATG GAGCCTGTGC 
TCCAAGACGT GTGACACAGG CTGGCAGCGC CGCTTCCGCA TGTGCCAGGC CACGGGTGCG 
CAGGGCTACC CCTGCGAGGG TACCGGAGAG GAGGTGAAGC CTTGCAGTGA GAAGAGATGT 
CCAGCCTTCC ATGAGATGTG CAGGGACGAG TATGTGATGC TGATGACGTG GAAGAAGGCG 
GCTGCTGGCG AGATCATCTA TAACAAGTGC CCCCCCAACG CCTCAGGGTC TGCCAGCCGC 
CGCTGTCTCC TCAGTGCCCA GGGCGTGGCA TACTGGGGTC TGCCCAGCTT CGCCCGCTGC 
ATCTCCCACG AGTACCGCTA CCTATACCTG TCCCTTCGGG AGCACCTGGC CAAAGGGCAG 
CGCATGCTGG CAGGTGAGGG CATGTCACAA GTGGTGCGCA GCTTGCAAGA GCTACTGGCC 
CGGCGCACTT ACTACAGTGG GGACCTGCTC TTCTCTGTGG ACATTCTAAG GAACGTCACT 
GACACCTTCA AGAGGGCCAC CTATGTGCCC TCGGCTGATG ATGTGCAGCG CTTCTTCCAG 
GTGGTGAGCT TCATGGTGGA TGCAGAGAAC AAGGATAAGT GGGATGATGC TCAGCAGGTA 
TCCCCGGGCT CTGTGCACCT GCTTCGTGTC GTGGAGGACT TCATTCACCT GGTGGGAGAT 
GCCCTCAAGG CCTTCCAGAG CTCTCTGATT GTCACAGACA ACCTGGTGAT CAGCATTCAG 
CGAGAACCGG TCTCCGCCGT GTCCAGTGAC ATCACGTTCC CCATGCGTGG CCGCAGGGGC 
ATGAAGGACT GGGTGCGGCA CTCGGAGGAC CGCCTCTTCC TACCCAAGGA GGTGCTCAGC 
CTTTCCACCC CTGGGAAGCC AGCTGCCTCT GGCACCTCGG GCAGCCCTGG CAGGGGGAGG 
GGCCCAGGAA CCGTGCCCCC TGGCCCAGGC CACTCCCACC AGCGCCTCCT GCCAGCAGAT 
CCTGAGGAGT CGTCCTCCTA CTTTGTGATC GGTGCTGTGC TTTACCGCAC GCTTGGCCTC 
ATCCTGCCAC CCCCCAGACC CCCGCTGGCC GTCACGTCCA GAGTGATGAC AGTGACTGTG 
CGGCCCCCCA CCCAGCCACC AGCTGAACCT CTTATCACAG TGGAGCTCTC CTACATCATC 
AACGGCACCA CGGATCCCCA CTGTGCCAGC TGGGACTACT CCCGAGCAGA TGCCAGCTCA 
GGGGACTGGG ACACCGAGAG CTGCCAGACG CTGGAGACAC AGGCAGCCCA CACTCGCTGC 
CAGTGCCAGC ACCTGTCTAC CTTTGCTGTG CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GCTCCCCCTC GGTCCCCCTC GTGATCGGCT GTGCCGTGTC GTGCATGGCG 
CTGCTCACCC TCCTCGCCAT CTATGCGGCC TTCTGGAGGT TCATCAAATC CGAGCGCTCC 
ATCATCTTGT TGAACTTCTG CTTGTCCATC CTGGCGTCCA ACGTCCTGAT CCTCGTGGGC 
CAGTCACGGG TGCTGAGCAA GGGTGTATGC ACCATGACTG CCGCCTTCTT GCACTTCTTC 
TTCCTCTCCT CCTTTTGCTG GGTGCTCACT GAGGCCTGGC AGTCCTACCT GGCTGTCATC 
GGACGGATGC GTACCCGCCT TGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTGCCC 
GCCCTGGTGG TGGCCGTGTC TGTCGGCTTC ACCCGCACCA AAGGATACGG TACATCCAGC 
TACTGCTGGC TCTCCCTGGA GGGTGGCCTG CTCTATGCTT TTGTGGGGCC CGCTGCTGTC 
ATCGTCCTGG TGAACATGCT CATCGGAATC ATCGTCTTTA ACAAGCTCAT GGCACGCGAT 
GGCATCTCGG ACAAGTCCAA GAAGCAGAGG GCCGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTCC CCTGCTCAGC GTGCGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAACG CCATGGCCTC GCTCTGGAGC TCCTGCGTGG TGCTGCCCCT GCTGGCGCTC 
ACCTGGATGT CGGCCGTCCT GGCCATGACA GACCGGCGCT CCGTCCTCTT CCAGGCCCTC 
TTCGCTGTCT TCAACTCGGC GCAGGGCTTT GTCATCACTG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTAT GCCGGGCCGA TGAGAGTGAA 
GACTCCCCCG ACTCATGTAA AAATGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCTACC 
ATCACGGGCA CACTGTCCCG CCTATCCCTG GACGAGGACG AGGAGCCCAA GTCCTGCCTC 
GTGGGTCCTG AGGGCGGCCT CAGCTTCTCA CCGCTGCCTG GGAATATCCT GGTGCCCATG 
GCAGCCTCGC CAGGGCTGGG GGAGCCACCG CCCCCACAGG AGGCCAACCC TGTGTACATG 
TGTGGGGAAG GTGGCCTCCG GCAGCTGGAC CTCACATGGC TGCGGCCCGA GCCGGGCTCT 
GAGGGGGACT ACATGGTGCT GCCCCGGCGG ACTCTGAGCC TGCAGCCTGG CAGTGGTGGC 
GGAGCTGGTG AAGATCCCCC AAGGGCCCGG CCTGAGGGCA CCCCTCGGAG GGCTGCCAAG 
ACACTGGCCC ACCCGGAAGG CTACCCCAGC TTCCTGTCTG TGGAACACTC AGGCCTGGGG 
CTGGGCCCTG CCTATGGGTC TCTACAGAAC CCCTATGGGA TGACCTTCCA GCCACCACCA 
CCGACGCCCA GTGCCCGCCA AGTATCTGAG CCAGGGGAAC GCAGCCGGAC CATGCCCCGC 
ACTGTGCCAG GCTCTACCAT GAAGCTGGGC TCCCTGGAGC GAAAGAAGTT ACGATATTCA 
GACCTGGACT TTGAGAAGGT GATGCACACC CGGAAGCGGC ACTCAGAACT CTACCACGAG 
CTCAACCAGA AATTCCACAC TTTCGACCGC TACCGCAGCC AGTCTACAGC CAAGAGGGAG 
AAGCGGTGGA GTGTGTCCTC GGGTGGGACA GCCGAACGGA ACATGTCTAG CGAGAAGCCC 
AGCCCTGGGG AGCATCCTGG CTTGTCCCAG CAGCGGAGAC ATCAGAGCTG GAGCACCTTC 
AAGTCTATGA CATTGGGCTC GCTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGA 
GCAGCAGCCT GGGAGCCCAC AGAACCACCT GATGGTGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617662.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617719.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.

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CloneID	OCe03424
Clone ID Related Accession (Same CDS sequence)	XM_005617662.2 , XM_005617662.1
Accession Version	XM_005617662.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	adhesion G protein-coupled receptor B2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG 
CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC 
AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC 
ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT 
GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC 
CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC 
AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG 
ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA 
GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC 
AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC 
CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG 
CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT 
CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG 
CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT 
GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT 
CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG 
GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG 
CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC 
AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC 
CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC 
CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC 
CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG 
ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG 
CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA 
GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA 
GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC 
GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC 
AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC 
CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC 
TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC 
TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA 
TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG 
GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG 
CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG 
CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC 
CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG 
GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT 
TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG 
CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT 
ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC 
AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG 
CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG 
CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT 
CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA 
CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC 
TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC 
CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG 
AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA 
GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT 
ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG 
TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG 
AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG 
CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG 
ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617719.1
CDS	211..4602
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617662.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617662.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG 
CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC 
AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC 
ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT 
GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC 
CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC 
AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG 
ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA 
GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC 
AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC 
CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG 
CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT 
CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG 
CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT 
GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT 
CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG 
GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG 
CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC 
AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC 
CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC 
CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC 
CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG 
ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG 
CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA 
GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA 
GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC 
GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC 
AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC 
CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC 
TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC 
TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA 
TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG 
GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG 
CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG 
CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC 
CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG 
GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT 
TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG 
CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT 
ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC 
AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG 
CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG 
CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT 
CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA 
CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC 
TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC 
CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG 
AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA 
GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT 
ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG 
TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG 
AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG 
CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG 
ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADGRB2 ( XM_005617662.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617719.1 Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe03424
Clone ID Related Accession (Same CDS sequence)	XM_005617662.2 , XM_005617662.1
Accession Version	XM_005617662.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	adhesion G protein-coupled receptor B2 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG 
CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC 
AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC 
ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT 
GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC 
CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC 
AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG 
ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA 
GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC 
AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC 
CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG 
CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT 
CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG 
CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT 
GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT 
CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG 
GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG 
CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC 
AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC 
CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC 
CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC 
CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG 
ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG 
CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA 
GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA 
GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC 
GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC 
AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC 
CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC 
TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC 
TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA 
TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG 
GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG 
CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG 
CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC 
CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG 
GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT 
TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG 
CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT 
ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC 
AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG 
CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG 
CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT 
CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA 
CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC 
TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC 
CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG 
AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA 
GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT 
ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG 
TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG 
AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG 
CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG 
ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617719.1
CDS	213..4604
Translation	MTPACPLLLSVILSLRLAAAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPSPEEAGAQAEAEAGRPEEEEEAAAAGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGAGPATTTPPGPPAAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSTSCANGTQQRSRKCSVAGPAWATCTGALTDTRECSNLECPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQATGAQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKKAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDAENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPVSAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSTPGKPAASGTSGSPGRGRGPGTVPPGPGHSHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADASSGDWDTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGSPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNVLILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGISDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQEANPVYMCGEGGLRQLDLTWLRPEPGSEGDYMVLPRRTLSLQPGSGGGAGEDPPRARPEGTPRRAAKTLAHPEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVSEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSTAKREKRWSVSSGGTAERNMSSEKPSPGEHPGLSQQRRHQSWSTFKSMTLGSLPPKPRERLALHRAAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_005617662.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617662.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
TTCGACCCTG CCCCCAGCGC CTGCTCCGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTT 
TCACTGCAGG ACCTCTTTCC CACCATCGCC TCAGGCTGCT CCTGGACCCT GGAGAACCCT 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGCACTCAC 
TTTGCCCCCC GTCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCT 
AGCCCAGAGG AGGCGGGGGC CCAGGCCGAG GCAGAGGCAG GGCGGCCAGA GGAGGAGGAG 
GAGGCGGCAG CGGCGGGGCT GGAACTGTGC GGCGGCTCGG GCCCCTTCAC CTTCCTGCAC 
TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCAGAGC CCTCAGAGGC CCCGCGCCTG 
CTGGCGCCGG CCGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC 
TCCAGCCAGT TCACCTGTGG CGTGCTCTGC CGCTGGAGCG AAGAGTGCGG CCGCGCTGCC 
GGCAGGGCCT GTGGCTTCGC CCAGCCCGGC TGCAGCTGCC CCGGGGAGGC GGGGGCTGGT 
CCCGCCACCA CCACGCCTCC AGGCCCTCCT GCTGCCCACA CTCTGTCCAA TGCCCTGGTG 
CCTGGGGGCC CGGCCCCACC TGCTGAGGCC GATTTGCATT CAGGGAGCAG CAATGACCTG 
TTTACGACTG AGATGAGATA TGGTGAGGAG CCGGAAGAGG AACCGAAGGT GAAAACCCAG 
TGGCCAAGGT CTGCAGATGA GCCTGGGCTG TACATGGCGC AGACAGTGGA AGGCCAGTGG 
CTAGAATGGG GTCCCTGGGG CCCATGCTCC ACATCCTGTG CCAATGGGAC CCAGCAGCGC 
AGCCGGAAGT GCAGTGTGGC GGGCCCAGCC TGGGCCACGT GTACGGGTGC CCTCACGGAC 
ACACGCGAGT GCAGCAACCT TGAGTGCCCG GCCACAGATG GCAAGTGGGG GCCGTGGAAT 
GCATGGAGCC TGTGCTCCAA GACGTGTGAC ACAGGCTGGC AGCGCCGCTT CCGCATGTGC 
CAGGCCACGG GTGCGCAGGG CTACCCCTGC GAGGGTACCG GAGAGGAGGT GAAGCCTTGC 
AGTGAGAAGA GATGTCCAGC CTTCCATGAG ATGTGCAGGG ACGAGTATGT GATGCTGATG 
ACGTGGAAGA AGGCGGCTGC TGGCGAGATC ATCTATAACA AGTGCCCCCC CAACGCCTCA 
GGGTCTGCCA GCCGCCGCTG TCTCCTCAGT GCCCAGGGCG TGGCATACTG GGGTCTGCCC 
AGCTTCGCCC GCTGCATCTC CCACGAGTAC CGCTACCTAT ACCTGTCCCT TCGGGAGCAC 
CTGGCCAAAG GGCAGCGCAT GCTGGCAGGT GAGGGCATGT CACAAGTGGT GCGCAGCTTG 
CAAGAGCTAC TGGCCCGGCG CACTTACTAC AGTGGGGACC TGCTCTTCTC TGTGGACATT 
CTAAGGAACG TCACTGACAC CTTCAAGAGG GCCACCTATG TGCCCTCGGC TGATGATGTG 
CAGCGCTTCT TCCAGGTGGT GAGCTTCATG GTGGATGCAG AGAACAAGGA TAAGTGGGAT 
GATGCTCAGC AGGTATCCCC GGGCTCTGTG CACCTGCTTC GTGTCGTGGA GGACTTCATT 
CACCTGGTGG GAGATGCCCT CAAGGCCTTC CAGAGCTCTC TGATTGTCAC AGACAACCTG 
GTGATCAGCA TTCAGCGAGA ACCGGTCTCC GCCGTGTCCA GTGACATCAC GTTCCCCATG 
CGTGGCCGCA GGGGCATGAA GGACTGGGTG CGGCACTCGG AGGACCGCCT CTTCCTACCC 
AAGGAGGTGC TCAGCCTTTC CACCCCTGGG AAGCCAGCTG CCTCTGGCAC CTCGGGCAGC 
CCTGGCAGGG GGAGGGGCCC AGGAACCGTG CCCCCTGGCC CAGGCCACTC CCACCAGCGC 
CTCCTGCCAG CAGATCCTGA GGAGTCGTCC TCCTACTTTG TGATCGGTGC TGTGCTTTAC 
CGCACGCTTG GCCTCATCCT GCCACCCCCC AGACCCCCGC TGGCCGTCAC GTCCAGAGTG 
ATGACAGTGA CTGTGCGGCC CCCCACCCAG CCACCAGCTG AACCTCTTAT CACAGTGGAG 
CTCTCCTACA TCATCAACGG CACCACGGAT CCCCACTGTG CCAGCTGGGA CTACTCCCGA 
GCAGATGCCA GCTCAGGGGA CTGGGACACC GAGAGCTGCC AGACGCTGGA GACACAGGCA 
GCCCACACTC GCTGCCAGTG CCAGCACCTG TCTACCTTTG CTGTGCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGCTCC CCCTCGGTCC CCCTCGTGAT CGGCTGTGCC 
GTGTCGTGCA TGGCGCTGCT CACCCTCCTC GCCATCTATG CGGCCTTCTG GAGGTTCATC 
AAATCCGAGC GCTCCATCAT CTTGTTGAAC TTCTGCTTGT CCATCCTGGC GTCCAACGTC 
CTGATCCTCG TGGGCCAGTC ACGGGTGCTG AGCAAGGGTG TATGCACCAT GACTGCCGCC 
TTCTTGCACT TCTTCTTCCT CTCCTCCTTT TGCTGGGTGC TCACTGAGGC CTGGCAGTCC 
TACCTGGCTG TCATCGGACG GATGCGTACC CGCCTTGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TGCCCGCCCT GGTGGTGGCC GTGTCTGTCG GCTTCACCCG CACCAAAGGA 
TACGGTACAT CCAGCTACTG CTGGCTCTCC CTGGAGGGTG GCCTGCTCTA TGCTTTTGTG 
GGGCCCGCTG CTGTCATCGT CCTGGTGAAC ATGCTCATCG GAATCATCGT CTTTAACAAG 
CTCATGGCAC GCGATGGCAT CTCGGACAAG TCCAAGAAGC AGAGGGCCGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTCCCCTGC TCAGCGTGCG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAACGCCATG GCCTCGCTCT GGAGCTCCTG CGTGGTGCTG 
CCCCTGCTGG CGCTCACCTG GATGTCGGCC GTCCTGGCCA TGACAGACCG GCGCTCCGTC 
CTCTTCCAGG CCCTCTTCGC TGTCTTCAAC TCGGCGCAGG GCTTTGTCAT CACTGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTATGCCGG 
GCCGATGAGA GTGAAGACTC CCCCGACTCA TGTAAAAATG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACT 
TGCAACCCAT CTACCATCAC GGGCACACTG TCCCGCCTAT CCCTGGACGA GGACGAGGAG 
CCCAAGTCCT GCCTCGTGGG TCCTGAGGGC GGCCTCAGCT TCTCACCGCT GCCTGGGAAT 
ATCCTGGTGC CCATGGCAGC CTCGCCAGGG CTGGGGGAGC CACCGCCCCC ACAGGAGGCC 
AACCCTGTGT ACATGTGTGG GGAAGGTGGC CTCCGGCAGC TGGACCTCAC ATGGCTGCGG 
CCCGAGCCGG GCTCTGAGGG GGACTACATG GTGCTGCCCC GGCGGACTCT GAGCCTGCAG 
CCTGGCAGTG GTGGCGGAGC TGGTGAAGAT CCCCCAAGGG CCCGGCCTGA GGGCACCCCT 
CGGAGGGCTG CCAAGACACT GGCCCACCCG GAAGGCTACC CCAGCTTCCT GTCTGTGGAA 
CACTCAGGCC TGGGGCTGGG CCCTGCCTAT GGGTCTCTAC AGAACCCCTA TGGGATGACC 
TTCCAGCCAC CACCACCGAC GCCCAGTGCC CGCCAAGTAT CTGAGCCAGG GGAACGCAGC 
CGGACCATGC CCCGCACTGT GCCAGGCTCT ACCATGAAGC TGGGCTCCCT GGAGCGAAAG 
AAGTTACGAT ATTCAGACCT GGACTTTGAG AAGGTGATGC ACACCCGGAA GCGGCACTCA 
GAACTCTACC ACGAGCTCAA CCAGAAATTC CACACTTTCG ACCGCTACCG CAGCCAGTCT 
ACAGCCAAGA GGGAGAAGCG GTGGAGTGTG TCCTCGGGTG GGACAGCCGA ACGGAACATG 
TCTAGCGAGA AGCCCAGCCC TGGGGAGCAT CCTGGCTTGT CCCAGCAGCG GAGACATCAG 
AGCTGGAGCA CCTTCAAGTC TATGACATTG GGCTCGCTGC CCCCCAAGCC CCGAGAACGG 
CTGGCCCTGC ACCGAGCAGC AGCCTGGGAG CCCACAGAAC CACCTGATGG TGACTTCCAG 
ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ADGRB2
Entrez Gene ID	487317
Full Name	adhesion G protein-coupled receptor B2
Synonyms	BAI2
General protein information	Preferred Names adhesion G protein-coupled receptor B2 Names adhesion G protein-coupled receptor B2 brain-specific angiogenesis inhibitor 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	BAI2	XP_001094702.2
Mus musculus (house mouse)	Bai2	NP_775094.2
Rattus norvegicus (Norway rat)	Bai2	NP_001101384.1
Homo sapiens (human)	BAI2	NP_001694.2
Pan troglodytes (chimpanzee)	BAI2	XP_003307988.1
Bos taurus (cattle)	BAI2	XP_003585838.2
Xenopus tropicalis (tropical clawed frog)	bai2	XP_002938534.2
Canis lupus familiaris (dog)	BAI2	XP_005617717.1
Gallus gallus (chicken)	BAI2	XP_423828.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ADGRB2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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