ADGRB2 cDNA ORF clone, Bos taurus(cattle)

The following ADGRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227867 XM_024982298.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa227868 XM_024982301.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa227860 XM_024982266.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227861 XM_024982268.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227862 XM_010802505.3
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227863 XM_024982274.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227864 XM_024982275.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227865 XM_024982281.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227866 XM_024982291.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227869 XM_024982306.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa46637 XM_010802505.2
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa89513 XM_015462437.1
Latest version!
Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227867
    Clone ID Related Accession (Same CDS sequence) XM_024982298.1
    Accession Version XM_024982298.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGGAA
    GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGTTCCA CGTCTTGTGC CAACGGGACC
    CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA GGCCCAGCCT GGGCCACGTG TGCCGGGGCC
    CTCACTGACA CCCGGGAGTG CGGCAACCTT GAGTGCCCGG CCACAGATGG CAAGTGGGGG
    CCATGGAATG CGTGGAGCCT GTGTTCCAAG ACATGTGACA CAGGCTGGCA GCGCCGTTTC
    CGCATGTGCC AGGCCACAGG CGCTCAGGGC TACCCCTGCG AGGGCACTGG CGAGGAGGTG
    AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA CGAGTACGTG
    ATGCTGATGA CATGGAAGAA GGCGGCTGCT GGCGAGATCA TCTACAACAA GTGCCCCCCC
    AATGCCTCAG GGTCTGCCAG CCGCCGCTGT CTGCTCAGTG CCCAGGGTGT GGCCTACTGG
    GGGCTGCCCA GCTTTGCCCG CTGCATCTCC CACGAGTACC GCTACCTGTA TCTGTCACTT
    CGGGAGCACC TGGCCAAGGG GCAGCGCATG CTGGCGGGCG AGGGCATGTC TCAGGTGGTG
    CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC ACTTACTACA GCGGGGACCT GCTTTTCTCC
    GTGGACATTC TGAGGAACGT CACCGACACC TTCAAGAGAG CCACCTACGT GCCCTCGGCT
    GATGACGTGC AGCGCTTCTT CCAGGTGGTG AGCTTCATGG TAGATGCAGA GAACAAGGAC
    AAGTGGGATG ACGCTCAGCA GGTGTCCCCA GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA
    GACTTCATTC ACCTGGTGGG CGATGCTCTC AAGGCCTTCC AGAGCTCTCT GATTGTCACA
    GATAACCTGG TGATCAGCAT TCAGCGAGAG CCTGTCTCCG CTGTTTCCAG CGACATCACG
    TTCCCCATGC GGGGCCGCCG GGGCATGAAG GACTGGGTGC GGTACTCAGA GGACCGCCTC
    TTCCTACCCA AGGAGGTGCT CAGCCTCTCC TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA
    GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC GGAACCGTGC CCCCTGGCCC CGGCCACTCC
    CACCAGCGCC TCCTGCCGGC AGACCCTGAG GATTCCTCCT CCTACTTTGT GATTGGTGCT
    GTGCTCTACC GCACCCTGGG CCTCATCCTG CCACCACCCA GGCCCCCGCT GGCTGTCACA
    TCCAGAGTGA TGACAGTGAC CGTAAGGCCT CCCACCCAGC CACTAGCTGA GCCCCTCATC
    ACAGTGGAGC TTTCCTACAT CATCAATGGC ACCACAGATC CCCATTGTGC CAGCTGGGAC
    TACTCCAGAG CAGACGCCAG CTCAGGGGAC TGGGATACCG AGAGCTGCCA GACGCTGGAG
    ACACAGGCAG CCCACACCCG CTGCCAGTGC CAGCGCCTGT CCACCTTCGC CGTGCTGGCC
    CAGCCGCCCA AGGACCTGAC CCTGGAGCTG GCGGGCGCCC CCTCGGTCCC CCTTGTGATC
    GGCTGTGCTG TGTCCTGCAT GGCACTGCTC ACCCTGCTTG CCATCTACGC CGCCTTCTGG
    AGGTTCATAA AATCTGAACG CTCCATCATC TTGCTGAACT TCTGCCTGTC CATCCTGGCA
    TCCAACATCC TCATCCTTGT GGGCCAGTCG CGGGTGCTGA GCAAGGGTGT GTGTACCATG
    ACCGCCGCCT TCCTGCACTT CTTCTTCCTG TCGTCCTTCT GCTGGGTGCT CACTGAGGCC
    TGGCAGTCCT ACCTGGCTGT CATCGGGCGG ATGCGCACCC GCCTTGTCCG CAAGCGCTTC
    CTCTGCCTGG GCTGGGGTGA GCAGGGCCCG TGCTGGACCC TGTCCCTGGG CCTCGGGGCG
    AGGGCAGCTG GAGAGCTTCA GCTTTGGCCC CTGGTCTGCT CTCTGGTGCC AGACGTGGCC
    CCGGCCCTGC TATCTGGGCA CAGGGGTCAG CTGGGCTGGG GGTCTGGGAG GATGAAGGAC
    CCCGAGGATG GGCGGCCATC TCACTCTGGC TCCCCCACTC CCCCAGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGAAGGTGA TGCACACCCG GAAACGGCAC
    TCGGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG
    TCCACGGCCA AGGAGAAGCC CAGTCCAGGG GAGCGTCCCG GCTTATCCCA ACAGCGGCGA
    CATCAGAGCT GGAGCACCTT CAAGTCTATG ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA
    GAGCGGCTGG CCCTGCACCG GGCAACAGCC TGGGAACCCA CAGAACCACC GGACGGCGAC
    TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838066.1
    CDS166..4623
    Translation

    Target ORF information:

    RefSeq Version XM_024982298.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982298.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGGAA
    GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGTTCCA CGTCTTGTGC CAACGGGACC
    CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA GGCCCAGCCT GGGCCACGTG TGCCGGGGCC
    CTCACTGACA CCCGGGAGTG CGGCAACCTT GAGTGCCCGG CCACAGATGG CAAGTGGGGG
    CCATGGAATG CGTGGAGCCT GTGTTCCAAG ACATGTGACA CAGGCTGGCA GCGCCGTTTC
    CGCATGTGCC AGGCCACAGG CGCTCAGGGC TACCCCTGCG AGGGCACTGG CGAGGAGGTG
    AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA CGAGTACGTG
    ATGCTGATGA CATGGAAGAA GGCGGCTGCT GGCGAGATCA TCTACAACAA GTGCCCCCCC
    AATGCCTCAG GGTCTGCCAG CCGCCGCTGT CTGCTCAGTG CCCAGGGTGT GGCCTACTGG
    GGGCTGCCCA GCTTTGCCCG CTGCATCTCC CACGAGTACC GCTACCTGTA TCTGTCACTT
    CGGGAGCACC TGGCCAAGGG GCAGCGCATG CTGGCGGGCG AGGGCATGTC TCAGGTGGTG
    CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC ACTTACTACA GCGGGGACCT GCTTTTCTCC
    GTGGACATTC TGAGGAACGT CACCGACACC TTCAAGAGAG CCACCTACGT GCCCTCGGCT
    GATGACGTGC AGCGCTTCTT CCAGGTGGTG AGCTTCATGG TAGATGCAGA GAACAAGGAC
    AAGTGGGATG ACGCTCAGCA GGTGTCCCCA GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA
    GACTTCATTC ACCTGGTGGG CGATGCTCTC AAGGCCTTCC AGAGCTCTCT GATTGTCACA
    GATAACCTGG TGATCAGCAT TCAGCGAGAG CCTGTCTCCG CTGTTTCCAG CGACATCACG
    TTCCCCATGC GGGGCCGCCG GGGCATGAAG GACTGGGTGC GGTACTCAGA GGACCGCCTC
    TTCCTACCCA AGGAGGTGCT CAGCCTCTCC TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA
    GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC GGAACCGTGC CCCCTGGCCC CGGCCACTCC
    CACCAGCGCC TCCTGCCGGC AGACCCTGAG GATTCCTCCT CCTACTTTGT GATTGGTGCT
    GTGCTCTACC GCACCCTGGG CCTCATCCTG CCACCACCCA GGCCCCCGCT GGCTGTCACA
    TCCAGAGTGA TGACAGTGAC CGTAAGGCCT CCCACCCAGC CACTAGCTGA GCCCCTCATC
    ACAGTGGAGC TTTCCTACAT CATCAATGGC ACCACAGATC CCCATTGTGC CAGCTGGGAC
    TACTCCAGAG CAGACGCCAG CTCAGGGGAC TGGGATACCG AGAGCTGCCA GACGCTGGAG
    ACACAGGCAG CCCACACCCG CTGCCAGTGC CAGCGCCTGT CCACCTTCGC CGTGCTGGCC
    CAGCCGCCCA AGGACCTGAC CCTGGAGCTG GCGGGCGCCC CCTCGGTCCC CCTTGTGATC
    GGCTGTGCTG TGTCCTGCAT GGCACTGCTC ACCCTGCTTG CCATCTACGC CGCCTTCTGG
    AGGTTCATAA AATCTGAACG CTCCATCATC TTGCTGAACT TCTGCCTGTC CATCCTGGCA
    TCCAACATCC TCATCCTTGT GGGCCAGTCG CGGGTGCTGA GCAAGGGTGT GTGTACCATG
    ACCGCCGCCT TCCTGCACTT CTTCTTCCTG TCGTCCTTCT GCTGGGTGCT CACTGAGGCC
    TGGCAGTCCT ACCTGGCTGT CATCGGGCGG ATGCGCACCC GCCTTGTCCG CAAGCGCTTC
    CTCTGCCTGG GCTGGGGTGA GCAGGGCCCG TGCTGGACCC TGTCCCTGGG CCTCGGGGCG
    AGGGCAGCTG GAGAGCTTCA GCTTTGGCCC CTGGTCTGCT CTCTGGTGCC AGACGTGGCC
    CCGGCCCTGC TATCTGGGCA CAGGGGTCAG CTGGGCTGGG GGTCTGGGAG GATGAAGGAC
    CCCGAGGATG GGCGGCCATC TCACTCTGGC TCCCCCACTC CCCCAGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGAAGGTGA TGCACACCCG GAAACGGCAC
    TCGGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG
    TCCACGGCCA AGGAGAAGCC CAGTCCAGGG GAGCGTCCCG GCTTATCCCA ACAGCGGCGA
    CATCAGAGCT GGAGCACCTT CAAGTCTATG ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA
    GAGCGGCTGG CCCTGCACCG GGCAACAGCC TGGGAACCCA CAGAACCACC GGACGGCGAC
    TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227868
    Clone ID Related Accession (Same CDS sequence) XM_024982301.1
    Accession Version XM_024982301.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC GTCGGCTTTA CCCGCACCAA AGGATACGGT
    ACATCCAGCT ACTGCTGGCT CTCTCTGGAG GGTGGCCTGC TTTATGCCTT CGTGGGCCCA
    GCAGCTGTAA TTGTCCTGGT GAATATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG
    GCTCGCGATG GCATCTCAGA CAAGTCCAAG AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC
    TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC ACCTGGATGT CTGCCGTCCT GGCCATGACA
    GACCGCCGCT CCGTCCTCTT CCAGGCCCTG TTCGCGGTCT TCAACTCTGC TCAGGGCTTT
    GTCATCACGG CCGTCCACTG CTTCCTGCGC CGAGAGGTCC AGGACGTGGT GAAGTGCCAG
    ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA GACTCCCCCG ACTCATGTAA GAACGGGCAG
    CTGCAGATCC TGTCAGACTT TGAAAAAGAT GTGGATCTGG CTTGTCAGAC TGTGCTGTTC
    AAGGAGGTCA ACACTTGCAA CCCATCCACC ATCACGGGCA CGCTGTCCCG CTTGTCTCTG
    GACGAGGATG AGGAGCCCAA GTCCTGCCTC GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA
    CCACTGCCTG GCAACATCCT GGTGCCCATG GCAGCCTCAC CAGGGCTGGG GGAGCCACCG
    CCCCCGCAGG AGGCCAACCC CGTGTACATG TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC
    CTCACATGGC TGCGGCCGGC CGAGCCGGGC TCCGAGGGGG ACTACATGGT GCTGCCCCGG
    AGGACTCTGA GTCTGCAGCC TGGCGGCGGG GGTGGAGGTG GCGAGGATCC CCCGCGGGCC
    CGGCCTGAGG GCACCCCCCG GCGGGCCTCC AAGTCCCTGG GCCACACCGA AGCCTACCCC
    AGCTTCCTGT CTGTGGAACA CTCGGGCCTG GGGCTGGGCC CTGCCTATGG GTCTCTACAG
    AACCCCTACG GGATGACCTT CCAACCGCCG CCGCCAACAC CCAGCGCCCG CCAGGTGCCA
    GAGCCGGGGG AGCGCAGCCG GACCATGCCC CGAACTGTGC CTGGCTCCAC CATGAAGCTG
    GGCTCCCTGG AGCGAAAGAA GTTACGGTAT TCAGACCTGG ACTTTGAGAA GGTGATGCAC
    ACCCGGAAAC GGCACTCGGA ACTCTACCAC GAGCTCAACC AGAAGTTCCA CACTTTCGAC
    CGCTACCGCA GCCAGTCCAC GGCCAAGGAG AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA
    TCCCAACAGC GGCGACATCA GAGCTGGAGC ACCTTCAAGT CTATGACGCT GGGCTCGCTG
    CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG CACCGGGCAA CAGCCTGGGA ACCCACAGAA
    CCACCGGACG GCGACTTCCA GACGGAGGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838069.1
    CDS166..4578
    Translation

    Target ORF information:

    RefSeq Version XM_024982301.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982301.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC GTCGGCTTTA CCCGCACCAA AGGATACGGT
    ACATCCAGCT ACTGCTGGCT CTCTCTGGAG GGTGGCCTGC TTTATGCCTT CGTGGGCCCA
    GCAGCTGTAA TTGTCCTGGT GAATATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG
    GCTCGCGATG GCATCTCAGA CAAGTCCAAG AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC
    TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC ACCTGGATGT CTGCCGTCCT GGCCATGACA
    GACCGCCGCT CCGTCCTCTT CCAGGCCCTG TTCGCGGTCT TCAACTCTGC TCAGGGCTTT
    GTCATCACGG CCGTCCACTG CTTCCTGCGC CGAGAGGTCC AGGACGTGGT GAAGTGCCAG
    ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA GACTCCCCCG ACTCATGTAA GAACGGGCAG
    CTGCAGATCC TGTCAGACTT TGAAAAAGAT GTGGATCTGG CTTGTCAGAC TGTGCTGTTC
    AAGGAGGTCA ACACTTGCAA CCCATCCACC ATCACGGGCA CGCTGTCCCG CTTGTCTCTG
    GACGAGGATG AGGAGCCCAA GTCCTGCCTC GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA
    CCACTGCCTG GCAACATCCT GGTGCCCATG GCAGCCTCAC CAGGGCTGGG GGAGCCACCG
    CCCCCGCAGG AGGCCAACCC CGTGTACATG TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC
    CTCACATGGC TGCGGCCGGC CGAGCCGGGC TCCGAGGGGG ACTACATGGT GCTGCCCCGG
    AGGACTCTGA GTCTGCAGCC TGGCGGCGGG GGTGGAGGTG GCGAGGATCC CCCGCGGGCC
    CGGCCTGAGG GCACCCCCCG GCGGGCCTCC AAGTCCCTGG GCCACACCGA AGCCTACCCC
    AGCTTCCTGT CTGTGGAACA CTCGGGCCTG GGGCTGGGCC CTGCCTATGG GTCTCTACAG
    AACCCCTACG GGATGACCTT CCAACCGCCG CCGCCAACAC CCAGCGCCCG CCAGGTGCCA
    GAGCCGGGGG AGCGCAGCCG GACCATGCCC CGAACTGTGC CTGGCTCCAC CATGAAGCTG
    GGCTCCCTGG AGCGAAAGAA GTTACGGTAT TCAGACCTGG ACTTTGAGAA GGTGATGCAC
    ACCCGGAAAC GGCACTCGGA ACTCTACCAC GAGCTCAACC AGAAGTTCCA CACTTTCGAC
    CGCTACCGCA GCCAGTCCAC GGCCAAGGAG AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA
    TCCCAACAGC GGCGACATCA GAGCTGGAGC ACCTTCAAGT CTATGACGCT GGGCTCGCTG
    CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG CACCGGGCAA CAGCCTGGGA ACCCACAGAA
    CCACCGGACG GCGACTTCCA GACGGAGGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227860
    Clone ID Related Accession (Same CDS sequence) XM_024982266.1
    Accession Version XM_024982266.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTGA GCAGGGCCCG
    TGCTGGACCC TGTCCCTGGG CCTCGGGGCG AGGGCAGCTG GAGAGCTTCA GCTTTGGCCC
    CTGGTCTGCT CTCTGGTGCC AGACGTGGCC CCGGCCCTGC TATCTGGGCA CAGGGGTCAG
    CTGGGCTGGG GGTCTGGGAG GATGAAGGAC CCCGAGGATG GGCGGCCATC TCACTCTGGC
    TCCCCCACTC CCCCAGGTCT GCCTGCCCTG GTGGTGGCCG TGTCCGTCGG CTTTACCCGC
    ACCAAAGGAT ACGGTACATC CAGCTACTGC TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT
    GCCTTCGTGG GCCCAGCAGC TGTAATTGTC CTGGTGAATA TGCTCATCGG AATCATCGTC
    TTCAACAAGC TCATGGCTCG CGATGGCATC TCAGACAAGT CCAAGAAGCA GAGGGCCGGG
    GCCTCACTCT GGAGCTCCTG TGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCTGCC
    GTCCTGGCCA TGACAGACCG CCGCTCCGTC CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC
    TCTGCTCAGG GCTTTGTCAT CACGGCCGTC CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTGTGTCGG GCTGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAGAACG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACTGTGC TGTTCAAGGA GGTCAACACT TGCAACCCAT CCACCATCAC GGGCACGCTG
    TCCCGCTTGT CTCTGGACGA GGATGAGGAG CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC
    AGCCTCAGCT TCTCACCACT GCCTGGCAAC ATCCTGGTGC CCATGGCAGC CTCACCAGGG
    CTGGGGGAGC CACCGCCCCC GCAGGAGGCC AACCCCGTGT ACATGTGTGG AGAGGGCGGC
    CTGCGGCAGC TGGACCTCAC ATGGCTGCGG CCGGCCGAGC CGGGCTCCGA GGGGGACTAC
    ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG
    GATCCCCCGC GGGCCCGGCC TGAGGGCACC CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC
    ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG GAACACTCGG GCCTGGGGCT GGGCCCTGCC
    TATGGGTCTC TACAGAACCC CTACGGGATG ACCTTCCAAC CGCCGCCGCC AACACCCAGC
    GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC AGCCGGACCA TGCCCCGAAC TGTGCCTGGC
    TCCACCATGA AGCTGGGCTC CCTGGAGCGA AAGAAGTTAC GGTATTCAGA CCTGGACTTT
    GAGAAGGTGA TGCACACCCG GAAACGGCAC TCGGAACTCT ACCACGAGCT CAACCAGAAG
    TTCCACACTT TCGACCGCTA CCGCAGCCAG TCCACGGCCA AGGAGAAGCC CAGTCCAGGG
    GAGCGTCCCG GCTTATCCCA ACAGCGGCGA CATCAGAGCT GGAGCACCTT CAAGTCTATG
    ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA GAGCGGCTGG CCCTGCACCG GGCAACAGCC
    TGGGAACCCA CAGAACCACC GGACGGCGAC TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838034.1
    CDS167..4954
    Translation

    Target ORF information:

    RefSeq Version XM_024982266.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTGA GCAGGGCCCG
    TGCTGGACCC TGTCCCTGGG CCTCGGGGCG AGGGCAGCTG GAGAGCTTCA GCTTTGGCCC
    CTGGTCTGCT CTCTGGTGCC AGACGTGGCC CCGGCCCTGC TATCTGGGCA CAGGGGTCAG
    CTGGGCTGGG GGTCTGGGAG GATGAAGGAC CCCGAGGATG GGCGGCCATC TCACTCTGGC
    TCCCCCACTC CCCCAGGTCT GCCTGCCCTG GTGGTGGCCG TGTCCGTCGG CTTTACCCGC
    ACCAAAGGAT ACGGTACATC CAGCTACTGC TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT
    GCCTTCGTGG GCCCAGCAGC TGTAATTGTC CTGGTGAATA TGCTCATCGG AATCATCGTC
    TTCAACAAGC TCATGGCTCG CGATGGCATC TCAGACAAGT CCAAGAAGCA GAGGGCCGGG
    GCCTCACTCT GGAGCTCCTG TGTGGTGCTG CCCCTGCTGG CGCTCACCTG GATGTCTGCC
    GTCCTGGCCA TGACAGACCG CCGCTCCGTC CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC
    TCTGCTCAGG GCTTTGTCAT CACGGCCGTC CACTGCTTCC TGCGCCGAGA GGTCCAGGAC
    GTGGTGAAGT GCCAGATGGG TGTGTGTCGG GCTGATGAGA GTGAAGACTC CCCCGACTCA
    TGTAAGAACG GGCAGCTGCA GATCCTGTCA GACTTTGAAA AAGATGTGGA TCTGGCTTGT
    CAGACTGTGC TGTTCAAGGA GGTCAACACT TGCAACCCAT CCACCATCAC GGGCACGCTG
    TCCCGCTTGT CTCTGGACGA GGATGAGGAG CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC
    AGCCTCAGCT TCTCACCACT GCCTGGCAAC ATCCTGGTGC CCATGGCAGC CTCACCAGGG
    CTGGGGGAGC CACCGCCCCC GCAGGAGGCC AACCCCGTGT ACATGTGTGG AGAGGGCGGC
    CTGCGGCAGC TGGACCTCAC ATGGCTGCGG CCGGCCGAGC CGGGCTCCGA GGGGGACTAC
    ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG
    GATCCCCCGC GGGCCCGGCC TGAGGGCACC CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC
    ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG GAACACTCGG GCCTGGGGCT GGGCCCTGCC
    TATGGGTCTC TACAGAACCC CTACGGGATG ACCTTCCAAC CGCCGCCGCC AACACCCAGC
    GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC AGCCGGACCA TGCCCCGAAC TGTGCCTGGC
    TCCACCATGA AGCTGGGCTC CCTGGAGCGA AAGAAGTTAC GGTATTCAGA CCTGGACTTT
    GAGAAGGTGA TGCACACCCG GAAACGGCAC TCGGAACTCT ACCACGAGCT CAACCAGAAG
    TTCCACACTT TCGACCGCTA CCGCAGCCAG TCCACGGCCA AGGAGAAGCC CAGTCCAGGG
    GAGCGTCCCG GCTTATCCCA ACAGCGGCGA CATCAGAGCT GGAGCACCTT CAAGTCTATG
    ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA GAGCGGCTGG CCCTGCACCG GGCAACAGCC
    TGGGAACCCA CAGAACCACC GGACGGCGAC TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227861
    Clone ID Related Accession (Same CDS sequence) XM_024982268.1
    Accession Version XM_024982268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG TCGGAGCGGT GCCCCTGGGC CAGCCTGCTC
    CTCCCCTGCT CAGCGTGTGG AGCGGTCCCC AGCCCCCTGC TCAGCTCAGC CTCGGCCAGG
    AACGCCATGG CCTCACTCTG GAGCTCCTGT GTGGTGCTGC CCCTGCTGGC GCTCACCTGG
    ATGTCTGCCG TCCTGGCCAT GACAGACCGC CGCTCCGTCC TCTTCCAGGC CCTGTTCGCG
    GTCTTCAACT CTGCTCAGGG CTTTGTCATC ACGGCCGTCC ACTGCTTCCT GCGCCGAGAG
    GTCCAGGACG TGGTGAAGTG CCAGATGGGT GTGTGTCGGG CTGATGAGAG TGAAGACTCC
    CCCGACTCAT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA AGATGTGGAT
    CTGGCTTGTC AGACTGTGCT GTTCAAGGAG GTCAACACTT GCAACCCATC CACCATCACG
    GGCACGCTGT CCCGCTTGTC TCTGGACGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC
    CCCGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGCAACA TCCTGGTGCC CATGGCAGCC
    TCACCAGGGC TGGGGGAGCC ACCGCCCCCG CAGGAGGCCA ACCCCGTGTA CATGTGTGGA
    GAGGGCGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CGGCCGAGCC GGGCTCCGAG
    GGGGACTACA TGGTGCTGCC CCGGAGGACT CTGAGTCTGC AGCCTGGCGG CGGGGGTGGA
    GGTGGCGAGG ATCCCCCGCG GGCCCGGCCT GAGGGCACCC CCCGGCGGGC CTCCAAGTCC
    CTGGGCCACA CCGAAGCCTA CCCCAGCTTC CTGTCTGTGG AACACTCGGG CCTGGGGCTG
    GGCCCTGCCT ATGGGTCTCT ACAGAACCCC TACGGGATGA CCTTCCAACC GCCGCCGCCA
    ACACCCAGCG CCCGCCAGGT GCCAGAGCCG GGGGAGCGCA GCCGGACCAT GCCCCGAACT
    GTGCCTGGCT CCACCATGAA GCTGGGCTCC CTGGAGCGAA AGAAGTTACG GTATTCAGAC
    CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCACT CGGAACTCTA CCACGAGCTC
    AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GGAGAAGCCC
    AGTCCAGGGG AGCGTCCCGG CTTATCCCAA CAGCGGCGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CGCTGGGCTC GCTGCCCCCC AAGCCCCGAG AGCGGCTGGC CCTGCACCGG
    GCAACAGCCT GGGAACCCAC AGAACCACCG GACGGCGACT TCCAGACGGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838036.1
    CDS166..4842
    Translation

    Target ORF information:

    RefSeq Version XM_024982268.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG TCGGAGCGGT GCCCCTGGGC CAGCCTGCTC
    CTCCCCTGCT CAGCGTGTGG AGCGGTCCCC AGCCCCCTGC TCAGCTCAGC CTCGGCCAGG
    AACGCCATGG CCTCACTCTG GAGCTCCTGT GTGGTGCTGC CCCTGCTGGC GCTCACCTGG
    ATGTCTGCCG TCCTGGCCAT GACAGACCGC CGCTCCGTCC TCTTCCAGGC CCTGTTCGCG
    GTCTTCAACT CTGCTCAGGG CTTTGTCATC ACGGCCGTCC ACTGCTTCCT GCGCCGAGAG
    GTCCAGGACG TGGTGAAGTG CCAGATGGGT GTGTGTCGGG CTGATGAGAG TGAAGACTCC
    CCCGACTCAT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA AGATGTGGAT
    CTGGCTTGTC AGACTGTGCT GTTCAAGGAG GTCAACACTT GCAACCCATC CACCATCACG
    GGCACGCTGT CCCGCTTGTC TCTGGACGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC
    CCCGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGCAACA TCCTGGTGCC CATGGCAGCC
    TCACCAGGGC TGGGGGAGCC ACCGCCCCCG CAGGAGGCCA ACCCCGTGTA CATGTGTGGA
    GAGGGCGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CGGCCGAGCC GGGCTCCGAG
    GGGGACTACA TGGTGCTGCC CCGGAGGACT CTGAGTCTGC AGCCTGGCGG CGGGGGTGGA
    GGTGGCGAGG ATCCCCCGCG GGCCCGGCCT GAGGGCACCC CCCGGCGGGC CTCCAAGTCC
    CTGGGCCACA CCGAAGCCTA CCCCAGCTTC CTGTCTGTGG AACACTCGGG CCTGGGGCTG
    GGCCCTGCCT ATGGGTCTCT ACAGAACCCC TACGGGATGA CCTTCCAACC GCCGCCGCCA
    ACACCCAGCG CCCGCCAGGT GCCAGAGCCG GGGGAGCGCA GCCGGACCAT GCCCCGAACT
    GTGCCTGGCT CCACCATGAA GCTGGGCTCC CTGGAGCGAA AGAAGTTACG GTATTCAGAC
    CTGGACTTTG AGAAGGTGAT GCACACCCGG AAACGGCACT CGGAACTCTA CCACGAGCTC
    AACCAGAAGT TCCACACTTT CGACCGCTAC CGCAGCCAGT CCACGGCCAA GGAGAAGCCC
    AGTCCAGGGG AGCGTCCCGG CTTATCCCAA CAGCGGCGAC ATCAGAGCTG GAGCACCTTC
    AAGTCTATGA CGCTGGGCTC GCTGCCCCCC AAGCCCCGAG AGCGGCTGGC CCTGCACCGG
    GCAACAGCCT GGGAACCCAC AGAACCACCG GACGGCGACT TCCAGACGGA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227862
    Clone ID Related Accession (Same CDS sequence) XM_010802505.3
    Accession Version XM_010802505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG TCGGAGCGGT GCCCCTGGGC CAGCCTGCTC
    CTCCCCTGCT CAGCGTGTGG AGCGGTCCCC AGCCCCCTGC TCAGCTCAGC CTCGGCCAGG
    AACGCCATGG CCTCACTCTG GAGCTCCTGT GTGGTGCTGC CCCTGCTGGC GCTCACCTGG
    ATGTCTGCCG TCCTGGCCAT GACAGACCGC CGCTCCGTCC TCTTCCAGGC CCTGTTCGCG
    GTCTTCAACT CTGCTCAGGG CTTTGTCATC ACGGCCGTCC ACTGCTTCCT GCGCCGAGAG
    GTCCAGGACG TGGTGAAGTG CCAGATGGGT GTGTGTCGGG CTGATGAGAG TGAAGACTCC
    CCCGACTCAT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA AGATGTGGAT
    CTGGCTTGTC AGACTGTGCT GTTCAAGGAG GTCAACACTT GCAACCCATC CACCATCACG
    GGCACGCTGT CCCGCTTGTC TCTGGACGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC
    CCCGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGCAACA TCCTGGTGCC CATGGCAGCC
    TCACCAGGGC TGGGGGAGCC ACCGCCCCCG CAGGAGGCCA ACCCCGTGTA CATGTGTGGA
    GAGGGCGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CGGCCGAGCC GGGCTCCGAG
    GGGGACTACA TGGTGCTGCC CCGGAGGACT CTGAGTCTGC AGCCTGGCGG CGGGGGTGGA
    GGTGGCGAGG ATCCCCCGCG GGCCCGGCCT GAGGGCACCC CCCGGCGGGC CTCCAAGTCC
    CTGGGCCACA CCGAAGCCTA CCCCAGCTTC CTGTCTGTGG AACACTCGGG CCTGGGGCTG
    GGCCCTGCCT ATGGGTCTCT ACAGAACCCC TACGGGATGA CCTTCCAACC GCCGCCGCCA
    ACACCCAGCG CCCGCCAGGT GCCAGAGCCG GGGGAGCGCA GCCGGACCAT GCCCCGAACT
    GTGCCTGGCT CCACCATGAA GCTGGGCTCC CTGGAGCGAA AGAAGTTACG GTATTCAGAC
    CTGGACTTTG AGGTGATGCA CACCCGGAAA CGGCACTCGG AACTCTACCA CGAGCTCAAC
    CAGAAGTTCC ACACTTTCGA CCGCTACCGC AGCCAGTCCA CGGCCAAGGA GAAGCCCAGT
    CCAGGGGAGC GTCCCGGCTT ATCCCAACAG CGGCGACATC AGAGCTGGAG CACCTTCAAG
    TCTATGACGC TGGGCTCGCT GCCCCCCAAG CCCCGAGAGC GGCTGGCCCT GCACCGGGCA
    ACAGCCTGGG AACCCACAGA ACCACCGGAC GGCGACTTCC AGACGGAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800807.2
    CDS167..4840
    Translation

    Target ORF information:

    RefSeq Version XM_010802505.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG TCGGAGCGGT GCCCCTGGGC CAGCCTGCTC
    CTCCCCTGCT CAGCGTGTGG AGCGGTCCCC AGCCCCCTGC TCAGCTCAGC CTCGGCCAGG
    AACGCCATGG CCTCACTCTG GAGCTCCTGT GTGGTGCTGC CCCTGCTGGC GCTCACCTGG
    ATGTCTGCCG TCCTGGCCAT GACAGACCGC CGCTCCGTCC TCTTCCAGGC CCTGTTCGCG
    GTCTTCAACT CTGCTCAGGG CTTTGTCATC ACGGCCGTCC ACTGCTTCCT GCGCCGAGAG
    GTCCAGGACG TGGTGAAGTG CCAGATGGGT GTGTGTCGGG CTGATGAGAG TGAAGACTCC
    CCCGACTCAT GTAAGAACGG GCAGCTGCAG ATCCTGTCAG ACTTTGAAAA AGATGTGGAT
    CTGGCTTGTC AGACTGTGCT GTTCAAGGAG GTCAACACTT GCAACCCATC CACCATCACG
    GGCACGCTGT CCCGCTTGTC TCTGGACGAG GATGAGGAGC CCAAGTCCTG CCTCGTGGGC
    CCCGAGGGCA GCCTCAGCTT CTCACCACTG CCTGGCAACA TCCTGGTGCC CATGGCAGCC
    TCACCAGGGC TGGGGGAGCC ACCGCCCCCG CAGGAGGCCA ACCCCGTGTA CATGTGTGGA
    GAGGGCGGCC TGCGGCAGCT GGACCTCACA TGGCTGCGGC CGGCCGAGCC GGGCTCCGAG
    GGGGACTACA TGGTGCTGCC CCGGAGGACT CTGAGTCTGC AGCCTGGCGG CGGGGGTGGA
    GGTGGCGAGG ATCCCCCGCG GGCCCGGCCT GAGGGCACCC CCCGGCGGGC CTCCAAGTCC
    CTGGGCCACA CCGAAGCCTA CCCCAGCTTC CTGTCTGTGG AACACTCGGG CCTGGGGCTG
    GGCCCTGCCT ATGGGTCTCT ACAGAACCCC TACGGGATGA CCTTCCAACC GCCGCCGCCA
    ACACCCAGCG CCCGCCAGGT GCCAGAGCCG GGGGAGCGCA GCCGGACCAT GCCCCGAACT
    GTGCCTGGCT CCACCATGAA GCTGGGCTCC CTGGAGCGAA AGAAGTTACG GTATTCAGAC
    CTGGACTTTG AGGTGATGCA CACCCGGAAA CGGCACTCGG AACTCTACCA CGAGCTCAAC
    CAGAAGTTCC ACACTTTCGA CCGCTACCGC AGCCAGTCCA CGGCCAAGGA GAAGCCCAGT
    CCAGGGGAGC GTCCCGGCTT ATCCCAACAG CGGCGACATC AGAGCTGGAG CACCTTCAAG
    TCTATGACGC TGGGCTCGCT GCCCCCCAAG CCCCGAGAGC GGCTGGCCCT GCACCGGGCA
    ACAGCCTGGG AACCCACAGA ACCACCGGAC GGCGACTTCC AGACGGAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227863
    Clone ID Related Accession (Same CDS sequence) XM_024982274.1
    Accession Version XM_024982274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTGAGCAGG GCCCGTGCTG GACCCTGTCC CTGGGCCTCG GGGCGAGGGC AGCTGGAGAG
    CTTCAGCTTT GGCCCCTGGT CTGCTCTCTG GTGCCAGACG TGGCCCCGGC CCTGCTATCT
    GGGCACAGGG GTCAGCTGGG CTGGGGGTCT GGGAGGATGA AGGACCCCGA GGATGGGCGG
    CCATCTCACT CTGGCTCCCC CACTCCCCCA GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC
    GTCGGCTTTA CCCGCACCAA AGGATACGGT ACATCCAGCT ACTGCTGGCT CTCTCTGGAG
    GGTGGCCTGC TTTATGCCTT CGTGGGCCCA GCAGCTGTAA TTGTCCTGGT GAATATGCTC
    ATCGGAATCA TCGTCTTCAA CAAGCTCATG GCTCGCGATG GCATCTCAGA CAAGTCCAAG
    AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CTGCCGTCCT GGCCATGACA GACCGCCGCT CCGTCCTCTT CCAGGCCCTG
    TTCGCGGTCT TCAACTCTGC TCAGGGCTTT GTCATCACGG CCGTCCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC TGTGCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCCACC
    ATCACGGGCA CGCTGTCCCG CTTGTCTCTG GACGAGGATG AGGAGCCCAA GTCCTGCCTC
    GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA CCACTGCCTG GCAACATCCT GGTGCCCATG
    GCAGCCTCAC CAGGGCTGGG GGAGCCACCG CCCCCGCAGG AGGCCAACCC CGTGTACATG
    TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC CTCACATGGC TGCGGCCGGC CGAGCCGGGC
    TCCGAGGGGG ACTACATGGT GCTGCCCCGG AGGACTCTGA GTCTGCAGCC TGGCGGCGGG
    GGTGGAGGTG GCGAGGATCC CCCGCGGGCC CGGCCTGAGG GCACCCCCCG GCGGGCCTCC
    AAGTCCCTGG GCCACACCGA AGCCTACCCC AGCTTCCTGT CTGTGGAACA CTCGGGCCTG
    GGGCTGGGCC CTGCCTATGG GTCTCTACAG AACCCCTACG GGATGACCTT CCAACCGCCG
    CCGCCAACAC CCAGCGCCCG CCAGGTGCCA GAGCCGGGGG AGCGCAGCCG GACCATGCCC
    CGAACTGTGC CTGGCTCCAC CATGAAGCTG GGCTCCCTGG AGCGAAAGAA GTTACGGTAT
    TCAGACCTGG ACTTTGAGAA GGTGATGCAC ACCCGGAAAC GGCACTCGGA ACTCTACCAC
    GAGCTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGCA GCCAGTCCAC GGCCAAGGAG
    AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA TCCCAACAGC GGCGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACGCT GGGCTCGCTG CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG
    CACCGGGCAA CAGCCTGGGA ACCCACAGAA CCACCGGACG GCGACTTCCA GACGGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838042.1
    CDS167..4789
    Translation

    Target ORF information:

    RefSeq Version XM_024982274.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTGAGCAGG GCCCGTGCTG GACCCTGTCC CTGGGCCTCG GGGCGAGGGC AGCTGGAGAG
    CTTCAGCTTT GGCCCCTGGT CTGCTCTCTG GTGCCAGACG TGGCCCCGGC CCTGCTATCT
    GGGCACAGGG GTCAGCTGGG CTGGGGGTCT GGGAGGATGA AGGACCCCGA GGATGGGCGG
    CCATCTCACT CTGGCTCCCC CACTCCCCCA GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC
    GTCGGCTTTA CCCGCACCAA AGGATACGGT ACATCCAGCT ACTGCTGGCT CTCTCTGGAG
    GGTGGCCTGC TTTATGCCTT CGTGGGCCCA GCAGCTGTAA TTGTCCTGGT GAATATGCTC
    ATCGGAATCA TCGTCTTCAA CAAGCTCATG GCTCGCGATG GCATCTCAGA CAAGTCCAAG
    AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CTGCCGTCCT GGCCATGACA GACCGCCGCT CCGTCCTCTT CCAGGCCCTG
    TTCGCGGTCT TCAACTCTGC TCAGGGCTTT GTCATCACGG CCGTCCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC TGTGCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCCACC
    ATCACGGGCA CGCTGTCCCG CTTGTCTCTG GACGAGGATG AGGAGCCCAA GTCCTGCCTC
    GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA CCACTGCCTG GCAACATCCT GGTGCCCATG
    GCAGCCTCAC CAGGGCTGGG GGAGCCACCG CCCCCGCAGG AGGCCAACCC CGTGTACATG
    TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC CTCACATGGC TGCGGCCGGC CGAGCCGGGC
    TCCGAGGGGG ACTACATGGT GCTGCCCCGG AGGACTCTGA GTCTGCAGCC TGGCGGCGGG
    GGTGGAGGTG GCGAGGATCC CCCGCGGGCC CGGCCTGAGG GCACCCCCCG GCGGGCCTCC
    AAGTCCCTGG GCCACACCGA AGCCTACCCC AGCTTCCTGT CTGTGGAACA CTCGGGCCTG
    GGGCTGGGCC CTGCCTATGG GTCTCTACAG AACCCCTACG GGATGACCTT CCAACCGCCG
    CCGCCAACAC CCAGCGCCCG CCAGGTGCCA GAGCCGGGGG AGCGCAGCCG GACCATGCCC
    CGAACTGTGC CTGGCTCCAC CATGAAGCTG GGCTCCCTGG AGCGAAAGAA GTTACGGTAT
    TCAGACCTGG ACTTTGAGAA GGTGATGCAC ACCCGGAAAC GGCACTCGGA ACTCTACCAC
    GAGCTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGCA GCCAGTCCAC GGCCAAGGAG
    AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA TCCCAACAGC GGCGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACGCT GGGCTCGCTG CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG
    CACCGGGCAA CAGCCTGGGA ACCCACAGAA CCACCGGACG GCGACTTCCA GACGGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227864
    Clone ID Related Accession (Same CDS sequence) XM_024982275.1
    Accession Version XM_024982275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTGAGCAGG GCCCGTGCTG GACCCTGTCC CTGGGCCTCG GGGCGAGGGC AGCTGGAGAG
    CTTCAGCTTT GGCCCCTGGT CTGCTCTCTG GTGCCAGACG TGGCCCCGGC CCTGCTATCT
    GGGCACAGGG GTCAGCTGGG CTGGGGGTCT GGGAGGATGA AGGACCCCGA GGATGGGCGG
    CCATCTCACT CTGGCTCCCC CACTCCCCCA GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC
    GTCGGCTTTA CCCGCACCAA AGGATACGGT ACATCCAGCT ACTGCTGGCT CTCTCTGGAG
    GGTGGCCTGC TTTATGCCTT CGTGGGCCCA GCAGCTGTAA TTGTCCTGGT GAATATGCTC
    ATCGGAATCA TCGTCTTCAA CAAGCTCATG GCTCGCGATG GCATCTCAGA CAAGTCCAAG
    AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CTGCCGTCCT GGCCATGACA GACCGCCGCT CCGTCCTCTT CCAGGCCCTG
    TTCGCGGTCT TCAACTCTGC TCAGGGCTTT GTCATCACGG CCGTCCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC TGTGCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCCACC
    ATCACGGGCA CGCTGTCCCG CTTGTCTCTG GACGAGGATG AGGAGCCCAA GTCCTGCCTC
    GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA CCACTGCCTG GCAACATCCT GGTGCCCATG
    GCAGCCTCAC CAGGGCTGGG GGAGCCACCG CCCCCGCAGG AGGCCAACCC CGTGTACATG
    TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC CTCACATGGC TGCGGCCGGC CGAGCCGGGC
    TCCGAGGGGG ACTACATGGT GCTGCCCCGG AGGACTCTGA GTCTGCAGCC TGGCGGCGGG
    GGTGGAGGTG GCGAGGATCC CCCGCGGGCC CGGCCTGAGG GCACCCCCCG GCGGGCCTCC
    AAGTCCCTGG GCCACACCGA AGCCTACCCC AGCTTCCTGT CTGTGGAACA CTCGGGCCTG
    GGGCTGGGCC CTGCCTATGG GTCTCTACAG AACCCCTACG GGATGACCTT CCAACCGCCG
    CCGCCAACAC CCAGCGCCCG CCAGGTGCCA GAGCCGGGGG AGCGCAGCCG GACCATGCCC
    CGAACTGTGC CTGGCTCCAC CATGAAGCTG GGCTCCCTGG AGCGAAAGAA GTTACGGTAT
    TCAGACCTGG ACTTTGAGAA GGTGATGCAC ACCCGGAAAC GGCACTCGGA ACTCTACCAC
    GAGCTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGCA GCCAGTCCAC GGCCAAGGAG
    AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA TCCCAACAGC GGCGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACGCT GGGCTCGCTG CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG
    CACCGGGCAA CAGCCTGGGA ACCCACAGAA CCACCGGACG GCGACTTCCA GACGGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838043.1
    CDS167..4789
    Translation

    Target ORF information:

    RefSeq Version XM_024982275.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTGAGCAGG GCCCGTGCTG GACCCTGTCC CTGGGCCTCG GGGCGAGGGC AGCTGGAGAG
    CTTCAGCTTT GGCCCCTGGT CTGCTCTCTG GTGCCAGACG TGGCCCCGGC CCTGCTATCT
    GGGCACAGGG GTCAGCTGGG CTGGGGGTCT GGGAGGATGA AGGACCCCGA GGATGGGCGG
    CCATCTCACT CTGGCTCCCC CACTCCCCCA GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC
    GTCGGCTTTA CCCGCACCAA AGGATACGGT ACATCCAGCT ACTGCTGGCT CTCTCTGGAG
    GGTGGCCTGC TTTATGCCTT CGTGGGCCCA GCAGCTGTAA TTGTCCTGGT GAATATGCTC
    ATCGGAATCA TCGTCTTCAA CAAGCTCATG GCTCGCGATG GCATCTCAGA CAAGTCCAAG
    AAGCAGAGGG CCGGGGCCTC ACTCTGGAGC TCCTGTGTGG TGCTGCCCCT GCTGGCGCTC
    ACCTGGATGT CTGCCGTCCT GGCCATGACA GACCGCCGCT CCGTCCTCTT CCAGGCCCTG
    TTCGCGGTCT TCAACTCTGC TCAGGGCTTT GTCATCACGG CCGTCCACTG CTTCCTGCGC
    CGAGAGGTCC AGGACGTGGT GAAGTGCCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA
    GACTCCCCCG ACTCATGTAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAAGAT
    GTGGATCTGG CTTGTCAGAC TGTGCTGTTC AAGGAGGTCA ACACTTGCAA CCCATCCACC
    ATCACGGGCA CGCTGTCCCG CTTGTCTCTG GACGAGGATG AGGAGCCCAA GTCCTGCCTC
    GTGGGCCCCG AGGGCAGCCT CAGCTTCTCA CCACTGCCTG GCAACATCCT GGTGCCCATG
    GCAGCCTCAC CAGGGCTGGG GGAGCCACCG CCCCCGCAGG AGGCCAACCC CGTGTACATG
    TGTGGAGAGG GCGGCCTGCG GCAGCTGGAC CTCACATGGC TGCGGCCGGC CGAGCCGGGC
    TCCGAGGGGG ACTACATGGT GCTGCCCCGG AGGACTCTGA GTCTGCAGCC TGGCGGCGGG
    GGTGGAGGTG GCGAGGATCC CCCGCGGGCC CGGCCTGAGG GCACCCCCCG GCGGGCCTCC
    AAGTCCCTGG GCCACACCGA AGCCTACCCC AGCTTCCTGT CTGTGGAACA CTCGGGCCTG
    GGGCTGGGCC CTGCCTATGG GTCTCTACAG AACCCCTACG GGATGACCTT CCAACCGCCG
    CCGCCAACAC CCAGCGCCCG CCAGGTGCCA GAGCCGGGGG AGCGCAGCCG GACCATGCCC
    CGAACTGTGC CTGGCTCCAC CATGAAGCTG GGCTCCCTGG AGCGAAAGAA GTTACGGTAT
    TCAGACCTGG ACTTTGAGAA GGTGATGCAC ACCCGGAAAC GGCACTCGGA ACTCTACCAC
    GAGCTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGCA GCCAGTCCAC GGCCAAGGAG
    AAGCCCAGTC CAGGGGAGCG TCCCGGCTTA TCCCAACAGC GGCGACATCA GAGCTGGAGC
    ACCTTCAAGT CTATGACGCT GGGCTCGCTG CCCCCCAAGC CCCGAGAGCG GCTGGCCCTG
    CACCGGGCAA CAGCCTGGGA ACCCACAGAA CCACCGGACG GCGACTTCCA GACGGAGGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227865
    Clone ID Related Accession (Same CDS sequence) XM_024982281.1
    Accession Version XM_024982281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGAAGGTGA TGCACACCCG GAAACGGCAC
    TCGGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG
    TCCACGGCCA AGGAGAAGCC CAGTCCAGGG GAGCGTCCCG GCTTATCCCA ACAGCGGCGA
    CATCAGAGCT GGAGCACCTT CAAGTCTATG ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA
    GAGCGGCTGG CCCTGCACCG GGCAACAGCC TGGGAACCCA CAGAACCACC GGACGGCGAC
    TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838049.1
    CDS166..4743
    Translation

    Target ORF information:

    RefSeq Version XM_024982281.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGAAGGTGA TGCACACCCG GAAACGGCAC
    TCGGAACTCT ACCACGAGCT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGCAGCCAG
    TCCACGGCCA AGGAGAAGCC CAGTCCAGGG GAGCGTCCCG GCTTATCCCA ACAGCGGCGA
    CATCAGAGCT GGAGCACCTT CAAGTCTATG ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA
    GAGCGGCTGG CCCTGCACCG GGCAACAGCC TGGGAACCCA CAGAACCACC GGACGGCGAC
    TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227866
    Clone ID Related Accession (Same CDS sequence) XM_024982291.1
    Accession Version XM_024982291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC GTCGGCTTTA CCCGCACCAA AGGATACGGT
    ACATCCAGCT ACTGCTGGCT CTCTCTGGAG GGTGGCCTGC TTTATGCCTT CGTGGGCCCA
    GCAGCTGTAA TTGTCCTGGT GAATATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG
    GCTCGCGATG GCATCTCAGA CAAGTCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC
    TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC
    TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGTGTGGT GCTGCCCCTG
    CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCCATGACAG ACCGCCGCTC CGTCCTCTTC
    CAGGCCCTGT TCGCGGTCTT CAACTCTGCT CAGGGCTTTG TCATCACGGC CGTCCACTGC
    TTCCTGCGCC GAGAGGTCCA GGACGTGGTG AAGTGCCAGA TGGGTGTGTG TCGGGCTGAT
    GAGAGTGAAG ACTCCCCCGA CTCATGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT
    GAAAAAGATG TGGATCTGGC TTGTCAGACT GTGCTGTTCA AGGAGGTCAA CACTTGCAAC
    CCATCCACCA TCACGGGCAC GCTGTCCCGC TTGTCTCTGG ACGAGGATGA GGAGCCCAAG
    TCCTGCCTCG TGGGCCCCGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG CAACATCCTG
    GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCACCGC CCCCGCAGGA GGCCAACCCC
    GTGTACATGT GTGGAGAGGG CGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCGGCC
    GAGCCGGGCT CCGAGGGGGA CTACATGGTG CTGCCCCGGA GGACTCTGAG TCTGCAGCCT
    GGCGGCGGGG GTGGAGGTGG CGAGGATCCC CCGCGGGCCC GGCCTGAGGG CACCCCCCGG
    CGGGCCTCCA AGTCCCTGGG CCACACCGAA GCCTACCCCA GCTTCCTGTC TGTGGAACAC
    TCGGGCCTGG GGCTGGGCCC TGCCTATGGG TCTCTACAGA ACCCCTACGG GATGACCTTC
    CAACCGCCGC CGCCAACACC CAGCGCCCGC CAGGTGCCAG AGCCGGGGGA GCGCAGCCGG
    ACCATGCCCC GAACTGTGCC TGGCTCCACC ATGAAGCTGG GCTCCCTGGA GCGAAAGAAG
    TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCACTCGGAA
    CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG
    GCCAAGGAGA AGCCCAGTCC AGGGGAGCGT CCCGGCTTAT CCCAACAGCG GCGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACGCTG GGCTCGCTGC CCCCCAAGCC CCGAGAGCGG
    CTGGCCCTGC ACCGGGCAAC AGCCTGGGAA CCCACAGAAC CACCGGACGG CGACTTCCAG
    ACGGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838059.1
    CDS166..4677
    Translation

    Target ORF information:

    RefSeq Version XM_024982291.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGTGCAC
    GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCC
    CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC
    GAGCTGCAGA CTAAGCTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA
    TGGGGTCCCT GGGGCCCATG TTCCACGTCT TGTGCCAACG GGACCCAGCA GCGCAGCCGG
    AAGTGCAGCG TGGCAGGCCC AGCCTGGGCC ACGTGTGCCG GGGCCCTCAC TGACACCCGG
    GAGTGCGGCA ACCTTGAGTG CCCGGCCACA GATGGCAAGT GGGGGCCATG GAATGCGTGG
    AGCCTGTGTT CCAAGACATG TGACACAGGC TGGCAGCGCC GTTTCCGCAT GTGCCAGGCC
    ACAGGCGCTC AGGGCTACCC CTGCGAGGGC ACTGGCGAGG AGGTGAAGCC CTGCAGCGAG
    AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGACGAGT ACGTGATGCT GATGACATGG
    AAGAAGGCGG CTGCTGGCGA GATCATCTAC AACAAGTGCC CCCCCAATGC CTCAGGGTCT
    GCCAGCCGCC GCTGTCTGCT CAGTGCCCAG GGTGTGGCCT ACTGGGGGCT GCCCAGCTTT
    GCCCGCTGCA TCTCCCACGA GTACCGCTAC CTGTATCTGT CACTTCGGGA GCACCTGGCC
    AAGGGGCAGC GCATGCTGGC GGGCGAGGGC ATGTCTCAGG TGGTGCGCAG CCTGCAGGAG
    CTCCTGGCCC GGCGCACTTA CTACAGCGGG GACCTGCTTT TCTCCGTGGA CATTCTGAGG
    AACGTCACCG ACACCTTCAA GAGAGCCACC TACGTGCCCT CGGCTGATGA CGTGCAGCGC
    TTCTTCCAGG TGGTGAGCTT CATGGTAGAT GCAGAGAACA AGGACAAGTG GGATGACGCT
    CAGCAGGTGT CCCCAGGCTC TGTGCACCTG CTCCGGGTGG TGGAAGACTT CATTCACCTG
    GTGGGCGATG CTCTCAAGGC CTTCCAGAGC TCTCTGATTG TCACAGATAA CCTGGTGATC
    AGCATTCAGC GAGAGCCTGT CTCCGCTGTT TCCAGCGACA TCACGTTCCC CATGCGGGGC
    CGCCGGGGCA TGAAGGACTG GGTGCGGTAC TCAGAGGACC GCCTCTTCCT ACCCAAGGAG
    GTGCTCAGCC TCTCCTCCCC AGGGAAGCCG GCCGCATCTG GGGCAGCGGG CAGCCTGGGC
    AGAGGGAGAG GCCCCGGAAC CGTGCCCCCT GGCCCCGGCC ACTCCCACCA GCGCCTCCTG
    CCGGCAGACC CTGAGGATTC CTCCTCCTAC TTTGTGATTG GTGCTGTGCT CTACCGCACC
    CTGGGCCTCA TCCTGCCACC ACCCAGGCCC CCGCTGGCTG TCACATCCAG AGTGATGACA
    GTGACCGTAA GGCCTCCCAC CCAGCCACTA GCTGAGCCCC TCATCACAGT GGAGCTTTCC
    TACATCATCA ATGGCACCAC AGATCCCCAT TGTGCCAGCT GGGACTACTC CAGAGCAGAC
    GCCAGCTCAG GGGACTGGGA TACCGAGAGC TGCCAGACGC TGGAGACACA GGCAGCCCAC
    ACCCGCTGCC AGTGCCAGCG CCTGTCCACC TTCGCCGTGC TGGCCCAGCC GCCCAAGGAC
    CTGACCCTGG AGCTGGCGGG CGCCCCCTCG GTCCCCCTTG TGATCGGCTG TGCTGTGTCC
    TGCATGGCAC TGCTCACCCT GCTTGCCATC TACGCCGCCT TCTGGAGGTT CATAAAATCT
    GAACGCTCCA TCATCTTGCT GAACTTCTGC CTGTCCATCC TGGCATCCAA CATCCTCATC
    CTTGTGGGCC AGTCGCGGGT GCTGAGCAAG GGTGTGTGTA CCATGACCGC CGCCTTCCTG
    CACTTCTTCT TCCTGTCGTC CTTCTGCTGG GTGCTCACTG AGGCCTGGCA GTCCTACCTG
    GCTGTCATCG GGCGGATGCG CACCCGCCTT GTCCGCAAGC GCTTCCTCTG CCTGGGCTGG
    GGTCTGCCTG CCCTGGTGGT GGCCGTGTCC GTCGGCTTTA CCCGCACCAA AGGATACGGT
    ACATCCAGCT ACTGCTGGCT CTCTCTGGAG GGTGGCCTGC TTTATGCCTT CGTGGGCCCA
    GCAGCTGTAA TTGTCCTGGT GAATATGCTC ATCGGAATCA TCGTCTTCAA CAAGCTCATG
    GCTCGCGATG GCATCTCAGA CAAGTCCAAG AAGCAGAGGG CCGGGTCGGA GCGGTGCCCC
    TGGGCCAGCC TGCTCCTCCC CTGCTCAGCG TGTGGAGCGG TCCCCAGCCC CCTGCTCAGC
    TCAGCCTCGG CCAGGAACGC CATGGCCTCA CTCTGGAGCT CCTGTGTGGT GCTGCCCCTG
    CTGGCGCTCA CCTGGATGTC TGCCGTCCTG GCCATGACAG ACCGCCGCTC CGTCCTCTTC
    CAGGCCCTGT TCGCGGTCTT CAACTCTGCT CAGGGCTTTG TCATCACGGC CGTCCACTGC
    TTCCTGCGCC GAGAGGTCCA GGACGTGGTG AAGTGCCAGA TGGGTGTGTG TCGGGCTGAT
    GAGAGTGAAG ACTCCCCCGA CTCATGTAAG AACGGGCAGC TGCAGATCCT GTCAGACTTT
    GAAAAAGATG TGGATCTGGC TTGTCAGACT GTGCTGTTCA AGGAGGTCAA CACTTGCAAC
    CCATCCACCA TCACGGGCAC GCTGTCCCGC TTGTCTCTGG ACGAGGATGA GGAGCCCAAG
    TCCTGCCTCG TGGGCCCCGA GGGCAGCCTC AGCTTCTCAC CACTGCCTGG CAACATCCTG
    GTGCCCATGG CAGCCTCACC AGGGCTGGGG GAGCCACCGC CCCCGCAGGA GGCCAACCCC
    GTGTACATGT GTGGAGAGGG CGGCCTGCGG CAGCTGGACC TCACATGGCT GCGGCCGGCC
    GAGCCGGGCT CCGAGGGGGA CTACATGGTG CTGCCCCGGA GGACTCTGAG TCTGCAGCCT
    GGCGGCGGGG GTGGAGGTGG CGAGGATCCC CCGCGGGCCC GGCCTGAGGG CACCCCCCGG
    CGGGCCTCCA AGTCCCTGGG CCACACCGAA GCCTACCCCA GCTTCCTGTC TGTGGAACAC
    TCGGGCCTGG GGCTGGGCCC TGCCTATGGG TCTCTACAGA ACCCCTACGG GATGACCTTC
    CAACCGCCGC CGCCAACACC CAGCGCCCGC CAGGTGCCAG AGCCGGGGGA GCGCAGCCGG
    ACCATGCCCC GAACTGTGCC TGGCTCCACC ATGAAGCTGG GCTCCCTGGA GCGAAAGAAG
    TTACGGTATT CAGACCTGGA CTTTGAGAAG GTGATGCACA CCCGGAAACG GCACTCGGAA
    CTCTACCACG AGCTCAACCA GAAGTTCCAC ACTTTCGACC GCTACCGCAG CCAGTCCACG
    GCCAAGGAGA AGCCCAGTCC AGGGGAGCGT CCCGGCTTAT CCCAACAGCG GCGACATCAG
    AGCTGGAGCA CCTTCAAGTC TATGACGCTG GGCTCGCTGC CCCCCAAGCC CCGAGAGCGG
    CTGGCCCTGC ACCGGGCAAC AGCCTGGGAA CCCACAGAAC CACCGGACGG CGACTTCCAG
    ACGGAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227869
    Clone ID Related Accession (Same CDS sequence) XM_024982306.1
    Accession Version XM_024982306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G protein-coupled receptor B2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGGTGATGC ACACCCGGAA ACGGCACTCG
    GAACTCTACC ACGAGCTCAA CCAGAAGTTC CACACTTTCG ACCGCTACCG CAGCCAGTCC
    ACGGCCAAGG AGAAGCCCAG TCCAGGGGAG CGTCCCGGCT TATCCCAACA GCGGCGACAT
    CAGAGCTGGA GCACCTTCAA GTCTATGACG CTGGGCTCGC TGCCCCCCAA GCCCCGAGAG
    CGGCTGGCCC TGCACCGGGC AACAGCCTGG GAACCCACAG AACCACCGGA CGGCGACTTC
    CAGACGGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838074.1
    CDS16..4590
    Translation

    Target ORF information:

    RefSeq Version XM_024982306.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACCGCC AGGAGCAGGT GTGTACCCAC
    TTCGCCCCTC GCCTGCTGCC GCTGGACCAC TACCTGGTCA ACTTCACCTG CCTGCGGCCC
    AGCCCCGAGG AGGCAGAGGC CCAGGCGGGG TCGGAGCTGG GGCGGCCCGA GGAGGAGGAG
    AAGGAGGAGG AGGAAGCCGC GGGGTTGGAG CTCTGTGGTG GCTCTGGCCC CTTCACCTTC
    CTGCACTTCG ACAAGAACTT CGTGCAGCTG TGCCTGTCGG CCGAGCCCTC CGAGGCCCCG
    CGCCTGCTGG CGCCCGCGGC CCTGGCCTTC CGCTTCGTCG AGGTCTTGCT CATCAACAAC
    AACAACTCCA GCCAGTTCAC CTGCGGGGTC CTCTGCCGCT GGAGTGAGGA GTGCAGCCGC
    GCGGCCGGCA GGGCCTGTGG CTTCGCCCAG CCGGGCTGCA GCTGCCCCGG TGAGGCGGGG
    GCCGGCCCCG CGGCCACCAC CACGCCTCCT GGTCCGCCTG CTGCCCACAC CCTGTCCAAT
    GCCCTGGTGC CCGGGGGCCC CGCCCCACCT GCCGAGGCTG ATTTGCACTC GGGGAGCAGC
    AATGATCTGT TCACCACGGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG
    AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTGT ACATGGCACA GACAGGCGAC
    CCGGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGCCTG
    CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTG
    CGGGAGACCC GGCCCTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG
    TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCCCGGAG CCGGATGCGG
    ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAG
    CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC
    CCATGTTCCA CGTCTTGTGC CAACGGGACC CAGCAGCGCA GCCGGAAGTG CAGCGTGGCA
    GGCCCAGCCT GGGCCACGTG TGCCGGGGCC CTCACTGACA CCCGGGAGTG CGGCAACCTT
    GAGTGCCCGG CCACAGATGG CAAGTGGGGG CCATGGAATG CGTGGAGCCT GTGTTCCAAG
    ACATGTGACA CAGGCTGGCA GCGCCGTTTC CGCATGTGCC AGGCCACAGG CGCTCAGGGC
    TACCCCTGCG AGGGCACTGG CGAGGAGGTG AAGCCCTGCA GCGAGAAGAG GTGTCCAGCC
    TTCCATGAGA TGTGCAGGGA CGAGTACGTG ATGCTGATGA CATGGAAGAA GGCGGCTGCT
    GGCGAGATCA TCTACAACAA GTGCCCCCCC AATGCCTCAG GGTCTGCCAG CCGCCGCTGT
    CTGCTCAGTG CCCAGGGTGT GGCCTACTGG GGGCTGCCCA GCTTTGCCCG CTGCATCTCC
    CACGAGTACC GCTACCTGTA TCTGTCACTT CGGGAGCACC TGGCCAAGGG GCAGCGCATG
    CTGGCGGGCG AGGGCATGTC TCAGGTGGTG CGCAGCCTGC AGGAGCTCCT GGCCCGGCGC
    ACTTACTACA GCGGGGACCT GCTTTTCTCC GTGGACATTC TGAGGAACGT CACCGACACC
    TTCAAGAGAG CCACCTACGT GCCCTCGGCT GATGACGTGC AGCGCTTCTT CCAGGTGGTG
    AGCTTCATGG TAGATGCAGA GAACAAGGAC AAGTGGGATG ACGCTCAGCA GGTGTCCCCA
    GGCTCTGTGC ACCTGCTCCG GGTGGTGGAA GACTTCATTC ACCTGGTGGG CGATGCTCTC
    AAGGCCTTCC AGAGCTCTCT GATTGTCACA GATAACCTGG TGATCAGCAT TCAGCGAGAG
    CCTGTCTCCG CTGTTTCCAG CGACATCACG TTCCCCATGC GGGGCCGCCG GGGCATGAAG
    GACTGGGTGC GGTACTCAGA GGACCGCCTC TTCCTACCCA AGGAGGTGCT CAGCCTCTCC
    TCCCCAGGGA AGCCGGCCGC ATCTGGGGCA GCGGGCAGCC TGGGCAGAGG GAGAGGCCCC
    GGAACCGTGC CCCCTGGCCC CGGCCACTCC CACCAGCGCC TCCTGCCGGC AGACCCTGAG
    GATTCCTCCT CCTACTTTGT GATTGGTGCT GTGCTCTACC GCACCCTGGG CCTCATCCTG
    CCACCACCCA GGCCCCCGCT GGCTGTCACA TCCAGAGTGA TGACAGTGAC CGTAAGGCCT
    CCCACCCAGC CACTAGCTGA GCCCCTCATC ACAGTGGAGC TTTCCTACAT CATCAATGGC
    ACCACAGATC CCCATTGTGC CAGCTGGGAC TACTCCAGAG CAGACGCCAG CTCAGGGGAC
    TGGGATACCG AGAGCTGCCA GACGCTGGAG ACACAGGCAG CCCACACCCG CTGCCAGTGC
    CAGCGCCTGT CCACCTTCGC CGTGCTGGCC CAGCCGCCCA AGGACCTGAC CCTGGAGCTG
    GCGGGCGCCC CCTCGGTCCC CCTTGTGATC GGCTGTGCTG TGTCCTGCAT GGCACTGCTC
    ACCCTGCTTG CCATCTACGC CGCCTTCTGG AGGTTCATAA AATCTGAACG CTCCATCATC
    TTGCTGAACT TCTGCCTGTC CATCCTGGCA TCCAACATCC TCATCCTTGT GGGCCAGTCG
    CGGGTGCTGA GCAAGGGTGT GTGTACCATG ACCGCCGCCT TCCTGCACTT CTTCTTCCTG
    TCGTCCTTCT GCTGGGTGCT CACTGAGGCC TGGCAGTCCT ACCTGGCTGT CATCGGGCGG
    ATGCGCACCC GCCTTGTCCG CAAGCGCTTC CTCTGCCTGG GCTGGGGTCT GCCTGCCCTG
    GTGGTGGCCG TGTCCGTCGG CTTTACCCGC ACCAAAGGAT ACGGTACATC CAGCTACTGC
    TGGCTCTCTC TGGAGGGTGG CCTGCTTTAT GCCTTCGTGG GCCCAGCAGC TGTAATTGTC
    CTGGTGAATA TGCTCATCGG AATCATCGTC TTCAACAAGC TCATGGCTCG CGATGGCATC
    TCAGACAAGT CCAAGAAGCA GAGGGCCGGG GCCTCACTCT GGAGCTCCTG TGTGGTGCTG
    CCCCTGCTGG CGCTCACCTG GATGTCTGCC GTCCTGGCCA TGACAGACCG CCGCTCCGTC
    CTCTTCCAGG CCCTGTTCGC GGTCTTCAAC TCTGCTCAGG GCTTTGTCAT CACGGCCGTC
    CACTGCTTCC TGCGCCGAGA GGTCCAGGAC GTGGTGAAGT GCCAGATGGG TGTGTGTCGG
    GCTGATGAGA GTGAAGACTC CCCCGACTCA TGTAAGAACG GGCAGCTGCA GATCCTGTCA
    GACTTTGAAA AAGATGTGGA TCTGGCTTGT CAGACTGTGC TGTTCAAGGA GGTCAACACT
    TGCAACCCAT CCACCATCAC GGGCACGCTG TCCCGCTTGT CTCTGGACGA GGATGAGGAG
    CCCAAGTCCT GCCTCGTGGG CCCCGAGGGC AGCCTCAGCT TCTCACCACT GCCTGGCAAC
    ATCCTGGTGC CCATGGCAGC CTCACCAGGG CTGGGGGAGC CACCGCCCCC GCAGGAGGCC
    AACCCCGTGT ACATGTGTGG AGAGGGCGGC CTGCGGCAGC TGGACCTCAC ATGGCTGCGG
    CCGGCCGAGC CGGGCTCCGA GGGGGACTAC ATGGTGCTGC CCCGGAGGAC TCTGAGTCTG
    CAGCCTGGCG GCGGGGGTGG AGGTGGCGAG GATCCCCCGC GGGCCCGGCC TGAGGGCACC
    CCCCGGCGGG CCTCCAAGTC CCTGGGCCAC ACCGAAGCCT ACCCCAGCTT CCTGTCTGTG
    GAACACTCGG GCCTGGGGCT GGGCCCTGCC TATGGGTCTC TACAGAACCC CTACGGGATG
    ACCTTCCAAC CGCCGCCGCC AACACCCAGC GCCCGCCAGG TGCCAGAGCC GGGGGAGCGC
    AGCCGGACCA TGCCCCGAAC TGTGCCTGGC TCCACCATGA AGCTGGGCTC CCTGGAGCGA
    AAGAAGTTAC GGTATTCAGA CCTGGACTTT GAGGTGATGC ACACCCGGAA ACGGCACTCG
    GAACTCTACC ACGAGCTCAA CCAGAAGTTC CACACTTTCG ACCGCTACCG CAGCCAGTCC
    ACGGCCAAGG AGAAGCCCAG TCCAGGGGAG CGTCCCGGCT TATCCCAACA GCGGCGACAT
    CAGAGCTGGA GCACCTTCAA GTCTATGACG CTGGGCTCGC TGCCCCCCAA GCCCCGAGAG
    CGGCTGGCCC TGCACCGGGC AACAGCCTGG GAACCCACAG AACCACCGGA CGGCGACTTC
    CAGACGGAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46637
    Clone ID Related Accession (Same CDS sequence) XM_010802505.2
    Accession Version XM_010802505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: adhesion G protein-coupled receptor B2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACGCCA GGAGCAGTGT GTACCCACTT
    CGCCCTCGCC TGCTGCCGCT GNGACACTAC CTGGTCAACT TCACCTGCCT GCGGCCCAGC
    CCCGAGGAGG CAGACGCCCA TGCGGGGTCT GAGCTGGGGC GGCCCGAGGA GGAGAGAAGA
    CGACGAGAAG CCGCGGGGTT GGAGCTCTGT GGTGGCTCTG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCCGAGC CCTCCGAGGC CCCGCGCCTG
    CTGGCGCCCG CGGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGCGG GGTCCTCTGC CGCTGGAGTG AGGAGTGCAG CCGCGCGGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCGGGC TGCAGCTGCC CCGGTGAGGC GGGGGCCGGC
    CCCGCGGCCA CCACCACGCC TCCTGGTCCG CCTGCTGCCC ACACCCTGTC CAATGCCCTG
    GTGCCCGGGG GCCCCGCCCC ACCTGCCGAG GCTGATTTGC ACTCGGGGAG CAGCAATGAT
    CTGTTCACCA CGGAGATGAG ATATGGTGAG GAGCCGGAAG AGGAACCGAA GGTGAAAACC
    CAGTGGCCAA GGTCTGCAGA TGAGCCTGGG CTGTACATGG CACAGACAGG CGACCCGGCA
    GCTGAGGAGT GGTCCCCGTG GAGCGTGTGT TCCCTGACGT GTGGGCAGGG CCTGCAGGTG
    CGGACCCGCT CCTGCGTGTC CTCCCCCTAT GGGACCCTGT GCAGCGGGCC CCTGCGGGAG
    ACCCGGCCCT GCAACAATTC AGCCACCTGC CCAGTGCACG GCGTGTGGGA GGAGTGGGGG
    TCCTGGAGCC TGTGCTCCCG CAGCTGCGGG CGGGGGTCCC GGAGCCGGAT GCGGACCTGC
    GTGCCCCCCC AGCACGGCGG CAAGGCCTGC GAGGGTCCCG AGCTGCAGAC TAAGCTCTGC
    AGTATGGCCG CCTGCCCGGT GGAAGGCCAG TGGCTAGAAT GGGGTCCCTG GGGCCCATGT
    TCCACGTCTT GTGCCAACGG GACCCAGCAG CGCAGCCGGA AGTGCAGCGT GGCAGGCCCA
    GCCTGGGCCA CGTGTGCCGG GGCCCTCACT GACACCCGGG AGTGCGGCAA CCTTGAGTGC
    CCGGCCACAG ATGGCAAGTG GGGGCCATGG AATGCGTGGA GCCTGTGTTC CAAGACATGT
    GACACAGGCT GGCAGCGCCG TTTCCGCATG TGCCAGGCCA CAGGCGCTCA GGGCTACCCC
    TGCGAGGGCA CTGGCGAGGA GGTGAAGCCC TGCAGCGAGA AGAGGTGTCC AGCCTTCCAT
    GAGATGTGCA GGGACGAGTA CGTGATGCTG ATGACATGGA AGAAGGCGGC TGCTGGCGAG
    ATCATCTACA ACAAGTGCCC CCCCAATGCC TCAGGGTCTG CCAGCCGCCG CTGTCTGCTC
    AGTGCCCAGG GTGTGGCCTA CTGGGGGCTG CCCAGCTTTG CCCGCTGCAT CTCCCACGAG
    TACCGCTACC TGTATCTGTC ACTTCGGGAG CACCTGGCCA AGGGGCAGCG CATGCTGGCG
    GGCGAGGGCA TGTCTCAGGT GGTGCGCAGC CTGCAGGAGC TCCTGGCCCG GCGCACTTAC
    TACAGCGGGG ACCTGCTTTT CTCCGTGGAC ATTCTGAGGA ACGTCACCGA CACCTTCAAG
    AGAGCCACCT ACGTGCCCTC GGCTGATGAC GTGCAGCGCT TCTTCCAGGT GGTGAGCTTC
    ATGGTAGATG CAGAGAACAA GGACAAGTGG GATGACGCTC AGCAGGTGTC CCCAGGCTCT
    GTGCACCTGC TCCGGGTGGT GGAAGACTTC ATTCACCTGG TGGGCGATGC TCTCAAGGCC
    TTCCAGAGCT CTCTGATTGT CACAGATAAC CTGGTGATCA GCATTCAGCG AGAGCCTGTC
    TCCGCTGTTT CCAGCGACAT CACGTTCCCC ATGCGGGGCC GCCGGGGCAT GAAGGACTGG
    GTGCGGTACT CAGAGGACCG CCTCTTCCTA CCCAAGGAGG TGCTCAGCCT CTCCTCCCCA
    GGGAAGCCGG CCGCATCTGG GGCAGCGGGC AGCCTGGGCA GAGGGAGAGG CCCCGGAACC
    GTGCCCCCTG GCCCCGGCCA CTCCCACCAG CGCCTCCTGC CGGCAGACCC TGAGGATTCC
    TCCTCCTACT TTGTGATTGG TGCTGTGCTC TACCGCACCC TGGGCCTCAT CCTGCCACCA
    CCCAGGCCCC CGCTGGCTGT CACATCCAGA GTGATGACAG TGACCGTAAG GCCTCCCACC
    CAGCCACTAG CTGAGCCCCT CATCACAGTG GAGCTTTCCT ACATCATCAA TGGCACCACA
    GATCCCCATT GTGCCAGCTG GGACTACTCC AGAGCAGACG CCAGCTCAGG GGACTGGGAT
    ACCGAGAGCT GCCAGACGCT GGAGACACAG GCAGCCCACA CCCGCTGCCA GTGCCAGCGC
    CTGTCCACCT TCGCCGTGCT GGCCCAGCCG CCCAAGGACC TGACCCTGGA GCTGGCGGGC
    GCCCCCTCGG TCCCCCTTGT GATCGGCTGT GCTGTGTCCT GCATGGCACT GCTCACCCTG
    CTTGCCATCT ACGCCGCCTT CTGGAGGTTC ATAAAATCTG AACGCTCCAT CATCTTGCTG
    AACTTCTGCC TGTCCATCCT GGCATCCAAC ATCCTCATCC TTGTGGGCCA GTCGCGGGTG
    CTGAGCAAGG GTGTGTGTAC CATGACCGCC GCCTTCCTGC ACTTCTTCTT CCTGTCGTCC
    TTCTGCTGGG TGCTCACTGA GGCCTGGCAG TCCTACCTGG CTGTCATCGG GCGGATGCGC
    ACCCGCCTTG TCCGCAAGCG CTTCCTCTGC CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG
    GCCGTGTCCG TCGGCTTTAC CCGCACCAAA GGATACGGTA CATCCAGCTA CTGCTGGCTC
    TCTCTGGAGG GTGGCCTGCT TTATGCCTTC GTGGGCCCAG CAGCTGTAAT TGTCCTGGTG
    AATATGCTCA TCGGAATCAT CGTCTTCAAC AAGCTCATGG CTCGCGATGG CATCTCAGAC
    AAGTCCAAGA AGCAGAGGGC CGGGTCGGAG CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC
    TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC CTGCTCAGCT CAGCCTCGGC CAGGAACGCC
    ATGGCCTCAC TCTGGAGCTC CTGTGTGGTG CTGCCCCTGC TGGCGCTCAC CTGGATGTCT
    GCCGTCCTGG CCATGACAGA CCGCCGCTCC GTCCTCTTCC AGGCCCTGTT CGCGGTCTTC
    AACTCTGCTC AGGGCTTTGT CATCACGGCC GTCCACTGCT TCCTGCGCCG AGAGGTCCAG
    GACGTGGTGA AGTGCCAGAT GGGTGTGTGT CGGGCTGATG AGAGTGAAGA CTCCCCCGAC
    TCATGTAAGA ACGGGCAGCT GCAGATCCTG TCAGACTTTG AAAAAGATGT GGATCTGGCT
    TGTCAGACTG TGCTGTTCAA GGAGGTCAAC ACTTGCAACC CATCCACCAT CACGGGCACG
    CTGTCCCGCT TGTCTCTGGA CGAGGATGAG GAGCCCAAGT CCTGCCTCGT GGGCCCCGAG
    GGCAGCCTCA GCTTCTCACC ACTGCCTGGC AACATCCTGG TGCCCATGGC AGCCTCACCA
    GGGCTGGGGG AGCCACCGCC CCCGCAGGAG GCCAACCCCG TGTACATGTG TGGAGAGGGC
    GGCCTGCGGC AGCTGGACCT CACATGGCTG CGGCCGGCCG AGCCGGGCTC CGAGGGGGAC
    TACATGGTGC TGCCCCGGAG GACTCTGAGT CTGCAGCCTG GCGGCGGGGG TGGAGGTGGC
    GAGGATCCCC CGCGGGCCCG GCCTGAGGGC ACCCCCCGGC GGGCCTCCAA GTCCCTGGGC
    CACACCGAAG CCTACCCCAG CTTCCTGTCT GTGGAACACT CGGGCCTGGG GCTGGGCCCT
    GCCTATGGGT CTCTACAGAA CCCCTACGGG ATGACCTTCC AACCGCCGCC GCCAACACCC
    AGCGCCCGCC AGGTGCCAGA GCCGGGGGAG CGCAGCCGGA CCATGCCCCG AACTGTGCCT
    GGCTCCACCA TGAAGCTGGG CTCCCTGGAG CGAAAGAAGT TACGGTATTC AGACCTGGAC
    TTTGAGGTGA TGCACACCCG GAAACGGCAC TCGGAACTCT ACCACGAGCT CAACCAGAAG
    TTCCACACTT TCGACCGCTA CCGCAGCCAG TCCACGGCCA AGGAGAAGCC CAGTCCAGGG
    GAGCGTCCCG GCTTATCCCA ACAGCGGCGA CATCAGAGCT GGAGCACCTT CAAGTCTATG
    ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA GAGCGGCTGG CCCTGCACCG GGCAACAGCC
    TGGGAACCCA CAGAACCACC GGACGGCGAC TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800807.1
    CDS132..4799
    Translation

    Target ORF information:

    RefSeq Version XM_010802505.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGACCCCAG CCTGTCCCCT CTTACTATCT GTGATTCTGT CCCTGCGCCT GGCCGCCGCC 
    TTCGACCCCG CCCCCAGCGC CTGCTCCGCC CTGGCCTCAG GCGTGCTTTA CGGGGCCTTC
    TCACTGCAGG ACCTCTTTCC TACCATCGCC TCGGGCTGCT CCTGGACCTT GGAGAACCCA
    GACCCTACCA AGTACTCCCT CTACCTGCGC TTCAACGCCA GGAGCAGTGT GTACCCACTT
    CGCCCTCGCC TGCTGCCGCT GNGACACTAC CTGGTCAACT TCACCTGCCT GCGGCCCAGC
    CCCGAGGAGG CAGACGCCCA TGCGGGGTCT GAGCTGGGGC GGCCCGAGGA GGAGAGAAGA
    CGACGAGAAG CCGCGGGGTT GGAGCTCTGT GGTGGCTCTG GCCCCTTCAC CTTCCTGCAC
    TTCGACAAGA ACTTCGTGCA GCTGTGCCTG TCGGCCGAGC CCTCCGAGGC CCCGCGCCTG
    CTGGCGCCCG CGGCCCTGGC CTTCCGCTTC GTCGAGGTCT TGCTCATCAA CAACAACAAC
    TCCAGCCAGT TCACCTGCGG GGTCCTCTGC CGCTGGAGTG AGGAGTGCAG CCGCGCGGCC
    GGCAGGGCCT GTGGCTTCGC CCAGCCGGGC TGCAGCTGCC CCGGTGAGGC GGGGGCCGGC
    CCCGCGGCCA CCACCACGCC TCCTGGTCCG CCTGCTGCCC ACACCCTGTC CAATGCCCTG
    GTGCCCGGGG GCCCCGCCCC ACCTGCCGAG GCTGATTTGC ACTCGGGGAG CAGCAATGAT
    CTGTTCACCA CGGAGATGAG ATATGGTGAG GAGCCGGAAG AGGAACCGAA GGTGAAAACC
    CAGTGGCCAA GGTCTGCAGA TGAGCCTGGG CTGTACATGG CACAGACAGG CGACCCGGCA
    GCTGAGGAGT GGTCCCCGTG GAGCGTGTGT TCCCTGACGT GTGGGCAGGG CCTGCAGGTG
    CGGACCCGCT CCTGCGTGTC CTCCCCCTAT GGGACCCTGT GCAGCGGGCC CCTGCGGGAG
    ACCCGGCCCT GCAACAATTC AGCCACCTGC CCAGTGCACG GCGTGTGGGA GGAGTGGGGG
    TCCTGGAGCC TGTGCTCCCG CAGCTGCGGG CGGGGGTCCC GGAGCCGGAT GCGGACCTGC
    GTGCCCCCCC AGCACGGCGG CAAGGCCTGC GAGGGTCCCG AGCTGCAGAC TAAGCTCTGC
    AGTATGGCCG CCTGCCCGGT GGAAGGCCAG TGGCTAGAAT GGGGTCCCTG GGGCCCATGT
    TCCACGTCTT GTGCCAACGG GACCCAGCAG CGCAGCCGGA AGTGCAGCGT GGCAGGCCCA
    GCCTGGGCCA CGTGTGCCGG GGCCCTCACT GACACCCGGG AGTGCGGCAA CCTTGAGTGC
    CCGGCCACAG ATGGCAAGTG GGGGCCATGG AATGCGTGGA GCCTGTGTTC CAAGACATGT
    GACACAGGCT GGCAGCGCCG TTTCCGCATG TGCCAGGCCA CAGGCGCTCA GGGCTACCCC
    TGCGAGGGCA CTGGCGAGGA GGTGAAGCCC TGCAGCGAGA AGAGGTGTCC AGCCTTCCAT
    GAGATGTGCA GGGACGAGTA CGTGATGCTG ATGACATGGA AGAAGGCGGC TGCTGGCGAG
    ATCATCTACA ACAAGTGCCC CCCCAATGCC TCAGGGTCTG CCAGCCGCCG CTGTCTGCTC
    AGTGCCCAGG GTGTGGCCTA CTGGGGGCTG CCCAGCTTTG CCCGCTGCAT CTCCCACGAG
    TACCGCTACC TGTATCTGTC ACTTCGGGAG CACCTGGCCA AGGGGCAGCG CATGCTGGCG
    GGCGAGGGCA TGTCTCAGGT GGTGCGCAGC CTGCAGGAGC TCCTGGCCCG GCGCACTTAC
    TACAGCGGGG ACCTGCTTTT CTCCGTGGAC ATTCTGAGGA ACGTCACCGA CACCTTCAAG
    AGAGCCACCT ACGTGCCCTC GGCTGATGAC GTGCAGCGCT TCTTCCAGGT GGTGAGCTTC
    ATGGTAGATG CAGAGAACAA GGACAAGTGG GATGACGCTC AGCAGGTGTC CCCAGGCTCT
    GTGCACCTGC TCCGGGTGGT GGAAGACTTC ATTCACCTGG TGGGCGATGC TCTCAAGGCC
    TTCCAGAGCT CTCTGATTGT CACAGATAAC CTGGTGATCA GCATTCAGCG AGAGCCTGTC
    TCCGCTGTTT CCAGCGACAT CACGTTCCCC ATGCGGGGCC GCCGGGGCAT GAAGGACTGG
    GTGCGGTACT CAGAGGACCG CCTCTTCCTA CCCAAGGAGG TGCTCAGCCT CTCCTCCCCA
    GGGAAGCCGG CCGCATCTGG GGCAGCGGGC AGCCTGGGCA GAGGGAGAGG CCCCGGAACC
    GTGCCCCCTG GCCCCGGCCA CTCCCACCAG CGCCTCCTGC CGGCAGACCC TGAGGATTCC
    TCCTCCTACT TTGTGATTGG TGCTGTGCTC TACCGCACCC TGGGCCTCAT CCTGCCACCA
    CCCAGGCCCC CGCTGGCTGT CACATCCAGA GTGATGACAG TGACCGTAAG GCCTCCCACC
    CAGCCACTAG CTGAGCCCCT CATCACAGTG GAGCTTTCCT ACATCATCAA TGGCACCACA
    GATCCCCATT GTGCCAGCTG GGACTACTCC AGAGCAGACG CCAGCTCAGG GGACTGGGAT
    ACCGAGAGCT GCCAGACGCT GGAGACACAG GCAGCCCACA CCCGCTGCCA GTGCCAGCGC
    CTGTCCACCT TCGCCGTGCT GGCCCAGCCG CCCAAGGACC TGACCCTGGA GCTGGCGGGC
    GCCCCCTCGG TCCCCCTTGT GATCGGCTGT GCTGTGTCCT GCATGGCACT GCTCACCCTG
    CTTGCCATCT ACGCCGCCTT CTGGAGGTTC ATAAAATCTG AACGCTCCAT CATCTTGCTG
    AACTTCTGCC TGTCCATCCT GGCATCCAAC ATCCTCATCC TTGTGGGCCA GTCGCGGGTG
    CTGAGCAAGG GTGTGTGTAC CATGACCGCC GCCTTCCTGC ACTTCTTCTT CCTGTCGTCC
    TTCTGCTGGG TGCTCACTGA GGCCTGGCAG TCCTACCTGG CTGTCATCGG GCGGATGCGC
    ACCCGCCTTG TCCGCAAGCG CTTCCTCTGC CTGGGCTGGG GTCTGCCTGC CCTGGTGGTG
    GCCGTGTCCG TCGGCTTTAC CCGCACCAAA GGATACGGTA CATCCAGCTA CTGCTGGCTC
    TCTCTGGAGG GTGGCCTGCT TTATGCCTTC GTGGGCCCAG CAGCTGTAAT TGTCCTGGTG
    AATATGCTCA TCGGAATCAT CGTCTTCAAC AAGCTCATGG CTCGCGATGG CATCTCAGAC
    AAGTCCAAGA AGCAGAGGGC CGGGTCGGAG CGGTGCCCCT GGGCCAGCCT GCTCCTCCCC
    TGCTCAGCGT GTGGAGCGGT CCCCAGCCCC CTGCTCAGCT CAGCCTCGGC CAGGAACGCC
    ATGGCCTCAC TCTGGAGCTC CTGTGTGGTG CTGCCCCTGC TGGCGCTCAC CTGGATGTCT
    GCCGTCCTGG CCATGACAGA CCGCCGCTCC GTCCTCTTCC AGGCCCTGTT CGCGGTCTTC
    AACTCTGCTC AGGGCTTTGT CATCACGGCC GTCCACTGCT TCCTGCGCCG AGAGGTCCAG
    GACGTGGTGA AGTGCCAGAT GGGTGTGTGT CGGGCTGATG AGAGTGAAGA CTCCCCCGAC
    TCATGTAAGA ACGGGCAGCT GCAGATCCTG TCAGACTTTG AAAAAGATGT GGATCTGGCT
    TGTCAGACTG TGCTGTTCAA GGAGGTCAAC ACTTGCAACC CATCCACCAT CACGGGCACG
    CTGTCCCGCT TGTCTCTGGA CGAGGATGAG GAGCCCAAGT CCTGCCTCGT GGGCCCCGAG
    GGCAGCCTCA GCTTCTCACC ACTGCCTGGC AACATCCTGG TGCCCATGGC AGCCTCACCA
    GGGCTGGGGG AGCCACCGCC CCCGCAGGAG GCCAACCCCG TGTACATGTG TGGAGAGGGC
    GGCCTGCGGC AGCTGGACCT CACATGGCTG CGGCCGGCCG AGCCGGGCTC CGAGGGGGAC
    TACATGGTGC TGCCCCGGAG GACTCTGAGT CTGCAGCCTG GCGGCGGGGG TGGAGGTGGC
    GAGGATCCCC CGCGGGCCCG GCCTGAGGGC ACCCCCCGGC GGGCCTCCAA GTCCCTGGGC
    CACACCGAAG CCTACCCCAG CTTCCTGTCT GTGGAACACT CGGGCCTGGG GCTGGGCCCT
    GCCTATGGGT CTCTACAGAA CCCCTACGGG ATGACCTTCC AACCGCCGCC GCCAACACCC
    AGCGCCCGCC AGGTGCCAGA GCCGGGGGAG CGCAGCCGGA CCATGCCCCG AACTGTGCCT
    GGCTCCACCA TGAAGCTGGG CTCCCTGGAG CGAAAGAAGT TACGGTATTC AGACCTGGAC
    TTTGAGGTGA TGCACACCCG GAAACGGCAC TCGGAACTCT ACCACGAGCT CAACCAGAAG
    TTCCACACTT TCGACCGCTA CCGCAGCCAG TCCACGGCCA AGGAGAAGCC CAGTCCAGGG
    GAGCGTCCCG GCTTATCCCA ACAGCGGCGA CATCAGAGCT GGAGCACCTT CAAGTCTATG
    ACGCTGGGCT CGCTGCCCCC CAAGCCCCGA GAGCGGCTGG CCCTGCACCG GGCAACAGCC
    TGGGAACCCA CAGAACCACC GGACGGCGAC TTCCAGACGG AGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89513
    Clone ID Related Accession (Same CDS sequence) XM_015462437.1
    Accession Version XM_015462437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: adhesion G protein-coupled receptor B2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCCAGA ACCTAGAACA GCCCACAGCC CAGAGATACC CCGCCAGGAT CCATGACAGC 
    CAGAACACTG AGGTATTTGT CACATGGGCT CTGGGACAGA TCCTGGTAGA AGACTGTCAT
    ATAGAAGACC TCAACTCCAG CCACCCCACT GCCCCTCTCA GTGCCCAAGA GGCGGCGCGC
    GTCGCCCCCA CCCCCCTCCC GCCCCTTCTC CCGCGGCTCC TCGCGCCCAG CCCGGCGCGC
    GCCCCCTCTC GGGCCCGGCC CGCGCAGGCG CCGCGTCCCG CCTCGGCCGT CACTCGGCCC
    GGCGCGCGCT GCGGGCAGCG GCCGGAGCGG AGGCGGCGCG CGGCGCGGGC GGTGCTCAGG
    GCGGGGGAGC GGGGAGCCGG GGGGTTGGGG CGGCGTGGCG AGGAGGGCGG CGGCAGGAGC
    CCGCGCCCGG CCCGGCGCCG CCCGCCCCGC GCGCTCCGCG GCGCCTTTTC TGCACCCGCC
    GCTCCGCGGA GCGGGAGCCT CGGGCTGCCC GCGCGGCTGC AGCTAGCTGC CCCGCGCCCG
    GCCAAGCCCC CGCCCTATGG ATGGCAAACT TGCGCCCCGT GGCCGCCTCC TCCAGCCCCG
    GCCCCCGCTC CTGCTGCTGA CGGCGCCCAG GGCAAGGGAC ATAGGATGAC CCCAGCCTGT
    CCCCTCTTAC TATCTGTGAT TCTGTCCCTG CGCCTGGCCG CCGCCTTCGA CCCCGCCCCC
    AGCGCCTGCT CCGCCCTGGC CTCAGGCGTG CTTTACGGGG CCTTCTCACT GCAGGACCTC
    TTTCCTACCA TCGCCTCGGG CTGCTCCTGG ACCTTGGAGA ACCCAGACCC TACCAAGTAC
    TCCCTCTACC TGCGCTTCAA CGCCAGGAGC AGTGTGTACC CACTTCGCCC TCGCCTGCTG
    CCGCTGNGAC ACTACCTGGT CAACTTCACC TGCCTGCGGC CCAGCCCCGA GGAGGCAGAC
    GCCCATGCGG GGTCTGAGCT GGGGCGGCCC GAGGAGGAGA GAAGACGACG AGAAGCCGCG
    GGGTTGGAGC TCTGTGGTGG CTCTGGCCCC TTCACCTTCC TGCACTTCGA CAAGAACTTC
    GTGCAGCTGT GCCTGTCGGC CGAGCCCTCC GAGGCCCCGC GCCTGCTGGC GCCCGCGGCC
    CTGGCCTTCC GCTTCGTCGA GGTCTTGCTC ATCAACAACA ACAACTCCAG CCAGTTCACC
    TGCGGGGTCC TCTGCCGCTG GAGTGAGGAG TGCAGCCGCG CGGCCGGCAG GGCCTGTGGC
    TTCGCCCAGC CGGGCTGCAG CTGCCCCGGT GAGGCGGGGG CCGGCCCCGC GGCCACCACC
    ACGCCTCCTG GTCCGCCTGC TGCCCACACC CTGTCCAATG CCCTGGTGCC CGGGGGCCCC
    GCCCCACCTG CCGAGGCTGA TTTGCACTCG GGGAGCAGCA ATGATCTGTT CACCACGGAG
    ATGAGATATG GTGAGGAGCC GGAAGAGGAA CCGAAGGTGA AAACCCAGTG GCCAAGGTCT
    GCAGATGAGC CTGGGCTGTA CATGGCACAG ACAGGCGACC CGGCAGCTGA GGAGTGGTCC
    CCGTGGAGCG TGTGTTCCCT GACGTGTGGG CAGGGCCTGC AGGTGCGGAC CCGCTCCTGC
    GTGTCCTCCC CCTATGGGAC CCTGTGCAGC GGGCCCCTGC GGGAGACCCG GCCCTGCAAC
    AATTCAGCCA CCTGCCCAGT GCACGGCGTG TGGGAGGAGT GGGGGTCCTG GAGCCTGTGC
    TCCCGCAGCT GCGGGCGGGG GTCCCGGAGC CGGATGCGGA CCTGCGTGCC CCCCCAGCAC
    GGCGGCAAGG CCTGCGAGGG TCCCGAGCTG CAGACTAAGC TCTGCAGTAT GGCCGCCTGC
    CCGGTGGAAG GCCAGTGGCT AGAATGGGGT CCCTGGGGCC CATGTTCCAC GTCTTGTGCC
    AACGGGACCC AGCAGCGCAG CCGGAAGTGC AGCGTGGCAG GCCCAGCCTG GGCCACGTGT
    GCCGGGGCCC TCACTGACAC CCGGGAGTGC GGCAACCTTG AGTGCCCGGC CACAGATGGC
    AAGTGGGGGC CATGGAATGC GTGGAGCCTG TGTTCCAAGA CATGTGACAC AGGCTGGCAG
    CGCCGTTTCC GCATGTGCCA GGCCACAGGC GCTCAGGGCT ACCCCTGCGA GGGCACTGGC
    GAGGAGGTGA AGCCCTGCAG CGAGAAGAGG TGTCCAGCCT TCCATGAGAT GTGCAGGGAC
    GAGTACGTGA TGCTGATGAC ATGGAAGAAG GCGGCTGCTG GCGAGATCAT CTACAACAAG
    TGCCCCCCCA ATGCCTCAGG GTCTGCCAGC CGCCGCTGTC TGCTCAGTGC CCAGGGTGTG
    GCCTACTGGG GGCTGCCCAG CTTTGCCCGC TGCATCTCCC ACGAGTACCG CTACCTGTAT
    CTGTCACTTC GGGAGCACCT GGCCAAGGGG CAGCGCATGC TGGCGGGCGA GGGCATGTCT
    CAGGTGGTGC GCAGCCTGCA GGAGCTCCTG GCCCGGCGCA CTTACTACAG CGGGGACCTG
    CTTTTCTCCG TGGACATTCT GAGGAACGTC ACCGACACCT TCAAGAGAGC CACCTACGTG
    CCCTCGGCTG ATGACGTGCA GCGCTTCTTC CAGGTGGTGA GCTTCATGGT AGATGCAGAG
    AACAAGGACA AGTGGGATGA CGCTCAGCAG GTGTCCCCAG GCTCTGTGCA CCTGCTCCGG
    GTGGTGGAAG ACTTCATTCA CCTGGTGGGC GATGCTCTCA AGGCCTTCCA GAGCTCTCTG
    ATTGTCACAG ATAACCTGGT GATCAGCATT CAGCGAGAGC CTGTCTCCGC TGTTTCCAGC
    GACATCACGT TCCCCATGCG GGGCCGCCGG GGCATGAAGG ACTGGGTGCG GTACTCAGAG
    GACCGCCTCT TCCTACCCAA GGAGGTGCTC AGCCTCTCCT CCCCAGGGAA GCCGGCCGCA
    TCTGGGGCAG CGGGCAGCCT GGGCAGAGGG AGAGGCCCCG GAACCGTGCC CCCTGGCCCC
    GGCCACTCCC ACCAGCGCCT CCTGCCGGCA GACCCTGAGG ATTCCTCCTC CTACTTTGTG
    ATTGGTGCTG TGCTCTACCG CACCCTGGGC CTCATCCTGC CACCACCCAG GCCCCCGCTG
    GCTGTCACAT CCAGAGTGAT GACAGTGACC GTAAGGCCTC CCACCCAGCC ACTAGCTGAG
    CCCCTCATCA CAGTGGAGCT TTCCTACATC ATCAATGGCA CCACAGATCC CCATTGTGCC
    AGCTGGGACT ACTCCAGAGC AGACGCCAGC TCAGGGGACT GGGATACCGA GAGCTGCCAG
    ACGCTGGAGA CACAGGCAGC CCACACCCGC TGCCAGTGCC AGCGCCTGTC CACCTTCGCC
    GTGCTGGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCGCCCC CTCGGTCCCC
    CTTGTGATCG GCTGTGCTGT GTCCTGCATG GCACTGCTCA CCCTGCTTGC CATCTACGCC
    GCCTTCTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC
    ATCCTGGCAT CCAACATCCT CATCCTTGTG GGCCAGTCGC GGGTGCTGAG CAAGGGTGTG
    TGTACCATGA CCGCCGCCTT CCTGCACTTC TTCTTCCTGT CGTCCTTCTG CTGGGTGCTC
    ACTGAGGCCT GGCAGTCCTA CCTGGCTGTC ATCGGGCGGA TGCGCACCCG CCTTGTCCGC
    AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCCGTCGGC
    TTTACCCGCA CCAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCTCT GGAGGGTGGC
    CTGCTTTATG CCTTCGTGGG CCCAGCAGCT GTAATTGTCC TGGTGAATAT GCTCATCGGA
    ATCATCGTCT TCAACAAGCT CATGGCTCGC GATGGCATCT CAGACAAGTC CAAGAAGCAG
    AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA
    GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG
    AGCTCCTGTG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCCATG
    ACAGACCGCC GCTCCGTCCT CTTCCAGGCC CTGTTCGCGG TCTTCAACTC TGCTCAGGGC
    TTTGTCATCA CGGCCGTCCA CTGCTTCCTG CGCCGAGAGG TCCAGGACGT GGTGAAGTGC
    CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CCGACTCATG TAAGAACGGG
    CAGCTGCAGA TCCTGTCAGA CTTTGAAAAA GATGTGGATC TGGCTTGTCA GACTGTGCTG
    TTCAAGGAGG TCAACACTTG CAACCCATCC ACCATCACGG GCACGCTGTC CCGCTTGTCT
    CTGGACGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CCGAGGGCAG CCTCAGCTTC
    TCACCACTGC CTGGCAACAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCA
    CCGCCCCCGC AGGAGGCCAA CCCCGTGTAC ATGTGTGGAG AGGGCGGCCT GCGGCAGCTG
    GACCTCACAT GGCTGCGGCC GGCCGAGCCG GGCTCCGAGG GGGACTACAT GGTGCTGCCC
    CGGAGGACTC TGAGTCTGCA GCCTGGCGGC GGGGGTGGAG GTGGCGAGGA TCCCCCGCGG
    GCCCGGCCTG AGGGCACCCC CCGGCGGGCC TCCAAGTCCC TGGGCCACAC CGAAGCCTAC
    CCCAGCTTCC TGTCTGTGGA ACACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA
    CAGAACCCCT ACGGGATGAC CTTCCAACCG CCGCCGCCAA CACCCAGCGC CCGCCAGGTG
    CCAGAGCCGG GGGAGCGCAG CCGGACCATG CCCCGAACTG TGCCTGGCTC CACCATGAAG
    CTGGGCTCCC TGGAGCGAAA GAAGTTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG
    CACACCCGGA AACGGCACTC GGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC
    GACCGCTACC GCAGCCAGTC CACGGCCAAG GAGAAGCCCA GTCCAGGGGA GCGTCCCGGC
    TTATCCCAAC AGCGGCGACA TCAGAGCTGG AGCACCTTCA AGTCTATGAC GCTGGGCTCG
    CTGCCCCCCA AGCCCCGAGA GCGGCTGGCC CTGCACCGGG CAACAGCCTG GGAACCCACA
    GAACCACCGG ACGGCGACTT CCAGACGGAG GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317923.1
    CDS1..5316
    Translation

    Target ORF information:

    RefSeq Version XM_015462437.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor B2 (ADGRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCCAGA ACCTAGAACA GCCCACAGCC CAGAGATACC CCGCCAGGAT CCATGACAGC 
    CAGAACACTG AGGTATTTGT CACATGGGCT CTGGGACAGA TCCTGGTAGA AGACTGTCAT
    ATAGAAGACC TCAACTCCAG CCACCCCACT GCCCCTCTCA GTGCCCAAGA GGCGGCGCGC
    GTCGCCCCCA CCCCCCTCCC GCCCCTTCTC CCGCGGCTCC TCGCGCCCAG CCCGGCGCGC
    GCCCCCTCTC GGGCCCGGCC CGCGCAGGCG CCGCGTCCCG CCTCGGCCGT CACTCGGCCC
    GGCGCGCGCT GCGGGCAGCG GCCGGAGCGG AGGCGGCGCG CGGCGCGGGC GGTGCTCAGG
    GCGGGGGAGC GGGGAGCCGG GGGGTTGGGG CGGCGTGGCG AGGAGGGCGG CGGCAGGAGC
    CCGCGCCCGG CCCGGCGCCG CCCGCCCCGC GCGCTCCGCG GCGCCTTTTC TGCACCCGCC
    GCTCCGCGGA GCGGGAGCCT CGGGCTGCCC GCGCGGCTGC AGCTAGCTGC CCCGCGCCCG
    GCCAAGCCCC CGCCCTATGG ATGGCAAACT TGCGCCCCGT GGCCGCCTCC TCCAGCCCCG
    GCCCCCGCTC CTGCTGCTGA CGGCGCCCAG GGCAAGGGAC ATAGGATGAC CCCAGCCTGT
    CCCCTCTTAC TATCTGTGAT TCTGTCCCTG CGCCTGGCCG CCGCCTTCGA CCCCGCCCCC
    AGCGCCTGCT CCGCCCTGGC CTCAGGCGTG CTTTACGGGG CCTTCTCACT GCAGGACCTC
    TTTCCTACCA TCGCCTCGGG CTGCTCCTGG ACCTTGGAGA ACCCAGACCC TACCAAGTAC
    TCCCTCTACC TGCGCTTCAA CGCCAGGAGC AGTGTGTACC CACTTCGCCC TCGCCTGCTG
    CCGCTGNGAC ACTACCTGGT CAACTTCACC TGCCTGCGGC CCAGCCCCGA GGAGGCAGAC
    GCCCATGCGG GGTCTGAGCT GGGGCGGCCC GAGGAGGAGA GAAGACGACG AGAAGCCGCG
    GGGTTGGAGC TCTGTGGTGG CTCTGGCCCC TTCACCTTCC TGCACTTCGA CAAGAACTTC
    GTGCAGCTGT GCCTGTCGGC CGAGCCCTCC GAGGCCCCGC GCCTGCTGGC GCCCGCGGCC
    CTGGCCTTCC GCTTCGTCGA GGTCTTGCTC ATCAACAACA ACAACTCCAG CCAGTTCACC
    TGCGGGGTCC TCTGCCGCTG GAGTGAGGAG TGCAGCCGCG CGGCCGGCAG GGCCTGTGGC
    TTCGCCCAGC CGGGCTGCAG CTGCCCCGGT GAGGCGGGGG CCGGCCCCGC GGCCACCACC
    ACGCCTCCTG GTCCGCCTGC TGCCCACACC CTGTCCAATG CCCTGGTGCC CGGGGGCCCC
    GCCCCACCTG CCGAGGCTGA TTTGCACTCG GGGAGCAGCA ATGATCTGTT CACCACGGAG
    ATGAGATATG GTGAGGAGCC GGAAGAGGAA CCGAAGGTGA AAACCCAGTG GCCAAGGTCT
    GCAGATGAGC CTGGGCTGTA CATGGCACAG ACAGGCGACC CGGCAGCTGA GGAGTGGTCC
    CCGTGGAGCG TGTGTTCCCT GACGTGTGGG CAGGGCCTGC AGGTGCGGAC CCGCTCCTGC
    GTGTCCTCCC CCTATGGGAC CCTGTGCAGC GGGCCCCTGC GGGAGACCCG GCCCTGCAAC
    AATTCAGCCA CCTGCCCAGT GCACGGCGTG TGGGAGGAGT GGGGGTCCTG GAGCCTGTGC
    TCCCGCAGCT GCGGGCGGGG GTCCCGGAGC CGGATGCGGA CCTGCGTGCC CCCCCAGCAC
    GGCGGCAAGG CCTGCGAGGG TCCCGAGCTG CAGACTAAGC TCTGCAGTAT GGCCGCCTGC
    CCGGTGGAAG GCCAGTGGCT AGAATGGGGT CCCTGGGGCC CATGTTCCAC GTCTTGTGCC
    AACGGGACCC AGCAGCGCAG CCGGAAGTGC AGCGTGGCAG GCCCAGCCTG GGCCACGTGT
    GCCGGGGCCC TCACTGACAC CCGGGAGTGC GGCAACCTTG AGTGCCCGGC CACAGATGGC
    AAGTGGGGGC CATGGAATGC GTGGAGCCTG TGTTCCAAGA CATGTGACAC AGGCTGGCAG
    CGCCGTTTCC GCATGTGCCA GGCCACAGGC GCTCAGGGCT ACCCCTGCGA GGGCACTGGC
    GAGGAGGTGA AGCCCTGCAG CGAGAAGAGG TGTCCAGCCT TCCATGAGAT GTGCAGGGAC
    GAGTACGTGA TGCTGATGAC ATGGAAGAAG GCGGCTGCTG GCGAGATCAT CTACAACAAG
    TGCCCCCCCA ATGCCTCAGG GTCTGCCAGC CGCCGCTGTC TGCTCAGTGC CCAGGGTGTG
    GCCTACTGGG GGCTGCCCAG CTTTGCCCGC TGCATCTCCC ACGAGTACCG CTACCTGTAT
    CTGTCACTTC GGGAGCACCT GGCCAAGGGG CAGCGCATGC TGGCGGGCGA GGGCATGTCT
    CAGGTGGTGC GCAGCCTGCA GGAGCTCCTG GCCCGGCGCA CTTACTACAG CGGGGACCTG
    CTTTTCTCCG TGGACATTCT GAGGAACGTC ACCGACACCT TCAAGAGAGC CACCTACGTG
    CCCTCGGCTG ATGACGTGCA GCGCTTCTTC CAGGTGGTGA GCTTCATGGT AGATGCAGAG
    AACAAGGACA AGTGGGATGA CGCTCAGCAG GTGTCCCCAG GCTCTGTGCA CCTGCTCCGG
    GTGGTGGAAG ACTTCATTCA CCTGGTGGGC GATGCTCTCA AGGCCTTCCA GAGCTCTCTG
    ATTGTCACAG ATAACCTGGT GATCAGCATT CAGCGAGAGC CTGTCTCCGC TGTTTCCAGC
    GACATCACGT TCCCCATGCG GGGCCGCCGG GGCATGAAGG ACTGGGTGCG GTACTCAGAG
    GACCGCCTCT TCCTACCCAA GGAGGTGCTC AGCCTCTCCT CCCCAGGGAA GCCGGCCGCA
    TCTGGGGCAG CGGGCAGCCT GGGCAGAGGG AGAGGCCCCG GAACCGTGCC CCCTGGCCCC
    GGCCACTCCC ACCAGCGCCT CCTGCCGGCA GACCCTGAGG ATTCCTCCTC CTACTTTGTG
    ATTGGTGCTG TGCTCTACCG CACCCTGGGC CTCATCCTGC CACCACCCAG GCCCCCGCTG
    GCTGTCACAT CCAGAGTGAT GACAGTGACC GTAAGGCCTC CCACCCAGCC ACTAGCTGAG
    CCCCTCATCA CAGTGGAGCT TTCCTACATC ATCAATGGCA CCACAGATCC CCATTGTGCC
    AGCTGGGACT ACTCCAGAGC AGACGCCAGC TCAGGGGACT GGGATACCGA GAGCTGCCAG
    ACGCTGGAGA CACAGGCAGC CCACACCCGC TGCCAGTGCC AGCGCCTGTC CACCTTCGCC
    GTGCTGGCCC AGCCGCCCAA GGACCTGACC CTGGAGCTGG CGGGCGCCCC CTCGGTCCCC
    CTTGTGATCG GCTGTGCTGT GTCCTGCATG GCACTGCTCA CCCTGCTTGC CATCTACGCC
    GCCTTCTGGA GGTTCATAAA ATCTGAACGC TCCATCATCT TGCTGAACTT CTGCCTGTCC
    ATCCTGGCAT CCAACATCCT CATCCTTGTG GGCCAGTCGC GGGTGCTGAG CAAGGGTGTG
    TGTACCATGA CCGCCGCCTT CCTGCACTTC TTCTTCCTGT CGTCCTTCTG CTGGGTGCTC
    ACTGAGGCCT GGCAGTCCTA CCTGGCTGTC ATCGGGCGGA TGCGCACCCG CCTTGTCCGC
    AAGCGCTTCC TCTGCCTGGG CTGGGGTCTG CCTGCCCTGG TGGTGGCCGT GTCCGTCGGC
    TTTACCCGCA CCAAAGGATA CGGTACATCC AGCTACTGCT GGCTCTCTCT GGAGGGTGGC
    CTGCTTTATG CCTTCGTGGG CCCAGCAGCT GTAATTGTCC TGGTGAATAT GCTCATCGGA
    ATCATCGTCT TCAACAAGCT CATGGCTCGC GATGGCATCT CAGACAAGTC CAAGAAGCAG
    AGGGCCGGGT CGGAGCGGTG CCCCTGGGCC AGCCTGCTCC TCCCCTGCTC AGCGTGTGGA
    GCGGTCCCCA GCCCCCTGCT CAGCTCAGCC TCGGCCAGGA ACGCCATGGC CTCACTCTGG
    AGCTCCTGTG TGGTGCTGCC CCTGCTGGCG CTCACCTGGA TGTCTGCCGT CCTGGCCATG
    ACAGACCGCC GCTCCGTCCT CTTCCAGGCC CTGTTCGCGG TCTTCAACTC TGCTCAGGGC
    TTTGTCATCA CGGCCGTCCA CTGCTTCCTG CGCCGAGAGG TCCAGGACGT GGTGAAGTGC
    CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CCGACTCATG TAAGAACGGG
    CAGCTGCAGA TCCTGTCAGA CTTTGAAAAA GATGTGGATC TGGCTTGTCA GACTGTGCTG
    TTCAAGGAGG TCAACACTTG CAACCCATCC ACCATCACGG GCACGCTGTC CCGCTTGTCT
    CTGGACGAGG ATGAGGAGCC CAAGTCCTGC CTCGTGGGCC CCGAGGGCAG CCTCAGCTTC
    TCACCACTGC CTGGCAACAT CCTGGTGCCC ATGGCAGCCT CACCAGGGCT GGGGGAGCCA
    CCGCCCCCGC AGGAGGCCAA CCCCGTGTAC ATGTGTGGAG AGGGCGGCCT GCGGCAGCTG
    GACCTCACAT GGCTGCGGCC GGCCGAGCCG GGCTCCGAGG GGGACTACAT GGTGCTGCCC
    CGGAGGACTC TGAGTCTGCA GCCTGGCGGC GGGGGTGGAG GTGGCGAGGA TCCCCCGCGG
    GCCCGGCCTG AGGGCACCCC CCGGCGGGCC TCCAAGTCCC TGGGCCACAC CGAAGCCTAC
    CCCAGCTTCC TGTCTGTGGA ACACTCGGGC CTGGGGCTGG GCCCTGCCTA TGGGTCTCTA
    CAGAACCCCT ACGGGATGAC CTTCCAACCG CCGCCGCCAA CACCCAGCGC CCGCCAGGTG
    CCAGAGCCGG GGGAGCGCAG CCGGACCATG CCCCGAACTG TGCCTGGCTC CACCATGAAG
    CTGGGCTCCC TGGAGCGAAA GAAGTTACGG TATTCAGACC TGGACTTTGA GAAGGTGATG
    CACACCCGGA AACGGCACTC GGAACTCTAC CACGAGCTCA ACCAGAAGTT CCACACTTTC
    GACCGCTACC GCAGCCAGTC CACGGCCAAG GAGAAGCCCA GTCCAGGGGA GCGTCCCGGC
    TTATCCCAAC AGCGGCGACA TCAGAGCTGG AGCACCTTCA AGTCTATGAC GCTGGGCTCG
    CTGCCCCCCA AGCCCCGAGA GCGGCTGGCC CTGCACCGGG CAACAGCCTG GGAACCCACA
    GAACCACCGG ACGGCGACTT CCAGACGGAG GTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.