PHRF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHRF1
Entrez Gene ID	483405
Full Name	PHD and ring finger domains 1
General protein information	Preferred Names PHD and ring finger domains 1 Names PHD and RING finger domain-containing protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014121209.2	XP_013976684.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_014121208.1	XP_013976683.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_014121209.1	XP_013976684.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_005631089.2	XP_005631146.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_014121208.2	XP_013976683.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_022405287.1	XP_022260995.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_005631089.3	XP_005631146.1	PHD and RING finger domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHRF1	XP_508199.3
Mus musculus (house mouse)	Phrf1	NP_001074587.1
Homo sapiens (human)	PHRF1	NP_001273510.1
	PHRF1	XP_001086134.2
Canis lupus familiaris (dog)	PHRF1	XP_540525.3
Rattus norvegicus (Norway rat)	Phrf1	NP_620793.1
Gallus gallus (chicken)	PHRF1	XP_004941520.1

Gene Ontology

The following PHRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe41258	XM_014121209.2 Latest version!	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe19847	XM_005631089.3 Latest version!	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe19847	XM_014121208.2 Latest version!	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe19847	XM_022405287.1 Latest version!	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe41258	XM_014121209.1	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe19847	XM_014121208.1	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe19847	XM_005631089.2	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHRF1 ( XM_014121209.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976684.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe41258
Clone ID Related Accession (Same CDS sequence)	XM_014121209.2 , XM_014121209.1
Accession Version	XM_014121209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGT 
GAGGACCAGT CTACAAACCC TGCTTCCCCT TTGAGCGCCA AGAGGAGGGT TCTGTCCCAG 
TCAGCACTGC GTTCTCACCG GCCCGTGGCC AGGCCTGTGG CCATGGGGCT CTCCAGGAGG 
AGTGTTCCTG CTGCGGCGCC TGAGCCAGAA GTGGATGAGG CCCCGGTCCC TGACCTGGTG 
GGGAGTATCC TTTCGGGCCA GAGCCTCCTC ATGATGAACG GCGCCGACAT CATCATCCAT 
CGGGACGGCT CTCTCAGCGC CAAGAGAGCA GCACCTGTTT CGTTCCCGCG GAGCGCAGGC 
AAGAGCACAG GCGGCTCATC GCAAGAGGGC AAGCACCCTG GGCCCAGGGC CTGCCTGCAG 
CCTAGAGCCC CGTGCGCGGG AAGCGGGTTG CAGAGCATGG GGCTGAGCTG TCGCAGTGGG 
CCCGCACCTT CCCGTGTCCC TGTGCTCTCA ACAGGGGCGA CCGTGAAACT GGACTCGTCG 
GTAACCCCTC CGTCAAGTCA GGCTCAGAAC CCATCAGGCG TGCGTGGGCC TGGCCTAAAG 
CAGAGGGACG ACCCCCGATG TGATGGCGAC AGGCCCACTC TGCCCGCCAG CGCCACACCC 
TCCAAGATCT CTGGTAGCTG CTCTCACTTG CCGCCGGCTG GTGCGCTGCT GGGCCAGGCC 
CTGAAGCCAG CTCCCAGGAG GCCTGACATA TCTGAGCTAC CCAGAATACC AAAGGTCAGA 
AGGGAGGACT GTGGCGGCAG ACGGTCGGAT GCGGCCCCCA CCAGTGAGCA GCGCGTCGAG 
ATCCCCAGCT CCTGCATCAG CCGCCTCACG GGCAGGGAGG GCCCTGGCCA GCCTGGCCAT 
GGTGCCCGAG CAGAGGGCGA GCCCAGCAGC AGGGGCCCAC AAGAGCCCCG GCCACACTCA 
GGGGGCGGCT CCCAGTCCCC CGCCTCCCTG GGGCCCTCGA AGGGGAAGGG TGTCAGCTCG 
ACGTTCGAGA GCTTCAGGAT CAATATTCCT GGGAACACCG CGCATTCCGG GAGACTCTCC 
AACCCTGGCT TTTGTAACAC ATTCCGGCCT GTCGACAACA AGGTGCAGAG GAAAGAGAAC 
CCCTCGCCCC TCTTCTCCAT CAGGAAGACC AAACAGCTCA AGAGTGAGAT CTACGACCCT 
TTTGACCCTA CAGGCTCCGA CTCCAGTTCT GCCGGCAGCA GCCCCGAGCG CCTGGGCTCT 
GGTCTCCTAC CCTCCGAGAT CACTCGCACC ATCTCCATCA ACAGCCCAAA GGCCCCAACA 
TTGCAGACTG TGCGCTGTGT AACCTCCTAC ACAGTGGAGA CAGTCTTCGG GGCCGAGCCC 
GAGCCCCCGA GGAGGCCTTC TTCCGGTGTG CTCGAGCTCC GGGGCGAGGG GGCTCCCGAG 
GAGGCCTCCG ACCTGAAGCG AGAGGGGCTG GGCCAGGGGG CTGCTGAGGG CCGGGGCTCG 
GCCTCCCGGG CGCAGAGGCC GTCCCCATCC GAGCCGTGGG AGGACAAGGA CCAGCTACCC 
TGCAGTACTT TCTTTGGCTC CGAGGAGCGG ACAGTGACCT GCGTGACTGT GGCAGAGCCA 
GAGGCCCCAC CCAGCCCGGA CGCGCCACAG ACAACCACCC ACAGAATCGT GGAGCTCAGG 
TCCCCGTCTT GCTCCCGCTC CACGTCCAGC TCCCGTGGCA GGAAGAAAGC CAAGAGGAAG 
CGGGCCTCTG CCAGGGAGCA CCGGAGGACC CGCTCGGGCT CCCACTCGGG CTCCCGCTCT 
GGGGACAGGA GCTCACGGTC CGTGTCTCCA CCGGTGGGGG AGGACCACCC CAAGAGGCAG 
CAGACCAAGT CCCGGGGCCG GAGGTCTTCT AGTGACCACT CCAGCAGCCA CGAGCGAGCT 
AAGCGGAAGA AGGCGAAGGA GGAGGGCAGG AGGAGGAAGA GGGACTCCTG GGGTCACGGC 
CGGCGGAGGT CCAGGTCCCG CTCAGGCAGC CCGGGGAGCT CGTCCCATGA ACGCAGGGAG 
AGCAGGAGAA GGAAGCGGAG GCGGTCGGGG TCCAGGTCTC GGGGGAGGGA GTGCTCACCC 
GCCAGCAGCC TGGACAGAGC CCGGAGACAC CGGCACCCGA GGGAGAGGAG CCGGGAGCGG 
CCCCGCATCA GGTGGGGCTC CCACGAGAGG AGGAAGCGGA GGTCCAGGTC ACCGAGGTCG 
CCAAGCACGG AGCACAGGTC CCGGGAGCAC CAGCGGCCTC GTTCCCATGA GAAGCGGCCA 
AGGCCTCGTT CCCCGGAGAG GAAGTTGGCA GCTGCTCCCC AGGAGGAGCA GAAGCCTGAC 
AGGGAGCAGC CAGCCAGGCC ACCGGCCTCA GTGGGAGCAG ATGCCTCACC GGCGGGGGCT 
GAGACTCACA AGGTGGCTGT GGAGGTCCCA GCTGAGTGTC CGCCCGAGGA CCTTGATTAC 
GGTGACTCTG TGGAGGCGGG GCACATCTTT GAGGATTTTT CAAGCGAAGT CTTCCTCATG 
CAGCTTGATG ACATGAGCTC CCCGCCCTCC CCGGAGAGCA CGGACTCCTC CCCAGAGCGA 
GACTTCCCAC CCAACCCTGC CGTGCCGCCC GCCAGCCAGC AGCAGGACGC CAGCCTGGCC 
GTGGCTGTCA TCCGACGGGA GGTGTCCCTG ATCCATGGCG AAGATGCCGC ACAGCCCCCG 
CCCCAGGCGC AGGGCCCCGA CGAGAAGCCC CTGCTCCAGC AGGATGCAGC TGGGGCCGGC 
TCAGTGCCCA GCGCCCTGGG GAGCCAGGCT GTGGGCGGGG TGCCCGTGGG AAAGGAGGAA 
GGCCCCTCGC AGACCCCCTT GCTGCGGGCC AAAGCCCTGG TGAAGAGAGT CACCTGGAAC 
CTCCAGGACG CGGAGAGCAG CGCCCCTGCC GAGGACCGAG GCCTGCGGAC GCCGCTTCAC 
AGGCCTCAGA AGCCCCGGGA AGGGGTCTGG GAAGCAGAGG ACATGGGCCC CTCGGTGGGC 
TTCCAGCAGA CATCCTTCTC TGAGCCCCCT CCCCCCGGCT ATGCGCTTCC AGAGTCTGGC 
TTCCCTGACG CTGAGCCCTC TCAGGTTTAT ACGTCCAACC TGCCTCCCGC CCCAGCTCTG 
CCCGTGAGCC TCCCACCCTA CGCACCGGCC AGCCAGCCCA CAGTGCAGTT CATCCTGCAG 
GGGAGCCTGC CCCTGGCCAG CTGTGGGGTT GCACAGAGCC CGGCCCCCGT GCCCACCTCC 
CTGGCCACGG CCTCGGAGCC AGCCGGCCAC TCCACTGGTG CCAGCAACTC CGAGGAGAGG 
ACGGCCGCTC CCAGGCCTGC CGCAGAGAAG GCCAAGAATG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGGGCAGT GGAGGAAGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CGAAGGACGA GTACAAGGAC ATCCTCCGCA AGGCGGTACA GAAGATATGC 
CACAGCAAGA GCGGGGAGAT CAACCCCGTG AAGGTGGGCA ACCTGGTCAA GGCCTATGTG 
GACAAATACA GACACATGCG CAGGCACCGC AGGGCCGAGG CTGGCGAGGA GGCGCCCGCC 
CAGGGCCCTG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976684.1
CDS	224..5101
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_014121209.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121209.2

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGT 
GAGGACCAGT CTACAAACCC TGCTTCCCCT TTGAGCGCCA AGAGGAGGGT TCTGTCCCAG 
TCAGCACTGC GTTCTCACCG GCCCGTGGCC AGGCCTGTGG CCATGGGGCT CTCCAGGAGG 
AGTGTTCCTG CTGCGGCGCC TGAGCCAGAA GTGGATGAGG CCCCGGTCCC TGACCTGGTG 
GGGAGTATCC TTTCGGGCCA GAGCCTCCTC ATGATGAACG GCGCCGACAT CATCATCCAT 
CGGGACGGCT CTCTCAGCGC CAAGAGAGCA GCACCTGTTT CGTTCCCGCG GAGCGCAGGC 
AAGAGCACAG GCGGCTCATC GCAAGAGGGC AAGCACCCTG GGCCCAGGGC CTGCCTGCAG 
CCTAGAGCCC CGTGCGCGGG AAGCGGGTTG CAGAGCATGG GGCTGAGCTG TCGCAGTGGG 
CCCGCACCTT CCCGTGTCCC TGTGCTCTCA ACAGGGGCGA CCGTGAAACT GGACTCGTCG 
GTAACCCCTC CGTCAAGTCA GGCTCAGAAC CCATCAGGCG TGCGTGGGCC TGGCCTAAAG 
CAGAGGGACG ACCCCCGATG TGATGGCGAC AGGCCCACTC TGCCCGCCAG CGCCACACCC 
TCCAAGATCT CTGGTAGCTG CTCTCACTTG CCGCCGGCTG GTGCGCTGCT GGGCCAGGCC 
CTGAAGCCAG CTCCCAGGAG GCCTGACATA TCTGAGCTAC CCAGAATACC AAAGGTCAGA 
AGGGAGGACT GTGGCGGCAG ACGGTCGGAT GCGGCCCCCA CCAGTGAGCA GCGCGTCGAG 
ATCCCCAGCT CCTGCATCAG CCGCCTCACG GGCAGGGAGG GCCCTGGCCA GCCTGGCCAT 
GGTGCCCGAG CAGAGGGCGA GCCCAGCAGC AGGGGCCCAC AAGAGCCCCG GCCACACTCA 
GGGGGCGGCT CCCAGTCCCC CGCCTCCCTG GGGCCCTCGA AGGGGAAGGG TGTCAGCTCG 
ACGTTCGAGA GCTTCAGGAT CAATATTCCT GGGAACACCG CGCATTCCGG GAGACTCTCC 
AACCCTGGCT TTTGTAACAC ATTCCGGCCT GTCGACAACA AGGTGCAGAG GAAAGAGAAC 
CCCTCGCCCC TCTTCTCCAT CAGGAAGACC AAACAGCTCA AGAGTGAGAT CTACGACCCT 
TTTGACCCTA CAGGCTCCGA CTCCAGTTCT GCCGGCAGCA GCCCCGAGCG CCTGGGCTCT 
GGTCTCCTAC CCTCCGAGAT CACTCGCACC ATCTCCATCA ACAGCCCAAA GGCCCCAACA 
TTGCAGACTG TGCGCTGTGT AACCTCCTAC ACAGTGGAGA CAGTCTTCGG GGCCGAGCCC 
GAGCCCCCGA GGAGGCCTTC TTCCGGTGTG CTCGAGCTCC GGGGCGAGGG GGCTCCCGAG 
GAGGCCTCCG ACCTGAAGCG AGAGGGGCTG GGCCAGGGGG CTGCTGAGGG CCGGGGCTCG 
GCCTCCCGGG CGCAGAGGCC GTCCCCATCC GAGCCGTGGG AGGACAAGGA CCAGCTACCC 
TGCAGTACTT TCTTTGGCTC CGAGGAGCGG ACAGTGACCT GCGTGACTGT GGCAGAGCCA 
GAGGCCCCAC CCAGCCCGGA CGCGCCACAG ACAACCACCC ACAGAATCGT GGAGCTCAGG 
TCCCCGTCTT GCTCCCGCTC CACGTCCAGC TCCCGTGGCA GGAAGAAAGC CAAGAGGAAG 
CGGGCCTCTG CCAGGGAGCA CCGGAGGACC CGCTCGGGCT CCCACTCGGG CTCCCGCTCT 
GGGGACAGGA GCTCACGGTC CGTGTCTCCA CCGGTGGGGG AGGACCACCC CAAGAGGCAG 
CAGACCAAGT CCCGGGGCCG GAGGTCTTCT AGTGACCACT CCAGCAGCCA CGAGCGAGCT 
AAGCGGAAGA AGGCGAAGGA GGAGGGCAGG AGGAGGAAGA GGGACTCCTG GGGTCACGGC 
CGGCGGAGGT CCAGGTCCCG CTCAGGCAGC CCGGGGAGCT CGTCCCATGA ACGCAGGGAG 
AGCAGGAGAA GGAAGCGGAG GCGGTCGGGG TCCAGGTCTC GGGGGAGGGA GTGCTCACCC 
GCCAGCAGCC TGGACAGAGC CCGGAGACAC CGGCACCCGA GGGAGAGGAG CCGGGAGCGG 
CCCCGCATCA GGTGGGGCTC CCACGAGAGG AGGAAGCGGA GGTCCAGGTC ACCGAGGTCG 
CCAAGCACGG AGCACAGGTC CCGGGAGCAC CAGCGGCCTC GTTCCCATGA GAAGCGGCCA 
AGGCCTCGTT CCCCGGAGAG GAAGTTGGCA GCTGCTCCCC AGGAGGAGCA GAAGCCTGAC 
AGGGAGCAGC CAGCCAGGCC ACCGGCCTCA GTGGGAGCAG ATGCCTCACC GGCGGGGGCT 
GAGACTCACA AGGTGGCTGT GGAGGTCCCA GCTGAGTGTC CGCCCGAGGA CCTTGATTAC 
GGTGACTCTG TGGAGGCGGG GCACATCTTT GAGGATTTTT CAAGCGAAGT CTTCCTCATG 
CAGCTTGATG ACATGAGCTC CCCGCCCTCC CCGGAGAGCA CGGACTCCTC CCCAGAGCGA 
GACTTCCCAC CCAACCCTGC CGTGCCGCCC GCCAGCCAGC AGCAGGACGC CAGCCTGGCC 
GTGGCTGTCA TCCGACGGGA GGTGTCCCTG ATCCATGGCG AAGATGCCGC ACAGCCCCCG 
CCCCAGGCGC AGGGCCCCGA CGAGAAGCCC CTGCTCCAGC AGGATGCAGC TGGGGCCGGC 
TCAGTGCCCA GCGCCCTGGG GAGCCAGGCT GTGGGCGGGG TGCCCGTGGG AAAGGAGGAA 
GGCCCCTCGC AGACCCCCTT GCTGCGGGCC AAAGCCCTGG TGAAGAGAGT CACCTGGAAC 
CTCCAGGACG CGGAGAGCAG CGCCCCTGCC GAGGACCGAG GCCTGCGGAC GCCGCTTCAC 
AGGCCTCAGA AGCCCCGGGA AGGGGTCTGG GAAGCAGAGG ACATGGGCCC CTCGGTGGGC 
TTCCAGCAGA CATCCTTCTC TGAGCCCCCT CCCCCCGGCT ATGCGCTTCC AGAGTCTGGC 
TTCCCTGACG CTGAGCCCTC TCAGGTTTAT ACGTCCAACC TGCCTCCCGC CCCAGCTCTG 
CCCGTGAGCC TCCCACCCTA CGCACCGGCC AGCCAGCCCA CAGTGCAGTT CATCCTGCAG 
GGGAGCCTGC CCCTGGCCAG CTGTGGGGTT GCACAGAGCC CGGCCCCCGT GCCCACCTCC 
CTGGCCACGG CCTCGGAGCC AGCCGGCCAC TCCACTGGTG CCAGCAACTC CGAGGAGAGG 
ACGGCCGCTC CCAGGCCTGC CGCAGAGAAG GCCAAGAATG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGGGCAGT GGAGGAAGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CGAAGGACGA GTACAAGGAC ATCCTCCGCA AGGCGGTACA GAAGATATGC 
CACAGCAAGA GCGGGGAGAT CAACCCCGTG AAGGTGGGCA ACCTGGTCAA GGCCTATGTG 
GACAAATACA GACACATGCG CAGGCACCGC AGGGCCGAGG CTGGCGAGGA GGCGCCCGCC 
CAGGGCCCTG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_014121209.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976684.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe41258
Clone ID Related Accession (Same CDS sequence)	XM_014121209.2 , XM_014121209.1
Accession Version	XM_014121209.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGT 
GAGGACCAGT CTACAAACCC TGCTTCCCCT TTGAGCGCCA AGAGGAGGGT TCTGTCCCAG 
TCAGCACTGC GTTCTCACCG GCCCGTGGCC AGGCCTGTGG CCATGGGGCT CTCCAGGAGG 
AGTGTTCCTG CTGCGGCGCC TGAGCCAGAA GTGGATGAGG CCCCGGTCCC TGACCTGGTG 
GGGAGTATCC TTTCGGGCCA GAGCCTCCTC ATGATGAACG GCGCCGACAT CATCATCCAT 
CGGGACGGCT CTCTCAGCGC CAAGAGAGCA GCACCTGTTT CGTTCCCGCG GAGCGCAGGC 
AAGAGCACAG GCGGCTCATC GCAAGAGGGC AAGCACCCTG GGCCCAGGGC CTGCCTGCAG 
CCTAGAGCCC CGTGCGCGGG AAGCGGGTTG CAGAGCATGG GGCTGAGCTG TCGCAGTGGG 
CCCGCACCTT CCCGTGTCCC TGTGCTCTCA ACAGGGGCGA CCGTGAAACT GGACTCGTCG 
GTAACCCCTC CGTCAAGTCA GGCTCAGAAC CCATCAGGCG TGCGTGGGCC TGGCCTAAAG 
CAGAGGGACG ACCCCCGATG TGATGGCGAC AGGCCCACTC TGCCCGCCAG CGCCACACCC 
TCCAAGATCT CTGGTAGCTG CTCTCACTTG CCGCCGGCTG GTGCGCTGCT GGGCCAGGCC 
CTGAAGCCAG CTCCCAGGAG GCCTGACATA TCTGAGCTAC CCAGAATACC AAAGGTCAGA 
AGGGAGGACT GTGGCGGCAG ACGGTCGGAT GCGGCCCCCA CCAGTGAGCA GCGCGTCGAG 
ATCCCCAGCT CCTGCATCAG CCGCCTCACG GGCAGGGAGG GCCCTGGCCA GCCTGGCCAT 
GGTGCCCGAG CAGAGGGCGA GCCCAGCAGC AGGGGCCCAC AAGAGCCCCG GCCACACTCA 
GGGGGCGGCT CCCAGTCCCC CGCCTCCCTG GGGCCCTCGA AGGGGAAGGG TGTCAGCTCG 
ACGTTCGAGA GCTTCAGGAT CAATATTCCT GGGAACACCG CGCATTCCGG GAGACTCTCC 
AACCCTGGCT TTTGTAACAC ATTCCGGCCT GTCGACAACA AGGTGCAGAG GAAAGAGAAC 
CCCTCGCCCC TCTTCTCCAT CAGGAAGACC AAACAGCTCA AGAGTGAGAT CTACGACCCT 
TTTGACCCTA CAGGCTCCGA CTCCAGTTCT GCCGGCAGCA GCCCCGAGCG CCTGGGCTCT 
GGTCTCCTAC CCTCCGAGAT CACTCGCACC ATCTCCATCA ACAGCCCAAA GGCCCCAACA 
TTGCAGACTG TGCGCTGTGT AACCTCCTAC ACAGTGGAGA CAGTCTTCGG GGCCGAGCCC 
GAGCCCCCGA GGAGGCCTTC TTCCGGTGTG CTCGAGCTCC GGGGCGAGGG GGCTCCCGAG 
GAGGCCTCCG ACCTGAAGCG AGAGGGGCTG GGCCAGGGGG CTGCTGAGGG CCGGGGCTCG 
GCCTCCCGGG CGCAGAGGCC GTCCCCATCC GAGCCGTGGG AGGACAAGGA CCAGCTACCC 
TGCAGTACTT TCTTTGGCTC CGAGGAGCGG ACAGTGACCT GCGTGACTGT GGCAGAGCCA 
GAGGCCCCAC CCAGCCCGGA CGCGCCACAG ACAACCACCC ACAGAATCGT GGAGCTCAGG 
TCCCCGTCTT GCTCCCGCTC CACGTCCAGC TCCCGTGGCA GGAAGAAAGC CAAGAGGAAG 
CGGGCCTCTG CCAGGGAGCA CCGGAGGACC CGCTCGGGCT CCCACTCGGG CTCCCGCTCT 
GGGGACAGGA GCTCACGGTC CGTGTCTCCA CCGGTGGGGG AGGACCACCC CAAGAGGCAG 
CAGACCAAGT CCCGGGGCCG GAGGTCTTCT AGTGACCACT CCAGCAGCCA CGAGCGAGCT 
AAGCGGAAGA AGGCGAAGGA GGAGGGCAGG AGGAGGAAGA GGGACTCCTG GGGTCACGGC 
CGGCGGAGGT CCAGGTCCCG CTCAGGCAGC CCGGGGAGCT CGTCCCATGA ACGCAGGGAG 
AGCAGGAGAA GGAAGCGGAG GCGGTCGGGG TCCAGGTCTC GGGGGAGGGA GTGCTCACCC 
GCCAGCAGCC TGGACAGAGC CCGGAGACAC CGGCACCCGA GGGAGAGGAG CCGGGAGCGG 
CCCCGCATCA GGTGGGGCTC CCACGAGAGG AGGAAGCGGA GGTCCAGGTC ACCGAGGTCG 
CCAAGCACGG AGCACAGGTC CCGGGAGCAC CAGCGGCCTC GTTCCCATGA GAAGCGGCCA 
AGGCCTCGTT CCCCGGAGAG GAAGTTGGCA GCTGCTCCCC AGGAGGAGCA GAAGCCTGAC 
AGGGAGCAGC CAGCCAGGCC ACCGGCCTCA GTGGGAGCAG ATGCCTCACC GGCGGGGGCT 
GAGACTCACA AGGTGGCTGT GGAGGTCCCA GCTGAGTGTC CGCCCGAGGA CCTTGATTAC 
GGTGACTCTG TGGAGGCGGG GCACATCTTT GAGGATTTTT CAAGCGAAGT CTTCCTCATG 
CAGCTTGATG ACATGAGCTC CCCGCCCTCC CCGGAGAGCA CGGACTCCTC CCCAGAGCGA 
GACTTCCCAC CCAACCCTGC CGTGCCGCCC GCCAGCCAGC AGCAGGACGC CAGCCTGGCC 
GTGGCTGTCA TCCGACGGGA GGTGTCCCTG ATCCATGGCG AAGATGCCGC ACAGCCCCCG 
CCCCAGGCGC AGGGCCCCGA CGAGAAGCCC CTGCTCCAGC AGGATGCAGC TGGGGCCGGC 
TCAGTGCCCA GCGCCCTGGG GAGCCAGGCT GTGGGCGGGG TGCCCGTGGG AAAGGAGGAA 
GGCCCCTCGC AGACCCCCTT GCTGCGGGCC AAAGCCCTGG TGAAGAGAGT CACCTGGAAC 
CTCCAGGACG CGGAGAGCAG CGCCCCTGCC GAGGACCGAG GCCTGCGGAC GCCGCTTCAC 
AGGCCTCAGA AGCCCCGGGA AGGGGTCTGG GAAGCAGAGG ACATGGGCCC CTCGGTGGGC 
TTCCAGCAGA CATCCTTCTC TGAGCCCCCT CCCCCCGGCT ATGCGCTTCC AGAGTCTGGC 
TTCCCTGACG CTGAGCCCTC TCAGGTTTAT ACGTCCAACC TGCCTCCCGC CCCAGCTCTG 
CCCGTGAGCC TCCCACCCTA CGCACCGGCC AGCCAGCCCA CAGTGCAGTT CATCCTGCAG 
GGGAGCCTGC CCCTGGCCAG CTGTGGGGTT GCACAGAGCC CGGCCCCCGT GCCCACCTCC 
CTGGCCACGG CCTCGGAGCC AGCCGGCCAC TCCACTGGTG CCAGCAACTC CGAGGAGAGG 
ACGGCCGCTC CCAGGCCTGC CGCAGAGAAG GCCAAGAATG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGGGCAGT GGAGGAAGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CGAAGGACGA GTACAAGGAC ATCCTCCGCA AGGCGGTACA GAAGATATGC 
CACAGCAAGA GCGGGGAGAT CAACCCCGTG AAGGTGGGCA ACCTGGTCAA GGCCTATGTG 
GACAAATACA GACACATGCG CAGGCACCGC AGGGCCGAGG CTGGCGAGGA GGCGCCCGCC 
CAGGGCCCTG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976684.1
CDS	240..5117
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_014121209.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121209.1

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGT 
GAGGACCAGT CTACAAACCC TGCTTCCCCT TTGAGCGCCA AGAGGAGGGT TCTGTCCCAG 
TCAGCACTGC GTTCTCACCG GCCCGTGGCC AGGCCTGTGG CCATGGGGCT CTCCAGGAGG 
AGTGTTCCTG CTGCGGCGCC TGAGCCAGAA GTGGATGAGG CCCCGGTCCC TGACCTGGTG 
GGGAGTATCC TTTCGGGCCA GAGCCTCCTC ATGATGAACG GCGCCGACAT CATCATCCAT 
CGGGACGGCT CTCTCAGCGC CAAGAGAGCA GCACCTGTTT CGTTCCCGCG GAGCGCAGGC 
AAGAGCACAG GCGGCTCATC GCAAGAGGGC AAGCACCCTG GGCCCAGGGC CTGCCTGCAG 
CCTAGAGCCC CGTGCGCGGG AAGCGGGTTG CAGAGCATGG GGCTGAGCTG TCGCAGTGGG 
CCCGCACCTT CCCGTGTCCC TGTGCTCTCA ACAGGGGCGA CCGTGAAACT GGACTCGTCG 
GTAACCCCTC CGTCAAGTCA GGCTCAGAAC CCATCAGGCG TGCGTGGGCC TGGCCTAAAG 
CAGAGGGACG ACCCCCGATG TGATGGCGAC AGGCCCACTC TGCCCGCCAG CGCCACACCC 
TCCAAGATCT CTGGTAGCTG CTCTCACTTG CCGCCGGCTG GTGCGCTGCT GGGCCAGGCC 
CTGAAGCCAG CTCCCAGGAG GCCTGACATA TCTGAGCTAC CCAGAATACC AAAGGTCAGA 
AGGGAGGACT GTGGCGGCAG ACGGTCGGAT GCGGCCCCCA CCAGTGAGCA GCGCGTCGAG 
ATCCCCAGCT CCTGCATCAG CCGCCTCACG GGCAGGGAGG GCCCTGGCCA GCCTGGCCAT 
GGTGCCCGAG CAGAGGGCGA GCCCAGCAGC AGGGGCCCAC AAGAGCCCCG GCCACACTCA 
GGGGGCGGCT CCCAGTCCCC CGCCTCCCTG GGGCCCTCGA AGGGGAAGGG TGTCAGCTCG 
ACGTTCGAGA GCTTCAGGAT CAATATTCCT GGGAACACCG CGCATTCCGG GAGACTCTCC 
AACCCTGGCT TTTGTAACAC ATTCCGGCCT GTCGACAACA AGGTGCAGAG GAAAGAGAAC 
CCCTCGCCCC TCTTCTCCAT CAGGAAGACC AAACAGCTCA AGAGTGAGAT CTACGACCCT 
TTTGACCCTA CAGGCTCCGA CTCCAGTTCT GCCGGCAGCA GCCCCGAGCG CCTGGGCTCT 
GGTCTCCTAC CCTCCGAGAT CACTCGCACC ATCTCCATCA ACAGCCCAAA GGCCCCAACA 
TTGCAGACTG TGCGCTGTGT AACCTCCTAC ACAGTGGAGA CAGTCTTCGG GGCCGAGCCC 
GAGCCCCCGA GGAGGCCTTC TTCCGGTGTG CTCGAGCTCC GGGGCGAGGG GGCTCCCGAG 
GAGGCCTCCG ACCTGAAGCG AGAGGGGCTG GGCCAGGGGG CTGCTGAGGG CCGGGGCTCG 
GCCTCCCGGG CGCAGAGGCC GTCCCCATCC GAGCCGTGGG AGGACAAGGA CCAGCTACCC 
TGCAGTACTT TCTTTGGCTC CGAGGAGCGG ACAGTGACCT GCGTGACTGT GGCAGAGCCA 
GAGGCCCCAC CCAGCCCGGA CGCGCCACAG ACAACCACCC ACAGAATCGT GGAGCTCAGG 
TCCCCGTCTT GCTCCCGCTC CACGTCCAGC TCCCGTGGCA GGAAGAAAGC CAAGAGGAAG 
CGGGCCTCTG CCAGGGAGCA CCGGAGGACC CGCTCGGGCT CCCACTCGGG CTCCCGCTCT 
GGGGACAGGA GCTCACGGTC CGTGTCTCCA CCGGTGGGGG AGGACCACCC CAAGAGGCAG 
CAGACCAAGT CCCGGGGCCG GAGGTCTTCT AGTGACCACT CCAGCAGCCA CGAGCGAGCT 
AAGCGGAAGA AGGCGAAGGA GGAGGGCAGG AGGAGGAAGA GGGACTCCTG GGGTCACGGC 
CGGCGGAGGT CCAGGTCCCG CTCAGGCAGC CCGGGGAGCT CGTCCCATGA ACGCAGGGAG 
AGCAGGAGAA GGAAGCGGAG GCGGTCGGGG TCCAGGTCTC GGGGGAGGGA GTGCTCACCC 
GCCAGCAGCC TGGACAGAGC CCGGAGACAC CGGCACCCGA GGGAGAGGAG CCGGGAGCGG 
CCCCGCATCA GGTGGGGCTC CCACGAGAGG AGGAAGCGGA GGTCCAGGTC ACCGAGGTCG 
CCAAGCACGG AGCACAGGTC CCGGGAGCAC CAGCGGCCTC GTTCCCATGA GAAGCGGCCA 
AGGCCTCGTT CCCCGGAGAG GAAGTTGGCA GCTGCTCCCC AGGAGGAGCA GAAGCCTGAC 
AGGGAGCAGC CAGCCAGGCC ACCGGCCTCA GTGGGAGCAG ATGCCTCACC GGCGGGGGCT 
GAGACTCACA AGGTGGCTGT GGAGGTCCCA GCTGAGTGTC CGCCCGAGGA CCTTGATTAC 
GGTGACTCTG TGGAGGCGGG GCACATCTTT GAGGATTTTT CAAGCGAAGT CTTCCTCATG 
CAGCTTGATG ACATGAGCTC CCCGCCCTCC CCGGAGAGCA CGGACTCCTC CCCAGAGCGA 
GACTTCCCAC CCAACCCTGC CGTGCCGCCC GCCAGCCAGC AGCAGGACGC CAGCCTGGCC 
GTGGCTGTCA TCCGACGGGA GGTGTCCCTG ATCCATGGCG AAGATGCCGC ACAGCCCCCG 
CCCCAGGCGC AGGGCCCCGA CGAGAAGCCC CTGCTCCAGC AGGATGCAGC TGGGGCCGGC 
TCAGTGCCCA GCGCCCTGGG GAGCCAGGCT GTGGGCGGGG TGCCCGTGGG AAAGGAGGAA 
GGCCCCTCGC AGACCCCCTT GCTGCGGGCC AAAGCCCTGG TGAAGAGAGT CACCTGGAAC 
CTCCAGGACG CGGAGAGCAG CGCCCCTGCC GAGGACCGAG GCCTGCGGAC GCCGCTTCAC 
AGGCCTCAGA AGCCCCGGGA AGGGGTCTGG GAAGCAGAGG ACATGGGCCC CTCGGTGGGC 
TTCCAGCAGA CATCCTTCTC TGAGCCCCCT CCCCCCGGCT ATGCGCTTCC AGAGTCTGGC 
TTCCCTGACG CTGAGCCCTC TCAGGTTTAT ACGTCCAACC TGCCTCCCGC CCCAGCTCTG 
CCCGTGAGCC TCCCACCCTA CGCACCGGCC AGCCAGCCCA CAGTGCAGTT CATCCTGCAG 
GGGAGCCTGC CCCTGGCCAG CTGTGGGGTT GCACAGAGCC CGGCCCCCGT GCCCACCTCC 
CTGGCCACGG CCTCGGAGCC AGCCGGCCAC TCCACTGGTG CCAGCAACTC CGAGGAGAGG 
ACGGCCGCTC CCAGGCCTGC CGCAGAGAAG GCCAAGAATG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGGGCAGT GGAGGAAGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CGAAGGACGA GTACAAGGAC ATCCTCCGCA AGGCGGTACA GAAGATATGC 
CACAGCAAGA GCGGGGAGAT CAACCCCGTG AAGGTGGGCA ACCTGGTCAA GGCCTATGTG 
GACAAATACA GACACATGCG CAGGCACCGC AGGGCCGAGG CTGGCGAGGA GGCGCCCGCC 
CAGGGCCCTG AGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_014121208.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976683.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OCe19847
Clone ID Related Accession (Same CDS sequence)	XM_014121208.1 , XM_005631089.2 , XM_014121208.2 , XM_022405287.1 , XM_005631089.3
Accession Version	XM_014121208.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976683.1
CDS	122..5002
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_014121208.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121208.1

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005631089.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631146.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

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CloneID	OCe19847
Clone ID Related Accession (Same CDS sequence)	XM_014121208.1 , XM_005631089.2 , XM_014121208.2 , XM_022405287.1 , XM_005631089.3
Accession Version	XM_005631089.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631146.1
CDS	240..5120
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_005631089.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631089.2

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_014121208.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976683.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OCe19847
Clone ID Related Accession (Same CDS sequence)	XM_014121208.1 , XM_005631089.2 , XM_014121208.2 , XM_022405287.1 , XM_005631089.3
Accession Version	XM_014121208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014121208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976683.1
CDS	115..4995
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_014121208.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014121208.2

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_022405287.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260995.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

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CloneID	OCe19847
Clone ID Related Accession (Same CDS sequence)	XM_014121208.1 , XM_005631089.2 , XM_014121208.2 , XM_022405287.1 , XM_005631089.3
Accession Version	XM_022405287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260995.1
CDS	47..4927
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_022405287.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405287.1

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005631089.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005631146.1 Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

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CloneID	OCe19847
Clone ID Related Accession (Same CDS sequence)	XM_014121208.1 , XM_005631089.2 , XM_014121208.2 , XM_022405287.1 , XM_005631089.3
Accession Version	XM_005631089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631146.1
CDS	224..5104
Translation	MDDDSLDELVDRSPGPDGHRRLSPAALASNAEESSDGTSEGSEDDTGSERSYSTDGEDQSGSEDSEEGGRGEEDMEALVAVVDTQGKLEDNGAFNSDDDAESCPICLNTFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTIFKCICIRARFGGKILKKIPVENARAGEEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGAAADADAGPVSEEEVALLLADVVPTTSRLRPHAGRTRAIARTRQSERVRATVNRNRISTARRIQHVPRYLMSSLLDETIEAVAAGLSTAVYQRPVTPRAPKRRRRTGRRKKASGRRKAQPRSSVKSRSSGARSKKRQGRVKKRKGNKSKNEATARSRIARTLGLRGPACGGCIPSVHKPAEPSLGLMRADIGAASLSLFGDPYELDPFDSSEDQSTNPASPLSAKRRVLSQSALRSHRPVARPVAMGLSRRSVPAAAPEPEVDEAPVPDLVGSILSGQSLLMMNGADIIIHRDGSLSAKRAAPVSFPRSAGKSTGGSSQEGKHPGPRACLQPRAPCAGSGLQSMGLSCRSGPAPSRVPVLSTGATVKLDSSVTPPSSQAQNPSGVRGPGLKQRDDPRCDGDRPTLPASATPSKISGSCSHLPPAGALLGQALKPAPRRPDISELPRIPKVRREDCGGRRSDAAPTSEQRVEIPSSCISRLTGREGPGQPGHGARAEGEPSSRGPQEPRPHSGGGSQSPASLGPSKGKGVSSTFESFRINIPGNTAHSGRLSNPGFCNTFRPVDNKVQRKENPSPLFSIRKTKQLKSEIYDPFDPTGSDSSSAGSSPERLGSGLLPSEITRTISINSPKAPTLQTVRCVTSYTVETVFGAEPEPPRRPSSGVLELRGEGAPEEASDLKREGLGQGAAEGRGSASRAQRPSPSEPWEDKDQLPCSTFFGSEERTVTCVTVAEPEAPPSPDAPQTTTHRIVELRSPSCSRSTSSSRGRKKAKRKRASAREHRRTRSGSHSGSRSGDRSSRSVSPPVGEDHPKRQQTKSRGRRSSSDHSSSHERAKRKKAKEEGRRRKRDSWGHGRRRSRSRSGSPGSSSHERRESRRRKRRRSGSRSRGRECSPASSLDRARRHRHPRERSRERPRIRWGSHERRKRRSRSPRSPSTEHRSREHQRPRSHEKRPRPRSPERKLAAAPQEEQKPDREQPARPPASVGADASPAGAETHKVAVEVPAECPPEDLDYGDSVEAGHIFEDFSSEVFLMQLDDMSSPPSPESTDSSPERDFPPNPAVPPASQQQDASLAVAVIRREVSLIHGEDAAQPPPQAQGPDEKPLLQQDAAGAGSVPSALGSQAVGGVPVGKEEGPSQTPLLRAKALVKRVTWNLQDAESSAPAEDRGLRTPLHRPQKPREGVWEAEDMGPSVGFQQTSFSEPPPPGYALPESGFPDAEPSQVYTSNLPPAPALPVSLPPYAPASQPTVQFILQGSLPLASCGVAQSPAPVPTSLATASEPAGHSTGASNSEERTAAPRPAAEKAKNEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKDEYKDILRKAVQKICHSKSGEINPVKVGNLVKAYVDKYRHMRRHRRAEAGEEAPAQGPES

Target ORF information:

RefSeq Version	XM_005631089.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631089.3

ORF Insert Sequence:

ATGGATGACG ACAGCTTGGA TGAGCTTGTG GACCGAAGCC CAGGGCCAGA TGGACACCGT 
CGACTCAGCC CTGCTGCCCT GGCCAGCAAT GCTGAAGAAA GCAGTGATGG CACCAGCGAG 
GGCTCTGAGG ACGACACGGG CAGTGAGCGC AGCTACAGCA CTGATGGAGA GGACCAGTCT 
GGGTCCGAAG ATTCTGAAGA AGGAGGAAGA GGAGAAGAGG ACATGGAGGC GTTAGTAGCT 
GTGGTGGACA CTCAGGGGAA GCTGGAAGAC AACGGTGCAT TTAATTCTGA TGATGATGCC 
GAGAGCTGCC CAATTTGTCT CAATACTTTC CGGGACCAGG CTGTGGGGAC ACCCGAGAAC 
TGTGCCCATT ATTTCTGTCT GGACTGTATC GTGGAATGGT CCAAGAATGC CAATTCCTGT 
CCCGTCGATC GAACTATATT TAAATGTATT TGTATTCGAG CCCGGTTTGG TGGTAAAATC 
CTGAAGAAGA TTCCAGTGGA GAATGCCAGA GCCGGAGAAG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCCG GAGTGACCGG GAGGACCGTC TGCTCCTCTG TGACGGCTGC 
GACGCCGGGT ACCACATGGA GTGTTTAGAC CCTCCTCTCC AGGAGGTACC GGTGGATGAG 
TGGTTCTGTC CAGAATGTGC TGCGCCTGGA GCTGCTGCCG ACGCCGATGC AGGTCCTGTG 
AGCGAGGAAG AGGTGGCCCT GCTCTTGGCT GACGTGGTGC CCACCACCAG CCGGCTTCGG 
CCGCACGCAG GGAGGACCCG AGCTATTGCC AGGACGAGGC AGAGCGAGAG GGTCCGCGCG 
ACCGTGAACC GGAATCGGAT CTCCACCGCC AGGAGGATCC AGCATGTCCC GCGGTACCTC 
ATGTCTTCTC TGCTGGATGA GACCATCGAG GCGGTCGCTG CGGGTCTGAG CACTGCCGTG 
TACCAGCGCC CCGTGACGCC CCGGGCCCCC AAGCGCAGGA GGAGAACAGG AAGACGGAAG 
AAAGCATCAG GAAGAAGGAA AGCGCAGCCC AGGTCATCTG TGAAGAGCAG GAGCTCTGGG 
GCGAGGTCTA AGAAACGCCA GGGCCGCGTG AAGAAGAGAA AGGGGAACAA GTCAAAGAAT 
GAAGCCACAG CTCGCTCCCG TATCGCACGG ACACTGGGCC TCCGGGGTCC GGCCTGCGGA 
GGCTGCATCC CCTCTGTGCA CAAGCCGGCG GAGCCCTCTC TGGGGCTGAT GCGTGCGGAC 
ATCGGAGCGG CCTCTCTGTC GCTCTTTGGA GACCCTTATG AGCTGGACCC CTTTGACAGC 
AGTGAGGACC AGTCTACAAA CCCTGCTTCC CCTTTGAGCG CCAAGAGGAG GGTTCTGTCC 
CAGTCAGCAC TGCGTTCTCA CCGGCCCGTG GCCAGGCCTG TGGCCATGGG GCTCTCCAGG 
AGGAGTGTTC CTGCTGCGGC GCCTGAGCCA GAAGTGGATG AGGCCCCGGT CCCTGACCTG 
GTGGGGAGTA TCCTTTCGGG CCAGAGCCTC CTCATGATGA ACGGCGCCGA CATCATCATC 
CATCGGGACG GCTCTCTCAG CGCCAAGAGA GCAGCACCTG TTTCGTTCCC GCGGAGCGCA 
GGCAAGAGCA CAGGCGGCTC ATCGCAAGAG GGCAAGCACC CTGGGCCCAG GGCCTGCCTG 
CAGCCTAGAG CCCCGTGCGC GGGAAGCGGG TTGCAGAGCA TGGGGCTGAG CTGTCGCAGT 
GGGCCCGCAC CTTCCCGTGT CCCTGTGCTC TCAACAGGGG CGACCGTGAA ACTGGACTCG 
TCGGTAACCC CTCCGTCAAG TCAGGCTCAG AACCCATCAG GCGTGCGTGG GCCTGGCCTA 
AAGCAGAGGG ACGACCCCCG ATGTGATGGC GACAGGCCCA CTCTGCCCGC CAGCGCCACA 
CCCTCCAAGA TCTCTGGTAG CTGCTCTCAC TTGCCGCCGG CTGGTGCGCT GCTGGGCCAG 
GCCCTGAAGC CAGCTCCCAG GAGGCCTGAC ATATCTGAGC TACCCAGAAT ACCAAAGGTC 
AGAAGGGAGG ACTGTGGCGG CAGACGGTCG GATGCGGCCC CCACCAGTGA GCAGCGCGTC 
GAGATCCCCA GCTCCTGCAT CAGCCGCCTC ACGGGCAGGG AGGGCCCTGG CCAGCCTGGC 
CATGGTGCCC GAGCAGAGGG CGAGCCCAGC AGCAGGGGCC CACAAGAGCC CCGGCCACAC 
TCAGGGGGCG GCTCCCAGTC CCCCGCCTCC CTGGGGCCCT CGAAGGGGAA GGGTGTCAGC 
TCGACGTTCG AGAGCTTCAG GATCAATATT CCTGGGAACA CCGCGCATTC CGGGAGACTC 
TCCAACCCTG GCTTTTGTAA CACATTCCGG CCTGTCGACA ACAAGGTGCA GAGGAAAGAG 
AACCCCTCGC CCCTCTTCTC CATCAGGAAG ACCAAACAGC TCAAGAGTGA GATCTACGAC 
CCTTTTGACC CTACAGGCTC CGACTCCAGT TCTGCCGGCA GCAGCCCCGA GCGCCTGGGC 
TCTGGTCTCC TACCCTCCGA GATCACTCGC ACCATCTCCA TCAACAGCCC AAAGGCCCCA 
ACATTGCAGA CTGTGCGCTG TGTAACCTCC TACACAGTGG AGACAGTCTT CGGGGCCGAG 
CCCGAGCCCC CGAGGAGGCC TTCTTCCGGT GTGCTCGAGC TCCGGGGCGA GGGGGCTCCC 
GAGGAGGCCT CCGACCTGAA GCGAGAGGGG CTGGGCCAGG GGGCTGCTGA GGGCCGGGGC 
TCGGCCTCCC GGGCGCAGAG GCCGTCCCCA TCCGAGCCGT GGGAGGACAA GGACCAGCTA 
CCCTGCAGTA CTTTCTTTGG CTCCGAGGAG CGGACAGTGA CCTGCGTGAC TGTGGCAGAG 
CCAGAGGCCC CACCCAGCCC GGACGCGCCA CAGACAACCA CCCACAGAAT CGTGGAGCTC 
AGGTCCCCGT CTTGCTCCCG CTCCACGTCC AGCTCCCGTG GCAGGAAGAA AGCCAAGAGG 
AAGCGGGCCT CTGCCAGGGA GCACCGGAGG ACCCGCTCGG GCTCCCACTC GGGCTCCCGC 
TCTGGGGACA GGAGCTCACG GTCCGTGTCT CCACCGGTGG GGGAGGACCA CCCCAAGAGG 
CAGCAGACCA AGTCCCGGGG CCGGAGGTCT TCTAGTGACC ACTCCAGCAG CCACGAGCGA 
GCTAAGCGGA AGAAGGCGAA GGAGGAGGGC AGGAGGAGGA AGAGGGACTC CTGGGGTCAC 
GGCCGGCGGA GGTCCAGGTC CCGCTCAGGC AGCCCGGGGA GCTCGTCCCA TGAACGCAGG 
GAGAGCAGGA GAAGGAAGCG GAGGCGGTCG GGGTCCAGGT CTCGGGGGAG GGAGTGCTCA 
CCCGCCAGCA GCCTGGACAG AGCCCGGAGA CACCGGCACC CGAGGGAGAG GAGCCGGGAG 
CGGCCCCGCA TCAGGTGGGG CTCCCACGAG AGGAGGAAGC GGAGGTCCAG GTCACCGAGG 
TCGCCAAGCA CGGAGCACAG GTCCCGGGAG CACCAGCGGC CTCGTTCCCA TGAGAAGCGG 
CCAAGGCCTC GTTCCCCGGA GAGGAAGTTG GCAGCTGCTC CCCAGGAGGA GCAGAAGCCT 
GACAGGGAGC AGCCAGCCAG GCCACCGGCC TCAGTGGGAG CAGATGCCTC ACCGGCGGGG 
GCTGAGACTC ACAAGGTGGC TGTGGAGGTC CCAGCTGAGT GTCCGCCCGA GGACCTTGAT 
TACGGTGACT CTGTGGAGGC GGGGCACATC TTTGAGGATT TTTCAAGCGA AGTCTTCCTC 
ATGCAGCTTG ATGACATGAG CTCCCCGCCC TCCCCGGAGA GCACGGACTC CTCCCCAGAG 
CGAGACTTCC CACCCAACCC TGCCGTGCCG CCCGCCAGCC AGCAGCAGGA CGCCAGCCTG 
GCCGTGGCTG TCATCCGACG GGAGGTGTCC CTGATCCATG GCGAAGATGC CGCACAGCCC 
CCGCCCCAGG CGCAGGGCCC CGACGAGAAG CCCCTGCTCC AGCAGGATGC AGCTGGGGCC 
GGCTCAGTGC CCAGCGCCCT GGGGAGCCAG GCTGTGGGCG GGGTGCCCGT GGGAAAGGAG 
GAAGGCCCCT CGCAGACCCC CTTGCTGCGG GCCAAAGCCC TGGTGAAGAG AGTCACCTGG 
AACCTCCAGG ACGCGGAGAG CAGCGCCCCT GCCGAGGACC GAGGCCTGCG GACGCCGCTT 
CACAGGCCTC AGAAGCCCCG GGAAGGGGTC TGGGAAGCAG AGGACATGGG CCCCTCGGTG 
GGCTTCCAGC AGACATCCTT CTCTGAGCCC CCTCCCCCCG GCTATGCGCT TCCAGAGTCT 
GGCTTCCCTG ACGCTGAGCC CTCTCAGGTT TATACGTCCA ACCTGCCTCC CGCCCCAGCT 
CTGCCCGTGA GCCTCCCACC CTACGCACCG GCCAGCCAGC CCACAGTGCA GTTCATCCTG 
CAGGGGAGCC TGCCCCTGGC CAGCTGTGGG GTTGCACAGA GCCCGGCCCC CGTGCCCACC 
TCCCTGGCCA CGGCCTCGGA GCCAGCCGGC CACTCCACTG GTGCCAGCAA CTCCGAGGAG 
AGGACGGCCG CTCCCAGGCC TGCCGCAGAG AAGGCCAAGA ATGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGGGC AGTGGAGGAA GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACGAAGGA CGAGTACAAG GACATCCTCC GCAAGGCGGT ACAGAAGATA 
TGCCACAGCA AGAGCGGGGA GATCAACCCC GTGAAGGTGG GCAACCTGGT CAAGGCCTAT 
GTGGACAAAT ACAGACACAT GCGCAGGCAC CGCAGGGCCG AGGCTGGCGA GGAGGCGCCC 
GCCCAGGGCC CTGAGAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PHRF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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