PHRF1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011520236.3	XP_011518538.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_011520236.2	XP_011518538.1	PHD and RING finger domain-containing protein 1 isoform X2
NM_020901.3	NP_065952.2	PHD and RING finger domain-containing protein 1 isoform 2
XM_011520237.3	XP_011518539.1	PHD and RING finger domain-containing protein 1 isoform X4
NM_001286581.1	NP_001273510.1	PHD and RING finger domain-containing protein 1 isoform 1
NM_001286582.2	NP_001273511.1	PHD and RING finger domain-containing protein 1 isoform 3
NM_001286583.2	NP_001273512.1	PHD and RING finger domain-containing protein 1 isoform 4
NM_001286581.2	NP_001273510.1	PHD and RING finger domain-containing protein 1 isoform 1
XM_005253025.5	XP_005253082.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_011520237.2	XP_011518539.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_005253025.4	XP_005253082.1	PHD and RING finger domain-containing protein 1 isoform X1
NM_001286583.1	NP_001273512.1	PHD and RING finger domain-containing protein 1 isoform 4
NM_001286582.1	NP_001273511.1	PHD and RING finger domain-containing protein 1 isoform 3
NM_020901.4	NP_065952.2	PHD and RING finger domain-containing protein 1 isoform 2
XM_005253027.3	XP_005253084.1	PHD and RING finger domain-containing protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28169	NM_020901.4 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28171	NM_001286583.2 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28192	NM_001286581.2 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28216	NM_001286582.2 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu44926	XM_005253025.5 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu44927	XM_005253027.3 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70810	XM_011520236.3 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70811	XM_011520237.3 Latest version!	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OHu70810	XM_011520236.2	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28192	NM_001286581.1	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu70811	XM_011520237.2	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu44926	XM_005253025.4	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28171	NM_001286583.1	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28216	NM_001286582.1	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu28169	NM_020901.3	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

PHRF1 ( NM_020901.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065952.2 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28169
Clone ID Related Accession (Same CDS sequence)	NM_020901.3 , NM_020901.4
Accession Version	NM_020901.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144293.1 and BC146771.1. On Nov 8, 2013 this sequence version replaced NM_020901.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065952.2
CDS	169..5115
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_020901.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020901.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_020901.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065952.2 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.

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CloneID	OHu28169
Clone ID Related Accession (Same CDS sequence)	NM_020901.3 , NM_020901.4
Accession Version	NM_020901.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144293.1 and BC146771.1. On May 31, 2019 this sequence version replaced NM_020901.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144293.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065952.2
CDS	145..5091
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_020901.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020901.4

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286583.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273512.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu28171
Clone ID Related Accession (Same CDS sequence)	NM_001286583.2 , NM_001286583.1
Accession Version	NM_001286583.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144294.1, AP006284.2, BC144293.1 and BC146771.1. On May 31, 2019 this sequence version replaced NM_001286583.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAAGC AGCGTGGGCA GCAGTGGGGA CTCTGGGGAC 
GACAGTGACA GCGAGCATGG AGATGGCACA GACGGAGAAG ACGAGGGGGC GTCTGAGGAG 
GAAGACCTGG AAGACAGATC TGGTTCCGAG GATTCTGAAG ACGACGGGGA GACATTGCTG 
GAGGTAGCGG GTACTCAGGG GAAACTGGAA GCCGCTGGCT CTTTCAATTC TGATGATGAT 
GCAGAGAGCT GCCCAATCTG TCTCAACGCA TTCAGAGACC AGGCCGTGGG GACGCCGGAG 
AACTGTGCCC ATTACTTCTG CCTGGACTGC ATTGTCGAAT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTCT ATTTAAGTGC ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTAAGAA AGATCCCAGT GGAGAACACC AAAGCGAGCG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCAG GAGCGACCGT GAGGACAGGC TTTTGCTCTG CGACGGCTGC 
GATGCGGGGT ACCACATGGA ATGCTTGGAC CCCCCTCTCC AGGAGGTGCC GGTGGACGAG 
TGGTTCTGCC CGGAATGTGC TGCGCCTGGT GTTGTCCTTG CCGCTGATGC GGGTCCCGTG 
AGTGAGGAGG AGGTCTCCCT GCTCTTGGCT GATGTGGTGC CCACCACCAG CAGGCTTCGG 
CCTCGAGCAG GTAGGACCCG GGCGATAGCC AGGACACGGC AGAGTGAGAG AGTGAGAGCA 
ACCGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGGTCC AGCACACACC AGGGCGCCTC 
GGGTCTTCCC TGCTGGATGA AGCCATCGAG GCTGTGGCGA CTGGCCTGAG CACTGCCGTG 
TATCAGCGCC CCCTGACGCC GCGCACTCCC GCCCGACGGA AGAGGAAGAC AAGAAGACGG 
AAGAAAGTGC CGGGAAGAAA GAAAACCCCG TCCGGACCAT CCGCAAAAAG TAAGAGCTCA 
GCGACAAGAT CTAAGAAACG CCAACATCGA GTGAAGAAGA GAAGAGGGAA GAAGGTAAAG 
AGTGAAGCCA CCACTCGCTC TCGAATCGCG CGGACGCTGG GCCTGCGCAG GCCTGTTCAC 
AGCAGCTGCA TCCCGTCAGT GTTGAAGCCA GTGGAGCCCT CTTTGGGGCT GCTGAGAGCG 
GATATTGGAG CTGCCTCTCT GTCTCTGTTT GGAGATCCTT ATGAGCTGGA TCCCTTCGAC 
AGCAGTGAAG AGCTTTCTGC AAACCCTCTT TCCCCTCTGA GTGCCAAGAG ACGGGCTCTG 
TCCCGGTCAG CCCTGCAGTC CCACCAGCCC GTGGCCAGGC CCGTCTCCGT GGGGCTTTCC 
AGGAGGCGCC TCCCTGCCGC GGTGCCAGAG CCAGACTTGG AGGAGGAGCC AGTGCCTGAC 
CTGCTGGGCA GCATCCTGTC GGGCCAGAGC CTCCTGATGC TGGGCAGCAG TGATGTCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCGGCTC CAGTTTCTTT TCAGCGAAAC 
TCAGGCAGTC TGTCCAGAGG GGAAGAAGGA TTCAAGGGCT GCCTGCAGCC CCGAGCACTG 
CCCTCCGGGA GCCCGGCCCA AGGCCCGTCA GGAAACAGGC CACAGAGCAC AGGGCTCAGC 
TGTCAAGGCA GGTCCCGCAC CCCCGCCCGC ACCGCGGGGG CGCCTGTGAG GCTGGACTTG 
CCAGCAGCCC CTGGGGCGGT TCAGGCTCGG AACTTGTCAA ATGGGAGTGT GCCTGGCTTC 
AGACAGAGCC ACAGCCCCTG GTTCAACGGC ACCAACAAGC ACACCTTGCC CCTTGCCTCT 
GCCGCGTCTA AGATCTCAAG CAGAGATTCT AAGCCCCCAT GTCGCAGTGT GGTGCCGGGG 
CCTCCCCTGA AGCCAGCGCC CAGAAGAACA GACATCTCTG AGCTACCCAG GATACCAAAG 
ATCAGGAGAG ATGACGGTGG TGGCAGACGG GATGCGGCCC CGGCCCACGG GCAGAGCATT 
GAGATCCCCA GTGCCTGCAT CAGCCGACTG ACTGGCAGGG AGGGCACCGG GCAGCCAGGG 
CGAGGCACAC GGGCAGAGAG CGAGGCCAGC AGCAGGGTGC CCCGGGAGCC CGGGGTGCAC 
ACGGGCAGCT CCCGGCCCCC AGCCCCCAGC TCCCATGGCA GTTTGGCCCC ACTGGGACCA 
TCAAGAGGGA AAGGGGTCGG GTCGACCTTT GAGAGCTTCC GGATCAATAT TCCTGGAAAC 
ATGGCACATT CCAGCCAGCT CTCCAGCCCT GGCTTCTGTA ACACGTTCCG GCCTGTGGAC 
GATAAGGAGC AGAGGAAGGA GAACCCCTCA CCCCTCTTCT CCATCAAGAA GACGAAGCAG 
CTGCGGAGCG AGGTCTACGA CCCATCCGAC CCCACCGGCT CCGACTCCAG CGCCCCTGGC 
AGCAGCCCCG AGAGGTCTGG CCCCGGCCTC CTGCCCTCTG AGATCACACG AACCATCTCC 
ATCAACAGCC CGAAGGCCCA GACGGTGCAG GCTGTGCGCT GCGTCACCTC CTACACGGTG 
GAGAGCATCT TTGGTACAGA GCCCGAACCC CCTCTCGGAC CGTCCTCCGC CATGTCCAAG 
CTCCGGGGTG CAGTGGCTGC CGAGGGGGCC TCTGACACGG AGCGAGAGGA GCCCACAGAG 
AGCCAGGGCC TGGCTGCCCG GCTGCGGAGG CCATCCCCCC CAGAGCCCTG GGATGAGGAG 
GATGGGGCGT CTTGCAGCAC CTTCTTTGGC TCTGAGGAGC GGACGGTGAC CTGTGTGACT 
GTCGTGGAGC CGGAAGCCCC ACCCAGCCCG GACGTGCTGC AGGCTGCCAC CCACAGAGTC 
GTGGAGCTCA GGCCCCCTTC CCGGTCCCGC TCCACATCCA GCTCCCGCAG CAGGAAGAAG 
GCCAAGAGGA AGAGGGTGTC CAGGGAGCAC GGACGGACGC GCTCTGGGAC GCGCTCTGAA 
TCCAGGGACA GGAGCTCGAG GTCAGCGTCA CCATCAGTGG GTGAGGAGCG CCCCAGGAGG 
CAGCGGTCCA AGGCCAAGAG CCGGCGGTCC TCCAGTGACC GCTCCAGCAG CCGAGAGCGA 
GCTAAGAGGA AGAAAGCCAA GGACAAGAGC AGGGAGCACA GGCGGGGCCC CTGGGGCCAC 
AGCCGGAGGA CGTCCCGGTC GCGGTCGGGG AGCCCTGGCA GCTCTTCCTA TGAGCACTAT 
GAGAGTAGGA AGAAGAAGAA AAGGAGATCA GCGTCCAGAC CTCGGGGAAG GGAGTGCTCC 
CCCACCAGCA GCCTGGAGAG GCTCTGCAGG CACAAGCATC AGCGGGAACG CAGCCACGAG 
CGGCCAGACA GGAAGGAGAG TGTGGCGTGG CCCCGAGACC GGAGGAAGCG GAGGTCCCGG 
TCCCCAAGCT CGGAGCACAG GGCACGGGAG CACAGGCGGC CTCGGTCCCG TGAGAAGTGG 
CCGCAGACCC GGTCCCATTC CCCAGAGAGG AAGGGGGCTG TGAGGGAGGC TTCCCCAGCG 
CCCCTTGCAC AGGGGGAGCC AGGGCGGGAA GACCTCCCCA CCAGGTTGCC AGCCTTGGGG 
GAAGCACATG TCTCGCCGGA GGTGGCTACG GCCGACAAGG CCCCCCTGCA GGCTCCCCCT 
GTCCTGGAGG TGGCAGCTGA GTGTGAGCCG GACGACCTGG ACCTGGATTA TGGCGACTCC 
GTGGAGGCCG GACACGTCTT TGATGATTTC TCAAGCGACG CCGTTTTCAT CCAGCTCGAT 
GACATGAGCT CGCCACCTTC TCCCGAAAGC ACAGACTCTT CCCCGGAGCG AGACTTCCCA 
CTGAAGCCTG CGTTGCCCCC AGCCAGCCTG GCCGTGGCCG CCATCCAGAG GGAGGTGTCA 
TTGATGCACG ATGAAGACCC TTCGCAGCCC CCACCCCTGC CAGAGGGCAC CCAGGAGCCA 
CATTTGCTCA GGCCGGACGC GGCTGAGAAG GCTGAGGCAC CCAGTTCCCC GGATGTGGCG 
CCTGCGGGGA AGGAAGACAG CCCCTCTGCG AGTGGGAGGG TACAGGAGGC AGCCCGGCCT 
GAGGAGGTGG TTTCGCAGAC CCCCCTGCTG CGGTCCAGAG CCCTGGTGAA GCGGGTCACC 
TGGAACCTGC AGGAGTCGGA GAGCAGCGCC CCCGCCGAGG ACAGAGCCCC CCGGGCACCA 
CTTCACAGGC CACAGAAGCC CCGAGAAGGA GCCTGGGACA TGGAGGATGT GGCCCCCACA 
GGGGTCAGGC AGGTGTTCTC CGAGCTGCCC TTTCCCAGTC ACGTGCTTCC GGAACCCGGG 
TTCCCAGACA CAGACCCCTC TCAGGTTTAC AGCCCCGGCC TGCCGCCTGC CCCGGCCCAG 
CCCTCAAGCA TCCCACCCTG CGCACTGGTC AGCCAGCCCA CGGTCCAGTT CATCCTTCAG 
GGGAGCCTGC CGCTAGTGGG CTGTGGGGCA GCACAGACCC TGGCCCCAGT GCCCGCTGCC 
CTGACCCCAG CCTCAGAGCC AGCCAGTCAA GCCACTGCAG CCAGCAACTC GGAGGAGAAG 
ACCCCGGCCC CCAGGCTAGC TGCGGAGAAA ACCAAGAAGG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGTGCTGT GGAGGAGGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CCAAGGAGGA GTACAAGGAC ATCCTGCGCA AGGCCGTGCA GAAGATCTGC 
CACAGCAAGA GTGGAGAGAT CAACCCCGTG AAGGTGGCCA ACCTGGTGAA GGCGTACGTG 
GACAAGTACA GGCACATGCG CAGGCACAAG AAACCAGAGG CCGGGGAGGA GCCGCCCACG 
CAGGGGGCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273512.1
CDS	145..5082
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286583.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286583.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAAGC AGCGTGGGCA GCAGTGGGGA CTCTGGGGAC 
GACAGTGACA GCGAGCATGG AGATGGCACA GACGGAGAAG ACGAGGGGGC GTCTGAGGAG 
GAAGACCTGG AAGACAGATC TGGTTCCGAG GATTCTGAAG ACGACGGGGA GACATTGCTG 
GAGGTAGCGG GTACTCAGGG GAAACTGGAA GCCGCTGGCT CTTTCAATTC TGATGATGAT 
GCAGAGAGCT GCCCAATCTG TCTCAACGCA TTCAGAGACC AGGCCGTGGG GACGCCGGAG 
AACTGTGCCC ATTACTTCTG CCTGGACTGC ATTGTCGAAT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTCT ATTTAAGTGC ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTAAGAA AGATCCCAGT GGAGAACACC AAAGCGAGCG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCAG GAGCGACCGT GAGGACAGGC TTTTGCTCTG CGACGGCTGC 
GATGCGGGGT ACCACATGGA ATGCTTGGAC CCCCCTCTCC AGGAGGTGCC GGTGGACGAG 
TGGTTCTGCC CGGAATGTGC TGCGCCTGGT GTTGTCCTTG CCGCTGATGC GGGTCCCGTG 
AGTGAGGAGG AGGTCTCCCT GCTCTTGGCT GATGTGGTGC CCACCACCAG CAGGCTTCGG 
CCTCGAGCAG GTAGGACCCG GGCGATAGCC AGGACACGGC AGAGTGAGAG AGTGAGAGCA 
ACCGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGGTCC AGCACACACC AGGGCGCCTC 
GGGTCTTCCC TGCTGGATGA AGCCATCGAG GCTGTGGCGA CTGGCCTGAG CACTGCCGTG 
TATCAGCGCC CCCTGACGCC GCGCACTCCC GCCCGACGGA AGAGGAAGAC AAGAAGACGG 
AAGAAAGTGC CGGGAAGAAA GAAAACCCCG TCCGGACCAT CCGCAAAAAG TAAGAGCTCA 
GCGACAAGAT CTAAGAAACG CCAACATCGA GTGAAGAAGA GAAGAGGGAA GAAGGTAAAG 
AGTGAAGCCA CCACTCGCTC TCGAATCGCG CGGACGCTGG GCCTGCGCAG GCCTGTTCAC 
AGCAGCTGCA TCCCGTCAGT GTTGAAGCCA GTGGAGCCCT CTTTGGGGCT GCTGAGAGCG 
GATATTGGAG CTGCCTCTCT GTCTCTGTTT GGAGATCCTT ATGAGCTGGA TCCCTTCGAC 
AGCAGTGAAG AGCTTTCTGC AAACCCTCTT TCCCCTCTGA GTGCCAAGAG ACGGGCTCTG 
TCCCGGTCAG CCCTGCAGTC CCACCAGCCC GTGGCCAGGC CCGTCTCCGT GGGGCTTTCC 
AGGAGGCGCC TCCCTGCCGC GGTGCCAGAG CCAGACTTGG AGGAGGAGCC AGTGCCTGAC 
CTGCTGGGCA GCATCCTGTC GGGCCAGAGC CTCCTGATGC TGGGCAGCAG TGATGTCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCGGCTC CAGTTTCTTT TCAGCGAAAC 
TCAGGCAGTC TGTCCAGAGG GGAAGAAGGA TTCAAGGGCT GCCTGCAGCC CCGAGCACTG 
CCCTCCGGGA GCCCGGCCCA AGGCCCGTCA GGAAACAGGC CACAGAGCAC AGGGCTCAGC 
TGTCAAGGCA GGTCCCGCAC CCCCGCCCGC ACCGCGGGGG CGCCTGTGAG GCTGGACTTG 
CCAGCAGCCC CTGGGGCGGT TCAGGCTCGG AACTTGTCAA ATGGGAGTGT GCCTGGCTTC 
AGACAGAGCC ACAGCCCCTG GTTCAACGGC ACCAACAAGC ACACCTTGCC CCTTGCCTCT 
GCCGCGTCTA AGATCTCAAG CAGAGATTCT AAGCCCCCAT GTCGCAGTGT GGTGCCGGGG 
CCTCCCCTGA AGCCAGCGCC CAGAAGAACA GACATCTCTG AGCTACCCAG GATACCAAAG 
ATCAGGAGAG ATGACGGTGG TGGCAGACGG GATGCGGCCC CGGCCCACGG GCAGAGCATT 
GAGATCCCCA GTGCCTGCAT CAGCCGACTG ACTGGCAGGG AGGGCACCGG GCAGCCAGGG 
CGAGGCACAC GGGCAGAGAG CGAGGCCAGC AGCAGGGTGC CCCGGGAGCC CGGGGTGCAC 
ACGGGCAGCT CCCGGCCCCC AGCCCCCAGC TCCCATGGCA GTTTGGCCCC ACTGGGACCA 
TCAAGAGGGA AAGGGGTCGG GTCGACCTTT GAGAGCTTCC GGATCAATAT TCCTGGAAAC 
ATGGCACATT CCAGCCAGCT CTCCAGCCCT GGCTTCTGTA ACACGTTCCG GCCTGTGGAC 
GATAAGGAGC AGAGGAAGGA GAACCCCTCA CCCCTCTTCT CCATCAAGAA GACGAAGCAG 
CTGCGGAGCG AGGTCTACGA CCCATCCGAC CCCACCGGCT CCGACTCCAG CGCCCCTGGC 
AGCAGCCCCG AGAGGTCTGG CCCCGGCCTC CTGCCCTCTG AGATCACACG AACCATCTCC 
ATCAACAGCC CGAAGGCCCA GACGGTGCAG GCTGTGCGCT GCGTCACCTC CTACACGGTG 
GAGAGCATCT TTGGTACAGA GCCCGAACCC CCTCTCGGAC CGTCCTCCGC CATGTCCAAG 
CTCCGGGGTG CAGTGGCTGC CGAGGGGGCC TCTGACACGG AGCGAGAGGA GCCCACAGAG 
AGCCAGGGCC TGGCTGCCCG GCTGCGGAGG CCATCCCCCC CAGAGCCCTG GGATGAGGAG 
GATGGGGCGT CTTGCAGCAC CTTCTTTGGC TCTGAGGAGC GGACGGTGAC CTGTGTGACT 
GTCGTGGAGC CGGAAGCCCC ACCCAGCCCG GACGTGCTGC AGGCTGCCAC CCACAGAGTC 
GTGGAGCTCA GGCCCCCTTC CCGGTCCCGC TCCACATCCA GCTCCCGCAG CAGGAAGAAG 
GCCAAGAGGA AGAGGGTGTC CAGGGAGCAC GGACGGACGC GCTCTGGGAC GCGCTCTGAA 
TCCAGGGACA GGAGCTCGAG GTCAGCGTCA CCATCAGTGG GTGAGGAGCG CCCCAGGAGG 
CAGCGGTCCA AGGCCAAGAG CCGGCGGTCC TCCAGTGACC GCTCCAGCAG CCGAGAGCGA 
GCTAAGAGGA AGAAAGCCAA GGACAAGAGC AGGGAGCACA GGCGGGGCCC CTGGGGCCAC 
AGCCGGAGGA CGTCCCGGTC GCGGTCGGGG AGCCCTGGCA GCTCTTCCTA TGAGCACTAT 
GAGAGTAGGA AGAAGAAGAA AAGGAGATCA GCGTCCAGAC CTCGGGGAAG GGAGTGCTCC 
CCCACCAGCA GCCTGGAGAG GCTCTGCAGG CACAAGCATC AGCGGGAACG CAGCCACGAG 
CGGCCAGACA GGAAGGAGAG TGTGGCGTGG CCCCGAGACC GGAGGAAGCG GAGGTCCCGG 
TCCCCAAGCT CGGAGCACAG GGCACGGGAG CACAGGCGGC CTCGGTCCCG TGAGAAGTGG 
CCGCAGACCC GGTCCCATTC CCCAGAGAGG AAGGGGGCTG TGAGGGAGGC TTCCCCAGCG 
CCCCTTGCAC AGGGGGAGCC AGGGCGGGAA GACCTCCCCA CCAGGTTGCC AGCCTTGGGG 
GAAGCACATG TCTCGCCGGA GGTGGCTACG GCCGACAAGG CCCCCCTGCA GGCTCCCCCT 
GTCCTGGAGG TGGCAGCTGA GTGTGAGCCG GACGACCTGG ACCTGGATTA TGGCGACTCC 
GTGGAGGCCG GACACGTCTT TGATGATTTC TCAAGCGACG CCGTTTTCAT CCAGCTCGAT 
GACATGAGCT CGCCACCTTC TCCCGAAAGC ACAGACTCTT CCCCGGAGCG AGACTTCCCA 
CTGAAGCCTG CGTTGCCCCC AGCCAGCCTG GCCGTGGCCG CCATCCAGAG GGAGGTGTCA 
TTGATGCACG ATGAAGACCC TTCGCAGCCC CCACCCCTGC CAGAGGGCAC CCAGGAGCCA 
CATTTGCTCA GGCCGGACGC GGCTGAGAAG GCTGAGGCAC CCAGTTCCCC GGATGTGGCG 
CCTGCGGGGA AGGAAGACAG CCCCTCTGCG AGTGGGAGGG TACAGGAGGC AGCCCGGCCT 
GAGGAGGTGG TTTCGCAGAC CCCCCTGCTG CGGTCCAGAG CCCTGGTGAA GCGGGTCACC 
TGGAACCTGC AGGAGTCGGA GAGCAGCGCC CCCGCCGAGG ACAGAGCCCC CCGGGCACCA 
CTTCACAGGC CACAGAAGCC CCGAGAAGGA GCCTGGGACA TGGAGGATGT GGCCCCCACA 
GGGGTCAGGC AGGTGTTCTC CGAGCTGCCC TTTCCCAGTC ACGTGCTTCC GGAACCCGGG 
TTCCCAGACA CAGACCCCTC TCAGGTTTAC AGCCCCGGCC TGCCGCCTGC CCCGGCCCAG 
CCCTCAAGCA TCCCACCCTG CGCACTGGTC AGCCAGCCCA CGGTCCAGTT CATCCTTCAG 
GGGAGCCTGC CGCTAGTGGG CTGTGGGGCA GCACAGACCC TGGCCCCAGT GCCCGCTGCC 
CTGACCCCAG CCTCAGAGCC AGCCAGTCAA GCCACTGCAG CCAGCAACTC GGAGGAGAAG 
ACCCCGGCCC CCAGGCTAGC TGCGGAGAAA ACCAAGAAGG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGTGCTGT GGAGGAGGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CCAAGGAGGA GTACAAGGAC ATCCTGCGCA AGGCCGTGCA GAAGATCTGC 
CACAGCAAGA GTGGAGAGAT CAACCCCGTG AAGGTGGCCA ACCTGGTGAA GGCGTACGTG 
GACAAGTACA GGCACATGCG CAGGCACAAG AAACCAGAGG CCGGGGAGGA GCCGCCCACG 
CAGGGGGCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286583.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273512.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.

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CloneID	OHu28171
Clone ID Related Accession (Same CDS sequence)	NM_001286583.2 , NM_001286583.1
Accession Version	NM_001286583.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144294.1, AP006284.2, BC144293.1 and BC146771.1. On Nov 8, 2013 this sequence version replaced XM_005253028.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (4) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAAGC AGCGTGGGCA GCAGTGGGGA CTCTGGGGAC 
GACAGTGACA GCGAGCATGG AGATGGCACA GACGGAGAAG ACGAGGGGGC GTCTGAGGAG 
GAAGACCTGG AAGACAGATC TGGTTCCGAG GATTCTGAAG ACGACGGGGA GACATTGCTG 
GAGGTAGCGG GTACTCAGGG GAAACTGGAA GCCGCTGGCT CTTTCAATTC TGATGATGAT 
GCAGAGAGCT GCCCAATCTG TCTCAACGCA TTCAGAGACC AGGCCGTGGG GACGCCGGAG 
AACTGTGCCC ATTACTTCTG CCTGGACTGC ATTGTCGAAT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTCT ATTTAAGTGC ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTAAGAA AGATCCCAGT GGAGAACACC AAAGCGAGCG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCAG GAGCGACCGT GAGGACAGGC TTTTGCTCTG CGACGGCTGC 
GATGCGGGGT ACCACATGGA ATGCTTGGAC CCCCCTCTCC AGGAGGTGCC GGTGGACGAG 
TGGTTCTGCC CGGAATGTGC TGCGCCTGGT GTTGTCCTTG CCGCTGATGC GGGTCCCGTG 
AGTGAGGAGG AGGTCTCCCT GCTCTTGGCT GATGTGGTGC CCACCACCAG CAGGCTTCGG 
CCTCGAGCAG GTAGGACCCG GGCGATAGCC AGGACACGGC AGAGTGAGAG AGTGAGAGCA 
ACCGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGGTCC AGCACACACC AGGGCGCCTC 
GGGTCTTCCC TGCTGGATGA AGCCATCGAG GCTGTGGCGA CTGGCCTGAG CACTGCCGTG 
TATCAGCGCC CCCTGACGCC GCGCACTCCC GCCCGACGGA AGAGGAAGAC AAGAAGACGG 
AAGAAAGTGC CGGGAAGAAA GAAAACCCCG TCCGGACCAT CCGCAAAAAG TAAGAGCTCA 
GCGACAAGAT CTAAGAAACG CCAACATCGA GTGAAGAAGA GAAGAGGGAA GAAGGTAAAG 
AGTGAAGCCA CCACTCGCTC TCGAATCGCG CGGACGCTGG GCCTGCGCAG GCCTGTTCAC 
AGCAGCTGCA TCCCGTCAGT GTTGAAGCCA GTGGAGCCCT CTTTGGGGCT GCTGAGAGCG 
GATATTGGAG CTGCCTCTCT GTCTCTGTTT GGAGATCCTT ATGAGCTGGA TCCCTTCGAC 
AGCAGTGAAG AGCTTTCTGC AAACCCTCTT TCCCCTCTGA GTGCCAAGAG ACGGGCTCTG 
TCCCGGTCAG CCCTGCAGTC CCACCAGCCC GTGGCCAGGC CCGTCTCCGT GGGGCTTTCC 
AGGAGGCGCC TCCCTGCCGC GGTGCCAGAG CCAGACTTGG AGGAGGAGCC AGTGCCTGAC 
CTGCTGGGCA GCATCCTGTC GGGCCAGAGC CTCCTGATGC TGGGCAGCAG TGATGTCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCGGCTC CAGTTTCTTT TCAGCGAAAC 
TCAGGCAGTC TGTCCAGAGG GGAAGAAGGA TTCAAGGGCT GCCTGCAGCC CCGAGCACTG 
CCCTCCGGGA GCCCGGCCCA AGGCCCGTCA GGAAACAGGC CACAGAGCAC AGGGCTCAGC 
TGTCAAGGCA GGTCCCGCAC CCCCGCCCGC ACCGCGGGGG CGCCTGTGAG GCTGGACTTG 
CCAGCAGCCC CTGGGGCGGT TCAGGCTCGG AACTTGTCAA ATGGGAGTGT GCCTGGCTTC 
AGACAGAGCC ACAGCCCCTG GTTCAACGGC ACCAACAAGC ACACCTTGCC CCTTGCCTCT 
GCCGCGTCTA AGATCTCAAG CAGAGATTCT AAGCCCCCAT GTCGCAGTGT GGTGCCGGGG 
CCTCCCCTGA AGCCAGCGCC CAGAAGAACA GACATCTCTG AGCTACCCAG GATACCAAAG 
ATCAGGAGAG ATGACGGTGG TGGCAGACGG GATGCGGCCC CGGCCCACGG GCAGAGCATT 
GAGATCCCCA GTGCCTGCAT CAGCCGACTG ACTGGCAGGG AGGGCACCGG GCAGCCAGGG 
CGAGGCACAC GGGCAGAGAG CGAGGCCAGC AGCAGGGTGC CCCGGGAGCC CGGGGTGCAC 
ACGGGCAGCT CCCGGCCCCC AGCCCCCAGC TCCCATGGCA GTTTGGCCCC ACTGGGACCA 
TCAAGAGGGA AAGGGGTCGG GTCGACCTTT GAGAGCTTCC GGATCAATAT TCCTGGAAAC 
ATGGCACATT CCAGCCAGCT CTCCAGCCCT GGCTTCTGTA ACACGTTCCG GCCTGTGGAC 
GATAAGGAGC AGAGGAAGGA GAACCCCTCA CCCCTCTTCT CCATCAAGAA GACGAAGCAG 
CTGCGGAGCG AGGTCTACGA CCCATCCGAC CCCACCGGCT CCGACTCCAG CGCCCCTGGC 
AGCAGCCCCG AGAGGTCTGG CCCCGGCCTC CTGCCCTCTG AGATCACACG AACCATCTCC 
ATCAACAGCC CGAAGGCCCA GACGGTGCAG GCTGTGCGCT GCGTCACCTC CTACACGGTG 
GAGAGCATCT TTGGTACAGA GCCCGAACCC CCTCTCGGAC CGTCCTCCGC CATGTCCAAG 
CTCCGGGGTG CAGTGGCTGC CGAGGGGGCC TCTGACACGG AGCGAGAGGA GCCCACAGAG 
AGCCAGGGCC TGGCTGCCCG GCTGCGGAGG CCATCCCCCC CAGAGCCCTG GGATGAGGAG 
GATGGGGCGT CTTGCAGCAC CTTCTTTGGC TCTGAGGAGC GGACGGTGAC CTGTGTGACT 
GTCGTGGAGC CGGAAGCCCC ACCCAGCCCG GACGTGCTGC AGGCTGCCAC CCACAGAGTC 
GTGGAGCTCA GGCCCCCTTC CCGGTCCCGC TCCACATCCA GCTCCCGCAG CAGGAAGAAG 
GCCAAGAGGA AGAGGGTGTC CAGGGAGCAC GGACGGACGC GCTCTGGGAC GCGCTCTGAA 
TCCAGGGACA GGAGCTCGAG GTCAGCGTCA CCATCAGTGG GTGAGGAGCG CCCCAGGAGG 
CAGCGGTCCA AGGCCAAGAG CCGGCGGTCC TCCAGTGACC GCTCCAGCAG CCGAGAGCGA 
GCTAAGAGGA AGAAAGCCAA GGACAAGAGC AGGGAGCACA GGCGGGGCCC CTGGGGCCAC 
AGCCGGAGGA CGTCCCGGTC GCGGTCGGGG AGCCCTGGCA GCTCTTCCTA TGAGCACTAT 
GAGAGTAGGA AGAAGAAGAA AAGGAGATCA GCGTCCAGAC CTCGGGGAAG GGAGTGCTCC 
CCCACCAGCA GCCTGGAGAG GCTCTGCAGG CACAAGCATC AGCGGGAACG CAGCCACGAG 
CGGCCAGACA GGAAGGAGAG TGTGGCGTGG CCCCGAGACC GGAGGAAGCG GAGGTCCCGG 
TCCCCAAGCT CGGAGCACAG GGCACGGGAG CACAGGCGGC CTCGGTCCCG TGAGAAGTGG 
CCGCAGACCC GGTCCCATTC CCCAGAGAGG AAGGGGGCTG TGAGGGAGGC TTCCCCAGCG 
CCCCTTGCAC AGGGGGAGCC AGGGCGGGAA GACCTCCCCA CCAGGTTGCC AGCCTTGGGG 
GAAGCACATG TCTCGCCGGA GGTGGCTACG GCCGACAAGG CCCCCCTGCA GGCTCCCCCT 
GTCCTGGAGG TGGCAGCTGA GTGTGAGCCG GACGACCTGG ACCTGGATTA TGGCGACTCC 
GTGGAGGCCG GACACGTCTT TGATGATTTC TCAAGCGACG CCGTTTTCAT CCAGCTCGAT 
GACATGAGCT CGCCACCTTC TCCCGAAAGC ACAGACTCTT CCCCGGAGCG AGACTTCCCA 
CTGAAGCCTG CGTTGCCCCC AGCCAGCCTG GCCGTGGCCG CCATCCAGAG GGAGGTGTCA 
TTGATGCACG ATGAAGACCC TTCGCAGCCC CCACCCCTGC CAGAGGGCAC CCAGGAGCCA 
CATTTGCTCA GGCCGGACGC GGCTGAGAAG GCTGAGGCAC CCAGTTCCCC GGATGTGGCG 
CCTGCGGGGA AGGAAGACAG CCCCTCTGCG AGTGGGAGGG TACAGGAGGC AGCCCGGCCT 
GAGGAGGTGG TTTCGCAGAC CCCCCTGCTG CGGTCCAGAG CCCTGGTGAA GCGGGTCACC 
TGGAACCTGC AGGAGTCGGA GAGCAGCGCC CCCGCCGAGG ACAGAGCCCC CCGGGCACCA 
CTTCACAGGC CACAGAAGCC CCGAGAAGGA GCCTGGGACA TGGAGGATGT GGCCCCCACA 
GGGGTCAGGC AGGTGTTCTC CGAGCTGCCC TTTCCCAGTC ACGTGCTTCC GGAACCCGGG 
TTCCCAGACA CAGACCCCTC TCAGGTTTAC AGCCCCGGCC TGCCGCCTGC CCCGGCCCAG 
CCCTCAAGCA TCCCACCCTG CGCACTGGTC AGCCAGCCCA CGGTCCAGTT CATCCTTCAG 
GGGAGCCTGC CGCTAGTGGG CTGTGGGGCA GCACAGACCC TGGCCCCAGT GCCCGCTGCC 
CTGACCCCAG CCTCAGAGCC AGCCAGTCAA GCCACTGCAG CCAGCAACTC GGAGGAGAAG 
ACCCCGGCCC CCAGGCTAGC TGCGGAGAAA ACCAAGAAGG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGTGCTGT GGAGGAGGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CCAAGGAGGA GTACAAGGAC ATCCTGCGCA AGGCCGTGCA GAAGATCTGC 
CACAGCAAGA GTGGAGAGAT CAACCCCGTG AAGGTGGCCA ACCTGGTGAA GGCGTACGTG 
GACAAGTACA GGCACATGCG CAGGCACAAG AAACCAGAGG CCGGGGAGGA GCCGCCCACG 
CAGGGGGCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273512.1
CDS	169..5106
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286583.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286583.1

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAAGC AGCGTGGGCA GCAGTGGGGA CTCTGGGGAC 
GACAGTGACA GCGAGCATGG AGATGGCACA GACGGAGAAG ACGAGGGGGC GTCTGAGGAG 
GAAGACCTGG AAGACAGATC TGGTTCCGAG GATTCTGAAG ACGACGGGGA GACATTGCTG 
GAGGTAGCGG GTACTCAGGG GAAACTGGAA GCCGCTGGCT CTTTCAATTC TGATGATGAT 
GCAGAGAGCT GCCCAATCTG TCTCAACGCA TTCAGAGACC AGGCCGTGGG GACGCCGGAG 
AACTGTGCCC ATTACTTCTG CCTGGACTGC ATTGTCGAAT GGTCCAAGAA TGCCAATTCC 
TGTCCAGTTG ATCGAACTCT ATTTAAGTGC ATTTGTATTC GAGCTCAATT TGGTGGTAAA 
ATCTTAAGAA AGATCCCAGT GGAGAACACC AAAGCGAGCG AGGAGGAGGA GGACCCGACC 
TTCTGTGAGG TGTGCGGCAG GAGCGACCGT GAGGACAGGC TTTTGCTCTG CGACGGCTGC 
GATGCGGGGT ACCACATGGA ATGCTTGGAC CCCCCTCTCC AGGAGGTGCC GGTGGACGAG 
TGGTTCTGCC CGGAATGTGC TGCGCCTGGT GTTGTCCTTG CCGCTGATGC GGGTCCCGTG 
AGTGAGGAGG AGGTCTCCCT GCTCTTGGCT GATGTGGTGC CCACCACCAG CAGGCTTCGG 
CCTCGAGCAG GTAGGACCCG GGCGATAGCC AGGACACGGC AGAGTGAGAG AGTGAGAGCA 
ACCGTGAACC GGAACCGGAT CTCCACGGCC AGGAGGGTCC AGCACACACC AGGGCGCCTC 
GGGTCTTCCC TGCTGGATGA AGCCATCGAG GCTGTGGCGA CTGGCCTGAG CACTGCCGTG 
TATCAGCGCC CCCTGACGCC GCGCACTCCC GCCCGACGGA AGAGGAAGAC AAGAAGACGG 
AAGAAAGTGC CGGGAAGAAA GAAAACCCCG TCCGGACCAT CCGCAAAAAG TAAGAGCTCA 
GCGACAAGAT CTAAGAAACG CCAACATCGA GTGAAGAAGA GAAGAGGGAA GAAGGTAAAG 
AGTGAAGCCA CCACTCGCTC TCGAATCGCG CGGACGCTGG GCCTGCGCAG GCCTGTTCAC 
AGCAGCTGCA TCCCGTCAGT GTTGAAGCCA GTGGAGCCCT CTTTGGGGCT GCTGAGAGCG 
GATATTGGAG CTGCCTCTCT GTCTCTGTTT GGAGATCCTT ATGAGCTGGA TCCCTTCGAC 
AGCAGTGAAG AGCTTTCTGC AAACCCTCTT TCCCCTCTGA GTGCCAAGAG ACGGGCTCTG 
TCCCGGTCAG CCCTGCAGTC CCACCAGCCC GTGGCCAGGC CCGTCTCCGT GGGGCTTTCC 
AGGAGGCGCC TCCCTGCCGC GGTGCCAGAG CCAGACTTGG AGGAGGAGCC AGTGCCTGAC 
CTGCTGGGCA GCATCCTGTC GGGCCAGAGC CTCCTGATGC TGGGCAGCAG TGATGTCATC 
ATCCACCGCG ACGGCTCCCT CAGCGCCAAG AGGGCGGCTC CAGTTTCTTT TCAGCGAAAC 
TCAGGCAGTC TGTCCAGAGG GGAAGAAGGA TTCAAGGGCT GCCTGCAGCC CCGAGCACTG 
CCCTCCGGGA GCCCGGCCCA AGGCCCGTCA GGAAACAGGC CACAGAGCAC AGGGCTCAGC 
TGTCAAGGCA GGTCCCGCAC CCCCGCCCGC ACCGCGGGGG CGCCTGTGAG GCTGGACTTG 
CCAGCAGCCC CTGGGGCGGT TCAGGCTCGG AACTTGTCAA ATGGGAGTGT GCCTGGCTTC 
AGACAGAGCC ACAGCCCCTG GTTCAACGGC ACCAACAAGC ACACCTTGCC CCTTGCCTCT 
GCCGCGTCTA AGATCTCAAG CAGAGATTCT AAGCCCCCAT GTCGCAGTGT GGTGCCGGGG 
CCTCCCCTGA AGCCAGCGCC CAGAAGAACA GACATCTCTG AGCTACCCAG GATACCAAAG 
ATCAGGAGAG ATGACGGTGG TGGCAGACGG GATGCGGCCC CGGCCCACGG GCAGAGCATT 
GAGATCCCCA GTGCCTGCAT CAGCCGACTG ACTGGCAGGG AGGGCACCGG GCAGCCAGGG 
CGAGGCACAC GGGCAGAGAG CGAGGCCAGC AGCAGGGTGC CCCGGGAGCC CGGGGTGCAC 
ACGGGCAGCT CCCGGCCCCC AGCCCCCAGC TCCCATGGCA GTTTGGCCCC ACTGGGACCA 
TCAAGAGGGA AAGGGGTCGG GTCGACCTTT GAGAGCTTCC GGATCAATAT TCCTGGAAAC 
ATGGCACATT CCAGCCAGCT CTCCAGCCCT GGCTTCTGTA ACACGTTCCG GCCTGTGGAC 
GATAAGGAGC AGAGGAAGGA GAACCCCTCA CCCCTCTTCT CCATCAAGAA GACGAAGCAG 
CTGCGGAGCG AGGTCTACGA CCCATCCGAC CCCACCGGCT CCGACTCCAG CGCCCCTGGC 
AGCAGCCCCG AGAGGTCTGG CCCCGGCCTC CTGCCCTCTG AGATCACACG AACCATCTCC 
ATCAACAGCC CGAAGGCCCA GACGGTGCAG GCTGTGCGCT GCGTCACCTC CTACACGGTG 
GAGAGCATCT TTGGTACAGA GCCCGAACCC CCTCTCGGAC CGTCCTCCGC CATGTCCAAG 
CTCCGGGGTG CAGTGGCTGC CGAGGGGGCC TCTGACACGG AGCGAGAGGA GCCCACAGAG 
AGCCAGGGCC TGGCTGCCCG GCTGCGGAGG CCATCCCCCC CAGAGCCCTG GGATGAGGAG 
GATGGGGCGT CTTGCAGCAC CTTCTTTGGC TCTGAGGAGC GGACGGTGAC CTGTGTGACT 
GTCGTGGAGC CGGAAGCCCC ACCCAGCCCG GACGTGCTGC AGGCTGCCAC CCACAGAGTC 
GTGGAGCTCA GGCCCCCTTC CCGGTCCCGC TCCACATCCA GCTCCCGCAG CAGGAAGAAG 
GCCAAGAGGA AGAGGGTGTC CAGGGAGCAC GGACGGACGC GCTCTGGGAC GCGCTCTGAA 
TCCAGGGACA GGAGCTCGAG GTCAGCGTCA CCATCAGTGG GTGAGGAGCG CCCCAGGAGG 
CAGCGGTCCA AGGCCAAGAG CCGGCGGTCC TCCAGTGACC GCTCCAGCAG CCGAGAGCGA 
GCTAAGAGGA AGAAAGCCAA GGACAAGAGC AGGGAGCACA GGCGGGGCCC CTGGGGCCAC 
AGCCGGAGGA CGTCCCGGTC GCGGTCGGGG AGCCCTGGCA GCTCTTCCTA TGAGCACTAT 
GAGAGTAGGA AGAAGAAGAA AAGGAGATCA GCGTCCAGAC CTCGGGGAAG GGAGTGCTCC 
CCCACCAGCA GCCTGGAGAG GCTCTGCAGG CACAAGCATC AGCGGGAACG CAGCCACGAG 
CGGCCAGACA GGAAGGAGAG TGTGGCGTGG CCCCGAGACC GGAGGAAGCG GAGGTCCCGG 
TCCCCAAGCT CGGAGCACAG GGCACGGGAG CACAGGCGGC CTCGGTCCCG TGAGAAGTGG 
CCGCAGACCC GGTCCCATTC CCCAGAGAGG AAGGGGGCTG TGAGGGAGGC TTCCCCAGCG 
CCCCTTGCAC AGGGGGAGCC AGGGCGGGAA GACCTCCCCA CCAGGTTGCC AGCCTTGGGG 
GAAGCACATG TCTCGCCGGA GGTGGCTACG GCCGACAAGG CCCCCCTGCA GGCTCCCCCT 
GTCCTGGAGG TGGCAGCTGA GTGTGAGCCG GACGACCTGG ACCTGGATTA TGGCGACTCC 
GTGGAGGCCG GACACGTCTT TGATGATTTC TCAAGCGACG CCGTTTTCAT CCAGCTCGAT 
GACATGAGCT CGCCACCTTC TCCCGAAAGC ACAGACTCTT CCCCGGAGCG AGACTTCCCA 
CTGAAGCCTG CGTTGCCCCC AGCCAGCCTG GCCGTGGCCG CCATCCAGAG GGAGGTGTCA 
TTGATGCACG ATGAAGACCC TTCGCAGCCC CCACCCCTGC CAGAGGGCAC CCAGGAGCCA 
CATTTGCTCA GGCCGGACGC GGCTGAGAAG GCTGAGGCAC CCAGTTCCCC GGATGTGGCG 
CCTGCGGGGA AGGAAGACAG CCCCTCTGCG AGTGGGAGGG TACAGGAGGC AGCCCGGCCT 
GAGGAGGTGG TTTCGCAGAC CCCCCTGCTG CGGTCCAGAG CCCTGGTGAA GCGGGTCACC 
TGGAACCTGC AGGAGTCGGA GAGCAGCGCC CCCGCCGAGG ACAGAGCCCC CCGGGCACCA 
CTTCACAGGC CACAGAAGCC CCGAGAAGGA GCCTGGGACA TGGAGGATGT GGCCCCCACA 
GGGGTCAGGC AGGTGTTCTC CGAGCTGCCC TTTCCCAGTC ACGTGCTTCC GGAACCCGGG 
TTCCCAGACA CAGACCCCTC TCAGGTTTAC AGCCCCGGCC TGCCGCCTGC CCCGGCCCAG 
CCCTCAAGCA TCCCACCCTG CGCACTGGTC AGCCAGCCCA CGGTCCAGTT CATCCTTCAG 
GGGAGCCTGC CGCTAGTGGG CTGTGGGGCA GCACAGACCC TGGCCCCAGT GCCCGCTGCC 
CTGACCCCAG CCTCAGAGCC AGCCAGTCAA GCCACTGCAG CCAGCAACTC GGAGGAGAAG 
ACCCCGGCCC CCAGGCTAGC TGCGGAGAAA ACCAAGAAGG AGGAGTACAT GAAGAAGCTG 
CACATGCAGG AGCGTGCTGT GGAGGAGGTG AAGCTGGCCA TCAAGCCCTT CTACCAGAAG 
AGGGAGGTGA CCAAGGAGGA GTACAAGGAC ATCCTGCGCA AGGCCGTGCA GAAGATCTGC 
CACAGCAAGA GTGGAGAGAT CAACCCCGTG AAGGTGGCCA ACCTGGTGAA GGCGTACGTG 
GACAAGTACA GGCACATGCG CAGGCACAAG AAACCAGAGG CCGGGGAGGA GCCGCCCACG 
CAGGGGGCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286581.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273510.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.

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CloneID	OHu28192
Clone ID Related Accession (Same CDS sequence)	NM_001286581.1 , NM_001286581.2
Accession Version	NM_001286581.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC146771.1 and AP006284.2. On Nov 8, 2013 this sequence version replaced XM_005253024.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146771.1, AB040975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273510.1
CDS	169..5118
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286581.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286581.1

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286581.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273510.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.

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CloneID	OHu28192
Clone ID Related Accession (Same CDS sequence)	NM_001286581.1 , NM_001286581.2
Accession Version	NM_001286581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC146771.1 and AP006284.2. On Nov 22, 2018 this sequence version replaced NM_001286581.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146771.1, AB040975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264555.10/ ENSP00000264555.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273510.1
CDS	145..5094
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286581.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286581.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286582.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273511.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.

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CloneID	OHu28216
Clone ID Related Accession (Same CDS sequence)	NM_001286582.2 , NM_001286582.1
Accession Version	NM_001286582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144295.1, BC144293.1 and BC146771.1. On May 31, 2019 this sequence version replaced NM_001286582.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273511.1
CDS	145..5088
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286582.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286582.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( NM_001286582.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273511.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.

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CloneID	OHu28216
Clone ID Related Accession (Same CDS sequence)	NM_001286582.2 , NM_001286582.1
Accession Version	NM_001286582.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA656880.1, BC144295.1, BC144293.1 and BC146771.1. On Nov 8, 2013 this sequence version replaced XM_005253026.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273511.1
CDS	169..5112
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	NM_001286582.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286582.1

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005253025.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253082.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu44926
Clone ID Related Accession (Same CDS sequence)	XM_005253025.5 , XM_005253025.4
Accession Version	XM_005253025.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253025.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GCAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253082.1
CDS	130..5076
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_005253025.5
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253025.5

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GCAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005253025.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253082.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

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CloneID	OHu44926
Clone ID Related Accession (Same CDS sequence)	XM_005253025.5 , XM_005253025.4
Accession Version	XM_005253025.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253025.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GCAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253082.1
CDS	130..5076
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_005253025.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253025.4

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAAGCA GCGTGGGCAG CAGTGGGGAC 
TCTGGGGACG ACAGTGACAG CGAGCATGGA GATGGCACAG ACGGAGAAGA CGAGGGGGCG 
TCTGAGGAGG AAGACCTGGA AGACAGATCT GGTTCCGAGG ATTCTGAAGA CGACGGGGAG 
ACATTGCTGG AGGTAGCGGG TACTCAGGGG AAACTGGAAG CCGCTGGCTC TTTCAATTCT 
GATGATGATG CAGAGAGCTG CCCAATCTGT CTCAACGCAT TCAGAGACCA GGCCGTGGGG 
ACGCCGGAGA ACTGTGCCCA TTACTTCTGC CTGGACTGCA TTGTCGAATG GTCCAAGAAT 
GCCAATTCCT GTCCAGTTGA TCGAACTCTA TTTAAGTGCA TTTGTATTCG AGCTCAATTT 
GGTGGTAAAA TCTTAAGAAA GATCCCAGTG GAGAACACCA AAGCGAGCGA GGAGGAGGAG 
GACCCGACCT TCTGTGAGGT GTGCGGCAGG AGCGACCGTG AGGACAGGCT TTTGCTCTGC 
GACGGCTGCG ATGCGGGGTA CCACATGGAA TGCTTGGACC CCCCTCTCCA GGAGGTGCCG 
GTGGACGAGT GGTTCTGCCC GGAATGTGCT GCGCCTGGTG TTGTCCTTGC CGCTGATGCG 
GGTCCCGTGA GTGAGGAGGA GGTCTCCCTG CTCTTGGCTG ATGTGGTGCC CACCACCAGC 
AGGCTTCGGC CTCGAGCAGG TAGGACCCGG GCGATAGCCA GGACACGGCA GAGTGAGAGA 
GTGAGAGCAA CCGTGAACCG GAACCGGATC TCCACGGCCA GGAGGGTCCA GCACACACCA 
GGGCGCCTCG GGTCTTCCCT GCTGGATGAA GCCATCGAGG CTGTGGCGAC TGGCCTGAGC 
ACTGCCGTGT ATCAGCGCCC CCTGACGCCG CGCACTCCCG CCCGACGGAA GAGGAAGACA 
AGAAGACGGA AGAAAGTGCC GGGAAGAAAG AAAACCCCGT CCGGACCATC CGCAAAAAGT 
AAGAGCTCAG CGACAAGATC TAAGAAACGC CAACATCGAG TGAAGAAGAG AAGAGGGAAG 
AAGGTAAAGA GTGAAGCCAC CACTCGCTCT CGAATCGCGC GGACGCTGGG CCTGCGCAGG 
CCTGTTCACA GCAGCTGCAT CCCGTCAGTG TTGAAGCCAG TGGAGCCCTC TTTGGGGCTG 
CTGAGAGCGG ATATTGGAGC TGCCTCTCTG TCTCTGTTTG GAGATCCTTA TGAGCTGGAT 
CCCTTCGACA GCAGTGAAGA GCTTTCTGCA AACCCTCTTT CCCCTCTGAG TGCCAAGAGA 
CGGGCTCTGT CCCGGTCAGC CCTGCAGTCC CACCAGCCCG TGGCCAGGCC CGTCTCCGTG 
GGGCTTTCCA GGAGGCGCCT CCCTGCCGCG GTGCCAGAGC CAGACTTGGA GGAGGAGCCA 
GTGCCTGACC TGCTGGGCAG CATCCTGTCG GGCCAGAGCC TCCTGATGCT GGGCAGCAGT 
GATGTCATCA TCCACCGCGA CGGCTCCCTC AGCGCCAAGA GGGCGGCTCC AGTTTCTTTT 
CAGCGAAACT CAGGCAGTCT GTCCAGAGGG GAAGAAGGAT TCAAGGGCTG CCTGCAGCCC 
CGAGCACTGC CCTCCGGGAG CCCGGCCCAA GGCCCGTCAG GAAACAGGCC ACAGAGCACA 
GGGCTCAGCT GTCAAGGCAG GTCCCGCACC CCCGCCCGCA CCGCGGGGGC GCCTGTGAGG 
CTGGACTTGC CAGCAGCCCC TGGGGCGGTT CAGGCTCGGA ACTTGTCAAA TGGGAGTGTG 
CCTGGCTTCA GACAGAGCCA CAGCCCCTGG TTCAACGGCA CCAACAAGCA CACCTTGCCC 
CTTGCCTCTG CCGCGTCTAA GATCTCAAGC AGAGATTCTA AGCCCCCATG TCGCAGTGTG 
GTGCCGGGGC CTCCCCTGAA GCCAGCGCCC AGAAGAACAG ACATCTCTGA GCTACCCAGG 
ATACCAAAGA TCAGGAGAGA TGACGGTGGT GGCAGACGGG ATGCGGCCCC GGCCCACGGG 
CAGAGCATTG AGATCCCCAG TGCCTGCATC AGCCGACTGA CTGGCAGGGA GGGCACCGGG 
CAGCCAGGGC GAGGCACACG GGCAGAGAGC GAGGCCAGCA GCAGGGTGCC CCGGGAGCCC 
GGGGTGCACA CGGGCAGCTC CCGGCCCCCA GCCCCCAGCT CCCATGGCAG TTTGGCCCCA 
CTGGGACCAT CAAGAGGGAA AGGGGTCGGG TCGACCTTTG AGAGCTTCCG GATCAATATT 
CCTGGAAACA TGGCACATTC CAGCCAGCTC TCCAGCCCTG GCTTCTGTAA CACGTTCCGG 
CCTGTGGACG ATAAGGAGCA GAGGAAGGAG AACCCCTCAC CCCTCTTCTC CATCAAGAAG 
ACGAAGCAGC TGCGGAGCGA GGTCTACGAC CCATCCGACC CCACCGGCTC CGACTCCAGC 
GCCCCTGGCA GCAGCCCCGA GAGGTCTGGC CCCGGCCTCC TGCCCTCTGA GATCACACGA 
ACCATCTCCA TCAACAGCCC GAAGGCCCAG ACGGTGCAGG CTGTGCGCTG CGTCACCTCC 
TACACGGTGG AGAGCATCTT TGGTACAGAG CCCGAACCCC CTCTCGGACC GTCCTCCGCC 
ATGTCCAAGC TCCGGGGTGC AGTGGCTGCC GAGGGGGCCT CTGACACGGA GCGAGAGGAG 
CCCACAGAGA GCCAGGGCCT GGCTGCCCGG CTGCGGAGGC CATCCCCCCC AGAGCCCTGG 
GATGAGGAGG ATGGGGCGTC TTGCAGCACC TTCTTTGGCT CTGAGGAGCG GACGGTGACC 
TGTGTGACTG TCGTGGAGCC GGAAGCCCCA CCCAGCCCGG ACGTGCTGCA GGCTGCCACC 
CACAGAGTCG TGGAGCTCAG GCCCCCTTCC CGGTCCCGCT CCACATCCAG CTCCCGCAGC 
AGGAAGAAGG CCAAGAGGAA GAGGGTGTCC AGGGAGCACG GACGGACGCG CTCTGGGACG 
CGCTCTGAAT CCAGGGACAG GAGCTCGAGG TCAGCGTCAC CATCAGTGGG TGAGGAGCGC 
CCCAGGAGGC AGCGGTCCAA GGCCAAGAGC CGGCGGTCCT CCAGTGACCG CTCCAGCAGC 
CGAGAGCGAG CTAAGAGGAA GAAAGCCAAG GACAAGAGCA GGGAGCACAG GCGGGGCCCC 
TGGGGCCACA GCCGGAGGAC GTCCCGGTCG CGGTCGGGGA GCCCTGGCAG CTCTTCCTAT 
GAGCACTATG AGAGTAGGAA GAAGAAGAAA AGGAGATCAG CGTCCAGACC TCGGGGAAGG 
GAGTGCTCCC CCACCAGCAG CCTGGAGAGG CTCTGCAGGC ACAAGCATCA GCGGGAACGC 
AGCCACGAGC GGCCAGACAG GAAGGAGAGT GTGGCGTGGC CCCGAGACCG GAGGAAGCGG 
AGGTCCCGGT CCCCAAGCTC GGAGCACAGG GCACGGGAGC ACAGGCGGCC TCGGTCCCGT 
GAGAAGTGGC CGCAGACCCG GTCCCATTCC CCAGAGAGGA AGGGGGCTGT GAGGGAGGCT 
TCCCCAGCGC CCCTTGCACA GGGGGAGCCA GGGCGGGAAG ACCTCCCCAC CAGGTTGCCA 
GCCTTGGGGG AAGCACATGT CTCGCCGGAG GTGGCTACGG CCGACAAGGC CCCCCTGCAG 
GCTCCCCCTG TCCTGGAGGT GGCAGCTGAG TGTGAGCCGG ACGACCTGGA CCTGGATTAT 
GGCGACTCCG TGGAGGCCGG ACACGTCTTT GATGATTTCT CAAGCGACGC CGTTTTCATC 
CAGCTCGATG ACATGAGCTC GCCACCTTCT CCCGAAAGCA CAGACTCTTC CCCGGAGCGA 
GACTTCCCAC TGAAGCCTGC GTTGCCCCCA GCCAGCCTGG CCGTGGCCGC CATCCAGAGG 
GAGGTGTCAT TGATGCACGA TGAAGACCCT TCGCAGCCCC CACCCCTGCC AGAGGGCACC 
CAGGAGCCAC ATTTGCTCAG GCCGGACGCG GCTGAGAAGG CTGAGGCACC CAGTTCCCCG 
GATGTGGCGC CTGCGGGGAA GGAAGACAGC CCCTCTGCGA GTGGGAGGGT ACAGGAGGCA 
GCCCGGCCTG AGGAGGTGGT TTCGCAGACC CCCCTGCTGC GGTCCAGAGC CCTGGTGAAG 
CGGGTCACCT GGAACCTGCA GGAGTCGGAG AGCAGCGCCC CCGCCGAGGA CAGAGCCCCC 
CGGGCACCAC TTCACAGGCC ACAGAAGCCC CGAGAAGGAG CCTGGGACAT GGAGGATGTG 
GCCCCCACAG GGGTCAGGCA GGTGTTCTCC GAGCTGCCCT TTCCCAGTCA CGTGCTTCCG 
GAACCCGGGT TCCCAGACAC AGACCCCTCT CAGGTTTACA GCCCCGGCCT GCCGCCTGCC 
CCGGCCCAGC CCTCAAGCAT CCCACCCTGC GCACTGGTCA GCCAGCCCAC GGTCCAGTTC 
ATCCTTCAGG GGAGCCTGCC GCTAGTGGGC TGTGGGGCAG CACAGACCCT GGCCCCAGTG 
CCCGCTGCCC TGACCCCAGC CTCAGAGCCA GCCAGTCAAG CCACTGCAGC CAGCAACTCG 
GAGGAGAAGA CCCCGGCCCC CAGGCTAGCT GCGGAGAAAA CCAAGAAGGA GGAGTACATG 
AAGAAGCTGC ACATGCAGGA GCGTGCTGTG GAGGAGGTGA AGCTGGCCAT CAAGCCCTTC 
TACCAGAAGA GGGAGGTGAC CAAGGAGGAG TACAAGGACA TCCTGCGCAA GGCCGTGCAG 
AAGATCTGCC ACAGCAAGAG TGGAGAGATC AACCCCGTGA AGGTGGCCAA CCTGGTGAAG 
GCGTACGTGG ACAAGTACAG GCACATGCGC AGGCACAAGA AACCAGAGGC CGGGGAGGAG 
CCGCCCACGC AGGGGGCCGA GGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_005253027.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253084.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

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CloneID	OHu44927
Clone ID Related Accession (Same CDS sequence)	XM_005253027.3
Accession Version	XM_005253027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAGAA AGCAGCGTGG GCAGCAGTGG GGACTCTGGG 
GACGACAGTG ACAGCGAGCA TGGAGATGGC ACAGACGGAG AAGACGAGGG GGCGTCTGAG 
GAGGAAGACC TGGAAGACAG ATCTGGTTCC GAGGATTCTG AAGACGACGG GGAGACATTG 
CTGGAGGTAG CGGGTACTCA GGGGAAACTG GAAGCCGCTG GCTCTTTCAA TTCTGATGAT 
GATGCAGAGA GCTGCCCAAT CTGTCTCAAC GCATTCAGAG ACCAGGCCGT GGGGACGCCG 
GAGAACTGTG CCCATTACTT CTGCCTGGAC TGCATTGTCG AATGGTCCAA GAATGCCAAT 
TCCTGTCCAG TTGATCGAAC TCTATTTAAG TGCATTTGTA TTCGAGCTCA ATTTGGTGGT 
AAAATCTTAA GAAAGATCCC AGTGGAGAAC ACCAAAGCGA GCGAGGAGGA GGAGGACCCG 
ACCTTCTGTG AGGTGTGCGG CAGGAGCGAC CGTGAGGACA GGCTTTTGCT CTGCGACGGC 
TGCGATGCGG GGTACCACAT GGAATGCTTG GACCCCCCTC TCCAGGAGGT GCCGGTGGAC 
GAGTGGTTCT GCCCGGAATG TGCTGCGCCT GGTGTTGTCC TTGCCGCTGA TGCGGGTCCC 
GTGAGTGAGG AGGAGGTCTC CCTGCTCTTG GCTGATGTGG TGCCCACCAC CAGCAGGCTT 
CGGCCTCGAG CAGGTAGGAC CCGGGCGATA GCCAGGACAC GGCAGAGTGA GAGAGTGAGA 
GCAACCGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGG TCCAGCACAC ACCAGGGCGC 
CTCGGGTCTT CCCTGCTGGA TGAAGCCATC GAGGCTGTGG CGACTGGCCT GAGCACTGCC 
GTGTATCAGC GCCCCCTGAC GCCGCGCACT CCCGCCCGAC GGAAGAGGAA GACAAGAAGA 
CGGAAGAAAG TGCCGGGAAG AAAGAAAACC CCGTCCGGAC CATCCGCAAA AAGTAAGAGC 
TCAGCGACAA GATCTAAGAA ACGCCAACAT CGAGTGAAGA AGAGAAGAGG GAAGAAGGTA 
AAGAGTGAAG CCACCACTCG CTCTCGAATC GCGCGGACGC TGGGCCTGCG CAGGCCTGTT 
CACAGCAGCT GCATCCCGTC AGTGTTGAAG CCAGTGGAGC CCTCTTTGGG GCTGCTGAGA 
GCGGATATTG GAGCTGCCTC TCTGTCTCTG TTTGGAGATC CTTATGAGCT GGATCCCTTC 
GACAGCAGTG AAGAGCTTTC TGCAAACCCT CTTTCCCCTC TGAGTGCCAA GAGACGGGCT 
CTGTCCCGGT CAGCCCTGCA GTCCCACCAG CCCGTGGCCA GGCCCGTCTC CGTGGGGCTT 
TCCAGGAGGC GCCTCCCTGC CGCGGTGCCA GAGCCAGACT TGGAGGAGGA GCCAGTGCCT 
GACCTGCTGG GCAGCATCCT GTCGGGCCAG AGCCTCCTGA TGCTGGGCAG CAGTGATGTC 
ATCATCCACC GCGACGGCTC CCTCAGCGCC AAGAGGGCGG CTCCAGTTTC TTTTCAGCGA 
AACTCAGGCA GTCTGTCCAG AGGGGAAGAA GGATTCAAGG GCTGCCTGCA GCCCCGAGCA 
CTGCCCTCCG GGAGCCCGGC CCAAGGCCCG TCAGGAAACA GGCCACAGAG CACAGGGCTC 
AGCTGTCAAG GCAGGTCCCG CACCCCCGCC CGCACCGCGG GGGCGCCTGT GAGGCTGGAC 
TTGCCAGCAG CCCCTGGGGC GGTTCAGGCT CGGAACTTGT CAAATGGGAG TGTGCCTGGC 
TTCAGACAGA GCCACAGCCC CTGGTTCAAC GGCACCAACA AGCACACCTT GCCCCTTGCC 
TCTGCCGCGT CTAAGATCTC AAGCAGAGAT TCTAAGCCCC CATGTCGCAG TGTGGTGCCG 
GGGCCTCCCC TGAAGCCAGC GCCCAGAAGA ACAGACATCT CTGAGCTACC CAGGATACCA 
AAGATCAGGA GAGATGACGG TGGTGGCAGA CGGGATGCGG CCCCGGCCCA CGGGCAGAGC 
ATTGAGATCC CCAGTGCCTG CATCAGCCGA CTGACTGGCA GGGAGGGCAC CGGGCAGCCA 
GGGCGAGGCA CACGGGCAGA GAGCGAGGCC AGCAGCAGGG TGCCCCGGGA GCCCGGGGTG 
CACACGGGCA GCTCCCGGCC CCCAGCCCCC AGCTCCCATG GCAGTTTGGC CCCACTGGGA 
CCATCAAGAG GGAAAGGGGT CGGGTCGACC TTTGAGAGCT TCCGGATCAA TATTCCTGGA 
AACATGGCAC ATTCCAGCCA GCTCTCCAGC CCTGGCTTCT GTAACACGTT CCGGCCTGTG 
GACGATAAGG AGCAGAGGAA GGAGAACCCC TCACCCCTCT TCTCCATCAA GAAGACGAAG 
CAGCTGCGGA GCGAGGTCTA CGACCCATCC GACCCCACCG GCTCCGACTC CAGCGCCCCT 
GGCAGCAGCC CCGAGAGGTC TGGCCCCGGC CTCCTGCCCT CTGAGATCAC ACGAACCATC 
TCCATCAACA GCCCGAAGGC CCAGACGGTG CAGGCTGTGC GCTGCGTCAC CTCCTACACG 
GTGGAGAGCA TCTTTGGTAC AGAGCCCGAA CCCCCTCTCG GACCGTCCTC CGCCATGTCC 
AAGCTCCGGG GTGCAGTGGC TGCCGAGGGG GCCTCTGACA CGGAGCGAGA GGAGCCCACA 
GAGAGCCAGG GCCTGGCTGC CCGGCTGCGG AGGCCATCCC CCCCAGAGCC CTGGGATGAG 
GAGGATGGGG CGTCTTGCAG CACCTTCTTT GGCTCTGAGG AGCGGACGGT GACCTGTGTG 
ACTGTCGTGG AGCCGGAAGC CCCACCCAGC CCGGACGTGC TGCAGGCTGC CACCCACAGA 
GTCGTGGAGC TCAGGCCCCC TTCCCGGTCC CGCTCCACAT CCAGCTCCCG CAGCAGGAAG 
AAGGCCAAGA GGAAGAGGGT GTCCAGGGAG CACGGACGGA CGCGCTCTGG GACGCGCTCT 
GAATCCAGGG ACAGGAGCTC GAGGTCAGCG TCACCATCAG TGGGTGAGGA GCGCCCCAGG 
AGGCAGCGGT CCAAGGCCAA GAGCCGGCGG TCCTCCAGTG ACCGCTCCAG CAGCCGAGAG 
CGAGCTAAGA GGAAGAAAGC CAAGGACAAG AGCAGGGAGC ACAGGCGGGG CCCCTGGGGC 
CACAGCCGGA GGACGTCCCG GTCGCGGTCG GGGAGCCCTG GCAGCTCTTC CTATGAGCAC 
TATGAGAGTA GGAAGAAGAA GAAAAGGAGA TCAGCGTCCA GACCTCGGGG AAGGGAGTGC 
TCCCCCACCA GCAGCCTGGA GAGGCTCTGC AGGCACAAGC ATCAGCGGGA ACGCAGCCAC 
GAGCGGCCAG ACAGGAAGGA GAGTGTGGCG TGGCCCCGAG ACCGGAGGAA GCGGAGGTCC 
CGGTCCCCAA GCTCGGAGCA CAGGGCACGG GAGCACAGGC GGCCTCGGTC CCGTGAGAAG 
TGGCCGCAGA CCCGGTCCCA TTCCCCAGAG AGGAAGGGGG CTGTGAGGGA GGCTTCCCCA 
GCGCCCCTTG CACAGGGGGA GCCAGGGCGG GAAGACCTCC CCACCAGGTT GCCAGCCTTG 
GGGGAAGCAC ATGTCTCGCC GGAGGTGGCT ACGGCCGACA AGGCCCCCCT GCAGGCTCCC 
CCTGTCCTGG AGGTGGCAGC TGAGTGTGAG CCGGACGACC TGGACCTGGA TTATGGCGAC 
TCCGTGGAGG CCGGACACGT CTTTGATGAT TTCTCAAGCG ACGCCGTTTT CATCCAGCTC 
GATGACATGA GCTCGCCACC TTCTCCCGAA AGCACAGACT CTTCCCCGGA GCGAGACTTC 
CCACTGAAGC CTGCGTTGCC CCCAGCCAGC CTGGCCGTGG CCGCCATCCA GAGGGAGGTG 
TCATTGATGC ACGATGAAGA CCCTTCGCAG CCCCCACCCC TGCCAGAGGG CACCCAGGAG 
CCACATTTGC TCAGGCCGGA CGCGGCTGAG AAGGCTGAGG CACCCAGTTC CCCGGATGTG 
GCGCCTGCGG GGAAGGAAGA CAGCCCCTCT GCGAGTGGGA GGGTACAGGA GGCAGCCCGG 
CCTGAGGAGG TGGTTTCGCA GACCCCCCTG CTGCGGTCCA GAGCCCTGGT GAAGCGGGTC 
ACCTGGAACC TGCAGGAGTC GGAGAGCAGC GCCCCCGCCG AGGACAGAGC CCCCCGGGCA 
CCACTTCACA GGCCACAGAA GCCCCGAGAA GGAGCCTGGG ACATGGAGGA TGTGGCCCCC 
ACAGGGGTCA GGCAGGTGTT CTCCGAGCTG CCCTTTCCCA GTCACGTGCT TCCGGAACCC 
GGGTTCCCAG ACACAGACCC CTCTCAGGTT TACAGCCCCG GCCTGCCGCC TGCCCCGGCC 
CAGCCCTCAA GCATCCCACC CTGCGCACTG GTCAGCCAGC CCACGGTCCA GTTCATCCTT 
CAGGGGAGCC TGCCGCTAGT GGGCTGTGGG GCAGCACAGA CCCTGGCCCC AGTGCCCGCT 
GCCCTGACCC CAGCCTCAGA GCCAGCCAGT CAAGCCACTG CAGCCAGCAA CTCGGAGGAG 
AAGACCCCGG CCCCCAGGCT AGCTGCGGAG AAAACCAAGA AGGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGTGC TGTGGAGGAG GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACCAAGGA GGAGTACAAG GACATCCTGC GCAAGGCCGT GCAGAAGATC 
TGCCACAGCA AGAGTGGAGA GATCAACCCC GTGAAGGTGG CCAACCTGGT GAAGGCGTAC 
GTGGACAAGT ACAGGCACAT GCGCAGGCAC AAGAAACCAG AGGCCGGGGA GGAGCCGCCC 
ACGCAGGGGG CCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253084.1
CDS	124..5064
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_005253027.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253027.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGAAGAA AGCAGCGTGG GCAGCAGTGG GGACTCTGGG 
GACGACAGTG ACAGCGAGCA TGGAGATGGC ACAGACGGAG AAGACGAGGG GGCGTCTGAG 
GAGGAAGACC TGGAAGACAG ATCTGGTTCC GAGGATTCTG AAGACGACGG GGAGACATTG 
CTGGAGGTAG CGGGTACTCA GGGGAAACTG GAAGCCGCTG GCTCTTTCAA TTCTGATGAT 
GATGCAGAGA GCTGCCCAAT CTGTCTCAAC GCATTCAGAG ACCAGGCCGT GGGGACGCCG 
GAGAACTGTG CCCATTACTT CTGCCTGGAC TGCATTGTCG AATGGTCCAA GAATGCCAAT 
TCCTGTCCAG TTGATCGAAC TCTATTTAAG TGCATTTGTA TTCGAGCTCA ATTTGGTGGT 
AAAATCTTAA GAAAGATCCC AGTGGAGAAC ACCAAAGCGA GCGAGGAGGA GGAGGACCCG 
ACCTTCTGTG AGGTGTGCGG CAGGAGCGAC CGTGAGGACA GGCTTTTGCT CTGCGACGGC 
TGCGATGCGG GGTACCACAT GGAATGCTTG GACCCCCCTC TCCAGGAGGT GCCGGTGGAC 
GAGTGGTTCT GCCCGGAATG TGCTGCGCCT GGTGTTGTCC TTGCCGCTGA TGCGGGTCCC 
GTGAGTGAGG AGGAGGTCTC CCTGCTCTTG GCTGATGTGG TGCCCACCAC CAGCAGGCTT 
CGGCCTCGAG CAGGTAGGAC CCGGGCGATA GCCAGGACAC GGCAGAGTGA GAGAGTGAGA 
GCAACCGTGA ACCGGAACCG GATCTCCACG GCCAGGAGGG TCCAGCACAC ACCAGGGCGC 
CTCGGGTCTT CCCTGCTGGA TGAAGCCATC GAGGCTGTGG CGACTGGCCT GAGCACTGCC 
GTGTATCAGC GCCCCCTGAC GCCGCGCACT CCCGCCCGAC GGAAGAGGAA GACAAGAAGA 
CGGAAGAAAG TGCCGGGAAG AAAGAAAACC CCGTCCGGAC CATCCGCAAA AAGTAAGAGC 
TCAGCGACAA GATCTAAGAA ACGCCAACAT CGAGTGAAGA AGAGAAGAGG GAAGAAGGTA 
AAGAGTGAAG CCACCACTCG CTCTCGAATC GCGCGGACGC TGGGCCTGCG CAGGCCTGTT 
CACAGCAGCT GCATCCCGTC AGTGTTGAAG CCAGTGGAGC CCTCTTTGGG GCTGCTGAGA 
GCGGATATTG GAGCTGCCTC TCTGTCTCTG TTTGGAGATC CTTATGAGCT GGATCCCTTC 
GACAGCAGTG AAGAGCTTTC TGCAAACCCT CTTTCCCCTC TGAGTGCCAA GAGACGGGCT 
CTGTCCCGGT CAGCCCTGCA GTCCCACCAG CCCGTGGCCA GGCCCGTCTC CGTGGGGCTT 
TCCAGGAGGC GCCTCCCTGC CGCGGTGCCA GAGCCAGACT TGGAGGAGGA GCCAGTGCCT 
GACCTGCTGG GCAGCATCCT GTCGGGCCAG AGCCTCCTGA TGCTGGGCAG CAGTGATGTC 
ATCATCCACC GCGACGGCTC CCTCAGCGCC AAGAGGGCGG CTCCAGTTTC TTTTCAGCGA 
AACTCAGGCA GTCTGTCCAG AGGGGAAGAA GGATTCAAGG GCTGCCTGCA GCCCCGAGCA 
CTGCCCTCCG GGAGCCCGGC CCAAGGCCCG TCAGGAAACA GGCCACAGAG CACAGGGCTC 
AGCTGTCAAG GCAGGTCCCG CACCCCCGCC CGCACCGCGG GGGCGCCTGT GAGGCTGGAC 
TTGCCAGCAG CCCCTGGGGC GGTTCAGGCT CGGAACTTGT CAAATGGGAG TGTGCCTGGC 
TTCAGACAGA GCCACAGCCC CTGGTTCAAC GGCACCAACA AGCACACCTT GCCCCTTGCC 
TCTGCCGCGT CTAAGATCTC AAGCAGAGAT TCTAAGCCCC CATGTCGCAG TGTGGTGCCG 
GGGCCTCCCC TGAAGCCAGC GCCCAGAAGA ACAGACATCT CTGAGCTACC CAGGATACCA 
AAGATCAGGA GAGATGACGG TGGTGGCAGA CGGGATGCGG CCCCGGCCCA CGGGCAGAGC 
ATTGAGATCC CCAGTGCCTG CATCAGCCGA CTGACTGGCA GGGAGGGCAC CGGGCAGCCA 
GGGCGAGGCA CACGGGCAGA GAGCGAGGCC AGCAGCAGGG TGCCCCGGGA GCCCGGGGTG 
CACACGGGCA GCTCCCGGCC CCCAGCCCCC AGCTCCCATG GCAGTTTGGC CCCACTGGGA 
CCATCAAGAG GGAAAGGGGT CGGGTCGACC TTTGAGAGCT TCCGGATCAA TATTCCTGGA 
AACATGGCAC ATTCCAGCCA GCTCTCCAGC CCTGGCTTCT GTAACACGTT CCGGCCTGTG 
GACGATAAGG AGCAGAGGAA GGAGAACCCC TCACCCCTCT TCTCCATCAA GAAGACGAAG 
CAGCTGCGGA GCGAGGTCTA CGACCCATCC GACCCCACCG GCTCCGACTC CAGCGCCCCT 
GGCAGCAGCC CCGAGAGGTC TGGCCCCGGC CTCCTGCCCT CTGAGATCAC ACGAACCATC 
TCCATCAACA GCCCGAAGGC CCAGACGGTG CAGGCTGTGC GCTGCGTCAC CTCCTACACG 
GTGGAGAGCA TCTTTGGTAC AGAGCCCGAA CCCCCTCTCG GACCGTCCTC CGCCATGTCC 
AAGCTCCGGG GTGCAGTGGC TGCCGAGGGG GCCTCTGACA CGGAGCGAGA GGAGCCCACA 
GAGAGCCAGG GCCTGGCTGC CCGGCTGCGG AGGCCATCCC CCCCAGAGCC CTGGGATGAG 
GAGGATGGGG CGTCTTGCAG CACCTTCTTT GGCTCTGAGG AGCGGACGGT GACCTGTGTG 
ACTGTCGTGG AGCCGGAAGC CCCACCCAGC CCGGACGTGC TGCAGGCTGC CACCCACAGA 
GTCGTGGAGC TCAGGCCCCC TTCCCGGTCC CGCTCCACAT CCAGCTCCCG CAGCAGGAAG 
AAGGCCAAGA GGAAGAGGGT GTCCAGGGAG CACGGACGGA CGCGCTCTGG GACGCGCTCT 
GAATCCAGGG ACAGGAGCTC GAGGTCAGCG TCACCATCAG TGGGTGAGGA GCGCCCCAGG 
AGGCAGCGGT CCAAGGCCAA GAGCCGGCGG TCCTCCAGTG ACCGCTCCAG CAGCCGAGAG 
CGAGCTAAGA GGAAGAAAGC CAAGGACAAG AGCAGGGAGC ACAGGCGGGG CCCCTGGGGC 
CACAGCCGGA GGACGTCCCG GTCGCGGTCG GGGAGCCCTG GCAGCTCTTC CTATGAGCAC 
TATGAGAGTA GGAAGAAGAA GAAAAGGAGA TCAGCGTCCA GACCTCGGGG AAGGGAGTGC 
TCCCCCACCA GCAGCCTGGA GAGGCTCTGC AGGCACAAGC ATCAGCGGGA ACGCAGCCAC 
GAGCGGCCAG ACAGGAAGGA GAGTGTGGCG TGGCCCCGAG ACCGGAGGAA GCGGAGGTCC 
CGGTCCCCAA GCTCGGAGCA CAGGGCACGG GAGCACAGGC GGCCTCGGTC CCGTGAGAAG 
TGGCCGCAGA CCCGGTCCCA TTCCCCAGAG AGGAAGGGGG CTGTGAGGGA GGCTTCCCCA 
GCGCCCCTTG CACAGGGGGA GCCAGGGCGG GAAGACCTCC CCACCAGGTT GCCAGCCTTG 
GGGGAAGCAC ATGTCTCGCC GGAGGTGGCT ACGGCCGACA AGGCCCCCCT GCAGGCTCCC 
CCTGTCCTGG AGGTGGCAGC TGAGTGTGAG CCGGACGACC TGGACCTGGA TTATGGCGAC 
TCCGTGGAGG CCGGACACGT CTTTGATGAT TTCTCAAGCG ACGCCGTTTT CATCCAGCTC 
GATGACATGA GCTCGCCACC TTCTCCCGAA AGCACAGACT CTTCCCCGGA GCGAGACTTC 
CCACTGAAGC CTGCGTTGCC CCCAGCCAGC CTGGCCGTGG CCGCCATCCA GAGGGAGGTG 
TCATTGATGC ACGATGAAGA CCCTTCGCAG CCCCCACCCC TGCCAGAGGG CACCCAGGAG 
CCACATTTGC TCAGGCCGGA CGCGGCTGAG AAGGCTGAGG CACCCAGTTC CCCGGATGTG 
GCGCCTGCGG GGAAGGAAGA CAGCCCCTCT GCGAGTGGGA GGGTACAGGA GGCAGCCCGG 
CCTGAGGAGG TGGTTTCGCA GACCCCCCTG CTGCGGTCCA GAGCCCTGGT GAAGCGGGTC 
ACCTGGAACC TGCAGGAGTC GGAGAGCAGC GCCCCCGCCG AGGACAGAGC CCCCCGGGCA 
CCACTTCACA GGCCACAGAA GCCCCGAGAA GGAGCCTGGG ACATGGAGGA TGTGGCCCCC 
ACAGGGGTCA GGCAGGTGTT CTCCGAGCTG CCCTTTCCCA GTCACGTGCT TCCGGAACCC 
GGGTTCCCAG ACACAGACCC CTCTCAGGTT TACAGCCCCG GCCTGCCGCC TGCCCCGGCC 
CAGCCCTCAA GCATCCCACC CTGCGCACTG GTCAGCCAGC CCACGGTCCA GTTCATCCTT 
CAGGGGAGCC TGCCGCTAGT GGGCTGTGGG GCAGCACAGA CCCTGGCCCC AGTGCCCGCT 
GCCCTGACCC CAGCCTCAGA GCCAGCCAGT CAAGCCACTG CAGCCAGCAA CTCGGAGGAG 
AAGACCCCGG CCCCCAGGCT AGCTGCGGAG AAAACCAAGA AGGAGGAGTA CATGAAGAAG 
CTGCACATGC AGGAGCGTGC TGTGGAGGAG GTGAAGCTGG CCATCAAGCC CTTCTACCAG 
AAGAGGGAGG TGACCAAGGA GGAGTACAAG GACATCCTGC GCAAGGCCGT GCAGAAGATC 
TGCCACAGCA AGAGTGGAGA GATCAACCCC GTGAAGGTGG CCAACCTGGT GAAGGCGTAC 
GTGGACAAGT ACAGGCACAT GCGCAGGCAC AAGAAACCAG AGGCCGGGGA GGAGCCGCCC 
ACGCAGGGGG CCGAGGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_011520236.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518538.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OHu70810
Clone ID Related Accession (Same CDS sequence)	XM_011520236.3 , XM_011520236.2
Accession Version	XM_011520236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGAGTG AAGCCACCAC TCGCTCTCGA ATCGCGCGGA CGCTGGGCCT GCGCAGGCCT 
GTTCACAGCA GCTGCATCCC GTCAGTGTTG AAGCCAGTGG AGCCCTCTTT GGGGCTGCTG 
AGAGCGGATA TTGGAGCTGC CTCTCTGTCT CTGTTTGGAG ATCCTTATGA GCTGGATCCC 
TTCGACAGCA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518538.1
CDS	169..5112
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_011520236.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520236.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGAGTG AAGCCACCAC TCGCTCTCGA ATCGCGCGGA CGCTGGGCCT GCGCAGGCCT 
GTTCACAGCA GCTGCATCCC GTCAGTGTTG AAGCCAGTGG AGCCCTCTTT GGGGCTGCTG 
AGAGCGGATA TTGGAGCTGC CTCTCTGTCT CTGTTTGGAG ATCCTTATGA GCTGGATCCC 
TTCGACAGCA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_011520236.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518538.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

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CloneID	OHu70810
Clone ID Related Accession (Same CDS sequence)	XM_011520236.3 , XM_011520236.2
Accession Version	XM_011520236.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGAGTG AAGCCACCAC TCGCTCTCGA ATCGCGCGGA CGCTGGGCCT GCGCAGGCCT 
GTTCACAGCA GCTGCATCCC GTCAGTGTTG AAGCCAGTGG AGCCCTCTTT GGGGCTGCTG 
AGAGCGGATA TTGGAGCTGC CTCTCTGTCT CTGTTTGGAG ATCCTTATGA GCTGGATCCC 
TTCGACAGCA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518538.1
CDS	169..5112
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFEESSVGSSGDSGDDSDSEHGDGTDGEDEGASEEEDLEDRSGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_011520236.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520236.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGAAGAAA GCAGCGTGGG CAGCAGTGGG 
GACTCTGGGG ACGACAGTGA CAGCGAGCAT GGAGATGGCA CAGACGGAGA AGACGAGGGG 
GCGTCTGAGG AGGAAGACCT GGAAGACAGA TCTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGAGTG AAGCCACCAC TCGCTCTCGA ATCGCGCGGA CGCTGGGCCT GCGCAGGCCT 
GTTCACAGCA GCTGCATCCC GTCAGTGTTG AAGCCAGTGG AGCCCTCTTT GGGGCTGCTG 
AGAGCGGATA TTGGAGCTGC CTCTCTGTCT CTGTTTGGAG ATCCTTATGA GCTGGATCCC 
TTCGACAGCA GTGAAGAGCT TTCTGCAAAC CCTCTTTCCC CTCTGAGTGC CAAGAGACGG 
GCTCTGTCCC GGTCAGCCCT GCAGTCCCAC CAGCCCGTGG CCAGGCCCGT CTCCGTGGGG 
CTTTCCAGGA GGCGCCTCCC TGCCGCGGTG CCAGAGCCAG ACTTGGAGGA GGAGCCAGTG 
CCTGACCTGC TGGGCAGCAT CCTGTCGGGC CAGAGCCTCC TGATGCTGGG CAGCAGTGAT 
GTCATCATCC ACCGCGACGG CTCCCTCAGC GCCAAGAGGG CGGCTCCAGT TTCTTTTCAG 
CGAAACTCAG GCAGTCTGTC CAGAGGGGAA GAAGGATTCA AGGGCTGCCT GCAGCCCCGA 
GCACTGCCCT CCGGGAGCCC GGCCCAAGGC CCGTCAGGAA ACAGGCCACA GAGCACAGGG 
CTCAGCTGTC AAGGCAGGTC CCGCACCCCC GCCCGCACCG CGGGGGCGCC TGTGAGGCTG 
GACTTGCCAG CAGCCCCTGG GGCGGTTCAG GCTCGGAACT TGTCAAATGG GAGTGTGCCT 
GGCTTCAGAC AGAGCCACAG CCCCTGGTTC AACGGCACCA ACAAGCACAC CTTGCCCCTT 
GCCTCTGCCG CGTCTAAGAT CTCAAGCAGA GATTCTAAGC CCCCATGTCG CAGTGTGGTG 
CCGGGGCCTC CCCTGAAGCC AGCGCCCAGA AGAACAGACA TCTCTGAGCT ACCCAGGATA 
CCAAAGATCA GGAGAGATGA CGGTGGTGGC AGACGGGATG CGGCCCCGGC CCACGGGCAG 
AGCATTGAGA TCCCCAGTGC CTGCATCAGC CGACTGACTG GCAGGGAGGG CACCGGGCAG 
CCAGGGCGAG GCACACGGGC AGAGAGCGAG GCCAGCAGCA GGGTGCCCCG GGAGCCCGGG 
GTGCACACGG GCAGCTCCCG GCCCCCAGCC CCCAGCTCCC ATGGCAGTTT GGCCCCACTG 
GGACCATCAA GAGGGAAAGG GGTCGGGTCG ACCTTTGAGA GCTTCCGGAT CAATATTCCT 
GGAAACATGG CACATTCCAG CCAGCTCTCC AGCCCTGGCT TCTGTAACAC GTTCCGGCCT 
GTGGACGATA AGGAGCAGAG GAAGGAGAAC CCCTCACCCC TCTTCTCCAT CAAGAAGACG 
AAGCAGCTGC GGAGCGAGGT CTACGACCCA TCCGACCCCA CCGGCTCCGA CTCCAGCGCC 
CCTGGCAGCA GCCCCGAGAG GTCTGGCCCC GGCCTCCTGC CCTCTGAGAT CACACGAACC 
ATCTCCATCA ACAGCCCGAA GGCCCAGACG GTGCAGGCTG TGCGCTGCGT CACCTCCTAC 
ACGGTGGAGA GCATCTTTGG TACAGAGCCC GAACCCCCTC TCGGACCGTC CTCCGCCATG 
TCCAAGCTCC GGGGTGCAGT GGCTGCCGAG GGGGCCTCTG ACACGGAGCG AGAGGAGCCC 
ACAGAGAGCC AGGGCCTGGC TGCCCGGCTG CGGAGGCCAT CCCCCCCAGA GCCCTGGGAT 
GAGGAGGATG GGGCGTCTTG CAGCACCTTC TTTGGCTCTG AGGAGCGGAC GGTGACCTGT 
GTGACTGTCG TGGAGCCGGA AGCCCCACCC AGCCCGGACG TGCTGCAGGC TGCCACCCAC 
AGAGTCGTGG AGCTCAGGCC CCCTTCCCGG TCCCGCTCCA CATCCAGCTC CCGCAGCAGG 
AAGAAGGCCA AGAGGAAGAG GGTGTCCAGG GAGCACGGAC GGACGCGCTC TGGGACGCGC 
TCTGAATCCA GGGACAGGAG CTCGAGGTCA GCGTCACCAT CAGTGGGTGA GGAGCGCCCC 
AGGAGGCAGC GGTCCAAGGC CAAGAGCCGG CGGTCCTCCA GTGACCGCTC CAGCAGCCGA 
GAGCGAGCTA AGAGGAAGAA AGCCAAGGAC AAGAGCAGGG AGCACAGGCG GGGCCCCTGG 
GGCCACAGCC GGAGGACGTC CCGGTCGCGG TCGGGGAGCC CTGGCAGCTC TTCCTATGAG 
CACTATGAGA GTAGGAAGAA GAAGAAAAGG AGATCAGCGT CCAGACCTCG GGGAAGGGAG 
TGCTCCCCCA CCAGCAGCCT GGAGAGGCTC TGCAGGCACA AGCATCAGCG GGAACGCAGC 
CACGAGCGGC CAGACAGGAA GGAGAGTGTG GCGTGGCCCC GAGACCGGAG GAAGCGGAGG 
TCCCGGTCCC CAAGCTCGGA GCACAGGGCA CGGGAGCACA GGCGGCCTCG GTCCCGTGAG 
AAGTGGCCGC AGACCCGGTC CCATTCCCCA GAGAGGAAGG GGGCTGTGAG GGAGGCTTCC 
CCAGCGCCCC TTGCACAGGG GGAGCCAGGG CGGGAAGACC TCCCCACCAG GTTGCCAGCC 
TTGGGGGAAG CACATGTCTC GCCGGAGGTG GCTACGGCCG ACAAGGCCCC CCTGCAGGCT 
CCCCCTGTCC TGGAGGTGGC AGCTGAGTGT GAGCCGGACG ACCTGGACCT GGATTATGGC 
GACTCCGTGG AGGCCGGACA CGTCTTTGAT GATTTCTCAA GCGACGCCGT TTTCATCCAG 
CTCGATGACA TGAGCTCGCC ACCTTCTCCC GAAAGCACAG ACTCTTCCCC GGAGCGAGAC 
TTCCCACTGA AGCCTGCGTT GCCCCCAGCC AGCCTGGCCG TGGCCGCCAT CCAGAGGGAG 
GTGTCATTGA TGCACGATGA AGACCCTTCG CAGCCCCCAC CCCTGCCAGA GGGCACCCAG 
GAGCCACATT TGCTCAGGCC GGACGCGGCT GAGAAGGCTG AGGCACCCAG TTCCCCGGAT 
GTGGCGCCTG CGGGGAAGGA AGACAGCCCC TCTGCGAGTG GGAGGGTACA GGAGGCAGCC 
CGGCCTGAGG AGGTGGTTTC GCAGACCCCC CTGCTGCGGT CCAGAGCCCT GGTGAAGCGG 
GTCACCTGGA ACCTGCAGGA GTCGGAGAGC AGCGCCCCCG CCGAGGACAG AGCCCCCCGG 
GCACCACTTC ACAGGCCACA GAAGCCCCGA GAAGGAGCCT GGGACATGGA GGATGTGGCC 
CCCACAGGGG TCAGGCAGGT GTTCTCCGAG CTGCCCTTTC CCAGTCACGT GCTTCCGGAA 
CCCGGGTTCC CAGACACAGA CCCCTCTCAG GTTTACAGCC CCGGCCTGCC GCCTGCCCCG 
GCCCAGCCCT CAAGCATCCC ACCCTGCGCA CTGGTCAGCC AGCCCACGGT CCAGTTCATC 
CTTCAGGGGA GCCTGCCGCT AGTGGGCTGT GGGGCAGCAC AGACCCTGGC CCCAGTGCCC 
GCTGCCCTGA CCCCAGCCTC AGAGCCAGCC AGTCAAGCCA CTGCAGCCAG CAACTCGGAG 
GAGAAGACCC CGGCCCCCAG GCTAGCTGCG GAGAAAACCA AGAAGGAGGA GTACATGAAG 
AAGCTGCACA TGCAGGAGCG TGCTGTGGAG GAGGTGAAGC TGGCCATCAA GCCCTTCTAC 
CAGAAGAGGG AGGTGACCAA GGAGGAGTAC AAGGACATCC TGCGCAAGGC CGTGCAGAAG 
ATCTGCCACA GCAAGAGTGG AGAGATCAAC CCCGTGAAGG TGGCCAACCT GGTGAAGGCG 
TACGTGGACA AGTACAGGCA CATGCGCAGG CACAAGAAAC CAGAGGCCGG GGAGGAGCCG 
CCCACGCAGG GGGCCGAGGG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_011520237.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518539.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu70811
Clone ID Related Accession (Same CDS sequence)	XM_011520237.3 , XM_011520237.2
Accession Version	XM_011520237.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011520237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518539.1
CDS	130..4959
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_011520237.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520237.3

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHRF1 ( XM_011520237.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518539.1 Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu70811
Clone ID Related Accession (Same CDS sequence)	XM_011520237.3 , XM_011520237.2
Accession Version	XM_011520237.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518539.1
CDS	130..4959
Translation	MDDDSLDELVARSPGPDGHPQVGPADPAGDFGSEDSEDDGETLLEVAGTQGKLEAAGSFNSDDDAESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKILRKIPVENTKASEEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECAAPGVVLAADAGPVSEEEVSLLLADVVPTTSRLRPRAGRTRAIARTRQSERVRATVNRNRISTARRVQHTPGRLGSSLLDEAIEAVATGLSTAVYQRPLTPRTPARRKRKTRRRKKVPGRKKTPSGPSAKSKSSATRSKKRQHRVKKRRGKKVKSEATTRSRIARTLGLRRPVHSSCIPSVLKPVEPSLGLLRADIGAASLSLFGDPYELDPFDSSEELSANPLSPLSAKRRALSRSALQSHQPVARPVSVGLSRRRLPAAVPEPDLEEEPVPDLLGSILSGQSLLMLGSSDVIIHRDGSLSAKRAAPVSFQRNSGSLSRGEEGFKGCLQPRALPSGSPAQGPSGNRPQSTGLSCQGRSRTPARTAGAPVRLDLPAAPGAVQARNLSNGSVPGFRQSHSPWFNGTNKHTLPLASAASKISSRDSKPPCRSVVPGPPLKPAPRRTDISELPRIPKIRRDDGGGRRDAAPAHGQSIEIPSACISRLTGREGTGQPGRGTRAESEASSRVPREPGVHTGSSRPPAPSSHGSLAPLGPSRGKGVGSTFESFRINIPGNMAHSSQLSSPGFCNTFRPVDDKEQRKENPSPLFSIKKTKQLRSEVYDPSDPTGSDSSAPGSSPERSGPGLLPSEITRTISINSPKAQTVQAVRCVTSYTVESIFGTEPEPPLGPSSAMSKLRGAVAAEGASDTEREEPTESQGLAARLRRPSPPEPWDEEDGASCSTFFGSEERTVTCVTVVEPEAPPSPDVLQAATHRVVELRPPSRSRSTSSSRSRKKAKRKRVSREHGRTRSGTRSESRDRSSRSASPSVGEERPRRQRSKAKSRRSSSDRSSSRERAKRKKAKDKSREHRRGPWGHSRRTSRSRSGSPGSSSYEHYESRKKKKRRSASRPRGRECSPTSSLERLCRHKHQRERSHERPDRKESVAWPRDRRKRRSRSPSSEHRAREHRRPRSREKWPQTRSHSPERKGAVREASPAPLAQGEPGREDLPTRLPALGEAHVSPEVATADKAPLQAPPVLEVAAECEPDDLDLDYGDSVEAGHVFDDFSSDAVFIQLDDMSSPPSPESTDSSPERDFPLKPALPPASLAVAAIQREVSLMHDEDPSQPPPLPEGTQEPHLLRPDAAEKAEAPSSPDVAPAGKEDSPSASGRVQEAARPEEVVSQTPLLRSRALVKRVTWNLQESESSAPAEDRAPRAPLHRPQKPREGAWDMEDVAPTGVRQVFSELPFPSHVLPEPGFPDTDPSQVYSPGLPPAPAQPSSIPPCALVSQPTVQFILQGSLPLVGCGAAQTLAPVPAALTPASEPASQATAASNSEEKTPAPRLAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKPEAGEEPPTQGAEG

Target ORF information:

RefSeq Version	XM_011520237.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PHD and ring finger domains 1 (PHRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520237.2

ORF Insert Sequence:

ATGGATGACG ACAGCCTGGA TGAGCTTGTG GCCCGGAGCC CAGGGCCGGA TGGACACCCA 
CAGGTCGGCC CTGCGGACCC GGCAGGTGAC TTTGGTTCCG AGGATTCTGA AGACGACGGG 
GAGACATTGC TGGAGGTAGC GGGTACTCAG GGGAAACTGG AAGCCGCTGG CTCTTTCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCAATC TGTCTCAACG CATTCAGAGA CCAGGCCGTG 
GGGACGCCGG AGAACTGTGC CCATTACTTC TGCCTGGACT GCATTGTCGA ATGGTCCAAG 
AATGCCAATT CCTGTCCAGT TGATCGAACT CTATTTAAGT GCATTTGTAT TCGAGCTCAA 
TTTGGTGGTA AAATCTTAAG AAAGATCCCA GTGGAGAACA CCAAAGCGAG CGAGGAGGAG 
GAGGACCCGA CCTTCTGTGA GGTGTGCGGC AGGAGCGACC GTGAGGACAG GCTTTTGCTC 
TGCGACGGCT GCGATGCGGG GTACCACATG GAATGCTTGG ACCCCCCTCT CCAGGAGGTG 
CCGGTGGACG AGTGGTTCTG CCCGGAATGT GCTGCGCCTG GTGTTGTCCT TGCCGCTGAT 
GCGGGTCCCG TGAGTGAGGA GGAGGTCTCC CTGCTCTTGG CTGATGTGGT GCCCACCACC 
AGCAGGCTTC GGCCTCGAGC AGGTAGGACC CGGGCGATAG CCAGGACACG GCAGAGTGAG 
AGAGTGAGAG CAACCGTGAA CCGGAACCGG ATCTCCACGG CCAGGAGGGT CCAGCACACA 
CCAGGGCGCC TCGGGTCTTC CCTGCTGGAT GAAGCCATCG AGGCTGTGGC GACTGGCCTG 
AGCACTGCCG TGTATCAGCG CCCCCTGACG CCGCGCACTC CCGCCCGACG GAAGAGGAAG 
ACAAGAAGAC GGAAGAAAGT GCCGGGAAGA AAGAAAACCC CGTCCGGACC ATCCGCAAAA 
AGTAAGAGCT CAGCGACAAG ATCTAAGAAA CGCCAACATC GAGTGAAGAA GAGAAGAGGG 
AAGAAGGTAA AGAGTGAAGC CACCACTCGC TCTCGAATCG CGCGGACGCT GGGCCTGCGC 
AGGCCTGTTC ACAGCAGCTG CATCCCGTCA GTGTTGAAGC CAGTGGAGCC CTCTTTGGGG 
CTGCTGAGAG CGGATATTGG AGCTGCCTCT CTGTCTCTGT TTGGAGATCC TTATGAGCTG 
GATCCCTTCG ACAGCAGTGA AGAGCTTTCT GCAAACCCTC TTTCCCCTCT GAGTGCCAAG 
AGACGGGCTC TGTCCCGGTC AGCCCTGCAG TCCCACCAGC CCGTGGCCAG GCCCGTCTCC 
GTGGGGCTTT CCAGGAGGCG CCTCCCTGCC GCGGTGCCAG AGCCAGACTT GGAGGAGGAG 
CCAGTGCCTG ACCTGCTGGG CAGCATCCTG TCGGGCCAGA GCCTCCTGAT GCTGGGCAGC 
AGTGATGTCA TCATCCACCG CGACGGCTCC CTCAGCGCCA AGAGGGCGGC TCCAGTTTCT 
TTTCAGCGAA ACTCAGGCAG TCTGTCCAGA GGGGAAGAAG GATTCAAGGG CTGCCTGCAG 
CCCCGAGCAC TGCCCTCCGG GAGCCCGGCC CAAGGCCCGT CAGGAAACAG GCCACAGAGC 
ACAGGGCTCA GCTGTCAAGG CAGGTCCCGC ACCCCCGCCC GCACCGCGGG GGCGCCTGTG 
AGGCTGGACT TGCCAGCAGC CCCTGGGGCG GTTCAGGCTC GGAACTTGTC AAATGGGAGT 
GTGCCTGGCT TCAGACAGAG CCACAGCCCC TGGTTCAACG GCACCAACAA GCACACCTTG 
CCCCTTGCCT CTGCCGCGTC TAAGATCTCA AGCAGAGATT CTAAGCCCCC ATGTCGCAGT 
GTGGTGCCGG GGCCTCCCCT GAAGCCAGCG CCCAGAAGAA CAGACATCTC TGAGCTACCC 
AGGATACCAA AGATCAGGAG AGATGACGGT GGTGGCAGAC GGGATGCGGC CCCGGCCCAC 
GGGCAGAGCA TTGAGATCCC CAGTGCCTGC ATCAGCCGAC TGACTGGCAG GGAGGGCACC 
GGGCAGCCAG GGCGAGGCAC ACGGGCAGAG AGCGAGGCCA GCAGCAGGGT GCCCCGGGAG 
CCCGGGGTGC ACACGGGCAG CTCCCGGCCC CCAGCCCCCA GCTCCCATGG CAGTTTGGCC 
CCACTGGGAC CATCAAGAGG GAAAGGGGTC GGGTCGACCT TTGAGAGCTT CCGGATCAAT 
ATTCCTGGAA ACATGGCACA TTCCAGCCAG CTCTCCAGCC CTGGCTTCTG TAACACGTTC 
CGGCCTGTGG ACGATAAGGA GCAGAGGAAG GAGAACCCCT CACCCCTCTT CTCCATCAAG 
AAGACGAAGC AGCTGCGGAG CGAGGTCTAC GACCCATCCG ACCCCACCGG CTCCGACTCC 
AGCGCCCCTG GCAGCAGCCC CGAGAGGTCT GGCCCCGGCC TCCTGCCCTC TGAGATCACA 
CGAACCATCT CCATCAACAG CCCGAAGGCC CAGACGGTGC AGGCTGTGCG CTGCGTCACC 
TCCTACACGG TGGAGAGCAT CTTTGGTACA GAGCCCGAAC CCCCTCTCGG ACCGTCCTCC 
GCCATGTCCA AGCTCCGGGG TGCAGTGGCT GCCGAGGGGG CCTCTGACAC GGAGCGAGAG 
GAGCCCACAG AGAGCCAGGG CCTGGCTGCC CGGCTGCGGA GGCCATCCCC CCCAGAGCCC 
TGGGATGAGG AGGATGGGGC GTCTTGCAGC ACCTTCTTTG GCTCTGAGGA GCGGACGGTG 
ACCTGTGTGA CTGTCGTGGA GCCGGAAGCC CCACCCAGCC CGGACGTGCT GCAGGCTGCC 
ACCCACAGAG TCGTGGAGCT CAGGCCCCCT TCCCGGTCCC GCTCCACATC CAGCTCCCGC 
AGCAGGAAGA AGGCCAAGAG GAAGAGGGTG TCCAGGGAGC ACGGACGGAC GCGCTCTGGG 
ACGCGCTCTG AATCCAGGGA CAGGAGCTCG AGGTCAGCGT CACCATCAGT GGGTGAGGAG 
CGCCCCAGGA GGCAGCGGTC CAAGGCCAAG AGCCGGCGGT CCTCCAGTGA CCGCTCCAGC 
AGCCGAGAGC GAGCTAAGAG GAAGAAAGCC AAGGACAAGA GCAGGGAGCA CAGGCGGGGC 
CCCTGGGGCC ACAGCCGGAG GACGTCCCGG TCGCGGTCGG GGAGCCCTGG CAGCTCTTCC 
TATGAGCACT ATGAGAGTAG GAAGAAGAAG AAAAGGAGAT CAGCGTCCAG ACCTCGGGGA 
AGGGAGTGCT CCCCCACCAG CAGCCTGGAG AGGCTCTGCA GGCACAAGCA TCAGCGGGAA 
CGCAGCCACG AGCGGCCAGA CAGGAAGGAG AGTGTGGCGT GGCCCCGAGA CCGGAGGAAG 
CGGAGGTCCC GGTCCCCAAG CTCGGAGCAC AGGGCACGGG AGCACAGGCG GCCTCGGTCC 
CGTGAGAAGT GGCCGCAGAC CCGGTCCCAT TCCCCAGAGA GGAAGGGGGC TGTGAGGGAG 
GCTTCCCCAG CGCCCCTTGC ACAGGGGGAG CCAGGGCGGG AAGACCTCCC CACCAGGTTG 
CCAGCCTTGG GGGAAGCACA TGTCTCGCCG GAGGTGGCTA CGGCCGACAA GGCCCCCCTG 
CAGGCTCCCC CTGTCCTGGA GGTGGCAGCT GAGTGTGAGC CGGACGACCT GGACCTGGAT 
TATGGCGACT CCGTGGAGGC CGGACACGTC TTTGATGATT TCTCAAGCGA CGCCGTTTTC 
ATCCAGCTCG ATGACATGAG CTCGCCACCT TCTCCCGAAA GCACAGACTC TTCCCCGGAG 
CGAGACTTCC CACTGAAGCC TGCGTTGCCC CCAGCCAGCC TGGCCGTGGC CGCCATCCAG 
AGGGAGGTGT CATTGATGCA CGATGAAGAC CCTTCGCAGC CCCCACCCCT GCCAGAGGGC 
ACCCAGGAGC CACATTTGCT CAGGCCGGAC GCGGCTGAGA AGGCTGAGGC ACCCAGTTCC 
CCGGATGTGG CGCCTGCGGG GAAGGAAGAC AGCCCCTCTG CGAGTGGGAG GGTACAGGAG 
GCAGCCCGGC CTGAGGAGGT GGTTTCGCAG ACCCCCCTGC TGCGGTCCAG AGCCCTGGTG 
AAGCGGGTCA CCTGGAACCT GCAGGAGTCG GAGAGCAGCG CCCCCGCCGA GGACAGAGCC 
CCCCGGGCAC CACTTCACAG GCCACAGAAG CCCCGAGAAG GAGCCTGGGA CATGGAGGAT 
GTGGCCCCCA CAGGGGTCAG GCAGGTGTTC TCCGAGCTGC CCTTTCCCAG TCACGTGCTT 
CCGGAACCCG GGTTCCCAGA CACAGACCCC TCTCAGGTTT ACAGCCCCGG CCTGCCGCCT 
GCCCCGGCCC AGCCCTCAAG CATCCCACCC TGCGCACTGG TCAGCCAGCC CACGGTCCAG 
TTCATCCTTC AGGGGAGCCT GCCGCTAGTG GGCTGTGGGG CAGCACAGAC CCTGGCCCCA 
GTGCCCGCTG CCCTGACCCC AGCCTCAGAG CCAGCCAGTC AAGCCACTGC AGCCAGCAAC 
TCGGAGGAGA AGACCCCGGC CCCCAGGCTA GCTGCGGAGA AAACCAAGAA GGAGGAGTAC 
ATGAAGAAGC TGCACATGCA GGAGCGTGCT GTGGAGGAGG TGAAGCTGGC CATCAAGCCC 
TTCTACCAGA AGAGGGAGGT GACCAAGGAG GAGTACAAGG ACATCCTGCG CAAGGCCGTG 
CAGAAGATCT GCCACAGCAA GAGTGGAGAG ATCAACCCCG TGAAGGTGGC CAACCTGGTG 
AAGGCGTACG TGGACAAGTA CAGGCACATG CGCAGGCACA AGAAACCAGA GGCCGGGGAG 
GAGCCGCCCA CGCAGGGGGC CGAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHRF1
Entrez Gene ID	57661
Full Name	PHD and ring finger domains 1
Synonyms	PPP1R125,RNF221
General protein information	Preferred Names PHD and ring finger domains 1 Names PHD and RING finger domain-containing protein 1 CTD-binding SR-like protein rA9 protein phosphatase 1, regulatory subunit 125
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Disorder MIM:	611780

Pan troglodytes (chimpanzee)	PHRF1	XP_508199.3
Mus musculus (house mouse)	Phrf1	NP_001074587.1
Homo sapiens (human)	PHRF1	NP_001273510.1
	PHRF1	XP_001086134.2
Canis lupus familiaris (dog)	PHRF1	XP_540525.3
Rattus norvegicus (Norway rat)	Phrf1	NP_620793.1
Gallus gallus (chicken)	PHRF1	XP_004941520.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHRF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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