Phrf1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006536141.3	XP_006536204.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536144.3	XP_006536207.1	PHD and RING finger domain-containing protein 1 isoform X7
XM_006536139.4	XP_006536202.1	PHD and RING finger domain-containing protein 1 isoform X3
XM_006536145.4	XP_006536208.1	PHD and RING finger domain-containing protein 1 isoform X8
XM_006536145.3	XP_006536208.1	PHD and RING finger domain-containing protein 1 isoform X8
XM_006536144.4	XP_006536207.1	PHD and RING finger domain-containing protein 1 isoform X7
NM_001081118.1	NP_001074587.1	PHD and RING finger domain-containing protein 1
XM_006536138.3	XP_006536201.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_011249837.2	XP_011248139.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536137.3	XP_006536200.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_006536142.1	XP_006536205.1	PHD and RING finger domain-containing protein 1 isoform X5
XM_006536143.3	XP_006536206.1	PHD and RING finger domain-containing protein 1 isoform X6
XM_006536137.4	XP_006536200.1	PHD and RING finger domain-containing protein 1 isoform X1
XM_011249837.3	XP_011248139.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536142.2	XP_006536205.1	PHD and RING finger domain-containing protein 1 isoform X5
XM_030241933.1	XP_030097793.1	PHD and RING finger domain-containing protein 1 isoform X5
XM_006536139.3	XP_006536202.1	PHD and RING finger domain-containing protein 1 isoform X3
XM_006536140.1	XP_006536203.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536138.4	XP_006536201.1	PHD and RING finger domain-containing protein 1 isoform X2
XM_006536140.2	XP_006536203.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536141.4	XP_006536204.1	PHD and RING finger domain-containing protein 1 isoform X4
XM_006536143.4	XP_006536206.1	PHD and RING finger domain-containing protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40025	XM_006536137.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40026	XM_006536138.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40027	XM_006536139.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40028	XM_011249837.3 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40028	XM_006536141.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40028	XM_006536140.2 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40029	XM_006536142.2 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40029	XM_030241933.1 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40030	XM_006536143.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40031	XM_006536144.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu40032	XM_006536145.4 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu01728	NM_001081118.1 Latest version!	Mus musculus PHD and ring finger domains 1 (Phrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu40032	XM_006536145.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40031	XM_006536144.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40025	XM_006536137.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40028	XM_011249837.2	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40029	XM_006536142.1	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40027	XM_006536139.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40026	XM_006536138.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40028	XM_006536140.1	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40028	XM_006536141.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu40030	XM_006536143.3	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

Phrf1 ( XM_006536137.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536200.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40025
Clone ID Related Accession (Same CDS sequence)	XM_006536137.3 , XM_006536137.4
Accession Version	XM_006536137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536137.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGCCCCC TGGTGCTCCC 
AGGGCAGAAG GGCTTCCAGC TGCTGGGACT CTGCACTCAG CAGGGGGTAT TCTTGCCCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536200.1
CDS	39..5186
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536137.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536137.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGCCCCC TGGTGCTCCC 
AGGGCAGAAG GGCTTCCAGC TGCTGGGACT CTGCACTCAG CAGGGGGTAT TCTTGCCCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536138.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536201.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

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CloneID	OMu40026
Clone ID Related Accession (Same CDS sequence)	XM_006536138.3 , XM_006536138.4
Accession Version	XM_006536138.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536138.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGTTGACC ACAAGGCATC CACACTCCCT GTCTGTTGGG 
TCCTCAGGGA CACCACTTCA GAGGCCCCAG AGGCCCCAGG AAGGAGACTG GGATGCAGAG 
GACAGGGCCC TCATAGGATT TCAGCAGGCA CCATTCTCCG AGCTGCCCCC TCCCATCCAC 
GTGCTCCAGG AGTCTGGGTT ACCTGATGCA GATCCCTCTC AGCCCCCTGG TGCTCCCAGG 
GCAGAAGGGC TTCCAGCTGC TGGGACTCTG CACTCAGCAG GGGGTATTCT TGCCCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536201.1
CDS	39..5183
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536138.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536138.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGTTGACC ACAAGGCATC CACACTCCCT GTCTGTTGGG 
TCCTCAGGGA CACCACTTCA GAGGCCCCAG AGGCCCCAGG AAGGAGACTG GGATGCAGAG 
GACAGGGCCC TCATAGGATT TCAGCAGGCA CCATTCTCCG AGCTGCCCCC TCCCATCCAC 
GTGCTCCAGG AGTCTGGGTT ACCTGATGCA GATCCCTCTC AGCCCCCTGG TGCTCCCAGG 
GCAGAAGGGC TTCCAGCTGC TGGGACTCTG CACTCAGCAG GGGGTATTCT TGCCCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536139.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536202.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu40027
Clone ID Related Accession (Same CDS sequence)	XM_006536139.4 , XM_006536139.3
Accession Version	XM_006536139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
CCCCCTGGTG CTCCCAGGGC AGAAGGGCTT CCAGCTGCTG GGACTCTGCA CTCAGCAGGG 
GGTATTCTTG CCCAGGTTTA CAGCCCCAAC ATGCCACCAC CCTTGGCTCA GCCCTCAAGC 
ATCCTGCCCT ATGCACTGGT CAGCCAGCCC TCAGTCCAGT TGATCCTGCA GGGGACCCTT 
CCCCTAGCAG GCTGCGGTAC AGCACAGAGC CTGGCACCAG TGCCCACAAT GCCAGCTACA 
GTCTCAGAGC TAGCTGTTCC AACCACCAAC AACTCAGAAG AAAGGACTGC TACTCCCAAA 
ACAGCTGCTG AGAAGACCAA AAAGGAGGAG TACATGAAGA AGCTGCACAT GCAAGAACGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAA CCCTTCTACC AGAAGAGAGA AGTGACCAAG 
GAGGAGTACA AGGACATACT GCGCAAAGCC GTGCAGAAGA TCTGCCACAG CAAGAGTGGT 
GAAATCAACC CTGTAAAGGT GGCCAACCTG GTGAAGGCCT ATGTGGACAA ATACAGGCAT 
ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA GAGGAGCCAC CCACTCAGGG TGCTGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536202.1
CDS	38..5140
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536139.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536139.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
CCCCCTGGTG CTCCCAGGGC AGAAGGGCTT CCAGCTGCTG GGACTCTGCA CTCAGCAGGG 
GGTATTCTTG CCCAGGTTTA CAGCCCCAAC ATGCCACCAC CCTTGGCTCA GCCCTCAAGC 
ATCCTGCCCT ATGCACTGGT CAGCCAGCCC TCAGTCCAGT TGATCCTGCA GGGGACCCTT 
CCCCTAGCAG GCTGCGGTAC AGCACAGAGC CTGGCACCAG TGCCCACAAT GCCAGCTACA 
GTCTCAGAGC TAGCTGTTCC AACCACCAAC AACTCAGAAG AAAGGACTGC TACTCCCAAA 
ACAGCTGCTG AGAAGACCAA AAAGGAGGAG TACATGAAGA AGCTGCACAT GCAAGAACGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAA CCCTTCTACC AGAAGAGAGA AGTGACCAAG 
GAGGAGTACA AGGACATACT GCGCAAAGCC GTGCAGAAGA TCTGCCACAG CAAGAGTGGT 
GAAATCAACC CTGTAAAGGT GGCCAACCTG GTGAAGGCCT ATGTGGACAA ATACAGGCAT 
ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA GAGGAGCCAC CCACTCAGGG TGCTGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_011249837.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248139.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.

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CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_011249837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248139.1
CDS	330..5423
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_011249837.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249837.3

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536140.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536203.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.

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CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_006536140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536203.1
CDS	147..5240
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536140.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536140.2

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536141.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536204.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.

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CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_006536141.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536141.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536204.1
CDS	656..5749
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536141.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536141.4

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536142.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536205.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.

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CloneID	OMu40029
Clone ID Related Accession (Same CDS sequence)	XM_006536142.1 , XM_006536142.2 , XM_030241933.1
Accession Version	XM_006536142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536142.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536205.1
CDS	155..5245
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536142.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536142.2

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_030241933.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030097793.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.

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CloneID	OMu40029
Clone ID Related Accession (Same CDS sequence)	XM_006536142.1 , XM_006536142.2 , XM_030241933.1
Accession Version	XM_030241933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030097793.1
CDS	389..5479
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_030241933.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030241933.1

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536143.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536206.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu40030
Clone ID Related Accession (Same CDS sequence)	XM_006536143.3 , XM_006536143.4
Accession Version	XM_006536143.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536143.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGGTTTA CAGCCCCAAC 
ATGCCACCAC CCTTGGCTCA GCCCTCAAGC ATCCTGCCCT ATGCACTGGT CAGCCAGCCC 
TCAGTCCAGT TGATCCTGCA GGGGACCCTT CCCCTAGCAG GCTGCGGTAC AGCACAGAGC 
CTGGCACCAG TGCCCACAAT GCCAGCTACA GTCTCAGAGC TAGCTGTTCC AACCACCAAC 
AACTCAGAAG AAAGGACTGC TACTCCCAAA ACAGCTGCTG AGAAGACCAA AAAGGAGGAG 
TACATGAAGA AGCTGCACAT GCAAGAACGG GCTGTGGAGG AGGTGAAGCT GGCCATTAAA 
CCCTTCTACC AGAAGAGAGA AGTGACCAAG GAGGAGTACA AGGACATACT GCGCAAAGCC 
GTGCAGAAGA TCTGCCACAG CAAGAGTGGT GAAATCAACC CTGTAAAGGT GGCCAACCTG 
GTGAAGGCCT ATGTGGACAA ATACAGGCAT ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA 
GAGGAGCCAC CCACTCAGGG TGCTGAGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536206.1
CDS	41..5113
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536143.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536143.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGGTTTA CAGCCCCAAC 
ATGCCACCAC CCTTGGCTCA GCCCTCAAGC ATCCTGCCCT ATGCACTGGT CAGCCAGCCC 
TCAGTCCAGT TGATCCTGCA GGGGACCCTT CCCCTAGCAG GCTGCGGTAC AGCACAGAGC 
CTGGCACCAG TGCCCACAAT GCCAGCTACA GTCTCAGAGC TAGCTGTTCC AACCACCAAC 
AACTCAGAAG AAAGGACTGC TACTCCCAAA ACAGCTGCTG AGAAGACCAA AAAGGAGGAG 
TACATGAAGA AGCTGCACAT GCAAGAACGG GCTGTGGAGG AGGTGAAGCT GGCCATTAAA 
CCCTTCTACC AGAAGAGAGA AGTGACCAAG GAGGAGTACA AGGACATACT GCGCAAAGCC 
GTGCAGAAGA TCTGCCACAG CAAGAGTGGT GAAATCAACC CTGTAAAGGT GGCCAACCTG 
GTGAAGGCCT ATGTGGACAA ATACAGGCAT ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA 
GAGGAGCCAC CCACTCAGGG TGCTGAGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536144.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536207.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu40031
Clone ID Related Accession (Same CDS sequence)	XM_006536144.3 , XM_006536144.4
Accession Version	XM_006536144.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536207.1
CDS	40..5067
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536144.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536144.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536145.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536208.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X11, mRNA.

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CloneID	OMu40032
Clone ID Related Accession (Same CDS sequence)	XM_006536145.4 , XM_006536145.3
Accession Version	XM_006536145.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006536145.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGACACCA CTTCAGAGGC CCCAGAGGCC CCAGGAAGGA 
GACTGGGATG CAGAGGACAG GGCCCTCATA GGATTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGATCC CTCTCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536208.1
CDS	41..5065
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536145.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536145.4

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGACACCA CTTCAGAGGC CCCAGAGGCC CCAGGAAGGA 
GACTGGGATG CAGAGGACAG GGCCCTCATA GGATTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGATCC CTCTCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( NM_001081118.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074587.1 Mus musculus PHD and ring finger domains 1 (Phrf1), mRNA.

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CloneID	OMu01728
Clone ID Related Accession (Same CDS sequence)	NM_001081118.1
Accession Version	NM_001081118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC108908.16 and AC163434.5. On Feb 6, 2007 this sequence version replaced XM_133935.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGACACCAC TTCAGAGGCC CCAGAGGCCC 
CAGGAAGGAG ACTGGGATGC AGAGGACAGG GCCCTCATAG GATTTCAGCA GGCACCATTC 
TCCGAGCTGC CCCCTCCCAT CCACGTGCTC CAGGAGTCTG GGTTACCTGA TGCAGATCCC 
TCTCAGCCCC CTGGTGCTCC CAGGGCAGAA GGGCTTCCAG CTGCTGGGAC TCTGCACTCA 
GCAGGGGGTA TTCTTGCCCA GGTTTACAGC CCCAACATGC CACCACCCTT GGCTCAGCCC 
TCAAGCATCC TGCCCTATGC ACTGGTCAGC CAGCCCTCAG TCCAGTTGAT CCTGCAGGGG 
ACCCTTCCCC TAGCAGGCTG CGGTACAGCA CAGAGCCTGG CACCAGTGCC CACAATGCCA 
GCTACAGTCT CAGAGCTAGC TGTTCCAACC ACCAACAACT CAGAAGAAAG GACTGCTACT 
CCCAAAACAG CTGCTGAGAA GACCAAAAAG GAGGAGTACA TGAAGAAGCT GCACATGCAA 
GAACGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAACCCT TCTACCAGAA GAGAGAAGTG 
ACCAAGGAGG AGTACAAGGA CATACTGCGC AAAGCCGTGC AGAAGATCTG CCACAGCAAG 
AGTGGTGAAA TCAACCCTGT AAAGGTGGCC AACCTGGTGA AGGCCTATGT GGACAAATAC 
AGGCATATGC GCAGACACAA GAAGACTGAA GGTGGAGAGG AGCCACCCAC TCAGGGTGCT 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074587.1
CDS	127..5175
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	NM_001081118.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081118.1

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGACACCAC TTCAGAGGCC CCAGAGGCCC 
CAGGAAGGAG ACTGGGATGC AGAGGACAGG GCCCTCATAG GATTTCAGCA GGCACCATTC 
TCCGAGCTGC CCCCTCCCAT CCACGTGCTC CAGGAGTCTG GGTTACCTGA TGCAGATCCC 
TCTCAGCCCC CTGGTGCTCC CAGGGCAGAA GGGCTTCCAG CTGCTGGGAC TCTGCACTCA 
GCAGGGGGTA TTCTTGCCCA GGTTTACAGC CCCAACATGC CACCACCCTT GGCTCAGCCC 
TCAAGCATCC TGCCCTATGC ACTGGTCAGC CAGCCCTCAG TCCAGTTGAT CCTGCAGGGG 
ACCCTTCCCC TAGCAGGCTG CGGTACAGCA CAGAGCCTGG CACCAGTGCC CACAATGCCA 
GCTACAGTCT CAGAGCTAGC TGTTCCAACC ACCAACAACT CAGAAGAAAG GACTGCTACT 
CCCAAAACAG CTGCTGAGAA GACCAAAAAG GAGGAGTACA TGAAGAAGCT GCACATGCAA 
GAACGGGCTG TGGAGGAGGT GAAGCTGGCC ATTAAACCCT TCTACCAGAA GAGAGAAGTG 
ACCAAGGAGG AGTACAAGGA CATACTGCGC AAAGCCGTGC AGAAGATCTG CCACAGCAAG 
AGTGGTGAAA TCAACCCTGT AAAGGTGGCC AACCTGGTGA AGGCCTATGT GGACAAATAC 
AGGCATATGC GCAGACACAA GAAGACTGAA GGTGGAGAGG AGCCACCCAC TCAGGGTGCT 
GAGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536137.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536200.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

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CloneID	OMu40025
Clone ID Related Accession (Same CDS sequence)	XM_006536137.3 , XM_006536137.4
Accession Version	XM_006536137.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGCCCCC TGGTGCTCCC 
AGGGCAGAAG GGCTTCCAGC TGCTGGGACT CTGCACTCAG CAGGGGGTAT TCTTGCCCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536200.1
CDS	253..5400
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536137.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536137.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGCCCCC TGGTGCTCCC 
AGGGCAGAAG GGCTTCCAGC TGCTGGGACT CTGCACTCAG CAGGGGGTAT TCTTGCCCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536138.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536201.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

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CloneID	OMu40026
Clone ID Related Accession (Same CDS sequence)	XM_006536138.3 , XM_006536138.4
Accession Version	XM_006536138.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGTTGACC ACAAGGCATC CACACTCCCT GTCTGTTGGG 
TCCTCAGGGA CACCACTTCA GAGGCCCCAG AGGCCCCAGG AAGGAGACTG GGATGCAGAG 
GACAGGGCCC TCATAGGATT TCAGCAGGCA CCATTCTCCG AGCTGCCCCC TCCCATCCAC 
GTGCTCCAGG AGTCTGGGTT ACCTGATGCA GATCCCTCTC AGCCCCCTGG TGCTCCCAGG 
GCAGAAGGGC TTCCAGCTGC TGGGACTCTG CACTCAGCAG GGGGTATTCT TGCCCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536201.1
CDS	254..5398
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536138.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536138.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGTTGACC ACAAGGCATC CACACTCCCT GTCTGTTGGG 
TCCTCAGGGA CACCACTTCA GAGGCCCCAG AGGCCCCAGG AAGGAGACTG GGATGCAGAG 
GACAGGGCCC TCATAGGATT TCAGCAGGCA CCATTCTCCG AGCTGCCCCC TCCCATCCAC 
GTGCTCCAGG AGTCTGGGTT ACCTGATGCA GATCCCTCTC AGCCCCCTGG TGCTCCCAGG 
GCAGAAGGGC TTCCAGCTGC TGGGACTCTG CACTCAGCAG GGGGTATTCT TGCCCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536139.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536202.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

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CloneID	OMu40027
Clone ID Related Accession (Same CDS sequence)	XM_006536139.4 , XM_006536139.3
Accession Version	XM_006536139.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
CCCCCTGGTG CTCCCAGGGC AGAAGGGCTT CCAGCTGCTG GGACTCTGCA CTCAGCAGGG 
GGTATTCTTG CCCAGGTTTA CAGCCCCAAC ATGCCACCAC CCTTGGCTCA GCCCTCAAGC 
ATCCTGCCCT ATGCACTGGT CAGCCAGCCC TCAGTCCAGT TGATCCTGCA GGGGACCCTT 
CCCCTAGCAG GCTGCGGTAC AGCACAGAGC CTGGCACCAG TGCCCACAAT GCCAGCTACA 
GTCTCAGAGC TAGCTGTTCC AACCACCAAC AACTCAGAAG AAAGGACTGC TACTCCCAAA 
ACAGCTGCTG AGAAGACCAA AAAGGAGGAG TACATGAAGA AGCTGCACAT GCAAGAACGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAA CCCTTCTACC AGAAGAGAGA AGTGACCAAG 
GAGGAGTACA AGGACATACT GCGCAAAGCC GTGCAGAAGA TCTGCCACAG CAAGAGTGGT 
GAAATCAACC CTGTAAAGGT GGCCAACCTG GTGAAGGCCT ATGTGGACAA ATACAGGCAT 
ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA GAGGAGCCAC CCACTCAGGG TGCTGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536202.1
CDS	253..5355
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536139.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536139.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
CCCCCTGGTG CTCCCAGGGC AGAAGGGCTT CCAGCTGCTG GGACTCTGCA CTCAGCAGGG 
GGTATTCTTG CCCAGGTTTA CAGCCCCAAC ATGCCACCAC CCTTGGCTCA GCCCTCAAGC 
ATCCTGCCCT ATGCACTGGT CAGCCAGCCC TCAGTCCAGT TGATCCTGCA GGGGACCCTT 
CCCCTAGCAG GCTGCGGTAC AGCACAGAGC CTGGCACCAG TGCCCACAAT GCCAGCTACA 
GTCTCAGAGC TAGCTGTTCC AACCACCAAC AACTCAGAAG AAAGGACTGC TACTCCCAAA 
ACAGCTGCTG AGAAGACCAA AAAGGAGGAG TACATGAAGA AGCTGCACAT GCAAGAACGG 
GCTGTGGAGG AGGTGAAGCT GGCCATTAAA CCCTTCTACC AGAAGAGAGA AGTGACCAAG 
GAGGAGTACA AGGACATACT GCGCAAAGCC GTGCAGAAGA TCTGCCACAG CAAGAGTGGT 
GAAATCAACC CTGTAAAGGT GGCCAACCTG GTGAAGGCCT ATGTGGACAA ATACAGGCAT 
ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA GAGGAGCCAC CCACTCAGGG TGCTGAGACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536141.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536204.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_006536141.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536204.1
CDS	272..5365
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536141.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536141.3

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_011249837.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248139.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_011249837.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248139.1
CDS	329..5422
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_011249837.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249837.2

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536140.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536203.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.

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CloneID	OMu40028
Clone ID Related Accession (Same CDS sequence)	XM_006536141.3 , XM_011249837.2 , XM_011249837.3 , XM_006536140.1 , XM_006536140.2 , XM_006536141.4
Accession Version	XM_006536140.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536203.1
CDS	348..5441
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536140.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536140.1

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAGAAA GCAGCAATGG CCAAAGTGGA 
GACTCTGAAG ATGATACAGG CAGTGAGCAG GATGATGACA CAGATGGGGA GGAAACAGAG 
GGGCTGTCTG AAGAGGAAGA CCCAGAGGAT AGATCTGGTT CTGAAGACTC AGAAGATGGA 
GTAGAAATGG CAACGGCAGC AATAGAGACT CAAGGGAAGC TTGAAGCTAG CAGTGTACCC 
AATTCTGATG ATGATGCAGA GAGCTGCCCC ATTTGCCTCA ATGCATTTAG AGACCAGGCT 
GTGGGCACCC CAGAGACCTG TGCCCATTAT TTCTGCCTGG ATTGCATCAT CGAATGGTCC 
AGGAATGCCA ACTCCTGTCC TGTTGATCGA ACAGTATTTA AATGTATTTG TATTCGTGCC 
CAGTTTAATG GTAAAATCTT AAAGAAGATT CCAGTAGAAA ACACTAAAGC TTGTGAGGCT 
GAGGAGGAAG ACCCGACTTT TTGTGAGGTG TGTGGCAGGA GTGATCGTGA GGACCGGCTC 
CTACTGTGTG ACGGCTGCGA TGCTGGGTAC CACATGGAAT GTTTGGACCC GCCTCTCCAG 
GAGGTGCCTG TGGATGAATG GTTCTGCCCA GAGTGTACGG TCCCTGGTGT GGATCCCACT 
CATGATGCAG CTCCTGTGAG TGATGAGGAG GTCTCCTTGC TACTGGCTGA TGTAGTACCT 
ACTACAAGCA GGCTTCGGCC TCGAGTAGGC AGGACCCGGG CCATCGCTAG GACACGGCAG 
AGTGAGAGAG TGAGGGCAAC TGTGAACCGG AACCGGATCT CCTCTGCCAG AAGGGTCCAG 
CATGTGCCAA GGTACCTCAT GTCTTCTCTG CTGGATGAAA CTATTGAGGC TGTCGCCACT 
GGGCTGAGCA CTGCTGTATA CCAGCGTCCC TTGACACCTC GTGTCCCTGC AAAACGGAAA 
AGGAAGGCAG GGAGACGAAA GAAAGTGCTG GGAAGAAAGA AAACTCGGTC CAGGTCATCT 
GTGAAAAGTA AGAGTGGGAG TACGAGAGCC AAGAAACGTC AGCACCGTGT AAGGAAGACA 
AAAGGGAGGA AGCTGAAGAA TGAAGTCACA GCTCGTTCTC GAATTGCACG AACACTAGGC 
CTCCGCAGGC CTGTCCGTGG CACCAGCATG CCATCAGTGT ACAAGCCAGT GGACCCCTCC 
CTCGGGCTGA TGAGGGCAGA CATTGGAGCA GCTTCCCTGT CACTGTTTGG AGATCCCTAT 
GAGCTGGATC CTTTTGACAG CAATGAAGAA CAGTCTGCAG ATCCACCTTC CCCTCTGAGT 
GCCAAGAGGA GAGTGCTGTC CCGCTCAGCT CTGCAGTCTC ACCAGCCTGT GGCCAGACCT 
GTCGCCATGG GGCTCTCTAG GAGGCAGCTC CCTGCTGTGG CCCCAGAGCC CAGCGTGGAG 
GAGGCCCCCG TTCCTGACCT GTTGGGAAGC ATCCTGTCTG GCCAGAGCCT CCTGATGATG 
AGCAGTGCTG ATGTTGTCAT ACACCGGGAT GGCTCTCTCA GCGCCAAGAG GGCAGCTCCT 
GTTTCTCTTC AGCGAAATTC TGTGACTCAA TCCAGAGAAG AGTCCAGGCT CAGAGACAAC 
CCGCAGCCTG GGGCACTACC CTCAGAGAGT GCATCTGGTG GATTCGTGGG AGACAGACAA 
CCAAACTCAG GGCTGAGCTG TGGGAATAGA ACAGCTCTGT GCTGTCTCCC TGCCCGCATT 
GCCCAGACAC CTGTGAGGTC AGATCCCTCA CTGACTCCTC GCTCAGGTCT GTCTAGGACC 
CTTTCAGATG AGAACAGGCC CAGCCGGACA CACAGCAGCA GCCCCCAACT CAATGGCTCC 
AATGTACGGG TTAGTTCTGC CTCGACTAAG ATCGTGACTC ATTCTAGCTT TCCATCGAAA 
AATACAGCTT CTGGGCTTCC TCAGAGAACA GGTCCCAGGA GACCTGATTT CTCCAAGCTA 
CCCAGGATAC CAAAGATCCA TCGGGATGGC AATAAGAGCA CGCAGGACCA GGCCCCAGCC 
AGCGGCCACA TTGTCGAGCT CCCTAGCACT TGCATCAGCC GCCTGACTGG CAGGGAAGGC 
CCTGGGCAGC CAGGACGAGG CCGAGTCGAC AGTGAGCCAA GCAGCAGGGG TCCCCAGGAG 
ACTGGCTCAC ACACAAGTGG TTCTCGACCT CCTGCGCCCA GCTCCCATGG CAGCTTAGCC 
CATCTAGGGC CCTCAAGAGG AAAGGGCATT GGGTCTAGCT TTGAAAGTTT CCGGATCAAC 
ATCCCTGGGA ACACTGCACA TTGCAGCCAG CTGTCCAGCC CTGGATTCTG TAACACGTTC 
CGGCCGGTAG ACAGCAAGGT GCAGAGGAAG GAGACCCCGT TCCCCCTCTT CTCCATCAAG 
AAGCCGAAAC AACTCAAAAG CGAAATCTAT GACCCCTTTG ACCCCACTGG CTCGGACTCT 
AGCCCTCCTA GCAGCAGCCC AGAGAGTCTT GGTCCAGGCC TCCTACCCTC TGAGATCACA 
AGAACTATCT CCATCAACAG CCCAAAGGCC CCAGCCTTCC AGACTGTGCG CTGTGTTACC 
TCCTACAGGG TAGAAAGCAT CTTTGGGACT GAGATGGAAC CTGAGCCCCA GCCCCCTAGT 
GAGCCTGTAT CTGGCATGCT GGAGTTACTC AGCAAGGGAT CTGCTGAGGG AACCTCTGAT 
TTGGAGCAAG AGGGACTAGG GGAAATAGAG CCTACAGAGA TTCGGGGCTC CACAGCCCGG 
ACACAGAGGC CACCCCCACC AGACCCTTGG GATGATGAGG ACGAAGTGTC CTGTACACCC 
TTCTTTGGCT CTGAGGAACG GACAGTGACG TGTGTGACTG TTGAGGAGCC AGGTGTTCTA 
CCAAGCCCAG ATGCTCCACA GATCACCACC CACAGAATTG TGGAGTTCAG GGCCTCATCC 
CGTTCCCGCT CCACCTCCAG CTCCCGAAGC AGGAAGAAAA CGAAGAAGAA GAAGAAAAAG 
GTTGCCAGAG AGCACCAGAG GACACGCTCC AGCACTCGCT CTGGCTCCAG GGACAGGACT 
TCACGCTCAG TATCTCCAGT TGCTGAGGAA CACACCAGGA GGCACAGAGC CAAGACTAAG 
AGCCGGAGGT CTTCCAGTGA CCGTGCCAGC AGCCAGGACA GAGCAAAGAG GAGGAAGGAC 
AGAGACGACA GAGACAGGGA GCATAGGCGG GGTTCCTGGG GTCATGGCAG GTGCCGGCGG 
AAGTCCAGGT CTCGCTCAGG GAGCCCTGGC AGTTCTTCCT GTGAGCGCCA TGAGAGTAAG 
AGACGGAAGA GGCGACATTC GGGGTCCAGG TCTCGGGGCA GCAGTCTGGA GAGGGACCGG 
AGACACAAAC ATCGGGAGAG GAGCCGAGAG CGAATGGACA AGCAGGAGAG TGTGACCCGG 
TCCCGGGAGA GGAGGAGGTG GCGATCTCGG TCGCCTAGCT TGGAGCATAG GCCACGAAGG 
CCACCTTCCC GTGAGAAGCG TGCCCATTCC CCAGAGAAAA AGGGGCCTGT GAGGGAGGTT 
TCCCCAGCCC CTGCTACACA GGGGGAGTCG AGGCAGGATG GAGACCACTC TGCTGAGCCT 
CCGGTTTCAG AAGTAAGTGT CTTGCCTGAG GTAGTAAGTG TCCTGCCAGA GGTGGTTGTG 
GCTGACCTGA ACCCTCCAGA AGTCCCTCCT GTCCTGGCAG AGCCAGTTGC CCATGTGCCT 
GAGGACCTGG ACTATGGTGA GTCTGTGGAG GCAGGCCACG TCTTTGAGGA TTTTTCAAAT 
GAAGCCATCT TCATCCAGCT CGATGACATG AGCTCGCCTC CTTCTCCTGA GAGTACAGAC 
TCCTCCCCTG AGAGAGACTT CCCACCAAAT CCCATACTGC CCCCAGCCAG CCTTCCACAA 
GACAGTACTT TACCTACCAT CCAAAGGGAG GTGTTACCCA TTCACAGTGA AGATATCTCA 
AAGCCTGTGC CCCAGGCCTT GGCCCCATCA GACCAGAGTC TGCTCAAGCA AGACACTGTA 
GAGATCACCA CTACAACTCC CAGCACCCCA GCTGTGGTGC CCATGACAAA GGACAGTCCG 
GTGCTGAGTG CAAGAGGATG GGAAGCAGTC AGGCCCAGGG ATGCTGTGGC CCAGGCCCCA 
CTGCTACGAT CCCGAACCCT GGTGAAAAGA GTCACCTGGA ACCTGCAGGA AGCAGAACAC 
AGCACGCCAG CAGCCCTGGA CAGAGATCCA AGGTTGACCA CAAGGCATCC ACACTCCCTG 
TCTGTTGGGT CCTCAGGGAC ACCACTTCAG AGGCCCCAGA GGCCCCAGGA AGGAGACTGG 
GATGCAGAGG ACAGGGCCCT CATAGGATTT CAGCAGGCAC CATTCTCCGA GCTGCCCCCT 
CCCATCCACG TGCTCCAGGA GTCTGGGTTA CCTGATGCAG ATCCCTCTCA GCCCCCTGGT 
GCTCCCAGGG CAGAAGGGCT TCCAGCTGCT GGGACTCTGC ACTCAGCAGG GGGTATTCTT 
GCCCAGGTTT ACAGCCCCAA CATGCCACCA CCCTTGGCTC AGCCCTCAAG CATCCTGCCC 
TATGCACTGG TCAGCCAGCC CTCAGTCCAG TTGATCCTGC AGGGGACCCT TCCCCTAGCA 
GGCTGCGGTA CAGCACAGAG CCTGGCACCA GTGCCCACAA TGCCAGCTAC AGTCTCAGAG 
CTAGCTGTTC CAACCACCAA CAACTCAGAA GAAAGGACTG CTACTCCCAA AACAGCTGCT 
GAGAAGACCA AAAAGGAGGA GTACATGAAG AAGCTGCACA TGCAAGAACG GGCTGTGGAG 
GAGGTGAAGC TGGCCATTAA ACCCTTCTAC CAGAAGAGAG AAGTGACCAA GGAGGAGTAC 
AAGGACATAC TGCGCAAAGC CGTGCAGAAG ATCTGCCACA GCAAGAGTGG TGAAATCAAC 
CCTGTAAAGG TGGCCAACCT GGTGAAGGCC TATGTGGACA AATACAGGCA TATGCGCAGA 
CACAAGAAGA CTGAAGGTGG AGAGGAGCCA CCCACTCAGG GTGCTGAGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536142.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536205.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.

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CloneID	OMu40029
Clone ID Related Accession (Same CDS sequence)	XM_006536142.1 , XM_006536142.2 , XM_030241933.1
Accession Version	XM_006536142.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536205.1
CDS	348..5438
Translation	MDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQPPGAPRAEGLPAAGTLHSAGGILAQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536142.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536142.1

ORF Insert Sequence:

ATGGATGACG ACAACTTGGA TGAGCTTGTG GCGCACAGCC CTGGGCCAGA TGGACCCCCA 
CGGGTTGGCT CCTCAGAACT GGCCAGTGAT GCTGAAAGCA GCAATGGCCA AAGTGGAGAC 
TCTGAAGATG ATACAGGCAG TGAGCAGGAT GATGACACAG ATGGGGAGGA AACAGAGGGG 
CTGTCTGAAG AGGAAGACCC AGAGGATAGA TCTGGTTCTG AAGACTCAGA AGATGGAGTA 
GAAATGGCAA CGGCAGCAAT AGAGACTCAA GGGAAGCTTG AAGCTAGCAG TGTACCCAAT 
TCTGATGATG ATGCAGAGAG CTGCCCCATT TGCCTCAATG CATTTAGAGA CCAGGCTGTG 
GGCACCCCAG AGACCTGTGC CCATTATTTC TGCCTGGATT GCATCATCGA ATGGTCCAGG 
AATGCCAACT CCTGTCCTGT TGATCGAACA GTATTTAAAT GTATTTGTAT TCGTGCCCAG 
TTTAATGGTA AAATCTTAAA GAAGATTCCA GTAGAAAACA CTAAAGCTTG TGAGGCTGAG 
GAGGAAGACC CGACTTTTTG TGAGGTGTGT GGCAGGAGTG ATCGTGAGGA CCGGCTCCTA 
CTGTGTGACG GCTGCGATGC TGGGTACCAC ATGGAATGTT TGGACCCGCC TCTCCAGGAG 
GTGCCTGTGG ATGAATGGTT CTGCCCAGAG TGTACGGTCC CTGGTGTGGA TCCCACTCAT 
GATGCAGCTC CTGTGAGTGA TGAGGAGGTC TCCTTGCTAC TGGCTGATGT AGTACCTACT 
ACAAGCAGGC TTCGGCCTCG AGTAGGCAGG ACCCGGGCCA TCGCTAGGAC ACGGCAGAGT 
GAGAGAGTGA GGGCAACTGT GAACCGGAAC CGGATCTCCT CTGCCAGAAG GGTCCAGCAT 
GTGCCAAGGT ACCTCATGTC TTCTCTGCTG GATGAAACTA TTGAGGCTGT CGCCACTGGG 
CTGAGCACTG CTGTATACCA GCGTCCCTTG ACACCTCGTG TCCCTGCAAA ACGGAAAAGG 
AAGGCAGGGA GACGAAAGAA AGTGCTGGGA AGAAAGAAAA CTCGGTCCAG GTCATCTGTG 
AAAAGTAAGA GTGGGAGTAC GAGAGCCAAG AAACGTCAGC ACCGTGTAAG GAAGACAAAA 
GGGAGGAAGC TGAAGAATGA AGTCACAGCT CGTTCTCGAA TTGCACGAAC ACTAGGCCTC 
CGCAGGCCTG TCCGTGGCAC CAGCATGCCA TCAGTGTACA AGCCAGTGGA CCCCTCCCTC 
GGGCTGATGA GGGCAGACAT TGGAGCAGCT TCCCTGTCAC TGTTTGGAGA TCCCTATGAG 
CTGGATCCTT TTGACAGCAA TGAAGAACAG TCTGCAGATC CACCTTCCCC TCTGAGTGCC 
AAGAGGAGAG TGCTGTCCCG CTCAGCTCTG CAGTCTCACC AGCCTGTGGC CAGACCTGTC 
GCCATGGGGC TCTCTAGGAG GCAGCTCCCT GCTGTGGCCC CAGAGCCCAG CGTGGAGGAG 
GCCCCCGTTC CTGACCTGTT GGGAAGCATC CTGTCTGGCC AGAGCCTCCT GATGATGAGC 
AGTGCTGATG TTGTCATACA CCGGGATGGC TCTCTCAGCG CCAAGAGGGC AGCTCCTGTT 
TCTCTTCAGC GAAATTCTGT GACTCAATCC AGAGAAGAGT CCAGGCTCAG AGACAACCCG 
CAGCCTGGGG CACTACCCTC AGAGAGTGCA TCTGGTGGAT TCGTGGGAGA CAGACAACCA 
AACTCAGGGC TGAGCTGTGG GAATAGAACA GCTCTGTGCT GTCTCCCTGC CCGCATTGCC 
CAGACACCTG TGAGGTCAGA TCCCTCACTG ACTCCTCGCT CAGGTCTGTC TAGGACCCTT 
TCAGATGAGA ACAGGCCCAG CCGGACACAC AGCAGCAGCC CCCAACTCAA TGGCTCCAAT 
GTACGGGTTA GTTCTGCCTC GACTAAGATC GTGACTCATT CTAGCTTTCC ATCGAAAAAT 
ACAGCTTCTG GGCTTCCTCA GAGAACAGGT CCCAGGAGAC CTGATTTCTC CAAGCTACCC 
AGGATACCAA AGATCCATCG GGATGGCAAT AAGAGCACGC AGGACCAGGC CCCAGCCAGC 
GGCCACATTG TCGAGCTCCC TAGCACTTGC ATCAGCCGCC TGACTGGCAG GGAAGGCCCT 
GGGCAGCCAG GACGAGGCCG AGTCGACAGT GAGCCAAGCA GCAGGGGTCC CCAGGAGACT 
GGCTCACACA CAAGTGGTTC TCGACCTCCT GCGCCCAGCT CCCATGGCAG CTTAGCCCAT 
CTAGGGCCCT CAAGAGGAAA GGGCATTGGG TCTAGCTTTG AAAGTTTCCG GATCAACATC 
CCTGGGAACA CTGCACATTG CAGCCAGCTG TCCAGCCCTG GATTCTGTAA CACGTTCCGG 
CCGGTAGACA GCAAGGTGCA GAGGAAGGAG ACCCCGTTCC CCCTCTTCTC CATCAAGAAG 
CCGAAACAAC TCAAAAGCGA AATCTATGAC CCCTTTGACC CCACTGGCTC GGACTCTAGC 
CCTCCTAGCA GCAGCCCAGA GAGTCTTGGT CCAGGCCTCC TACCCTCTGA GATCACAAGA 
ACTATCTCCA TCAACAGCCC AAAGGCCCCA GCCTTCCAGA CTGTGCGCTG TGTTACCTCC 
TACAGGGTAG AAAGCATCTT TGGGACTGAG ATGGAACCTG AGCCCCAGCC CCCTAGTGAG 
CCTGTATCTG GCATGCTGGA GTTACTCAGC AAGGGATCTG CTGAGGGAAC CTCTGATTTG 
GAGCAAGAGG GACTAGGGGA AATAGAGCCT ACAGAGATTC GGGGCTCCAC AGCCCGGACA 
CAGAGGCCAC CCCCACCAGA CCCTTGGGAT GATGAGGACG AAGTGTCCTG TACACCCTTC 
TTTGGCTCTG AGGAACGGAC AGTGACGTGT GTGACTGTTG AGGAGCCAGG TGTTCTACCA 
AGCCCAGATG CTCCACAGAT CACCACCCAC AGAATTGTGG AGTTCAGGGC CTCATCCCGT 
TCCCGCTCCA CCTCCAGCTC CCGAAGCAGG AAGAAAACGA AGAAGAAGAA GAAAAAGGTT 
GCCAGAGAGC ACCAGAGGAC ACGCTCCAGC ACTCGCTCTG GCTCCAGGGA CAGGACTTCA 
CGCTCAGTAT CTCCAGTTGC TGAGGAACAC ACCAGGAGGC ACAGAGCCAA GACTAAGAGC 
CGGAGGTCTT CCAGTGACCG TGCCAGCAGC CAGGACAGAG CAAAGAGGAG GAAGGACAGA 
GACGACAGAG ACAGGGAGCA TAGGCGGGGT TCCTGGGGTC ATGGCAGGTG CCGGCGGAAG 
TCCAGGTCTC GCTCAGGGAG CCCTGGCAGT TCTTCCTGTG AGCGCCATGA GAGTAAGAGA 
CGGAAGAGGC GACATTCGGG GTCCAGGTCT CGGGGCAGCA GTCTGGAGAG GGACCGGAGA 
CACAAACATC GGGAGAGGAG CCGAGAGCGA ATGGACAAGC AGGAGAGTGT GACCCGGTCC 
CGGGAGAGGA GGAGGTGGCG ATCTCGGTCG CCTAGCTTGG AGCATAGGCC ACGAAGGCCA 
CCTTCCCGTG AGAAGCGTGC CCATTCCCCA GAGAAAAAGG GGCCTGTGAG GGAGGTTTCC 
CCAGCCCCTG CTACACAGGG GGAGTCGAGG CAGGATGGAG ACCACTCTGC TGAGCCTCCG 
GTTTCAGAAG TAAGTGTCTT GCCTGAGGTA GTAAGTGTCC TGCCAGAGGT GGTTGTGGCT 
GACCTGAACC CTCCAGAAGT CCCTCCTGTC CTGGCAGAGC CAGTTGCCCA TGTGCCTGAG 
GACCTGGACT ATGGTGAGTC TGTGGAGGCA GGCCACGTCT TTGAGGATTT TTCAAATGAA 
GCCATCTTCA TCCAGCTCGA TGACATGAGC TCGCCTCCTT CTCCTGAGAG TACAGACTCC 
TCCCCTGAGA GAGACTTCCC ACCAAATCCC ATACTGCCCC CAGCCAGCCT TCCACAAGAC 
AGTACTTTAC CTACCATCCA AAGGGAGGTG TTACCCATTC ACAGTGAAGA TATCTCAAAG 
CCTGTGCCCC AGGCCTTGGC CCCATCAGAC CAGAGTCTGC TCAAGCAAGA CACTGTAGAG 
ATCACCACTA CAACTCCCAG CACCCCAGCT GTGGTGCCCA TGACAAAGGA CAGTCCGGTG 
CTGAGTGCAA GAGGATGGGA AGCAGTCAGG CCCAGGGATG CTGTGGCCCA GGCCCCACTG 
CTACGATCCC GAACCCTGGT GAAAAGAGTC ACCTGGAACC TGCAGGAAGC AGAACACAGC 
ACGCCAGCAG CCCTGGACAG AGATCCAAGG TTGACCACAA GGCATCCACA CTCCCTGTCT 
GTTGGGTCCT CAGGGACACC ACTTCAGAGG CCCCAGAGGC CCCAGGAAGG AGACTGGGAT 
GCAGAGGACA GGGCCCTCAT AGGATTTCAG CAGGCACCAT TCTCCGAGCT GCCCCCTCCC 
ATCCACGTGC TCCAGGAGTC TGGGTTACCT GATGCAGATC CCTCTCAGCC CCCTGGTGCT 
CCCAGGGCAG AAGGGCTTCC AGCTGCTGGG ACTCTGCACT CAGCAGGGGG TATTCTTGCC 
CAGGTTTACA GCCCCAACAT GCCACCACCC TTGGCTCAGC CCTCAAGCAT CCTGCCCTAT 
GCACTGGTCA GCCAGCCCTC AGTCCAGTTG ATCCTGCAGG GGACCCTTCC CCTAGCAGGC 
TGCGGTACAG CACAGAGCCT GGCACCAGTG CCCACAATGC CAGCTACAGT CTCAGAGCTA 
GCTGTTCCAA CCACCAACAA CTCAGAAGAA AGGACTGCTA CTCCCAAAAC AGCTGCTGAG 
AAGACCAAAA AGGAGGAGTA CATGAAGAAG CTGCACATGC AAGAACGGGC TGTGGAGGAG 
GTGAAGCTGG CCATTAAACC CTTCTACCAG AAGAGAGAAG TGACCAAGGA GGAGTACAAG 
GACATACTGC GCAAAGCCGT GCAGAAGATC TGCCACAGCA AGAGTGGTGA AATCAACCCT 
GTAAAGGTGG CCAACCTGGT GAAGGCCTAT GTGGACAAAT ACAGGCATAT GCGCAGACAC 
AAGAAGACTG AAGGTGGAGA GGAGCCACCC ACTCAGGGTG CTGAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536143.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536206.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.

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CloneID	OMu40030
Clone ID Related Accession (Same CDS sequence)	XM_006536143.3 , XM_006536143.4
Accession Version	XM_006536143.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGGTTTA CAGCCCCAAC 
ATGCCACCAC CCTTGGCTCA GCCCTCAAGC ATCCTGCCCT ATGCACTGGT CAGCCAGCCC 
TCAGTCCAGT TGATCCTGCA GGGGACCCTT CCCCTAGCAG GCTGCGGTAC AGCACAGAGC 
CTGGCACCAG TGCCCACAAT GCCAGCTACA GTCTCAGAGC TAGCTGTTCC AACCACCAAC 
AACTCAGAAG AAAGGACTGC TACTCCCAAA ACAGCTGCTG AGAAGACCAA AAAGGAGGAG 
TACATGAAGA AGCTGCACAT GCAAGAACGG GCTGTGGAGG AGGTGAAGCT GGCCATTAAA 
CCCTTCTACC AGAAGAGAGA AGTGACCAAG GAGGAGTACA AGGACATACT GCGCAAAGCC 
GTGCAGAAGA TCTGCCACAG CAAGAGTGGT GAAATCAACC CTGTAAAGGT GGCCAACCTG 
GTGAAGGCCT ATGTGGACAA ATACAGGCAT ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA 
GAGGAGCCAC CCACTCAGGG TGCTGAGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536206.1
CDS	255..5327
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRLTTRHPHSLSVGSSGTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536143.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536143.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGTTG ACCACAAGGC ATCCACACTC CCTGTCTGTT 
GGGTCCTCAG GGACACCACT TCAGAGGCCC CAGAGGCCCC AGGAAGGAGA CTGGGATGCA 
GAGGACAGGG CCCTCATAGG ATTTCAGCAG GCACCATTCT CCGAGCTGCC CCCTCCCATC 
CACGTGCTCC AGGAGTCTGG GTTACCTGAT GCAGATCCCT CTCAGGTTTA CAGCCCCAAC 
ATGCCACCAC CCTTGGCTCA GCCCTCAAGC ATCCTGCCCT ATGCACTGGT CAGCCAGCCC 
TCAGTCCAGT TGATCCTGCA GGGGACCCTT CCCCTAGCAG GCTGCGGTAC AGCACAGAGC 
CTGGCACCAG TGCCCACAAT GCCAGCTACA GTCTCAGAGC TAGCTGTTCC AACCACCAAC 
AACTCAGAAG AAAGGACTGC TACTCCCAAA ACAGCTGCTG AGAAGACCAA AAAGGAGGAG 
TACATGAAGA AGCTGCACAT GCAAGAACGG GCTGTGGAGG AGGTGAAGCT GGCCATTAAA 
CCCTTCTACC AGAAGAGAGA AGTGACCAAG GAGGAGTACA AGGACATACT GCGCAAAGCC 
GTGCAGAAGA TCTGCCACAG CAAGAGTGGT GAAATCAACC CTGTAAAGGT GGCCAACCTG 
GTGAAGGCCT ATGTGGACAA ATACAGGCAT ATGCGCAGAC ACAAGAAGAC TGAAGGTGGA 
GAGGAGCCAC CCACTCAGGG TGCTGAGACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536144.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536207.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.

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CloneID	OMu40031
Clone ID Related Accession (Same CDS sequence)	XM_006536144.3 , XM_006536144.4
Accession Version	XM_006536144.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536207.1
CDS	255..5282
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAEESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536144.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536144.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA GAAAGCAGCA ATGGCCAAAG TGGAGACTCT 
GAAGATGATA CAGGCAGTGA GCAGGATGAT GACACAGATG GGGAGGAAAC AGAGGGGCTG 
TCTGAAGAGG AAGACCCAGA GGATAGATCT GGTTCTGAAG ACTCAGAAGA TGGAGTAGAA 
ATGGCAACGG CAGCAATAGA GACTCAAGGG AAGCTTGAAG CTAGCAGTGT ACCCAATTCT 
GATGATGATG CAGAGAGCTG CCCCATTTGC CTCAATGCAT TTAGAGACCA GGCTGTGGGC 
ACCCCAGAGA CCTGTGCCCA TTATTTCTGC CTGGATTGCA TCATCGAATG GTCCAGGAAT 
GCCAACTCCT GTCCTGTTGA TCGAACAGTA TTTAAATGTA TTTGTATTCG TGCCCAGTTT 
AATGGTAAAA TCTTAAAGAA GATTCCAGTA GAAAACACTA AAGCTTGTGA GGCTGAGGAG 
GAAGACCCGA CTTTTTGTGA GGTGTGTGGC AGGAGTGATC GTGAGGACCG GCTCCTACTG 
TGTGACGGCT GCGATGCTGG GTACCACATG GAATGTTTGG ACCCGCCTCT CCAGGAGGTG 
CCTGTGGATG AATGGTTCTG CCCAGAGTGT ACGGTCCCTG GTGTGGATCC CACTCATGAT 
GCAGCTCCTG TGAGTGATGA GGAGGTCTCC TTGCTACTGG CTGATGTAGT ACCTACTACA 
AGCAGGCTTC GGCCTCGAGT AGGCAGGACC CGGGCCATCG CTAGGACACG GCAGAGTGAG 
AGAGTGAGGG CAACTGTGAA CCGGAACCGG ATCTCCTCTG CCAGAAGGGT CCAGCATGTG 
CCAAGGTACC TCATGTCTTC TCTGCTGGAT GAAACTATTG AGGCTGTCGC CACTGGGCTG 
AGCACTGCTG TATACCAGCG TCCCTTGACA CCTCGTGTCC CTGCAAAACG GAAAAGGAAG 
GCAGGGAGAC GAAAGAAAGT GCTGGGAAGA AAGAAAACTC GGTCCAGGTC ATCTGTGAAA 
AGTAAGAGTG GGAGTACGAG AGCCAAGAAA CGTCAGCACC GTGTAAGGAA GACAAAAGGG 
AGGAAGCTGA AGAATGAAGT CACAGCTCGT TCTCGAATTG CACGAACACT AGGCCTCCGC 
AGGCCTGTCC GTGGCACCAG CATGCCATCA GTGTACAAGC CAGTGGACCC CTCCCTCGGG 
CTGATGAGGG CAGACATTGG AGCAGCTTCC CTGTCACTGT TTGGAGATCC CTATGAGCTG 
GATCCTTTTG ACAGCAATGA AGAACAGTCT GCAGATCCAC CTTCCCCTCT GAGTGCCAAG 
AGGAGAGTGC TGTCCCGCTC AGCTCTGCAG TCTCACCAGC CTGTGGCCAG ACCTGTCGCC 
ATGGGGCTCT CTAGGAGGCA GCTCCCTGCT GTGGCCCCAG AGCCCAGCGT GGAGGAGGCC 
CCCGTTCCTG ACCTGTTGGG AAGCATCCTG TCTGGCCAGA GCCTCCTGAT GATGAGCAGT 
GCTGATGTTG TCATACACCG GGATGGCTCT CTCAGCGCCA AGAGGGCAGC TCCTGTTTCT 
CTTCAGCGAA ATTCTGTGAC TCAATCCAGA GAAGAGTCCA GGCTCAGAGA CAACCCGCAG 
CCTGGGGCAC TACCCTCAGA GAGTGCATCT GGTGGATTCG TGGGAGACAG ACAACCAAAC 
TCAGGGCTGA GCTGTGGGAA TAGAACAGCT CTGTGCTGTC TCCCTGCCCG CATTGCCCAG 
ACACCTGTGA GGTCAGATCC CTCACTGACT CCTCGCTCAG GTCTGTCTAG GACCCTTTCA 
GATGAGAACA GGCCCAGCCG GACACACAGC AGCAGCCCCC AACTCAATGG CTCCAATGTA 
CGGGTTAGTT CTGCCTCGAC TAAGATCGTG ACTCATTCTA GCTTTCCATC GAAAAATACA 
GCTTCTGGGC TTCCTCAGAG AACAGGTCCC AGGAGACCTG ATTTCTCCAA GCTACCCAGG 
ATACCAAAGA TCCATCGGGA TGGCAATAAG AGCACGCAGG ACCAGGCCCC AGCCAGCGGC 
CACATTGTCG AGCTCCCTAG CACTTGCATC AGCCGCCTGA CTGGCAGGGA AGGCCCTGGG 
CAGCCAGGAC GAGGCCGAGT CGACAGTGAG CCAAGCAGCA GGGGTCCCCA GGAGACTGGC 
TCACACACAA GTGGTTCTCG ACCTCCTGCG CCCAGCTCCC ATGGCAGCTT AGCCCATCTA 
GGGCCCTCAA GAGGAAAGGG CATTGGGTCT AGCTTTGAAA GTTTCCGGAT CAACATCCCT 
GGGAACACTG CACATTGCAG CCAGCTGTCC AGCCCTGGAT TCTGTAACAC GTTCCGGCCG 
GTAGACAGCA AGGTGCAGAG GAAGGAGACC CCGTTCCCCC TCTTCTCCAT CAAGAAGCCG 
AAACAACTCA AAAGCGAAAT CTATGACCCC TTTGACCCCA CTGGCTCGGA CTCTAGCCCT 
CCTAGCAGCA GCCCAGAGAG TCTTGGTCCA GGCCTCCTAC CCTCTGAGAT CACAAGAACT 
ATCTCCATCA ACAGCCCAAA GGCCCCAGCC TTCCAGACTG TGCGCTGTGT TACCTCCTAC 
AGGGTAGAAA GCATCTTTGG GACTGAGATG GAACCTGAGC CCCAGCCCCC TAGTGAGCCT 
GTATCTGGCA TGCTGGAGTT ACTCAGCAAG GGATCTGCTG AGGGAACCTC TGATTTGGAG 
CAAGAGGGAC TAGGGGAAAT AGAGCCTACA GAGATTCGGG GCTCCACAGC CCGGACACAG 
AGGCCACCCC CACCAGACCC TTGGGATGAT GAGGACGAAG TGTCCTGTAC ACCCTTCTTT 
GGCTCTGAGG AACGGACAGT GACGTGTGTG ACTGTTGAGG AGCCAGGTGT TCTACCAAGC 
CCAGATGCTC CACAGATCAC CACCCACAGA ATTGTGGAGT TCAGGGCCTC ATCCCGTTCC 
CGCTCCACCT CCAGCTCCCG AAGCAGGAAG AAAACGAAGA AGAAGAAGAA AAAGGTTGCC 
AGAGAGCACC AGAGGACACG CTCCAGCACT CGCTCTGGCT CCAGGGACAG GACTTCACGC 
TCAGTATCTC CAGTTGCTGA GGAACACACC AGGAGGCACA GAGCCAAGAC TAAGAGCCGG 
AGGTCTTCCA GTGACCGTGC CAGCAGCCAG GACAGAGCAA AGAGGAGGAA GGACAGAGAC 
GACAGAGACA GGGAGCATAG GCGGGGTTCC TGGGGTCATG GCAGGTGCCG GCGGAAGTCC 
AGGTCTCGCT CAGGGAGCCC TGGCAGTTCT TCCTGTGAGC GCCATGAGAG TAAGAGACGG 
AAGAGGCGAC ATTCGGGGTC CAGGTCTCGG GGCAGCAGTC TGGAGAGGGA CCGGAGACAC 
AAACATCGGG AGAGGAGCCG AGAGCGAATG GACAAGCAGG AGAGTGTGAC CCGGTCCCGG 
GAGAGGAGGA GGTGGCGATC TCGGTCGCCT AGCTTGGAGC ATAGGCCACG AAGGCCACCT 
TCCCGTGAGA AGCGTGCCCA TTCCCCAGAG AAAAAGGGGC CTGTGAGGGA GGTTTCCCCA 
GCCCCTGCTA CACAGGGGGA GTCGAGGCAG GATGGAGACC ACTCTGCTGA GCCTCCGGTT 
TCAGAAGTAA GTGTCTTGCC TGAGGTAGTA AGTGTCCTGC CAGAGGTGGT TGTGGCTGAC 
CTGAACCCTC CAGAAGTCCC TCCTGTCCTG GCAGAGCCAG TTGCCCATGT GCCTGAGGAC 
CTGGACTATG GTGAGTCTGT GGAGGCAGGC CACGTCTTTG AGGATTTTTC AAATGAAGCC 
ATCTTCATCC AGCTCGATGA CATGAGCTCG CCTCCTTCTC CTGAGAGTAC AGACTCCTCC 
CCTGAGAGAG ACTTCCCACC AAATCCCATA CTGCCCCCAG CCAGCCTTCC ACAAGACAGT 
ACTTTACCTA CCATCCAAAG GGAGGTGTTA CCCATTCACA GTGAAGATAT CTCAAAGCCT 
GTGCCCCAGG CCTTGGCCCC ATCAGACCAG AGTCTGCTCA AGCAAGACAC TGTAGAGATC 
ACCACTACAA CTCCCAGCAC CCCAGCTGTG GTGCCCATGA CAAAGGACAG TCCGGTGCTG 
AGTGCAAGAG GATGGGAAGC AGTCAGGCCC AGGGATGCTG TGGCCCAGGC CCCACTGCTA 
CGATCCCGAA CCCTGGTGAA AAGAGTCACC TGGAACCTGC AGGAAGCAGA ACACAGCACG 
CCAGCAGCCC TGGACAGAGA TCCAAGGACA CCACTTCAGA GGCCCCAGAG GCCCCAGGAA 
GGAGACTGGG ATGCAGAGGA CAGGGCCCTC ATAGGATTTC AGCAGGCACC ATTCTCCGAG 
CTGCCCCCTC CCATCCACGT GCTCCAGGAG TCTGGGTTAC CTGATGCAGA TCCCTCTCAG 
GTTTACAGCC CCAACATGCC ACCACCCTTG GCTCAGCCCT CAAGCATCCT GCCCTATGCA 
CTGGTCAGCC AGCCCTCAGT CCAGTTGATC CTGCAGGGGA CCCTTCCCCT AGCAGGCTGC 
GGTACAGCAC AGAGCCTGGC ACCAGTGCCC ACAATGCCAG CTACAGTCTC AGAGCTAGCT 
GTTCCAACCA CCAACAACTC AGAAGAAAGG ACTGCTACTC CCAAAACAGC TGCTGAGAAG 
ACCAAAAAGG AGGAGTACAT GAAGAAGCTG CACATGCAAG AACGGGCTGT GGAGGAGGTG 
AAGCTGGCCA TTAAACCCTT CTACCAGAAG AGAGAAGTGA CCAAGGAGGA GTACAAGGAC 
ATACTGCGCA AAGCCGTGCA GAAGATCTGC CACAGCAAGA GTGGTGAAAT CAACCCTGTA 
AAGGTGGCCA ACCTGGTGAA GGCCTATGTG GACAAATACA GGCATATGCG CAGACACAAG 
AAGACTGAAG GTGGAGAGGA GCCACCCACT CAGGGTGCTG AGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phrf1 ( XM_006536145.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006536208.1 Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.

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CloneID	OMu40032
Clone ID Related Accession (Same CDS sequence)	XM_006536145.4 , XM_006536145.3
Accession Version	XM_006536145.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PHD and RING finger domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006536145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGACACCA CTTCAGAGGC CCCAGAGGCC CCAGGAAGGA 
GACTGGGATG CAGAGGACAG GGCCCTCATA GGATTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGATCC CTCTCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006536208.1
CDS	255..5279
Translation	MGSRTVPTAWRGELQCAAMDDDNLDELVAHSPGPDGPPRVGSSELASDAESSNGQSGDSEDDTGSEQDDDTDGEETEGLSEEEDPEDRSGSEDSEDGVEMATAAIETQGKLEASSVPNSDDDAESCPICLNAFRDQAVGTPETCAHYFCLDCIIEWSRNANSCPVDRTVFKCICIRAQFNGKILKKIPVENTKACEAEEEDPTFCEVCGRSDREDRLLLCDGCDAGYHMECLDPPLQEVPVDEWFCPECTVPGVDPTHDAAPVSDEEVSLLLADVVPTTSRLRPRVGRTRAIARTRQSERVRATVNRNRISSARRVQHVPRYLMSSLLDETIEAVATGLSTAVYQRPLTPRVPAKRKRKAGRRKKVLGRKKTRSRSSVKSKSGSTRAKKRQHRVRKTKGRKLKNEVTARSRIARTLGLRRPVRGTSMPSVYKPVDPSLGLMRADIGAASLSLFGDPYELDPFDSNEEQSADPPSPLSAKRRVLSRSALQSHQPVARPVAMGLSRRQLPAVAPEPSVEEAPVPDLLGSILSGQSLLMMSSADVVIHRDGSLSAKRAAPVSLQRNSVTQSREESRLRDNPQPGALPSESASGGFVGDRQPNSGLSCGNRTALCCLPARIAQTPVRSDPSLTPRSGLSRTLSDENRPSRTHSSSPQLNGSNVRVSSASTKIVTHSSFPSKNTASGLPQRTGPRRPDFSKLPRIPKIHRDGNKSTQDQAPASGHIVELPSTCISRLTGREGPGQPGRGRVDSEPSSRGPQETGSHTSGSRPPAPSSHGSLAHLGPSRGKGIGSSFESFRINIPGNTAHCSQLSSPGFCNTFRPVDSKVQRKETPFPLFSIKKPKQLKSEIYDPFDPTGSDSSPPSSSPESLGPGLLPSEITRTISINSPKAPAFQTVRCVTSYRVESIFGTEMEPEPQPPSEPVSGMLELLSKGSAEGTSDLEQEGLGEIEPTEIRGSTARTQRPPPPDPWDDEDEVSCTPFFGSEERTVTCVTVEEPGVLPSPDAPQITTHRIVEFRASSRSRSTSSSRSRKKTKKKKKKVAREHQRTRSSTRSGSRDRTSRSVSPVAEEHTRRHRAKTKSRRSSSDRASSQDRAKRRKDRDDRDREHRRGSWGHGRCRRKSRSRSGSPGSSSCERHESKRRKRRHSGSRSRGSSLERDRRHKHRERSRERMDKQESVTRSRERRRWRSRSPSLEHRPRRPPSREKRAHSPEKKGPVREVSPAPATQGESRQDGDHSAEPPVSEVSVLPEVVSVLPEVVVADLNPPEVPPVLAEPVAHVPEDLDYGESVEAGHVFEDFSNEAIFIQLDDMSSPPSPESTDSSPERDFPPNPILPPASLPQDSTLPTIQREVLPIHSEDISKPVPQALAPSDQSLLKQDTVEITTTTPSTPAVVPMTKDSPVLSARGWEAVRPRDAVAQAPLLRSRTLVKRVTWNLQEAEHSTPAALDRDPRTPLQRPQRPQEGDWDAEDRALIGFQQAPFSELPPPIHVLQESGLPDADPSQVYSPNMPPPLAQPSSILPYALVSQPSVQLILQGTLPLAGCGTAQSLAPVPTMPATVSELAVPTTNNSEERTATPKTAAEKTKKEEYMKKLHMQERAVEEVKLAIKPFYQKREVTKEEYKDILRKAVQKICHSKSGEINPVKVANLVKAYVDKYRHMRRHKKTEGGEEPPTQGAET

Target ORF information:

RefSeq Version	XM_006536145.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PHD and ring finger domains 1 (Phrf1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006536145.3

ORF Insert Sequence:

ATGGGGAGTC GGACTGTACC AACTGCATGG AGAGGTGAAC TCCAATGTGC AGCCATGGAT 
GACGACAACT TGGATGAGCT TGTGGCGCAC AGCCCTGGGC CAGATGGACC CCCACGGGTT 
GGCTCCTCAG AACTGGCCAG TGATGCTGAA AGCAGCAATG GCCAAAGTGG AGACTCTGAA 
GATGATACAG GCAGTGAGCA GGATGATGAC ACAGATGGGG AGGAAACAGA GGGGCTGTCT 
GAAGAGGAAG ACCCAGAGGA TAGATCTGGT TCTGAAGACT CAGAAGATGG AGTAGAAATG 
GCAACGGCAG CAATAGAGAC TCAAGGGAAG CTTGAAGCTA GCAGTGTACC CAATTCTGAT 
GATGATGCAG AGAGCTGCCC CATTTGCCTC AATGCATTTA GAGACCAGGC TGTGGGCACC 
CCAGAGACCT GTGCCCATTA TTTCTGCCTG GATTGCATCA TCGAATGGTC CAGGAATGCC 
AACTCCTGTC CTGTTGATCG AACAGTATTT AAATGTATTT GTATTCGTGC CCAGTTTAAT 
GGTAAAATCT TAAAGAAGAT TCCAGTAGAA AACACTAAAG CTTGTGAGGC TGAGGAGGAA 
GACCCGACTT TTTGTGAGGT GTGTGGCAGG AGTGATCGTG AGGACCGGCT CCTACTGTGT 
GACGGCTGCG ATGCTGGGTA CCACATGGAA TGTTTGGACC CGCCTCTCCA GGAGGTGCCT 
GTGGATGAAT GGTTCTGCCC AGAGTGTACG GTCCCTGGTG TGGATCCCAC TCATGATGCA 
GCTCCTGTGA GTGATGAGGA GGTCTCCTTG CTACTGGCTG ATGTAGTACC TACTACAAGC 
AGGCTTCGGC CTCGAGTAGG CAGGACCCGG GCCATCGCTA GGACACGGCA GAGTGAGAGA 
GTGAGGGCAA CTGTGAACCG GAACCGGATC TCCTCTGCCA GAAGGGTCCA GCATGTGCCA 
AGGTACCTCA TGTCTTCTCT GCTGGATGAA ACTATTGAGG CTGTCGCCAC TGGGCTGAGC 
ACTGCTGTAT ACCAGCGTCC CTTGACACCT CGTGTCCCTG CAAAACGGAA AAGGAAGGCA 
GGGAGACGAA AGAAAGTGCT GGGAAGAAAG AAAACTCGGT CCAGGTCATC TGTGAAAAGT 
AAGAGTGGGA GTACGAGAGC CAAGAAACGT CAGCACCGTG TAAGGAAGAC AAAAGGGAGG 
AAGCTGAAGA ATGAAGTCAC AGCTCGTTCT CGAATTGCAC GAACACTAGG CCTCCGCAGG 
CCTGTCCGTG GCACCAGCAT GCCATCAGTG TACAAGCCAG TGGACCCCTC CCTCGGGCTG 
ATGAGGGCAG ACATTGGAGC AGCTTCCCTG TCACTGTTTG GAGATCCCTA TGAGCTGGAT 
CCTTTTGACA GCAATGAAGA ACAGTCTGCA GATCCACCTT CCCCTCTGAG TGCCAAGAGG 
AGAGTGCTGT CCCGCTCAGC TCTGCAGTCT CACCAGCCTG TGGCCAGACC TGTCGCCATG 
GGGCTCTCTA GGAGGCAGCT CCCTGCTGTG GCCCCAGAGC CCAGCGTGGA GGAGGCCCCC 
GTTCCTGACC TGTTGGGAAG CATCCTGTCT GGCCAGAGCC TCCTGATGAT GAGCAGTGCT 
GATGTTGTCA TACACCGGGA TGGCTCTCTC AGCGCCAAGA GGGCAGCTCC TGTTTCTCTT 
CAGCGAAATT CTGTGACTCA ATCCAGAGAA GAGTCCAGGC TCAGAGACAA CCCGCAGCCT 
GGGGCACTAC CCTCAGAGAG TGCATCTGGT GGATTCGTGG GAGACAGACA ACCAAACTCA 
GGGCTGAGCT GTGGGAATAG AACAGCTCTG TGCTGTCTCC CTGCCCGCAT TGCCCAGACA 
CCTGTGAGGT CAGATCCCTC ACTGACTCCT CGCTCAGGTC TGTCTAGGAC CCTTTCAGAT 
GAGAACAGGC CCAGCCGGAC ACACAGCAGC AGCCCCCAAC TCAATGGCTC CAATGTACGG 
GTTAGTTCTG CCTCGACTAA GATCGTGACT CATTCTAGCT TTCCATCGAA AAATACAGCT 
TCTGGGCTTC CTCAGAGAAC AGGTCCCAGG AGACCTGATT TCTCCAAGCT ACCCAGGATA 
CCAAAGATCC ATCGGGATGG CAATAAGAGC ACGCAGGACC AGGCCCCAGC CAGCGGCCAC 
ATTGTCGAGC TCCCTAGCAC TTGCATCAGC CGCCTGACTG GCAGGGAAGG CCCTGGGCAG 
CCAGGACGAG GCCGAGTCGA CAGTGAGCCA AGCAGCAGGG GTCCCCAGGA GACTGGCTCA 
CACACAAGTG GTTCTCGACC TCCTGCGCCC AGCTCCCATG GCAGCTTAGC CCATCTAGGG 
CCCTCAAGAG GAAAGGGCAT TGGGTCTAGC TTTGAAAGTT TCCGGATCAA CATCCCTGGG 
AACACTGCAC ATTGCAGCCA GCTGTCCAGC CCTGGATTCT GTAACACGTT CCGGCCGGTA 
GACAGCAAGG TGCAGAGGAA GGAGACCCCG TTCCCCCTCT TCTCCATCAA GAAGCCGAAA 
CAACTCAAAA GCGAAATCTA TGACCCCTTT GACCCCACTG GCTCGGACTC TAGCCCTCCT 
AGCAGCAGCC CAGAGAGTCT TGGTCCAGGC CTCCTACCCT CTGAGATCAC AAGAACTATC 
TCCATCAACA GCCCAAAGGC CCCAGCCTTC CAGACTGTGC GCTGTGTTAC CTCCTACAGG 
GTAGAAAGCA TCTTTGGGAC TGAGATGGAA CCTGAGCCCC AGCCCCCTAG TGAGCCTGTA 
TCTGGCATGC TGGAGTTACT CAGCAAGGGA TCTGCTGAGG GAACCTCTGA TTTGGAGCAA 
GAGGGACTAG GGGAAATAGA GCCTACAGAG ATTCGGGGCT CCACAGCCCG GACACAGAGG 
CCACCCCCAC CAGACCCTTG GGATGATGAG GACGAAGTGT CCTGTACACC CTTCTTTGGC 
TCTGAGGAAC GGACAGTGAC GTGTGTGACT GTTGAGGAGC CAGGTGTTCT ACCAAGCCCA 
GATGCTCCAC AGATCACCAC CCACAGAATT GTGGAGTTCA GGGCCTCATC CCGTTCCCGC 
TCCACCTCCA GCTCCCGAAG CAGGAAGAAA ACGAAGAAGA AGAAGAAAAA GGTTGCCAGA 
GAGCACCAGA GGACACGCTC CAGCACTCGC TCTGGCTCCA GGGACAGGAC TTCACGCTCA 
GTATCTCCAG TTGCTGAGGA ACACACCAGG AGGCACAGAG CCAAGACTAA GAGCCGGAGG 
TCTTCCAGTG ACCGTGCCAG CAGCCAGGAC AGAGCAAAGA GGAGGAAGGA CAGAGACGAC 
AGAGACAGGG AGCATAGGCG GGGTTCCTGG GGTCATGGCA GGTGCCGGCG GAAGTCCAGG 
TCTCGCTCAG GGAGCCCTGG CAGTTCTTCC TGTGAGCGCC ATGAGAGTAA GAGACGGAAG 
AGGCGACATT CGGGGTCCAG GTCTCGGGGC AGCAGTCTGG AGAGGGACCG GAGACACAAA 
CATCGGGAGA GGAGCCGAGA GCGAATGGAC AAGCAGGAGA GTGTGACCCG GTCCCGGGAG 
AGGAGGAGGT GGCGATCTCG GTCGCCTAGC TTGGAGCATA GGCCACGAAG GCCACCTTCC 
CGTGAGAAGC GTGCCCATTC CCCAGAGAAA AAGGGGCCTG TGAGGGAGGT TTCCCCAGCC 
CCTGCTACAC AGGGGGAGTC GAGGCAGGAT GGAGACCACT CTGCTGAGCC TCCGGTTTCA 
GAAGTAAGTG TCTTGCCTGA GGTAGTAAGT GTCCTGCCAG AGGTGGTTGT GGCTGACCTG 
AACCCTCCAG AAGTCCCTCC TGTCCTGGCA GAGCCAGTTG CCCATGTGCC TGAGGACCTG 
GACTATGGTG AGTCTGTGGA GGCAGGCCAC GTCTTTGAGG ATTTTTCAAA TGAAGCCATC 
TTCATCCAGC TCGATGACAT GAGCTCGCCT CCTTCTCCTG AGAGTACAGA CTCCTCCCCT 
GAGAGAGACT TCCCACCAAA TCCCATACTG CCCCCAGCCA GCCTTCCACA AGACAGTACT 
TTACCTACCA TCCAAAGGGA GGTGTTACCC ATTCACAGTG AAGATATCTC AAAGCCTGTG 
CCCCAGGCCT TGGCCCCATC AGACCAGAGT CTGCTCAAGC AAGACACTGT AGAGATCACC 
ACTACAACTC CCAGCACCCC AGCTGTGGTG CCCATGACAA AGGACAGTCC GGTGCTGAGT 
GCAAGAGGAT GGGAAGCAGT CAGGCCCAGG GATGCTGTGG CCCAGGCCCC ACTGCTACGA 
TCCCGAACCC TGGTGAAAAG AGTCACCTGG AACCTGCAGG AAGCAGAACA CAGCACGCCA 
GCAGCCCTGG ACAGAGATCC AAGGACACCA CTTCAGAGGC CCCAGAGGCC CCAGGAAGGA 
GACTGGGATG CAGAGGACAG GGCCCTCATA GGATTTCAGC AGGCACCATT CTCCGAGCTG 
CCCCCTCCCA TCCACGTGCT CCAGGAGTCT GGGTTACCTG ATGCAGATCC CTCTCAGGTT 
TACAGCCCCA ACATGCCACC ACCCTTGGCT CAGCCCTCAA GCATCCTGCC CTATGCACTG 
GTCAGCCAGC CCTCAGTCCA GTTGATCCTG CAGGGGACCC TTCCCCTAGC AGGCTGCGGT 
ACAGCACAGA GCCTGGCACC AGTGCCCACA ATGCCAGCTA CAGTCTCAGA GCTAGCTGTT 
CCAACCACCA ACAACTCAGA AGAAAGGACT GCTACTCCCA AAACAGCTGC TGAGAAGACC 
AAAAAGGAGG AGTACATGAA GAAGCTGCAC ATGCAAGAAC GGGCTGTGGA GGAGGTGAAG 
CTGGCCATTA AACCCTTCTA CCAGAAGAGA GAAGTGACCA AGGAGGAGTA CAAGGACATA 
CTGCGCAAAG CCGTGCAGAA GATCTGCCAC AGCAAGAGTG GTGAAATCAA CCCTGTAAAG 
GTGGCCAACC TGGTGAAGGC CTATGTGGAC AAATACAGGC ATATGCGCAG ACACAAGAAG 
ACTGAAGGTG GAGAGGAGCC ACCCACTCAG GGTGCTGAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Phrf1
Entrez Gene ID	101471
Full Name	PHD and ring finger domains 1
Synonyms	AA673488
General protein information	Preferred Names PHD and ring finger domains 1 Names PHD and RING finger domain-containing protein 1 phrf1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F5

Pan troglodytes (chimpanzee)	PHRF1	XP_508199.3
Mus musculus (house mouse)	Phrf1	NP_001074587.1
Homo sapiens (human)	PHRF1	NP_001273510.1
	PHRF1	XP_001086134.2
Canis lupus familiaris (dog)	PHRF1	XP_540525.3
Rattus norvegicus (Norway rat)	Phrf1	NP_620793.1
Gallus gallus (chicken)	PHRF1	XP_004941520.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Phrf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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