SPTA1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following SPTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc60362 XM_009435126.2
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OPc60363 XM_016929916.1
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OPc60364 XM_016929927.1
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc93115 XM_009435126.3
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OPc93116 XM_016929916.2
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OPc93117 XM_016929927.2
Latest version!
Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc60362
    Clone ID Related Accession (Same CDS sequence) XM_009435126.2
    Accession Version XM_009435126.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009435126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAG CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGCAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACACCAAAGG TGTGAGTGAA GAGACCCTAA AGGAGTTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCGGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTGCTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009433401.2
    CDS96..7355
    Translation

    Target ORF information:

    RefSeq Version XM_009435126.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009435126.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAG CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGCAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACACCAAAGG TGTGAGTGAA GAGACCCTAA AGGAGTTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCGGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTGCTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc60363
    Clone ID Related Accession (Same CDS sequence) XM_016929916.1
    Accession Version XM_016929916.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAG CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGCAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACACCAAA
    GGTGTGAGTG AAGAGACCCT AAAGGAGTTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCGGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTGC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016785405.1
    CDS133..7374
    Translation

    Target ORF information:

    RefSeq Version XM_016929916.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016929916.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAG CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGCAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACACCAAA
    GGTGTGAGTG AAGAGACCCT AAAGGAGTTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCGGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTGC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc60364
    Clone ID Related Accession (Same CDS sequence) XM_016929927.1
    Accession Version XM_016929927.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAACGAGA AGACTGCTGC GATCAATGCT GATGAGCTGC CAACAGATGT GGCTGGTGGA 
    GAAGTTCTAC TGGACAGGCA TCAGCAGCAT AAGCATGAGA TTGACTCTTA CGATGACCGA
    TTTGAATCTG CTCGTGAGAC TGGTCAAGAC CTCCTGAATG CCAATCATGA AGCCTCCGAT
    GAAGTTCGGG AAAAGATGGA AATACTTACC AAAAACTGGG CTGCCCTGCT GGAACTGTGG
    AACAAGCGTC ATCATCAGTA TGAGCAGTGC TTGGACTTTC ATCTCTTCTA CAGAGACAGT
    GAGCAAGTGG ACAGTTGGAT GAGTAGACAA GAGGCCTTCC TGGAAAACGA GGATCTGGGA
    AACTCACTGG GCAGTGCAGA AGCCCTTCTT CAGAAGCATG AAGACTTTGA GGAAGCCTTT
    ACTGCCCAGG AAGAGAAGAT CACAACTGTA GACAAGACTG CAACCAAATT GATTGGTGAT
    AACCATTATG ATTCAGACAA CATCGAGGCT ATCCGTGACG GGCTGTTAGC CCGGCGGGAT
    GCCCTACGTG AAAAGGCTGC CACTAGACAT AGATTGCTGA AGGAGTCATT GCTTCTGCAA
    AAACTGTATG AGGACTCAGA TGACCTAAAG AACTGGATCA ACAAGAAGAA AAAGTTGGCA
    GATGATGAAG ATTACAAGGA CATACAGAAC TTGAAGAGTA GGGTTCAAAA GCAGCAAGTC
    TTTGAAAAGG AGTTGGAAGT TAATAAGACC CTGCTGGAAA ACATACAGAA AACTGGCCAA
    GAGATGATTG AGGGTGGTCA CTATGCCTCT GACAATGTGA CCACTCGTCT GAGTGAAGTT
    GTCAGCCTCT GGAAGGAGTT GATGGAGGCT ACAGAACAGA AAGGGACCCA GTTGCATGAG
    GCCAACCAGC AGCTGCAATT TGAAAATAAT GCAGAAGATT TGCAGCACTG GCTGGAGGAT
    GTTGAGTGGC AAGTCACCTC TGAGGATTAT GGGAAAGGCC TGGCCGATGT ACAGAATCGA
    CTCAGGAAAC ACGGCCTCCT GGAGTCGGCT GTGGCTGCTC GTCAGGATCA GGTGGATACC
    CTTACAGACC TGGCTGCATA TTTTGAAGAA ATAGGCCATC CTGATTCTAA GGATATAAGG
    GCAAGGCAGG AGTCGTTGGT ATGCCGATTT GAAGCTCTGA AAGAGCCACT GGCCACCCGA
    AAGAAGAATC TCTTAGACCG TCTCCATCTG CAGCTAATTT GTAGAGACAC AGAGGATGAG
    GAGGCCTGGA TCCAAGAGAC TGAACCCTCA GCTACTTCCA CCTACCTTGG AAAGGACCTG
    ATTGCTTCCA AAAAGCTTCT GAATAGGCAC AGAGTCATCC TGGAGAACAT TGCCAGCCAT
    GAACCACGCA TTCAAGAGAT AACAGAAAGG GGAAACAAGA TGGTAGAGGA AGGACACTTT
    GCTGCAGAAG ATGTGGCCTC TAGGGTCAAG AGTTTGAACC AGAATATGGA GTCTCTCTGT
    GCTCGAGCTG CTAGGCGACA AAATAATCTT GAAGCCAATG TCCAGTTCCA GCAGTACCTG
    GCTGACCTGC ATGAAGCAGA AACATGGATC AGAGAGAAGG AGCCTATTGT AGATAATACT
    AACTATGGGG CTGATGAAGA AGCAGCTGGG GCTCTTCTAA AGAAGCATGA GGCCTTTCTA
    TTAGATCTCA ATTCATTTGG AGACAGTATG AAAGCTCTAC GGAATCAGGC AAACGCCTGC
    CAGCAACAAC AGGCTGCACC AGTGGAGGGA GCTGCTGGAG AACAAAGGGT CATGGCTTTA
    TATGACTTCC AGGCCCGCAG CCCCCGAGAA GTCACCATGA AGAAAGGTGA TGTCTTAACG
    CTGCTCAGTT CCATCAATAA GGACTGGTGG AAGGTGGAAG CTGCTGATCA TCAGGGCATT
    GTCCCAGCTG TCTATGTCAG AAGACTGGCC CACGATGAGT TCCCGATGCT CCCACAGCGG
    CGACGAGAAG AGCCAGGCAA CATCACCCAG CGCCAGGAGC AGATTGAGAA CCAATACCGC
    TCCCTCTTGG ATCGGGCAGA AGAACGCAGG CGTCGTCTAT TGCAACGTTA TAATGAATTT
    TTATTGGCCT ATGAGGCAGG AGACATGCTG GAATGGATTC AAGAGAAAAA GGCAGAAAAC
    ACTGGAGTGG AACTAGATGA TGTTTGGGAG CTGCAGAAAA AGTTTGATGA GTTCCAAAAG
    GATTTGAATA CCAATGAGCC TCGGCTAAGG GATATCAACA AGGTAGCTGA TGATCTACTG
    TTTGAAGGAC TTCTAACACC AGAAGGAGCT CAAATCCGGC AGGAATTGAA TGCCCGCTGG
    GGTTCTTTGC AGAGGCTTGC AGATGAACAG CGGCAGCTGC TGGGCAGTGC CCATGCTGTT
    GAAGTGTTTC ACAGAGAAGC AGATGACACG AAGGAGCAGA TTGAGAAGAA ATGCCAGGCC
    CTCAGTGCTG CAGACCCTGG CTCAGATCTG TTCAGTGTTC AGGCTCTTCA GCGACGGCAT
    GAGGGCTTTG AAAGGGACCT CGTACCCCTG GGAGATAAGG TGACCATACT GGGGGAGACA
    GCAGAGCGGC TCAGTGAGTC CCATCCAGAT GCCACTGAGG ACCTGCAGAG ACAGAAAATG
    GAGCTGAATG AGGCCTGGGA AGACCTGCAG GGACGTACAA AGGATCGTAA GGAGAGCCTA
    AATGAGGCCC AGAAATTCTA CCTGTTCCTC AGCAAGGCCA GGGATCTGCA GAACTGGATC
    AGTAACATTG GTGGCATGGT ATCATCACAG GAGCTGGCCG AAGACTTAAC TGGCATAGAG
    ATCTTGCTGG AGAGACATCA GGAGCACCAT GCTGACATGG AGGCAAAGGC TCCCACCTTC
    CAGGCCTTAG AGGACTTCAG TGCAGAACTT ATCGACAGTG GGCACCATGC TAGCCCTGAA
    ATTGAAAAAA AGCTTCAAGC TGTCAAGCTA GAGAGAGATG ATTTGGAGAA GGCTTGGGAA
    CAACGCAAGA AGATCCTAGA CCAGTGCCTG GAGTTGCAGA TGTTCCAGGG GAACTGTGAT
    CAAGTTGAGA GCTGGATGGT GGCACGTGAG AATTCCCTGA GGTCAGATGA CAAAGGTTCC
    TTAGACAGTC TGGAGGCCTT GATGAAGAAA CGGGAAGATT TGGACAAAGC AATCACTGCC
    CAGGAAGGGA AGATCACTGA CCTAGAACAT TTTGCTGAGA GCCTCATTGC TGATGAACAC
    TATGCCAAAG AAGAGATTGC TACTCGGCTG CAACGTGTAC TAGACAGGTG GAAGGCTCTC
    AAAGCACAAC TGATTGATGA GCGGACAAAG CTTGGAGACT ATGCCGACCT AAAACAATTC
    TACCAAAACC TTGAGGAGCT GGAAGAATGG ATCAGTGAGA TGCTGCCCAC AGCCTGTGAT
    GAATCCTACA AAGACCCCAC TAACATTCAG AGGAAATACC TGAAACACCA GACCTTTGCA
    AATGAAGTCG ATGGCCGATC TAAGCAGGTG CATGACGTCA TCAACCTGGG GAACTCCCTG
    ATTGAGCGTA GCGCTTGTGA TGGCAATGAA GAGGCCATGC AGGAGCAACT GGAACGGCTG
    AAGGAACATT GGGATCATCT GCTTGAGAGA ACAAATGACA AAGGGCAGAA GCTCAATGAG
    GCCAGTCGTC AGCAGAGGTT CAACACAAGC ATCCGGGACT TTGAGTTCTG GCTCTCAGAG
    GCAGAGACAT TGCTGGCCAT GAAAGATCAG GCCAGGGACT TGGCTTCAGC AGGAAACCTA
    CTCAAGAAGC ATCAGCTATT GGAGACAGAG ATGTTGGCTC GAGAGGATGC ACTCAAGGAC
    CTGAATACAT TGGCTGAAGA TTTGCTCTCC AGCGGGACTT TCAACGTTGA TCAGATTATG
    GAGAAAAAAG ATAATGTCAA CAAGCGTTTC CTGAATGTCC AAGAATTGGC AGCTGCACAC
    CATGAAAAAT TGAAAGAGGC CTATGCCTTG TTCCAGTTCT TCCAGGATCT AGATGATGAG
    GAATCCTGGA TAGAGGAGAA GTTGATACGA GTGAGCTCCC AGGACTATGG GAGAGATCTT
    CAGGGCGTTC AGAACTTGCT GAAGAAGCAC AAACGCCTAG AGGGGGAGCT GGTGGCACAT
    GAGCCTGCCA TCCAGAATGT GCTGGATATG GCAGAGAAGC TGAAAGACAA GGCTGCTGTG
    GGGCAAGAGG AGATCCAGTT GCGGCTGGCT CAGTTTGTTG AACACTGGGA GAAGCTCAAA
    GAGTTGGCCA AGGCCCGAGG ACTTAAGTTG GAAGAATCCC TAGAATACTT GCAATTCATG
    CAGAATGCTG AGGAAGAGGA AGCTTGGATC AATGAAAAGA ATGCTTTGGC TGTCCGAGGA
    GATTCTGGAG ATACATTAGC TGCTACTCAG AGCTTGCTAA TGAAGCATGA AGCTTTGGAA
    AATGACTTTG CTGTCCATGA GACCCGAGTA CAAAATGTGT GTGCACAAGG AGAAGACATC
    CTAAATAAGG TGTTGCAGGA GGAAAGTCAG AACAAAGAGA TTTCTTCCAA GATAGAGGCT
    CTGAATGAAA AGACCCCTTC TCTGGCCAAG GCAATAGCTG CTTGGAAGTT GCAATTGGAA
    GACGATTATG CCTTTCAGGA ATTCAACTGG AAGGCTGATG TGGTAGAGGC TTGGATAGCT
    GATAAGGAAA CAAGCCTAAA GACCAATGGC AATGGTGCAG ACCTTGGTGA CTTCCTCACT
    CTCCTGGCGA AACAGGACAC TCTGGATGCC AGTCTGCAGA GTTTCCAGCA AGAGAGACTT
    CCCGAGATCA CCGACCTGAA GGACAAACTG ATTTCTGCTC AACACAACCA GTCTAAAGCC
    ATTGAAGAGC GTTATGCCGC TCTGCTGAAG CGCTGGGAAC AGTTGCTGGA AGCCTCGGCA
    GTCCACAGAC AGAAATTGCT GGAGAAACAG CTGCCTCTAC AGAAGGCTGA GGACCTGTTC
    GTGGAATTTG CACATAAGGC TTCAGCTTTG AACAACTGGT GTGAAAAGAT GGAAGAAAAC
    TTGTCAGAGC CTGTGCACTG TGTCTCCCTG AATGAAATTC GGCAGCTGCA GAAAGACCAT
    GAGGACTTCT TGGCCTCCCT GGCTGGGGCT CAAGCAGACT TTAAATGTTT GCTGGAGCTA
    GACCAGCAGA TTAAGGCCTT AGGTGTGCCT TCCAGCCCTT ATACCTGGTT AACAGTGGAG
    GTGCTGGAAA GGACCTGGAA GCACCTATCT GACATCATTG AGGAACGGGA GCAGGAGCTG
    CAAAAGGAAG AGGCAAGACA GGTCAAGAAC TTTGAGATGT GTCAGGAGTT TGAACAGAAT
    GCCAGTACCT TCCTTCAATG GATCCTGGAA ACCAGGGCTT ACTTTCTGGA TGGATCATTG
    CTCAAAGAAA CAGGAACTCT GGAATCTCAG CTGGAAGCAA ATAAAAGAAA ACAGAAGGAG
    ATCCAGGCGA TGAAGCGTCA ACTAACCAAG ATTGTGGACC TGGGGGACAA CTTGGAAGAC
    GCTCTGATCC TTGATATCAA ATACAGCACC ATTGGATTGG CTCAGCAGTG GGACCAGCTC
    TACCAGCTTG GGTTGCGGAT GCAACACAAC CTGGAGCAAC AGATCCAGGC CAAGGACACC
    AAAGGTGTGA GTGAAGAGAC CCTAAAGGAG TTTAGCACAA TCTATAAACA CTTTGATGAG
    AATTTGACAG GGCGCCTGAC TCACAAAGAG TTCCGGTCCT GCCTGAGAGG ACTCAATTAC
    TACTTGCCCA TGGTGGAGGA GGATGAACAT GAGCCCAAGT TTGAGAAGTT CCTGGATGCT
    GTGGATCCAG GGAGGAAGGG CTATGTCTCA CTGGAGGACT ATACTGCTTT CCTGATTGAC
    AAGGAGTCAG AAAACATCAA GTCCAGTGAT GAAATAGAGA ATGCCTTCCA AGCCCTGGCG
    GAGGGCAAGT CATATATTAC CAAAGAAGAC ATGAAGCAGG CCCTTACCCC AGAGCAAGTG
    TCATTCTGTG CCACACATAT GCAGCAATAT ATGGACCCAC GGGGTCGAAG CCATCTCTCT
    GGCTATGACT ACGTTGGCTT CACCAATTCC TGCTTTGGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016785416.1
    CDS129..6233
    Translation

    Target ORF information:

    RefSeq Version XM_016929927.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016929927.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAACGAGA AGACTGCTGC GATCAATGCT GATGAGCTGC CAACAGATGT GGCTGGTGGA 
    GAAGTTCTAC TGGACAGGCA TCAGCAGCAT AAGCATGAGA TTGACTCTTA CGATGACCGA
    TTTGAATCTG CTCGTGAGAC TGGTCAAGAC CTCCTGAATG CCAATCATGA AGCCTCCGAT
    GAAGTTCGGG AAAAGATGGA AATACTTACC AAAAACTGGG CTGCCCTGCT GGAACTGTGG
    AACAAGCGTC ATCATCAGTA TGAGCAGTGC TTGGACTTTC ATCTCTTCTA CAGAGACAGT
    GAGCAAGTGG ACAGTTGGAT GAGTAGACAA GAGGCCTTCC TGGAAAACGA GGATCTGGGA
    AACTCACTGG GCAGTGCAGA AGCCCTTCTT CAGAAGCATG AAGACTTTGA GGAAGCCTTT
    ACTGCCCAGG AAGAGAAGAT CACAACTGTA GACAAGACTG CAACCAAATT GATTGGTGAT
    AACCATTATG ATTCAGACAA CATCGAGGCT ATCCGTGACG GGCTGTTAGC CCGGCGGGAT
    GCCCTACGTG AAAAGGCTGC CACTAGACAT AGATTGCTGA AGGAGTCATT GCTTCTGCAA
    AAACTGTATG AGGACTCAGA TGACCTAAAG AACTGGATCA ACAAGAAGAA AAAGTTGGCA
    GATGATGAAG ATTACAAGGA CATACAGAAC TTGAAGAGTA GGGTTCAAAA GCAGCAAGTC
    TTTGAAAAGG AGTTGGAAGT TAATAAGACC CTGCTGGAAA ACATACAGAA AACTGGCCAA
    GAGATGATTG AGGGTGGTCA CTATGCCTCT GACAATGTGA CCACTCGTCT GAGTGAAGTT
    GTCAGCCTCT GGAAGGAGTT GATGGAGGCT ACAGAACAGA AAGGGACCCA GTTGCATGAG
    GCCAACCAGC AGCTGCAATT TGAAAATAAT GCAGAAGATT TGCAGCACTG GCTGGAGGAT
    GTTGAGTGGC AAGTCACCTC TGAGGATTAT GGGAAAGGCC TGGCCGATGT ACAGAATCGA
    CTCAGGAAAC ACGGCCTCCT GGAGTCGGCT GTGGCTGCTC GTCAGGATCA GGTGGATACC
    CTTACAGACC TGGCTGCATA TTTTGAAGAA ATAGGCCATC CTGATTCTAA GGATATAAGG
    GCAAGGCAGG AGTCGTTGGT ATGCCGATTT GAAGCTCTGA AAGAGCCACT GGCCACCCGA
    AAGAAGAATC TCTTAGACCG TCTCCATCTG CAGCTAATTT GTAGAGACAC AGAGGATGAG
    GAGGCCTGGA TCCAAGAGAC TGAACCCTCA GCTACTTCCA CCTACCTTGG AAAGGACCTG
    ATTGCTTCCA AAAAGCTTCT GAATAGGCAC AGAGTCATCC TGGAGAACAT TGCCAGCCAT
    GAACCACGCA TTCAAGAGAT AACAGAAAGG GGAAACAAGA TGGTAGAGGA AGGACACTTT
    GCTGCAGAAG ATGTGGCCTC TAGGGTCAAG AGTTTGAACC AGAATATGGA GTCTCTCTGT
    GCTCGAGCTG CTAGGCGACA AAATAATCTT GAAGCCAATG TCCAGTTCCA GCAGTACCTG
    GCTGACCTGC ATGAAGCAGA AACATGGATC AGAGAGAAGG AGCCTATTGT AGATAATACT
    AACTATGGGG CTGATGAAGA AGCAGCTGGG GCTCTTCTAA AGAAGCATGA GGCCTTTCTA
    TTAGATCTCA ATTCATTTGG AGACAGTATG AAAGCTCTAC GGAATCAGGC AAACGCCTGC
    CAGCAACAAC AGGCTGCACC AGTGGAGGGA GCTGCTGGAG AACAAAGGGT CATGGCTTTA
    TATGACTTCC AGGCCCGCAG CCCCCGAGAA GTCACCATGA AGAAAGGTGA TGTCTTAACG
    CTGCTCAGTT CCATCAATAA GGACTGGTGG AAGGTGGAAG CTGCTGATCA TCAGGGCATT
    GTCCCAGCTG TCTATGTCAG AAGACTGGCC CACGATGAGT TCCCGATGCT CCCACAGCGG
    CGACGAGAAG AGCCAGGCAA CATCACCCAG CGCCAGGAGC AGATTGAGAA CCAATACCGC
    TCCCTCTTGG ATCGGGCAGA AGAACGCAGG CGTCGTCTAT TGCAACGTTA TAATGAATTT
    TTATTGGCCT ATGAGGCAGG AGACATGCTG GAATGGATTC AAGAGAAAAA GGCAGAAAAC
    ACTGGAGTGG AACTAGATGA TGTTTGGGAG CTGCAGAAAA AGTTTGATGA GTTCCAAAAG
    GATTTGAATA CCAATGAGCC TCGGCTAAGG GATATCAACA AGGTAGCTGA TGATCTACTG
    TTTGAAGGAC TTCTAACACC AGAAGGAGCT CAAATCCGGC AGGAATTGAA TGCCCGCTGG
    GGTTCTTTGC AGAGGCTTGC AGATGAACAG CGGCAGCTGC TGGGCAGTGC CCATGCTGTT
    GAAGTGTTTC ACAGAGAAGC AGATGACACG AAGGAGCAGA TTGAGAAGAA ATGCCAGGCC
    CTCAGTGCTG CAGACCCTGG CTCAGATCTG TTCAGTGTTC AGGCTCTTCA GCGACGGCAT
    GAGGGCTTTG AAAGGGACCT CGTACCCCTG GGAGATAAGG TGACCATACT GGGGGAGACA
    GCAGAGCGGC TCAGTGAGTC CCATCCAGAT GCCACTGAGG ACCTGCAGAG ACAGAAAATG
    GAGCTGAATG AGGCCTGGGA AGACCTGCAG GGACGTACAA AGGATCGTAA GGAGAGCCTA
    AATGAGGCCC AGAAATTCTA CCTGTTCCTC AGCAAGGCCA GGGATCTGCA GAACTGGATC
    AGTAACATTG GTGGCATGGT ATCATCACAG GAGCTGGCCG AAGACTTAAC TGGCATAGAG
    ATCTTGCTGG AGAGACATCA GGAGCACCAT GCTGACATGG AGGCAAAGGC TCCCACCTTC
    CAGGCCTTAG AGGACTTCAG TGCAGAACTT ATCGACAGTG GGCACCATGC TAGCCCTGAA
    ATTGAAAAAA AGCTTCAAGC TGTCAAGCTA GAGAGAGATG ATTTGGAGAA GGCTTGGGAA
    CAACGCAAGA AGATCCTAGA CCAGTGCCTG GAGTTGCAGA TGTTCCAGGG GAACTGTGAT
    CAAGTTGAGA GCTGGATGGT GGCACGTGAG AATTCCCTGA GGTCAGATGA CAAAGGTTCC
    TTAGACAGTC TGGAGGCCTT GATGAAGAAA CGGGAAGATT TGGACAAAGC AATCACTGCC
    CAGGAAGGGA AGATCACTGA CCTAGAACAT TTTGCTGAGA GCCTCATTGC TGATGAACAC
    TATGCCAAAG AAGAGATTGC TACTCGGCTG CAACGTGTAC TAGACAGGTG GAAGGCTCTC
    AAAGCACAAC TGATTGATGA GCGGACAAAG CTTGGAGACT ATGCCGACCT AAAACAATTC
    TACCAAAACC TTGAGGAGCT GGAAGAATGG ATCAGTGAGA TGCTGCCCAC AGCCTGTGAT
    GAATCCTACA AAGACCCCAC TAACATTCAG AGGAAATACC TGAAACACCA GACCTTTGCA
    AATGAAGTCG ATGGCCGATC TAAGCAGGTG CATGACGTCA TCAACCTGGG GAACTCCCTG
    ATTGAGCGTA GCGCTTGTGA TGGCAATGAA GAGGCCATGC AGGAGCAACT GGAACGGCTG
    AAGGAACATT GGGATCATCT GCTTGAGAGA ACAAATGACA AAGGGCAGAA GCTCAATGAG
    GCCAGTCGTC AGCAGAGGTT CAACACAAGC ATCCGGGACT TTGAGTTCTG GCTCTCAGAG
    GCAGAGACAT TGCTGGCCAT GAAAGATCAG GCCAGGGACT TGGCTTCAGC AGGAAACCTA
    CTCAAGAAGC ATCAGCTATT GGAGACAGAG ATGTTGGCTC GAGAGGATGC ACTCAAGGAC
    CTGAATACAT TGGCTGAAGA TTTGCTCTCC AGCGGGACTT TCAACGTTGA TCAGATTATG
    GAGAAAAAAG ATAATGTCAA CAAGCGTTTC CTGAATGTCC AAGAATTGGC AGCTGCACAC
    CATGAAAAAT TGAAAGAGGC CTATGCCTTG TTCCAGTTCT TCCAGGATCT AGATGATGAG
    GAATCCTGGA TAGAGGAGAA GTTGATACGA GTGAGCTCCC AGGACTATGG GAGAGATCTT
    CAGGGCGTTC AGAACTTGCT GAAGAAGCAC AAACGCCTAG AGGGGGAGCT GGTGGCACAT
    GAGCCTGCCA TCCAGAATGT GCTGGATATG GCAGAGAAGC TGAAAGACAA GGCTGCTGTG
    GGGCAAGAGG AGATCCAGTT GCGGCTGGCT CAGTTTGTTG AACACTGGGA GAAGCTCAAA
    GAGTTGGCCA AGGCCCGAGG ACTTAAGTTG GAAGAATCCC TAGAATACTT GCAATTCATG
    CAGAATGCTG AGGAAGAGGA AGCTTGGATC AATGAAAAGA ATGCTTTGGC TGTCCGAGGA
    GATTCTGGAG ATACATTAGC TGCTACTCAG AGCTTGCTAA TGAAGCATGA AGCTTTGGAA
    AATGACTTTG CTGTCCATGA GACCCGAGTA CAAAATGTGT GTGCACAAGG AGAAGACATC
    CTAAATAAGG TGTTGCAGGA GGAAAGTCAG AACAAAGAGA TTTCTTCCAA GATAGAGGCT
    CTGAATGAAA AGACCCCTTC TCTGGCCAAG GCAATAGCTG CTTGGAAGTT GCAATTGGAA
    GACGATTATG CCTTTCAGGA ATTCAACTGG AAGGCTGATG TGGTAGAGGC TTGGATAGCT
    GATAAGGAAA CAAGCCTAAA GACCAATGGC AATGGTGCAG ACCTTGGTGA CTTCCTCACT
    CTCCTGGCGA AACAGGACAC TCTGGATGCC AGTCTGCAGA GTTTCCAGCA AGAGAGACTT
    CCCGAGATCA CCGACCTGAA GGACAAACTG ATTTCTGCTC AACACAACCA GTCTAAAGCC
    ATTGAAGAGC GTTATGCCGC TCTGCTGAAG CGCTGGGAAC AGTTGCTGGA AGCCTCGGCA
    GTCCACAGAC AGAAATTGCT GGAGAAACAG CTGCCTCTAC AGAAGGCTGA GGACCTGTTC
    GTGGAATTTG CACATAAGGC TTCAGCTTTG AACAACTGGT GTGAAAAGAT GGAAGAAAAC
    TTGTCAGAGC CTGTGCACTG TGTCTCCCTG AATGAAATTC GGCAGCTGCA GAAAGACCAT
    GAGGACTTCT TGGCCTCCCT GGCTGGGGCT CAAGCAGACT TTAAATGTTT GCTGGAGCTA
    GACCAGCAGA TTAAGGCCTT AGGTGTGCCT TCCAGCCCTT ATACCTGGTT AACAGTGGAG
    GTGCTGGAAA GGACCTGGAA GCACCTATCT GACATCATTG AGGAACGGGA GCAGGAGCTG
    CAAAAGGAAG AGGCAAGACA GGTCAAGAAC TTTGAGATGT GTCAGGAGTT TGAACAGAAT
    GCCAGTACCT TCCTTCAATG GATCCTGGAA ACCAGGGCTT ACTTTCTGGA TGGATCATTG
    CTCAAAGAAA CAGGAACTCT GGAATCTCAG CTGGAAGCAA ATAAAAGAAA ACAGAAGGAG
    ATCCAGGCGA TGAAGCGTCA ACTAACCAAG ATTGTGGACC TGGGGGACAA CTTGGAAGAC
    GCTCTGATCC TTGATATCAA ATACAGCACC ATTGGATTGG CTCAGCAGTG GGACCAGCTC
    TACCAGCTTG GGTTGCGGAT GCAACACAAC CTGGAGCAAC AGATCCAGGC CAAGGACACC
    AAAGGTGTGA GTGAAGAGAC CCTAAAGGAG TTTAGCACAA TCTATAAACA CTTTGATGAG
    AATTTGACAG GGCGCCTGAC TCACAAAGAG TTCCGGTCCT GCCTGAGAGG ACTCAATTAC
    TACTTGCCCA TGGTGGAGGA GGATGAACAT GAGCCCAAGT TTGAGAAGTT CCTGGATGCT
    GTGGATCCAG GGAGGAAGGG CTATGTCTCA CTGGAGGACT ATACTGCTTT CCTGATTGAC
    AAGGAGTCAG AAAACATCAA GTCCAGTGAT GAAATAGAGA ATGCCTTCCA AGCCCTGGCG
    GAGGGCAAGT CATATATTAC CAAAGAAGAC ATGAAGCAGG CCCTTACCCC AGAGCAAGTG
    TCATTCTGTG CCACACATAT GCAGCAATAT ATGGACCCAC GGGGTCGAAG CCATCTCTCT
    GGCTATGACT ACGTTGGCTT CACCAATTCC TGCTTTGGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc93115
    Clone ID Related Accession (Same CDS sequence) XM_009435126.3
    Accession Version XM_009435126.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009435126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACACCAAAGG TGTGAGTGAA GAGACCCTAA AGGAGTTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCGGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTGCTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009433401.3
    CDS96..7355
    Translation

    Target ORF information:

    RefSeq Version XM_009435126.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009435126.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACACCAAAGG TGTGAGTGAA GAGACCCTAA AGGAGTTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCGGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTGCTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc93116
    Clone ID Related Accession (Same CDS sequence) XM_016929916.2
    Accession Version XM_016929916.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016929916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACACCAAA
    GGTGTGAGTG AAGAGACCCT AAAGGAGTTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCGGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTGC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016785405.2
    CDS130..7371
    Translation

    Target ORF information:

    RefSeq Version XM_016929916.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016929916.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATTTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACT
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCACATAG AGGAGCTACG CCGCCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGCCCTGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAATGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCTGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAGACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCCGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTATTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CAGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACGCTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCGCC AACTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTCTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTACTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTGA ATCTGCTCGT GAGACTGGTC AAGACCTCCT GAATGCCAAT
    CATGAAGCCT CCGATGAAGT TCGGGAAAAG ATGGAAATAC TTACCAAAAA CTGGGCTGCC
    CTGCTGGAAC TGTGGAACAA GCGTCATCAT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCACAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATAACCA TTATGATTCA GACAACATCG AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACATAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGTAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GAAGTTAATA AGACCCTGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGTCAG CCTCTGGAAG GAGTTGATGG AGGCTACAGA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CACTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GATGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATACCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAGGAGTCG TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAATCTCTTA GACCGTCTCC ATCTGCAGCT AATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCACAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAGATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCTGTGCTCG AGCTGCTAGG CGACAAAATA ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAGCCT
    ATTGTAGATA ATACTAACTA TGGGGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTACGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGCTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGCAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGGCGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTGTTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATGCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGACG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAA CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCATGCTGA CATGGAGGCA
    AAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAACAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAG GTTCCTTAGA CAGTCTGGAG GCCTTGATGA AGAAACGGGA AGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACTC GGCTGCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    GACCTAAAAC AATTCTACCA AAACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC CCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCAAATGA AGTCGATGGC CGATCTAAGC AGGTGCATGA CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GCGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC GGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    CAGAAGCTCA ATGAGGCCAG TCGTCAGCAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA CAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTATGGAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCATGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG CGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CACATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTC TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CCAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTCCT GGCGAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACCGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTG GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACACCAAA
    GGTGTGAGTG AAGAGACCCT AAAGGAGTTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCGGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTGC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc93117
    Clone ID Related Accession (Same CDS sequence) XM_016929927.2
    Accession Version XM_016929927.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6105bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product spectrin alpha chain, erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016929927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAACGAGA AGACTGCTGC GATCAATGCT GATGAGCTGC CAACAGATGT GGCTGGTGGA 
    GAAGTTCTAC TGGACAGGCA TCAGCAGCAT AAGCATGAGA TTGACTCTTA CGATGACCGA
    TTTGAATCTG CTCGTGAGAC TGGTCAAGAC CTCCTGAATG CCAATCATGA AGCCTCCGAT
    GAAGTTCGGG AAAAGATGGA AATACTTACC AAAAACTGGG CTGCCCTGCT GGAACTGTGG
    AACAAGCGTC ATCATCAGTA TGAGCAGTGC TTGGACTTTC ATCTCTTCTA CAGAGACAGT
    GAGCAAGTGG ACAGTTGGAT GAGTAGACAA GAGGCCTTCC TGGAAAACGA GGATCTGGGA
    AACTCACTGG GCAGTGCAGA AGCCCTTCTT CAGAAGCATG AAGACTTTGA GGAAGCCTTT
    ACTGCCCAGG AAGAGAAGAT CACAACTGTA GACAAGACTG CAACCAAATT GATTGGTGAT
    AACCATTATG ATTCAGACAA CATCGAGGCT ATCCGTGACG GGCTGTTAGC CCGGCGGGAT
    GCCCTACGTG AAAAGGCTGC CACTAGACAT AGATTGCTGA AGGAGTCATT GCTTCTGCAA
    AAACTGTATG AGGACTCAGA TGACCTAAAG AACTGGATCA ACAAGAAGAA AAAGTTGGCA
    GATGATGAAG ATTACAAGGA CATACAGAAC TTGAAGAGTA GGGTTCAAAA GCAGCAAGTC
    TTTGAAAAGG AGTTGGAAGT TAATAAGACC CTGCTGGAAA ACATACAGAA AACTGGCCAA
    GAGATGATTG AGGGTGGTCA CTATGCCTCT GACAATGTGA CCACTCGTCT GAGTGAAGTT
    GTCAGCCTCT GGAAGGAGTT GATGGAGGCT ACAGAACAGA AAGGGACCCA GTTGCATGAG
    GCCAACCAGC AGCTGCAATT TGAAAATAAT GCAGAAGATT TGCAGCACTG GCTGGAGGAT
    GTTGAGTGGC AAGTCACCTC TGAGGATTAT GGGAAAGGCC TGGCCGATGT ACAGAATCGA
    CTCAGGAAAC ACGGCCTCCT GGAGTCGGCT GTGGCTGCTC GTCAGGATCA GGTGGATACC
    CTTACAGACC TGGCTGCATA TTTTGAAGAA ATAGGCCATC CTGATTCTAA GGATATAAGG
    GCAAGGCAGG AGTCGTTGGT ATGCCGATTT GAAGCTCTGA AAGAGCCACT GGCCACCCGA
    AAGAAGAATC TCTTAGACCG TCTCCATCTG CAGCTAATTT GTAGAGACAC AGAGGATGAG
    GAGGCCTGGA TCCAAGAGAC TGAACCCTCA GCTACTTCCA CCTACCTTGG AAAGGACCTG
    ATTGCTTCCA AAAAGCTTCT GAATAGGCAC AGAGTCATCC TGGAGAACAT TGCCAGCCAT
    GAACCACGCA TTCAAGAGAT AACAGAAAGG GGAAACAAGA TGGTAGAGGA AGGACACTTT
    GCTGCAGAAG ATGTGGCCTC TAGGGTCAAG AGTTTGAACC AGAATATGGA GTCTCTCTGT
    GCTCGAGCTG CTAGGCGACA AAATAATCTT GAAGCCAATG TCCAGTTCCA GCAGTACCTG
    GCTGACCTGC ATGAAGCAGA AACATGGATC AGAGAGAAGG AGCCTATTGT AGATAATACT
    AACTATGGGG CTGATGAAGA AGCAGCTGGG GCTCTTCTAA AGAAGCATGA GGCCTTTCTA
    TTAGATCTCA ATTCATTTGG AGACAGTATG AAAGCTCTAC GGAATCAGGC AAACGCCTGC
    CAGCAACAAC AGGCTGCACC AGTGGAGGGA GCTGCTGGAG AACAAAGGGT CATGGCTTTA
    TATGACTTCC AGGCCCGCAG CCCCCGAGAA GTCACCATGA AGAAAGGTGA TGTCTTAACG
    CTGCTCAGTT CCATCAATAA GGACTGGTGG AAGGTGGAAG CTGCTGATCA TCAGGGCATT
    GTCCCAGCTG TCTATGTCAG AAGACTGGCC CACGATGAGT TCCCGATGCT CCCACAGCGG
    CGACGAGAAG AGCCAGGCAA CATCACCCAG CGCCAGGAGC AGATTGAGAA CCAATACCGC
    TCCCTCTTGG ATCGGGCAGA AGAACGCAGG CGTCGTCTAT TGCAACGTTA TAATGAATTT
    TTATTGGCCT ATGAGGCAGG AGACATGCTG GAATGGATTC AAGAGAAAAA GGCAGAAAAC
    ACTGGAGTGG AACTAGATGA TGTTTGGGAG CTGCAGAAAA AGTTTGATGA GTTCCAAAAG
    GATTTGAATA CCAATGAGCC TCGGCTAAGG GATATCAACA AGGTAGCTGA TGATCTACTG
    TTTGAAGGAC TTCTAACACC AGAAGGAGCT CAAATCCGGC AGGAATTGAA TGCCCGCTGG
    GGTTCTTTGC AGAGGCTTGC AGATGAACAG CGGCAGCTGC TGGGCAGTGC CCATGCTGTT
    GAAGTGTTTC ACAGAGAAGC AGATGACACG AAGGAGCAGA TTGAGAAGAA ATGCCAGGCC
    CTCAGTGCTG CAGACCCTGG CTCAGATCTG TTCAGTGTTC AGGCTCTTCA GCGACGGCAT
    GAGGGCTTTG AAAGGGACCT CGTACCCCTG GGAGATAAGG TGACCATACT GGGGGAGACA
    GCAGAGCGGC TCAGTGAGTC CCATCCAGAT GCCACTGAGG ACCTGCAGAG ACAGAAAATG
    GAGCTGAATG AGGCCTGGGA AGACCTGCAG GGACGTACAA AGGATCGTAA GGAGAGCCTA
    AATGAGGCCC AGAAATTCTA CCTGTTCCTC AGCAAGGCCA GGGATCTGCA GAACTGGATC
    AGTAACATTG GTGGCATGGT ATCATCACAG GAGCTGGCCG AAGACTTAAC TGGCATAGAG
    ATCTTGCTGG AGAGACATCA GGAGCACCAT GCTGACATGG AGGCAAAGGC TCCCACCTTC
    CAGGCCTTAG AGGACTTCAG TGCAGAACTT ATCGACAGTG GGCACCATGC TAGCCCTGAA
    ATTGAAAAAA AGCTTCAAGC TGTCAAGCTA GAGAGAGATG ATTTGGAGAA GGCTTGGGAA
    CAACGCAAGA AGATCCTAGA CCAGTGCCTG GAGTTGCAGA TGTTCCAGGG GAACTGTGAT
    CAAGTTGAGA GCTGGATGGT GGCACGTGAG AATTCCCTGA GGTCAGATGA CAAAGGTTCC
    TTAGACAGTC TGGAGGCCTT GATGAAGAAA CGGGAAGATT TGGACAAAGC AATCACTGCC
    CAGGAAGGGA AGATCACTGA CCTAGAACAT TTTGCTGAGA GCCTCATTGC TGATGAACAC
    TATGCCAAAG AAGAGATTGC TACTCGGCTG CAACGTGTAC TAGACAGGTG GAAGGCTCTC
    AAAGCACAAC TGATTGATGA GCGGACAAAG CTTGGAGACT ATGCCGACCT AAAACAATTC
    TACCAAAACC TTGAGGAGCT GGAAGAATGG ATCAGTGAGA TGCTGCCCAC AGCCTGTGAT
    GAATCCTACA AAGACCCCAC TAACATTCAG AGGAAATACC TGAAACACCA GACCTTTGCA
    AATGAAGTCG ATGGCCGATC TAAGCAGGTG CATGACGTCA TCAACCTGGG GAACTCCCTG
    ATTGAGCGTA GCGCTTGTGA TGGCAATGAA GAGGCCATGA AGGAGCAACT GGAACGGCTG
    AAGGAACATT GGGATCATCT GCTTGAGAGA ACAAATGACA AAGGGCAGAA GCTCAATGAG
    GCCAGTCGTC AGCAGAGGTT CAACACAAGC ATCCGGGACT TTGAGTTCTG GCTCTCAGAG
    GCAGAGACAT TGCTGGCCAT GAAAGATCAG GCCAGGGACT TGGCTTCAGC AGGAAACCTA
    CTCAAGAAGC ATCAGCTATT GGAGACAGAG ATGTTGGCTC GAGAGGATGC ACTCAAGGAC
    CTGAATACAT TGGCTGAAGA TTTGCTCTCC AGCGGGACTT TCAACGTTGA TCAGATTATG
    GAGAAAAAAG ATAATGTCAA CAAGCGTTTC CTGAATGTCC AAGAATTGGC AGCTGCACAC
    CATGAAAAAT TGAAAGAGGC CTATGCCTTG TTCCAGTTCT TCCAGGATCT AGATGATGAG
    GAATCCTGGA TAGAGGAGAA GTTGATACGA GTGAGCTCCC AGGACTATGG GAGAGATCTT
    CAGGGCGTTC AGAACTTGCT GAAGAAGCAC AAACGCCTAG AGGGGGAGCT GGTGGCACAT
    GAGCCTGCCA TCCAGAATGT GCTGGATATG GCAGAGAAGC TGAAAGACAA GGCTGCTGTG
    GGGCAAGAGG AGATCCAGTT GCGGCTGGCT CAGTTTGTTG AACACTGGGA GAAGCTCAAA
    GAGTTGGCCA AGGCCCGAGG ACTTAAGTTG GAAGAATCCC TAGAATACTT GCAATTCATG
    CAGAATGCTG AGGAAGAGGA AGCTTGGATC AATGAAAAGA ATGCTTTGGC TGTCCGAGGA
    GATTCTGGAG ATACATTAGC TGCTACTCAG AGCTTGCTAA TGAAGCATGA AGCTTTGGAA
    AATGACTTTG CTGTCCATGA GACCCGAGTA CAAAATGTGT GTGCACAAGG AGAAGACATC
    CTAAATAAGG TGTTGCAGGA GGAAAGTCAG AACAAAGAGA TTTCTTCCAA GATAGAGGCT
    CTGAATGAAA AGACCCCTTC TCTGGCCAAG GCAATAGCTG CTTGGAAGTT GCAATTGGAA
    GACGATTATG CCTTTCAGGA ATTCAACTGG AAGGCTGATG TGGTAGAGGC TTGGATAGCT
    GATAAGGAAA CAAGCCTAAA GACCAATGGC AATGGTGCAG ACCTTGGTGA CTTCCTCACT
    CTCCTGGCGA AACAGGACAC TCTGGATGCC AGTCTGCAGA GTTTCCAGCA AGAGAGACTT
    CCCGAGATCA CCGACCTGAA GGACAAACTG ATTTCTGCTC AACACAACCA GTCTAAAGCC
    ATTGAAGAGC GTTATGCCGC TCTGCTGAAG CGCTGGGAAC AGTTGCTGGA AGCCTCGGCA
    GTCCACAGAC AGAAATTGCT GGAGAAACAG CTGCCTCTAC AGAAGGCTGA GGACCTGTTC
    GTGGAATTTG CACATAAGGC TTCAGCTTTG AACAACTGGT GTGAAAAGAT GGAAGAAAAC
    TTGTCAGAGC CTGTGCACTG TGTCTCCCTG AATGAAATTC GGCAGCTGCA GAAAGACCAT
    GAGGACTTCT TGGCCTCCCT GGCTGGGGCT CAAGCAGACT TTAAATGTTT GCTGGAGCTA
    GACCAGCAGA TTAAGGCCTT AGGTGTGCCT TCCAGCCCTT ATACCTGGTT AACAGTGGAG
    GTGCTGGAAA GGACCTGGAA GCACCTATCT GACATCATTG AGGAACGGGA GCAGGAGCTG
    CAAAAGGAAG AGGCAAGACA GGTCAAGAAC TTTGAGATGT GTCAGGAGTT TGAACAGAAT
    GCCAGTACCT TCCTTCAATG GATCCTGGAA ACCAGGGCTT ACTTTCTGGA TGGATCATTG
    CTCAAAGAAA CAGGAACTCT GGAATCTCAG CTGGAAGCAA ATAAAAGAAA ACAGAAGGAG
    ATCCAGGCGA TGAAGCGTCA ACTAACCAAG ATTGTGGACC TGGGGGACAA CTTGGAAGAC
    GCTCTGATCC TTGATATCAA ATACAGCACC ATTGGATTGG CTCAGCAGTG GGACCAGCTC
    TACCAGCTTG GGTTGCGGAT GCAACACAAC CTGGAGCAAC AGATCCAGGC CAAGGACACC
    AAAGGTGTGA GTGAAGAGAC CCTAAAGGAG TTTAGCACAA TCTATAAACA CTTTGATGAG
    AATTTGACAG GGCGCCTGAC TCACAAAGAG TTCCGGTCCT GCCTGAGAGG ACTCAATTAC
    TACTTGCCCA TGGTGGAGGA GGATGAACAT GAGCCCAAGT TTGAGAAGTT CCTGGATGCT
    GTGGATCCAG GGAGGAAGGG CTATGTCTCA CTGGAGGACT ATACTGCTTT CCTGATTGAC
    AAGGAGTCAG AAAACATCAA GTCCAGTGAT GAAATAGAGA ATGCCTTCCA AGCCCTGGCG
    GAGGGCAAGT CATATATTAC CAAAGAAGAC ATGAAGCAGG CCCTTACCCC AGAGCAAGTG
    TCATTCTGTG CCACACATAT GCAGCAATAT ATGGACCCAC GGGGTCGAAG CCATCTCTCT
    GGCTATGACT ACGTTGGCTT CACCAATTCC TGCTTTGGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016785416.2
    CDS129..6233
    Translation

    Target ORF information:

    RefSeq Version XM_016929927.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016929927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGAACGAGA AGACTGCTGC GATCAATGCT GATGAGCTGC CAACAGATGT GGCTGGTGGA 
    GAAGTTCTAC TGGACAGGCA TCAGCAGCAT AAGCATGAGA TTGACTCTTA CGATGACCGA
    TTTGAATCTG CTCGTGAGAC TGGTCAAGAC CTCCTGAATG CCAATCATGA AGCCTCCGAT
    GAAGTTCGGG AAAAGATGGA AATACTTACC AAAAACTGGG CTGCCCTGCT GGAACTGTGG
    AACAAGCGTC ATCATCAGTA TGAGCAGTGC TTGGACTTTC ATCTCTTCTA CAGAGACAGT
    GAGCAAGTGG ACAGTTGGAT GAGTAGACAA GAGGCCTTCC TGGAAAACGA GGATCTGGGA
    AACTCACTGG GCAGTGCAGA AGCCCTTCTT CAGAAGCATG AAGACTTTGA GGAAGCCTTT
    ACTGCCCAGG AAGAGAAGAT CACAACTGTA GACAAGACTG CAACCAAATT GATTGGTGAT
    AACCATTATG ATTCAGACAA CATCGAGGCT ATCCGTGACG GGCTGTTAGC CCGGCGGGAT
    GCCCTACGTG AAAAGGCTGC CACTAGACAT AGATTGCTGA AGGAGTCATT GCTTCTGCAA
    AAACTGTATG AGGACTCAGA TGACCTAAAG AACTGGATCA ACAAGAAGAA AAAGTTGGCA
    GATGATGAAG ATTACAAGGA CATACAGAAC TTGAAGAGTA GGGTTCAAAA GCAGCAAGTC
    TTTGAAAAGG AGTTGGAAGT TAATAAGACC CTGCTGGAAA ACATACAGAA AACTGGCCAA
    GAGATGATTG AGGGTGGTCA CTATGCCTCT GACAATGTGA CCACTCGTCT GAGTGAAGTT
    GTCAGCCTCT GGAAGGAGTT GATGGAGGCT ACAGAACAGA AAGGGACCCA GTTGCATGAG
    GCCAACCAGC AGCTGCAATT TGAAAATAAT GCAGAAGATT TGCAGCACTG GCTGGAGGAT
    GTTGAGTGGC AAGTCACCTC TGAGGATTAT GGGAAAGGCC TGGCCGATGT ACAGAATCGA
    CTCAGGAAAC ACGGCCTCCT GGAGTCGGCT GTGGCTGCTC GTCAGGATCA GGTGGATACC
    CTTACAGACC TGGCTGCATA TTTTGAAGAA ATAGGCCATC CTGATTCTAA GGATATAAGG
    GCAAGGCAGG AGTCGTTGGT ATGCCGATTT GAAGCTCTGA AAGAGCCACT GGCCACCCGA
    AAGAAGAATC TCTTAGACCG TCTCCATCTG CAGCTAATTT GTAGAGACAC AGAGGATGAG
    GAGGCCTGGA TCCAAGAGAC TGAACCCTCA GCTACTTCCA CCTACCTTGG AAAGGACCTG
    ATTGCTTCCA AAAAGCTTCT GAATAGGCAC AGAGTCATCC TGGAGAACAT TGCCAGCCAT
    GAACCACGCA TTCAAGAGAT AACAGAAAGG GGAAACAAGA TGGTAGAGGA AGGACACTTT
    GCTGCAGAAG ATGTGGCCTC TAGGGTCAAG AGTTTGAACC AGAATATGGA GTCTCTCTGT
    GCTCGAGCTG CTAGGCGACA AAATAATCTT GAAGCCAATG TCCAGTTCCA GCAGTACCTG
    GCTGACCTGC ATGAAGCAGA AACATGGATC AGAGAGAAGG AGCCTATTGT AGATAATACT
    AACTATGGGG CTGATGAAGA AGCAGCTGGG GCTCTTCTAA AGAAGCATGA GGCCTTTCTA
    TTAGATCTCA ATTCATTTGG AGACAGTATG AAAGCTCTAC GGAATCAGGC AAACGCCTGC
    CAGCAACAAC AGGCTGCACC AGTGGAGGGA GCTGCTGGAG AACAAAGGGT CATGGCTTTA
    TATGACTTCC AGGCCCGCAG CCCCCGAGAA GTCACCATGA AGAAAGGTGA TGTCTTAACG
    CTGCTCAGTT CCATCAATAA GGACTGGTGG AAGGTGGAAG CTGCTGATCA TCAGGGCATT
    GTCCCAGCTG TCTATGTCAG AAGACTGGCC CACGATGAGT TCCCGATGCT CCCACAGCGG
    CGACGAGAAG AGCCAGGCAA CATCACCCAG CGCCAGGAGC AGATTGAGAA CCAATACCGC
    TCCCTCTTGG ATCGGGCAGA AGAACGCAGG CGTCGTCTAT TGCAACGTTA TAATGAATTT
    TTATTGGCCT ATGAGGCAGG AGACATGCTG GAATGGATTC AAGAGAAAAA GGCAGAAAAC
    ACTGGAGTGG AACTAGATGA TGTTTGGGAG CTGCAGAAAA AGTTTGATGA GTTCCAAAAG
    GATTTGAATA CCAATGAGCC TCGGCTAAGG GATATCAACA AGGTAGCTGA TGATCTACTG
    TTTGAAGGAC TTCTAACACC AGAAGGAGCT CAAATCCGGC AGGAATTGAA TGCCCGCTGG
    GGTTCTTTGC AGAGGCTTGC AGATGAACAG CGGCAGCTGC TGGGCAGTGC CCATGCTGTT
    GAAGTGTTTC ACAGAGAAGC AGATGACACG AAGGAGCAGA TTGAGAAGAA ATGCCAGGCC
    CTCAGTGCTG CAGACCCTGG CTCAGATCTG TTCAGTGTTC AGGCTCTTCA GCGACGGCAT
    GAGGGCTTTG AAAGGGACCT CGTACCCCTG GGAGATAAGG TGACCATACT GGGGGAGACA
    GCAGAGCGGC TCAGTGAGTC CCATCCAGAT GCCACTGAGG ACCTGCAGAG ACAGAAAATG
    GAGCTGAATG AGGCCTGGGA AGACCTGCAG GGACGTACAA AGGATCGTAA GGAGAGCCTA
    AATGAGGCCC AGAAATTCTA CCTGTTCCTC AGCAAGGCCA GGGATCTGCA GAACTGGATC
    AGTAACATTG GTGGCATGGT ATCATCACAG GAGCTGGCCG AAGACTTAAC TGGCATAGAG
    ATCTTGCTGG AGAGACATCA GGAGCACCAT GCTGACATGG AGGCAAAGGC TCCCACCTTC
    CAGGCCTTAG AGGACTTCAG TGCAGAACTT ATCGACAGTG GGCACCATGC TAGCCCTGAA
    ATTGAAAAAA AGCTTCAAGC TGTCAAGCTA GAGAGAGATG ATTTGGAGAA GGCTTGGGAA
    CAACGCAAGA AGATCCTAGA CCAGTGCCTG GAGTTGCAGA TGTTCCAGGG GAACTGTGAT
    CAAGTTGAGA GCTGGATGGT GGCACGTGAG AATTCCCTGA GGTCAGATGA CAAAGGTTCC
    TTAGACAGTC TGGAGGCCTT GATGAAGAAA CGGGAAGATT TGGACAAAGC AATCACTGCC
    CAGGAAGGGA AGATCACTGA CCTAGAACAT TTTGCTGAGA GCCTCATTGC TGATGAACAC
    TATGCCAAAG AAGAGATTGC TACTCGGCTG CAACGTGTAC TAGACAGGTG GAAGGCTCTC
    AAAGCACAAC TGATTGATGA GCGGACAAAG CTTGGAGACT ATGCCGACCT AAAACAATTC
    TACCAAAACC TTGAGGAGCT GGAAGAATGG ATCAGTGAGA TGCTGCCCAC AGCCTGTGAT
    GAATCCTACA AAGACCCCAC TAACATTCAG AGGAAATACC TGAAACACCA GACCTTTGCA
    AATGAAGTCG ATGGCCGATC TAAGCAGGTG CATGACGTCA TCAACCTGGG GAACTCCCTG
    ATTGAGCGTA GCGCTTGTGA TGGCAATGAA GAGGCCATGA AGGAGCAACT GGAACGGCTG
    AAGGAACATT GGGATCATCT GCTTGAGAGA ACAAATGACA AAGGGCAGAA GCTCAATGAG
    GCCAGTCGTC AGCAGAGGTT CAACACAAGC ATCCGGGACT TTGAGTTCTG GCTCTCAGAG
    GCAGAGACAT TGCTGGCCAT GAAAGATCAG GCCAGGGACT TGGCTTCAGC AGGAAACCTA
    CTCAAGAAGC ATCAGCTATT GGAGACAGAG ATGTTGGCTC GAGAGGATGC ACTCAAGGAC
    CTGAATACAT TGGCTGAAGA TTTGCTCTCC AGCGGGACTT TCAACGTTGA TCAGATTATG
    GAGAAAAAAG ATAATGTCAA CAAGCGTTTC CTGAATGTCC AAGAATTGGC AGCTGCACAC
    CATGAAAAAT TGAAAGAGGC CTATGCCTTG TTCCAGTTCT TCCAGGATCT AGATGATGAG
    GAATCCTGGA TAGAGGAGAA GTTGATACGA GTGAGCTCCC AGGACTATGG GAGAGATCTT
    CAGGGCGTTC AGAACTTGCT GAAGAAGCAC AAACGCCTAG AGGGGGAGCT GGTGGCACAT
    GAGCCTGCCA TCCAGAATGT GCTGGATATG GCAGAGAAGC TGAAAGACAA GGCTGCTGTG
    GGGCAAGAGG AGATCCAGTT GCGGCTGGCT CAGTTTGTTG AACACTGGGA GAAGCTCAAA
    GAGTTGGCCA AGGCCCGAGG ACTTAAGTTG GAAGAATCCC TAGAATACTT GCAATTCATG
    CAGAATGCTG AGGAAGAGGA AGCTTGGATC AATGAAAAGA ATGCTTTGGC TGTCCGAGGA
    GATTCTGGAG ATACATTAGC TGCTACTCAG AGCTTGCTAA TGAAGCATGA AGCTTTGGAA
    AATGACTTTG CTGTCCATGA GACCCGAGTA CAAAATGTGT GTGCACAAGG AGAAGACATC
    CTAAATAAGG TGTTGCAGGA GGAAAGTCAG AACAAAGAGA TTTCTTCCAA GATAGAGGCT
    CTGAATGAAA AGACCCCTTC TCTGGCCAAG GCAATAGCTG CTTGGAAGTT GCAATTGGAA
    GACGATTATG CCTTTCAGGA ATTCAACTGG AAGGCTGATG TGGTAGAGGC TTGGATAGCT
    GATAAGGAAA CAAGCCTAAA GACCAATGGC AATGGTGCAG ACCTTGGTGA CTTCCTCACT
    CTCCTGGCGA AACAGGACAC TCTGGATGCC AGTCTGCAGA GTTTCCAGCA AGAGAGACTT
    CCCGAGATCA CCGACCTGAA GGACAAACTG ATTTCTGCTC AACACAACCA GTCTAAAGCC
    ATTGAAGAGC GTTATGCCGC TCTGCTGAAG CGCTGGGAAC AGTTGCTGGA AGCCTCGGCA
    GTCCACAGAC AGAAATTGCT GGAGAAACAG CTGCCTCTAC AGAAGGCTGA GGACCTGTTC
    GTGGAATTTG CACATAAGGC TTCAGCTTTG AACAACTGGT GTGAAAAGAT GGAAGAAAAC
    TTGTCAGAGC CTGTGCACTG TGTCTCCCTG AATGAAATTC GGCAGCTGCA GAAAGACCAT
    GAGGACTTCT TGGCCTCCCT GGCTGGGGCT CAAGCAGACT TTAAATGTTT GCTGGAGCTA
    GACCAGCAGA TTAAGGCCTT AGGTGTGCCT TCCAGCCCTT ATACCTGGTT AACAGTGGAG
    GTGCTGGAAA GGACCTGGAA GCACCTATCT GACATCATTG AGGAACGGGA GCAGGAGCTG
    CAAAAGGAAG AGGCAAGACA GGTCAAGAAC TTTGAGATGT GTCAGGAGTT TGAACAGAAT
    GCCAGTACCT TCCTTCAATG GATCCTGGAA ACCAGGGCTT ACTTTCTGGA TGGATCATTG
    CTCAAAGAAA CAGGAACTCT GGAATCTCAG CTGGAAGCAA ATAAAAGAAA ACAGAAGGAG
    ATCCAGGCGA TGAAGCGTCA ACTAACCAAG ATTGTGGACC TGGGGGACAA CTTGGAAGAC
    GCTCTGATCC TTGATATCAA ATACAGCACC ATTGGATTGG CTCAGCAGTG GGACCAGCTC
    TACCAGCTTG GGTTGCGGAT GCAACACAAC CTGGAGCAAC AGATCCAGGC CAAGGACACC
    AAAGGTGTGA GTGAAGAGAC CCTAAAGGAG TTTAGCACAA TCTATAAACA CTTTGATGAG
    AATTTGACAG GGCGCCTGAC TCACAAAGAG TTCCGGTCCT GCCTGAGAGG ACTCAATTAC
    TACTTGCCCA TGGTGGAGGA GGATGAACAT GAGCCCAAGT TTGAGAAGTT CCTGGATGCT
    GTGGATCCAG GGAGGAAGGG CTATGTCTCA CTGGAGGACT ATACTGCTTT CCTGATTGAC
    AAGGAGTCAG AAAACATCAA GTCCAGTGAT GAAATAGAGA ATGCCTTCCA AGCCCTGGCG
    GAGGGCAAGT CATATATTAC CAAAGAAGAC ATGAAGCAGG CCCTTACCCC AGAGCAAGTG
    TCATTCTGTG CCACACATAT GCAGCAATAT ATGGACCCAC GGGGTCGAAG CCATCTCTCT
    GGCTATGACT ACGTTGGCTT CACCAATTCC TGCTTTGGCA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.