NALCN gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_001149573.3	XP_001149573.1	sodium leak channel non-selective protein isoform X3
XM_001149573.4	XP_001149573.1	sodium leak channel non-selective protein isoform X3
XM_009427188.2	XP_009425463.1	sodium leak channel non-selective protein isoform X1
XM_009427189.2	XP_009425464.1	sodium leak channel non-selective protein isoform X1
XM_009427190.2	XP_009425465.1	sodium leak channel non-selective protein isoform X4
XM_024348325.1	XP_024204093.1	sodium leak channel non-selective protein isoform X3
XM_024348326.1	XP_024204094.1	sodium leak channel non-selective protein isoform X5
XM_009427189.3	XP_009425464.1	sodium leak channel non-selective protein isoform X1
XM_009427190.3	XP_009425465.1	sodium leak channel non-selective protein isoform X4
XM_016925503.1	XP_016780992.1	sodium leak channel non-selective protein isoform X2
XM_009427188.3	XP_009425463.1	sodium leak channel non-selective protein isoform X1
XM_016925503.2	XP_016780992.1	sodium leak channel non-selective protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc67543	XM_009427188.2 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc67543	XM_009427189.2 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc67544	XM_016925503.1 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc67545	XM_001149573.3 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc67546	XM_009427190.2 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc96894	XM_009427188.3 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96894	XM_009427189.3 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96895	XM_016925503.2 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96896	XM_001149573.4 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96896	XM_024348325.1 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96897	XM_009427190.3 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc96898	XM_024348326.1 Latest version!	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

NALCN ( XM_009427188.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425463.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc67543
Clone ID Related Accession (Same CDS sequence)	XM_009427188.2 , XM_009427189.2
Accession Version	XM_009427188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006480.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009427188.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGCGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATAGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425463.1
CDS	197..5500
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427188.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427188.2

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGCGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATAGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_009427189.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425464.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

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CloneID	OPc67543
Clone ID Related Accession (Same CDS sequence)	XM_009427188.2 , XM_009427189.2
Accession Version	XM_009427189.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006480.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009427189.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGCGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATAGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425464.1
CDS	94..5397
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427189.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427189.2

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGCGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATAGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_016925503.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780992.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc67544
Clone ID Related Accession (Same CDS sequence)	XM_016925503.1
Accession Version	XM_016925503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006480.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCTGAT TTGCTGGGAT TGGTCACTTA CCTGGACTGG 
GTCATGATCA TCGTAACCAT CTGCTCTTGC ATTTCCATGA TGTTTGAGTC CCCGTTTCGA 
AGAGTCATGC ATGCACCTAC TTTGCAGATT GCTGAGTATG TGTTTGTGAT ATTCATGAGC 
ATTGAGCTTA ATCTGAAGAT TATGGCAGAT GGCTTATTTT TCACTCCAAC TGCTGTCATC 
AGGGACTTCG GTGGAGTAAT GGACATATTT ATATATCTTG TGAGCTTGAT ATTTCTTTGT 
TGGATGCCTC AAAATGTACC TGCTGAATCG GGAGCTCAGC TTCTAATGGT CCTTCGGTGC 
CTGAGACCTC TGCGCATATT CAAACTGGTG CCCCAGATGA GGAAAGTTGT TCGAGAACTT 
TTCAGCGGCT TCAAGGAAAT TTTTTTGGTC TCCATTCTTT TGCTGACATT AATGCTCGTT 
TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT GGAAAACTGG CCAAGTGCAA TGATCCCAAC 
ATTATTAGAA GGGAAGATTG CAATGGCATA TTCAGAATTA ATGTCAGTGT GTCAAAGAAC 
TTAAATTTAA AATTGAGGCC TGGAGAGAAA AAACCTGGAT TTTGGGTGCC CCGTGTTTGG 
GCGAATCCTC GGAACTTTAA TTTCGACAAT GTGGGAAACG CTATGCTGGC GTTGTTTGAA 
GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG AGAGATGTTA TTATTCATCG TGTGGGGCCG 
ATCCATGGAA TCTATATTCA TGTTTTTGTA TTCCTGGGTT GCATGATTGG ACTGACCCTT 
TTTGTTGGAG TAGTTATTGC TAATTTCAAT GAAAACAAGG GGACGGCTTT GCTGACCGTC 
GATCAGAGAA GATGGGAAGA CCTGAAGAGC CGACTGAAGA TCGCACAGCC TCTTCATCTC 
CCGCCTCGCC CGGATAATGA TGGTTTTAGA GCTAAAATGT ATGACATAAC CCAGCATCCA 
TTTTTTAAGA GGACAATCGC ATTACTCGTC CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG 
TGGGACGTCG AGGACCCGGT GACCGTACCT TTGGCAACAA TGTCAGTTGT TTTCACCTTC 
ATCTTTGTTC TGGAGGTTAC CATGAAGATC ATAGCAATGT CGCCTGCTGG CTTCTGGCAA 
AGCAGAAGAA ACCGATATGA TCTCCTGGTG ACATCGCTTG GCGTTGTATG GGTGGTGCTT 
CACTTTGCCC TCCTGAATGC ATATACTTAC ATGATGGGCG CTTGTGTGAT TGTATTTAGG 
TTTTTCTCCA TCTGTGGAAA ACATGTAACG CTAAAGATGC TCCTCTTGAC AGTGGTCGTC 
AGCATGTACA AGAGCTTCTT TATCATAGTA GGCATGTTTC TCTTGCTGCT GTGTTACGCT 
TTTGCTGGAG TTGTTTTATT TGGTACTGTG AAATATGGGG AGAATATTAA CAGGCATGCA 
AATTTTTCTT CGGCTGGAAA AGCTATTACC GTACTGTTCC GAATTGTCAC AGGTGAAGAC 
TGGAACAAGA TTATGCATGA CTGTATGGTT CAGCCTCCGT TTTGTACTCC AGATGAATTT 
ACATACTGGG CAACAGACTG TGGAAATTAT GCTGGGGCAC TTATGTATTT CTGTTCATTT 
TATGTCATCA TTGCCTACAT CATGCTAAAT CTGCTTGTAG CCATAATTGT GGAGAATTTC 
TCCTTGTTTT ATTCCACTGA GGAGGACCAG CTTTTAAGTT ACAATGATCT TCGCCACTTT 
CAAATCATAT GGAACATGGT GGATGATAAA AGAGAGGGGG TGATCCCCAC GTTCCGCGTC 
AAGTTCCTGC TGCGGCTACT GCGTGGGAGG CTGGAGGTGG ACCTGGACAA GGACAAGCTC 
CTGTTTAAGC ACATGTGCTA CGAAATGGAG AGGCTCCACA ATGGCGGCGA CGTCACCTTC 
CATGATGTCC TGAGCATGCT TTCATACCGG TCCGTGGACA TCCGGAAGAG CTTGCAGCTG 
GAGGAACTCC TGGCGAGGGA GCAGCTGGAG TACACCATAG AGGAGGAGGT GGCCAAGCAG 
ACCATCCGCA TGTGGCTCAA GAAGTGCCTG AAGCGCATCA GAGCTAAACA GCAGCAGTCG 
TGCAGTATCA TCCACAGCCT GAGAGAGAGT CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC 
CCGCCCAGCA TCGAGACCAC CCAGCCCAGT GAGGACACGA ATGCCAACAG TCAGGACAAC 
AGCATGCAAC CTGAGACAAG CAGCCAGCAG CAGCTCCTGA GCCCCACGCT GTCGGATAGA 
GGAGGAAGTC GGCAAGATGC CGCCGATGCA GGGAAACCCC AGAGGAAATT TGGGCAGTGG 
CGTCTGCCCT CAGCCCCAAA ACCAATAAGC CATTCAGTGT CCTCGGTCAA CTTACGGTTT 
GGAGGAAGGA CAACCATGAA ATCTGTCGTG TGCAAAATGA ACCCCATGAC TGACGCGGCT 
TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG ACCCGGCAGC TGACTGTGGA GAGCGACGAA 
AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780992.1
CDS	197..5443
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNADLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_016925503.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016925503.1

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCTGAT TTGCTGGGAT TGGTCACTTA CCTGGACTGG 
GTCATGATCA TCGTAACCAT CTGCTCTTGC ATTTCCATGA TGTTTGAGTC CCCGTTTCGA 
AGAGTCATGC ATGCACCTAC TTTGCAGATT GCTGAGTATG TGTTTGTGAT ATTCATGAGC 
ATTGAGCTTA ATCTGAAGAT TATGGCAGAT GGCTTATTTT TCACTCCAAC TGCTGTCATC 
AGGGACTTCG GTGGAGTAAT GGACATATTT ATATATCTTG TGAGCTTGAT ATTTCTTTGT 
TGGATGCCTC AAAATGTACC TGCTGAATCG GGAGCTCAGC TTCTAATGGT CCTTCGGTGC 
CTGAGACCTC TGCGCATATT CAAACTGGTG CCCCAGATGA GGAAAGTTGT TCGAGAACTT 
TTCAGCGGCT TCAAGGAAAT TTTTTTGGTC TCCATTCTTT TGCTGACATT AATGCTCGTT 
TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT GGAAAACTGG CCAAGTGCAA TGATCCCAAC 
ATTATTAGAA GGGAAGATTG CAATGGCATA TTCAGAATTA ATGTCAGTGT GTCAAAGAAC 
TTAAATTTAA AATTGAGGCC TGGAGAGAAA AAACCTGGAT TTTGGGTGCC CCGTGTTTGG 
GCGAATCCTC GGAACTTTAA TTTCGACAAT GTGGGAAACG CTATGCTGGC GTTGTTTGAA 
GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG AGAGATGTTA TTATTCATCG TGTGGGGCCG 
ATCCATGGAA TCTATATTCA TGTTTTTGTA TTCCTGGGTT GCATGATTGG ACTGACCCTT 
TTTGTTGGAG TAGTTATTGC TAATTTCAAT GAAAACAAGG GGACGGCTTT GCTGACCGTC 
GATCAGAGAA GATGGGAAGA CCTGAAGAGC CGACTGAAGA TCGCACAGCC TCTTCATCTC 
CCGCCTCGCC CGGATAATGA TGGTTTTAGA GCTAAAATGT ATGACATAAC CCAGCATCCA 
TTTTTTAAGA GGACAATCGC ATTACTCGTC CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG 
TGGGACGTCG AGGACCCGGT GACCGTACCT TTGGCAACAA TGTCAGTTGT TTTCACCTTC 
ATCTTTGTTC TGGAGGTTAC CATGAAGATC ATAGCAATGT CGCCTGCTGG CTTCTGGCAA 
AGCAGAAGAA ACCGATATGA TCTCCTGGTG ACATCGCTTG GCGTTGTATG GGTGGTGCTT 
CACTTTGCCC TCCTGAATGC ATATACTTAC ATGATGGGCG CTTGTGTGAT TGTATTTAGG 
TTTTTCTCCA TCTGTGGAAA ACATGTAACG CTAAAGATGC TCCTCTTGAC AGTGGTCGTC 
AGCATGTACA AGAGCTTCTT TATCATAGTA GGCATGTTTC TCTTGCTGCT GTGTTACGCT 
TTTGCTGGAG TTGTTTTATT TGGTACTGTG AAATATGGGG AGAATATTAA CAGGCATGCA 
AATTTTTCTT CGGCTGGAAA AGCTATTACC GTACTGTTCC GAATTGTCAC AGGTGAAGAC 
TGGAACAAGA TTATGCATGA CTGTATGGTT CAGCCTCCGT TTTGTACTCC AGATGAATTT 
ACATACTGGG CAACAGACTG TGGAAATTAT GCTGGGGCAC TTATGTATTT CTGTTCATTT 
TATGTCATCA TTGCCTACAT CATGCTAAAT CTGCTTGTAG CCATAATTGT GGAGAATTTC 
TCCTTGTTTT ATTCCACTGA GGAGGACCAG CTTTTAAGTT ACAATGATCT TCGCCACTTT 
CAAATCATAT GGAACATGGT GGATGATAAA AGAGAGGGGG TGATCCCCAC GTTCCGCGTC 
AAGTTCCTGC TGCGGCTACT GCGTGGGAGG CTGGAGGTGG ACCTGGACAA GGACAAGCTC 
CTGTTTAAGC ACATGTGCTA CGAAATGGAG AGGCTCCACA ATGGCGGCGA CGTCACCTTC 
CATGATGTCC TGAGCATGCT TTCATACCGG TCCGTGGACA TCCGGAAGAG CTTGCAGCTG 
GAGGAACTCC TGGCGAGGGA GCAGCTGGAG TACACCATAG AGGAGGAGGT GGCCAAGCAG 
ACCATCCGCA TGTGGCTCAA GAAGTGCCTG AAGCGCATCA GAGCTAAACA GCAGCAGTCG 
TGCAGTATCA TCCACAGCCT GAGAGAGAGT CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC 
CCGCCCAGCA TCGAGACCAC CCAGCCCAGT GAGGACACGA ATGCCAACAG TCAGGACAAC 
AGCATGCAAC CTGAGACAAG CAGCCAGCAG CAGCTCCTGA GCCCCACGCT GTCGGATAGA 
GGAGGAAGTC GGCAAGATGC CGCCGATGCA GGGAAACCCC AGAGGAAATT TGGGCAGTGG 
CGTCTGCCCT CAGCCCCAAA ACCAATAAGC CATTCAGTGT CCTCGGTCAA CTTACGGTTT 
GGAGGAAGGA CAACCATGAA ATCTGTCGTG TGCAAAATGA ACCCCATGAC TGACGCGGCT 
TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG ACCCGGCAGC TGACTGTGGA GAGCGACGAA 
AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_001149573.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001149573.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.

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CloneID	OPc67545
Clone ID Related Accession (Same CDS sequence)	XM_001149573.3
Accession Version	XM_001149573.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006480.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001149573.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATAGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001149573.1
CDS	190..5406
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_001149573.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001149573.3

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGCGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATAGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_009427190.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425465.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.

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CloneID	OPc67546
Clone ID Related Accession (Same CDS sequence)	XM_009427190.2
Accession Version	XM_009427190.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006480.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009427190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAC TTCGCAAGTC TGTTTTCAGC 
ATCAGGGCAA GGAACCTTCT GGAAAAGGAG ACCGCAGTCA CTAAAATCTT AAGCAAGAGG 
CCATTACCGA CGAGCTCACC CATTAGCATC CGCTGTTGTC TCTACTTGGG AAGATCACAT 
TGTCACTCTA GTGGAGTCAG AGCTTGCACC CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCCG CAAAGGAGAG GTCAATCCTC AGCGTGCAGC ATCATATCCG CCAAGAGCGC 
AGGTCACTAA GACATGGATC GAACAGCCAG AGGATCAGCA GGGGAAAATC TCTTGAAACT 
TTGACTCAAG ATCATTCCAA TACAGTGAGA TATAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAGATGA TTCAGGAAAA AAAGGAGCAA GCAGAGATGA AAAGGAAAGT GCAAGAAGAG 
GAACTCAGAG AGAACCACCC ATACTTCGAT AAGCCACTGT TCATTGTCGG GCGAGAACAC 
AGGTTCAGAA ACTTTTGCCG GGTGGTGGTC CGAGCACGCT TCAACGCATC TAAAACAGAC 
CCTGTCACAG GAGCTGTGAA AAATACAAAG TACCATCAAC TTTATGATTT GCTGGGATTG 
GTCACTTACC TGGACTGGGT CATGATCATC GTAACCATCT GCTCTTGCAT TTCCATGATG 
TTTGAGTCCC CGTTTCGAAG AGTCATGCAT GCACCTACTT TGCAGATTGC TGAGTATGTG 
TTTGTGATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTATTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTCGGT GGAGTAATGG ACATATTTAT ATATCTTGTG 
AGCTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CTGAATCGGG AGCTCAGCTT 
CTAATGGTCC TTCGGTGCCT GAGACCTCTG CGCATATTCA AACTGGTGCC CCAGATGAGG 
AAAGTTGTTC GAGAACTTTT CAGCGGCTTC AAGGAAATTT TTTTGGTCTC CATTCTTTTG 
CTGACATTAA TGCTCGTTTT TGCAAGCTTT GGAGTTCAGC TTTTTGCTGG AAAACTGGCC 
AAGTGCAATG ATCCCAACAT TATTAGAAGG GAAGATTGCA ATGGCATATT CAGAATTAAT 
GTCAGTGTGT CAAAGAACTT AAATTTAAAA TTGAGGCCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC GAATCCTCGG AACTTTAATT TCGACAATGT GGGAAACGCT 
ATGCTGGCGT TGTTTGAAGT TCTCTCCTTG AAAGGCTGGG TGGAAGTGAG AGATGTTATT 
ATTCATCGTG TGGGGCCGAT CCATGGAATC TATATTCATG TTTTTGTATT CCTGGGTTGC 
ATGATTGGAC TGACCCTTTT TGTTGGAGTA GTTATTGCTA ATTTCAATGA AAACAAGGGG 
ACGGCTTTGC TGACCGTCGA TCAGAGAAGA TGGGAAGACC TGAAGAGCCG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC GCCTCGCCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATCGCAT TACTCGTCCT GGCCCAGTCG 
GTGTTGCTCT CTGTCAAGTG GGACGTCGAG GACCCGGTGA CCGTACCTTT GGCAACAATG 
TCAGTTGTTT TCACCTTCAT CTTTGTTCTG GAGGTTACCA TGAAGATCAT AGCAATGTCG 
CCTGCTGGCT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCCTGGTGAC ATCGCTTGGC 
GTTGTATGGG TGGTGCTTCA CTTTGCCCTC CTGAATGCAT ATACTTACAT GATGGGCGCT 
TGTGTGATTG TATTTAGGTT TTTCTCCATC TGTGGAAAAC ATGTAACGCT AAAGATGCTC 
CTCTTGACAG TGGTCGTCAG CATGTACAAG AGCTTCTTTA TCATAGTAGG CATGTTTCTC 
TTGCTGCTGT GTTACGCTTT TGCTGGAGTT GTTTTATTTG GTACTGTGAA ATATGGGGAG 
AATATTAACA GGCATGCAAA TTTTTCTTCG GCTGGAAAAG CTATTACCGT ACTGTTCCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGACT GTATGGTTCA GCCTCCGTTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GAAATTATGC TGGGGCACTT 
ATGTATTTCT GTTCATTTTA TGTCATCATT GCCTACATCA TGCTAAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC CTTGTTTTAT TCCACTGAGG AGGACCAGCT TTTAAGTTAC 
AATGATCTTC GCCACTTTCA AATCATATGG AACATGGTGG ATGATAAAAG AGAGGGGGTG 
ATCCCCACGT TCCGCGTCAA GTTCCTGCTG CGGCTACTGC GTGGGAGGCT GGAGGTGGAC 
CTGGACAAGG ACAAGCTCCT GTTTAAGCAC ATGTGCTACG AAATGGAGAG GCTCCACAAT 
GGCGGCGACG TCACCTTCCA TGATGTCCTG AGCATGCTTT CATACCGGTC CGTGGACATC 
CGGAAGAGCT TGCAGCTGGA GGAACTCCTG GCGAGGGAGC AGCTGGAGTA CACCATAGAG 
GAGGAGGTGG CCAAGCAGAC CATCCGCATG TGGCTCAAGA AGTGCCTGAA GCGCATCAGA 
GCTAAACAGC AGCAGTCGTG CAGTATCATC CACAGCCTGA GAGAGAGTCA GCAGCAAGAG 
CTGAGCCGGT TTCTGAACCC GCCCAGCATC GAGACCACCC AGCCCAGTGA GGACACGAAT 
GCCAACAGTC AGGACAACAG CATGCAACCT GAGACAAGCA GCCAGCAGCA GCTCCTGAGC 
CCCACGCTGT CGGATAGAGG AGGAAGTCGG CAAGATGCCG CCGATGCAGG GAAACCCCAG 
AGGAAATTTG GGCAGTGGCG TCTGCCCTCA GCCCCAAAAC CAATAAGCCA TTCAGTGTCC 
TCGGTCAACT TACGGTTTGG AGGAAGGACA ACCATGAAAT CTGTCGTGTG CAAAATGAAC 
CCCATGACTG ACGCGGCTTC CTGCGGTTCT GAAGTTAAGA AGTGGTGGAC CCGGCAGCTG 
ACTGTGGAGA GCGACGAAAG TGGGGATGAC CTTCTGGATA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425465.1
CDS	197..5221
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427190.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427190.2

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCAAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAC TTCGCAAGTC TGTTTTCAGC 
ATCAGGGCAA GGAACCTTCT GGAAAAGGAG ACCGCAGTCA CTAAAATCTT AAGCAAGAGG 
CCATTACCGA CGAGCTCACC CATTAGCATC CGCTGTTGTC TCTACTTGGG AAGATCACAT 
TGTCACTCTA GTGGAGTCAG AGCTTGCACC CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCCG CAAAGGAGAG GTCAATCCTC AGCGTGCAGC ATCATATCCG CCAAGAGCGC 
AGGTCACTAA GACATGGATC GAACAGCCAG AGGATCAGCA GGGGAAAATC TCTTGAAACT 
TTGACTCAAG ATCATTCCAA TACAGTGAGA TATAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAGATGA TTCAGGAAAA AAAGGAGCAA GCAGAGATGA AAAGGAAAGT GCAAGAAGAG 
GAACTCAGAG AGAACCACCC ATACTTCGAT AAGCCACTGT TCATTGTCGG GCGAGAACAC 
AGGTTCAGAA ACTTTTGCCG GGTGGTGGTC CGAGCACGCT TCAACGCATC TAAAACAGAC 
CCTGTCACAG GAGCTGTGAA AAATACAAAG TACCATCAAC TTTATGATTT GCTGGGATTG 
GTCACTTACC TGGACTGGGT CATGATCATC GTAACCATCT GCTCTTGCAT TTCCATGATG 
TTTGAGTCCC CGTTTCGAAG AGTCATGCAT GCACCTACTT TGCAGATTGC TGAGTATGTG 
TTTGTGATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTATTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTCGGT GGAGTAATGG ACATATTTAT ATATCTTGTG 
AGCTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CTGAATCGGG AGCTCAGCTT 
CTAATGGTCC TTCGGTGCCT GAGACCTCTG CGCATATTCA AACTGGTGCC CCAGATGAGG 
AAAGTTGTTC GAGAACTTTT CAGCGGCTTC AAGGAAATTT TTTTGGTCTC CATTCTTTTG 
CTGACATTAA TGCTCGTTTT TGCAAGCTTT GGAGTTCAGC TTTTTGCTGG AAAACTGGCC 
AAGTGCAATG ATCCCAACAT TATTAGAAGG GAAGATTGCA ATGGCATATT CAGAATTAAT 
GTCAGTGTGT CAAAGAACTT AAATTTAAAA TTGAGGCCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC GAATCCTCGG AACTTTAATT TCGACAATGT GGGAAACGCT 
ATGCTGGCGT TGTTTGAAGT TCTCTCCTTG AAAGGCTGGG TGGAAGTGAG AGATGTTATT 
ATTCATCGTG TGGGGCCGAT CCATGGAATC TATATTCATG TTTTTGTATT CCTGGGTTGC 
ATGATTGGAC TGACCCTTTT TGTTGGAGTA GTTATTGCTA ATTTCAATGA AAACAAGGGG 
ACGGCTTTGC TGACCGTCGA TCAGAGAAGA TGGGAAGACC TGAAGAGCCG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC GCCTCGCCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATCGCAT TACTCGTCCT GGCCCAGTCG 
GTGTTGCTCT CTGTCAAGTG GGACGTCGAG GACCCGGTGA CCGTACCTTT GGCAACAATG 
TCAGTTGTTT TCACCTTCAT CTTTGTTCTG GAGGTTACCA TGAAGATCAT AGCAATGTCG 
CCTGCTGGCT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCCTGGTGAC ATCGCTTGGC 
GTTGTATGGG TGGTGCTTCA CTTTGCCCTC CTGAATGCAT ATACTTACAT GATGGGCGCT 
TGTGTGATTG TATTTAGGTT TTTCTCCATC TGTGGAAAAC ATGTAACGCT AAAGATGCTC 
CTCTTGACAG TGGTCGTCAG CATGTACAAG AGCTTCTTTA TCATAGTAGG CATGTTTCTC 
TTGCTGCTGT GTTACGCTTT TGCTGGAGTT GTTTTATTTG GTACTGTGAA ATATGGGGAG 
AATATTAACA GGCATGCAAA TTTTTCTTCG GCTGGAAAAG CTATTACCGT ACTGTTCCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGACT GTATGGTTCA GCCTCCGTTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GAAATTATGC TGGGGCACTT 
ATGTATTTCT GTTCATTTTA TGTCATCATT GCCTACATCA TGCTAAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC CTTGTTTTAT TCCACTGAGG AGGACCAGCT TTTAAGTTAC 
AATGATCTTC GCCACTTTCA AATCATATGG AACATGGTGG ATGATAAAAG AGAGGGGGTG 
ATCCCCACGT TCCGCGTCAA GTTCCTGCTG CGGCTACTGC GTGGGAGGCT GGAGGTGGAC 
CTGGACAAGG ACAAGCTCCT GTTTAAGCAC ATGTGCTACG AAATGGAGAG GCTCCACAAT 
GGCGGCGACG TCACCTTCCA TGATGTCCTG AGCATGCTTT CATACCGGTC CGTGGACATC 
CGGAAGAGCT TGCAGCTGGA GGAACTCCTG GCGAGGGAGC AGCTGGAGTA CACCATAGAG 
GAGGAGGTGG CCAAGCAGAC CATCCGCATG TGGCTCAAGA AGTGCCTGAA GCGCATCAGA 
GCTAAACAGC AGCAGTCGTG CAGTATCATC CACAGCCTGA GAGAGAGTCA GCAGCAAGAG 
CTGAGCCGGT TTCTGAACCC GCCCAGCATC GAGACCACCC AGCCCAGTGA GGACACGAAT 
GCCAACAGTC AGGACAACAG CATGCAACCT GAGACAAGCA GCCAGCAGCA GCTCCTGAGC 
CCCACGCTGT CGGATAGAGG AGGAAGTCGG CAAGATGCCG CCGATGCAGG GAAACCCCAG 
AGGAAATTTG GGCAGTGGCG TCTGCCCTCA GCCCCAAAAC CAATAAGCCA TTCAGTGTCC 
TCGGTCAACT TACGGTTTGG AGGAAGGACA ACCATGAAAT CTGTCGTGTG CAAAATGAAC 
CCCATGACTG ACGCGGCTTC CTGCGGTTCT GAAGTTAAGA AGTGGTGGAC CCGGCAGCTG 
ACTGTGGAGA GCGACGAAAG TGGGGATGAC CTTCTGGATA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_009427189.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425464.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

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CloneID	OPc96894
Clone ID Related Accession (Same CDS sequence)	XM_009427189.3 , XM_009427188.3
Accession Version	XM_009427189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009427189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGTGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATCGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425464.1
CDS	94..5397
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427189.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427189.3

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGTGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATCGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_009427188.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425463.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

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CloneID	OPc96894
Clone ID Related Accession (Same CDS sequence)	XM_009427189.3 , XM_009427188.3
Accession Version	XM_009427188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009427188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGTGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATCGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425463.1
CDS	231..5534
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427188.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427188.3

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCATCT AAAACAGACC CTGTCACAGG AGCTGTGAAA 
AATACAAAGT ACCATCAACT TTATGATTTG CTGGGATTGG TCACTTACCT GGACTGGGTC 
ATGATCATCG TAACCATCTG CTCTTGCATT TCCATGATGT TTGAGTCCCC GTTTCGAAGA 
GTCATGCATG CACCTACTTT GCAGATTGCT GAGTATGTGT TTGTGATATT CATGAGCATT 
GAGCTTAATC TGAAGATTAT GGCAGATGGC TTATTTTTCA CTCCAACTGC TGTCATCAGG 
GACTTCGGTG GAGTAATGGA CATATTTATA TATCTTGTGA GCTTGATATT TCTTTGTTGG 
ATGCCTCAAA ATGTACCTGC TGAATCGGGA GCTCAGCTTC TAATGGTCCT TCGGTGCCTG 
AGACCTCTGC GCATATTCAA ACTGGTGCCC CAGATGAGGA AAGTTGTTCG AGAACTTTTC 
AGTGGCTTCA AGGAAATTTT TTTGGTCTCC ATTCTTTTGC TGACATTAAT GCTCGTTTTT 
GCAAGCTTTG GAGTTCAGCT TTTTGCTGGA AAACTGGCCA AGTGCAATGA TCCCAACATT 
ATTAGAAGGG AAGATTGCAA TGGCATATTC AGAATTAATG TCAGTGTGTC AAAGAACTTA 
AATTTAAAAT TGAGGCCTGG AGAGAAAAAA CCTGGATTTT GGGTGCCCCG TGTTTGGGCG 
AATCCTCGGA ACTTTAATTT CGACAATGTG GGAAACGCTA TGCTGGCGTT GTTTGAAGTT 
CTCTCCTTGA AAGGCTGGGT GGAAGTGAGA GATGTTATTA TTCATCGTGT GGGGCCGATC 
CATGGAATCT ATATTCATGT TTTTGTATTC CTGGGTTGCA TGATTGGACT GACCCTTTTT 
GTTGGAGTAG TTATTGCTAA TTTCAATGAA AACAAGGGGA CGGCTTTGCT GACCGTCGAT 
CAGAGAAGAT GGGAAGACCT GAAGAGCCGA CTGAAGATCG CACAGCCTCT TCATCTCCCG 
CCTCGCCCGG ATAATGATGG TTTTAGAGCT AAAATGTATG ACATAACCCA GCATCCATTT 
TTTAAGAGGA CAATCGCATT ACTCGTCCTG GCCCAGTCGG TGTTGCTCTC TGTCAAGTGG 
GACGTCGAGG ACCCGGTGAC CGTACCTTTG GCAACAATGT CAGTTGTTTT CACCTTCATC 
TTTGTTCTGG AGGTTACCAT GAAGATCATA GCAATGTCGC CTGCTGGCTT CTGGCAAAGC 
AGAAGAAACC GATATGATCT CCTGGTGACA TCGCTTGGCG TTGTATGGGT GGTGCTTCAC 
TTTGCCCTCC TGAATGCATA TACTTACATG ATGGGCGCTT GTGTGATTGT ATTTAGGTTT 
TTCTCCATCT GTGGAAAACA TGTAACGCTA AAGATGCTCC TCTTGACAGT GGTCGTCAGC 
ATGTACAAGA GCTTCTTTAT CATAGTAGGC ATGTTTCTCT TGCTGCTGTG TTACGCTTTT 
GCTGGAGTTG TTTTATTTGG TACTGTGAAA TATGGGGAGA ATATTAACAG GCATGCAAAT 
TTTTCTTCGG CTGGAAAAGC TATTACCGTA CTGTTCCGAA TTGTCACAGG TGAAGACTGG 
AACAAGATTA TGCATGACTG TATGGTTCAG CCTCCGTTTT GTACTCCAGA TGAATTTACA 
TACTGGGCAA CAGACTGTGG AAATTATGCT GGGGCACTTA TGTATTTCTG TTCATTTTAT 
GTCATCATTG CCTACATCAT GCTAAATCTG CTTGTAGCCA TAATTGTGGA GAATTTCTCC 
TTGTTTTATT CCACTGAGGA GGACCAGCTT TTAAGTTACA ATGATCTTCG CCACTTTCAA 
ATCATATGGA ACATGGTGGA TGATAAAAGA GAGGGGGTGA TCCCCACGTT CCGCGTCAAG 
TTCCTGCTGC GGCTACTGCG TGGGAGGCTG GAGGTGGACC TGGACAAGGA CAAGCTCCTG 
TTTAAGCACA TGTGCTACGA AATGGAGAGG CTCCACAATG GCGGCGACGT CACCTTCCAT 
GATGTCCTGA GCATGCTTTC ATACCGGTCC GTGGACATCC GGAAGAGCTT GCAGCTGGAG 
GAACTCCTGG CGAGGGAGCA GCTGGAGTAC ACCATAGAGG AGGAGGTGGC CAAGCAGACC 
ATCCGCATGT GGCTCAAGAA GTGCCTGAAG CGCATCAGAG CTAAACAGCA GCAGTCGTGC 
AGTATCATCC ACAGCCTGAG AGAGAGTCAG CAGCAAGAGC TGAGCCGGTT TCTGAACCCG 
CCCAGCATCG AGACCACCCA GCCCAGTGAG GACACGAATG CCAACAGTCA GGACAACAGC 
ATGCAACCTG AGACAAGCAG CCAGCAGCAG CTCCTGAGCC CCACGCTGTC GGATCGAGGA 
GGAAGTCGGC AAGATGCCGC CGATGCAGGG AAACCCCAGA GGAAATTTGG GCAGTGGCGT 
CTGCCCTCAG CCCCAAAACC AATAAGCCAT TCAGTGTCCT CGGTCAACTT ACGGTTTGGA 
GGAAGGACAA CCATGAAATC TGTCGTGTGC AAAATGAACC CCATGACTGA CGCGGCTTCC 
TGCGGTTCTG AAGTTAAGAA GTGGTGGACC CGGCAGCTGA CTGTGGAGAG CGACGAAAGT 
GGGGATGACC TTCTGGATAT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_016925503.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780992.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

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CloneID	OPc96895
Clone ID Related Accession (Same CDS sequence)	XM_016925503.2
Accession Version	XM_016925503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016925503.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCTGAT TTGCTGGGAT TGGTCACTTA CCTGGACTGG 
GTCATGATCA TCGTAACCAT CTGCTCTTGC ATTTCCATGA TGTTTGAGTC CCCGTTTCGA 
AGAGTCATGC ATGCACCTAC TTTGCAGATT GCTGAGTATG TGTTTGTGAT ATTCATGAGC 
ATTGAGCTTA ATCTGAAGAT TATGGCAGAT GGCTTATTTT TCACTCCAAC TGCTGTCATC 
AGGGACTTCG GTGGAGTAAT GGACATATTT ATATATCTTG TGAGCTTGAT ATTTCTTTGT 
TGGATGCCTC AAAATGTACC TGCTGAATCG GGAGCTCAGC TTCTAATGGT CCTTCGGTGC 
CTGAGACCTC TGCGCATATT CAAACTGGTG CCCCAGATGA GGAAAGTTGT TCGAGAACTT 
TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC TCCATTCTTT TGCTGACATT AATGCTCGTT 
TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT GGAAAACTGG CCAAGTGCAA TGATCCCAAC 
ATTATTAGAA GGGAAGATTG CAATGGCATA TTCAGAATTA ATGTCAGTGT GTCAAAGAAC 
TTAAATTTAA AATTGAGGCC TGGAGAGAAA AAACCTGGAT TTTGGGTGCC CCGTGTTTGG 
GCGAATCCTC GGAACTTTAA TTTCGACAAT GTGGGAAACG CTATGCTGGC GTTGTTTGAA 
GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG AGAGATGTTA TTATTCATCG TGTGGGGCCG 
ATCCATGGAA TCTATATTCA TGTTTTTGTA TTCCTGGGTT GCATGATTGG ACTGACCCTT 
TTTGTTGGAG TAGTTATTGC TAATTTCAAT GAAAACAAGG GGACGGCTTT GCTGACCGTC 
GATCAGAGAA GATGGGAAGA CCTGAAGAGC CGACTGAAGA TCGCACAGCC TCTTCATCTC 
CCGCCTCGCC CGGATAATGA TGGTTTTAGA GCTAAAATGT ATGACATAAC CCAGCATCCA 
TTTTTTAAGA GGACAATCGC ATTACTCGTC CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG 
TGGGACGTCG AGGACCCGGT GACCGTACCT TTGGCAACAA TGTCAGTTGT TTTCACCTTC 
ATCTTTGTTC TGGAGGTTAC CATGAAGATC ATAGCAATGT CGCCTGCTGG CTTCTGGCAA 
AGCAGAAGAA ACCGATATGA TCTCCTGGTG ACATCGCTTG GCGTTGTATG GGTGGTGCTT 
CACTTTGCCC TCCTGAATGC ATATACTTAC ATGATGGGCG CTTGTGTGAT TGTATTTAGG 
TTTTTCTCCA TCTGTGGAAA ACATGTAACG CTAAAGATGC TCCTCTTGAC AGTGGTCGTC 
AGCATGTACA AGAGCTTCTT TATCATAGTA GGCATGTTTC TCTTGCTGCT GTGTTACGCT 
TTTGCTGGAG TTGTTTTATT TGGTACTGTG AAATATGGGG AGAATATTAA CAGGCATGCA 
AATTTTTCTT CGGCTGGAAA AGCTATTACC GTACTGTTCC GAATTGTCAC AGGTGAAGAC 
TGGAACAAGA TTATGCATGA CTGTATGGTT CAGCCTCCGT TTTGTACTCC AGATGAATTT 
ACATACTGGG CAACAGACTG TGGAAATTAT GCTGGGGCAC TTATGTATTT CTGTTCATTT 
TATGTCATCA TTGCCTACAT CATGCTAAAT CTGCTTGTAG CCATAATTGT GGAGAATTTC 
TCCTTGTTTT ATTCCACTGA GGAGGACCAG CTTTTAAGTT ACAATGATCT TCGCCACTTT 
CAAATCATAT GGAACATGGT GGATGATAAA AGAGAGGGGG TGATCCCCAC GTTCCGCGTC 
AAGTTCCTGC TGCGGCTACT GCGTGGGAGG CTGGAGGTGG ACCTGGACAA GGACAAGCTC 
CTGTTTAAGC ACATGTGCTA CGAAATGGAG AGGCTCCACA ATGGCGGCGA CGTCACCTTC 
CATGATGTCC TGAGCATGCT TTCATACCGG TCCGTGGACA TCCGGAAGAG CTTGCAGCTG 
GAGGAACTCC TGGCGAGGGA GCAGCTGGAG TACACCATAG AGGAGGAGGT GGCCAAGCAG 
ACCATCCGCA TGTGGCTCAA GAAGTGCCTG AAGCGCATCA GAGCTAAACA GCAGCAGTCG 
TGCAGTATCA TCCACAGCCT GAGAGAGAGT CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC 
CCGCCCAGCA TCGAGACCAC CCAGCCCAGT GAGGACACGA ATGCCAACAG TCAGGACAAC 
AGCATGCAAC CTGAGACAAG CAGCCAGCAG CAGCTCCTGA GCCCCACGCT GTCGGATCGA 
GGAGGAAGTC GGCAAGATGC CGCCGATGCA GGGAAACCCC AGAGGAAATT TGGGCAGTGG 
CGTCTGCCCT CAGCCCCAAA ACCAATAAGC CATTCAGTGT CCTCGGTCAA CTTACGGTTT 
GGAGGAAGGA CAACCATGAA ATCTGTCGTG TGCAAAATGA ACCCCATGAC TGACGCGGCT 
TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG ACCCGGCAGC TGACTGTGGA GAGCGACGAA 
AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780992.1
CDS	197..5443
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNADLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_016925503.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016925503.2

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGCAAGAGGC CATTACCGAC GAGCTCACCC 
ATTAGCATCC GCTGTTGTCT CTACTTGGGA AGATCACATT GTCACTCTAG TGGAGTCAGA 
GCTTGCACCC GACAGCGCAT GCTGAGCGGA TCATTCGAGG GGCAGCCCGC AAAGGAGAGG 
TCAATCCTCA GCGTGCAGCA TCATATCCGC CAAGAGCGCA GGTCACTAAG ACATGGATCG 
AACAGCCAGA GGATCAGCAG GGGAAAATCT CTTGAAACTT TGACTCAAGA TCATTCCAAT 
ACAGTGAGAT ATAGAAATGC ACAAAGAGAA GACAGTGAAA TAAAGATGAT TCAGGAAAAA 
AAGGAGCAAG CAGAGATGAA AAGGAAAGTG CAAGAAGAGG AACTCAGAGA GAACCACCCA 
TACTTCGATA AGCCACTGTT CATTGTCGGG CGAGAACACA GGTTCAGAAA CTTTTGCCGG 
GTGGTGGTCC GAGCACGCTT CAACGCTGAT TTGCTGGGAT TGGTCACTTA CCTGGACTGG 
GTCATGATCA TCGTAACCAT CTGCTCTTGC ATTTCCATGA TGTTTGAGTC CCCGTTTCGA 
AGAGTCATGC ATGCACCTAC TTTGCAGATT GCTGAGTATG TGTTTGTGAT ATTCATGAGC 
ATTGAGCTTA ATCTGAAGAT TATGGCAGAT GGCTTATTTT TCACTCCAAC TGCTGTCATC 
AGGGACTTCG GTGGAGTAAT GGACATATTT ATATATCTTG TGAGCTTGAT ATTTCTTTGT 
TGGATGCCTC AAAATGTACC TGCTGAATCG GGAGCTCAGC TTCTAATGGT CCTTCGGTGC 
CTGAGACCTC TGCGCATATT CAAACTGGTG CCCCAGATGA GGAAAGTTGT TCGAGAACTT 
TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC TCCATTCTTT TGCTGACATT AATGCTCGTT 
TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT GGAAAACTGG CCAAGTGCAA TGATCCCAAC 
ATTATTAGAA GGGAAGATTG CAATGGCATA TTCAGAATTA ATGTCAGTGT GTCAAAGAAC 
TTAAATTTAA AATTGAGGCC TGGAGAGAAA AAACCTGGAT TTTGGGTGCC CCGTGTTTGG 
GCGAATCCTC GGAACTTTAA TTTCGACAAT GTGGGAAACG CTATGCTGGC GTTGTTTGAA 
GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG AGAGATGTTA TTATTCATCG TGTGGGGCCG 
ATCCATGGAA TCTATATTCA TGTTTTTGTA TTCCTGGGTT GCATGATTGG ACTGACCCTT 
TTTGTTGGAG TAGTTATTGC TAATTTCAAT GAAAACAAGG GGACGGCTTT GCTGACCGTC 
GATCAGAGAA GATGGGAAGA CCTGAAGAGC CGACTGAAGA TCGCACAGCC TCTTCATCTC 
CCGCCTCGCC CGGATAATGA TGGTTTTAGA GCTAAAATGT ATGACATAAC CCAGCATCCA 
TTTTTTAAGA GGACAATCGC ATTACTCGTC CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG 
TGGGACGTCG AGGACCCGGT GACCGTACCT TTGGCAACAA TGTCAGTTGT TTTCACCTTC 
ATCTTTGTTC TGGAGGTTAC CATGAAGATC ATAGCAATGT CGCCTGCTGG CTTCTGGCAA 
AGCAGAAGAA ACCGATATGA TCTCCTGGTG ACATCGCTTG GCGTTGTATG GGTGGTGCTT 
CACTTTGCCC TCCTGAATGC ATATACTTAC ATGATGGGCG CTTGTGTGAT TGTATTTAGG 
TTTTTCTCCA TCTGTGGAAA ACATGTAACG CTAAAGATGC TCCTCTTGAC AGTGGTCGTC 
AGCATGTACA AGAGCTTCTT TATCATAGTA GGCATGTTTC TCTTGCTGCT GTGTTACGCT 
TTTGCTGGAG TTGTTTTATT TGGTACTGTG AAATATGGGG AGAATATTAA CAGGCATGCA 
AATTTTTCTT CGGCTGGAAA AGCTATTACC GTACTGTTCC GAATTGTCAC AGGTGAAGAC 
TGGAACAAGA TTATGCATGA CTGTATGGTT CAGCCTCCGT TTTGTACTCC AGATGAATTT 
ACATACTGGG CAACAGACTG TGGAAATTAT GCTGGGGCAC TTATGTATTT CTGTTCATTT 
TATGTCATCA TTGCCTACAT CATGCTAAAT CTGCTTGTAG CCATAATTGT GGAGAATTTC 
TCCTTGTTTT ATTCCACTGA GGAGGACCAG CTTTTAAGTT ACAATGATCT TCGCCACTTT 
CAAATCATAT GGAACATGGT GGATGATAAA AGAGAGGGGG TGATCCCCAC GTTCCGCGTC 
AAGTTCCTGC TGCGGCTACT GCGTGGGAGG CTGGAGGTGG ACCTGGACAA GGACAAGCTC 
CTGTTTAAGC ACATGTGCTA CGAAATGGAG AGGCTCCACA ATGGCGGCGA CGTCACCTTC 
CATGATGTCC TGAGCATGCT TTCATACCGG TCCGTGGACA TCCGGAAGAG CTTGCAGCTG 
GAGGAACTCC TGGCGAGGGA GCAGCTGGAG TACACCATAG AGGAGGAGGT GGCCAAGCAG 
ACCATCCGCA TGTGGCTCAA GAAGTGCCTG AAGCGCATCA GAGCTAAACA GCAGCAGTCG 
TGCAGTATCA TCCACAGCCT GAGAGAGAGT CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC 
CCGCCCAGCA TCGAGACCAC CCAGCCCAGT GAGGACACGA ATGCCAACAG TCAGGACAAC 
AGCATGCAAC CTGAGACAAG CAGCCAGCAG CAGCTCCTGA GCCCCACGCT GTCGGATCGA 
GGAGGAAGTC GGCAAGATGC CGCCGATGCA GGGAAACCCC AGAGGAAATT TGGGCAGTGG 
CGTCTGCCCT CAGCCCCAAA ACCAATAAGC CATTCAGTGT CCTCGGTCAA CTTACGGTTT 
GGAGGAAGGA CAACCATGAA ATCTGTCGTG TGCAAAATGA ACCCCATGAC TGACGCGGCT 
TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG ACCCGGCAGC TGACTGTGGA GAGCGACGAA 
AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_001149573.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001149573.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.

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CloneID	OPc96896
Clone ID Related Accession (Same CDS sequence)	XM_001149573.4 , XM_024348325.1
Accession Version	XM_001149573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_001149573.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATCGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001149573.1
CDS	236..5452
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_001149573.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001149573.4

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATCGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_024348325.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204093.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.

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CloneID	OPc96896
Clone ID Related Accession (Same CDS sequence)	XM_001149573.4 , XM_024348325.1
Accession Version	XM_024348325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATCGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204093.1
CDS	113..5329
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_024348325.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348325.1

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAT TAAAGCAAAG TGAAGCAAAT 
GCGGACACCA AAGAAAAGCT CCCTTTACGC CTGCGAATCT TTGAAAAATT TCCAAACAGA 
CCTCAAATGG TGAAAATCTC AAAGCTTCCT TCAGATTTTA CAGTTCCTAA AATCAGGGAG 
AGTTTTATGA AGCAGTTTAT TGACCGCCAG CAACAGGACA CATGTTGCCT CCTGAGAAGC 
CTCCCGACCA CCTCTTCCTC CTCCTGCGAC CACTCCAAAC GCTCGGCAAT TGAGGACAAC 
AAATACATCG ACCAAAAACT TCGCAAGTCT GTTTTCAGCA TCAGGGCAAG GAACCTTCTG 
GAAAAGGAGA CCGCAGTCAC TAAAATCTTA AGAGCTTGCA CCCGACAGCG CATGCTGAGC 
GGATCATTCG AGGGGCAGCC CGCAAAGGAG AGGTCAATCC TCAGCGTGCA GCATCATATC 
CGCCAAGAGC GCAGGTCACT AAGACATGGA TCGAACAGCC AGAGGATCAG CAGGGGAAAA 
TCTCTTGAAA CTTTGACTCA AGATCATTCC AATACAGTGA GATATAGAAA TGCACAAAGA 
GAAGACAGTG AAATAAAGAT GATTCAGGAA AAAAAGGAGC AAGCAGAGAT GAAAAGGAAA 
GTGCAAGAAG AGGAACTCAG AGAGAACCAC CCATACTTCG ATAAGCCACT GTTCATTGTC 
GGGCGAGAAC ACAGGTTCAG AAACTTTTGC CGGGTGGTGG TCCGAGCACG CTTCAACGCA 
TCTAAAACAG ACCCTGTCAC AGGAGCTGTG AAAAATACAA AGTACCATCA ACTTTATGAT 
TTGCTGGGAT TGGTCACTTA CCTGGACTGG GTCATGATCA TCGTAACCAT CTGCTCTTGC 
ATTTCCATGA TGTTTGAGTC CCCGTTTCGA AGAGTCATGC ATGCACCTAC TTTGCAGATT 
GCTGAGTATG TGTTTGTGAT ATTCATGAGC ATTGAGCTTA ATCTGAAGAT TATGGCAGAT 
GGCTTATTTT TCACTCCAAC TGCTGTCATC AGGGACTTCG GTGGAGTAAT GGACATATTT 
ATATATCTTG TGAGCTTGAT ATTTCTTTGT TGGATGCCTC AAAATGTACC TGCTGAATCG 
GGAGCTCAGC TTCTAATGGT CCTTCGGTGC CTGAGACCTC TGCGCATATT CAAACTGGTG 
CCCCAGATGA GGAAAGTTGT TCGAGAACTT TTCAGTGGCT TCAAGGAAAT TTTTTTGGTC 
TCCATTCTTT TGCTGACATT AATGCTCGTT TTTGCAAGCT TTGGAGTTCA GCTTTTTGCT 
GGAAAACTGG CCAAGTGCAA TGATCCCAAC ATTATTAGAA GGGAAGATTG CAATGGCATA 
TTCAGAATTA ATGTCAGTGT GTCAAAGAAC TTAAATTTAA AATTGAGGCC TGGAGAGAAA 
AAACCTGGAT TTTGGGTGCC CCGTGTTTGG GCGAATCCTC GGAACTTTAA TTTCGACAAT 
GTGGGAAACG CTATGCTGGC GTTGTTTGAA GTTCTCTCCT TGAAAGGCTG GGTGGAAGTG 
AGAGATGTTA TTATTCATCG TGTGGGGCCG ATCCATGGAA TCTATATTCA TGTTTTTGTA 
TTCCTGGGTT GCATGATTGG ACTGACCCTT TTTGTTGGAG TAGTTATTGC TAATTTCAAT 
GAAAACAAGG GGACGGCTTT GCTGACCGTC GATCAGAGAA GATGGGAAGA CCTGAAGAGC 
CGACTGAAGA TCGCACAGCC TCTTCATCTC CCGCCTCGCC CGGATAATGA TGGTTTTAGA 
GCTAAAATGT ATGACATAAC CCAGCATCCA TTTTTTAAGA GGACAATCGC ATTACTCGTC 
CTGGCCCAGT CGGTGTTGCT CTCTGTCAAG TGGGACGTCG AGGACCCGGT GACCGTACCT 
TTGGCAACAA TGTCAGTTGT TTTCACCTTC ATCTTTGTTC TGGAGGTTAC CATGAAGATC 
ATAGCAATGT CGCCTGCTGG CTTCTGGCAA AGCAGAAGAA ACCGATATGA TCTCCTGGTG 
ACATCGCTTG GCGTTGTATG GGTGGTGCTT CACTTTGCCC TCCTGAATGC ATATACTTAC 
ATGATGGGCG CTTGTGTGAT TGTATTTAGG TTTTTCTCCA TCTGTGGAAA ACATGTAACG 
CTAAAGATGC TCCTCTTGAC AGTGGTCGTC AGCATGTACA AGAGCTTCTT TATCATAGTA 
GGCATGTTTC TCTTGCTGCT GTGTTACGCT TTTGCTGGAG TTGTTTTATT TGGTACTGTG 
AAATATGGGG AGAATATTAA CAGGCATGCA AATTTTTCTT CGGCTGGAAA AGCTATTACC 
GTACTGTTCC GAATTGTCAC AGGTGAAGAC TGGAACAAGA TTATGCATGA CTGTATGGTT 
CAGCCTCCGT TTTGTACTCC AGATGAATTT ACATACTGGG CAACAGACTG TGGAAATTAT 
GCTGGGGCAC TTATGTATTT CTGTTCATTT TATGTCATCA TTGCCTACAT CATGCTAAAT 
CTGCTTGTAG CCATAATTGT GGAGAATTTC TCCTTGTTTT ATTCCACTGA GGAGGACCAG 
CTTTTAAGTT ACAATGATCT TCGCCACTTT CAAATCATAT GGAACATGGT GGATGATAAA 
AGAGAGGGGG TGATCCCCAC GTTCCGCGTC AAGTTCCTGC TGCGGCTACT GCGTGGGAGG 
CTGGAGGTGG ACCTGGACAA GGACAAGCTC CTGTTTAAGC ACATGTGCTA CGAAATGGAG 
AGGCTCCACA ATGGCGGCGA CGTCACCTTC CATGATGTCC TGAGCATGCT TTCATACCGG 
TCCGTGGACA TCCGGAAGAG CTTGCAGCTG GAGGAACTCC TGGCGAGGGA GCAGCTGGAG 
TACACCATAG AGGAGGAGGT GGCCAAGCAG ACCATCCGCA TGTGGCTCAA GAAGTGCCTG 
AAGCGCATCA GAGCTAAACA GCAGCAGTCG TGCAGTATCA TCCACAGCCT GAGAGAGAGT 
CAGCAGCAAG AGCTGAGCCG GTTTCTGAAC CCGCCCAGCA TCGAGACCAC CCAGCCCAGT 
GAGGACACGA ATGCCAACAG TCAGGACAAC AGCATGCAAC CTGAGACAAG CAGCCAGCAG 
CAGCTCCTGA GCCCCACGCT GTCGGATCGA GGAGGAAGTC GGCAAGATGC CGCCGATGCA 
GGGAAACCCC AGAGGAAATT TGGGCAGTGG CGTCTGCCCT CAGCCCCAAA ACCAATAAGC 
CATTCAGTGT CCTCGGTCAA CTTACGGTTT GGAGGAAGGA CAACCATGAA ATCTGTCGTG 
TGCAAAATGA ACCCCATGAC TGACGCGGCT TCCTGCGGTT CTGAAGTTAA GAAGTGGTGG 
ACCCGGCAGC TGACTGTGGA GAGCGACGAA AGTGGGGATG ACCTTCTGGA TATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_009427190.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009425465.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X6, mRNA.

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CloneID	OPc96897
Clone ID Related Accession (Same CDS sequence)	XM_009427190.3
Accession Version	XM_009427190.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009427190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAC TTCGCAAGTC TGTTTTCAGC 
ATCAGGGCAA GGAACCTTCT GGAAAAGGAG ACCGCAGTCA CTAAAATCTT AAGCAAGAGG 
CCATTACCGA CGAGCTCACC CATTAGCATC CGCTGTTGTC TCTACTTGGG AAGATCACAT 
TGTCACTCTA GTGGAGTCAG AGCTTGCACC CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCCG CAAAGGAGAG GTCAATCCTC AGCGTGCAGC ATCATATCCG CCAAGAGCGC 
AGGTCACTAA GACATGGATC GAACAGCCAG AGGATCAGCA GGGGAAAATC TCTTGAAACT 
TTGACTCAAG ATCATTCCAA TACAGTGAGA TATAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAGATGA TTCAGGAAAA AAAGGAGCAA GCAGAGATGA AAAGGAAAGT GCAAGAAGAG 
GAACTCAGAG AGAACCACCC ATACTTCGAT AAGCCACTGT TCATTGTCGG GCGAGAACAC 
AGGTTCAGAA ACTTTTGCCG GGTGGTGGTC CGAGCACGCT TCAACGCATC TAAAACAGAC 
CCTGTCACAG GAGCTGTGAA AAATACAAAG TACCATCAAC TTTATGATTT GCTGGGATTG 
GTCACTTACC TGGACTGGGT CATGATCATC GTAACCATCT GCTCTTGCAT TTCCATGATG 
TTTGAGTCCC CGTTTCGAAG AGTCATGCAT GCACCTACTT TGCAGATTGC TGAGTATGTG 
TTTGTGATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTATTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTCGGT GGAGTAATGG ACATATTTAT ATATCTTGTG 
AGCTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CTGAATCGGG AGCTCAGCTT 
CTAATGGTCC TTCGGTGCCT GAGACCTCTG CGCATATTCA AACTGGTGCC CCAGATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGCTTC AAGGAAATTT TTTTGGTCTC CATTCTTTTG 
CTGACATTAA TGCTCGTTTT TGCAAGCTTT GGAGTTCAGC TTTTTGCTGG AAAACTGGCC 
AAGTGCAATG ATCCCAACAT TATTAGAAGG GAAGATTGCA ATGGCATATT CAGAATTAAT 
GTCAGTGTGT CAAAGAACTT AAATTTAAAA TTGAGGCCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC GAATCCTCGG AACTTTAATT TCGACAATGT GGGAAACGCT 
ATGCTGGCGT TGTTTGAAGT TCTCTCCTTG AAAGGCTGGG TGGAAGTGAG AGATGTTATT 
ATTCATCGTG TGGGGCCGAT CCATGGAATC TATATTCATG TTTTTGTATT CCTGGGTTGC 
ATGATTGGAC TGACCCTTTT TGTTGGAGTA GTTATTGCTA ATTTCAATGA AAACAAGGGG 
ACGGCTTTGC TGACCGTCGA TCAGAGAAGA TGGGAAGACC TGAAGAGCCG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC GCCTCGCCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATCGCAT TACTCGTCCT GGCCCAGTCG 
GTGTTGCTCT CTGTCAAGTG GGACGTCGAG GACCCGGTGA CCGTACCTTT GGCAACAATG 
TCAGTTGTTT TCACCTTCAT CTTTGTTCTG GAGGTTACCA TGAAGATCAT AGCAATGTCG 
CCTGCTGGCT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCCTGGTGAC ATCGCTTGGC 
GTTGTATGGG TGGTGCTTCA CTTTGCCCTC CTGAATGCAT ATACTTACAT GATGGGCGCT 
TGTGTGATTG TATTTAGGTT TTTCTCCATC TGTGGAAAAC ATGTAACGCT AAAGATGCTC 
CTCTTGACAG TGGTCGTCAG CATGTACAAG AGCTTCTTTA TCATAGTAGG CATGTTTCTC 
TTGCTGCTGT GTTACGCTTT TGCTGGAGTT GTTTTATTTG GTACTGTGAA ATATGGGGAG 
AATATTAACA GGCATGCAAA TTTTTCTTCG GCTGGAAAAG CTATTACCGT ACTGTTCCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGACT GTATGGTTCA GCCTCCGTTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GAAATTATGC TGGGGCACTT 
ATGTATTTCT GTTCATTTTA TGTCATCATT GCCTACATCA TGCTAAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC CTTGTTTTAT TCCACTGAGG AGGACCAGCT TTTAAGTTAC 
AATGATCTTC GCCACTTTCA AATCATATGG AACATGGTGG ATGATAAAAG AGAGGGGGTG 
ATCCCCACGT TCCGCGTCAA GTTCCTGCTG CGGCTACTGC GTGGGAGGCT GGAGGTGGAC 
CTGGACAAGG ACAAGCTCCT GTTTAAGCAC ATGTGCTACG AAATGGAGAG GCTCCACAAT 
GGCGGCGACG TCACCTTCCA TGATGTCCTG AGCATGCTTT CATACCGGTC CGTGGACATC 
CGGAAGAGCT TGCAGCTGGA GGAACTCCTG GCGAGGGAGC AGCTGGAGTA CACCATAGAG 
GAGGAGGTGG CCAAGCAGAC CATCCGCATG TGGCTCAAGA AGTGCCTGAA GCGCATCAGA 
GCTAAACAGC AGCAGTCGTG CAGTATCATC CACAGCCTGA GAGAGAGTCA GCAGCAAGAG 
CTGAGCCGGT TTCTGAACCC GCCCAGCATC GAGACCACCC AGCCCAGTGA GGACACGAAT 
GCCAACAGTC AGGACAACAG CATGCAACCT GAGACAAGCA GCCAGCAGCA GCTCCTGAGC 
CCCACGCTGT CGGATCGAGG AGGAAGTCGG CAAGATGCCG CCGATGCAGG GAAACCCCAG 
AGGAAATTTG GGCAGTGGCG TCTGCCCTCA GCCCCAAAAC CAATAAGCCA TTCAGTGTCC 
TCGGTCAACT TACGGTTTGG AGGAAGGACA ACCATGAAAT CTGTCGTGTG CAAAATGAAC 
CCCATGACTG ACGCGGCTTC CTGCGGTTCT GAAGTTAAGA AGTGGTGGAC CCGGCAGCTG 
ACTGTGGAGA GCGACGAAAG TGGGGATGAC CTTCTGGATA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009425465.1
CDS	197..5221
Translation	MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYVTFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWGMLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVNDTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_009427190.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009427190.3

ORF Insert Sequence:

ATGCTCAAAA GGAAGCAGAG TTCCAGGGTG GAAGCCCAGC CAGTCACTGA CTTTGGTCCT 
GATGAGTCTC TGTCGGATAA TGCTGACATC CTCTGGATTA ACAAACCATG GGTTCACTCT 
TTGCTGCGCA TCTGTGCCAT CATCAGCGTC ATTTCTGTTT GTATGAATAC GCCAATGACC 
TTCGAGCACT ATCCTCCACT TCAGTATGTG ACCTTCACTT TGGATACATT ATTGATGTTT 
CTCTACACGG CAGAGATGAT AGCAAAAATG CACATCCGGG GCATTGTCAA GGGGGATAGT 
TCCTATGTGA AAGATCGCTG GTGTGTTTTT GATGGATTTA TGGTCTTTTG CCTTTGGGTT 
TCTTTGGTGC TACAGGTGTT TGAAATTGCT GATATAGTTG ATCAGATGTC ACCTTGGGGC 
ATGTTGAGGA TTCCACGGCC ACTGATTATG ATCCGAGCAT TCCGGATTTA TTTCCGATTT 
GAACTGCCAA GGACCAGAAT TACAAATATT TTAAAGCGAT CCGGAGAACA AATATGGAGT 
GTTTCCATTT TTCTACTTTT CTTTCTACTT CTTTATGGAA TTTTAGGAGT TCAGATGTTT 
GGAACATTTA CTTATCACTG TGTTGTAAAC GACACAAAAC CAGGGAATGT AACCTGGAAT 
AGTTTAGCTA TTCCAGACAC ACACTGCTCA CCAGAGCTAG AAGAAGGCTA CCAGTGCCCA 
CCTGGATTTA AATGCATGGA CCTTGAAGAT CTGGGACTTA GCAGGCAAGA GCTGGGCTAC 
AGTGGCTTTA ATGAGATAGG AACCAGTATA TTCACCGTCT ATGAGGCCGC CTCGCAGGAA 
GGCTGGGTGT TCCTCATGTA CAGAGCAATT GACAGCTTTC CCCGTTGGCG TTCCTACTTC 
TATTTCATCA CTCTCATTTT CTTCCTCGCC TGGCTTGTGA AGAACGTGTT TATTGCTGTT 
ATCATTGAAA CATTTGCAGA AATCAGAGTA CAGTTTCAAC AAATGTGGGG ATCGAGAAGC 
AGCACTACCT CAACAGCCAC CACCCAGATG TTTCATGAAG ATGCTGCTGG AGGTTGGCAG 
CTGGTAGCTG TGGATGTCAA CAAGCCCCAG GGACGCGCCC CAGCCTGCCT CCAGAAAATG 
ATGCGGTCAT CCGTTTTCCA CATGTTCATC CTGAGCATGG TGACCGTGGA CGTGATCGTG 
GCGGCTAGCA ACTACTACAA AGGAGAAAAC TTCAGGAGGC AGTACGACGA GTTCTACCTG 
GCGGAGGTGG CTTTTACAGT ACTTTTTGAT TTGGAAGCAC TTCTGAAGAT ATGGTGTTTG 
GGATTTACTG GATATATTAG CTCATCTCTC CACAAATTCG AACTACTACT TGTAATTGGA 
ACTACTCTTC ATGTATATCC AGATCTTTAT CATTCACAAT TCACGTACTT TCAGGTTCTC 
CGAGTAGTTC GGCTGATTAA GATTTCACCT GCATTAGAAG ACTTTGTGTA CAAGATATTT 
GGTCCTGGAA AAAAGCTTGG GAGTTTGGTT GTATTTACTG CCAGCCTCTT GATTGTTATG 
TCAGCAATTA GTTTGCAGAT GTTCTGCTTT GTCGAAGAAC TGGACAGATT TACTACGTTT 
CCGAGGGCAT TTATGTCCAT GTTCCAGATC CTCACCCAGG AAGGATGGGT GGACGTAATG 
GACCAAACTC TAAATGCTGT GGGACATATG TGGGCACCCG TGGTTGCCAT CTATTTCATT 
CTCTATCATC TTTTTGCCAC TCTGATCCTC CTGAGTTTGT TTGTTGCTGT TATTTTGGAC 
AACTTAGAAC TTGATGAAGA CCTAAAGAAG CTTAAACAAC TTCGCAAGTC TGTTTTCAGC 
ATCAGGGCAA GGAACCTTCT GGAAAAGGAG ACCGCAGTCA CTAAAATCTT AAGCAAGAGG 
CCATTACCGA CGAGCTCACC CATTAGCATC CGCTGTTGTC TCTACTTGGG AAGATCACAT 
TGTCACTCTA GTGGAGTCAG AGCTTGCACC CGACAGCGCA TGCTGAGCGG ATCATTCGAG 
GGGCAGCCCG CAAAGGAGAG GTCAATCCTC AGCGTGCAGC ATCATATCCG CCAAGAGCGC 
AGGTCACTAA GACATGGATC GAACAGCCAG AGGATCAGCA GGGGAAAATC TCTTGAAACT 
TTGACTCAAG ATCATTCCAA TACAGTGAGA TATAGAAATG CACAAAGAGA AGACAGTGAA 
ATAAAGATGA TTCAGGAAAA AAAGGAGCAA GCAGAGATGA AAAGGAAAGT GCAAGAAGAG 
GAACTCAGAG AGAACCACCC ATACTTCGAT AAGCCACTGT TCATTGTCGG GCGAGAACAC 
AGGTTCAGAA ACTTTTGCCG GGTGGTGGTC CGAGCACGCT TCAACGCATC TAAAACAGAC 
CCTGTCACAG GAGCTGTGAA AAATACAAAG TACCATCAAC TTTATGATTT GCTGGGATTG 
GTCACTTACC TGGACTGGGT CATGATCATC GTAACCATCT GCTCTTGCAT TTCCATGATG 
TTTGAGTCCC CGTTTCGAAG AGTCATGCAT GCACCTACTT TGCAGATTGC TGAGTATGTG 
TTTGTGATAT TCATGAGCAT TGAGCTTAAT CTGAAGATTA TGGCAGATGG CTTATTTTTC 
ACTCCAACTG CTGTCATCAG GGACTTCGGT GGAGTAATGG ACATATTTAT ATATCTTGTG 
AGCTTGATAT TTCTTTGTTG GATGCCTCAA AATGTACCTG CTGAATCGGG AGCTCAGCTT 
CTAATGGTCC TTCGGTGCCT GAGACCTCTG CGCATATTCA AACTGGTGCC CCAGATGAGG 
AAAGTTGTTC GAGAACTTTT CAGTGGCTTC AAGGAAATTT TTTTGGTCTC CATTCTTTTG 
CTGACATTAA TGCTCGTTTT TGCAAGCTTT GGAGTTCAGC TTTTTGCTGG AAAACTGGCC 
AAGTGCAATG ATCCCAACAT TATTAGAAGG GAAGATTGCA ATGGCATATT CAGAATTAAT 
GTCAGTGTGT CAAAGAACTT AAATTTAAAA TTGAGGCCTG GAGAGAAAAA ACCTGGATTT 
TGGGTGCCCC GTGTTTGGGC GAATCCTCGG AACTTTAATT TCGACAATGT GGGAAACGCT 
ATGCTGGCGT TGTTTGAAGT TCTCTCCTTG AAAGGCTGGG TGGAAGTGAG AGATGTTATT 
ATTCATCGTG TGGGGCCGAT CCATGGAATC TATATTCATG TTTTTGTATT CCTGGGTTGC 
ATGATTGGAC TGACCCTTTT TGTTGGAGTA GTTATTGCTA ATTTCAATGA AAACAAGGGG 
ACGGCTTTGC TGACCGTCGA TCAGAGAAGA TGGGAAGACC TGAAGAGCCG ACTGAAGATC 
GCACAGCCTC TTCATCTCCC GCCTCGCCCG GATAATGATG GTTTTAGAGC TAAAATGTAT 
GACATAACCC AGCATCCATT TTTTAAGAGG ACAATCGCAT TACTCGTCCT GGCCCAGTCG 
GTGTTGCTCT CTGTCAAGTG GGACGTCGAG GACCCGGTGA CCGTACCTTT GGCAACAATG 
TCAGTTGTTT TCACCTTCAT CTTTGTTCTG GAGGTTACCA TGAAGATCAT AGCAATGTCG 
CCTGCTGGCT TCTGGCAAAG CAGAAGAAAC CGATATGATC TCCTGGTGAC ATCGCTTGGC 
GTTGTATGGG TGGTGCTTCA CTTTGCCCTC CTGAATGCAT ATACTTACAT GATGGGCGCT 
TGTGTGATTG TATTTAGGTT TTTCTCCATC TGTGGAAAAC ATGTAACGCT AAAGATGCTC 
CTCTTGACAG TGGTCGTCAG CATGTACAAG AGCTTCTTTA TCATAGTAGG CATGTTTCTC 
TTGCTGCTGT GTTACGCTTT TGCTGGAGTT GTTTTATTTG GTACTGTGAA ATATGGGGAG 
AATATTAACA GGCATGCAAA TTTTTCTTCG GCTGGAAAAG CTATTACCGT ACTGTTCCGA 
ATTGTCACAG GTGAAGACTG GAACAAGATT ATGCATGACT GTATGGTTCA GCCTCCGTTT 
TGTACTCCAG ATGAATTTAC ATACTGGGCA ACAGACTGTG GAAATTATGC TGGGGCACTT 
ATGTATTTCT GTTCATTTTA TGTCATCATT GCCTACATCA TGCTAAATCT GCTTGTAGCC 
ATAATTGTGG AGAATTTCTC CTTGTTTTAT TCCACTGAGG AGGACCAGCT TTTAAGTTAC 
AATGATCTTC GCCACTTTCA AATCATATGG AACATGGTGG ATGATAAAAG AGAGGGGGTG 
ATCCCCACGT TCCGCGTCAA GTTCCTGCTG CGGCTACTGC GTGGGAGGCT GGAGGTGGAC 
CTGGACAAGG ACAAGCTCCT GTTTAAGCAC ATGTGCTACG AAATGGAGAG GCTCCACAAT 
GGCGGCGACG TCACCTTCCA TGATGTCCTG AGCATGCTTT CATACCGGTC CGTGGACATC 
CGGAAGAGCT TGCAGCTGGA GGAACTCCTG GCGAGGGAGC AGCTGGAGTA CACCATAGAG 
GAGGAGGTGG CCAAGCAGAC CATCCGCATG TGGCTCAAGA AGTGCCTGAA GCGCATCAGA 
GCTAAACAGC AGCAGTCGTG CAGTATCATC CACAGCCTGA GAGAGAGTCA GCAGCAAGAG 
CTGAGCCGGT TTCTGAACCC GCCCAGCATC GAGACCACCC AGCCCAGTGA GGACACGAAT 
GCCAACAGTC AGGACAACAG CATGCAACCT GAGACAAGCA GCCAGCAGCA GCTCCTGAGC 
CCCACGCTGT CGGATCGAGG AGGAAGTCGG CAAGATGCCG CCGATGCAGG GAAACCCCAG 
AGGAAATTTG GGCAGTGGCG TCTGCCCTCA GCCCCAAAAC CAATAAGCCA TTCAGTGTCC 
TCGGTCAACT TACGGTTTGG AGGAAGGACA ACCATGAAAT CTGTCGTGTG CAAAATGAAC 
CCCATGACTG ACGCGGCTTC CTGCGGTTCT GAAGTTAAGA AGTGGTGGAC CCGGCAGCTG 
ACTGTGGAGA GCGACGAAAG TGGGGATGAC CTTCTGGATA TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NALCN ( XM_024348326.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024204094.1 Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X7, mRNA.

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CloneID	OPc96898
Clone ID Related Accession (Same CDS sequence)	XM_024348326.1
Accession Version	XM_024348326.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4539bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sodium leak channel non-selective protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036892.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACCC TGCATTGGTT GATGAACAAG AACAGAACCA GTATATTCAC CGTCTATGAG 
GCCGCCTCGC AGGAAGGCTG GGTGTTCCTC ATGTACAGAG CAATTGACAG CTTTCCCCGT 
TGGCGTTCCT ACTTCTATTT CATCACTCTC ATTTTCTTCC TCGCCTGGCT TGTGAAGAAC 
GTGTTTATTG CTGTTATCAT TGAAACATTT GCAGAAATCA GAGTACAGTT TCAACAAATG 
TGGGGATCGA GAAGCAGCAC TACCTCAACA GCCACCACCC AGATGTTTCA TGAAGATGCT 
GCTGGAGGTT GGCAGCTGGT AGCTGTGGAT GTCAACAAGC CCCAGGGACG CGCCCCAGCC 
TGCCTCCAGA AAATGATGCG GTCATCCGTT TTCCACATGT TCATCCTGAG CATGGTGACC 
GTGGACGTGA TCGTGGCGGC TAGCAACTAC TACAAAGGAG AAAACTTCAG GAGGCAGTAC 
GACGAGTTCT ACCTGGCGGA GGTGGCTTTT ACAGTACTTT TTGATTTGGA AGCACTTCTG 
AAGATATGGT GTTTGGGATT TACTGGATAT ATTAGCTCAT CTCTCCACAA ATTCGAACTA 
CTACTTGTAA TTGGAACTAC TCTTCATGTA TATCCAGATC TTTATCATTC ACAATTCACG 
TACTTTCAGG TTCTCCGAGT AGTTCGGCTG ATTAAGATTT CACCTGCATT AGAAGACTTT 
GTGTACAAGA TATTTGGTCC TGGAAAAAAG CTTGGGAGTT TGGTTGTATT TACTGCCAGC 
CTCTTGATTG TTATGTCAGC AATTAGTTTG CAGATGTTCT GCTTTGTCGA AGAACTGGAC 
AGATTTACTA CGTTTCCGAG GGCATTTATG TCCATGTTCC AGATCCTCAC CCAGGAAGGA 
TGGGTGGACG TAATGGACCA AACTCTAAAT GCTGTGGGAC ATATGTGGGC ACCCGTGGTT 
GCCATCTATT TCATTCTCTA TCATCTTTTT GCCACTCTGA TCCTCCTGAG TTTGTTTGTT 
GCTGTTATTT TGGACAACTT AGAACTTGAT GAAGACCTAA AGAAGCTTAA ACAATTAAAG 
CAAAGTGAAG CAAATGCGGA CACCAAAGAA AAGCTCCCTT TACGCCTGCG AATCTTTGAA 
AAATTTCCAA ACAGACCTCA AATGGTGAAA ATCTCAAAGC TTCCTTCAGA TTTTACAGTT 
CCTAAAATCA GGGAGAGTTT TATGAAGCAG TTTATTGACC GCCAGCAACA GGACACATGT 
TGCCTCCTGA GAAGCCTCCC GACCACCTCT TCCTCCTCCT GCGACCACTC CAAACGCTCG 
GCAATTGAGG ACAACAAATA CATCGACCAA AAACTTCGCA AGTCTGTTTT CAGCATCAGG 
GCAAGGAACC TTCTGGAAAA GGAGACCGCA GTCACTAAAA TCTTAAGCAA GAGGCCATTA 
CCGACGAGCT CACCCATTAG CATCCGCTGT TGTCTCTACT TGGGAAGATC ACATTGTCAC 
TCTAGTGGAG TCAGAGCTTG CACCCGACAG CGCATGCTGA GCGGATCATT CGAGGGGCAG 
CCCGCAAAGG AGAGGTCAAT CCTCAGCGTG CAGCATCATA TCCGCCAAGA GCGCAGGTCA 
CTAAGACATG GATCGAACAG CCAGAGGATC AGCAGGGGAA AATCTCTTGA AACTTTGACT 
CAAGATCATT CCAATACAGT GAGATATAGA AATGCACAAA GAGAAGACAG TGAAATAAAG 
ATGATTCAGG AAAAAAAGGA GCAAGCAGAG ATGAAAAGGA AAGTGCAAGA AGAGGAACTC 
AGAGAGAACC ACCCATACTT CGATAAGCCA CTGTTCATTG TCGGGCGAGA ACACAGGTTC 
AGAAACTTTT GCCGGGTGGT GGTCCGAGCA CGCTTCAACG CATCTAAAAC AGACCCTGTC 
ACAGGAGCTG TGAAAAATAC AAAGTACCAT CAACTTTATG ATTTGCTGGG ATTGGTCACT 
TACCTGGACT GGGTCATGAT CATCGTAACC ATCTGCTCTT GCATTTCCAT GATGTTTGAG 
TCCCCGTTTC GAAGAGTCAT GCATGCACCT ACTTTGCAGA TTGCTGAGTA TGTGTTTGTG 
ATATTCATGA GCATTGAGCT TAATCTGAAG ATTATGGCAG ATGGCTTATT TTTCACTCCA 
ACTGCTGTCA TCAGGGACTT CGGTGGAGTA ATGGACATAT TTATATATCT TGTGAGCTTG 
ATATTTCTTT GTTGGATGCC TCAAAATGTA CCTGCTGAAT CGGGAGCTCA GCTTCTAATG 
GTCCTTCGGT GCCTGAGACC TCTGCGCATA TTCAAACTGG TGCCCCAGAT GAGGAAAGTT 
GTTCGAGAAC TTTTCAGTGG CTTCAAGGAA ATTTTTTTGG TCTCCATTCT TTTGCTGACA 
TTAATGCTCG TTTTTGCAAG CTTTGGAGTT CAGCTTTTTG CTGGAAAACT GGCCAAGTGC 
AATGATCCCA ACATTATTAG AAGGGAAGAT TGCAATGGCA TATTCAGAAT TAATGTCAGT 
GTGTCAAAGA ACTTAAATTT AAAATTGAGG CCTGGAGAGA AAAAACCTGG ATTTTGGGTG 
CCCCGTGTTT GGGCGAATCC TCGGAACTTT AATTTCGACA ATGTGGGAAA CGCTATGCTG 
GCGTTGTTTG AAGTTCTCTC CTTGAAAGGC TGGGTGGAAG TGAGAGATGT TATTATTCAT 
CGTGTGGGGC CGATCCATGG AATCTATATT CATGTTTTTG TATTCCTGGG TTGCATGATT 
GGACTGACCC TTTTTGTTGG AGTAGTTATT GCTAATTTCA ATGAAAACAA GGGGACGGCT 
TTGCTGACCG TCGATCAGAG AAGATGGGAA GACCTGAAGA GCCGACTGAA GATCGCACAG 
CCTCTTCATC TCCCGCCTCG CCCGGATAAT GATGGTTTTA GAGCTAAAAT GTATGACATA 
ACCCAGCATC CATTTTTTAA GAGGACAATC GCATTACTCG TCCTGGCCCA GTCGGTGTTG 
CTCTCTGTCA AGTGGGACGT CGAGGACCCG GTGACCGTAC CTTTGGCAAC AATGTCAGTT 
GTTTTCACCT TCATCTTTGT TCTGGAGGTT ACCATGAAGA TCATAGCAAT GTCGCCTGCT 
GGCTTCTGGC AAAGCAGAAG AAACCGATAT GATCTCCTGG TGACATCGCT TGGCGTTGTA 
TGGGTGGTGC TTCACTTTGC CCTCCTGAAT GCATATACTT ACATGATGGG CGCTTGTGTG 
ATTGTATTTA GGTTTTTCTC CATCTGTGGA AAACATGTAA CGCTAAAGAT GCTCCTCTTG 
ACAGTGGTCG TCAGCATGTA CAAGAGCTTC TTTATCATAG TAGGCATGTT TCTCTTGCTG 
CTGTGTTACG CTTTTGCTGG AGTTGTTTTA TTTGGTACTG TGAAATATGG GGAGAATATT 
AACAGGCATG CAAATTTTTC TTCGGCTGGA AAAGCTATTA CCGTACTGTT CCGAATTGTC 
ACAGGTGAAG ACTGGAACAA GATTATGCAT GACTGTATGG TTCAGCCTCC GTTTTGTACT 
CCAGATGAAT TTACATACTG GGCAACAGAC TGTGGAAATT ATGCTGGGGC ACTTATGTAT 
TTCTGTTCAT TTTATGTCAT CATTGCCTAC ATCATGCTAA ATCTGCTTGT AGCCATAATT 
GTGGAGAATT TCTCCTTGTT TTATTCCACT GAGGAGGACC AGCTTTTAAG TTACAATGAT 
CTTCGCCACT TTCAAATCAT ATGGAACATG GTGGATGATA AAAGAGAGGG GGTGATCCCC 
ACGTTCCGCG TCAAGTTCCT GCTGCGGCTA CTGCGTGGGA GGCTGGAGGT GGACCTGGAC 
AAGGACAAGC TCCTGTTTAA GCACATGTGC TACGAAATGG AGAGGCTCCA CAATGGCGGC 
GACGTCACCT TCCATGATGT CCTGAGCATG CTTTCATACC GGTCCGTGGA CATCCGGAAG 
AGCTTGCAGC TGGAGGAACT CCTGGCGAGG GAGCAGCTGG AGTACACCAT AGAGGAGGAG 
GTGGCCAAGC AGACCATCCG CATGTGGCTC AAGAAGTGCC TGAAGCGCAT CAGAGCTAAA 
CAGCAGCAGT CGTGCAGTAT CATCCACAGC CTGAGAGAGA GTCAGCAGCA AGAGCTGAGC 
CGGTTTCTGA ACCCGCCCAG CATCGAGACC ACCCAGCCCA GTGAGGACAC GAATGCCAAC 
AGTCAGGACA ACAGCATGCA ACCTGAGACA AGCAGCCAGC AGCAGCTCCT GAGCCCCACG 
CTGTCGGATC GAGGAGGAAG TCGGCAAGAT GCCGCCGATG CAGGGAAACC CCAGAGGAAA 
TTTGGGCAGT GGCGTCTGCC CTCAGCCCCA AAACCAATAA GCCATTCAGT GTCCTCGGTC 
AACTTACGGT TTGGAGGAAG GACAACCATG AAATCTGTCG TGTGCAAAAT GAACCCCATG 
ACTGACGCGG CTTCCTGCGG TTCTGAAGTT AAGAAGTGGT GGACCCGGCA GCTGACTGTG 
GAGAGCGACG AAAGTGGGGA TGACCTTCTG GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024204094.1
CDS	118..4656
Translation	MNTLHWLMNKNRTSIFTVYEAASQEGWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGSRSSTTSTATTQMFHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYLAEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISPALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVMDQTLNAVGHMWAPVVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLRLRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLLRSLPTTSSSSCDHSKRSAIEDNKYIDQKLRKSVFSIRARNLLEKETAVTKILSKRPLPTSSPISIRCCLYLGRSHCHSSGVRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHGSNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIVGREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMIIVTICSCISMMFESPFRRVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAESGAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPNIIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEVRDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHLPPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVEDPVTVPLATMSVVFTFIFVLEVTMKIIAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVVSMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMVQPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHFQIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYRSVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQQQELSRFLNPPSIETTQPSEDTNANSQDNSMQPETSSQQQLLSPTLSDRGGSRQDAADAGKPQRKFGQWRLPSAPKPISHSVSSVNLRFGGRTTMKSVVCKMNPMTDAASCGSEVKKWWTRQLTVESDESGDDLLDI

Target ORF information:

RefSeq Version	XM_024348326.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sodium leak channel, non-selective (NALCN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348326.1

ORF Insert Sequence:

ATGAATACCC TGCATTGGTT GATGAACAAG AACAGAACCA GTATATTCAC CGTCTATGAG 
GCCGCCTCGC AGGAAGGCTG GGTGTTCCTC ATGTACAGAG CAATTGACAG CTTTCCCCGT 
TGGCGTTCCT ACTTCTATTT CATCACTCTC ATTTTCTTCC TCGCCTGGCT TGTGAAGAAC 
GTGTTTATTG CTGTTATCAT TGAAACATTT GCAGAAATCA GAGTACAGTT TCAACAAATG 
TGGGGATCGA GAAGCAGCAC TACCTCAACA GCCACCACCC AGATGTTTCA TGAAGATGCT 
GCTGGAGGTT GGCAGCTGGT AGCTGTGGAT GTCAACAAGC CCCAGGGACG CGCCCCAGCC 
TGCCTCCAGA AAATGATGCG GTCATCCGTT TTCCACATGT TCATCCTGAG CATGGTGACC 
GTGGACGTGA TCGTGGCGGC TAGCAACTAC TACAAAGGAG AAAACTTCAG GAGGCAGTAC 
GACGAGTTCT ACCTGGCGGA GGTGGCTTTT ACAGTACTTT TTGATTTGGA AGCACTTCTG 
AAGATATGGT GTTTGGGATT TACTGGATAT ATTAGCTCAT CTCTCCACAA ATTCGAACTA 
CTACTTGTAA TTGGAACTAC TCTTCATGTA TATCCAGATC TTTATCATTC ACAATTCACG 
TACTTTCAGG TTCTCCGAGT AGTTCGGCTG ATTAAGATTT CACCTGCATT AGAAGACTTT 
GTGTACAAGA TATTTGGTCC TGGAAAAAAG CTTGGGAGTT TGGTTGTATT TACTGCCAGC 
CTCTTGATTG TTATGTCAGC AATTAGTTTG CAGATGTTCT GCTTTGTCGA AGAACTGGAC 
AGATTTACTA CGTTTCCGAG GGCATTTATG TCCATGTTCC AGATCCTCAC CCAGGAAGGA 
TGGGTGGACG TAATGGACCA AACTCTAAAT GCTGTGGGAC ATATGTGGGC ACCCGTGGTT 
GCCATCTATT TCATTCTCTA TCATCTTTTT GCCACTCTGA TCCTCCTGAG TTTGTTTGTT 
GCTGTTATTT TGGACAACTT AGAACTTGAT GAAGACCTAA AGAAGCTTAA ACAATTAAAG 
CAAAGTGAAG CAAATGCGGA CACCAAAGAA AAGCTCCCTT TACGCCTGCG AATCTTTGAA 
AAATTTCCAA ACAGACCTCA AATGGTGAAA ATCTCAAAGC TTCCTTCAGA TTTTACAGTT 
CCTAAAATCA GGGAGAGTTT TATGAAGCAG TTTATTGACC GCCAGCAACA GGACACATGT 
TGCCTCCTGA GAAGCCTCCC GACCACCTCT TCCTCCTCCT GCGACCACTC CAAACGCTCG 
GCAATTGAGG ACAACAAATA CATCGACCAA AAACTTCGCA AGTCTGTTTT CAGCATCAGG 
GCAAGGAACC TTCTGGAAAA GGAGACCGCA GTCACTAAAA TCTTAAGCAA GAGGCCATTA 
CCGACGAGCT CACCCATTAG CATCCGCTGT TGTCTCTACT TGGGAAGATC ACATTGTCAC 
TCTAGTGGAG TCAGAGCTTG CACCCGACAG CGCATGCTGA GCGGATCATT CGAGGGGCAG 
CCCGCAAAGG AGAGGTCAAT CCTCAGCGTG CAGCATCATA TCCGCCAAGA GCGCAGGTCA 
CTAAGACATG GATCGAACAG CCAGAGGATC AGCAGGGGAA AATCTCTTGA AACTTTGACT 
CAAGATCATT CCAATACAGT GAGATATAGA AATGCACAAA GAGAAGACAG TGAAATAAAG 
ATGATTCAGG AAAAAAAGGA GCAAGCAGAG ATGAAAAGGA AAGTGCAAGA AGAGGAACTC 
AGAGAGAACC ACCCATACTT CGATAAGCCA CTGTTCATTG TCGGGCGAGA ACACAGGTTC 
AGAAACTTTT GCCGGGTGGT GGTCCGAGCA CGCTTCAACG CATCTAAAAC AGACCCTGTC 
ACAGGAGCTG TGAAAAATAC AAAGTACCAT CAACTTTATG ATTTGCTGGG ATTGGTCACT 
TACCTGGACT GGGTCATGAT CATCGTAACC ATCTGCTCTT GCATTTCCAT GATGTTTGAG 
TCCCCGTTTC GAAGAGTCAT GCATGCACCT ACTTTGCAGA TTGCTGAGTA TGTGTTTGTG 
ATATTCATGA GCATTGAGCT TAATCTGAAG ATTATGGCAG ATGGCTTATT TTTCACTCCA 
ACTGCTGTCA TCAGGGACTT CGGTGGAGTA ATGGACATAT TTATATATCT TGTGAGCTTG 
ATATTTCTTT GTTGGATGCC TCAAAATGTA CCTGCTGAAT CGGGAGCTCA GCTTCTAATG 
GTCCTTCGGT GCCTGAGACC TCTGCGCATA TTCAAACTGG TGCCCCAGAT GAGGAAAGTT 
GTTCGAGAAC TTTTCAGTGG CTTCAAGGAA ATTTTTTTGG TCTCCATTCT TTTGCTGACA 
TTAATGCTCG TTTTTGCAAG CTTTGGAGTT CAGCTTTTTG CTGGAAAACT GGCCAAGTGC 
AATGATCCCA ACATTATTAG AAGGGAAGAT TGCAATGGCA TATTCAGAAT TAATGTCAGT 
GTGTCAAAGA ACTTAAATTT AAAATTGAGG CCTGGAGAGA AAAAACCTGG ATTTTGGGTG 
CCCCGTGTTT GGGCGAATCC TCGGAACTTT AATTTCGACA ATGTGGGAAA CGCTATGCTG 
GCGTTGTTTG AAGTTCTCTC CTTGAAAGGC TGGGTGGAAG TGAGAGATGT TATTATTCAT 
CGTGTGGGGC CGATCCATGG AATCTATATT CATGTTTTTG TATTCCTGGG TTGCATGATT 
GGACTGACCC TTTTTGTTGG AGTAGTTATT GCTAATTTCA ATGAAAACAA GGGGACGGCT 
TTGCTGACCG TCGATCAGAG AAGATGGGAA GACCTGAAGA GCCGACTGAA GATCGCACAG 
CCTCTTCATC TCCCGCCTCG CCCGGATAAT GATGGTTTTA GAGCTAAAAT GTATGACATA 
ACCCAGCATC CATTTTTTAA GAGGACAATC GCATTACTCG TCCTGGCCCA GTCGGTGTTG 
CTCTCTGTCA AGTGGGACGT CGAGGACCCG GTGACCGTAC CTTTGGCAAC AATGTCAGTT 
GTTTTCACCT TCATCTTTGT TCTGGAGGTT ACCATGAAGA TCATAGCAAT GTCGCCTGCT 
GGCTTCTGGC AAAGCAGAAG AAACCGATAT GATCTCCTGG TGACATCGCT TGGCGTTGTA 
TGGGTGGTGC TTCACTTTGC CCTCCTGAAT GCATATACTT ACATGATGGG CGCTTGTGTG 
ATTGTATTTA GGTTTTTCTC CATCTGTGGA AAACATGTAA CGCTAAAGAT GCTCCTCTTG 
ACAGTGGTCG TCAGCATGTA CAAGAGCTTC TTTATCATAG TAGGCATGTT TCTCTTGCTG 
CTGTGTTACG CTTTTGCTGG AGTTGTTTTA TTTGGTACTG TGAAATATGG GGAGAATATT 
AACAGGCATG CAAATTTTTC TTCGGCTGGA AAAGCTATTA CCGTACTGTT CCGAATTGTC 
ACAGGTGAAG ACTGGAACAA GATTATGCAT GACTGTATGG TTCAGCCTCC GTTTTGTACT 
CCAGATGAAT TTACATACTG GGCAACAGAC TGTGGAAATT ATGCTGGGGC ACTTATGTAT 
TTCTGTTCAT TTTATGTCAT CATTGCCTAC ATCATGCTAA ATCTGCTTGT AGCCATAATT 
GTGGAGAATT TCTCCTTGTT TTATTCCACT GAGGAGGACC AGCTTTTAAG TTACAATGAT 
CTTCGCCACT TTCAAATCAT ATGGAACATG GTGGATGATA AAAGAGAGGG GGTGATCCCC 
ACGTTCCGCG TCAAGTTCCT GCTGCGGCTA CTGCGTGGGA GGCTGGAGGT GGACCTGGAC 
AAGGACAAGC TCCTGTTTAA GCACATGTGC TACGAAATGG AGAGGCTCCA CAATGGCGGC 
GACGTCACCT TCCATGATGT CCTGAGCATG CTTTCATACC GGTCCGTGGA CATCCGGAAG 
AGCTTGCAGC TGGAGGAACT CCTGGCGAGG GAGCAGCTGG AGTACACCAT AGAGGAGGAG 
GTGGCCAAGC AGACCATCCG CATGTGGCTC AAGAAGTGCC TGAAGCGCAT CAGAGCTAAA 
CAGCAGCAGT CGTGCAGTAT CATCCACAGC CTGAGAGAGA GTCAGCAGCA AGAGCTGAGC 
CGGTTTCTGA ACCCGCCCAG CATCGAGACC ACCCAGCCCA GTGAGGACAC GAATGCCAAC 
AGTCAGGACA ACAGCATGCA ACCTGAGACA AGCAGCCAGC AGCAGCTCCT GAGCCCCACG 
CTGTCGGATC GAGGAGGAAG TCGGCAAGAT GCCGCCGATG CAGGGAAACC CCAGAGGAAA 
TTTGGGCAGT GGCGTCTGCC CTCAGCCCCA AAACCAATAA GCCATTCAGT GTCCTCGGTC 
AACTTACGGT TTGGAGGAAG GACAACCATG AAATCTGTCG TGTGCAAAAT GAACCCCATG 
ACTGACGCGG CTTCCTGCGG TTCTGAAGTT AAGAAGTGGT GGACCCGGCA GCTGACTGTG 
GAGAGCGACG AAAGTGGGGA TGACCTTCTG GATATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NALCN
Entrez Gene ID	452643
Full Name	sodium leak channel, non-selective
Synonyms	VGCNL1
General protein information	Preferred Names sodium leak channel, non-selective Names sodium leak channel non-selective protein voltage gated channel like 1
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Pan troglodytes (chimpanzee)	NALCN	XP_001149573.1
Canis lupus familiaris (dog)	NALCN	XP_542654.4
Bos taurus (cattle)	NALCN	XP_002692026.1
Rattus norvegicus (Norway rat)	Nalcn	NP_705894.1
Drosophila melanogaster (fruit fly)	na	NP_001096981.1
Homo sapiens (human)	NALCN	NP_443099.1
Caenorhabditis elegans (roundworm)	nca-2	NP_498054.2
Mus musculus (house mouse)	Nalcn	NP_796367.3
Caenorhabditis elegans (roundworm)	unc-77	NP_741413.2
Gallus gallus (chicken)	NALCN	XP_416967.2
Danio rerio (zebrafish)	nalcn	NP_001017549.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003921	XP_318373.5
Xenopus tropicalis (tropical clawed frog)	nalcn	XP_002937125.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NALCN cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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