PHKA1 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_004940601.2	XP_004940658.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
XM_004940599.2	XP_004940656.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_004940602.2	XP_004940659.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
XM_004940602.3	XP_004940659.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
XM_004940598.2	XP_004940655.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_004940600.2	XP_004940657.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_004940598.3	XP_004940655.3	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_004940599.3	XP_004940656.3	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_025150325.1	XP_025006093.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_004940600.3	XP_004940657.3	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
XM_004940601.3	XP_004940658.3	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa07301	XM_004940598.3 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa07302	XM_004940599.3 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa07304	XM_004940600.3 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa07305	XM_004940601.3 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa150585	XM_025150325.1 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa34665	XM_004940598.2 Latest version!	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34666	XM_004940599.2 Latest version!	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34667	XM_004940600.2 Latest version!	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa34668	XM_004940601.2 Latest version!	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa07307	XM_004940602.3 Latest version!	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OGa07307	XM_004940602.2	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

PHKA1 ( XM_004940598.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940655.3 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa07301
Clone ID Related Accession (Same CDS sequence)	XM_004940598.3
Accession Version	XM_004940598.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGCCTGGG GACGGTGACC TGCTGGCCTC TCTGTCTGCT 
GGGCAATCGG AGCTGCTGGA CAAAGGCTCC TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT 
AAGGACAGCC GCCAGGGCCA GTGGAAACGG AGACGGCGGC TGGATGGGGC CCTCAACCGT 
GTCCCCTTGG GTTTCTACCA GAAGGTCTGG AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC 
GTGGAGGGCT TTGTTCTCCC CTCTTCAACA ACCAGAGAGA TGACCCCAGG GGAAATGAAG 
TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG 
CTGGTAGAGA CGATCTTGGT GCTCACCATG CTGGTGGATG TGGAGGTGCA CACCATGGGT 
GGCATCATAC CTGTGGAAAA GATCCTGCAC GCAGCCAATG ACCTCTTCTA TGAGGAGCAG 
AAGGCCCTGG GTGCTGATGA CCAGATGCTG GAGAAGGATC CCTCGACGGG CATTTGCGAC 
CTGCTGTACG ACAGCGCACC GAGTGGCCGC TTCGGCACCA TGACCTACCT GTCCAAGTCA 
GTGGCCCTGC ACCTGTACGA CCTCCTGCCC GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940655.3
CDS	109..3822
Translation	MRGRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940598.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940598.3

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGCCTGGG GACGGTGACC TGCTGGCCTC TCTGTCTGCT 
GGGCAATCGG AGCTGCTGGA CAAAGGCTCC TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT 
AAGGACAGCC GCCAGGGCCA GTGGAAACGG AGACGGCGGC TGGATGGGGC CCTCAACCGT 
GTCCCCTTGG GTTTCTACCA GAAGGTCTGG AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC 
GTGGAGGGCT TTGTTCTCCC CTCTTCAACA ACCAGAGAGA TGACCCCAGG GGAAATGAAG 
TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG 
CTGGTAGAGA CGATCTTGGT GCTCACCATG CTGGTGGATG TGGAGGTGCA CACCATGGGT 
GGCATCATAC CTGTGGAAAA GATCCTGCAC GCAGCCAATG ACCTCTTCTA TGAGGAGCAG 
AAGGCCCTGG GTGCTGATGA CCAGATGCTG GAGAAGGATC CCTCGACGGG CATTTGCGAC 
CTGCTGTACG ACAGCGCACC GAGTGGCCGC TTCGGCACCA TGACCTACCT GTCCAAGTCA 
GTGGCCCTGC ACCTGTACGA CCTCCTGCCC GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940599.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940656.3 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa07302
Clone ID Related Accession (Same CDS sequence)	XM_004940599.3
Accession Version	XM_004940599.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940656.3
CDS	1..3675
Translation	MRGRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940599.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940599.3

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940600.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940657.3 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.

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CloneID	OGa07304
Clone ID Related Accession (Same CDS sequence)	XM_004940600.3
Accession Version	XM_004940600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGCGGACAC ATACGATGAG GTTGCACAGT ACCTAGACCA CCTGCTGCAG 
CGCACGGTTC CCCAGTCAAA CCTCCCTCCC ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC 
CGTGCTGCCG TCCACACCAC CCGTGACCTC ATGTCCCTGG CGTCCAAGGC CAAGGACCTT 
CAGGTCCAGA ATGTTGGGAT GTATGTCCCT AGCAAGATCT TCCAGGCATC CCAGCAGTCA 
GTAAAGTTGT TGAGCTCACC CCACCAGCAT GACCAGGAAG TCAAGAGCCA CGCACTCCAT 
GCGGAGATGA ACCTGCCCCG CGATGAGGCT GGCAATGTGG ACTGCAAGGC ACTGGTGGAC 
CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTCTACAT GCTCCACATC 
CTCAAGGGGC CCGAGTGGCA CACGGGGCTT GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG 
CTCCTCACTG AGCTGTACGT GCGGGTGGGG GCCACGCGCC AGTGGGCTCT GATCCGCTAC 
ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA GCTTTGGATG AGGCCTGCAC GGCCCTGTTG 
TCTCACCAGA AGCACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACCATCTCT 
GCCCCAATCC CCTACGAGGA GCTCGTTCGC CTCATTGATG AAGCCAGTGA GAAGAACCTC 
AGCGTGTCCA TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT CCGCACCCAG 
CCAGCGCTCT TCACCGATAT GTTTCGAATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CGCACTCCCT GCACTGCTCA GCTGGAGAAG CCACTGAGAA CCTGATGAAT 
CTCAGCCCAT CCAACGTGAA GAACCTCCTC TACCACATCC TCTCCGGCAA GGAATTTGGA 
GTGGAGAAGA GTGTGCGATC GGTGGACTCA TTGGTCACCA CTGCTATCTC CATCTACGAT 
GTGGAGGCTG CTGGAGCCAC CAAGACTGAG CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG 
ATCAAACAGC AACTGGATAA ACGTAGGCAG TCTCTGACCG GGAGTAAGCC TGGGGACGGT 
GACCTGCTGG CCTCTCTGTC TGCTGGGCAA TCGGAGCTGC TGGACAAAGG CTCCTTTTCA 
AGCTTAGTGA AAGACCAGAG CAGTAAGGAC AGCCGCCAGG GCCAGTGGAA ACGGAGACGG 
CGGCTGGATG GGGCCCTCAA CCGTGTCCCC TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC 
CTGCAGAAGT GCCACGGTCT TTCCGTGGAG GGCTTTGTTC TCCCCTCTTC AACAACCAGA 
GAGATGACCC CAGGGGAAAT GAAGTTTGCT GTGCACGTGG AGTCGGTCCT CAACCGTGTG 
CCCCAGCCAG AGTACCGGCA GCTGCTGGTA GAGACGATCT TGGTGCTCAC CATGCTGGTG 
GATGTGGAGG TGCACACCAT GGGTGGCATC ATACCTGTGG AAAAGATCCT GCACGCAGCC 
AATGACCTCT TCTATGAGGA GCAGAAGGCC CTGGGTGCTG ATGACCAGAT GCTGGAGAAG 
GATCCCTCGA CGGGCATTTG CGACCTGCTG TACGACAGCG CACCGAGTGG CCGCTTCGGC 
ACCATGACCT ACCTGTCCAA GTCAGTGGCC CTGCACCTGT ACGACCTCCT GCCCGGAGAT 
GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940657.3
CDS	1..3618
Translation	MRGRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940600.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940600.3

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGCGGACAC ATACGATGAG GTTGCACAGT ACCTAGACCA CCTGCTGCAG 
CGCACGGTTC CCCAGTCAAA CCTCCCTCCC ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC 
CGTGCTGCCG TCCACACCAC CCGTGACCTC ATGTCCCTGG CGTCCAAGGC CAAGGACCTT 
CAGGTCCAGA ATGTTGGGAT GTATGTCCCT AGCAAGATCT TCCAGGCATC CCAGCAGTCA 
GTAAAGTTGT TGAGCTCACC CCACCAGCAT GACCAGGAAG TCAAGAGCCA CGCACTCCAT 
GCGGAGATGA ACCTGCCCCG CGATGAGGCT GGCAATGTGG ACTGCAAGGC ACTGGTGGAC 
CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTCTACAT GCTCCACATC 
CTCAAGGGGC CCGAGTGGCA CACGGGGCTT GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG 
CTCCTCACTG AGCTGTACGT GCGGGTGGGG GCCACGCGCC AGTGGGCTCT GATCCGCTAC 
ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA GCTTTGGATG AGGCCTGCAC GGCCCTGTTG 
TCTCACCAGA AGCACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACCATCTCT 
GCCCCAATCC CCTACGAGGA GCTCGTTCGC CTCATTGATG AAGCCAGTGA GAAGAACCTC 
AGCGTGTCCA TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT CCGCACCCAG 
CCAGCGCTCT TCACCGATAT GTTTCGAATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CGCACTCCCT GCACTGCTCA GCTGGAGAAG CCACTGAGAA CCTGATGAAT 
CTCAGCCCAT CCAACGTGAA GAACCTCCTC TACCACATCC TCTCCGGCAA GGAATTTGGA 
GTGGAGAAGA GTGTGCGATC GGTGGACTCA TTGGTCACCA CTGCTATCTC CATCTACGAT 
GTGGAGGCTG CTGGAGCCAC CAAGACTGAG CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG 
ATCAAACAGC AACTGGATAA ACGTAGGCAG TCTCTGACCG GGAGTAAGCC TGGGGACGGT 
GACCTGCTGG CCTCTCTGTC TGCTGGGCAA TCGGAGCTGC TGGACAAAGG CTCCTTTTCA 
AGCTTAGTGA AAGACCAGAG CAGTAAGGAC AGCCGCCAGG GCCAGTGGAA ACGGAGACGG 
CGGCTGGATG GGGCCCTCAA CCGTGTCCCC TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC 
CTGCAGAAGT GCCACGGTCT TTCCGTGGAG GGCTTTGTTC TCCCCTCTTC AACAACCAGA 
GAGATGACCC CAGGGGAAAT GAAGTTTGCT GTGCACGTGG AGTCGGTCCT CAACCGTGTG 
CCCCAGCCAG AGTACCGGCA GCTGCTGGTA GAGACGATCT TGGTGCTCAC CATGCTGGTG 
GATGTGGAGG TGCACACCAT GGGTGGCATC ATACCTGTGG AAAAGATCCT GCACGCAGCC 
AATGACCTCT TCTATGAGGA GCAGAAGGCC CTGGGTGCTG ATGACCAGAT GCTGGAGAAG 
GATCCCTCGA CGGGCATTTG CGACCTGCTG TACGACAGCG CACCGAGTGG CCGCTTCGGC 
ACCATGACCT ACCTGTCCAA GTCAGTGGCC CTGCACCTGT ACGACCTCCT GCCCGGAGAT 
GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940601.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940658.3 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.

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CloneID	OGa07305
Clone ID Related Accession (Same CDS sequence)	XM_004940601.3
Accession Version	XM_004940601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGTC CATCCTCCAC TCGATGCTGC CGAGGGCCTC CTCGTCCAAG 
GAGGTGGATG CCAGTGTGCT CTCTGTCATC TCCTACCCAG CCTTTGCTGT GGAGGACAGC 
GAGCTGGTGG AAATTACCAA GCAAGAGATC ATCACAAAGC TGCAGGGGCG CTATGGCTGC 
TGCCGCTTCC TCCGTGACGG ATACAGGACC CCCAGAGAGG ATCCCAACCG CTCCTACTAT 
GAACCTGCAG AGCTGAAGCT CTTTGAGAAC ATCGAGTGTG AGTGGCCTTT GTTCTGGACA 
TACCTGATCA TTGATGGGAT TTTCAATGGA AACATGGAGC AGGTGCAAGA GTACCGGGAG 
GCCCTTGAGG GGATTCTCAT CAAGGGGAAG AACGGGGTGC GCCTGGTGCC TGAGCTGTAC 
AGTGTCCCAC CAGACAAGGT GGATGAGGAA TACAACAACC CCCACACTGT GGACAGGATA 
CCCATGGGCA AGCTGCCACT CATGTGGGGC CAGTCCCTCT ACATCCTGGG CTGCCTGATG 
GCTGAGGGAT TCCTGGCTCC TGGTGAAATC GATCCCCTCA ACCGCCGCTT TGCGACTGTC 
CCCAAGCCCG ACGTGGTGGT GCAAGTGTGC ATCCTAGCGG AGACAGAGGG GATCAAGGCT 
ATTCTGAAGA AGGAGGGCAT CCATGTGGAG ACTGTGGCCG ATGTTTACCC CATCAGAGTG 
CAGCCTGCTC GCATCCTCAG CCACATCTAC GCTCGCCTGG GTCGCAACAA GCAGATGAGC 
TTGACAGGAC GGCCCTACCG GCACATGGGT GTGATTGGGA CCTCCAAGCT CTACAACATT 
AGGAAGAACA TCTTTGCCTT CACCCCACAG TTCATAGACC AGCAGCAGTT CTACCTGGCA 
CTCGACAGCA AGATGATTGT GGAGATGCTG AGGACAGACC TCTCATACCT CTGCAGCCGC 
TGGAGGATGA CTGGACGGCC CACCATCACC TTCCCTGTCT CTCAGACCAT GCTCGATGAT 
ACGGGCAGCA GCATACACCC CACAGTGCTG GCAACACTAC GGAAGCTGCA GGACGGGTAT 
TTCGGTGGTG CAAGGATCCA GACCGGTAAG TTGTCGGAGT TCCTGACAAC GTCGTGCCGA 
ACGCACCTCA GCTTCATGGA CCCTGGGCCG GAGGGTAAGG TCTTTGCCAA CGGTTACAAG 
CTCGGCCTTG ACAGCTTTGA GGAGCTAGAC CCTGAGGAGT GGCTGCATGG CTTGCAGTTT 
GCAGAGGATG CGGACACATA CGATGAGGTT GCACAGTACC TAGACCACCT GCTGCAGCGC 
ACGGTTCCCC AGTCAAACCT CCCTCCCACT GCCCAGCAAG GAGGGCTGAA GCGCTTCCGT 
GCTGCCGTCC ACACCACCCG TGACCTCATG TCCCTGGCGT CCAAGGCCAA GGACCTTCAG 
GTCCAGAATG TTGGGATGTA TGTCCCTAGC AAGATCTTCC AGGCATCCCA GCAGTCAGTA 
AAGTTGTTGA GCTCACCCCA CCAGCATGAC CAGGAAGTCA AGAGCCACGC ACTCCATGCG 
GAGATGAACC TGCCCCGCGA TGAGGCTGGC AATGTGGACT GCAAGGCACT GGTGGACCAG 
CTGCGCATCT GCCCCACGCT GCAGGAGCAA GCAGACATCC TCTACATGCT CCACATCCTC 
AAGGGGCCCG AGTGGCACAC GGGGCTTGAG AGTGAGCCTG GCCCCACTGT GAAGGAGCTC 
CTCACTGAGC TGTACGTGCG GGTGGGGGCC ACGCGCCAGT GGGCTCTGAT CCGCTACATT 
TCTGGTATCC TCAAGAAAAA GGTGGAAGCT TTGGATGAGG CCTGCACGGC CCTGTTGTCT 
CACCAGAAGC ACTTGACAGT GGGGCTGCCC CCAGAGCCAC GTGAGAAGAC CATCTCTGCC 
CCAATCCCCT ACGAGGAGCT CGTTCGCCTC ATTGATGAAG CCAGTGAGAA GAACCTCAGC 
GTGTCCATCC TCACCCAGGA GATCATGGTG TACTTGGCCA TGTACATCCG CACCCAGCCA 
GCGCTCTTCA CCGATATGTT TCGAATCCGC ATTGGGCTCA TCATCCAGGT GATGGCAACA 
GAGCTGGCGC ACTCCCTGCA CTGCTCAGCT GGAGAAGCCA CTGAGAACCT GATGAATCTC 
AGCCCATCCA ACGTGAAGAA CCTCCTCTAC CACATCCTCT CCGGCAAGGA ATTTGGAGTG 
GAGAAGAGTG TGCGATCGGT GGACTCATTG GTCACCACTG CTATCTCCAT CTACGATGTG 
GAGGCTGCTG GAGCCACCAA GACTGAGCGT GCTGGCATCG TCAGGCTGAA AAGTGAGATC 
AAACAGCAAC TGGATAAACG TAGGCAGTCT CTGACCGGGA GTAAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940658.3
CDS	109..3723
Translation	MRGRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940601.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940601.3

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGTC CATCCTCCAC TCGATGCTGC CGAGGGCCTC CTCGTCCAAG 
GAGGTGGATG CCAGTGTGCT CTCTGTCATC TCCTACCCAG CCTTTGCTGT GGAGGACAGC 
GAGCTGGTGG AAATTACCAA GCAAGAGATC ATCACAAAGC TGCAGGGGCG CTATGGCTGC 
TGCCGCTTCC TCCGTGACGG ATACAGGACC CCCAGAGAGG ATCCCAACCG CTCCTACTAT 
GAACCTGCAG AGCTGAAGCT CTTTGAGAAC ATCGAGTGTG AGTGGCCTTT GTTCTGGACA 
TACCTGATCA TTGATGGGAT TTTCAATGGA AACATGGAGC AGGTGCAAGA GTACCGGGAG 
GCCCTTGAGG GGATTCTCAT CAAGGGGAAG AACGGGGTGC GCCTGGTGCC TGAGCTGTAC 
AGTGTCCCAC CAGACAAGGT GGATGAGGAA TACAACAACC CCCACACTGT GGACAGGATA 
CCCATGGGCA AGCTGCCACT CATGTGGGGC CAGTCCCTCT ACATCCTGGG CTGCCTGATG 
GCTGAGGGAT TCCTGGCTCC TGGTGAAATC GATCCCCTCA ACCGCCGCTT TGCGACTGTC 
CCCAAGCCCG ACGTGGTGGT GCAAGTGTGC ATCCTAGCGG AGACAGAGGG GATCAAGGCT 
ATTCTGAAGA AGGAGGGCAT CCATGTGGAG ACTGTGGCCG ATGTTTACCC CATCAGAGTG 
CAGCCTGCTC GCATCCTCAG CCACATCTAC GCTCGCCTGG GTCGCAACAA GCAGATGAGC 
TTGACAGGAC GGCCCTACCG GCACATGGGT GTGATTGGGA CCTCCAAGCT CTACAACATT 
AGGAAGAACA TCTTTGCCTT CACCCCACAG TTCATAGACC AGCAGCAGTT CTACCTGGCA 
CTCGACAGCA AGATGATTGT GGAGATGCTG AGGACAGACC TCTCATACCT CTGCAGCCGC 
TGGAGGATGA CTGGACGGCC CACCATCACC TTCCCTGTCT CTCAGACCAT GCTCGATGAT 
ACGGGCAGCA GCATACACCC CACAGTGCTG GCAACACTAC GGAAGCTGCA GGACGGGTAT 
TTCGGTGGTG CAAGGATCCA GACCGGTAAG TTGTCGGAGT TCCTGACAAC GTCGTGCCGA 
ACGCACCTCA GCTTCATGGA CCCTGGGCCG GAGGGTAAGG TCTTTGCCAA CGGTTACAAG 
CTCGGCCTTG ACAGCTTTGA GGAGCTAGAC CCTGAGGAGT GGCTGCATGG CTTGCAGTTT 
GCAGAGGATG CGGACACATA CGATGAGGTT GCACAGTACC TAGACCACCT GCTGCAGCGC 
ACGGTTCCCC AGTCAAACCT CCCTCCCACT GCCCAGCAAG GAGGGCTGAA GCGCTTCCGT 
GCTGCCGTCC ACACCACCCG TGACCTCATG TCCCTGGCGT CCAAGGCCAA GGACCTTCAG 
GTCCAGAATG TTGGGATGTA TGTCCCTAGC AAGATCTTCC AGGCATCCCA GCAGTCAGTA 
AAGTTGTTGA GCTCACCCCA CCAGCATGAC CAGGAAGTCA AGAGCCACGC ACTCCATGCG 
GAGATGAACC TGCCCCGCGA TGAGGCTGGC AATGTGGACT GCAAGGCACT GGTGGACCAG 
CTGCGCATCT GCCCCACGCT GCAGGAGCAA GCAGACATCC TCTACATGCT CCACATCCTC 
AAGGGGCCCG AGTGGCACAC GGGGCTTGAG AGTGAGCCTG GCCCCACTGT GAAGGAGCTC 
CTCACTGAGC TGTACGTGCG GGTGGGGGCC ACGCGCCAGT GGGCTCTGAT CCGCTACATT 
TCTGGTATCC TCAAGAAAAA GGTGGAAGCT TTGGATGAGG CCTGCACGGC CCTGTTGTCT 
CACCAGAAGC ACTTGACAGT GGGGCTGCCC CCAGAGCCAC GTGAGAAGAC CATCTCTGCC 
CCAATCCCCT ACGAGGAGCT CGTTCGCCTC ATTGATGAAG CCAGTGAGAA GAACCTCAGC 
GTGTCCATCC TCACCCAGGA GATCATGGTG TACTTGGCCA TGTACATCCG CACCCAGCCA 
GCGCTCTTCA CCGATATGTT TCGAATCCGC ATTGGGCTCA TCATCCAGGT GATGGCAACA 
GAGCTGGCGC ACTCCCTGCA CTGCTCAGCT GGAGAAGCCA CTGAGAACCT GATGAATCTC 
AGCCCATCCA ACGTGAAGAA CCTCCTCTAC CACATCCTCT CCGGCAAGGA ATTTGGAGTG 
GAGAAGAGTG TGCGATCGGT GGACTCATTG GTCACCACTG CTATCTCCAT CTACGATGTG 
GAGGCTGCTG GAGCCACCAA GACTGAGCGT GCTGGCATCG TCAGGCTGAA AAGTGAGATC 
AAACAGCAAC TGGATAAACG TAGGCAGTCT CTGACCGGGA GTAAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_025150325.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025006093.1 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa150585
Clone ID Related Accession (Same CDS sequence)	XM_025150325.1
Accession Version	XM_025150325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGTCTGCT GGGCAATCGG AGCTGCTGGA CAAAGGCTCC 
TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT AAGGACAGCC GCCAGGGCCA GTGGAAACGG 
AGACGGCGGC TGGATGGGGC CCTCAACCGT GTCCCCTTGG GTTTCTACCA GAAGGTCTGG 
AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC GTGGAGGGCT TTGTTCTCCC CTCTTCAACA 
ACCAGAGAGA TGACCCCAGG GGAAATGAAG TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC 
CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG CTGGTAGAGA CGATCTTGGT GCTCACCATG 
CTGGTGGATG TGGAGGTGCA CACCATGGGT GGCATCATAC CTGTGGAAAA GATCCTGCAC 
GCAGCCAATG ACCTCTTCTA TGAGGAGCAG AAGGCCCTGG GTGCTGATGA CCAGATGCTG 
GAGAAGGATC CCTCGACGGG CATTTGCGAC CTGCTGTACG ACAGCGCACC GAGTGGCCGC 
TTCGGCACCA TGACCTACCT GTCCAAGTCA GTGGCCCTGC ACCTGTACGA CCTCCTGCCC 
GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025006093.1
CDS	1..3684
Translation	MRGRSNSGVRLDGYGRLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_025150325.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025150325.1

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGGCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGTCTGCT GGGCAATCGG AGCTGCTGGA CAAAGGCTCC 
TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT AAGGACAGCC GCCAGGGCCA GTGGAAACGG 
AGACGGCGGC TGGATGGGGC CCTCAACCGT GTCCCCTTGG GTTTCTACCA GAAGGTCTGG 
AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC GTGGAGGGCT TTGTTCTCCC CTCTTCAACA 
ACCAGAGAGA TGACCCCAGG GGAAATGAAG TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC 
CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG CTGGTAGAGA CGATCTTGGT GCTCACCATG 
CTGGTGGATG TGGAGGTGCA CACCATGGGT GGCATCATAC CTGTGGAAAA GATCCTGCAC 
GCAGCCAATG ACCTCTTCTA TGAGGAGCAG AAGGCCCTGG GTGCTGATGA CCAGATGCTG 
GAGAAGGATC CCTCGACGGG CATTTGCGAC CTGCTGTACG ACAGCGCACC GAGTGGCCGC 
TTCGGCACCA TGACCTACCT GTCCAAGTCA GTGGCCCTGC ACCTGTACGA CCTCCTGCCC 
GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940598.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940655.2 Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

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CloneID	OGa34665
Clone ID Related Accession (Same CDS sequence)	XM_004940598.2
Accession Version	XM_004940598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGCCTGGG GACGGTGACC TGCTGGCCTC TCTGTCTGCT 
GGGCAATCGG AGCTGCTGGA CAAAGGCTCC TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT 
AAGGACAGCC GCCAGGGCCA GTGGAAACGG AGACGGCGGC TGGATGGGGC CCTCAACCGT 
GTCCCCTTGG GTTTCTACCA GAAGGTCTGG AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC 
GTGGAGGGCT TTGTTCTCCC CTCTTCAACA ACCAGAGAGA TGACCCCAGG GGAAATGAAG 
TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG 
CTGGTAGAGA CGATCTTGGT GCTCACCATG CTGGTGGATG TGGAGGTGCA CACCATGGGT 
GGCATCATAC CTGTGGAAAA GATCCTGCAC GCAGCCAATG ACCTCTTCTA TGAGGAGCAG 
AAGGCCCTGG GTGCTGATGA CCAGATGCTG GAGAAGGATC CCTCGACGGG CATTTGCGAC 
CTGCTGTACG ACAGCGCACC GAGTGGCCGC TTCGGCACCA TGACCTACCT GTCCAAGTCA 
GTGGCCCTGC ACCTGTACGA CCTCCTGCCC GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940655.2
CDS	109..3822
Translation	MRGRSNSGVRLDGYARLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940598.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940598.2

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCAACT GGATAAACGT 
AGGCAGTCTC TGACCGGGAG TAAGCCTGGG GACGGTGACC TGCTGGCCTC TCTGTCTGCT 
GGGCAATCGG AGCTGCTGGA CAAAGGCTCC TTTTCAAGCT TAGTGAAAGA CCAGAGCAGT 
AAGGACAGCC GCCAGGGCCA GTGGAAACGG AGACGGCGGC TGGATGGGGC CCTCAACCGT 
GTCCCCTTGG GTTTCTACCA GAAGGTCTGG AAGGTCCTGC AGAAGTGCCA CGGTCTTTCC 
GTGGAGGGCT TTGTTCTCCC CTCTTCAACA ACCAGAGAGA TGACCCCAGG GGAAATGAAG 
TTTGCTGTGC ACGTGGAGTC GGTCCTCAAC CGTGTGCCCC AGCCAGAGTA CCGGCAGCTG 
CTGGTAGAGA CGATCTTGGT GCTCACCATG CTGGTGGATG TGGAGGTGCA CACCATGGGT 
GGCATCATAC CTGTGGAAAA GATCCTGCAC GCAGCCAATG ACCTCTTCTA TGAGGAGCAG 
AAGGCCCTGG GTGCTGATGA CCAGATGCTG GAGAAGGATC CCTCGACGGG CATTTGCGAC 
CTGCTGTACG ACAGCGCACC GAGTGGCCGC TTCGGCACCA TGACCTACCT GTCCAAGTCA 
GTGGCCCTGC ACCTGTACGA CCTCCTGCCC GGAGATGGCT GTGCCATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940599.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940656.2 Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa34666
Clone ID Related Accession (Same CDS sequence)	XM_004940599.2
Accession Version	XM_004940599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940656.2
CDS	1..3675
Translation	MRGRSNSGVRLDGYARLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940599.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940599.2

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGGTAAGGT CTTTGCCAAC GGTTACAAGC TCGGCCTTGA CAGCTTTGAG 
GAGCTAGACC CTGAGGAGTG GCTGCATGGC TTGCAGTTTG CAGAGGATGC GGACACATAC 
GATGAGGTTG CACAGTACCT AGACCACCTG CTGCAGCGCA CGGTTCCCCA GTCAAACCTC 
CCTCCCACTG CCCAGCAAGG AGGGCTGAAG CGCTTCCGTG CTGCCGTCCA CACCACCCGT 
GACCTCATGT CCCTGGCGTC CAAGGCCAAG GACCTTCAGG TCCAGAATGT TGGGATGTAT 
GTCCCTAGCA AGATCTTCCA GGCATCCCAG CAGTCAGTAA AGTTGTTGAG CTCACCCCAC 
CAGCATGACC AGGAAGTCAA GAGCCACGCA CTCCATGCGG AGATGAACCT GCCCCGCGAT 
GAGGCTGGCA ATGTGGACTG CAAGGCACTG GTGGACCAGC TGCGCATCTG CCCCACGCTG 
CAGGAGCAAG CAGACATCCT CTACATGCTC CACATCCTCA AGGGGCCCGA GTGGCACACG 
GGGCTTGAGA GTGAGCCTGG CCCCACTGTG AAGGAGCTCC TCACTGAGCT GTACGTGCGG 
GTGGGGGCCA CGCGCCAGTG GGCTCTGATC CGCTACATTT CTGGTATCCT CAAGAAAAAG 
GTGGAAGCTT TGGATGAGGC CTGCACGGCC CTGTTGTCTC ACCAGAAGCA CTTGACAGTG 
GGGCTGCCCC CAGAGCCACG TGAGAAGACC ATCTCTGCCC CAATCCCCTA CGAGGAGCTC 
GTTCGCCTCA TTGATGAAGC CAGTGAGAAG AACCTCAGCG TGTCCATCCT CACCCAGGAG 
ATCATGGTGT ACTTGGCCAT GTACATCCGC ACCCAGCCAG CGCTCTTCAC CGATATGTTT 
CGAATCCGCA TTGGGCTCAT CATCCAGGTG ATGGCAACAG AGCTGGCGCA CTCCCTGCAC 
TGCTCAGCTG GAGAAGCCAC TGAGAACCTG ATGAATCTCA GCCCATCCAA CGTGAAGAAC 
CTCCTCTACC ACATCCTCTC CGGCAAGGAA TTTGGAGTGG AGAAGAGTGT GCGATCGGTG 
GACTCATTGG TCACCACTGC TATCTCCATC TACGATGTGG AGGCTGCTGG AGCCACCAAG 
ACTGAGCGTG CTGGCATCGT CAGGCTGAAA AGTGAGATCA AACAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940600.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940657.2 Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

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CloneID	OGa34667
Clone ID Related Accession (Same CDS sequence)	XM_004940600.2
Accession Version	XM_004940600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGCGGACAC ATACGATGAG GTTGCACAGT ACCTAGACCA CCTGCTGCAG 
CGCACGGTTC CCCAGTCAAA CCTCCCTCCC ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC 
CGTGCTGCCG TCCACACCAC CCGTGACCTC ATGTCCCTGG CGTCCAAGGC CAAGGACCTT 
CAGGTCCAGA ATGTTGGGAT GTATGTCCCT AGCAAGATCT TCCAGGCATC CCAGCAGTCA 
GTAAAGTTGT TGAGCTCACC CCACCAGCAT GACCAGGAAG TCAAGAGCCA CGCACTCCAT 
GCGGAGATGA ACCTGCCCCG CGATGAGGCT GGCAATGTGG ACTGCAAGGC ACTGGTGGAC 
CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTCTACAT GCTCCACATC 
CTCAAGGGGC CCGAGTGGCA CACGGGGCTT GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG 
CTCCTCACTG AGCTGTACGT GCGGGTGGGG GCCACGCGCC AGTGGGCTCT GATCCGCTAC 
ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA GCTTTGGATG AGGCCTGCAC GGCCCTGTTG 
TCTCACCAGA AGCACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACCATCTCT 
GCCCCAATCC CCTACGAGGA GCTCGTTCGC CTCATTGATG AAGCCAGTGA GAAGAACCTC 
AGCGTGTCCA TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT CCGCACCCAG 
CCAGCGCTCT TCACCGATAT GTTTCGAATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CGCACTCCCT GCACTGCTCA GCTGGAGAAG CCACTGAGAA CCTGATGAAT 
CTCAGCCCAT CCAACGTGAA GAACCTCCTC TACCACATCC TCTCCGGCAA GGAATTTGGA 
GTGGAGAAGA GTGTGCGATC GGTGGACTCA TTGGTCACCA CTGCTATCTC CATCTACGAT 
GTGGAGGCTG CTGGAGCCAC CAAGACTGAG CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG 
ATCAAACAGC AACTGGATAA ACGTAGGCAG TCTCTGACCG GGAGTAAGCC TGGGGACGGT 
GACCTGCTGG CCTCTCTGTC TGCTGGGCAA TCGGAGCTGC TGGACAAAGG CTCCTTTTCA 
AGCTTAGTGA AAGACCAGAG CAGTAAGGAC AGCCGCCAGG GCCAGTGGAA ACGGAGACGG 
CGGCTGGATG GGGCCCTCAA CCGTGTCCCC TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC 
CTGCAGAAGT GCCACGGTCT TTCCGTGGAG GGCTTTGTTC TCCCCTCTTC AACAACCAGA 
GAGATGACCC CAGGGGAAAT GAAGTTTGCT GTGCACGTGG AGTCGGTCCT CAACCGTGTG 
CCCCAGCCAG AGTACCGGCA GCTGCTGGTA GAGACGATCT TGGTGCTCAC CATGCTGGTG 
GATGTGGAGG TGCACACCAT GGGTGGCATC ATACCTGTGG AAAAGATCCT GCACGCAGCC 
AATGACCTCT TCTATGAGGA GCAGAAGGCC CTGGGTGCTG ATGACCAGAT GCTGGAGAAG 
GATCCCTCGA CGGGCATTTG CGACCTGCTG TACGACAGCG CACCGAGTGG CCGCTTCGGC 
ACCATGACCT ACCTGTCCAA GTCAGTGGCC CTGCACCTGT ACGACCTCCT GCCCGGAGAT 
GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940657.2
CDS	1..3618
Translation	MRGRSNSGVRLDGYARLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940600.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940600.2

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGGC TGCCCTGGAG GCCCTAGATG AGCTGGATCT CTTTGGAGCA 
AAGGGAGGCC CACAGTCTGT GATTCGGGTG CTGTCAGATG AGGTGCAGCA CTGCCAGTCC 
ATCCTCCACT CGATGCTGCC GAGGGCCTCC TCGTCCAAGG AGGTGGATGC CAGTGTGCTC 
TCTGTCATCT CCTACCCAGC CTTTGCTGTG GAGGACAGCG AGCTGGTGGA AATTACCAAG 
CAAGAGATCA TCACAAAGCT GCAGGGGCGC TATGGCTGCT GCCGCTTCCT CCGTGACGGA 
TACAGGACCC CCAGAGAGGA TCCCAACCGC TCCTACTATG AACCTGCAGA GCTGAAGCTC 
TTTGAGAACA TCGAGTGTGA GTGGCCTTTG TTCTGGACAT ACCTGATCAT TGATGGGATT 
TTCAATGGAA ACATGGAGCA GGTGCAAGAG TACCGGGAGG CCCTTGAGGG GATTCTCATC 
AAGGGGAAGA ACGGGGTGCG CCTGGTGCCT GAGCTGTACA GTGTCCCACC AGACAAGGTG 
GATGAGGAAT ACAACAACCC CCACACTGTG GACAGGATAC CCATGGGCAA GCTGCCACTC 
ATGTGGGGCC AGTCCCTCTA CATCCTGGGC TGCCTGATGG CTGAGGGATT CCTGGCTCCT 
GGTGAAATCG ATCCCCTCAA CCGCCGCTTT GCGACTGTCC CCAAGCCCGA CGTGGTGGTG 
CAAGTGTGCA TCCTAGCGGA GACAGAGGGG ATCAAGGCTA TTCTGAAGAA GGAGGGCATC 
CATGTGGAGA CTGTGGCCGA TGTTTACCCC ATCAGAGTGC AGCCTGCTCG CATCCTCAGC 
CACATCTACG CTCGCCTGGG TCGCAACAAG CAGATGAGCT TGACAGGACG GCCCTACCGG 
CACATGGGTG TGATTGGGAC CTCCAAGCTC TACAACATTA GGAAGAACAT CTTTGCCTTC 
ACCCCACAGT TCATAGACCA GCAGCAGTTC TACCTGGCAC TCGACAGCAA GATGATTGTG 
GAGATGCTGA GGACAGACCT CTCATACCTC TGCAGCCGCT GGAGGATGAC TGGACGGCCC 
ACCATCACCT TCCCTGTCTC TCAGACCATG CTCGATGATA CGGGCAGCAG CATACACCCC 
ACAGTGCTGG CAACACTACG GAAGCTGCAG GACGGGTATT TCGGTGGTGC AAGGATCCAG 
ACCGGTAAGT TGTCGGAGTT CCTGACAACG TCGTGCCGAA CGCACCTCAG CTTCATGGAC 
CCTGGGCCGG AGGCGGACAC ATACGATGAG GTTGCACAGT ACCTAGACCA CCTGCTGCAG 
CGCACGGTTC CCCAGTCAAA CCTCCCTCCC ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC 
CGTGCTGCCG TCCACACCAC CCGTGACCTC ATGTCCCTGG CGTCCAAGGC CAAGGACCTT 
CAGGTCCAGA ATGTTGGGAT GTATGTCCCT AGCAAGATCT TCCAGGCATC CCAGCAGTCA 
GTAAAGTTGT TGAGCTCACC CCACCAGCAT GACCAGGAAG TCAAGAGCCA CGCACTCCAT 
GCGGAGATGA ACCTGCCCCG CGATGAGGCT GGCAATGTGG ACTGCAAGGC ACTGGTGGAC 
CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG CAAGCAGACA TCCTCTACAT GCTCCACATC 
CTCAAGGGGC CCGAGTGGCA CACGGGGCTT GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG 
CTCCTCACTG AGCTGTACGT GCGGGTGGGG GCCACGCGCC AGTGGGCTCT GATCCGCTAC 
ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA GCTTTGGATG AGGCCTGCAC GGCCCTGTTG 
TCTCACCAGA AGCACTTGAC AGTGGGGCTG CCCCCAGAGC CACGTGAGAA GACCATCTCT 
GCCCCAATCC CCTACGAGGA GCTCGTTCGC CTCATTGATG AAGCCAGTGA GAAGAACCTC 
AGCGTGTCCA TCCTCACCCA GGAGATCATG GTGTACTTGG CCATGTACAT CCGCACCCAG 
CCAGCGCTCT TCACCGATAT GTTTCGAATC CGCATTGGGC TCATCATCCA GGTGATGGCA 
ACAGAGCTGG CGCACTCCCT GCACTGCTCA GCTGGAGAAG CCACTGAGAA CCTGATGAAT 
CTCAGCCCAT CCAACGTGAA GAACCTCCTC TACCACATCC TCTCCGGCAA GGAATTTGGA 
GTGGAGAAGA GTGTGCGATC GGTGGACTCA TTGGTCACCA CTGCTATCTC CATCTACGAT 
GTGGAGGCTG CTGGAGCCAC CAAGACTGAG CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG 
ATCAAACAGC AACTGGATAA ACGTAGGCAG TCTCTGACCG GGAGTAAGCC TGGGGACGGT 
GACCTGCTGG CCTCTCTGTC TGCTGGGCAA TCGGAGCTGC TGGACAAAGG CTCCTTTTCA 
AGCTTAGTGA AAGACCAGAG CAGTAAGGAC AGCCGCCAGG GCCAGTGGAA ACGGAGACGG 
CGGCTGGATG GGGCCCTCAA CCGTGTCCCC TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC 
CTGCAGAAGT GCCACGGTCT TTCCGTGGAG GGCTTTGTTC TCCCCTCTTC AACAACCAGA 
GAGATGACCC CAGGGGAAAT GAAGTTTGCT GTGCACGTGG AGTCGGTCCT CAACCGTGTG 
CCCCAGCCAG AGTACCGGCA GCTGCTGGTA GAGACGATCT TGGTGCTCAC CATGCTGGTG 
GATGTGGAGG TGCACACCAT GGGTGGCATC ATACCTGTGG AAAAGATCCT GCACGCAGCC 
AATGACCTCT TCTATGAGGA GCAGAAGGCC CTGGGTGCTG ATGACCAGAT GCTGGAGAAG 
GATCCCTCGA CGGGCATTTG CGACCTGCTG TACGACAGCG CACCGAGTGG CCGCTTCGGC 
ACCATGACCT ACCTGTCCAA GTCAGTGGCC CTGCACCTGT ACGACCTCCT GCCCGGAGAT 
GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940601.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940658.2 Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

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CloneID	OGa34668
Clone ID Related Accession (Same CDS sequence)	XM_004940601.2
Accession Version	XM_004940601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGTC CATCCTCCAC TCGATGCTGC CGAGGGCCTC CTCGTCCAAG 
GAGGTGGATG CCAGTGTGCT CTCTGTCATC TCCTACCCAG CCTTTGCTGT GGAGGACAGC 
GAGCTGGTGG AAATTACCAA GCAAGAGATC ATCACAAAGC TGCAGGGGCG CTATGGCTGC 
TGCCGCTTCC TCCGTGACGG ATACAGGACC CCCAGAGAGG ATCCCAACCG CTCCTACTAT 
GAACCTGCAG AGCTGAAGCT CTTTGAGAAC ATCGAGTGTG AGTGGCCTTT GTTCTGGACA 
TACCTGATCA TTGATGGGAT TTTCAATGGA AACATGGAGC AGGTGCAAGA GTACCGGGAG 
GCCCTTGAGG GGATTCTCAT CAAGGGGAAG AACGGGGTGC GCCTGGTGCC TGAGCTGTAC 
AGTGTCCCAC CAGACAAGGT GGATGAGGAA TACAACAACC CCCACACTGT GGACAGGATA 
CCCATGGGCA AGCTGCCACT CATGTGGGGC CAGTCCCTCT ACATCCTGGG CTGCCTGATG 
GCTGAGGGAT TCCTGGCTCC TGGTGAAATC GATCCCCTCA ACCGCCGCTT TGCGACTGTC 
CCCAAGCCCG ACGTGGTGGT GCAAGTGTGC ATCCTAGCGG AGACAGAGGG GATCAAGGCT 
ATTCTGAAGA AGGAGGGCAT CCATGTGGAG ACTGTGGCCG ATGTTTACCC CATCAGAGTG 
CAGCCTGCTC GCATCCTCAG CCACATCTAC GCTCGCCTGG GTCGCAACAA GCAGATGAGC 
TTGACAGGAC GGCCCTACCG GCACATGGGT GTGATTGGGA CCTCCAAGCT CTACAACATT 
AGGAAGAACA TCTTTGCCTT CACCCCACAG TTCATAGACC AGCAGCAGTT CTACCTGGCA 
CTCGACAGCA AGATGATTGT GGAGATGCTG AGGACAGACC TCTCATACCT CTGCAGCCGC 
TGGAGGATGA CTGGACGGCC CACCATCACC TTCCCTGTCT CTCAGACCAT GCTCGATGAT 
ACGGGCAGCA GCATACACCC CACAGTGCTG GCAACACTAC GGAAGCTGCA GGACGGGTAT 
TTCGGTGGTG CAAGGATCCA GACCGGTAAG TTGTCGGAGT TCCTGACAAC GTCGTGCCGA 
ACGCACCTCA GCTTCATGGA CCCTGGGCCG GAGGGTAAGG TCTTTGCCAA CGGTTACAAG 
CTCGGCCTTG ACAGCTTTGA GGAGCTAGAC CCTGAGGAGT GGCTGCATGG CTTGCAGTTT 
GCAGAGGATG CGGACACATA CGATGAGGTT GCACAGTACC TAGACCACCT GCTGCAGCGC 
ACGGTTCCCC AGTCAAACCT CCCTCCCACT GCCCAGCAAG GAGGGCTGAA GCGCTTCCGT 
GCTGCCGTCC ACACCACCCG TGACCTCATG TCCCTGGCGT CCAAGGCCAA GGACCTTCAG 
GTCCAGAATG TTGGGATGTA TGTCCCTAGC AAGATCTTCC AGGCATCCCA GCAGTCAGTA 
AAGTTGTTGA GCTCACCCCA CCAGCATGAC CAGGAAGTCA AGAGCCACGC ACTCCATGCG 
GAGATGAACC TGCCCCGCGA TGAGGCTGGC AATGTGGACT GCAAGGCACT GGTGGACCAG 
CTGCGCATCT GCCCCACGCT GCAGGAGCAA GCAGACATCC TCTACATGCT CCACATCCTC 
AAGGGGCCCG AGTGGCACAC GGGGCTTGAG AGTGAGCCTG GCCCCACTGT GAAGGAGCTC 
CTCACTGAGC TGTACGTGCG GGTGGGGGCC ACGCGCCAGT GGGCTCTGAT CCGCTACATT 
TCTGGTATCC TCAAGAAAAA GGTGGAAGCT TTGGATGAGG CCTGCACGGC CCTGTTGTCT 
CACCAGAAGC ACTTGACAGT GGGGCTGCCC CCAGAGCCAC GTGAGAAGAC CATCTCTGCC 
CCAATCCCCT ACGAGGAGCT CGTTCGCCTC ATTGATGAAG CCAGTGAGAA GAACCTCAGC 
GTGTCCATCC TCACCCAGGA GATCATGGTG TACTTGGCCA TGTACATCCG CACCCAGCCA 
GCGCTCTTCA CCGATATGTT TCGAATCCGC ATTGGGCTCA TCATCCAGGT GATGGCAACA 
GAGCTGGCGC ACTCCCTGCA CTGCTCAGCT GGAGAAGCCA CTGAGAACCT GATGAATCTC 
AGCCCATCCA ACGTGAAGAA CCTCCTCTAC CACATCCTCT CCGGCAAGGA ATTTGGAGTG 
GAGAAGAGTG TGCGATCGGT GGACTCATTG GTCACCACTG CTATCTCCAT CTACGATGTG 
GAGGCTGCTG GAGCCACCAA GACTGAGCGT GCTGGCATCG TCAGGCTGAA AAGTGAGATC 
AAACAGCAAC TGGATAAACG TAGGCAGTCT CTGACCGGGA GTAAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940658.2
CDS	110..3724
Translation	MRGRSNSGVRLDGYARLVQQTILRHQDAVTGLLPASADHRDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLQCMMRQVDKVEAFKYSQSTRDCLHAKYNTHTCATVVGDHEWGHLQLDATSLYLLMLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940601.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940601.2

ORF Insert Sequence:

ATGCGCGGCC GCAGCAACTC GGGCGTGCGG CTGGACGGCT ACGCCCGCCT GGTGCAGCAG 
ACCATCCTCC GGCACCAGGA CGCCGTGACG GGGCTGCTGC CCGCCAGCGC CGACCACCGC 
GATGCGTGGG TCCGCGACAA CGTCTACAGC ATCCTGGCCG TCTGGGGCCT GGGCCTGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCGAAGG CCTACGAGCT GGAGCAGAGC 
GTGGTGAAGC TGATGAGGGG GCTGCTCCAG TGCATGATGA GACAGGTGGA CAAGGTGGAG 
GCCTTCAAGT ACAGCCAGAG CACCCGGGAC TGCCTGCATG CCAAATACAA CACCCACACC 
TGTGCCACTG TGGTGGGAGA CCACGAGTGG GGGCACCTGC AGTTGGATGC CACCTCCCTC 
TACCTGCTCA TGCTGGCGCA GATGACAGCC TCAGGCCTCC ACATAATTCA CAGCCTGGAT 
GAAGTCAACT TCATCCAGAA CCTCGTCTTC TACATCGAAG CGGCCTATAA GACAGCGGAC 
TTTGGGATAT GGGAACGTGG GGACAAGACG AACCGGGGGA TCACTGAGTT GAATGCCAGC 
TCTGTCGGCA TGGCCAAGTC CATCCTCCAC TCGATGCTGC CGAGGGCCTC CTCGTCCAAG 
GAGGTGGATG CCAGTGTGCT CTCTGTCATC TCCTACCCAG CCTTTGCTGT GGAGGACAGC 
GAGCTGGTGG AAATTACCAA GCAAGAGATC ATCACAAAGC TGCAGGGGCG CTATGGCTGC 
TGCCGCTTCC TCCGTGACGG ATACAGGACC CCCAGAGAGG ATCCCAACCG CTCCTACTAT 
GAACCTGCAG AGCTGAAGCT CTTTGAGAAC ATCGAGTGTG AGTGGCCTTT GTTCTGGACA 
TACCTGATCA TTGATGGGAT TTTCAATGGA AACATGGAGC AGGTGCAAGA GTACCGGGAG 
GCCCTTGAGG GGATTCTCAT CAAGGGGAAG AACGGGGTGC GCCTGGTGCC TGAGCTGTAC 
AGTGTCCCAC CAGACAAGGT GGATGAGGAA TACAACAACC CCCACACTGT GGACAGGATA 
CCCATGGGCA AGCTGCCACT CATGTGGGGC CAGTCCCTCT ACATCCTGGG CTGCCTGATG 
GCTGAGGGAT TCCTGGCTCC TGGTGAAATC GATCCCCTCA ACCGCCGCTT TGCGACTGTC 
CCCAAGCCCG ACGTGGTGGT GCAAGTGTGC ATCCTAGCGG AGACAGAGGG GATCAAGGCT 
ATTCTGAAGA AGGAGGGCAT CCATGTGGAG ACTGTGGCCG ATGTTTACCC CATCAGAGTG 
CAGCCTGCTC GCATCCTCAG CCACATCTAC GCTCGCCTGG GTCGCAACAA GCAGATGAGC 
TTGACAGGAC GGCCCTACCG GCACATGGGT GTGATTGGGA CCTCCAAGCT CTACAACATT 
AGGAAGAACA TCTTTGCCTT CACCCCACAG TTCATAGACC AGCAGCAGTT CTACCTGGCA 
CTCGACAGCA AGATGATTGT GGAGATGCTG AGGACAGACC TCTCATACCT CTGCAGCCGC 
TGGAGGATGA CTGGACGGCC CACCATCACC TTCCCTGTCT CTCAGACCAT GCTCGATGAT 
ACGGGCAGCA GCATACACCC CACAGTGCTG GCAACACTAC GGAAGCTGCA GGACGGGTAT 
TTCGGTGGTG CAAGGATCCA GACCGGTAAG TTGTCGGAGT TCCTGACAAC GTCGTGCCGA 
ACGCACCTCA GCTTCATGGA CCCTGGGCCG GAGGGTAAGG TCTTTGCCAA CGGTTACAAG 
CTCGGCCTTG ACAGCTTTGA GGAGCTAGAC CCTGAGGAGT GGCTGCATGG CTTGCAGTTT 
GCAGAGGATG CGGACACATA CGATGAGGTT GCACAGTACC TAGACCACCT GCTGCAGCGC 
ACGGTTCCCC AGTCAAACCT CCCTCCCACT GCCCAGCAAG GAGGGCTGAA GCGCTTCCGT 
GCTGCCGTCC ACACCACCCG TGACCTCATG TCCCTGGCGT CCAAGGCCAA GGACCTTCAG 
GTCCAGAATG TTGGGATGTA TGTCCCTAGC AAGATCTTCC AGGCATCCCA GCAGTCAGTA 
AAGTTGTTGA GCTCACCCCA CCAGCATGAC CAGGAAGTCA AGAGCCACGC ACTCCATGCG 
GAGATGAACC TGCCCCGCGA TGAGGCTGGC AATGTGGACT GCAAGGCACT GGTGGACCAG 
CTGCGCATCT GCCCCACGCT GCAGGAGCAA GCAGACATCC TCTACATGCT CCACATCCTC 
AAGGGGCCCG AGTGGCACAC GGGGCTTGAG AGTGAGCCTG GCCCCACTGT GAAGGAGCTC 
CTCACTGAGC TGTACGTGCG GGTGGGGGCC ACGCGCCAGT GGGCTCTGAT CCGCTACATT 
TCTGGTATCC TCAAGAAAAA GGTGGAAGCT TTGGATGAGG CCTGCACGGC CCTGTTGTCT 
CACCAGAAGC ACTTGACAGT GGGGCTGCCC CCAGAGCCAC GTGAGAAGAC CATCTCTGCC 
CCAATCCCCT ACGAGGAGCT CGTTCGCCTC ATTGATGAAG CCAGTGAGAA GAACCTCAGC 
GTGTCCATCC TCACCCAGGA GATCATGGTG TACTTGGCCA TGTACATCCG CACCCAGCCA 
GCGCTCTTCA CCGATATGTT TCGAATCCGC ATTGGGCTCA TCATCCAGGT GATGGCAACA 
GAGCTGGCGC ACTCCCTGCA CTGCTCAGCT GGAGAAGCCA CTGAGAACCT GATGAATCTC 
AGCCCATCCA ACGTGAAGAA CCTCCTCTAC CACATCCTCT CCGGCAAGGA ATTTGGAGTG 
GAGAAGAGTG TGCGATCGGT GGACTCATTG GTCACCACTG CTATCTCCAT CTACGATGTG 
GAGGCTGCTG GAGCCACCAA GACTGAGCGT GCTGGCATCG TCAGGCTGAA AAGTGAGATC 
AAACAGCAAC TGGATAAACG TAGGCAGTCT CTGACCGGGA GTAAGCCTGG GGACGGTGAC 
CTGCTGGCCT CTCTGTCTGC TGGGCAATCG GAGCTGCTGG ACAAAGGCTC CTTTTCAAGC 
TTAGTGAAAG ACCAGAGCAG TAAGGACAGC CGCCAGGGCC AGTGGAAACG GAGACGGCGG 
CTGGATGGGG CCCTCAACCG TGTCCCCTTG GGTTTCTACC AGAAGGTCTG GAAGGTCCTG 
CAGAAGTGCC ACGGTCTTTC CGTGGAGGGC TTTGTTCTCC CCTCTTCAAC AACCAGAGAG 
ATGACCCCAG GGGAAATGAA GTTTGCTGTG CACGTGGAGT CGGTCCTCAA CCGTGTGCCC 
CAGCCAGAGT ACCGGCAGCT GCTGGTAGAG ACGATCTTGG TGCTCACCAT GCTGGTGGAT 
GTGGAGGTGC ACACCATGGG TGGCATCATA CCTGTGGAAA AGATCCTGCA CGCAGCCAAT 
GACCTCTTCT ATGAGGAGCA GAAGGCCCTG GGTGCTGATG ACCAGATGCT GGAGAAGGAT 
CCCTCGACGG GCATTTGCGA CCTGCTGTAC GACAGCGCAC CGAGTGGCCG CTTCGGCACC 
ATGACCTACC TGTCCAAGTC AGTGGCCCTG CACCTGTACG ACCTCCTGCC CGGAGATGGC 
TGTGCCATGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940602.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940659.1 Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa07307
Clone ID Related Accession (Same CDS sequence)	XM_004940602.2 , XM_004940602.3
Accession Version	XM_004940602.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763735.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGA GGACGTGGCC ACCGCAGGGC CTCCACATAA TTCACAGCCT GGATGAAGTC 
AACTTCATCC AGAACCTCGT CTTCTACATC GAAGCGGCCT ATAAGACAGC GGACTTTGGG 
ATATGGGAAC GTGGGGACAA GACGAACCGG GGGATCACTG AGTTGAATGC CAGCTCTGTC 
GGCATGGCCA AGGCTGCCCT GGAGGCCCTA GATGAGCTGG ATCTCTTTGG AGCAAAGGGA 
GGCCCACAGT CTGTGATTCG GGTGCTGTCA GATGAGGTGC AGCACTGCCA GTCCATCCTC 
CACTCGATGC TGCCGAGGGC CTCCTCGTCC AAGGAGGTGG ATGCCAGTGT GCTCTCTGTC 
ATCTCCTACC CAGCCTTTGC TGTGGAGGAC AGCGAGCTGG TGGAAATTAC CAAGCAAGAG 
ATCATCACAA AGCTGCAGGG GCGCTATGGC TGCTGCCGCT TCCTCCGTGA CGGATACAGG 
ACCCCCAGAG AGGATCCCAA CCGCTCCTAC TATGAACCTG CAGAGCTGAA GCTCTTTGAG 
AACATCGAGT GTGAGTGGCC TTTGTTCTGG ACATACCTGA TCATTGATGG GATTTTCAAT 
GGAAACATGG AGCAGGTGCA AGAGTACCGG GAGGCCCTTG AGGGGATTCT CATCAAGGGG 
AAGAACGGGG TGCGCCTGGT GCCTGAGCTG TACAGTGTCC CACCAGACAA GGTGGATGAG 
GAATACAACA ACCCCCACAC TGTGGACAGG ATACCCATGG GCAAGCTGCC ACTCATGTGG 
GGCCAGTCCC TCTACATCCT GGGCTGCCTG ATGGCTGAGG GATTCCTGGC TCCTGGTGAA 
ATCGATCCCC TCAACCGCCG CTTTGCGACT GTCCCCAAGC CCGACGTGGT GGTGCAAGTG 
TGCATCCTAG CGGAGACAGA GGGGATCAAG GCTATTCTGA AGAAGGAGGG CATCCATGTG 
GAGACTGTGG CCGATGTTTA CCCCATCAGA GTGCAGCCTG CTCGCATCCT CAGCCACATC 
TACGCTCGCC TGGGTCGCAA CAAGCAGATG AGCTTGACAG GACGGCCCTA CCGGCACATG 
GGTGTGATTG GGACCTCCAA GCTCTACAAC ATTAGGAAGA ACATCTTTGC CTTCACCCCA 
CAGTTCATAG ACCAGCAGCA GTTCTACCTG GCACTCGACA GCAAGATGAT TGTGGAGATG 
CTGAGGACAG ACCTCTCATA CCTCTGCAGC CGCTGGAGGA TGACTGGACG GCCCACCATC 
ACCTTCCCTG TCTCTCAGAC CATGCTCGAT GATACGGGCA GCAGCATACA CCCCACAGTG 
CTGGCAACAC TACGGAAGCT GCAGGACGGG TATTTCGGTG GTGCAAGGAT CCAGACCGGT 
AAGTTGTCGG AGTTCCTGAC AACGTCGTGC CGAACGCACC TCAGCTTCAT GGACCCTGGG 
CCGGAGGGTA AGGTCTTTGC CAACGGTTAC AAGCTCGGCC TTGACAGCTT TGAGGAGCTA 
GACCCTGAGG AGTGGCTGCA TGGCTTGCAG TTTGCAGAGG ATGCGGACAC ATACGATGAG 
GTTGCACAGT ACCTAGACCA CCTGCTGCAG CGCACGGTTC CCCAGTCAAA CCTCCCTCCC 
ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC CGTGCTGCCG TCCACACCAC CCGTGACCTC 
ATGTCCCTGG CGTCCAAGGC CAAGGACCTT CAGGTCCAGA ATGTTGGGAT GTATGTCCCT 
AGCAAGATCT TCCAGGCATC CCAGCAGTCA GTAAAGTTGT TGAGCTCACC CCACCAGCAT 
GACCAGGAAG TCAAGAGCCA CGCACTCCAT GCGGAGATGA ACCTGCCCCG CGATGAGGCT 
GGCAATGTGG ACTGCAAGGC ACTGGTGGAC CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG 
CAAGCAGACA TCCTCTACAT GCTCCACATC CTCAAGGGGC CCGAGTGGCA CACGGGGCTT 
GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG CTCCTCACTG AGCTGTACGT GCGGGTGGGG 
GCCACGCGCC AGTGGGCTCT GATCCGCTAC ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA 
GCTTTGGATG AGGCCTGCAC GGCCCTGTTG TCTCACCAGA AGCACTTGAC AGTGGGGCTG 
CCCCCAGAGC CACGTGAGAA GACCATCTCT GCCCCAATCC CCTACGAGGA GCTCGTTCGC 
CTCATTGATG AAGCCAGTGA GAAGAACCTC AGCGTGTCCA TCCTCACCCA GGAGATCATG 
GTGTACTTGG CCATGTACAT CCGCACCCAG CCAGCGCTCT TCACCGATAT GTTTCGAATC 
CGCATTGGGC TCATCATCCA GGTGATGGCA ACAGAGCTGG CGCACTCCCT GCACTGCTCA 
GCTGGAGAAG CCACTGAGAA CCTGATGAAT CTCAGCCCAT CCAACGTGAA GAACCTCCTC 
TACCACATCC TCTCCGGCAA GGAATTTGGA GTGGAGAAGA GTGTGCGATC GGTGGACTCA 
TTGGTCACCA CTGCTATCTC CATCTACGAT GTGGAGGCTG CTGGAGCCAC CAAGACTGAG 
CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG ATCAAACAGC AACTGGATAA ACGTAGGCAG 
TCTCTGACCG GGAGTAAGCC TGGGGACGGT GACCTGCTGG CCTCTCTGTC TGCTGGGCAA 
TCGGAGCTGC TGGACAAAGG CTCCTTTTCA AGCTTAGTGA AAGACCAGAG CAGTAAGGAC 
AGCCGCCAGG GCCAGTGGAA ACGGAGACGG CGGCTGGATG GGGCCCTCAA CCGTGTCCCC 
TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC CTGCAGAAGT GCCACGGTCT TTCCGTGGAG 
GGCTTTGTTC TCCCCTCTTC AACAACCAGA GAGATGACCC CAGGGGAAAT GAAGTTTGCT 
GTGCACGTGG AGTCGGTCCT CAACCGTGTG CCCCAGCCAG AGTACCGGCA GCTGCTGGTA 
GAGACGATCT TGGTGCTCAC CATGCTGGTG GATGTGGAGG TGCACACCAT GGGTGGCATC 
ATACCTGTGG AAAAGATCCT GCACGCAGCC AATGACCTCT TCTATGAGGA GCAGAAGGCC 
CTGGGTGCTG ATGACCAGAT GCTGGAGAAG GATCCCTCGA CGGGCATTTG CGACCTGCTG 
TACGACAGCG CACCGAGTGG CCGCTTCGGC ACCATGACCT ACCTGTCCAA GTCAGTGGCC 
CTGCACCTGT ACGACCTCCT GCCCGGAGAT GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940659.1
CDS	45..3332
Translation	MGVRTWPPQGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940602.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940602.2

ORF Insert Sequence:

ATGGGAGTGA GGACGTGGCC ACCGCAGGGC CTCCACATAA TTCACAGCCT GGATGAAGTC 
AACTTCATCC AGAACCTCGT CTTCTACATC GAAGCGGCCT ATAAGACAGC GGACTTTGGG 
ATATGGGAAC GTGGGGACAA GACGAACCGG GGGATCACTG AGTTGAATGC CAGCTCTGTC 
GGCATGGCCA AGGCTGCCCT GGAGGCCCTA GATGAGCTGG ATCTCTTTGG AGCAAAGGGA 
GGCCCACAGT CTGTGATTCG GGTGCTGTCA GATGAGGTGC AGCACTGCCA GTCCATCCTC 
CACTCGATGC TGCCGAGGGC CTCCTCGTCC AAGGAGGTGG ATGCCAGTGT GCTCTCTGTC 
ATCTCCTACC CAGCCTTTGC TGTGGAGGAC AGCGAGCTGG TGGAAATTAC CAAGCAAGAG 
ATCATCACAA AGCTGCAGGG GCGCTATGGC TGCTGCCGCT TCCTCCGTGA CGGATACAGG 
ACCCCCAGAG AGGATCCCAA CCGCTCCTAC TATGAACCTG CAGAGCTGAA GCTCTTTGAG 
AACATCGAGT GTGAGTGGCC TTTGTTCTGG ACATACCTGA TCATTGATGG GATTTTCAAT 
GGAAACATGG AGCAGGTGCA AGAGTACCGG GAGGCCCTTG AGGGGATTCT CATCAAGGGG 
AAGAACGGGG TGCGCCTGGT GCCTGAGCTG TACAGTGTCC CACCAGACAA GGTGGATGAG 
GAATACAACA ACCCCCACAC TGTGGACAGG ATACCCATGG GCAAGCTGCC ACTCATGTGG 
GGCCAGTCCC TCTACATCCT GGGCTGCCTG ATGGCTGAGG GATTCCTGGC TCCTGGTGAA 
ATCGATCCCC TCAACCGCCG CTTTGCGACT GTCCCCAAGC CCGACGTGGT GGTGCAAGTG 
TGCATCCTAG CGGAGACAGA GGGGATCAAG GCTATTCTGA AGAAGGAGGG CATCCATGTG 
GAGACTGTGG CCGATGTTTA CCCCATCAGA GTGCAGCCTG CTCGCATCCT CAGCCACATC 
TACGCTCGCC TGGGTCGCAA CAAGCAGATG AGCTTGACAG GACGGCCCTA CCGGCACATG 
GGTGTGATTG GGACCTCCAA GCTCTACAAC ATTAGGAAGA ACATCTTTGC CTTCACCCCA 
CAGTTCATAG ACCAGCAGCA GTTCTACCTG GCACTCGACA GCAAGATGAT TGTGGAGATG 
CTGAGGACAG ACCTCTCATA CCTCTGCAGC CGCTGGAGGA TGACTGGACG GCCCACCATC 
ACCTTCCCTG TCTCTCAGAC CATGCTCGAT GATACGGGCA GCAGCATACA CCCCACAGTG 
CTGGCAACAC TACGGAAGCT GCAGGACGGG TATTTCGGTG GTGCAAGGAT CCAGACCGGT 
AAGTTGTCGG AGTTCCTGAC AACGTCGTGC CGAACGCACC TCAGCTTCAT GGACCCTGGG 
CCGGAGGGTA AGGTCTTTGC CAACGGTTAC AAGCTCGGCC TTGACAGCTT TGAGGAGCTA 
GACCCTGAGG AGTGGCTGCA TGGCTTGCAG TTTGCAGAGG ATGCGGACAC ATACGATGAG 
GTTGCACAGT ACCTAGACCA CCTGCTGCAG CGCACGGTTC CCCAGTCAAA CCTCCCTCCC 
ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC CGTGCTGCCG TCCACACCAC CCGTGACCTC 
ATGTCCCTGG CGTCCAAGGC CAAGGACCTT CAGGTCCAGA ATGTTGGGAT GTATGTCCCT 
AGCAAGATCT TCCAGGCATC CCAGCAGTCA GTAAAGTTGT TGAGCTCACC CCACCAGCAT 
GACCAGGAAG TCAAGAGCCA CGCACTCCAT GCGGAGATGA ACCTGCCCCG CGATGAGGCT 
GGCAATGTGG ACTGCAAGGC ACTGGTGGAC CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG 
CAAGCAGACA TCCTCTACAT GCTCCACATC CTCAAGGGGC CCGAGTGGCA CACGGGGCTT 
GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG CTCCTCACTG AGCTGTACGT GCGGGTGGGG 
GCCACGCGCC AGTGGGCTCT GATCCGCTAC ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA 
GCTTTGGATG AGGCCTGCAC GGCCCTGTTG TCTCACCAGA AGCACTTGAC AGTGGGGCTG 
CCCCCAGAGC CACGTGAGAA GACCATCTCT GCCCCAATCC CCTACGAGGA GCTCGTTCGC 
CTCATTGATG AAGCCAGTGA GAAGAACCTC AGCGTGTCCA TCCTCACCCA GGAGATCATG 
GTGTACTTGG CCATGTACAT CCGCACCCAG CCAGCGCTCT TCACCGATAT GTTTCGAATC 
CGCATTGGGC TCATCATCCA GGTGATGGCA ACAGAGCTGG CGCACTCCCT GCACTGCTCA 
GCTGGAGAAG CCACTGAGAA CCTGATGAAT CTCAGCCCAT CCAACGTGAA GAACCTCCTC 
TACCACATCC TCTCCGGCAA GGAATTTGGA GTGGAGAAGA GTGTGCGATC GGTGGACTCA 
TTGGTCACCA CTGCTATCTC CATCTACGAT GTGGAGGCTG CTGGAGCCAC CAAGACTGAG 
CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG ATCAAACAGC AACTGGATAA ACGTAGGCAG 
TCTCTGACCG GGAGTAAGCC TGGGGACGGT GACCTGCTGG CCTCTCTGTC TGCTGGGCAA 
TCGGAGCTGC TGGACAAAGG CTCCTTTTCA AGCTTAGTGA AAGACCAGAG CAGTAAGGAC 
AGCCGCCAGG GCCAGTGGAA ACGGAGACGG CGGCTGGATG GGGCCCTCAA CCGTGTCCCC 
TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC CTGCAGAAGT GCCACGGTCT TTCCGTGGAG 
GGCTTTGTTC TCCCCTCTTC AACAACCAGA GAGATGACCC CAGGGGAAAT GAAGTTTGCT 
GTGCACGTGG AGTCGGTCCT CAACCGTGTG CCCCAGCCAG AGTACCGGCA GCTGCTGGTA 
GAGACGATCT TGGTGCTCAC CATGCTGGTG GATGTGGAGG TGCACACCAT GGGTGGCATC 
ATACCTGTGG AAAAGATCCT GCACGCAGCC AATGACCTCT TCTATGAGGA GCAGAAGGCC 
CTGGGTGCTG ATGACCAGAT GCTGGAGAAG GATCCCTCGA CGGGCATTTG CGACCTGCTG 
TACGACAGCG CACCGAGTGG CCGCTTCGGC ACCATGACCT ACCTGTCCAA GTCAGTGGCC 
CTGCACCTGT ACGACCTCCT GCCCGGAGAT GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA1 ( XM_004940602.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940659.1 Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.

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CloneID	OGa07307
Clone ID Related Accession (Same CDS sequence)	XM_004940602.2 , XM_004940602.3
Accession Version	XM_004940602.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940602.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTGA GGACGTGGCC ACCGCAGGGC CTCCACATAA TTCACAGCCT GGATGAAGTC 
AACTTCATCC AGAACCTCGT CTTCTACATC GAAGCGGCCT ATAAGACAGC GGACTTTGGG 
ATATGGGAAC GTGGGGACAA GACGAACCGG GGGATCACTG AGTTGAATGC CAGCTCTGTC 
GGCATGGCCA AGGCTGCCCT GGAGGCCCTA GATGAGCTGG ATCTCTTTGG AGCAAAGGGA 
GGCCCACAGT CTGTGATTCG GGTGCTGTCA GATGAGGTGC AGCACTGCCA GTCCATCCTC 
CACTCGATGC TGCCGAGGGC CTCCTCGTCC AAGGAGGTGG ATGCCAGTGT GCTCTCTGTC 
ATCTCCTACC CAGCCTTTGC TGTGGAGGAC AGCGAGCTGG TGGAAATTAC CAAGCAAGAG 
ATCATCACAA AGCTGCAGGG GCGCTATGGC TGCTGCCGCT TCCTCCGTGA CGGATACAGG 
ACCCCCAGAG AGGATCCCAA CCGCTCCTAC TATGAACCTG CAGAGCTGAA GCTCTTTGAG 
AACATCGAGT GTGAGTGGCC TTTGTTCTGG ACATACCTGA TCATTGATGG GATTTTCAAT 
GGAAACATGG AGCAGGTGCA AGAGTACCGG GAGGCCCTTG AGGGGATTCT CATCAAGGGG 
AAGAACGGGG TGCGCCTGGT GCCTGAGCTG TACAGTGTCC CACCAGACAA GGTGGATGAG 
GAATACAACA ACCCCCACAC TGTGGACAGG ATACCCATGG GCAAGCTGCC ACTCATGTGG 
GGCCAGTCCC TCTACATCCT GGGCTGCCTG ATGGCTGAGG GATTCCTGGC TCCTGGTGAA 
ATCGATCCCC TCAACCGCCG CTTTGCGACT GTCCCCAAGC CCGACGTGGT GGTGCAAGTG 
TGCATCCTAG CGGAGACAGA GGGGATCAAG GCTATTCTGA AGAAGGAGGG CATCCATGTG 
GAGACTGTGG CCGATGTTTA CCCCATCAGA GTGCAGCCTG CTCGCATCCT CAGCCACATC 
TACGCTCGCC TGGGTCGCAA CAAGCAGATG AGCTTGACAG GACGGCCCTA CCGGCACATG 
GGTGTGATTG GGACCTCCAA GCTCTACAAC ATTAGGAAGA ACATCTTTGC CTTCACCCCA 
CAGTTCATAG ACCAGCAGCA GTTCTACCTG GCACTCGACA GCAAGATGAT TGTGGAGATG 
CTGAGGACAG ACCTCTCATA CCTCTGCAGC CGCTGGAGGA TGACTGGACG GCCCACCATC 
ACCTTCCCTG TCTCTCAGAC CATGCTCGAT GATACGGGCA GCAGCATACA CCCCACAGTG 
CTGGCAACAC TACGGAAGCT GCAGGACGGG TATTTCGGTG GTGCAAGGAT CCAGACCGGT 
AAGTTGTCGG AGTTCCTGAC AACGTCGTGC CGAACGCACC TCAGCTTCAT GGACCCTGGG 
CCGGAGGGTA AGGTCTTTGC CAACGGTTAC AAGCTCGGCC TTGACAGCTT TGAGGAGCTA 
GACCCTGAGG AGTGGCTGCA TGGCTTGCAG TTTGCAGAGG ATGCGGACAC ATACGATGAG 
GTTGCACAGT ACCTAGACCA CCTGCTGCAG CGCACGGTTC CCCAGTCAAA CCTCCCTCCC 
ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC CGTGCTGCCG TCCACACCAC CCGTGACCTC 
ATGTCCCTGG CGTCCAAGGC CAAGGACCTT CAGGTCCAGA ATGTTGGGAT GTATGTCCCT 
AGCAAGATCT TCCAGGCATC CCAGCAGTCA GTAAAGTTGT TGAGCTCACC CCACCAGCAT 
GACCAGGAAG TCAAGAGCCA CGCACTCCAT GCGGAGATGA ACCTGCCCCG CGATGAGGCT 
GGCAATGTGG ACTGCAAGGC ACTGGTGGAC CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG 
CAAGCAGACA TCCTCTACAT GCTCCACATC CTCAAGGGGC CCGAGTGGCA CACGGGGCTT 
GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG CTCCTCACTG AGCTGTACGT GCGGGTGGGG 
GCCACGCGCC AGTGGGCTCT GATCCGCTAC ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA 
GCTTTGGATG AGGCCTGCAC GGCCCTGTTG TCTCACCAGA AGCACTTGAC AGTGGGGCTG 
CCCCCAGAGC CACGTGAGAA GACCATCTCT GCCCCAATCC CCTACGAGGA GCTCGTTCGC 
CTCATTGATG AAGCCAGTGA GAAGAACCTC AGCGTGTCCA TCCTCACCCA GGAGATCATG 
GTGTACTTGG CCATGTACAT CCGCACCCAG CCAGCGCTCT TCACCGATAT GTTTCGAATC 
CGCATTGGGC TCATCATCCA GGTGATGGCA ACAGAGCTGG CGCACTCCCT GCACTGCTCA 
GCTGGAGAAG CCACTGAGAA CCTGATGAAT CTCAGCCCAT CCAACGTGAA GAACCTCCTC 
TACCACATCC TCTCCGGCAA GGAATTTGGA GTGGAGAAGA GTGTGCGATC GGTGGACTCA 
TTGGTCACCA CTGCTATCTC CATCTACGAT GTGGAGGCTG CTGGAGCCAC CAAGACTGAG 
CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG ATCAAACAGC AACTGGATAA ACGTAGGCAG 
TCTCTGACCG GGAGTAAGCC TGGGGACGGT GACCTGCTGG CCTCTCTGTC TGCTGGGCAA 
TCGGAGCTGC TGGACAAAGG CTCCTTTTCA AGCTTAGTGA AAGACCAGAG CAGTAAGGAC 
AGCCGCCAGG GCCAGTGGAA ACGGAGACGG CGGCTGGATG GGGCCCTCAA CCGTGTCCCC 
TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC CTGCAGAAGT GCCACGGTCT TTCCGTGGAG 
GGCTTTGTTC TCCCCTCTTC AACAACCAGA GAGATGACCC CAGGGGAAAT GAAGTTTGCT 
GTGCACGTGG AGTCGGTCCT CAACCGTGTG CCCCAGCCAG AGTACCGGCA GCTGCTGGTA 
GAGACGATCT TGGTGCTCAC CATGCTGGTG GATGTGGAGG TGCACACCAT GGGTGGCATC 
ATACCTGTGG AAAAGATCCT GCACGCAGCC AATGACCTCT TCTATGAGGA GCAGAAGGCC 
CTGGGTGCTG ATGACCAGAT GCTGGAGAAG GATCCCTCGA CGGGCATTTG CGACCTGCTG 
TACGACAGCG CACCGAGTGG CCGCTTCGGC ACCATGACCT ACCTGTCCAA GTCAGTGGCC 
CTGCACCTGT ACGACCTCCT GCCCGGAGAT GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940659.1
CDS	84..3371
Translation	MGVRTWPPQGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNRGITELNASSVGMAKAALEALDELDLFGAKGGPQSVIRVLSDEVQHCQSILHSMLPRASSSKEVDASVLSVISYPAFAVEDSELVEITKQEIITKLQGRYGCCRFLRDGYRTPREDPNRSYYEPAELKLFENIECEWPLFWTYLIIDGIFNGNMEQVQEYREALEGILIKGKNGVRLVPELYSVPPDKVDEEYNNPHTVDRIPMGKLPLMWGQSLYILGCLMAEGFLAPGEIDPLNRRFATVPKPDVVVQVCILAETEGIKAILKKEGIHVETVADVYPIRVQPARILSHIYARLGRNKQMSLTGRPYRHMGVIGTSKLYNIRKNIFAFTPQFIDQQQFYLALDSKMIVEMLRTDLSYLCSRWRMTGRPTITFPVSQTMLDDTGSSIHPTVLATLRKLQDGYFGGARIQTGKLSEFLTTSCRTHLSFMDPGPEGKVFANGYKLGLDSFEELDPEEWLHGLQFAEDADTYDEVAQYLDHLLQRTVPQSNLPPTAQQGGLKRFRAAVHTTRDLMSLASKAKDLQVQNVGMYVPSKIFQASQQSVKLLSSPHQHDQEVKSHALHAEMNLPRDEAGNVDCKALVDQLRICPTLQEQADILYMLHILKGPEWHTGLESEPGPTVKELLTELYVRVGATRQWALIRYISGILKKKVEALDEACTALLSHQKHLTVGLPPEPREKTISAPIPYEELVRLIDEASEKNLSVSILTQEIMVYLAMYIRTQPALFTDMFRIRIGLIIQVMATELAHSLHCSAGEATENLMNLSPSNVKNLLYHILSGKEFGVEKSVRSVDSLVTTAISIYDVEAAGATKTERAGIVRLKSEIKQQLDKRRQSLTGSKPGDGDLLASLSAGQSELLDKGSFSSLVKDQSSKDSRQGQWKRRRRLDGALNRVPLGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEMKFAVHVESVLNRVPQPEYRQLLVETILVLTMLVDVEVHTMGGIIPVEKILHAANDLFYEEQKALGADDQMLEKDPSTGICDLLYDSAPSGRFGTMTYLSKSVALHLYDLLPGDGCAMQ

Target ORF information:

RefSeq Version	XM_004940602.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940602.3

ORF Insert Sequence:

ATGGGAGTGA GGACGTGGCC ACCGCAGGGC CTCCACATAA TTCACAGCCT GGATGAAGTC 
AACTTCATCC AGAACCTCGT CTTCTACATC GAAGCGGCCT ATAAGACAGC GGACTTTGGG 
ATATGGGAAC GTGGGGACAA GACGAACCGG GGGATCACTG AGTTGAATGC CAGCTCTGTC 
GGCATGGCCA AGGCTGCCCT GGAGGCCCTA GATGAGCTGG ATCTCTTTGG AGCAAAGGGA 
GGCCCACAGT CTGTGATTCG GGTGCTGTCA GATGAGGTGC AGCACTGCCA GTCCATCCTC 
CACTCGATGC TGCCGAGGGC CTCCTCGTCC AAGGAGGTGG ATGCCAGTGT GCTCTCTGTC 
ATCTCCTACC CAGCCTTTGC TGTGGAGGAC AGCGAGCTGG TGGAAATTAC CAAGCAAGAG 
ATCATCACAA AGCTGCAGGG GCGCTATGGC TGCTGCCGCT TCCTCCGTGA CGGATACAGG 
ACCCCCAGAG AGGATCCCAA CCGCTCCTAC TATGAACCTG CAGAGCTGAA GCTCTTTGAG 
AACATCGAGT GTGAGTGGCC TTTGTTCTGG ACATACCTGA TCATTGATGG GATTTTCAAT 
GGAAACATGG AGCAGGTGCA AGAGTACCGG GAGGCCCTTG AGGGGATTCT CATCAAGGGG 
AAGAACGGGG TGCGCCTGGT GCCTGAGCTG TACAGTGTCC CACCAGACAA GGTGGATGAG 
GAATACAACA ACCCCCACAC TGTGGACAGG ATACCCATGG GCAAGCTGCC ACTCATGTGG 
GGCCAGTCCC TCTACATCCT GGGCTGCCTG ATGGCTGAGG GATTCCTGGC TCCTGGTGAA 
ATCGATCCCC TCAACCGCCG CTTTGCGACT GTCCCCAAGC CCGACGTGGT GGTGCAAGTG 
TGCATCCTAG CGGAGACAGA GGGGATCAAG GCTATTCTGA AGAAGGAGGG CATCCATGTG 
GAGACTGTGG CCGATGTTTA CCCCATCAGA GTGCAGCCTG CTCGCATCCT CAGCCACATC 
TACGCTCGCC TGGGTCGCAA CAAGCAGATG AGCTTGACAG GACGGCCCTA CCGGCACATG 
GGTGTGATTG GGACCTCCAA GCTCTACAAC ATTAGGAAGA ACATCTTTGC CTTCACCCCA 
CAGTTCATAG ACCAGCAGCA GTTCTACCTG GCACTCGACA GCAAGATGAT TGTGGAGATG 
CTGAGGACAG ACCTCTCATA CCTCTGCAGC CGCTGGAGGA TGACTGGACG GCCCACCATC 
ACCTTCCCTG TCTCTCAGAC CATGCTCGAT GATACGGGCA GCAGCATACA CCCCACAGTG 
CTGGCAACAC TACGGAAGCT GCAGGACGGG TATTTCGGTG GTGCAAGGAT CCAGACCGGT 
AAGTTGTCGG AGTTCCTGAC AACGTCGTGC CGAACGCACC TCAGCTTCAT GGACCCTGGG 
CCGGAGGGTA AGGTCTTTGC CAACGGTTAC AAGCTCGGCC TTGACAGCTT TGAGGAGCTA 
GACCCTGAGG AGTGGCTGCA TGGCTTGCAG TTTGCAGAGG ATGCGGACAC ATACGATGAG 
GTTGCACAGT ACCTAGACCA CCTGCTGCAG CGCACGGTTC CCCAGTCAAA CCTCCCTCCC 
ACTGCCCAGC AAGGAGGGCT GAAGCGCTTC CGTGCTGCCG TCCACACCAC CCGTGACCTC 
ATGTCCCTGG CGTCCAAGGC CAAGGACCTT CAGGTCCAGA ATGTTGGGAT GTATGTCCCT 
AGCAAGATCT TCCAGGCATC CCAGCAGTCA GTAAAGTTGT TGAGCTCACC CCACCAGCAT 
GACCAGGAAG TCAAGAGCCA CGCACTCCAT GCGGAGATGA ACCTGCCCCG CGATGAGGCT 
GGCAATGTGG ACTGCAAGGC ACTGGTGGAC CAGCTGCGCA TCTGCCCCAC GCTGCAGGAG 
CAAGCAGACA TCCTCTACAT GCTCCACATC CTCAAGGGGC CCGAGTGGCA CACGGGGCTT 
GAGAGTGAGC CTGGCCCCAC TGTGAAGGAG CTCCTCACTG AGCTGTACGT GCGGGTGGGG 
GCCACGCGCC AGTGGGCTCT GATCCGCTAC ATTTCTGGTA TCCTCAAGAA AAAGGTGGAA 
GCTTTGGATG AGGCCTGCAC GGCCCTGTTG TCTCACCAGA AGCACTTGAC AGTGGGGCTG 
CCCCCAGAGC CACGTGAGAA GACCATCTCT GCCCCAATCC CCTACGAGGA GCTCGTTCGC 
CTCATTGATG AAGCCAGTGA GAAGAACCTC AGCGTGTCCA TCCTCACCCA GGAGATCATG 
GTGTACTTGG CCATGTACAT CCGCACCCAG CCAGCGCTCT TCACCGATAT GTTTCGAATC 
CGCATTGGGC TCATCATCCA GGTGATGGCA ACAGAGCTGG CGCACTCCCT GCACTGCTCA 
GCTGGAGAAG CCACTGAGAA CCTGATGAAT CTCAGCCCAT CCAACGTGAA GAACCTCCTC 
TACCACATCC TCTCCGGCAA GGAATTTGGA GTGGAGAAGA GTGTGCGATC GGTGGACTCA 
TTGGTCACCA CTGCTATCTC CATCTACGAT GTGGAGGCTG CTGGAGCCAC CAAGACTGAG 
CGTGCTGGCA TCGTCAGGCT GAAAAGTGAG ATCAAACAGC AACTGGATAA ACGTAGGCAG 
TCTCTGACCG GGAGTAAGCC TGGGGACGGT GACCTGCTGG CCTCTCTGTC TGCTGGGCAA 
TCGGAGCTGC TGGACAAAGG CTCCTTTTCA AGCTTAGTGA AAGACCAGAG CAGTAAGGAC 
AGCCGCCAGG GCCAGTGGAA ACGGAGACGG CGGCTGGATG GGGCCCTCAA CCGTGTCCCC 
TTGGGTTTCT ACCAGAAGGT CTGGAAGGTC CTGCAGAAGT GCCACGGTCT TTCCGTGGAG 
GGCTTTGTTC TCCCCTCTTC AACAACCAGA GAGATGACCC CAGGGGAAAT GAAGTTTGCT 
GTGCACGTGG AGTCGGTCCT CAACCGTGTG CCCCAGCCAG AGTACCGGCA GCTGCTGGTA 
GAGACGATCT TGGTGCTCAC CATGCTGGTG GATGTGGAGG TGCACACCAT GGGTGGCATC 
ATACCTGTGG AAAAGATCCT GCACGCAGCC AATGACCTCT TCTATGAGGA GCAGAAGGCC 
CTGGGTGCTG ATGACCAGAT GCTGGAGAAG GATCCCTCGA CGGGCATTTG CGACCTGCTG 
TACGACAGCG CACCGAGTGG CCGCTTCGGC ACCATGACCT ACCTGTCCAA GTCAGTGGCC 
CTGCACCTGT ACGACCTCCT GCCCGGAGAT GGCTGTGCCA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHKA1
Entrez Gene ID	422181
Full Name	phosphorylase kinase regulatory subunit alpha 1
General protein information	Preferred Names phosphorylase kinase regulatory subunit alpha 1 Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform phosphorylase kinase, alpha 1 (muscle)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHKA1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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