TBC1D32 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBC1D32
Entrez Gene ID	421725
Full Name	TBC1 domain family member 32
Synonyms	BROMI,C3H6orf170,C6ORF170,bA301B7.2,bA57L9.1,dJ310J6.1
General protein information	Preferred Names TBC1 domain family member 32 Names protein broad-minded bA301B7.2 (novel protein) broad-minded homolog dJ310J6.1 (novel protein)
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004940263.2	XP_004940320.1	protein broad-minded isoform X2
XM_004940260.2	XP_004940317.1	protein broad-minded isoform X1
XM_025149168.1	XP_025004936.1	protein broad-minded isoform X3
XM_025149169.1	XP_025004937.1	protein broad-minded isoform X5
XM_025149167.1	XP_025004935.1	protein broad-minded isoform X2
XM_004940263.3	XP_004940320.1	protein broad-minded isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	C6H6orf170	XP_001164077.1
Canis lupus familiaris (dog)	C1H6orf170	XP_005615658.1
Danio rerio (zebrafish)	tbc1d32	NP_001093493.1
	LOC714447	XP_002808448.1
Bos taurus (cattle)	TBC1D32	XP_002690121.2
Mus musculus (house mouse)	Tbc1d32	NP_001028557.2
Homo sapiens (human)	TBC1D32	NP_689943.4
Gallus gallus (chicken)	C6ORF170	XP_004940317.1

Gene Ontology

The following TBC1D32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa06565	XM_025149169.1 Latest version!	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa148989	XM_025149167.1 Latest version!	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa148990	XM_025149168.1 Latest version!	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OGa06558	XM_004940260.2 Latest version!	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa06562	XM_004940263.3 Latest version!	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa06562	XM_004940263.2	Gallus gallus TBC1 domain family, member 32 (TBC1D32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBC1D32 ( XM_025149169.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004937.1 Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa06565
Clone ID Related Accession (Same CDS sequence)	XM_025149169.1
Accession Version	XM_025149169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGATGAA 
CTTGTAGTTG AGTTGTCCAC TCAACTTTAT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004937.1
CDS	197..3352
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTHAHYSRTVILRLLEKKYKSLINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQDKDLDELISILQNPGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEDELVVELSTQLYL

Target ORF information:

RefSeq Version	XM_025149169.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149169.1

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGATGAA 
CTTGTAGTTG AGTTGTCCAC TCAACTTTAT CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D32 ( XM_025149167.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004935.1 Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa148989
Clone ID Related Accession (Same CDS sequence)	XM_025149167.1
Accession Version	XM_025149167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGGTGGA CAAACTGAGT GGGCCGAAAT TTGCAGAAGG 
CAATTCTGCA AAATCATGAA AACCAGACCA GATATCATCA GTGGAGCAAT TTTGGCAGAA 
TTAATTGAAA AATTTGTTTT ACATCTTTCT GAGAGTCGAT CGAATTGTTA TTTCAGTATA 
GGAAGATATG AAGCCACAGA TGTAGACATG AAGAAAGAAA GCCTTTCATC GGTGCAGCAG 
CTTGGTATTG AAATGACAGT CAGATATGGA AAATATTTGA ACTTGCTAAA AGAACATGCT 
GAAAATGAGT TGTGCTTCGT GCTAATGAAT TGTGAACAGT TTCTGAAACA ACAGCAAAGA 
ACTGTGGTAT CCTCACTTCG CTGTTTGCAG GGACGCTATG CTGGCTATGA CTGGTTTGCA 
TCTTCTGTGT TCCTCATAAT GTCAGGGGAT GGAGAGAAAA CTCTAACGTT TCTTCAACAA 
TTTTCTCATC TTCTTGTTTC AGCATTTCTC TGGATACCAA GACTGCACAT GTCTATGCAC 
CTGCCTATTA CCACAGTGGA ATCTGGCATC CATCCAGTCT ACTTTTGCAC TGCTCACTAC 
ATTGAGATGT TGCTGAAAGC TGAGTTGCCA CTTGTCTTCT CTGCCTTCCA CATGTCTGGT 
TTCACTCCAT CCCAGATTTG CCTACAATGG ATAACTCAGT GTTTCTGGAA TTACATGGAC 
TGGAGTGAGA TCTGTCATTA CATCGCTACT TGTATTTTCC TTGGTCCTGA CTATCAGGTT 
TATATGTGTA TTTCTGTGTT CAGACACCTA CAGCAAGACA TCCTGAAGCA CACAGAAGCC 
CAAGATCTCC AGGTTTTTCT TAAAGAAGAA GCACTCCATG GATTTCGAGT GACTGATTAT 
CTAGAATACA TGGAAAGCTT GGAGCTGATC TACAGACCAG TGCTGCTGAA AGACATGAGG 
AATATCGGAG TACAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004935.1
CDS	197..4060
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTHAHYSRTVILRLLEKKYKSLINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEATDVDMKKESLSSVQQLGIEMTVRYGKYLNLLKEHAENELCFVLMNCEQFLKQQQRTVVSSLRCLQGRYAGYDWFASSVFLIMSGDGEKTLTFLQQFSHLLVSAFLWIPRLHMSMHLPITTVESGIHPVYFCTAHYIEMLLKAELPLVFSAFHMSGFTPSQICLQWITQCFWNYMDWSEICHYIATCIFLGPDYQVYMCISVFRHLQQDILKHTEAQDLQVFLKEEALHGFRVTDYLEYMESLELIYRPVLLKDMRNIGVQST

Target ORF information:

RefSeq Version	XM_025149167.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149167.1

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGGTGGA CAAACTGAGT GGGCCGAAAT TTGCAGAAGG 
CAATTCTGCA AAATCATGAA AACCAGACCA GATATCATCA GTGGAGCAAT TTTGGCAGAA 
TTAATTGAAA AATTTGTTTT ACATCTTTCT GAGAGTCGAT CGAATTGTTA TTTCAGTATA 
GGAAGATATG AAGCCACAGA TGTAGACATG AAGAAAGAAA GCCTTTCATC GGTGCAGCAG 
CTTGGTATTG AAATGACAGT CAGATATGGA AAATATTTGA ACTTGCTAAA AGAACATGCT 
GAAAATGAGT TGTGCTTCGT GCTAATGAAT TGTGAACAGT TTCTGAAACA ACAGCAAAGA 
ACTGTGGTAT CCTCACTTCG CTGTTTGCAG GGACGCTATG CTGGCTATGA CTGGTTTGCA 
TCTTCTGTGT TCCTCATAAT GTCAGGGGAT GGAGAGAAAA CTCTAACGTT TCTTCAACAA 
TTTTCTCATC TTCTTGTTTC AGCATTTCTC TGGATACCAA GACTGCACAT GTCTATGCAC 
CTGCCTATTA CCACAGTGGA ATCTGGCATC CATCCAGTCT ACTTTTGCAC TGCTCACTAC 
ATTGAGATGT TGCTGAAAGC TGAGTTGCCA CTTGTCTTCT CTGCCTTCCA CATGTCTGGT 
TTCACTCCAT CCCAGATTTG CCTACAATGG ATAACTCAGT GTTTCTGGAA TTACATGGAC 
TGGAGTGAGA TCTGTCATTA CATCGCTACT TGTATTTTCC TTGGTCCTGA CTATCAGGTT 
TATATGTGTA TTTCTGTGTT CAGACACCTA CAGCAAGACA TCCTGAAGCA CACAGAAGCC 
CAAGATCTCC AGGTTTTTCT TAAAGAAGAA GCACTCCATG GATTTCGAGT GACTGATTAT 
CTAGAATACA TGGAAAGCTT GGAGCTGATC TACAGACCAG TGCTGCTGAA AGACATGAGG 
AATATCGGAG TACAGAGCAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D32 ( XM_025149168.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025004936.1 Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X3, mRNA.

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CloneID	OGa148990
Clone ID Related Accession (Same CDS sequence)	XM_025149168.1
Accession Version	XM_025149168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGATT 
TGCCTACAAT GGATAACTCA GTGTTTCTGG AATTACATGG ACTGGAGTGA GATCTGTCAT 
TACATCGCTA CTTGTATTTT CCTTGGTCCT GACTATCAGG TTTATATGTG TATTTCTGTG 
TTCAGACACC TACAGCAAGA CATCCTGAAG CACACAGAAG CCCAAGATCT CCAGGTTTTT 
CTTAAAGAAG AAGCACTCCA TGGATTTCGA GTGACTGATT ATCTAGAATA CATGGAAAGC 
TTGGAGCTGA TCTACAGACC AGTGCTGCTG AAAGACATGA GGAATATCGG AGTACAGAGC 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025004936.1
CDS	197..4042
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQDKDLDELISILQNPGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEATDVDMKKESLSSVQQLGIEMTVRYGKYLNLLKEHAENELCFVLMNCEQFLKQQQRTVVSSLRCLQGRYAGYDWFASSVFLIMSGDGEKTLTFLQQFSHLLVSAFLWIPRLHMSMHLPITTVESGIHPVYFCTAHYIEMLLKAELPLVFSAFHMSGFTPSQICLQWITQCFWNYMDWSEICHYIATCIFLGPDYQVYMCISVFRHLQQDILKHTEAQDLQVFLKEEALHGFRVTDYLEYMESLELIYRPVLLKDMRNIGVQST

Target ORF information:

RefSeq Version	XM_025149168.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025149168.1

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGATT 
TGCCTACAAT GGATAACTCA GTGTTTCTGG AATTACATGG ACTGGAGTGA GATCTGTCAT 
TACATCGCTA CTTGTATTTT CCTTGGTCCT GACTATCAGG TTTATATGTG TATTTCTGTG 
TTCAGACACC TACAGCAAGA CATCCTGAAG CACACAGAAG CCCAAGATCT CCAGGTTTTT 
CTTAAAGAAG AAGCACTCCA TGGATTTCGA GTGACTGATT ATCTAGAATA CATGGAAAGC 
TTGGAGCTGA TCTACAGACC AGTGCTGCTG AAAGACATGA GGAATATCGG AGTACAGAGC 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D32 ( XM_004940260.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940317.1 Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X1, mRNA.

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CloneID	OGa06558
Clone ID Related Accession (Same CDS sequence)	XM_004940260.2
Accession Version	XM_004940260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGATT 
TGCCTACAAT GGATAACTCA GTGTTTCTGG AATTACATGG ACTGGAGTGA GATCTGTCAT 
TACATCGCTA CTTGTATTTT CCTTGGTCCT GACTATCAGG TTTATATGTG TATTTCTGTG 
TTCAGACACC TACAGCAAGA CATCCTGAAG CACACAGAAG CCCAAGATCT CCAGGTTTTT 
CTTAAAGAAG AAGCACTCCA TGGATTTCGA GTGACTGATT ATCTAGAATA CATGGAAAGC 
TTGGAGCTGA TCTACAGACC AGTGCTGCTG AAAGACATGA GGAATATCGG AGTACAGAGC 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940317.1
CDS	197..4102
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTHAHYSRTVILRLLEKKYKSLINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQDKDLDELISILQNPGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEATDVDMKKESLSSVQQLGIEMTVRYGKYLNLLKEHAENELCFVLMNCEQFLKQQQRTVVSSLRCLQGRYAGYDWFASSVFLIMSGDGEKTLTFLQQFSHLLVSAFLWIPRLHMSMHLPITTVESGIHPVYFCTAHYIEMLLKAELPLVFSAFHMSGFTPSQICLQWITQCFWNYMDWSEICHYIATCIFLGPDYQVYMCISVFRHLQQDILKHTEAQDLQVFLKEEALHGFRVTDYLEYMESLELIYRPVLLKDMRNIGVQST

Target ORF information:

RefSeq Version	XM_004940260.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940260.2

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGATT 
TGCCTACAAT GGATAACTCA GTGTTTCTGG AATTACATGG ACTGGAGTGA GATCTGTCAT 
TACATCGCTA CTTGTATTTT CCTTGGTCCT GACTATCAGG TTTATATGTG TATTTCTGTG 
TTCAGACACC TACAGCAAGA CATCCTGAAG CACACAGAAG CCCAAGATCT CCAGGTTTTT 
CTTAAAGAAG AAGCACTCCA TGGATTTCGA GTGACTGATT ATCTAGAATA CATGGAAAGC 
TTGGAGCTGA TCTACAGACC AGTGCTGCTG AAAGACATGA GGAATATCGG AGTACAGAGC 
ACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D32 ( XM_004940263.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940320.1 Gallus gallus TBC1 domain family, member 32 (TBC1D32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa06562
Clone ID Related Accession (Same CDS sequence)	XM_004940263.2 , XM_004940263.3
Accession Version	XM_004940263.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455840.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004940263.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGACA 
CCTACAGCAA GACATCCTGA AGCACACAGA AGCCCAAGAT CTCCAGGTTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940320.1
CDS	206..3862
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTHAHYSRTVILRLLEKKYKSLINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQDKDLDELISILQNPGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEATDVDMKKESLSSVQQLGIEMTVRYGKYLNLLKEHAENELCFVLMNCEQFLKQQQRTVVSSLRCLQGRYAGYDWFASSVFLIMSGDGEKTLTFLQQFSHLLVSAFLWIPRLHMSMHLPITTVESGIHPVYFCTAHYIEMLLKAELPLVFSAFHMSGFTPSQTPTARHPEAHRSPRSPGFS

Target ORF information:

RefSeq Version	XM_004940263.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family, member 32 (TBC1D32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940263.2

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGACA 
CCTACAGCAA GACATCCTGA AGCACACAGA AGCCCAAGAT CTCCAGGTTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBC1D32 ( XM_004940263.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004940320.1 Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X4, mRNA.

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CloneID	OGa06562
Clone ID Related Accession (Same CDS sequence)	XM_004940263.2 , XM_004940263.3
Accession Version	XM_004940263.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein broad-minded isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004940263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGACA 
CCTACAGCAA GACATCCTGA AGCACACAGA AGCCCAAGAT CTCCAGGTTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004940320.1
CDS	197..3853
Translation	MSQFTNEDEALLQSMLRQLLQSVKEKITGAPSVECAEEILLHLEETDENFHNYEFVKYLRQYINNTLGSVIEEESEKCTSTQNQTEISGYDTLVQHVTKKTRESKQYREMMHALKNVMMVVVESLINKFEEDRMCNKELDSKSKKKCQSDYYTDNYSDSDSSFNQSYTFMSQEQLQLLVEKLDPIQPKEVRQEALQMLCLSPPSDVLNSECWPNLRKHLMMSLSDPDAIFSEKILRFYAKTFSSSPFNMAREVYTSLAQHLEFYFFSGEYSLRISSGLDVSNADVNHILKKVRLLNEYQKEAPSSWIRYPEKYMEEIVESTLSLLSVKHDPNHPASPDFLSPIYFLALLDIKAMWFKKWTHAHYSRTVILRLLEKKYKSLINAAVQQCLDYFEYCKAAVSKTPRVSGFSQQHLNNEQKFFYTGSELQYIYFVHSLCLLGRLLIYKQGRNLFPVHLENKKDCISLTDLLVLFARLIYYSPSYSKEALTAHTDNYSPASLVMEILQVFCDQTGCAADCLYTDAVMDALLDPLQSLLNGTEVYINCLETTLIDVADILARIAAAEDGLPVLLYGGSKNSAKESSPTAVHVIVQFTKKLLHDDISLLSGSELLQVVKGAFIFVCRQMYRTCEGLQMLIPYGLHESIGEAWKKASLLSERVPTPVTGADCTSSISLDSKNVLLWEETLLDALLNFSATPKGLFLLHSTGAMSDCVSFMFNSLSKKHQTNEREEFGNGVIVAQMATTPTAAVALQSSGFIKAFITELWALLECGKDDVRITQPKSTPVDPIDQSCQKSFLYLINLLTYPAVFELLSEQDIPNKPMYSHREVPTDIINVIDRIIIVNSEAKIHSLFNYEQSHIFGLRLLSVLCCELNTLLLLETQYKVSQVLLTAQEENVTEASEGPGDFIVDSLSVERNHVLVRINLIGGPQERILPLRALEKGSDPYPWPMFSSFPLPKCYLAEVPRKADFRQDKDLDELISILQNPGGQTEWAEICRRQFCKIMKTRPDIISGAILAELIEKFVLHLSESRSNCYFSIGRYEATDVDMKKESLSSVQQLGIEMTVRYGKYLNLLKEHAENELCFVLMNCEQFLKQQQRTVVSSLRCLQGRYAGYDWFASSVFLIMSGDGEKTLTFLQQFSHLLVSAFLWIPRLHMSMHLPITTVESGIHPVYFCTAHYIEMLLKAELPLVFSAFHMSGFTPSQTPTARHPEAHRSPRSPGFS

Target ORF information:

RefSeq Version	XM_004940263.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus TBC1 domain family member 32 (TBC1D32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004940263.3

ORF Insert Sequence:

ATGTCTCAGT TCACAAATGA AGATGAAGCT TTGTTACAGT CCATGCTTAG ACAATTGCTG 
CAAAGCGTGA AGGAAAAAAT CACTGGGGCT CCGTCAGTGG AATGTGCAGA AGAGATTCTC 
TTGCATTTGG AGGAAACTGA TGAAAATTTT CACAACTATG AATTTGTGAA ATACCTTAGG 
CAGTATATAA ATAATACTCT GGGCTCTGTG ATAGAAGAAG AATCAGAAAA ATGTACTTCT 
ACTCAAAATC AGACTGAAAT TTCTGGCTAT GATACTCTTG TCCAACATGT TACTAAGAAG 
ACACGTGAAT CAAAGCAATA CAGAGAAATG ATGCATGCCT TGAAGAATGT CATGATGGTT 
GTAGTAGAGT CTTTAATTAA CAAGTTTGAA GAAGATCGGA TGTGTAATAA GGAGCTGGAT 
AGTAAAAGCA AGAAAAAATG TCAAAGTGAT TATTATACAG ACAACTATTC TGACAGTGAC 
TCTTCGTTTA ATCAAAGTTA TACATTCATG AGTCAAGAAC AGTTGCAGCT GCTCGTGGAA 
AAGCTTGACC CTATACAGCC TAAGGAGGTT CGCCAAGAAG CATTGCAGAT GCTGTGCTTG 
TCCCCTCCAT CTGATGTATT GAACTCTGAG TGTTGGCCAA ATCTGCGTAA GCACCTGATG 
ATGTCTCTGT CAGATCCTGA TGCAATATTC AGTGAGAAAA TTCTTAGGTT TTATGCGAAA 
ACCTTTTCTT CATCTCCATT TAATATGGCA AGAGAAGTCT ATACAAGTTT AGCACAGCAT 
TTGGAGTTTT ACTTCTTTTC TGGAGAATAT TCTCTTCGTA TTTCATCAGG TCTGGATGTA 
TCTAATGCAG ACGTAAATCA TATACTTAAA AAGGTTCGTC TGCTAAATGA ATACCAAAAG 
GAAGCCCCAT CTTCCTGGAT TCGTTATCCA GAAAAGTACA TGGAGGAAAT AGTGGAAAGT 
ACATTGTCAC TGCTGTCAGT AAAACATGAT CCTAATCATC CTGCCTCTCC AGACTTCTTA 
AGTCCAATCT ACTTCTTGGC TCTGCTGGAT ATCAAGGCTA TGTGGTTTAA AAAGTGGACG 
CATGCACATT ACAGCAGAAC AGTGATACTT AGGCTTTTGG AAAAGAAATA TAAATCTTTG 
ATTAATGCAG CTGTCCAGCA ATGTCTTGAT TATTTTGAAT ATTGCAAGGC AGCAGTTAGT 
AAAACTCCAA GAGTCAGTGG CTTCTCTCAG CAGCATCTTA ATAACGAGCA GAAGTTTTTT 
TATACTGGAT CAGAATTGCA GTACATCTAT TTTGTTCATT CACTATGCCT TTTAGGCAGA 
TTGTTGATTT ATAAGCAAGG CCGAAATCTC TTTCCAGTAC ATCTGGAAAA CAAAAAAGAT 
TGCATATCCC TAACTGATCT GTTGGTATTA TTTGCTCGAC TTATTTATTA CTCTCCAAGT 
TACTCAAAGG AAGCTTTGAC AGCACATACA GACAATTACT CTCCAGCAAG TCTCGTTATG 
GAGATTCTGC AAGTTTTCTG TGATCAAACA GGGTGTGCTG CTGACTGTTT ATATACGGAT 
GCAGTGATGG ATGCCCTACT TGATCCTCTT CAAAGTTTAC TGAATGGTAC AGAGGTATAT 
ATAAATTGCC TTGAAACCAC TTTAATTGAT GTAGCTGATA TTTTGGCAAG GATTGCTGCT 
GCAGAAGATG GTCTTCCTGT ACTTCTTTAT GGAGGAAGTA AAAACTCTGC AAAAGAAAGT 
AGTCCTACAG CTGTTCATGT AATTGTTCAG TTTACAAAGA AACTTCTTCA TGACGACATT 
TCTCTTTTAT CTGGATCCGA GCTTTTGCAA GTTGTTAAAG GAGCTTTCAT TTTTGTATGT 
CGTCAGATGT ACAGAACATG TGAAGGCCTG CAGATGCTAA TTCCTTATGG CTTGCATGAA 
TCAATTGGAG AGGCCTGGAA AAAGGCAAGT TTGCTTTCAG AAAGAGTACC TACTCCTGTA 
ACTGGTGCAG ACTGCACATC ATCAATAAGT CTAGACTCAA AAAATGTTTT GTTATGGGAA 
GAGACTTTGC TAGATGCCTT GCTAAATTTT TCAGCCACAC CTAAAGGACT ATTTTTGCTT 
CATAGTACTG GGGCCATGAG TGATTGTGTG AGCTTTATGT TCAACAGTTT ATCAAAGAAA 
CACCAGACAA ATGAACGTGA AGAATTTGGT AATGGAGTGA TTGTTGCACA AATGGCGACA 
ACACCAACAG CTGCTGTAGC TCTACAGAGT TCAGGGTTTA TAAAGGCCTT TATAACTGAA 
CTGTGGGCTC TTTTGGAGTG TGGAAAAGAT GATGTGAGAA TAACTCAACC CAAATCTACT 
CCAGTTGATC CTATTGACCA AAGCTGTCAG AAGTCTTTTC TGTATCTGAT AAATTTGCTC 
ACTTATCCTG CGGTTTTTGA GCTACTGAGT GAACAGGATA TACCAAATAA ACCAATGTAT 
TCACACCGTG AAGTACCTAC AGACATAATT AACGTCATTG ACAGAATTAT AATTGTGAAC 
TCAGAAGCAA AAATCCATTC CTTATTCAAT TACGAACAAT CGCATATCTT TGGTCTACGG 
TTGTTAAGTG TGCTATGCTG TGAGCTTAAT ACCCTTTTGC TCTTGGAAAC TCAGTATAAA 
GTGTCACAGG TTTTACTGAC TGCTCAGGAA GAAAATGTCA CAGAAGCTTC AGAAGGACCA 
GGAGATTTTA TCGTTGATAG TCTCTCAGTT GAGAGAAACC ATGTTCTTGT CAGAATAAAT 
CTTATTGGAG GACCACAGGA AAGAATTTTG CCTTTGAGAG CGCTAGAAAA GGGCAGTGAT 
CCTTATCCTT GGCCAATGTT TTCATCATTC CCTTTGCCAA AGTGTTATCT TGCAGAAGTT 
CCTAGGAAAG CTGATTTCAG ACAAGATAAA GATCTTGACG AGTTGATATC AATCTTACAA 
AATCCAGGTG GACAAACTGA GTGGGCCGAA ATTTGCAGAA GGCAATTCTG CAAAATCATG 
AAAACCAGAC CAGATATCAT CAGTGGAGCA ATTTTGGCAG AATTAATTGA AAAATTTGTT 
TTACATCTTT CTGAGAGTCG ATCGAATTGT TATTTCAGTA TAGGAAGATA TGAAGCCACA 
GATGTAGACA TGAAGAAAGA AAGCCTTTCA TCGGTGCAGC AGCTTGGTAT TGAAATGACA 
GTCAGATATG GAAAATATTT GAACTTGCTA AAAGAACATG CTGAAAATGA GTTGTGCTTC 
GTGCTAATGA ATTGTGAACA GTTTCTGAAA CAACAGCAAA GAACTGTGGT ATCCTCACTT 
CGCTGTTTGC AGGGACGCTA TGCTGGCTAT GACTGGTTTG CATCTTCTGT GTTCCTCATA 
ATGTCAGGGG ATGGAGAGAA AACTCTAACG TTTCTTCAAC AATTTTCTCA TCTTCTTGTT 
TCAGCATTTC TCTGGATACC AAGACTGCAC ATGTCTATGC ACCTGCCTAT TACCACAGTG 
GAATCTGGCA TCCATCCAGT CTACTTTTGC ACTGCTCACT ACATTGAGAT GTTGCTGAAA 
GCTGAGTTGC CACTTGTCTT CTCTGCCTTC CACATGTCTG GTTTCACTCC ATCCCAGACA 
CCTACAGCAA GACATCCTGA AGCACACAGA AGCCCAAGAT CTCCAGGTTT TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TBC1D32 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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