tbc1d32 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tbc1d32
Entrez Gene ID	566474
Full Name	TBC1 domain family, member 32
Synonyms	si:dkey-233p4.1,si:dkey233p4.1
General protein information	Preferred Names TBC1 domain family, member 32 Names protein broad-minded bromi
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170186.3	XP_005170243.1	protein broad-minded isoform X1
XM_021468491.1	XP_021324166.1	protein broad-minded isoform X1
XM_009294920.2	XP_009293195.1	protein broad-minded isoform X1
XM_009294920.3	XP_009293195.1	protein broad-minded isoform X1
NM_001100023.1	NP_001093493.1	protein broad-minded

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	C6H6orf170	XP_001164077.1
Canis lupus familiaris (dog)	C1H6orf170	XP_005615658.1
Danio rerio (zebrafish)	tbc1d32	NP_001093493.1
	LOC714447	XP_002808448.1
Bos taurus (cattle)	TBC1D32	XP_002690121.2
Mus musculus (house mouse)	Tbc1d32	NP_001028557.2
Homo sapiens (human)	TBC1D32	NP_689943.4
Gallus gallus (chicken)	C6ORF170	XP_004940317.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction.
Ko HW, Norman RX, Tran J, Fuller KP, Fukuda M, Eggenschwiler JT
Developmental cell18(2)237-47(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tbc1d32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa04087	NM_001100023.1 Latest version!	Danio rerio TBC1 domain family, member 32 (tbc1d32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa25180	XM_009294920.3 Latest version!	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa25180	XM_021468491.1 Latest version!	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa25180	XM_005170186.3 Latest version!	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa25180	XM_009294920.2	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tbc1d32 ( NM_001100023.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093493.1 Danio rerio TBC1 domain family, member 32 (tbc1d32), mRNA.

Price & Availability↑

CloneID	ODa04087
Clone ID Related Accession (Same CDS sequence)	NM_001100023.1
Accession Version	NM_001100023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein broad-minded
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT955964.5, BX470129.4 and BX470071.4. On Jul 26, 2007 this sequence version replaced XM_689744.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGGTAATACA 
AAAACCTTGG AGGCTCATTT CCTTTACCAC AAGTTGAGTT TTCCCAGTGG CACTACTAGT 
ATTGATGCAA ACAGACCAGA TATGGCCCGT CTCCTCAAAC AAATGCGATT AATGAATGAC 
TTTCAAAAGG AAGTGACTAC GTTTTGGATA CGGCATCCTG AGAAGTACAT GGAAGAAATC 
ATTGAAAACA CCCTGTCTCT TCTCGCTCTG CATTCTGAAC AGGGCATGAG CAGTCCCGGC 
TCAGAGAAAT CCCTAGAGCC TATTCACTTA ATCTCTCTGC TGGATATCAA AGCCACCTGG 
TTTAAGAAAT GGATGCATGG TTACTATAGC AGAACTGTTG TCTTAAGACT TCTGGAGAGG 
AAATACAAGT CTTTGATTGT TAATGCTCTG CAGCAGTGCA TTTATTACTT TGATTCCTGT 
GATGCATTAA GTGAAGAGAC CTTGGAAATG ATCCACAGCC TGGAACATCA GCAGATTGGC 
CCCGCTGGAA GAACTCTCTA CACATCTAAA GAGCTGGAGT TTGTTTACTT CGTTCATTCG 
CTGTGTGTTC TCGGGAGGCT TGTGATGTAC ACTAATGGAA GGAAATTTTT TCCTATTAAA 
GTGAAGAAGC GAAGAGATCC GGTGACTTTG ACCGACTTAG TCGTGATTCT GATCAATATA 
ATGTACCAAC ACCCAAAACC ATCCTGCGGT GAGACGGCAC TTGCAGACAC CATGTCCCCC 
ACTAATCTTG TGATGGAGGT GTTAAGGACC CTGTGTGACC GCACTGAGTG TGCTGTGGAG 
TGTCTTTACC AAATCCCTGT TATTGAGACT CTGCTGGCTC CTCTTATCAC TCTGCTCACA 
GGAAAGCTGC CAAAATTAAA TTCCATGGAA AGTGCCCTGA CTCACACAGC AGACACGCTG 
GCGAGGATTG CTACCACAGA ACGAGGTTTA TCACTCCTAC TTTATGACAG AAATCTCGTC 
TCGGCCGAGG GTGAAAGCAT TTCAGCAGCA CATCTAATAG TGCAGTTTAC CCAGAAGCTG 
TTGGCAAAGG AGCTGCAAGT GGAGAATTCT TCTAGCATGA GCGGGGCCTT CATCTTCGTC 
TGTCGGCAGA TGTACAACAC CTGCGAGGGC CTGCAAGTTC TGCGGCCATA CAGCCTGCAT 
GAGTGCATTG CTCAAGCCTG GAGAACGACA AGCTCAATGT CGGAAAGAGT TCCCACTCCT 
GTTCCTGGAG CTCCCCCAGC ACTGGCGTCC CAGGAGCTGC AGAGCGTGGT GGCCTGGGAA 
GAGATGCTGT TAGACAACCT GTTGAATTTC GCAGCCACAC CAAAAGGACT TTTGCTTCTT 
CAACAGACCG GGGCCATTAA TGAGTGTGTC ACTTACATGT TTTCAAGATT CACCAAGAAA 
CTGCAGGTCA GCAGATGTGA GAAGTTTGGT TATGGTGTTA TGGTGACACA AGTGGCCACT 
ACAGCCCCTG GAATTGTGGC CTTGCAAAGC TCAGGATTTG TCCAGACAAT TGTGGTGGAG 
CTGTGGTCAG CCTTGGAATG TGGACGAGAG GATGTAAGGG TGGTCCATCC AAAATCTACT 
CCAATGGACC CAATTGACAG GAGCTGCCTC AAGTCCTTCC TGTCTCTAGT GAATCTGCTT 
TCTTCACCTC ACGCTGTGTG GGAGCTTCTG GGACATCGAG CACTTCCTAA CAAGACGGAG 
TACAATCTGA GAGAGATGGC CACATCCGTT TTAGACCTGA TGGACCGTCT CATCATTATT 
AACTCTGACG CGAAGATTCA CTCTCTTTTC AACTATGAAC AGTCCCACAC GTTTGGCCTG 
AGACTCCTGA GTGTGCTTTG CTGTAACCTG GATTCTTTTC TGCTGCTGGA GTCTCAGTAT 
AAACTTACTG ACTTGCTGCT TCAGGGTCAG AGGGACAATG TCACTGAGCC ACCATCAGGT 
GAAGGGGAAT TCATCATTGA TGGACTGTCT GTGGAAAGAA ACCATATCCT AGTTCGAATC 
TGTGCAGTTG GCGGTCCATC TGAGAGAAAA CTTCCCCCCA GAGCCTTGCA GAAAGGGAAT 
GACCCTTACC CATGGCCAAT GGTGTCCACT TATCCTCTGC CTAAGTATTA TATATTCGAC 
GTGCCCAAGA TTCTCCGCAC TAAACAGGAG TCAGAAATTG GTGCTTTCCT CACCTCATCT 
AAGGAAACAG AGAGAGATGC TAGCTGGATG GATGACTGCA GGAGACAGTT CTGCAAGATT 
ATGGCGACAA AGTCAAACAC TCTCACAGGA TACGTGCTTG CCGATCTGTT GGAGATGGTC 
GTTCTGCATC TCTCGTCTTC ATCCAATGAC TGCTTCTTTC CGCCTGCAGA ATATAAAGTG 
GTGGATAACA GTGTAAAGAC TCGAAGCCTG TCATCTGTGG AGCAGCTAGG TGTGGAAATC 
TCACTCAGGT ATGGCAAGTT TCTGAAGCTG CTGAGAGAAG ATTCAGAGCA GGATCTGTGT 
CTTCTGCTGA AACACTGTCA GGAGTTTCTC TCTCAGCAAC GTGTCAAGGT CACCTCTGAG 
CTCTGTGGTT ATCCTGGTCA CGATTGGTTT GCTTCTACAG TATTTCTGCT CATGCACGGT 
GATGTGGGCC GCTCTCTGAG TTTGCTCTTG CGTTTCTCCC GTCTCCTGCC GTCTGCCTTC 
CTGTGGCCTC CGAGACTGCA CAGCTCTGTC CATCTCCCTA TAGAGATTGC TCAGTCGGCT 
ATTCATCCAA TCTACTCCTG CACCGCTCAT TATGTAGAGA TGTTGCTCAA GGCTGAGGTT 
CCTCTGGTTT TTTCTGCCTT CAGAATGTCT GGCTTCACTC CCTCTCAGAT CTGTGTTCAG 
TGGCTCGGCC AGTGCTTCTG GAACTACCTG GACTGGCCTG AGATCTGTCA GTATGTGACC 
ACCTGCGTCA TTATGGGACC CGATTACCAG GTGTACATGT GCGTCTCTGC TCTCCGTCAC 
CTACAGCAGG ACATCCTACA GCACACACAG ACACAAGACC TCCAGGTCTT CCTCAAGGAG 
GAACCAATCC AAGGCTTCAG AGTGAGTAAT TACCTGGAGT ACATGGAGGG GCTGGAGCGC 
AGCTACAGAT CCATGGTGCT CTCGGACATG AGGAGCATCT TACCGAGGAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093493.1
CDS	1..3897
Translation	MSQFSTEDEAELQTLLRKLLKSVKDRISGAPSVECAEEILLHLEETDKNFHNYEFVKYLREYVESSLGAVIEVETENFTKGEGHAVGSGQDTLVHAVTKNTRESTQYKQMMQTLKQTMMMVVESLINKFEEDQMKREEMDRKIQHQQSISQYADNCSDSDSSFNQSYAFIKHEQLQVIAEKLDPGRPREVRWEALQSLCHAPPSDVLSCESWTGLRRNLSTALTDPDPELSDKVLQFFAKTFSSSPLNVTKDIYASIGNTKTLEAHFLYHKLSFPSGTTSIDANRPDMARLLKQMRLMNDFQKEVTTFWIRHPEKYMEEIIENTLSLLALHSEQGMSSPGSEKSLEPIHLISLLDIKATWFKKWMHGYYSRTVVLRLLERKYKSLIVNALQQCIYYFDSCDALSEETLEMIHSLEHQQIGPAGRTLYTSKELEFVYFVHSLCVLGRLVMYTNGRKFFPIKVKKRRDPVTLTDLVVILINIMYQHPKPSCGETALADTMSPTNLVMEVLRTLCDRTECAVECLYQIPVIETLLAPLITLLTGKLPKLNSMESALTHTADTLARIATTERGLSLLLYDRNLVSAEGESISAAHLIVQFTQKLLAKELQVENSSSMSGAFIFVCRQMYNTCEGLQVLRPYSLHECIAQAWRTTSSMSERVPTPVPGAPPALASQELQSVVAWEEMLLDNLLNFAATPKGLLLLQQTGAINECVTYMFSRFTKKLQVSRCEKFGYGVMVTQVATTAPGIVALQSSGFVQTIVVELWSALECGREDVRVVHPKSTPMDPIDRSCLKSFLSLVNLLSSPHAVWELLGHRALPNKTEYNLREMATSVLDLMDRLIIINSDAKIHSLFNYEQSHTFGLRLLSVLCCNLDSFLLLESQYKLTDLLLQGQRDNVTEPPSGEGEFIIDGLSVERNHILVRICAVGGPSERKLPPRALQKGNDPYPWPMVSTYPLPKYYIFDVPKILRTKQESEIGAFLTSSKETERDASWMDDCRRQFCKIMATKSNTLTGYVLADLLEMVVLHLSSSSNDCFFPPAEYKVVDNSVKTRSLSSVEQLGVEISLRYGKFLKLLREDSEQDLCLLLKHCQEFLSQQRVKVTSELCGYPGHDWFASTVFLLMHGDVGRSLSLLLRFSRLLPSAFLWPPRLHSSVHLPIEIAQSAIHPIYSCTAHYVEMLLKAEVPLVFSAFRMSGFTPSQICVQWLGQCFWNYLDWPEICQYVTTCVIMGPDYQVYMCVSALRHLQQDILQHTQTQDLQVFLKEEPIQGFRVSNYLEYMEGLERSYRSMVLSDMRSILPRSS

Target ORF information:

RefSeq Version	NM_001100023.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain family, member 32 (tbc1d32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100023.1

ORF Insert Sequence:

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGGTAATACA 
AAAACCTTGG AGGCTCATTT CCTTTACCAC AAGTTGAGTT TTCCCAGTGG CACTACTAGT 
ATTGATGCAA ACAGACCAGA TATGGCCCGT CTCCTCAAAC AAATGCGATT AATGAATGAC 
TTTCAAAAGG AAGTGACTAC GTTTTGGATA CGGCATCCTG AGAAGTACAT GGAAGAAATC 
ATTGAAAACA CCCTGTCTCT TCTCGCTCTG CATTCTGAAC AGGGCATGAG CAGTCCCGGC 
TCAGAGAAAT CCCTAGAGCC TATTCACTTA ATCTCTCTGC TGGATATCAA AGCCACCTGG 
TTTAAGAAAT GGATGCATGG TTACTATAGC AGAACTGTTG TCTTAAGACT TCTGGAGAGG 
AAATACAAGT CTTTGATTGT TAATGCTCTG CAGCAGTGCA TTTATTACTT TGATTCCTGT 
GATGCATTAA GTGAAGAGAC CTTGGAAATG ATCCACAGCC TGGAACATCA GCAGATTGGC 
CCCGCTGGAA GAACTCTCTA CACATCTAAA GAGCTGGAGT TTGTTTACTT CGTTCATTCG 
CTGTGTGTTC TCGGGAGGCT TGTGATGTAC ACTAATGGAA GGAAATTTTT TCCTATTAAA 
GTGAAGAAGC GAAGAGATCC GGTGACTTTG ACCGACTTAG TCGTGATTCT GATCAATATA 
ATGTACCAAC ACCCAAAACC ATCCTGCGGT GAGACGGCAC TTGCAGACAC CATGTCCCCC 
ACTAATCTTG TGATGGAGGT GTTAAGGACC CTGTGTGACC GCACTGAGTG TGCTGTGGAG 
TGTCTTTACC AAATCCCTGT TATTGAGACT CTGCTGGCTC CTCTTATCAC TCTGCTCACA 
GGAAAGCTGC CAAAATTAAA TTCCATGGAA AGTGCCCTGA CTCACACAGC AGACACGCTG 
GCGAGGATTG CTACCACAGA ACGAGGTTTA TCACTCCTAC TTTATGACAG AAATCTCGTC 
TCGGCCGAGG GTGAAAGCAT TTCAGCAGCA CATCTAATAG TGCAGTTTAC CCAGAAGCTG 
TTGGCAAAGG AGCTGCAAGT GGAGAATTCT TCTAGCATGA GCGGGGCCTT CATCTTCGTC 
TGTCGGCAGA TGTACAACAC CTGCGAGGGC CTGCAAGTTC TGCGGCCATA CAGCCTGCAT 
GAGTGCATTG CTCAAGCCTG GAGAACGACA AGCTCAATGT CGGAAAGAGT TCCCACTCCT 
GTTCCTGGAG CTCCCCCAGC ACTGGCGTCC CAGGAGCTGC AGAGCGTGGT GGCCTGGGAA 
GAGATGCTGT TAGACAACCT GTTGAATTTC GCAGCCACAC CAAAAGGACT TTTGCTTCTT 
CAACAGACCG GGGCCATTAA TGAGTGTGTC ACTTACATGT TTTCAAGATT CACCAAGAAA 
CTGCAGGTCA GCAGATGTGA GAAGTTTGGT TATGGTGTTA TGGTGACACA AGTGGCCACT 
ACAGCCCCTG GAATTGTGGC CTTGCAAAGC TCAGGATTTG TCCAGACAAT TGTGGTGGAG 
CTGTGGTCAG CCTTGGAATG TGGACGAGAG GATGTAAGGG TGGTCCATCC AAAATCTACT 
CCAATGGACC CAATTGACAG GAGCTGCCTC AAGTCCTTCC TGTCTCTAGT GAATCTGCTT 
TCTTCACCTC ACGCTGTGTG GGAGCTTCTG GGACATCGAG CACTTCCTAA CAAGACGGAG 
TACAATCTGA GAGAGATGGC CACATCCGTT TTAGACCTGA TGGACCGTCT CATCATTATT 
AACTCTGACG CGAAGATTCA CTCTCTTTTC AACTATGAAC AGTCCCACAC GTTTGGCCTG 
AGACTCCTGA GTGTGCTTTG CTGTAACCTG GATTCTTTTC TGCTGCTGGA GTCTCAGTAT 
AAACTTACTG ACTTGCTGCT TCAGGGTCAG AGGGACAATG TCACTGAGCC ACCATCAGGT 
GAAGGGGAAT TCATCATTGA TGGACTGTCT GTGGAAAGAA ACCATATCCT AGTTCGAATC 
TGTGCAGTTG GCGGTCCATC TGAGAGAAAA CTTCCCCCCA GAGCCTTGCA GAAAGGGAAT 
GACCCTTACC CATGGCCAAT GGTGTCCACT TATCCTCTGC CTAAGTATTA TATATTCGAC 
GTGCCCAAGA TTCTCCGCAC TAAACAGGAG TCAGAAATTG GTGCTTTCCT CACCTCATCT 
AAGGAAACAG AGAGAGATGC TAGCTGGATG GATGACTGCA GGAGACAGTT CTGCAAGATT 
ATGGCGACAA AGTCAAACAC TCTCACAGGA TACGTGCTTG CCGATCTGTT GGAGATGGTC 
GTTCTGCATC TCTCGTCTTC ATCCAATGAC TGCTTCTTTC CGCCTGCAGA ATATAAAGTG 
GTGGATAACA GTGTAAAGAC TCGAAGCCTG TCATCTGTGG AGCAGCTAGG TGTGGAAATC 
TCACTCAGGT ATGGCAAGTT TCTGAAGCTG CTGAGAGAAG ATTCAGAGCA GGATCTGTGT 
CTTCTGCTGA AACACTGTCA GGAGTTTCTC TCTCAGCAAC GTGTCAAGGT CACCTCTGAG 
CTCTGTGGTT ATCCTGGTCA CGATTGGTTT GCTTCTACAG TATTTCTGCT CATGCACGGT 
GATGTGGGCC GCTCTCTGAG TTTGCTCTTG CGTTTCTCCC GTCTCCTGCC GTCTGCCTTC 
CTGTGGCCTC CGAGACTGCA CAGCTCTGTC CATCTCCCTA TAGAGATTGC TCAGTCGGCT 
ATTCATCCAA TCTACTCCTG CACCGCTCAT TATGTAGAGA TGTTGCTCAA GGCTGAGGTT 
CCTCTGGTTT TTTCTGCCTT CAGAATGTCT GGCTTCACTC CCTCTCAGAT CTGTGTTCAG 
TGGCTCGGCC AGTGCTTCTG GAACTACCTG GACTGGCCTG AGATCTGTCA GTATGTGACC 
ACCTGCGTCA TTATGGGACC CGATTACCAG GTGTACATGT GCGTCTCTGC TCTCCGTCAC 
CTACAGCAGG ACATCCTACA GCACACACAG ACACAAGACC TCCAGGTCTT CCTCAAGGAG 
GAACCAATCC AAGGCTTCAG AGTGAGTAAT TACCTGGAGT ACATGGAGGG GCTGGAGCGC 
AGCTACAGAT CCATGGTGCT CTCGGACATG AGGAGCATCT TACCGAGGAG CAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d32 ( XM_005170186.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170243.1 Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25180
Clone ID Related Accession (Same CDS sequence)	XM_005170186.3 , XM_021468491.1 , XM_009294920.2 , XM_009294920.3
Accession Version	XM_005170186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein broad-minded isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170186.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170243.1
CDS	390..4292
Translation	MSQFSTEDEAELQTLLRKLLKSVKDRISGAPSVECAEEILLHLEETDKNFHNYEFVKYLREYVESSLGAVIEVETENFTKGEGHAVGSGQDTLVHAVTKNTRESTQYKQMMQTLKQTMMMVVESLINKFEEDQMKREEMDRKIQHQQSISQYADNCSDSDSSFNQSYAFIKHEQLQVIAEKLDPGRPREVRWEALQSLCHAPPSDVLSCESWTGLRRNLSTALTDPDPELSDKVLQFFAKTFSSSPLNVTKDIYASIAKTLEAHFLYHKLSFPSGTTSIDANRPDMARLLKQMRLMNDFQKEVTTFWIRHPEKYMEEIIENTLSLLALHSEQGMSSPGSEKSLEPIHLISLLDIKATWFKKWMHGYYSRTVVLRLLERKYKSLIVNALQQCIYYFDSCDALSEETLEMIHSLEHQQIGPAGRTLYTSKELEFVYFVHSLCVLGRLVMYTNGRKFFPIKVKKRRDPVTLTDLVVILINIMYQHPKPSCGETALADTMSPTNLVMEVLRTLCDRTECAVECLYQIPVIETLLAPLITLLTGKLPKLNSMESALTHTADTLARIATTERGLSLLLYDRNLVSAEGESISAAHLIVQFTQKLLAKELQVENSSSMSGAFIFVCRQMYNTCEGLQVLRPYSLHECIAQAWRTTSSMSERVPTPVPGAPPALASQELQSVVAWEEMLLDNLLNFAATPKGLLLLQQTGAINECVTYMFSRFTKKLQVSRCEKFGYGVMVTQVATTAPGIVALQSSGFVQTIVVELWSALECGREDVRVVHPKSTPMDPIDRSCLKSFLSLVNLLSSPHAVWELLGHRALPNKTEYNLREMATSVLDLMDRLIIINSDAKIHSLFNYEQSHTFGLRLLSVLCCNLDSFLLLESQYKLTDLLLQGQRDNVTEPPSGEGEFIIDGLSVERNHILVRICAVGGPSERKLPPRALQKGNDPYPWPMVSTYPLPKYYIFDVPKILRTKQESEIGAFLTSSKETERDASWMDDCRRQFCKIMATKSNTLTGYVLADLLEMVVLHLSSSSNDCFFPPAEYKVVDNSVKTRSLSSVEQLGVEISLRYGKFLKLLREDSEQDLCLLLKHCQEFLSQQRVKVTSELSHFQGGYPGHDWFASTVFLLMHGDVGRSLSLLLRFSRLLPSAFLWPPRLHSSVHLPIEIAQSAIHPIYSCTAHYVEMLLKAEVPLVFSAFRMSGFTPSQICVQWLGQCFWNYLDWPEICQYVTTCVIMGPDYQVYMCVSALRHLQQDILQHTQTQDLQVFLKEEPIQGFRVSNYLEYMEGLERSYRSMVLSDMRSILPRSS

Target ORF information:

RefSeq Version	XM_005170186.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170186.3

ORF Insert Sequence:

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d32 ( XM_021468491.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324166.1 Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.

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CloneID	ODa25180
Clone ID Related Accession (Same CDS sequence)	XM_005170186.3 , XM_021468491.1 , XM_009294920.2 , XM_009294920.3
Accession Version	XM_021468491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein broad-minded isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324166.1
CDS	431..4333
Translation	MSQFSTEDEAELQTLLRKLLKSVKDRISGAPSVECAEEILLHLEETDKNFHNYEFVKYLREYVESSLGAVIEVETENFTKGEGHAVGSGQDTLVHAVTKNTRESTQYKQMMQTLKQTMMMVVESLINKFEEDQMKREEMDRKIQHQQSISQYADNCSDSDSSFNQSYAFIKHEQLQVIAEKLDPGRPREVRWEALQSLCHAPPSDVLSCESWTGLRRNLSTALTDPDPELSDKVLQFFAKTFSSSPLNVTKDIYASIAKTLEAHFLYHKLSFPSGTTSIDANRPDMARLLKQMRLMNDFQKEVTTFWIRHPEKYMEEIIENTLSLLALHSEQGMSSPGSEKSLEPIHLISLLDIKATWFKKWMHGYYSRTVVLRLLERKYKSLIVNALQQCIYYFDSCDALSEETLEMIHSLEHQQIGPAGRTLYTSKELEFVYFVHSLCVLGRLVMYTNGRKFFPIKVKKRRDPVTLTDLVVILINIMYQHPKPSCGETALADTMSPTNLVMEVLRTLCDRTECAVECLYQIPVIETLLAPLITLLTGKLPKLNSMESALTHTADTLARIATTERGLSLLLYDRNLVSAEGESISAAHLIVQFTQKLLAKELQVENSSSMSGAFIFVCRQMYNTCEGLQVLRPYSLHECIAQAWRTTSSMSERVPTPVPGAPPALASQELQSVVAWEEMLLDNLLNFAATPKGLLLLQQTGAINECVTYMFSRFTKKLQVSRCEKFGYGVMVTQVATTAPGIVALQSSGFVQTIVVELWSALECGREDVRVVHPKSTPMDPIDRSCLKSFLSLVNLLSSPHAVWELLGHRALPNKTEYNLREMATSVLDLMDRLIIINSDAKIHSLFNYEQSHTFGLRLLSVLCCNLDSFLLLESQYKLTDLLLQGQRDNVTEPPSGEGEFIIDGLSVERNHILVRICAVGGPSERKLPPRALQKGNDPYPWPMVSTYPLPKYYIFDVPKILRTKQESEIGAFLTSSKETERDASWMDDCRRQFCKIMATKSNTLTGYVLADLLEMVVLHLSSSSNDCFFPPAEYKVVDNSVKTRSLSSVEQLGVEISLRYGKFLKLLREDSEQDLCLLLKHCQEFLSQQRVKVTSELSHFQGGYPGHDWFASTVFLLMHGDVGRSLSLLLRFSRLLPSAFLWPPRLHSSVHLPIEIAQSAIHPIYSCTAHYVEMLLKAEVPLVFSAFRMSGFTPSQICVQWLGQCFWNYLDWPEICQYVTTCVIMGPDYQVYMCVSALRHLQQDILQHTQTQDLQVFLKEEPIQGFRVSNYLEYMEGLERSYRSMVLSDMRSILPRSS

Target ORF information:

RefSeq Version	XM_021468491.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468491.1

ORF Insert Sequence:

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d32 ( XM_009294920.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293195.1 Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25180
Clone ID Related Accession (Same CDS sequence)	XM_005170186.3 , XM_021468491.1 , XM_009294920.2 , XM_009294920.3
Accession Version	XM_009294920.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein broad-minded isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293195.1
CDS	432..4334
Translation	MSQFSTEDEAELQTLLRKLLKSVKDRISGAPSVECAEEILLHLEETDKNFHNYEFVKYLREYVESSLGAVIEVETENFTKGEGHAVGSGQDTLVHAVTKNTRESTQYKQMMQTLKQTMMMVVESLINKFEEDQMKREEMDRKIQHQQSISQYADNCSDSDSSFNQSYAFIKHEQLQVIAEKLDPGRPREVRWEALQSLCHAPPSDVLSCESWTGLRRNLSTALTDPDPELSDKVLQFFAKTFSSSPLNVTKDIYASIAKTLEAHFLYHKLSFPSGTTSIDANRPDMARLLKQMRLMNDFQKEVTTFWIRHPEKYMEEIIENTLSLLALHSEQGMSSPGSEKSLEPIHLISLLDIKATWFKKWMHGYYSRTVVLRLLERKYKSLIVNALQQCIYYFDSCDALSEETLEMIHSLEHQQIGPAGRTLYTSKELEFVYFVHSLCVLGRLVMYTNGRKFFPIKVKKRRDPVTLTDLVVILINIMYQHPKPSCGETALADTMSPTNLVMEVLRTLCDRTECAVECLYQIPVIETLLAPLITLLTGKLPKLNSMESALTHTADTLARIATTERGLSLLLYDRNLVSAEGESISAAHLIVQFTQKLLAKELQVENSSSMSGAFIFVCRQMYNTCEGLQVLRPYSLHECIAQAWRTTSSMSERVPTPVPGAPPALASQELQSVVAWEEMLLDNLLNFAATPKGLLLLQQTGAINECVTYMFSRFTKKLQVSRCEKFGYGVMVTQVATTAPGIVALQSSGFVQTIVVELWSALECGREDVRVVHPKSTPMDPIDRSCLKSFLSLVNLLSSPHAVWELLGHRALPNKTEYNLREMATSVLDLMDRLIIINSDAKIHSLFNYEQSHTFGLRLLSVLCCNLDSFLLLESQYKLTDLLLQGQRDNVTEPPSGEGEFIIDGLSVERNHILVRICAVGGPSERKLPPRALQKGNDPYPWPMVSTYPLPKYYIFDVPKILRTKQESEIGAFLTSSKETERDASWMDDCRRQFCKIMATKSNTLTGYVLADLLEMVVLHLSSSSNDCFFPPAEYKVVDNSVKTRSLSSVEQLGVEISLRYGKFLKLLREDSEQDLCLLLKHCQEFLSQQRVKVTSELSHFQGGYPGHDWFASTVFLLMHGDVGRSLSLLLRFSRLLPSAFLWPPRLHSSVHLPIEIAQSAIHPIYSCTAHYVEMLLKAEVPLVFSAFRMSGFTPSQICVQWLGQCFWNYLDWPEICQYVTTCVIMGPDYQVYMCVSALRHLQQDILQHTQTQDLQVFLKEEPIQGFRVSNYLEYMEGLERSYRSMVLSDMRSILPRSS

Target ORF information:

RefSeq Version	XM_009294920.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294920.2

ORF Insert Sequence:

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tbc1d32 ( XM_009294920.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293195.1 Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25180
Clone ID Related Accession (Same CDS sequence)	XM_005170186.3 , XM_021468491.1 , XM_009294920.2 , XM_009294920.3
Accession Version	XM_009294920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein broad-minded isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293195.1
CDS	475..4377
Translation	MSQFSTEDEAELQTLLRKLLKSVKDRISGAPSVECAEEILLHLEETDKNFHNYEFVKYLREYVESSLGAVIEVETENFTKGEGHAVGSGQDTLVHAVTKNTRESTQYKQMMQTLKQTMMMVVESLINKFEEDQMKREEMDRKIQHQQSISQYADNCSDSDSSFNQSYAFIKHEQLQVIAEKLDPGRPREVRWEALQSLCHAPPSDVLSCESWTGLRRNLSTALTDPDPELSDKVLQFFAKTFSSSPLNVTKDIYASIAKTLEAHFLYHKLSFPSGTTSIDANRPDMARLLKQMRLMNDFQKEVTTFWIRHPEKYMEEIIENTLSLLALHSEQGMSSPGSEKSLEPIHLISLLDIKATWFKKWMHGYYSRTVVLRLLERKYKSLIVNALQQCIYYFDSCDALSEETLEMIHSLEHQQIGPAGRTLYTSKELEFVYFVHSLCVLGRLVMYTNGRKFFPIKVKKRRDPVTLTDLVVILINIMYQHPKPSCGETALADTMSPTNLVMEVLRTLCDRTECAVECLYQIPVIETLLAPLITLLTGKLPKLNSMESALTHTADTLARIATTERGLSLLLYDRNLVSAEGESISAAHLIVQFTQKLLAKELQVENSSSMSGAFIFVCRQMYNTCEGLQVLRPYSLHECIAQAWRTTSSMSERVPTPVPGAPPALASQELQSVVAWEEMLLDNLLNFAATPKGLLLLQQTGAINECVTYMFSRFTKKLQVSRCEKFGYGVMVTQVATTAPGIVALQSSGFVQTIVVELWSALECGREDVRVVHPKSTPMDPIDRSCLKSFLSLVNLLSSPHAVWELLGHRALPNKTEYNLREMATSVLDLMDRLIIINSDAKIHSLFNYEQSHTFGLRLLSVLCCNLDSFLLLESQYKLTDLLLQGQRDNVTEPPSGEGEFIIDGLSVERNHILVRICAVGGPSERKLPPRALQKGNDPYPWPMVSTYPLPKYYIFDVPKILRTKQESEIGAFLTSSKETERDASWMDDCRRQFCKIMATKSNTLTGYVLADLLEMVVLHLSSSSNDCFFPPAEYKVVDNSVKTRSLSSVEQLGVEISLRYGKFLKLLREDSEQDLCLLLKHCQEFLSQQRVKVTSELSHFQGGYPGHDWFASTVFLLMHGDVGRSLSLLLRFSRLLPSAFLWPPRLHSSVHLPIEIAQSAIHPIYSCTAHYVEMLLKAEVPLVFSAFRMSGFTPSQICVQWLGQCFWNYLDWPEICQYVTTCVIMGPDYQVYMCVSALRHLQQDILQHTQTQDLQVFLKEEPIQGFRVSNYLEYMEGLERSYRSMVLSDMRSILPRSS

Target ORF information:

RefSeq Version	XM_009294920.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain family, member 32 (tbc1d32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294920.3

ORF Insert Sequence:

ATGTCTCAGT TCTCCACCGA GGATGAGGCA GAGCTCCAGA CCCTGCTGCG AAAGCTCCTG 
AAAAGTGTCA AAGACAGAAT CTCAGGAGCA CCATCGGTAG AATGTGCTGA GGAGATCCTG 
CTTCATTTGG AGGAAACTGA CAAGAACTTC CACAACTATG AGTTTGTCAA GTATCTAAGG 
GAGTATGTGG AGAGCAGCTT GGGAGCAGTT ATTGAAGTGG AGACTGAGAA TTTTACTAAG 
GGTGAAGGTC ATGCTGTTGG CTCTGGGCAG GACACCTTGG TTCACGCTGT TACAAAGAAC 
ACTAGGGAGT CTACACAGTA TAAACAGATG ATGCAAACTC TGAAGCAGAC CATGATGATG 
GTTGTGGAGT CACTAATCAA TAAATTTGAG GAAGATCAGA TGAAAAGAGA GGAAATGGAC 
CGGAAGATCC AGCACCAGCA GTCAATCAGT CAATACGCTG ACAACTGCTC TGACAGTGAC 
TCTTCATTCA ATCAGAGCTA TGCATTTATT AAACACGAAC AACTGCAAGT CATCGCTGAG 
AAGCTTGACC CTGGTAGACC GCGAGAGGTT CGCTGGGAAG CTTTGCAGTC TCTCTGCCAT 
GCCCCTCCTT CTGATGTTCT GAGCTGTGAA AGCTGGACTG GTCTTCGCAG AAACCTCTCC 
ACAGCACTCA CTGACCCAGA CCCTGAGCTC AGCGATAAAG TTCTTCAGTT TTTTGCAAAA 
ACATTTTCAT CCTCTCCATT AAATGTGACT AAGGATATCT ACGCTAGCAT AGCAAAAACC 
TTGGAGGCTC ATTTCCTTTA CCACAAGTTG AGTTTTCCCA GTGGCACTAC TAGTATTGAT 
GCAAACAGAC CAGATATGGC CCGTCTCCTC AAACAAATGC GATTAATGAA TGACTTTCAA 
AAGGAAGTGA CTACGTTTTG GATACGGCAT CCTGAGAAGT ACATGGAAGA AATCATTGAA 
AACACCCTGT CTCTTCTCGC TCTGCATTCT GAACAGGGCA TGAGCAGTCC CGGCTCAGAG 
AAATCCCTAG AGCCTATTCA CTTAATCTCT CTGCTGGATA TCAAAGCCAC CTGGTTTAAG 
AAATGGATGC ATGGTTACTA TAGCAGAACT GTTGTCTTAA GACTTCTGGA GAGGAAATAC 
AAGTCTTTGA TTGTTAATGC TCTGCAGCAG TGCATTTATT ACTTTGATTC CTGTGATGCA 
TTAAGTGAAG AGACCTTGGA AATGATCCAC AGCCTGGAAC ATCAGCAGAT TGGCCCCGCT 
GGAAGAACTC TCTACACATC TAAAGAGCTG GAGTTTGTTT ACTTCGTTCA TTCGCTGTGT 
GTTCTCGGGA GGCTTGTGAT GTACACTAAT GGAAGGAAAT TTTTTCCTAT TAAAGTGAAG 
AAGCGAAGAG ATCCGGTGAC TTTGACCGAC TTAGTCGTGA TTCTGATCAA TATAATGTAC 
CAACACCCAA AACCATCCTG CGGTGAGACG GCACTTGCAG ACACCATGTC CCCCACTAAT 
CTTGTGATGG AGGTGTTAAG GACCCTGTGT GACCGCACTG AGTGTGCTGT GGAGTGTCTT 
TACCAAATCC CTGTTATTGA GACTCTGCTG GCTCCTCTTA TCACTCTGCT CACAGGAAAG 
CTGCCAAAAT TAAATTCCAT GGAAAGTGCC CTGACTCACA CAGCAGACAC GCTGGCGAGG 
ATTGCTACCA CAGAACGAGG TTTATCACTC CTACTTTATG ACAGAAATCT CGTCTCGGCC 
GAGGGTGAAA GCATTTCAGC AGCACATCTA ATAGTGCAGT TTACCCAGAA GCTGTTGGCA 
AAGGAGCTGC AAGTGGAGAA TTCTTCTAGC ATGAGCGGGG CCTTCATCTT CGTCTGTCGG 
CAGATGTACA ACACCTGCGA GGGCCTGCAA GTTCTGCGGC CATACAGCCT GCATGAGTGC 
ATTGCTCAAG CCTGGAGAAC GACAAGCTCA ATGTCGGAAA GAGTTCCCAC TCCTGTTCCT 
GGAGCTCCCC CAGCACTGGC GTCCCAGGAG CTGCAGAGCG TGGTGGCCTG GGAAGAGATG 
CTGTTAGACA ACCTGTTGAA TTTCGCAGCC ACACCAAAAG GACTTTTGCT TCTTCAACAG 
ACCGGGGCCA TTAATGAGTG TGTCACTTAC ATGTTTTCAA GATTCACCAA GAAACTGCAG 
GTCAGCAGAT GTGAGAAGTT TGGTTATGGT GTTATGGTGA CACAAGTGGC CACTACAGCC 
CCTGGAATTG TGGCCTTGCA AAGCTCAGGA TTTGTCCAGA CAATTGTGGT GGAGCTGTGG 
TCAGCCTTGG AATGTGGACG AGAGGATGTA AGGGTGGTCC ATCCAAAATC TACTCCAATG 
GACCCAATTG ACAGGAGCTG CCTCAAGTCC TTCCTGTCTC TAGTGAATCT GCTTTCTTCA 
CCTCACGCTG TGTGGGAGCT TCTGGGACAT CGAGCACTTC CTAACAAGAC GGAGTACAAT 
CTGAGAGAGA TGGCCACATC CGTTTTAGAC CTGATGGACC GTCTCATCAT TATTAACTCT 
GACGCGAAGA TTCACTCTCT TTTCAACTAT GAACAGTCCC ACACGTTTGG CCTGAGACTC 
CTGAGTGTGC TTTGCTGTAA CCTGGATTCT TTTCTGCTGC TGGAGTCTCA GTATAAACTT 
ACTGACTTGC TGCTTCAGGG TCAGAGGGAC AATGTCACTG AGCCACCATC AGGTGAAGGG 
GAATTCATCA TTGATGGACT GTCTGTGGAA AGAAACCATA TCCTAGTTCG AATCTGTGCA 
GTTGGCGGTC CATCTGAGAG AAAACTTCCC CCCAGAGCCT TGCAGAAAGG GAATGACCCT 
TACCCATGGC CAATGGTGTC CACTTATCCT CTGCCTAAGT ATTATATATT CGACGTGCCC 
AAGATTCTCC GCACTAAACA GGAGTCAGAA ATTGGTGCTT TCCTCACCTC ATCTAAGGAA 
ACAGAGAGAG ATGCTAGCTG GATGGATGAC TGCAGGAGAC AGTTCTGCAA GATTATGGCG 
ACAAAGTCAA ACACTCTCAC AGGATACGTG CTTGCCGATC TGTTGGAGAT GGTCGTTCTG 
CATCTCTCGT CTTCATCCAA TGACTGCTTC TTTCCGCCTG CAGAATATAA AGTGGTGGAT 
AACAGTGTAA AGACTCGAAG CCTGTCATCT GTGGAGCAGC TAGGTGTGGA AATCTCACTC 
AGGTATGGCA AGTTTCTGAA GCTGCTGAGA GAAGATTCAG AGCAGGATCT GTGTCTTCTG 
CTGAAACACT GTCAGGAGTT TCTCTCTCAG CAACGTGTCA AGGTCACCTC TGAGCTCTCC 
CATTTTCAAG GTGGTTATCC TGGTCACGAT TGGTTTGCTT CTACAGTATT TCTGCTCATG 
CACGGTGATG TGGGCCGCTC TCTGAGTTTG CTCTTGCGTT TCTCCCGTCT CCTGCCGTCT 
GCCTTCCTGT GGCCTCCGAG ACTGCACAGC TCTGTCCATC TCCCTATAGA GATTGCTCAG 
TCGGCTATTC ATCCAATCTA CTCCTGCACC GCTCATTATG TAGAGATGTT GCTCAAGGCT 
GAGGTTCCTC TGGTTTTTTC TGCCTTCAGA ATGTCTGGCT TCACTCCCTC TCAGATCTGT 
GTTCAGTGGC TCGGCCAGTG CTTCTGGAAC TACCTGGACT GGCCTGAGAT CTGTCAGTAT 
GTGACCACCT GCGTCATTAT GGGACCCGAT TACCAGGTGT ACATGTGCGT CTCTGCTCTC 
CGTCACCTAC AGCAGGACAT CCTACAGCAC ACACAGACAC AAGACCTCCA GGTCTTCCTC 
AAGGAGGAAC CAATCCAAGG CTTCAGAGTG AGTAATTACC TGGAGTACAT GGAGGGGCTG 
GAGCGCAGCT ACAGATCCAT GGTGCTCTCG GACATGAGGA GCATCTTACC GAGGAGCAGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction.
Developmental cell18(2)237-47(2010 Feb)
Ko HW,Norman RX,Tran J,Fuller KP,Fukuda M,Eggenschwiler JT

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tbc1d32 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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