MYO5A cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYO5A
Entrez Gene ID	396237
Full Name	myosin VA
Synonyms	GS1,MYH12,MYO5,MYR12,myosin-12,myoxin
General protein information	Preferred Names myosin VA Names unconventional myosin-Va dilute myosin heavy chain, non-muscle myosin 5A myosin I heavy chain isoform myosin VA (heavy chain 12, myosin) myosin VA (heavy chain 12, myoxin) myosin VA (heavy polypeptide 12, myoxin) myosin heavy chain P190 myosin, heavy polypeptide kinase myosin-V myosin-Va
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205300.1	NP_990631.1	unconventional myosin-Va

Pathways from BioSystems

Homologies

	MYO5A	XP_001084476.1
Canis lupus familiaris (dog)	MYO5A	XP_005638745.1
Mus musculus (house mouse)	Myo5a	NP_034994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006479	XP_316519.4
Kluyveromyces lactis	KLLA0A03905g	XP_451171.1
Magnaporthe oryzae (rice blast fungus)	MGG_16213	XP_003709659.1
Arabidopsis thaliana (thale cress)	XIC	NP_172349.2
Oryza sativa (rice)	Os06g0488200	NP_001057670.1
Arabidopsis thaliana (thale cress)	XIE	NP_175858.1
Bos taurus (cattle)	MYO5A	XP_005211933.1
Rattus norvegicus (Norway rat)	Myo5a	NP_071514.1
Gallus gallus (chicken)	MYO5A	NP_990631.1
Neurospora crassa	NCU11354	XP_001728528.1
Xenopus tropicalis (tropical clawed frog)	myo5a	XP_004912752.1
Pan troglodytes (chimpanzee)	MYO5A	XP_510412.2
Danio rerio (zebrafish)	myo5aa	XP_005173731.1
Drosophila melanogaster (fruit fly)	didum	NP_724569.1
Caenorhabditis elegans (roundworm)	hum-2	NP_001023886.1
Saccharomyces cerevisiae (baker's yeast)	MYO2	NP_014971.1
Homo sapiens (human)	MYO5A	NP_000250.3
Eremothecium gossypii	AGOS_ADR354W	NP_984450.1

Gene Ontology
Bibliography

Related articles in PubMed

Tuning myosin-driven sorting on cellular actin networks.
Hariadi RF, Sommese RF, Sivaramakrishnan S
eLife4(2015 Mar)

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons.
Kanno TY, Espreafico EM, Yan CY
Cell biology international38(3)388-94(2014 Mar)

Switch II mutants reveal coupling between the nucleotide- and actin-binding regions in myosin V.
Trivedi DV, David C, Jacobs DJ, Yengo CM
Biophysical journal102(11)2545-55(2012 Jun)

EPR spectra and molecular dynamics agree that the nucleotide pocket of myosin V is closed and that it opens on binding actin.
Purcell TJ, Naber N, Sutton S, Cooke R, Pate E
Journal of molecular biology411(1)16-26(2011 Aug)

Simultaneous observation of tail and head movements of myosin V during processive motion.
Lu H, Kennedy GG, Warshaw DM, Trybus KM
The Journal of biological chemistry285(53)42068-74(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that cells direct the movement of vesicles around a cell by altering the relative number of myosin Va from Gallus gallus and myosin VI from Sus scrofa.
Title: Tuning myosin-driven sorting on cellular actin networks.

Myosin Va can participate in embryonic dorsal root ganglia neuritogenesis
Title: Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons.

In the presence of ADP and actin, both switch II mutants disrupt the formation of a closed nucleotide-binding pocket actomyosin.ADP state.
Title: Switch II mutants reveal coupling between the nucleotide- and actin-binding regions in myosin V.

The myosin V*adenosine diphosphate structure shows an open nucleotide site and has been proposed to be the A* myosin V* adenosine diphosphate state at the end of the working powerstroke.
Title: EPR spectra and molecular dynamics agree that the nucleotide pocket of myosin V is closed and that it opens on binding actin.

analysis of tail and head movements of myosin V during processive motion
Title: Simultaneous observation of tail and head movements of myosin V during processive motion.

The following MYO5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYO5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa26886	NM_205300.1 Latest version!	Gallus gallus myosin VA (MYO5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYO5A ( NM_205300.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990631.1 Gallus gallus myosin VA (MYO5A), mRNA.

Price & Availability↑

CloneID	OGa26886
Clone ID Related Accession (Same CDS sequence)	NM_205300.1
Accession Version	NM_205300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	unconventional myosin-Va
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X67251.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X67251.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCCGCCT CCGAGCTGTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA CCCAGAGGAG 
GTCTGGAAGT CAGCAGAACT TCTTAAAGAT TATAAACCTG GAGATAAAGT TTTGCAGCTT 
CGACTTGAAG AGGGCAAGGA CCTAGAATAT TGCCTCGACC CCAAGACTAA GGAACTCCCC 
CCCTTGCGAA ACCCTGACAT CCTTGTTGGT GAAAATGACC TCACTGCGCT CAGTTATCTC 
CATGAACCTG CTGTGCTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTCATTTAT 
ACCTATTGTG GTATCGTCTT AGTGGCAATA AACCCTTATG AACAGCTGCC TATCTATGGC 
GAAGATATCA TCAATGCGTA CAGTGGCCAA AATATGGGGG ATATGGATCC ACATATCTTT 
GCAGTGGCAG AAGAGGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT 
GTAAGTGGAG AATCTGGGGC AGGAAAGACG GTTTCTGCCA AGTATGCCAT GAGGTACTTT 
GCCACGGTCA GTGGATCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCTTCAAAC 
CCCATAATGG AGTCCATTGG AAATGCCAAA ACAACGAGGA ATGACAACAG CAGTCGCTTT 
GGGAAATACA TTGAAATTGG TTTTGACAAG AGGTATCGAA TCATTGGTGC TAACATGAGA 
ACTTATCTCT TGGAGAAATC AAGAGTGGTA TTTCAGGCAG AAGAGGAGAG GAATTACCAC 
ATCTTTTACC AACTCTGTGC CTCTGCAGCA TTACCTGAAT TTAAAACTCT ACGATTAGGG 
AATGCAAATT ACTTTCATTA TACAAAGCAA GGTGGAAGCC CTGTGATTGA TGGCATTGAT 
GATGCTAAGG AAATGGTAAA CACCAGACAA GCCTGCACTT TGCTAGGGAT TAGTGATTCC 
TACCAGATGG GAATTTTCCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT 
GCATCTCGGG ATTCTGACAG CTGCGCCATT CCTCCCAAAC ATGATCCCCT CACCATCTTC 
TGTGACCTCA TGGGCGTGGA CTATGAAGAG ATGGCTCACT GGCTTTGCCA TAGGAAGCTC 
GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA 
GATGCACTTG CCAAACATAT CTATGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC 
AAAGCCCTTC ATTCTACTGT AAAGCAACAT TCTTTCATTG GAGTACTGGA CATTTATGGA 
TTTGAGACAT TTGAAATCAA CAGCTTCGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTGCAGCAGC AGTTCAATAT GCATGTGTTT AAGCTGGAAC AAGAAGAATA CATGAAAGAA 
CAAATACCAT GGACCTTGAT TGATTTCTAT GACAATCAGC CTTGCATCAA CCTCATAGAA 
GCCAAAATGG GAGTTCTGGA TCTGTTAGAT GAGGAATGCA AGATGCCAAA AGGCTCGGAT 
GACACTTGGG CCCAAAAACT GTACAATACT CATTTGAATA AATGTGCCCT CTTTGAAAAA 
CCACGTTTAT CCAATAAGGC TTTTATCATC AAACACTTTG CTGACAAGGT GGAATATCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAGGAT ACAGTTTATG AAGAGCAAAT TAAGGTCCTA 
AAATCAAGTA AGAAGTTTAA GCTGCTACCA GAATTATTCC AGGATGAGGA GAAGGCCATC 
AGTCCCACGT CAGCCACCCC TTCAGGCCGT GTGCCATTGT CTCGAACTCC TGTAAAACCA 
GCCAAGGCCA GGCCAGGTCA AACTAGCAAG GAGCATAAGA AAACTGTGGG GCATCAGTTT 
CGAAACTCTC TTCATCTGCT GATGGAAACC CTGAACGCCA CAACTCCACA CTATGTGCGC 
TGTATTAAGC CTAACGACTT CAAGTTTCCA TTCACATTTG ACGAAAAGCG AGCAGTGCAG 
CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG TTTCCCCTCC 
AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TCATGAAGCA GAAAGATGTC 
CTTAGTGACC GAAAACAGAC ATGTAAAAAT GTCCTGGAGA AGCTGATTCT GGACAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTTGAA 
AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC 
TGGCTGATGA GAAAGAAGTA CATGCGTATG AGGAGGGCTG CCATCACCAT TCAGAGATAT 
GTCAGAGGGC ACCAAGCACG ATGCTATGCC ACATTCCTGC GGAGAACACG GGCTGCCATC 
ATTATTCAGA AGTTCCAGCG TATGTATGTG GTCCGCAAAA GATACCAGTG CATGCGAGAT 
GCTACTATTG CTCTTCAAGC TCTCTTAAGA GGTTACCTGG TCAGGAACAA GTACCAAATG 
ATGCTTCGAG AGCACAAGTC TATTATTATT CAGAAACATG TAAGAGGCTG GCTGGCTCGA 
GTGCACTATC ATAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG 
ATGGCCAAGA GGGAGCTGAA GAAGCTGAAG ATAGAGGCTC GGTCTGTGGA ACGCTACAAG 
AAGCTTCACA TTGGCTTGGA GAACAAGATC ATGCAGCTGC AGCGGAAAAT TGATGAACAG 
AACAAAGAGT ACAAATCTCT GCTGGAGAAG ATGAATAACC TGGAGATCAC ATACAGTACA 
GAGACAGAGA AGCTTCGGAG TGATGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG 
AATGCAACCA ACCGTGTTCT CAGCCTTCAG GAAGAGATTG CCAAGCTCCG GAAGGAGCTG 
CACCAGACTC AGACTGAGAA GAAGACAATT GAGGAATGGG CAGACAAATA CAAACATGAA 
ACTGAGCAGC TGGTGTCGGA ACTGAAAGAG CAGAACACAT TACTGAAAAC AGAAAAGGAG 
GAGCTGAACC GCCGTATCCA TGACCAAGCA AAGGAGATAA CAGAGACAAT GGAGAAGAAG 
CTAGTGGAGG AAACAAAGCA GTTGGAGCTA GACCTGAACG ATGAGAGGTT ACGGTATCAG 
AACCTGCTGA ATGAGTTCAG CCGCTTGGAG GAGCGGTATG ATGATCTCAA GGATGAAATG 
AACTTAATGG TGAGCATCCC CAAGCCTGGA CACAAAAGAA CGGATTCAAC TCACAGTAGC 
AATGAATCTG AATATACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCACTGAGG 
ATGGAGGAGC CAAGTGAGAA AAAGGCACCA TTGGACATGT CTCTGTTCCT CAAGCTGCAG 
AAACGGGTTA CAGAGCTGGA GCAAGAAAAG CAATCCCTGC AGGATGAACT GGACAGAAAG 
GAAGAACAGG CTCTTCGTGC CAAAGCTAAG GAGGAGGAAA GGCCTCCAAT AAGAGGTGCA 
GAGTTGGAGT ATGAGTCGCT CAAGCGTCAA GAGCTTGAAT CTGAGAATAA AAAACTGAAG 
AATGAGTTGA ACGAGCTGCA GAAGGCCCTC ACAGAAACAC GAGCTCCAGA GGTAACTGCT 
CCTGGTGCTC CAGCATACAG AGTCCTCCTG GATCAGCTGA CTTCAGTAAG CGAAGAACTG 
GAAGTACGCA AGGAAGAAGT GCTTATCCTG AGGTCTCAGT TGGTTAGCCA GAAAGAGGCT 
ATTCAACCCA AGGAGGACAA GAACACCATG ACAGATTCTA CAATCCTCTT GGAGGATGTG 
CAGAAGATGA AAGACAAAGG GGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACCAAC 
AGGCTGCTGG AGTCTCAGCT GCAGTCGCAG AAGAAGAGCC ACGAGAATGA GCTGGAGTCA 
CTGCGAGGTG AGATCCAAAG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCTTGGCA 
CAGAATCTGC AGCTGCCCCC GGAGGCCCGC ATTGAAGCCA GTCTGCAGCA TGAGATCACC 
CGGCTGACAA ACGAGAACTT GGATTTAATG GAACAACTTG AAAAGCAAGA CAAAACTGTT 
CGCAAATTAA AGAAGCAATT GAAGGTATTT GCTAAGAAGA TTGGTGAACT TGAAGTGGGT 
CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT 
CCACGGAAGG AAAAGGACTT CCAGGGGATG TTGGAGTATA AGAAAGAGGA TGAACAAAAG 
CTAGTCAAGA ATCTTATATT AGAGCTGAAA CCACGCGGGG TAGCAGTCAA CCTGATTCCA 
GGACTACCAG CATATATTTT GTTCATGTGT GTACGCCACG CAGATTACCT TAACGATGAC 
CAGAAAGTGC GTTCATTGTT GACCTCCACT ATCAATGGCA TCAAAAAAGT GCTGAAGAAA 
AGAGGTGATG ACTTCGAAAC GGTGTCTTTC TGGCTGTCCA ACACCTGCCG GTTTTTGCAC 
TGTTTGAAGC AGTACAGCGG AGAAGAGGGG TTTATGAAGC ATAATACACC TCGTCAGAAT 
GAGCACTGCC TCACTAATTT TGACTTAGCT GAATACAGAC AAGTCCTGAG TGACTTGGCT 
ATTCAGATCT ACCAGCAACT GGTCCGCGTG TTGGAGAACA TTCTGCAACC CATGATTGTT 
TCTGGAATGC TGGAGCACGA GACTATCCAG GGTGTCTCAG GGGTGAAACC AACAGGGCTG 
CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGA 
CAGTTGAACT CTTTCCATTC CGTGATGTGT CAGCATGGAA TGGATCCAGA GCTGATCAAA 
CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CACTTAATAA TCTTCTCCTG 
CGCAAGGACA TGTGTTCATG GAGCAAAGGA ATGCAGATAA GGTACAATGT GAGTCAACTT 
GAAGAATGGC TACGTGATAA AAATCTAATG AACAGTGGGG CCAAAGAAAC ACTGGAGCCC 
CTCATACAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC 
ATTTGTTCAA TGTGCAATGC ACTGACTACT GCCCAGATTG TGAAAGTACT GAATTTGTAT 
ACTCCAGTTA ATGAATTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGCTG 
CGTCTGCGAG ACAGGAAGGA CTCTCCTCAA CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTTACTTTTC CATTTAATCC ATCCTCCCTG GCATTAGAAA CCATCCAGAT CCCAGCCAGT 
TTGGGGCTGG GTTTCATATC ACGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990631.1
CDS	100..5589
Translation	MAASELYTKYARVWIPDPEEVWKSAELLKDYKPGDKVLQLRLEEGKDLEYCLDPKTKELPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIFAVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLASNPIMESIGNAKTTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAALPEFKTLRLGNANYFHYTKQGGSPVIDGIDDAKEMVNTRQACTLLGISDSYQMGIFRILAGILHLGNVEFASRDSDSCAIPPKHDPLTIFCDLMGVDYEEMAHWLCHRKLATATETYIKPISKLHAINARDALAKHIYANLFNWIVDHVNKALHSTVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFYDNQPCINLIEAKMGVLDLLDEECKMPKGSDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIKHFADKVEYQCEGFLEKNKDTVYEEQIKVLKSSKKFKLLPELFQDEEKAISPTSATPSGRVPLSRTPVKPAKARPGQTSKEHKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKFPFTFDEKRAVQQLRACGVLETIRISAAGFPSRWTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKIRADKLRAACIRIQKTIRGWLMRKKYMRMRRAAITIQRYVRGHQARCYATFLRRTRAAIIIQKFQRMYVVRKRYQCMRDATIALQALLRGYLVRNKYQMMLREHKSIIIQKHVRGWLARVHYHRTLKAIVYLQCCYRRMMAKRELKKLKIEARSVERYKKLHIGLENKIMQLQRKIDEQNKEYKSLLEKMNNLEITYSTETEKLRSDVERLRMSEEEAKNATNRVLSLQEEIAKLRKELHQTQTEKKTIEEWADKYKHETEQLVSELKEQNTLLKTEKEELNRRIHDQAKEITETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKDEMNLMVSIPKPGHKRTDSTHSSNESEYTFSSEITEAEDLPLRMEEPSEKKAPLDMSLFLKLQKRVTELEQEKQSLQDELDRKEEQALRAKAKEEERPPIRGAELEYESLKRQELESENKKLKNELNELQKALTETRAPEVTAPGAPAYRVLLDQLTSVSEELEVRKEEVLILRSQLVSQKEAIQPKEDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRLLESQLQSQKKSHENELESLRGEIQSLKEENNRQQQLLAQNLQLPPEARIEASLQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKEKDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINGIKKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTPRQNEHCLTNFDLAEYRQVLSDLAIQIYQQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSIIRQLNSFHSVMCQHGMDPELIKQVVKQMFYIIGAVTLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQAAQLLQVKKKTDEDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVLVSFIRTIQLRLRDRKDSPQLLMDAKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV

Target ORF information:

RefSeq Version	NM_205300.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus myosin VA (MYO5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205300.1

ORF Insert Sequence:

ATGGCCGCCT CCGAGCTGTA CACCAAGTAT GCCAGGGTTT GGATCCCTGA CCCAGAGGAG 
GTCTGGAAGT CAGCAGAACT TCTTAAAGAT TATAAACCTG GAGATAAAGT TTTGCAGCTT 
CGACTTGAAG AGGGCAAGGA CCTAGAATAT TGCCTCGACC CCAAGACTAA GGAACTCCCC 
CCCTTGCGAA ACCCTGACAT CCTTGTTGGT GAAAATGACC TCACTGCGCT CAGTTATCTC 
CATGAACCTG CTGTGCTACA CAACCTCAAA GTTCGATTTA TAGACTCTAA ACTCATTTAT 
ACCTATTGTG GTATCGTCTT AGTGGCAATA AACCCTTATG AACAGCTGCC TATCTATGGC 
GAAGATATCA TCAATGCGTA CAGTGGCCAA AATATGGGGG ATATGGATCC ACATATCTTT 
GCAGTGGCAG AAGAGGCATA CAAGCAGATG GCCAGAGATG AGCGAAATCA GTCAATCATT 
GTAAGTGGAG AATCTGGGGC AGGAAAGACG GTTTCTGCCA AGTATGCCAT GAGGTACTTT 
GCCACGGTCA GTGGATCTGC CAGTGAAGCC AATGTTGAGG AGAAAGTCTT GGCTTCAAAC 
CCCATAATGG AGTCCATTGG AAATGCCAAA ACAACGAGGA ATGACAACAG CAGTCGCTTT 
GGGAAATACA TTGAAATTGG TTTTGACAAG AGGTATCGAA TCATTGGTGC TAACATGAGA 
ACTTATCTCT TGGAGAAATC AAGAGTGGTA TTTCAGGCAG AAGAGGAGAG GAATTACCAC 
ATCTTTTACC AACTCTGTGC CTCTGCAGCA TTACCTGAAT TTAAAACTCT ACGATTAGGG 
AATGCAAATT ACTTTCATTA TACAAAGCAA GGTGGAAGCC CTGTGATTGA TGGCATTGAT 
GATGCTAAGG AAATGGTAAA CACCAGACAA GCCTGCACTT TGCTAGGGAT TAGTGATTCC 
TACCAGATGG GAATTTTCCG AATCCTTGCT GGCATCCTTC ACTTGGGCAA CGTGGAGTTT 
GCATCTCGGG ATTCTGACAG CTGCGCCATT CCTCCCAAAC ATGATCCCCT CACCATCTTC 
TGTGACCTCA TGGGCGTGGA CTATGAAGAG ATGGCTCACT GGCTTTGCCA TAGGAAGCTC 
GCAACTGCCA CTGAAACCTA CATCAAGCCA ATTTCTAAAC TTCATGCCAT CAATGCCAGA 
GATGCACTTG CCAAACATAT CTATGCTAAT CTCTTTAACT GGATTGTAGA TCATGTGAAC 
AAAGCCCTTC ATTCTACTGT AAAGCAACAT TCTTTCATTG GAGTACTGGA CATTTATGGA 
TTTGAGACAT TTGAAATCAA CAGCTTCGAA CAGTTCTGTA TCAACTATGC CAATGAGAAA 
CTGCAGCAGC AGTTCAATAT GCATGTGTTT AAGCTGGAAC AAGAAGAATA CATGAAAGAA 
CAAATACCAT GGACCTTGAT TGATTTCTAT GACAATCAGC CTTGCATCAA CCTCATAGAA 
GCCAAAATGG GAGTTCTGGA TCTGTTAGAT GAGGAATGCA AGATGCCAAA AGGCTCGGAT 
GACACTTGGG CCCAAAAACT GTACAATACT CATTTGAATA AATGTGCCCT CTTTGAAAAA 
CCACGTTTAT CCAATAAGGC TTTTATCATC AAACACTTTG CTGACAAGGT GGAATATCAG 
TGTGAAGGCT TTCTGGAAAA GAATAAGGAT ACAGTTTATG AAGAGCAAAT TAAGGTCCTA 
AAATCAAGTA AGAAGTTTAA GCTGCTACCA GAATTATTCC AGGATGAGGA GAAGGCCATC 
AGTCCCACGT CAGCCACCCC TTCAGGCCGT GTGCCATTGT CTCGAACTCC TGTAAAACCA 
GCCAAGGCCA GGCCAGGTCA AACTAGCAAG GAGCATAAGA AAACTGTGGG GCATCAGTTT 
CGAAACTCTC TTCATCTGCT GATGGAAACC CTGAACGCCA CAACTCCACA CTATGTGCGC 
TGTATTAAGC CTAACGACTT CAAGTTTCCA TTCACATTTG ACGAAAAGCG AGCAGTGCAG 
CAGCTGAGAG CTTGTGGTGT CCTGGAGACC ATCCGAATCA GTGCAGCTGG TTTCCCCTCC 
AGGTGGACGT ACCAAGAGTT CTTCAGCCGT TACCGTGTTC TCATGAAGCA GAAAGATGTC 
CTTAGTGACC GAAAACAGAC ATGTAAAAAT GTCCTGGAGA AGCTGATTCT GGACAAGGAT 
AAGTACCAGT TTGGTAAGAC AAAAATATTT TTCCGGGCTG GTCAAGTAGC CTATCTTGAA 
AAAATAAGGG CAGATAAGTT GAGAGCTGCC TGTATCCGCA TCCAAAAGAC AATCCGAGGC 
TGGCTGATGA GAAAGAAGTA CATGCGTATG AGGAGGGCTG CCATCACCAT TCAGAGATAT 
GTCAGAGGGC ACCAAGCACG ATGCTATGCC ACATTCCTGC GGAGAACACG GGCTGCCATC 
ATTATTCAGA AGTTCCAGCG TATGTATGTG GTCCGCAAAA GATACCAGTG CATGCGAGAT 
GCTACTATTG CTCTTCAAGC TCTCTTAAGA GGTTACCTGG TCAGGAACAA GTACCAAATG 
ATGCTTCGAG AGCACAAGTC TATTATTATT CAGAAACATG TAAGAGGCTG GCTGGCTCGA 
GTGCACTATC ATAGGACCTT GAAGGCAATT GTTTACTTGC AATGCTGTTA CCGGCGCATG 
ATGGCCAAGA GGGAGCTGAA GAAGCTGAAG ATAGAGGCTC GGTCTGTGGA ACGCTACAAG 
AAGCTTCACA TTGGCTTGGA GAACAAGATC ATGCAGCTGC AGCGGAAAAT TGATGAACAG 
AACAAAGAGT ACAAATCTCT GCTGGAGAAG ATGAATAACC TGGAGATCAC ATACAGTACA 
GAGACAGAGA AGCTTCGGAG TGATGTGGAG AGGCTTCGGA TGAGTGAGGA GGAGGCTAAG 
AATGCAACCA ACCGTGTTCT CAGCCTTCAG GAAGAGATTG CCAAGCTCCG GAAGGAGCTG 
CACCAGACTC AGACTGAGAA GAAGACAATT GAGGAATGGG CAGACAAATA CAAACATGAA 
ACTGAGCAGC TGGTGTCGGA ACTGAAAGAG CAGAACACAT TACTGAAAAC AGAAAAGGAG 
GAGCTGAACC GCCGTATCCA TGACCAAGCA AAGGAGATAA CAGAGACAAT GGAGAAGAAG 
CTAGTGGAGG AAACAAAGCA GTTGGAGCTA GACCTGAACG ATGAGAGGTT ACGGTATCAG 
AACCTGCTGA ATGAGTTCAG CCGCTTGGAG GAGCGGTATG ATGATCTCAA GGATGAAATG 
AACTTAATGG TGAGCATCCC CAAGCCTGGA CACAAAAGAA CGGATTCAAC TCACAGTAGC 
AATGAATCTG AATATACTTT TAGCTCTGAG ATCACAGAAG CAGAAGACTT ACCACTGAGG 
ATGGAGGAGC CAAGTGAGAA AAAGGCACCA TTGGACATGT CTCTGTTCCT CAAGCTGCAG 
AAACGGGTTA CAGAGCTGGA GCAAGAAAAG CAATCCCTGC AGGATGAACT GGACAGAAAG 
GAAGAACAGG CTCTTCGTGC CAAAGCTAAG GAGGAGGAAA GGCCTCCAAT AAGAGGTGCA 
GAGTTGGAGT ATGAGTCGCT CAAGCGTCAA GAGCTTGAAT CTGAGAATAA AAAACTGAAG 
AATGAGTTGA ACGAGCTGCA GAAGGCCCTC ACAGAAACAC GAGCTCCAGA GGTAACTGCT 
CCTGGTGCTC CAGCATACAG AGTCCTCCTG GATCAGCTGA CTTCAGTAAG CGAAGAACTG 
GAAGTACGCA AGGAAGAAGT GCTTATCCTG AGGTCTCAGT TGGTTAGCCA GAAAGAGGCT 
ATTCAACCCA AGGAGGACAA GAACACCATG ACAGATTCTA CAATCCTCTT GGAGGATGTG 
CAGAAGATGA AAGACAAAGG GGAAATAGCA CAGGCGTATA TTGGACTGAA GGAAACCAAC 
AGGCTGCTGG AGTCTCAGCT GCAGTCGCAG AAGAAGAGCC ACGAGAATGA GCTGGAGTCA 
CTGCGAGGTG AGATCCAAAG TCTGAAGGAA GAAAACAATC GCCAGCAGCA GCTCTTGGCA 
CAGAATCTGC AGCTGCCCCC GGAGGCCCGC ATTGAAGCCA GTCTGCAGCA TGAGATCACC 
CGGCTGACAA ACGAGAACTT GGATTTAATG GAACAACTTG AAAAGCAAGA CAAAACTGTT 
CGCAAATTAA AGAAGCAATT GAAGGTATTT GCTAAGAAGA TTGGTGAACT TGAAGTGGGT 
CAGATGGAGA ACATATCACC TGGACAAATC ATTGATGAAC CTATTCGTCC AGTTAACATT 
CCACGGAAGG AAAAGGACTT CCAGGGGATG TTGGAGTATA AGAAAGAGGA TGAACAAAAG 
CTAGTCAAGA ATCTTATATT AGAGCTGAAA CCACGCGGGG TAGCAGTCAA CCTGATTCCA 
GGACTACCAG CATATATTTT GTTCATGTGT GTACGCCACG CAGATTACCT TAACGATGAC 
CAGAAAGTGC GTTCATTGTT GACCTCCACT ATCAATGGCA TCAAAAAAGT GCTGAAGAAA 
AGAGGTGATG ACTTCGAAAC GGTGTCTTTC TGGCTGTCCA ACACCTGCCG GTTTTTGCAC 
TGTTTGAAGC AGTACAGCGG AGAAGAGGGG TTTATGAAGC ATAATACACC TCGTCAGAAT 
GAGCACTGCC TCACTAATTT TGACTTAGCT GAATACAGAC AAGTCCTGAG TGACTTGGCT 
ATTCAGATCT ACCAGCAACT GGTCCGCGTG TTGGAGAACA TTCTGCAACC CATGATTGTT 
TCTGGAATGC TGGAGCACGA GACTATCCAG GGTGTCTCAG GGGTGAAACC AACAGGGCTG 
CGGAAGAGAA CATCCAGCAT TGCTGATGAA GGAACCTACA CTTTGGACTC CATTATTCGA 
CAGTTGAACT CTTTCCATTC CGTGATGTGT CAGCATGGAA TGGATCCAGA GCTGATCAAA 
CAGGTTGTCA AGCAGATGTT CTACATCATT GGGGCTGTAA CACTTAATAA TCTTCTCCTG 
CGCAAGGACA TGTGTTCATG GAGCAAAGGA ATGCAGATAA GGTACAATGT GAGTCAACTT 
GAAGAATGGC TACGTGATAA AAATCTAATG AACAGTGGGG CCAAAGAAAC ACTGGAGCCC 
CTCATACAGG CTGCACAGTT GTTGCAAGTG AAAAAGAAAA CAGATGAAGA TGCAGAAGCC 
ATTTGTTCAA TGTGCAATGC ACTGACTACT GCCCAGATTG TGAAAGTACT GAATTTGTAT 
ACTCCAGTTA ATGAATTTGA AGAGAGAGTC TTGGTATCGT TTATACGTAC AATACAGCTG 
CGTCTGCGAG ACAGGAAGGA CTCTCCTCAA CTGCTCATGG ATGCTAAACA CATCTTTCCT 
GTTACTTTTC CATTTAATCC ATCCTCCCTG GCATTAGAAA CCATCCAGAT CCCAGCCAGT 
TTGGGGCTGG GTTTCATATC ACGTGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tuning myosin-driven sorting on cellular actin networks.
eLife4(2015 Mar)
Hariadi RF,Sommese RF,Sivaramakrishnan S

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons.
Cell biology international38(3)388-94(2014 Mar)
Kanno TY,Espreafico EM,Yan CY

Switch II mutants reveal coupling between the nucleotide- and actin-binding regions in myosin V.
Biophysical journal102(11)2545-55(2012 Jun)
Trivedi DV,David C,Jacobs DJ,Yengo CM

EPR spectra and molecular dynamics agree that the nucleotide pocket of myosin V is closed and that it opens on binding actin.
Journal of molecular biology411(1)16-26(2011 Aug)
Purcell TJ,Naber N,Sutton S,Cooke R,Pate E

Simultaneous observation of tail and head movements of myosin V during processive motion.
The Journal of biological chemistry285(53)42068-74(2010 Dec)
Lu H,Kennedy GG,Warshaw DM,Trybus KM

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MYO5A cDNA ORF clone, Gallus gallus(chicken)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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