mink1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mink1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mink1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa29569 XM_012953858.2
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OXa40993 XM_018089846.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa40994 XM_018089847.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa40997 XM_018089850.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa40998 XM_018089851.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa53687 XM_031898076.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53688 XM_031898077.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53689 XM_031898078.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53690 XM_031898079.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53691 XM_031898080.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53692 XM_018089850.2
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53693 XM_031898081.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa53694 XM_031898082.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa22550 NM_001079271.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa29571 XM_012953860.2
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa29574 XM_012953863.2
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa40995 XM_018089848.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OXa40996 XM_018089849.1
Latest version!
Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa29569
    Clone ID Related Accession (Same CDS sequence) XM_012953858.2
    Accession Version XM_012953858.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAGAT
    CACCTCCTGG TGAAGGACCG GCCCGAAGAT GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC
    TACTCTTCCT CCAGCGATGA ACTGGACAGC AGTGATGAAG AGGAAGAGGA GGGGGATGCT
    GAGAGACAGG AGCCCAACAG GGACAGCTCA GGAGCCAGTC GCAGCGGGCG AGACGCCGAT
    ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTGGAGG AGCTGATGGG GAGCACGTCT
    GACAGTTCCT ACGGCGACGG CACCATGGTG GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT
    CTCCTTCATG CTGACAGTAA CGGATATACC AACCTGCCCG ACGTGGTGCA ACCGAGCCAT
    TCTCCCACCG AGCCCAAGGG ACAGAGCACT TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT
    TACCAGTCTC GCGGGTTGGT GAAAGCCCCT CCCAAAACCT CATTTACAAT GTTTGTGGAC
    CTGGGACTGT ATCAGTCGTC GGGGGGAGGA GGAGACACCA TTCCGGTGGC AGCGGCGTAC
    GTGAGCGGAG ACCCCGCCCG ACTGGAGCAG CTGAAATACG AAGCCCGTAA GGGATCTGTC
    GTCAATGTAA ATCCGACCAA TACGAGGCCG CACAGCGACA CGCCGGAGAT CCGCAAGTAC
    AAGAAGAGAT TCAACTCGGA AATCCTTTGC GCCGCTCTCT GGGGTGTGAA CCTATTGGTC
    GGCACTGAGA ATGGACTGAT GCTACTGGAC CGCAGTGGGC AGGGCAAAGT ATACACCCTA
    ATTACCCGCC GGCGCTTCCA GCAGATGGAC GTCCTTGAGG GGCTCAACTT GCTCATTACT
    ATAAGTGGTA AGAGGAACAA GCTGCGGGTG TATTATCTCT CCTGGCTGCG CAACAAGATC
    CTTCATAACG ACCCCGAGGT GGAGAAGAAG CAGGGCTGGA GCACCGTGGG GGACATGGAC
    GGCTGCGTGC ATTACAAAGT CGTAAAATAC GAGCGAATCA AATTCCTCGT GATTGCACTG
    AAAAACTCCG TCGAGGTTTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT TATGGCTTTT
    AAGTCGTTTA CAGATATCCC CCACCGGCCC CAGCTGGTCG ACCTAACGGT GGAGGAAGGC
    CAGAGGCTCA AGGTCATCTA CGGTTCCTCG TCGGGATTCC ACGCCGTGGA TGTCGACTCC
    GGGAACAACT ATGATATTTA CATCCCTGTC CATATCCAGA GCCAGATTAA CCCCCACGCC
    ATCATCTTTC TGCCAAACAC GGACGGCATG GAGATGTTAC TGTGCTACGA GGACGAGGGG
    GTCTACGTCA ACACCTACGG GCGCATCATT AAAGACGTGG TGTTGCAGTG GGGAGAGATG
    CCCACGTCTG TCGCCTATAT CTGCTCCAAT CAGATCATGG GCTGGGGGGA GAAGGCCATA
    GAAATCCGCT CGGTGGAGAC GGGGCACCTG GACGGGGTCT TCATGCACAA GAGAGCTCAG
    CGGCTGAAGT TCCTGTGTGA GCGCAACGAC AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC
    GGGAGCAGCC AGGTTTACTT CATGACCCTG AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809312.1
    CDS1358..5254
    Translation

    Target ORF information:

    RefSeq Version XM_012953858.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953858.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAGAT
    CACCTCCTGG TGAAGGACCG GCCCGAAGAT GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC
    TACTCTTCCT CCAGCGATGA ACTGGACAGC AGTGATGAAG AGGAAGAGGA GGGGGATGCT
    GAGAGACAGG AGCCCAACAG GGACAGCTCA GGAGCCAGTC GCAGCGGGCG AGACGCCGAT
    ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTGGAGG AGCTGATGGG GAGCACGTCT
    GACAGTTCCT ACGGCGACGG CACCATGGTG GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT
    CTCCTTCATG CTGACAGTAA CGGATATACC AACCTGCCCG ACGTGGTGCA ACCGAGCCAT
    TCTCCCACCG AGCCCAAGGG ACAGAGCACT TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT
    TACCAGTCTC GCGGGTTGGT GAAAGCCCCT CCCAAAACCT CATTTACAAT GTTTGTGGAC
    CTGGGACTGT ATCAGTCGTC GGGGGGAGGA GGAGACACCA TTCCGGTGGC AGCGGCGTAC
    GTGAGCGGAG ACCCCGCCCG ACTGGAGCAG CTGAAATACG AAGCCCGTAA GGGATCTGTC
    GTCAATGTAA ATCCGACCAA TACGAGGCCG CACAGCGACA CGCCGGAGAT CCGCAAGTAC
    AAGAAGAGAT TCAACTCGGA AATCCTTTGC GCCGCTCTCT GGGGTGTGAA CCTATTGGTC
    GGCACTGAGA ATGGACTGAT GCTACTGGAC CGCAGTGGGC AGGGCAAAGT ATACACCCTA
    ATTACCCGCC GGCGCTTCCA GCAGATGGAC GTCCTTGAGG GGCTCAACTT GCTCATTACT
    ATAAGTGGTA AGAGGAACAA GCTGCGGGTG TATTATCTCT CCTGGCTGCG CAACAAGATC
    CTTCATAACG ACCCCGAGGT GGAGAAGAAG CAGGGCTGGA GCACCGTGGG GGACATGGAC
    GGCTGCGTGC ATTACAAAGT CGTAAAATAC GAGCGAATCA AATTCCTCGT GATTGCACTG
    AAAAACTCCG TCGAGGTTTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT TATGGCTTTT
    AAGTCGTTTA CAGATATCCC CCACCGGCCC CAGCTGGTCG ACCTAACGGT GGAGGAAGGC
    CAGAGGCTCA AGGTCATCTA CGGTTCCTCG TCGGGATTCC ACGCCGTGGA TGTCGACTCC
    GGGAACAACT ATGATATTTA CATCCCTGTC CATATCCAGA GCCAGATTAA CCCCCACGCC
    ATCATCTTTC TGCCAAACAC GGACGGCATG GAGATGTTAC TGTGCTACGA GGACGAGGGG
    GTCTACGTCA ACACCTACGG GCGCATCATT AAAGACGTGG TGTTGCAGTG GGGAGAGATG
    CCCACGTCTG TCGCCTATAT CTGCTCCAAT CAGATCATGG GCTGGGGGGA GAAGGCCATA
    GAAATCCGCT CGGTGGAGAC GGGGCACCTG GACGGGGTCT TCATGCACAA GAGAGCTCAG
    CGGCTGAAGT TCCTGTGTGA GCGCAACGAC AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC
    GGGAGCAGCC AGGTTTACTT CATGACCCTG AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40993
    Clone ID Related Accession (Same CDS sequence) XM_018089846.1
    Accession Version XM_018089846.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC CGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGAGAAGGC CATAGAAATC CGCTCGGTGG AGACGGGGCA CCTGGACGGG
    GTCTTCATGC ACAAGAGAGC TCAGCGGCTG AAGTTCCTGT GTGAGCGCAA CGACAAGGTG
    TTCTTTGCCT CGGTGCGTTC GGGCGGGAGC AGCCAGGTTT ACTTCATGAC CCTGAATCGC
    AGCTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945335.1
    CDS1358..5278
    Translation

    Target ORF information:

    RefSeq Version XM_018089846.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC CGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGAGAAGGC CATAGAAATC CGCTCGGTGG AGACGGGGCA CCTGGACGGG
    GTCTTCATGC ACAAGAGAGC TCAGCGGCTG AAGTTCCTGT GTGAGCGCAA CGACAAGGTG
    TTCTTTGCCT CGGTGCGTTC GGGCGGGAGC AGCCAGGTTT ACTTCATGAC CCTGAATCGC
    AGCTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40994
    Clone ID Related Accession (Same CDS sequence) XM_018089847.1
    Accession Version XM_018089847.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATATA TCAGGCACAA GTTAGAGGAA GAACAGAGGC AGTTAGAAAT CCTTCAGCAG
    CAGCTTCTGC AGGAGCAGGC ACTCTTACTG GAATACAAGC GGAAGCAGCT GGAGGAGCAG
    AGACAGTCAG AGAGACTGCA GAGACAGCTT CAGCAGGAAC ACGCTTATCT CAAGTCTCTG
    CAGCAGCAGC AACAGCAACA ACAGAAGCAG CAACAGCAGC AGCAGCAGGA TAAGAAGCCG
    CTGTACCATT ACAACCGGGG GGTAATGAAC CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG
    GTGGAGGAGC GTTCGAGGCT CAACAAGCAG AGTTCCCCCC TCTCCATTAC TAAGCTCAGC
    TCGGTGGAGT CTACTGGAAA CCCGTCGCAA ACCCCACCCT CTCAGCGGCC TCCAGAAATA
    CAGGAGCACA AGCCACTACG CATTACTTCT CAGAACCGTG CTGCTCTCAG GCCAGCTCCC
    CGGCCCAACT CCTTGCAGGA CCCAATGGCA CCCCCTGTCC GTCATGCTCC TGTTCCCCTT
    CCTGCCAACC GGAATCACCT TTCCCGCCAG AGCTCTGACC CATCCCCTGA TGGGGTGCCA
    CCCTCTTCCC GTCTAGAGAG GGGACCCTGG GTGAAAAAGG AGGCAGAGGT GCACCCTCCC
    AAGGTTCCCC AGAGAACAAC GTCCATTGCC ACGGCCCTGA ATACGAGTGG TTCTGGGGGC
    AGCAGGCCGG TGCAGGCTGT GAGAGCCAGT AACCCGGACC TGCGACGGGC AGAGAGCGGC
    TGGGAGAGAG GAGACTCTTT GCTGCAGTCC CCCCACCCTA ACCTGCCCCA GGCTGGCTCC
    CTCGAGAGGA ACCGCATAGC CCCCCACAAG CTGGAGGAAT CCTCTATTAG CCCTCCAGGG
    AGCAAAACCC CAGGAGAAGA GCATCGATCC CGCCCTGGGC GCCCAGCAAG CTATAAGAGA
    GCCATAGGAG AGGATCACCT CCTGGTGAAG GACCGGCCCG AAGATGTGCC CAAACCCCCT
    AAGAAAGCGT TGGACTACTC TTCCTCCAGC GATGAACTGG ACAGCAGTGA TGAAGAGGAA
    GAGGAGGGGG ATGCTGAGAG ACAGGAGCCC AACAGGGACA GCTCAGGAGC CAGTCGCAGC
    GGGCGAGACG CCGATACAGA CAGCGTCAGC ACCATGGTGG TTCATGATGT GGAGGAGCTG
    ATGGGGAGCA CGTCTGACAG TTCCTACGGC GACGGCACCA TGGTGGTTCA GAGAACGCCG
    GAGGAGGAGC GCAGTCTCCT TCATGCTGAC AGTAACGGAT ATACCAACCT GCCCGACGTG
    GTGCAACCGA GCCATTCTCC CACCGAGCCC AAGGGACAGA GCACTTCTCC CAGCAAGGAG
    GGCGGCGGCT CTGATTACCA GTCTCGCGGG TTGGTGAAAG CCCCTCCCAA AACCTCATTT
    ACAATGTTTG TGGACCTGGG ACTGTATCAG TCGTCGGGGG GAGGAGGAGA CACCATTCCG
    GTGGCAGCGG CGTACGTGAG CGGAGACCCC GCCCGACTGG AGCAGCTGAA ATACGAAGCC
    CGTAAGGGAT CTGTCGTCAA TGTAAATCCG ACCAATACGA GGCCGCACAG CGACACGCCG
    GAGATCCGCA AGTACAAGAA GAGATTCAAC TCGGAAATCC TTTGCGCCGC TCTCTGGGGT
    GTGAACCTAT TGGTCGGCAC TGAGAATGGA CTGATGCTAC TGGACCGCAG TGGGCAGGGC
    AAAGTATACA CCCTAATTAC CCGCCGGCGC TTCCAGCAGA TGGACGTCCT TGAGGGGCTC
    AACTTGCTCA TTACTATAAG TGGTAAGAGG AACAAGCTGC GGGTGTATTA TCTCTCCTGG
    CTGCGCAACA AGATCCTTCA TAACGACCCC GAGGTGGAGA AGAAGCAGGG CTGGAGCACC
    GTGGGGGACA TGGACGGCTG CGTGCATTAC AAAGTCGTAA AATACGAGCG AATCAAATTC
    CTCGTGATTG CACTGAAAAA CTCCGTCGAG GTTTACGCCT GGGCGCCCAA ACCCTACCAC
    AAGTTTATGG CTTTTAAGTC GTTTACAGAT ATCCCCCACC GGCCCCAGCT GGTCGACCTA
    ACGGTGGAGG AAGGCCAGAG GCTCAAGGTC ATCTACGGTT CCTCGTCGGG ATTCCACGCC
    GTGGATGTCG ACTCCGGGAA CAACTATGAT ATTTACATCC CTGTCCATAT CCAGAGCCAG
    ATTAACCCCC ACGCCATCAT CTTTCTGCCA AACACGGACG GCATGGAGAT GTTACTGTGC
    TACGAGGACG AGGGGGTCTA CGTCAACACC TACGGGCGCA TCATTAAAGA CGTGGTGTTG
    CAGTGGGGAG AGATGCCCAC GTCTGTCGCC TATATCTGCT CCAATCAGAT CATGGGCTGG
    GGGGAGAAGG CCATAGAAAT CCGCTCGGTG GAGACGGGGC ACCTGGACGG GGTCTTCATG
    CACAAGAGAG CTCAGCGGCT GAAGTTCCTG TGTGAGCGCA ACGACAAGGT GTTCTTTGCC
    TCGGTGCGTT CGGGCGGGAG CAGCCAGGTT TACTTCATGA CCCTGAATCG CAGCTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945336.1
    CDS1358..5269
    Translation

    Target ORF information:

    RefSeq Version XM_018089847.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089847.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATATA TCAGGCACAA GTTAGAGGAA GAACAGAGGC AGTTAGAAAT CCTTCAGCAG
    CAGCTTCTGC AGGAGCAGGC ACTCTTACTG GAATACAAGC GGAAGCAGCT GGAGGAGCAG
    AGACAGTCAG AGAGACTGCA GAGACAGCTT CAGCAGGAAC ACGCTTATCT CAAGTCTCTG
    CAGCAGCAGC AACAGCAACA ACAGAAGCAG CAACAGCAGC AGCAGCAGGA TAAGAAGCCG
    CTGTACCATT ACAACCGGGG GGTAATGAAC CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG
    GTGGAGGAGC GTTCGAGGCT CAACAAGCAG AGTTCCCCCC TCTCCATTAC TAAGCTCAGC
    TCGGTGGAGT CTACTGGAAA CCCGTCGCAA ACCCCACCCT CTCAGCGGCC TCCAGAAATA
    CAGGAGCACA AGCCACTACG CATTACTTCT CAGAACCGTG CTGCTCTCAG GCCAGCTCCC
    CGGCCCAACT CCTTGCAGGA CCCAATGGCA CCCCCTGTCC GTCATGCTCC TGTTCCCCTT
    CCTGCCAACC GGAATCACCT TTCCCGCCAG AGCTCTGACC CATCCCCTGA TGGGGTGCCA
    CCCTCTTCCC GTCTAGAGAG GGGACCCTGG GTGAAAAAGG AGGCAGAGGT GCACCCTCCC
    AAGGTTCCCC AGAGAACAAC GTCCATTGCC ACGGCCCTGA ATACGAGTGG TTCTGGGGGC
    AGCAGGCCGG TGCAGGCTGT GAGAGCCAGT AACCCGGACC TGCGACGGGC AGAGAGCGGC
    TGGGAGAGAG GAGACTCTTT GCTGCAGTCC CCCCACCCTA ACCTGCCCCA GGCTGGCTCC
    CTCGAGAGGA ACCGCATAGC CCCCCACAAG CTGGAGGAAT CCTCTATTAG CCCTCCAGGG
    AGCAAAACCC CAGGAGAAGA GCATCGATCC CGCCCTGGGC GCCCAGCAAG CTATAAGAGA
    GCCATAGGAG AGGATCACCT CCTGGTGAAG GACCGGCCCG AAGATGTGCC CAAACCCCCT
    AAGAAAGCGT TGGACTACTC TTCCTCCAGC GATGAACTGG ACAGCAGTGA TGAAGAGGAA
    GAGGAGGGGG ATGCTGAGAG ACAGGAGCCC AACAGGGACA GCTCAGGAGC CAGTCGCAGC
    GGGCGAGACG CCGATACAGA CAGCGTCAGC ACCATGGTGG TTCATGATGT GGAGGAGCTG
    ATGGGGAGCA CGTCTGACAG TTCCTACGGC GACGGCACCA TGGTGGTTCA GAGAACGCCG
    GAGGAGGAGC GCAGTCTCCT TCATGCTGAC AGTAACGGAT ATACCAACCT GCCCGACGTG
    GTGCAACCGA GCCATTCTCC CACCGAGCCC AAGGGACAGA GCACTTCTCC CAGCAAGGAG
    GGCGGCGGCT CTGATTACCA GTCTCGCGGG TTGGTGAAAG CCCCTCCCAA AACCTCATTT
    ACAATGTTTG TGGACCTGGG ACTGTATCAG TCGTCGGGGG GAGGAGGAGA CACCATTCCG
    GTGGCAGCGG CGTACGTGAG CGGAGACCCC GCCCGACTGG AGCAGCTGAA ATACGAAGCC
    CGTAAGGGAT CTGTCGTCAA TGTAAATCCG ACCAATACGA GGCCGCACAG CGACACGCCG
    GAGATCCGCA AGTACAAGAA GAGATTCAAC TCGGAAATCC TTTGCGCCGC TCTCTGGGGT
    GTGAACCTAT TGGTCGGCAC TGAGAATGGA CTGATGCTAC TGGACCGCAG TGGGCAGGGC
    AAAGTATACA CCCTAATTAC CCGCCGGCGC TTCCAGCAGA TGGACGTCCT TGAGGGGCTC
    AACTTGCTCA TTACTATAAG TGGTAAGAGG AACAAGCTGC GGGTGTATTA TCTCTCCTGG
    CTGCGCAACA AGATCCTTCA TAACGACCCC GAGGTGGAGA AGAAGCAGGG CTGGAGCACC
    GTGGGGGACA TGGACGGCTG CGTGCATTAC AAAGTCGTAA AATACGAGCG AATCAAATTC
    CTCGTGATTG CACTGAAAAA CTCCGTCGAG GTTTACGCCT GGGCGCCCAA ACCCTACCAC
    AAGTTTATGG CTTTTAAGTC GTTTACAGAT ATCCCCCACC GGCCCCAGCT GGTCGACCTA
    ACGGTGGAGG AAGGCCAGAG GCTCAAGGTC ATCTACGGTT CCTCGTCGGG ATTCCACGCC
    GTGGATGTCG ACTCCGGGAA CAACTATGAT ATTTACATCC CTGTCCATAT CCAGAGCCAG
    ATTAACCCCC ACGCCATCAT CTTTCTGCCA AACACGGACG GCATGGAGAT GTTACTGTGC
    TACGAGGACG AGGGGGTCTA CGTCAACACC TACGGGCGCA TCATTAAAGA CGTGGTGTTG
    CAGTGGGGAG AGATGCCCAC GTCTGTCGCC TATATCTGCT CCAATCAGAT CATGGGCTGG
    GGGGAGAAGG CCATAGAAAT CCGCTCGGTG GAGACGGGGC ACCTGGACGG GGTCTTCATG
    CACAAGAGAG CTCAGCGGCT GAAGTTCCTG TGTGAGCGCA ACGACAAGGT GTTCTTTGCC
    TCGGTGCGTT CGGGCGGGAG CAGCCAGGTT TACTTCATGA CCCTGAATCG CAGCTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40997
    Clone ID Related Accession (Same CDS sequence) XM_018089850.1
    Accession Version XM_018089850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC CGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGGCCCTTA CTCTGTGTGT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945339.1
    CDS1358..5113
    Translation

    Target ORF information:

    RefSeq Version XM_018089850.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC CGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGGCCCTTA CTCTGTGTGT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40998
    Clone ID Related Accession (Same CDS sequence) XM_018089851.1
    Accession Version XM_018089851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GGTTCCCCAG AGAACAACGT CCATTGCCAC GGCCCTGAAT
    ACGAGTGGTT CTGGGGGCAG CAGGCCGGTG CAGGCTGTGA GAGCCAGTAA CCCGGACCTG
    CGACGGGCAG AGAGCGGCTG GGAGAGAGGA GACTCTTTGC TGCAGTCCCC CCACCCTAAC
    CTGCCCCAGG CTGGCTCCCT CGAGAGGAAC CGCATAGCCC CCCACAAGCT GGAGGAATCC
    TCTATTAGCC CTCCAGGGAG CAAAACCCCA GGAGAAGAGC ATCGATCCCG CCCTGGGCGC
    CCAGCAAGCT ATAAGAGAGC CATAGGAGAG GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945340.1
    CDS1358..5047
    Translation

    Target ORF information:

    RefSeq Version XM_018089851.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC TCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GGTTCCCCAG AGAACAACGT CCATTGCCAC GGCCCTGAAT
    ACGAGTGGTT CTGGGGGCAG CAGGCCGGTG CAGGCTGTGA GAGCCAGTAA CCCGGACCTG
    CGACGGGCAG AGAGCGGCTG GGAGAGAGGA GACTCTTTGC TGCAGTCCCC CCACCCTAAC
    CTGCCCCAGG CTGGCTCCCT CGAGAGGAAC CGCATAGCCC CCCACAAGCT GGAGGAATCC
    TCTATTAGCC CTCCAGGGAG CAAAACCCCA GGAGAAGAGC ATCGATCCCG CCCTGGGCGC
    CCAGCAAGCT ATAAGAGAGC CATAGGAGAG GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53687
    Clone ID Related Accession (Same CDS sequence) XM_031898076.1
    Accession Version XM_031898076.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC AGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGAGAAGGC CATAGAAATC CGCTCGGTGG AGACGGGGCA CCTGGACGGG
    GTCTTCATGC ACAAGAGAGC TCAGCGGCTG AAGTTCCTGT GTGAGCGCAA CGACAAGGTG
    TTCTTTGCCT CGGTGCGTTC GGGCGGGAGC AGCCAGGTTT ACTTCATGAC CCTGAATCGC
    AGCTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753936.1
    CDS1350..5270
    Translation

    Target ORF information:

    RefSeq Version XM_031898076.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC AGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGAGAAGGC CATAGAAATC CGCTCGGTGG AGACGGGGCA CCTGGACGGG
    GTCTTCATGC ACAAGAGAGC TCAGCGGCTG AAGTTCCTGT GTGAGCGCAA CGACAAGGTG
    TTCTTTGCCT CGGTGCGTTC GGGCGGGAGC AGCCAGGTTT ACTTCATGAC CCTGAATCGC
    AGCTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53688
    Clone ID Related Accession (Same CDS sequence) XM_031898077.1
    Accession Version XM_031898077.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGCGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATATA TCAGGCACAA GTTAGAGGAA GAACAGAGGC AGTTAGAAAT CCTTCAGCAG
    CAGCTTCTGC AGGAGCAGGC ACTCTTACTG GAATACAAGC GGAAGCAGCT GGAGGAGCAG
    AGACAGTCAG AGAGACTGCA GAGACAGCTT CAGCAGGAAC ACGCTTATCT CAAGTCTCTG
    CAGCAGCAGC AACAGCAACA ACAGAAGCAG CAACAGCAGC AGCAGCAGGA TAAGAAGCCG
    CTGTACCATT ACAACCGGGG GGTAATGAAC CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG
    GTGGAGGAGC GTTCGAGGCT CAACAAGCAG AGTTCCCCCC TCTCCATTAC TAAGCTCAGC
    TCGGTGGAGT CTACTGGAAA CCCGTCGCAA ACCCCACCCT CCCAGCGGCC TCCAGAAATA
    CAGGAGCACA AGCCACTACG CATTACTTCT CAGAACCGTG CTGCTCTCAG GCCAGCTCCC
    CGGCCCAACT CCTTGCAGGA CCCAATGGCA CCCCCTGTCC GTCATGCTCC TGTTCCCCTT
    CCTGCCAACC GGAATCACCT TTCCCGCCAG AGCTCTGACC CATCCCCTGA TGGGGTGCCA
    CCCTCTTCCC GTCTAGAGAG GGGACCCTGG GTGAAAAAGG AGGCAGAGGT GCACCCTCCC
    AAGGTTCCCC AGAGAACAAC GTCCATTGCC ACGGCCCTGA ATACGAGTGG TTCTGGGGGC
    AGCAGGCCGG TGCAGGCTGT GAGAGCCAGT AACCCGGACC TGCGACGGGC AGAGAGCGGC
    TGGGAGAGAG GAGACTCTTT GCTGCAGTCC CCCCACCCTA ACCTGCCCCA GGCTGGCTCC
    CTCGAGAGGA ACCGCATAGC CCCCCACAAG CTGGAGGAAT CCTCTATTAG CCCTCCAGGG
    AGCAAAACCC CAGGAGAAGA GCATCGATCC CGCCCTGGGC GCCCAGCAAG CTATAAGAGA
    GCCATAGGAG AGGATCACCT CCTGGTGAAG GACCGGCCCG AAGATGTGCC CAAACCCCCT
    AAGAAAGCGT TGGACTACTC TTCCTCCAGC GATGAACTGG ACAGCAGTGA TGAAGAGGAA
    GAGGAGGGGG ATGCTGAGAG ACAGGAGCCC AACAGGGACA GCTCAGGAGC CAGTCGCAGC
    GGGCGAGACG CAGATACAGA CAGCGTCAGC ACCATGGTGG TTCATGATGT GGAGGAGCTG
    ATGGGGAGCA CGTCTGACAG TTCCTACGGC GACGGCACCA TGGTGGTTCA GAGAACGCCG
    GAGGAGGAGC GCAGTCTCCT TCATGCTGAC AGTAACGGAT ATACCAACCT GCCCGACGTG
    GTGCAACCGA GCCATTCTCC CACCGAGCCC AAGGGACAGA GCACTTCTCC CAGCAAGGAG
    GGCGGCGGCT CTGATTACCA GTCTCGCGGG TTGGTGAAAG CCCCTCCCAA AACCTCATTT
    ACAATGTTTG TGGACCTGGG ACTGTATCAG TCGTCGGGGG GAGGAGGAGA CACCATTCCG
    GTGGCAGCGG CGTACGTGAG CGGAGACCCC GCCCGACTGG AGCAGCTGAA ATACGAAGCC
    CGTAAGGGAT CTGTCGTCAA TGTAAATCCG ACCAATACGA GGCCGCACAG CGACACGCCG
    GAGATCCGCA AGTACAAGAA GAGATTCAAC TCGGAAATCC TTTGCGCCGC TCTCTGGGGT
    GTGAACCTAT TGGTCGGCAC TGAGAATGGA CTGATGCTAC TGGACCGCAG TGGGCAGGGC
    AAAGTATACA CCCTAATTAC CCGCCGGCGC TTCCAGCAGA TGGACGTCCT TGAGGGGCTC
    AACTTGCTCA TTACTATAAG TGGTAAGAGG AACAAGCTGC GGGTGTATTA TCTCTCCTGG
    CTGCGCAACA AGATCCTTCA TAACGACCCC GAGGTGGAGA AGAAGCAGGG CTGGAGCACC
    GTGGGGGACA TGGACGGCTG CGTGCATTAC AAAGTCGTAA AATACGAGCG AATCAAATTC
    CTCGTGATTG CACTGAAAAA CTCCGTCGAG GTTTACGCCT GGGCGCCCAA ACCCTACCAC
    AAGTTTATGG CTTTTAAGTC GTTTACAGAT ATCCCCCACC GGCCCCAGCT GGTCGACCTA
    ACGGTGGAGG AAGGCCAGAG GCTCAAGGTC ATCTACGGTT CCTCGTCGGG ATTCCACGCC
    GTGGATGTCG ACTCCGGGAA CAACTATGAT ATTTACATCC CTGTCCATAT CCAGAGCCAG
    ATTAACCCCC ACGCCATCAT CTTTCTGCCA AACACGGACG GCATGGAGAT GTTACTGTGC
    TACGAGGACG AGGGGGTCTA CGTCAACACC TACGGGCGCA TCATTAAAGA CGTGGTGTTG
    CAGTGGGGAG AGATGCCCAC GTCTGTCGCC TATATCTGCT CCAATCAGAT CATGGGCTGG
    GGGGAGAAGG CCATAGAAAT CCGCTCGGTG GAGACGGGGC ACCTGGACGG GGTCTTCATG
    CACAAGAGAG CTCAGCGGCT GAAGTTCCTG TGTGAGCGCA ACGACAAGGT GTTCTTTGCC
    TCGGTGCGTT CGGGCGGGAG CAGCCAGGTT TACTTCATGA CCCTGAATCG CAGCTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753937.1
    CDS1350..5261
    Translation

    Target ORF information:

    RefSeq Version XM_031898077.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898077.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGCGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATATA TCAGGCACAA GTTAGAGGAA GAACAGAGGC AGTTAGAAAT CCTTCAGCAG
    CAGCTTCTGC AGGAGCAGGC ACTCTTACTG GAATACAAGC GGAAGCAGCT GGAGGAGCAG
    AGACAGTCAG AGAGACTGCA GAGACAGCTT CAGCAGGAAC ACGCTTATCT CAAGTCTCTG
    CAGCAGCAGC AACAGCAACA ACAGAAGCAG CAACAGCAGC AGCAGCAGGA TAAGAAGCCG
    CTGTACCATT ACAACCGGGG GGTAATGAAC CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG
    GTGGAGGAGC GTTCGAGGCT CAACAAGCAG AGTTCCCCCC TCTCCATTAC TAAGCTCAGC
    TCGGTGGAGT CTACTGGAAA CCCGTCGCAA ACCCCACCCT CCCAGCGGCC TCCAGAAATA
    CAGGAGCACA AGCCACTACG CATTACTTCT CAGAACCGTG CTGCTCTCAG GCCAGCTCCC
    CGGCCCAACT CCTTGCAGGA CCCAATGGCA CCCCCTGTCC GTCATGCTCC TGTTCCCCTT
    CCTGCCAACC GGAATCACCT TTCCCGCCAG AGCTCTGACC CATCCCCTGA TGGGGTGCCA
    CCCTCTTCCC GTCTAGAGAG GGGACCCTGG GTGAAAAAGG AGGCAGAGGT GCACCCTCCC
    AAGGTTCCCC AGAGAACAAC GTCCATTGCC ACGGCCCTGA ATACGAGTGG TTCTGGGGGC
    AGCAGGCCGG TGCAGGCTGT GAGAGCCAGT AACCCGGACC TGCGACGGGC AGAGAGCGGC
    TGGGAGAGAG GAGACTCTTT GCTGCAGTCC CCCCACCCTA ACCTGCCCCA GGCTGGCTCC
    CTCGAGAGGA ACCGCATAGC CCCCCACAAG CTGGAGGAAT CCTCTATTAG CCCTCCAGGG
    AGCAAAACCC CAGGAGAAGA GCATCGATCC CGCCCTGGGC GCCCAGCAAG CTATAAGAGA
    GCCATAGGAG AGGATCACCT CCTGGTGAAG GACCGGCCCG AAGATGTGCC CAAACCCCCT
    AAGAAAGCGT TGGACTACTC TTCCTCCAGC GATGAACTGG ACAGCAGTGA TGAAGAGGAA
    GAGGAGGGGG ATGCTGAGAG ACAGGAGCCC AACAGGGACA GCTCAGGAGC CAGTCGCAGC
    GGGCGAGACG CAGATACAGA CAGCGTCAGC ACCATGGTGG TTCATGATGT GGAGGAGCTG
    ATGGGGAGCA CGTCTGACAG TTCCTACGGC GACGGCACCA TGGTGGTTCA GAGAACGCCG
    GAGGAGGAGC GCAGTCTCCT TCATGCTGAC AGTAACGGAT ATACCAACCT GCCCGACGTG
    GTGCAACCGA GCCATTCTCC CACCGAGCCC AAGGGACAGA GCACTTCTCC CAGCAAGGAG
    GGCGGCGGCT CTGATTACCA GTCTCGCGGG TTGGTGAAAG CCCCTCCCAA AACCTCATTT
    ACAATGTTTG TGGACCTGGG ACTGTATCAG TCGTCGGGGG GAGGAGGAGA CACCATTCCG
    GTGGCAGCGG CGTACGTGAG CGGAGACCCC GCCCGACTGG AGCAGCTGAA ATACGAAGCC
    CGTAAGGGAT CTGTCGTCAA TGTAAATCCG ACCAATACGA GGCCGCACAG CGACACGCCG
    GAGATCCGCA AGTACAAGAA GAGATTCAAC TCGGAAATCC TTTGCGCCGC TCTCTGGGGT
    GTGAACCTAT TGGTCGGCAC TGAGAATGGA CTGATGCTAC TGGACCGCAG TGGGCAGGGC
    AAAGTATACA CCCTAATTAC CCGCCGGCGC TTCCAGCAGA TGGACGTCCT TGAGGGGCTC
    AACTTGCTCA TTACTATAAG TGGTAAGAGG AACAAGCTGC GGGTGTATTA TCTCTCCTGG
    CTGCGCAACA AGATCCTTCA TAACGACCCC GAGGTGGAGA AGAAGCAGGG CTGGAGCACC
    GTGGGGGACA TGGACGGCTG CGTGCATTAC AAAGTCGTAA AATACGAGCG AATCAAATTC
    CTCGTGATTG CACTGAAAAA CTCCGTCGAG GTTTACGCCT GGGCGCCCAA ACCCTACCAC
    AAGTTTATGG CTTTTAAGTC GTTTACAGAT ATCCCCCACC GGCCCCAGCT GGTCGACCTA
    ACGGTGGAGG AAGGCCAGAG GCTCAAGGTC ATCTACGGTT CCTCGTCGGG ATTCCACGCC
    GTGGATGTCG ACTCCGGGAA CAACTATGAT ATTTACATCC CTGTCCATAT CCAGAGCCAG
    ATTAACCCCC ACGCCATCAT CTTTCTGCCA AACACGGACG GCATGGAGAT GTTACTGTGC
    TACGAGGACG AGGGGGTCTA CGTCAACACC TACGGGCGCA TCATTAAAGA CGTGGTGTTG
    CAGTGGGGAG AGATGCCCAC GTCTGTCGCC TATATCTGCT CCAATCAGAT CATGGGCTGG
    GGGGAGAAGG CCATAGAAAT CCGCTCGGTG GAGACGGGGC ACCTGGACGG GGTCTTCATG
    CACAAGAGAG CTCAGCGGCT GAAGTTCCTG TGTGAGCGCA ACGACAAGGT GTTCTTTGCC
    TCGGTGCGTT CGGGCGGGAG CAGCCAGGTT TACTTCATGA CCCTGAATCG CAGCTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53689
    Clone ID Related Accession (Same CDS sequence) XM_031898078.1
    Accession Version XM_031898078.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAGAT
    CACCTCCTGG TGAAGGACCG GCCCGAAGAT GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC
    TACTCTTCCT CCAGCGATGA ACTGGACAGC AGTGATGAAG AGGAAGAGGA GGGGGATGCT
    GAGAGACAGG AGCCCAACAG GGACAGCTCA GGAGCCAGTC GCAGCGGGCG AGACGCAGAT
    ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTGGAGG AGCTGATGGG GAGCACGTCT
    GACAGTTCCT ACGGCGACGG CACCATGGTG GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT
    CTCCTTCATG CTGACAGTAA CGGATATACC AACCTGCCCG ACGTGGTGCA ACCGAGCCAT
    TCTCCCACCG AGCCCAAGGG ACAGAGCACT TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT
    TACCAGTCTC GCGGGTTGGT GAAAGCCCCT CCCAAAACCT CATTTACAAT GTTTGTGGAC
    CTGGGACTGT ATCAGTCGTC GGGGGGAGGA GGAGACACCA TTCCGGTGGC AGCGGCGTAC
    GTGAGCGGAG ACCCCGCCCG ACTGGAGCAG CTGAAATACG AAGCCCGTAA GGGATCTGTC
    GTCAATGTAA ATCCGACCAA TACGAGGCCG CACAGCGACA CGCCGGAGAT CCGCAAGTAC
    AAGAAGAGAT TCAACTCGGA AATCCTTTGC GCCGCTCTCT GGGGTGTGAA CCTATTGGTC
    GGCACTGAGA ATGGACTGAT GCTACTGGAC CGCAGTGGGC AGGGCAAAGT ATACACCCTA
    ATTACCCGCC GGCGCTTCCA GCAGATGGAC GTCCTTGAGG GGCTCAACTT GCTCATTACT
    ATAAGTGGTA AGAGGAACAA GCTGCGGGTG TATTATCTCT CCTGGCTGCG CAACAAGATC
    CTTCATAACG ACCCCGAGGT GGAGAAGAAG CAGGGCTGGA GCACCGTGGG GGACATGGAC
    GGCTGCGTGC ATTACAAAGT CGTAAAATAC GAGCGAATCA AATTCCTCGT GATTGCACTG
    AAAAACTCCG TCGAGGTTTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT TATGGCTTTT
    AAGTCGTTTA CAGATATCCC CCACCGGCCC CAGCTGGTCG ACCTAACGGT GGAGGAAGGC
    CAGAGGCTCA AGGTCATCTA CGGTTCCTCG TCGGGATTCC ACGCCGTGGA TGTCGACTCC
    GGGAACAACT ATGATATTTA CATCCCTGTC CATATCCAGA GCCAGATTAA CCCCCACGCC
    ATCATCTTTC TGCCAAACAC GGACGGCATG GAGATGTTAC TGTGCTACGA GGACGAGGGG
    GTCTACGTCA ACACCTACGG GCGCATCATT AAAGACGTGG TGTTGCAGTG GGGAGAGATG
    CCCACGTCTG TCGCCTATAT CTGCTCCAAT CAGATCATGG GCTGGGGGGA GAAGGCCATA
    GAAATCCGCT CGGTGGAGAC GGGGCACCTG GACGGGGTCT TCATGCACAA GAGAGCTCAG
    CGGCTGAAGT TCCTGTGTGA GCGCAACGAC AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC
    GGGAGCAGCC AGGTTTACTT CATGACCCTG AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753938.1
    CDS1349..5245
    Translation

    Target ORF information:

    RefSeq Version XM_031898078.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898078.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAGAT
    CACCTCCTGG TGAAGGACCG GCCCGAAGAT GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC
    TACTCTTCCT CCAGCGATGA ACTGGACAGC AGTGATGAAG AGGAAGAGGA GGGGGATGCT
    GAGAGACAGG AGCCCAACAG GGACAGCTCA GGAGCCAGTC GCAGCGGGCG AGACGCAGAT
    ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTGGAGG AGCTGATGGG GAGCACGTCT
    GACAGTTCCT ACGGCGACGG CACCATGGTG GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT
    CTCCTTCATG CTGACAGTAA CGGATATACC AACCTGCCCG ACGTGGTGCA ACCGAGCCAT
    TCTCCCACCG AGCCCAAGGG ACAGAGCACT TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT
    TACCAGTCTC GCGGGTTGGT GAAAGCCCCT CCCAAAACCT CATTTACAAT GTTTGTGGAC
    CTGGGACTGT ATCAGTCGTC GGGGGGAGGA GGAGACACCA TTCCGGTGGC AGCGGCGTAC
    GTGAGCGGAG ACCCCGCCCG ACTGGAGCAG CTGAAATACG AAGCCCGTAA GGGATCTGTC
    GTCAATGTAA ATCCGACCAA TACGAGGCCG CACAGCGACA CGCCGGAGAT CCGCAAGTAC
    AAGAAGAGAT TCAACTCGGA AATCCTTTGC GCCGCTCTCT GGGGTGTGAA CCTATTGGTC
    GGCACTGAGA ATGGACTGAT GCTACTGGAC CGCAGTGGGC AGGGCAAAGT ATACACCCTA
    ATTACCCGCC GGCGCTTCCA GCAGATGGAC GTCCTTGAGG GGCTCAACTT GCTCATTACT
    ATAAGTGGTA AGAGGAACAA GCTGCGGGTG TATTATCTCT CCTGGCTGCG CAACAAGATC
    CTTCATAACG ACCCCGAGGT GGAGAAGAAG CAGGGCTGGA GCACCGTGGG GGACATGGAC
    GGCTGCGTGC ATTACAAAGT CGTAAAATAC GAGCGAATCA AATTCCTCGT GATTGCACTG
    AAAAACTCCG TCGAGGTTTA CGCCTGGGCG CCCAAACCCT ACCACAAGTT TATGGCTTTT
    AAGTCGTTTA CAGATATCCC CCACCGGCCC CAGCTGGTCG ACCTAACGGT GGAGGAAGGC
    CAGAGGCTCA AGGTCATCTA CGGTTCCTCG TCGGGATTCC ACGCCGTGGA TGTCGACTCC
    GGGAACAACT ATGATATTTA CATCCCTGTC CATATCCAGA GCCAGATTAA CCCCCACGCC
    ATCATCTTTC TGCCAAACAC GGACGGCATG GAGATGTTAC TGTGCTACGA GGACGAGGGG
    GTCTACGTCA ACACCTACGG GCGCATCATT AAAGACGTGG TGTTGCAGTG GGGAGAGATG
    CCCACGTCTG TCGCCTATAT CTGCTCCAAT CAGATCATGG GCTGGGGGGA GAAGGCCATA
    GAAATCCGCT CGGTGGAGAC GGGGCACCTG GACGGGGTCT TCATGCACAA GAGAGCTCAG
    CGGCTGAAGT TCCTGTGTGA GCGCAACGAC AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC
    GGGAGCAGCC AGGTTTACTT CATGACCCTG AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53690
    Clone ID Related Accession (Same CDS sequence) XM_031898079.1
    Accession Version XM_031898079.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCCCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA AGCTATAAGA GAGCCATAGG AGAGGATCAC
    CTCCTGGTGA AGGACCGGCC CGAAGATGTG CCCAAACCCC CTAAGAAAGC GTTGGACTAC
    TCTTCCTCCA GCGATGAACT GGACAGCAGT GATGAAGAGG AAGAGGAGGG GGATGCTGAG
    AGACAGGAGC CCAACAGGGA CAGCTCAGGA GCCAGTCGCA GCGGGCGAGA CGCAGATACA
    GACAGCGTCA GCACCATGGT GGTTCATGAT GTGGAGGAGC TGATGGGGAG CACGTCTGAC
    AGTTCCTACG GCGACGGCAC CATGGTGGTT CAGAGAACGC CGGAGGAGGA GCGCAGTCTC
    CTTCATGCTG ACAGTAACGG ATATACCAAC CTGCCCGACG TGGTGCAACC GAGCCATTCT
    CCCACCGAGC CCAAGGGACA GAGCACTTCT CCCAGCAAGG AGGGCGGCGG CTCTGATTAC
    CAGTCTCGCG GGTTGGTGAA AGCCCCTCCC AAAACCTCAT TTACAATGTT TGTGGACCTG
    GGACTGTATC AGTCGTCGGG GGGAGGAGGA GACACCATTC CGGTGGCAGC GGCGTACGTG
    AGCGGAGACC CCGCCCGACT GGAGCAGCTG AAATACGAAG CCCGTAAGGG ATCTGTCGTC
    AATGTAAATC CGACCAATAC GAGGCCGCAC AGCGACACGC CGGAGATCCG CAAGTACAAG
    AAGAGATTCA ACTCGGAAAT CCTTTGCGCC GCTCTCTGGG GTGTGAACCT ATTGGTCGGC
    ACTGAGAATG GACTGATGCT ACTGGACCGC AGTGGGCAGG GCAAAGTATA CACCCTAATT
    ACCCGCCGGC GCTTCCAGCA GATGGACGTC CTTGAGGGGC TCAACTTGCT CATTACTATA
    AGTGGTAAGA GGAACAAGCT GCGGGTGTAT TATCTCTCCT GGCTGCGCAA CAAGATCCTT
    CATAACGACC CCGAGGTGGA GAAGAAGCAG GGCTGGAGCA CCGTGGGGGA CATGGACGGC
    TGCGTGCATT ACAAAGTCGT AAAATACGAG CGAATCAAAT TCCTCGTGAT TGCACTGAAA
    AACTCCGTCG AGGTTTACGC CTGGGCGCCC AAACCCTACC ACAAGTTTAT GGCTTTTAAG
    TCGTTTACAG ATATCCCCCA CCGGCCCCAG CTGGTCGACC TAACGGTGGA GGAAGGCCAG
    AGGCTCAAGG TCATCTACGG TTCCTCGTCG GGATTCCACG CCGTGGATGT CGACTCCGGG
    AACAACTATG ATATTTACAT CCCTGTCCAT ATCCAGAGCC AGATTAACCC CCACGCCATC
    ATCTTTCTGC CAAACACGGA CGGCATGGAG ATGTTACTGT GCTACGAGGA CGAGGGGGTC
    TACGTCAACA CCTACGGGCG CATCATTAAA GACGTGGTGT TGCAGTGGGG AGAGATGCCC
    ACGTCTGTCG CCTATATCTG CTCCAATCAG ATCATGGGCT GGGGGGAGAA GGCCATAGAA
    ATCCGCTCGG TGGAGACGGG GCACCTGGAC GGGGTCTTCA TGCACAAGAG AGCTCAGCGG
    CTGAAGTTCC TGTGTGAGCG CAACGACAAG GTGTTCTTTG CCTCGGTGCG TTCGGGCGGG
    AGCAGCCAGG TTTACTTCAT GACCCTGAAT CGCAGCTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753939.1
    CDS1349..5182
    Translation

    Target ORF information:

    RefSeq Version XM_031898079.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898079.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCCCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA AGCTATAAGA GAGCCATAGG AGAGGATCAC
    CTCCTGGTGA AGGACCGGCC CGAAGATGTG CCCAAACCCC CTAAGAAAGC GTTGGACTAC
    TCTTCCTCCA GCGATGAACT GGACAGCAGT GATGAAGAGG AAGAGGAGGG GGATGCTGAG
    AGACAGGAGC CCAACAGGGA CAGCTCAGGA GCCAGTCGCA GCGGGCGAGA CGCAGATACA
    GACAGCGTCA GCACCATGGT GGTTCATGAT GTGGAGGAGC TGATGGGGAG CACGTCTGAC
    AGTTCCTACG GCGACGGCAC CATGGTGGTT CAGAGAACGC CGGAGGAGGA GCGCAGTCTC
    CTTCATGCTG ACAGTAACGG ATATACCAAC CTGCCCGACG TGGTGCAACC GAGCCATTCT
    CCCACCGAGC CCAAGGGACA GAGCACTTCT CCCAGCAAGG AGGGCGGCGG CTCTGATTAC
    CAGTCTCGCG GGTTGGTGAA AGCCCCTCCC AAAACCTCAT TTACAATGTT TGTGGACCTG
    GGACTGTATC AGTCGTCGGG GGGAGGAGGA GACACCATTC CGGTGGCAGC GGCGTACGTG
    AGCGGAGACC CCGCCCGACT GGAGCAGCTG AAATACGAAG CCCGTAAGGG ATCTGTCGTC
    AATGTAAATC CGACCAATAC GAGGCCGCAC AGCGACACGC CGGAGATCCG CAAGTACAAG
    AAGAGATTCA ACTCGGAAAT CCTTTGCGCC GCTCTCTGGG GTGTGAACCT ATTGGTCGGC
    ACTGAGAATG GACTGATGCT ACTGGACCGC AGTGGGCAGG GCAAAGTATA CACCCTAATT
    ACCCGCCGGC GCTTCCAGCA GATGGACGTC CTTGAGGGGC TCAACTTGCT CATTACTATA
    AGTGGTAAGA GGAACAAGCT GCGGGTGTAT TATCTCTCCT GGCTGCGCAA CAAGATCCTT
    CATAACGACC CCGAGGTGGA GAAGAAGCAG GGCTGGAGCA CCGTGGGGGA CATGGACGGC
    TGCGTGCATT ACAAAGTCGT AAAATACGAG CGAATCAAAT TCCTCGTGAT TGCACTGAAA
    AACTCCGTCG AGGTTTACGC CTGGGCGCCC AAACCCTACC ACAAGTTTAT GGCTTTTAAG
    TCGTTTACAG ATATCCCCCA CCGGCCCCAG CTGGTCGACC TAACGGTGGA GGAAGGCCAG
    AGGCTCAAGG TCATCTACGG TTCCTCGTCG GGATTCCACG CCGTGGATGT CGACTCCGGG
    AACAACTATG ATATTTACAT CCCTGTCCAT ATCCAGAGCC AGATTAACCC CCACGCCATC
    ATCTTTCTGC CAAACACGGA CGGCATGGAG ATGTTACTGT GCTACGAGGA CGAGGGGGTC
    TACGTCAACA CCTACGGGCG CATCATTAAA GACGTGGTGT TGCAGTGGGG AGAGATGCCC
    ACGTCTGTCG CCTATATCTG CTCCAATCAG ATCATGGGCT GGGGGGAGAA GGCCATAGAA
    ATCCGCTCGG TGGAGACGGG GCACCTGGAC GGGGTCTTCA TGCACAAGAG AGCTCAGCGG
    CTGAAGTTCC TGTGTGAGCG CAACGACAAG GTGTTCTTTG CCTCGGTGCG TTCGGGCGGG
    AGCAGCCAGG TTTACTTCAT GACCCTGAAT CGCAGCTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53691
    Clone ID Related Accession (Same CDS sequence) XM_031898080.1
    Accession Version XM_031898080.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCCCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753940.1
    CDS1349..5158
    Translation

    Target ORF information:

    RefSeq Version XM_031898080.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898080.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCCCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53692
    Clone ID Related Accession (Same CDS sequence) XM_018089850.2
    Accession Version XM_018089850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC AGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGGCCCTTA CTCTGTGTGT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945339.1
    CDS1350..5105
    Translation

    Target ORF information:

    RefSeq Version XM_018089850.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GCCACTACGC ATTACTTCTC AGAACCGTGC TGCTCTCAGG
    CCAGCTCCCC GGCCCAACTC CTTGCAGGAC CCAATGGCAC CCCCTGTCCG TCATGCTCCT
    GTTCCCCTTC CTGCCAACCG GAATCACCTT TCCCGCCAGA GCTCTGACCC ATCCCCTGAT
    GGGGTGCCAC CCTCTTCCCG TCTAGAGAGG GGACCCTGGG TGAAAAAGGA GGCAGAGGTG
    CACCCTCCCA AGGTTCCCCA GAGAACAACG TCCATTGCCA CGGCCCTGAA TACGAGTGGT
    TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG AGAGCCAGTA ACCCGGACCT GCGACGGGCA
    GAGAGCGGCT GGGAGAGAGG AGACTCTTTG CTGCAGTCCC CCCACCCTAA CCTGCCCCAG
    GCTGGCTCCC TCGAGAGGAA CCGCATAGCC CCCCACAAGC TGGAGGAATC CTCTATTAGC
    CCTCCAGGGA GCAAAACCCC AGGAGAAGAG CATCGATCCC GCCCTGGGCG CCCAGCAAGC
    TATAAGAGAG CCATAGGAGA GGATCACCTC CTGGTGAAGG ACCGGCCCGA AGATGTGCCC
    AAACCCCCTA AGAAAGCGTT GGACTACTCT TCCTCCAGCG ATGAACTGGA CAGCAGTGAT
    GAAGAGGAAG AGGAGGGGGA TGCTGAGAGA CAGGAGCCCA ACAGGGACAG CTCAGGAGCC
    AGTCGCAGCG GGCGAGACGC AGATACAGAC AGCGTCAGCA CCATGGTGGT TCATGATGTG
    GAGGAGCTGA TGGGGAGCAC GTCTGACAGT TCCTACGGCG ACGGCACCAT GGTGGTTCAG
    AGAACGCCGG AGGAGGAGCG CAGTCTCCTT CATGCTGACA GTAACGGATA TACCAACCTG
    CCCGACGTGG TGCAACCGAG CCATTCTCCC ACCGAGCCCA AGGGACAGAG CACTTCTCCC
    AGCAAGGAGG GCGGCGGCTC TGATTACCAG TCTCGCGGGT TGGTGAAAGC CCCTCCCAAA
    ACCTCATTTA CAATGTTTGT GGACCTGGGA CTGTATCAGT CGTCGGGGGG AGGAGGAGAC
    ACCATTCCGG TGGCAGCGGC GTACGTGAGC GGAGACCCCG CCCGACTGGA GCAGCTGAAA
    TACGAAGCCC GTAAGGGATC TGTCGTCAAT GTAAATCCGA CCAATACGAG GCCGCACAGC
    GACACGCCGG AGATCCGCAA GTACAAGAAG AGATTCAACT CGGAAATCCT TTGCGCCGCT
    CTCTGGGGTG TGAACCTATT GGTCGGCACT GAGAATGGAC TGATGCTACT GGACCGCAGT
    GGGCAGGGCA AAGTATACAC CCTAATTACC CGCCGGCGCT TCCAGCAGAT GGACGTCCTT
    GAGGGGCTCA ACTTGCTCAT TACTATAAGT GGTAAGAGGA ACAAGCTGCG GGTGTATTAT
    CTCTCCTGGC TGCGCAACAA GATCCTTCAT AACGACCCCG AGGTGGAGAA GAAGCAGGGC
    TGGAGCACCG TGGGGGACAT GGACGGCTGC GTGCATTACA AAGTCGTAAA ATACGAGCGA
    ATCAAATTCC TCGTGATTGC ACTGAAAAAC TCCGTCGAGG TTTACGCCTG GGCGCCCAAA
    CCCTACCACA AGTTTATGGC TTTTAAGTCG TTTACAGATA TCCCCCACCG GCCCCAGCTG
    GTCGACCTAA CGGTGGAGGA AGGCCAGAGG CTCAAGGTCA TCTACGGTTC CTCGTCGGGA
    TTCCACGCCG TGGATGTCGA CTCCGGGAAC AACTATGATA TTTACATCCC TGTCCATATC
    CAGAGCCAGA TTAACCCCCA CGCCATCATC TTTCTGCCAA ACACGGACGG CATGGAGATG
    TTACTGTGCT ACGAGGACGA GGGGGTCTAC GTCAACACCT ACGGGCGCAT CATTAAAGAC
    GTGGTGTTGC AGTGGGGAGA GATGCCCACG TCTGTCGCCT ATATCTGCTC CAATCAGATC
    ATGGGCTGGG GGGGCCCTTA CTCTGTGTGT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53693
    Clone ID Related Accession (Same CDS sequence) XM_031898081.1
    Accession Version XM_031898081.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GGTTCCCCAG AGAACAACGT CCATTGCCAC GGCCCTGAAT
    ACGAGTGGTT CTGGGGGCAG CAGGCCGGTG CAGGCTGTGA GAGCCAGTAA CCCGGACCTG
    CGACGGGCAG AGAGCGGCTG GGAGAGAGGA GACTCTTTGC TGCAGTCCCC CCACCCTAAC
    CTGCCCCAGG CTGGCTCCCT CGAGAGGAAC CGCATAGCCC CCCACAAGCT GGAGGAATCC
    TCTATTAGCC CTCCAGGGAG CAAAACCCCA GGAGAAGAGC ATCGATCCCG CCCTGGGCGC
    CCAGCAAGCT ATAAGAGAGC CATAGGAGAG GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753941.1
    CDS1349..5038
    Translation

    Target ORF information:

    RefSeq Version XM_031898081.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898081.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGTGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGCGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATATAT CAGGCACAAG TTAGAGGAAG AACAGAGGCA GTTAGAAATC
    CTTCAGCAGC AGCTTCTGCA GGAGCAGGCA CTCTTACTGG AATACAAGCG GAAGCAGCTG
    GAGGAGCAGA GACAGTCAGA GAGACTGCAG AGACAGCTTC AGCAGGAACA CGCTTATCTC
    AAGTCTCTGC AGCAGCAGCA ACAGCAACAA CAGAAGCAGC AACAGCAGCA GCAGCAGGAT
    AAGAAGCCGC TGTACCATTA CAACCGGGGG GTAATGAACC CCAGTGAGAA GCCGGCCTGG
    GCTAGAGAGG TGGAGGAGCG TTCGAGGCTC AACAAGCAGA GTTCCCCCCT CTCCATTACT
    AAGCTCAGCT CGGTGGAGTC TACTGGAAAC CCGTCGCAAA CCCCACCCTC CCAGCGGCCT
    CCAGAAATAC AGGAGCACAA GGTTCCCCAG AGAACAACGT CCATTGCCAC GGCCCTGAAT
    ACGAGTGGTT CTGGGGGCAG CAGGCCGGTG CAGGCTGTGA GAGCCAGTAA CCCGGACCTG
    CGACGGGCAG AGAGCGGCTG GGAGAGAGGA GACTCTTTGC TGCAGTCCCC CCACCCTAAC
    CTGCCCCAGG CTGGCTCCCT CGAGAGGAAC CGCATAGCCC CCCACAAGCT GGAGGAATCC
    TCTATTAGCC CTCCAGGGAG CAAAACCCCA GGAGAAGAGC ATCGATCCCG CCCTGGGCGC
    CCAGCAAGCT ATAAGAGAGC CATAGGAGAG GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa53694
    Clone ID Related Accession (Same CDS sequence) XM_031898082.1
    Accession Version XM_031898082.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGCGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCCCAGCG GCCTCCAGAA ATACAGGAGC ACAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031753942.1
    CDS1349..4918
    Translation

    Target ORF information:

    RefSeq Version XM_031898082.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898082.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGA
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGCGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCCCAGCG GCCTCCAGAA ATACAGGAGC ACAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCA GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa22550
    Clone ID Related Accession (Same CDS sequence) NM_001079271.1
    Accession Version NM_001079271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125722.1. ##Evidence-Data-START## Transcript exon combination :: BC125722.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGGA TAAGAAGCCG CTGTACCATT ACAACCGGGG GGTAATGAAC
    CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG GTGGAGGAGC GTTCGAGGCT CAACAAGCAG
    AGTTCCCCCC TCTCCATTAC TAAGCTCAGC TCGGTGGAGT CTACTGGAAA CCCGTCGCAA
    ACCCCACCCT CACAGCGGCC TCCAGAAATA CAGGAGCACA AGGTTCCCCA GAGAACAACG
    TCCATTGCCA CGGCCCTGAA TACGAGTGGT TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG
    AGAGCCAGTA ACCCGGACCT GCGACGGGCA GAGAGCGGCT GGGAGAGAGG AGACTCTTTG
    CTGCAGTCCC CCCACCCTAA CCTGCCCCAG GCTGGCTCCC TCGAGAGGAA CCGCATAGCC
    CCCCACAAGC TGGAGGAATC CTCTATTAGC CCTCCAGGGA GCAAAACCCC AGGAGAAGAG
    CATCGATCCC GCCCTGGGCG CCCAGCAGAT CACCTCCTGG TGAAGGACCG GCCCGAAGAT
    GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC TACTCTTCCT CCAGCGATGA ACTGGACAGC
    AGTGATGAAG AGGAAGAGGA GGGCGATGCT GAGAGACAGG AGCCCAACAG GGACAGCTCA
    GGAGCCAGTC GCAGCGGGCG AGATGCCGAT ACAGACAGCG TCAGCACCAT GGTGGTTCAT
    GATGTGGAGG AGCTGATGGG GAGCACGTCT GACAGTTCCT ATGGCGATGG CACCATGGTG
    GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT CTCCTTCATG CTGACAGTAA CGGATATACC
    AACCTGCCCG ACGTGGTGCA ACCGAGCCAT TCTCCCACCG AGCCCAAGGG ACAGAGCACT
    TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT TACCAGTCTC GCGGGTTGGT GAAAGCCCCT
    CCCAAAACCT CATTTACAAT GTTTGTGGAC CTGGGACTGT ATCAGTCGTC GGGGGGAGGA
    GGAGACACCA TTCCGGTGGC AGCGGCGTAC GTGAGTGGAG ACCCCGCCCG ACTGGAGCAG
    CTGAAATATG AAGCCCGTAA GGGATCCGTC GTCAATGTAA ATCCGACCAA TACGAGGCCG
    CACAGCGACA CGCCGGAGAT CCGCAAGTAC AAGAAGAGAT TCAACTCGGA AATCCTTTGC
    GCTGCTCTCT GGGGTGTGAA CCTATTGGTC GGCACTGAGA ACGGACTGAT GCTACTGGAC
    CGCAGTGGGC AGGGCAAAGT ATACACCCTA ATTACCCGCC GGCGCTTCCA GCAGATGGAC
    GTCCTCGAGG GGCTCAACTT GCTCATTACT ATAAGTGGTA AGAGGAACAA GCTGCGGGTG
    TATTATCTCT CCTGGCTGCG CAACAAGATC CTTCATAACG ACCCCGAGGT GGAGAAGAAG
    CAGGGCTGGA GCACCGTGGG GGACATGGAC GGCTGCGTGC ATTACAAAGT CGTAAAATAC
    GAGCGAATCA AATTCCTCGT GATTGCACTG AAAAACTCCG TCGAGGTTTA CGCCTGGGCG
    CCCAAACCCT ACCACAAGTT TATGGCTTTT AAGTCGTTTA CAGATATCCC CCACCGGCCC
    CAGCTGGTCG ACCTAACGGT GGAGGAAGGC CAGAGGCTCA AGGTCATCTA CGGTTCCTCG
    TCGGGATTCC ACGCCGTGGA TGTCGACTCC GGGAACAACT ATGATATTTA CATCCCTGTC
    CATATCCAGA GCCAGATTAA CCCCCACGCC ATCATCTTTC TGCCAAACAC GGACGGCATG
    GAGATGTTAC TGTGCTACGA GGACGAGGGG GTCTACGTCA ACACCTACGG GCGCATCATT
    AAAGACGTGG TGTTGCAGTG GGGAGAGATG CCCACGTCTG TCGCCTATAT CTGCTCCAAT
    CAGATCATGG GCTGGGGGGA GAAGGCCATA GAAATCCGCT CGGTGGAGAC GGGGCACCTG
    GACGGGGTCT TCATGCACAA GAGAGCTCAG CGGCTGAAGT TCCTGTGTGA GCGCAACGAC
    AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC GGGAGCAGCC AGGTTTACTT CATGACCCTG
    AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072739.1
    CDS151..3717
    Translation

    Target ORF information:

    RefSeq Version NM_001079271.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGTGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGGA TAAGAAGCCG CTGTACCATT ACAACCGGGG GGTAATGAAC
    CCCAGTGAGA AGCCGGCCTG GGCTAGAGAG GTGGAGGAGC GTTCGAGGCT CAACAAGCAG
    AGTTCCCCCC TCTCCATTAC TAAGCTCAGC TCGGTGGAGT CTACTGGAAA CCCGTCGCAA
    ACCCCACCCT CACAGCGGCC TCCAGAAATA CAGGAGCACA AGGTTCCCCA GAGAACAACG
    TCCATTGCCA CGGCCCTGAA TACGAGTGGT TCTGGGGGCA GCAGGCCGGT GCAGGCTGTG
    AGAGCCAGTA ACCCGGACCT GCGACGGGCA GAGAGCGGCT GGGAGAGAGG AGACTCTTTG
    CTGCAGTCCC CCCACCCTAA CCTGCCCCAG GCTGGCTCCC TCGAGAGGAA CCGCATAGCC
    CCCCACAAGC TGGAGGAATC CTCTATTAGC CCTCCAGGGA GCAAAACCCC AGGAGAAGAG
    CATCGATCCC GCCCTGGGCG CCCAGCAGAT CACCTCCTGG TGAAGGACCG GCCCGAAGAT
    GTGCCCAAAC CCCCTAAGAA AGCGTTGGAC TACTCTTCCT CCAGCGATGA ACTGGACAGC
    AGTGATGAAG AGGAAGAGGA GGGCGATGCT GAGAGACAGG AGCCCAACAG GGACAGCTCA
    GGAGCCAGTC GCAGCGGGCG AGATGCCGAT ACAGACAGCG TCAGCACCAT GGTGGTTCAT
    GATGTGGAGG AGCTGATGGG GAGCACGTCT GACAGTTCCT ATGGCGATGG CACCATGGTG
    GTTCAGAGAA CGCCGGAGGA GGAGCGCAGT CTCCTTCATG CTGACAGTAA CGGATATACC
    AACCTGCCCG ACGTGGTGCA ACCGAGCCAT TCTCCCACCG AGCCCAAGGG ACAGAGCACT
    TCTCCCAGCA AGGAGGGCGG CGGCTCTGAT TACCAGTCTC GCGGGTTGGT GAAAGCCCCT
    CCCAAAACCT CATTTACAAT GTTTGTGGAC CTGGGACTGT ATCAGTCGTC GGGGGGAGGA
    GGAGACACCA TTCCGGTGGC AGCGGCGTAC GTGAGTGGAG ACCCCGCCCG ACTGGAGCAG
    CTGAAATATG AAGCCCGTAA GGGATCCGTC GTCAATGTAA ATCCGACCAA TACGAGGCCG
    CACAGCGACA CGCCGGAGAT CCGCAAGTAC AAGAAGAGAT TCAACTCGGA AATCCTTTGC
    GCTGCTCTCT GGGGTGTGAA CCTATTGGTC GGCACTGAGA ACGGACTGAT GCTACTGGAC
    CGCAGTGGGC AGGGCAAAGT ATACACCCTA ATTACCCGCC GGCGCTTCCA GCAGATGGAC
    GTCCTCGAGG GGCTCAACTT GCTCATTACT ATAAGTGGTA AGAGGAACAA GCTGCGGGTG
    TATTATCTCT CCTGGCTGCG CAACAAGATC CTTCATAACG ACCCCGAGGT GGAGAAGAAG
    CAGGGCTGGA GCACCGTGGG GGACATGGAC GGCTGCGTGC ATTACAAAGT CGTAAAATAC
    GAGCGAATCA AATTCCTCGT GATTGCACTG AAAAACTCCG TCGAGGTTTA CGCCTGGGCG
    CCCAAACCCT ACCACAAGTT TATGGCTTTT AAGTCGTTTA CAGATATCCC CCACCGGCCC
    CAGCTGGTCG ACCTAACGGT GGAGGAAGGC CAGAGGCTCA AGGTCATCTA CGGTTCCTCG
    TCGGGATTCC ACGCCGTGGA TGTCGACTCC GGGAACAACT ATGATATTTA CATCCCTGTC
    CATATCCAGA GCCAGATTAA CCCCCACGCC ATCATCTTTC TGCCAAACAC GGACGGCATG
    GAGATGTTAC TGTGCTACGA GGACGAGGGG GTCTACGTCA ACACCTACGG GCGCATCATT
    AAAGACGTGG TGTTGCAGTG GGGAGAGATG CCCACGTCTG TCGCCTATAT CTGCTCCAAT
    CAGATCATGG GCTGGGGGGA GAAGGCCATA GAAATCCGCT CGGTGGAGAC GGGGCACCTG
    GACGGGGTCT TCATGCACAA GAGAGCTCAG CGGCTGAAGT TCCTGTGTGA GCGCAACGAC
    AAGGTGTTCT TTGCCTCGGT GCGTTCGGGC GGGAGCAGCC AGGTTTACTT CATGACCCTG
    AATCGCAGCT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29571
    Clone ID Related Accession (Same CDS sequence) XM_012953860.2
    Accession Version XM_012953860.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCTCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809314.1
    CDS1357..5166
    Translation

    Target ORF information:

    RefSeq Version XM_012953860.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCTCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29574
    Clone ID Related Accession (Same CDS sequence) XM_012953863.2
    Accession Version XM_012953863.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCTCAGCG GCCTCCAGAA ATACAGGAGC ACAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809317.1
    CDS1357..4926
    Translation

    Target ORF information:

    RefSeq Version XM_012953863.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953863.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCTCAGCG GCCTCCAGAA ATACAGGAGC ACAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA GATCACCTCC TGGTGAAGGA CCGGCCCGAA
    GATGTGCCCA AACCCCCTAA GAAAGCGTTG GACTACTCTT CCTCCAGCGA TGAACTGGAC
    AGCAGTGATG AAGAGGAAGA GGAGGGGGAT GCTGAGAGAC AGGAGCCCAA CAGGGACAGC
    TCAGGAGCCA GTCGCAGCGG GCGAGACGCC GATACAGACA GCGTCAGCAC CATGGTGGTT
    CATGATGTGG AGGAGCTGAT GGGGAGCACG TCTGACAGTT CCTACGGCGA CGGCACCATG
    GTGGTTCAGA GAACGCCGGA GGAGGAGCGC AGTCTCCTTC ATGCTGACAG TAACGGATAT
    ACCAACCTGC CCGACGTGGT GCAACCGAGC CATTCTCCCA CCGAGCCCAA GGGACAGAGC
    ACTTCTCCCA GCAAGGAGGG CGGCGGCTCT GATTACCAGT CTCGCGGGTT GGTGAAAGCC
    CCTCCCAAAA CCTCATTTAC AATGTTTGTG GACCTGGGAC TGTATCAGTC GTCGGGGGGA
    GGAGGAGACA CCATTCCGGT GGCAGCGGCG TACGTGAGCG GAGACCCCGC CCGACTGGAG
    CAGCTGAAAT ACGAAGCCCG TAAGGGATCT GTCGTCAATG TAAATCCGAC CAATACGAGG
    CCGCACAGCG ACACGCCGGA GATCCGCAAG TACAAGAAGA GATTCAACTC GGAAATCCTT
    TGCGCCGCTC TCTGGGGTGT GAACCTATTG GTCGGCACTG AGAATGGACT GATGCTACTG
    GACCGCAGTG GGCAGGGCAA AGTATACACC CTAATTACCC GCCGGCGCTT CCAGCAGATG
    GACGTCCTTG AGGGGCTCAA CTTGCTCATT ACTATAAGTG GTAAGAGGAA CAAGCTGCGG
    GTGTATTATC TCTCCTGGCT GCGCAACAAG ATCCTTCATA ACGACCCCGA GGTGGAGAAG
    AAGCAGGGCT GGAGCACCGT GGGGGACATG GACGGCTGCG TGCATTACAA AGTCGTAAAA
    TACGAGCGAA TCAAATTCCT CGTGATTGCA CTGAAAAACT CCGTCGAGGT TTACGCCTGG
    GCGCCCAAAC CCTACCACAA GTTTATGGCT TTTAAGTCGT TTACAGATAT CCCCCACCGG
    CCCCAGCTGG TCGACCTAAC GGTGGAGGAA GGCCAGAGGC TCAAGGTCAT CTACGGTTCC
    TCGTCGGGAT TCCACGCCGT GGATGTCGAC TCCGGGAACA ACTATGATAT TTACATCCCT
    GTCCATATCC AGAGCCAGAT TAACCCCCAC GCCATCATCT TTCTGCCAAA CACGGACGGC
    ATGGAGATGT TACTGTGCTA CGAGGACGAG GGGGTCTACG TCAACACCTA CGGGCGCATC
    ATTAAAGACG TGGTGTTGCA GTGGGGAGAG ATGCCCACGT CTGTCGCCTA TATCTGCTCC
    AATCAGATCA TGGGCTGGGG GGAGAAGGCC ATAGAAATCC GCTCGGTGGA GACGGGGCAC
    CTGGACGGGG TCTTCATGCA CAAGAGAGCT CAGCGGCTGA AGTTCCTGTG TGAGCGCAAC
    GACAAGGTGT TCTTTGCCTC GGTGCGTTCG GGCGGGAGCA GCCAGGTTTA CTTCATGACC
    CTGAATCGCA GCTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40995
    Clone ID Related Accession (Same CDS sequence) XM_018089848.1
    Accession Version XM_018089848.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCTCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA AGCTATAAGA GAGCCATAGG AGAGGATCAC
    CTCCTGGTGA AGGACCGGCC CGAAGATGTG CCCAAACCCC CTAAGAAAGC GTTGGACTAC
    TCTTCCTCCA GCGATGAACT GGACAGCAGT GATGAAGAGG AAGAGGAGGG GGATGCTGAG
    AGACAGGAGC CCAACAGGGA CAGCTCAGGA GCCAGTCGCA GCGGGCGAGA CGCCGATACA
    GACAGCGTCA GCACCATGGT GGTTCATGAT GTGGAGGAGC TGATGGGGAG CACGTCTGAC
    AGTTCCTACG GCGACGGCAC CATGGTGGTT CAGAGAACGC CGGAGGAGGA GCGCAGTCTC
    CTTCATGCTG ACAGTAACGG ATATACCAAC CTGCCCGACG TGGTGCAACC GAGCCATTCT
    CCCACCGAGC CCAAGGGACA GAGCACTTCT CCCAGCAAGG AGGGCGGCGG CTCTGATTAC
    CAGTCTCGCG GGTTGGTGAA AGCCCCTCCC AAAACCTCAT TTACAATGTT TGTGGACCTG
    GGACTGTATC AGTCGTCGGG GGGAGGAGGA GACACCATTC CGGTGGCAGC GGCGTACGTG
    AGCGGAGACC CCGCCCGACT GGAGCAGCTG AAATACGAAG CCCGTAAGGG ATCTGTCGTC
    AATGTAAATC CGACCAATAC GAGGCCGCAC AGCGACACGC CGGAGATCCG CAAGTACAAG
    AAGAGATTCA ACTCGGAAAT CCTTTGCGCC GCTCTCTGGG GTGTGAACCT ATTGGTCGGC
    ACTGAGAATG GACTGATGCT ACTGGACCGC AGTGGGCAGG GCAAAGTATA CACCCTAATT
    ACCCGCCGGC GCTTCCAGCA GATGGACGTC CTTGAGGGGC TCAACTTGCT CATTACTATA
    AGTGGTAAGA GGAACAAGCT GCGGGTGTAT TATCTCTCCT GGCTGCGCAA CAAGATCCTT
    CATAACGACC CCGAGGTGGA GAAGAAGCAG GGCTGGAGCA CCGTGGGGGA CATGGACGGC
    TGCGTGCATT ACAAAGTCGT AAAATACGAG CGAATCAAAT TCCTCGTGAT TGCACTGAAA
    AACTCCGTCG AGGTTTACGC CTGGGCGCCC AAACCCTACC ACAAGTTTAT GGCTTTTAAG
    TCGTTTACAG ATATCCCCCA CCGGCCCCAG CTGGTCGACC TAACGGTGGA GGAAGGCCAG
    AGGCTCAAGG TCATCTACGG TTCCTCGTCG GGATTCCACG CCGTGGATGT CGACTCCGGG
    AACAACTATG ATATTTACAT CCCTGTCCAT ATCCAGAGCC AGATTAACCC CCACGCCATC
    ATCTTTCTGC CAAACACGGA CGGCATGGAG ATGTTACTGT GCTACGAGGA CGAGGGGGTC
    TACGTCAACA CCTACGGGCG CATCATTAAA GACGTGGTGT TGCAGTGGGG AGAGATGCCC
    ACGTCTGTCG CCTATATCTG CTCCAATCAG ATCATGGGCT GGGGGGAGAA GGCCATAGAA
    ATCCGCTCGG TGGAGACGGG GCACCTGGAC GGGGTCTTCA TGCACAAGAG AGCTCAGCGG
    CTGAAGTTCC TGTGTGAGCG CAACGACAAG GTGTTCTTTG CCTCGGTGCG TTCGGGCGGG
    AGCAGCCAGG TTTACTTCAT GACCCTGAAT CGCAGCTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945337.1
    CDS1358..5191
    Translation

    Target ORF information:

    RefSeq Version XM_018089848.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG GTGAGAAAGA TGAGACAGAG
    TATGAATACA GTGGCAGTGA GGATGAGGAG GAGAACCACG GAGAGGAAGG AGAACCAAGT
    TCCATAATGA ACGTGCCGGG GGAATCCACT CTGAGACGCG AGTTCCTGAG GCTGCAGCAG
    GAGAACAAAA GTAACTCAGA AGCCCTAAAG CAGCAGCAGA TAGGCGCCCA GCACCGCGAC
    TCCGAGGCAC ATATCAAGCA GCTGCTCCAT GAGAGGCAGA GGCGCATAGA GGAGCAAAAG
    GAAGAGCGCC GGCGAGTGGA AGAGCAACAG CGGCGGGAGA GAGAGCAGAG GAAGCAGCAG
    GAGAAGGAGC AGCAAAGGCG CCGTGATGAC TTACGGCGGG AGGAAGAGAG GAGGATGGCA
    GAGAGAGAGC AGGAATACAA GCGGAAGCAG CTGGAGGAGC AGAGACAGTC AGAGAGACTG
    CAGAGACAGC TTCAGCAGGA ACACGCTTAT CTCAAGTCTC TGCAGCAGCA GCAACAGCAA
    CAACAGAAGC AGCAACAGCA GCAGCAGCAG GATAAGAAGC CGCTGTACCA TTACAACCGG
    GGGGTAATGA ACCCCAGTGA GAAGCCGGCC TGGGCTAGAG AGGTGGAGGA GCGTTCGAGG
    CTCAACAAGC AGAGTTCCCC CCTCTCCATT ACTAAGCTCA GCTCGGTGGA GTCTACTGGA
    AACCCGTCGC AAACCCCACC CTCTCAGCGG CCTCCAGAAA TACAGGAGCA CAAGCCACTA
    CGCATTACTT CTCAGAACCG TGCTGCTCTC AGGCCAGCTC CCCGGCCCAA CTCCTTGCAG
    GACCCAATGG CACCCCCTGT CCGTCATGCT CCTGTTCCCC TTCCTGCCAA CCGGAATCAC
    CTTTCCCGCC AGAGCTCTGA CCCATCCCCT GATGGGGTGC CACCCTCTTC CCGTCTAGAG
    AGGGGACCCT GGGTGAAAAA GGAGGCAGAG GTGCACCCTC CCAAGGTTCC CCAGAGAACA
    ACGTCCATTG CCACGGCCCT GAATACGAGT GGTTCTGGGG GCAGCAGGCC GGTGCAGGCT
    GTGAGAGCCA GTAACCCGGA CCTGCGACGG GCAGAGAGCG GCTGGGAGAG AGGAGACTCT
    TTGCTGCAGT CCCCCCACCC TAACCTGCCC CAGGCTGGCT CCCTCGAGAG GAACCGCATA
    GCCCCCCACA AGCTGGAGGA ATCCTCTATT AGCCCTCCAG GGAGCAAAAC CCCAGGAGAA
    GAGCATCGAT CCCGCCCTGG GCGCCCAGCA AGCTATAAGA GAGCCATAGG AGAGGATCAC
    CTCCTGGTGA AGGACCGGCC CGAAGATGTG CCCAAACCCC CTAAGAAAGC GTTGGACTAC
    TCTTCCTCCA GCGATGAACT GGACAGCAGT GATGAAGAGG AAGAGGAGGG GGATGCTGAG
    AGACAGGAGC CCAACAGGGA CAGCTCAGGA GCCAGTCGCA GCGGGCGAGA CGCCGATACA
    GACAGCGTCA GCACCATGGT GGTTCATGAT GTGGAGGAGC TGATGGGGAG CACGTCTGAC
    AGTTCCTACG GCGACGGCAC CATGGTGGTT CAGAGAACGC CGGAGGAGGA GCGCAGTCTC
    CTTCATGCTG ACAGTAACGG ATATACCAAC CTGCCCGACG TGGTGCAACC GAGCCATTCT
    CCCACCGAGC CCAAGGGACA GAGCACTTCT CCCAGCAAGG AGGGCGGCGG CTCTGATTAC
    CAGTCTCGCG GGTTGGTGAA AGCCCCTCCC AAAACCTCAT TTACAATGTT TGTGGACCTG
    GGACTGTATC AGTCGTCGGG GGGAGGAGGA GACACCATTC CGGTGGCAGC GGCGTACGTG
    AGCGGAGACC CCGCCCGACT GGAGCAGCTG AAATACGAAG CCCGTAAGGG ATCTGTCGTC
    AATGTAAATC CGACCAATAC GAGGCCGCAC AGCGACACGC CGGAGATCCG CAAGTACAAG
    AAGAGATTCA ACTCGGAAAT CCTTTGCGCC GCTCTCTGGG GTGTGAACCT ATTGGTCGGC
    ACTGAGAATG GACTGATGCT ACTGGACCGC AGTGGGCAGG GCAAAGTATA CACCCTAATT
    ACCCGCCGGC GCTTCCAGCA GATGGACGTC CTTGAGGGGC TCAACTTGCT CATTACTATA
    AGTGGTAAGA GGAACAAGCT GCGGGTGTAT TATCTCTCCT GGCTGCGCAA CAAGATCCTT
    CATAACGACC CCGAGGTGGA GAAGAAGCAG GGCTGGAGCA CCGTGGGGGA CATGGACGGC
    TGCGTGCATT ACAAAGTCGT AAAATACGAG CGAATCAAAT TCCTCGTGAT TGCACTGAAA
    AACTCCGTCG AGGTTTACGC CTGGGCGCCC AAACCCTACC ACAAGTTTAT GGCTTTTAAG
    TCGTTTACAG ATATCCCCCA CCGGCCCCAG CTGGTCGACC TAACGGTGGA GGAAGGCCAG
    AGGCTCAAGG TCATCTACGG TTCCTCGTCG GGATTCCACG CCGTGGATGT CGACTCCGGG
    AACAACTATG ATATTTACAT CCCTGTCCAT ATCCAGAGCC AGATTAACCC CCACGCCATC
    ATCTTTCTGC CAAACACGGA CGGCATGGAG ATGTTACTGT GCTACGAGGA CGAGGGGGTC
    TACGTCAACA CCTACGGGCG CATCATTAAA GACGTGGTGT TGCAGTGGGG AGAGATGCCC
    ACGTCTGTCG CCTATATCTG CTCCAATCAG ATCATGGGCT GGGGGGAGAA GGCCATAGAA
    ATCCGCTCGG TGGAGACGGG GCACCTGGAC GGGGTCTTCA TGCACAAGAG AGCTCAGCGG
    CTGAAGTTCC TGTGTGAGCG CAACGACAAG GTGTTCTTTG CCTCGGTGCG TTCGGGCGGG
    AGCAGCCAGG TTTACTTCAT GACCCTGAAT CGCAGCTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa40996
    Clone ID Related Accession (Same CDS sequence) XM_018089849.1
    Accession Version XM_018089849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product misshapen-like kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683461.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCTCAGCG GCCTCCAGAA ATACAGGAGC ACAAGCCACT ACGCATTACT
    TCTCAGAACC GTGCTGCTCT CAGGCCAGCT CCCCGGCCCA ACTCCTTGCA GGACCCAATG
    GCACCCCCTG TCCGTCATGC TCCTGTTCCC CTTCCTGCCA ACCGGAATCA CCTTTCCCGC
    CAGAGCTCTG ACCCATCCCC TGATGGGGTG CCACCCTCTT CCCGTCTAGA GAGGGGACCC
    TGGGTGAAAA AGGAGGCAGA GGTGCACCCT CCCAAGGTTC CCCAGAGAAC AACGTCCATT
    GCCACGGCCC TGAATACGAG TGGTTCTGGG GGCAGCAGGC CGGTGCAGGC TGTGAGAGCC
    AGTAACCCGG ACCTGCGACG GGCAGAGAGC GGCTGGGAGA GAGGAGACTC TTTGCTGCAG
    TCCCCCCACC CTAACCTGCC CCAGGCTGGC TCCCTCGAGA GGAACCGCAT AGCCCCCCAC
    AAGCTGGAGG AATCCTCTAT TAGCCCTCCA GGGAGCAAAA CCCCAGGAGA AGAGCATCGA
    TCCCGCCCTG GGCGCCCAGC AAGCTATAAG AGAGCCATAG GAGAGGATCA CCTCCTGGTG
    AAGGACCGGC CCGAAGATGT GCCCAAACCC CCTAAGAAAG CGTTGGACTA CTCTTCCTCC
    AGCGATGAAC TGGACAGCAG TGATGAAGAG GAAGAGGAGG GGGATGCTGA GAGACAGGAG
    CCCAACAGGG ACAGCTCAGG AGCCAGTCGC AGCGGGCGAG ACGCCGATAC AGACAGCGTC
    AGCACCATGG TGGTTCATGA TGTGGAGGAG CTGATGGGGA GCACGTCTGA CAGTTCCTAC
    GGCGACGGCA CCATGGTGGT TCAGAGAACG CCGGAGGAGG AGCGCAGTCT CCTTCATGCT
    GACAGTAACG GATATACCAA CCTGCCCGAC GTGGTGCAAC CGAGCCATTC TCCCACCGAG
    CCCAAGGGAC AGAGCACTTC TCCCAGCAAG GAGGGCGGCG GCTCTGATTA CCAGTCTCGC
    GGGTTGGTGA AAGCCCCTCC CAAAACCTCA TTTACAATGT TTGTGGACCT GGGACTGTAT
    CAGTCGTCGG GGGGAGGAGG AGACACCATT CCGGTGGCAG CGGCGTACGT GAGCGGAGAC
    CCCGCCCGAC TGGAGCAGCT GAAATACGAA GCCCGTAAGG GATCTGTCGT CAATGTAAAT
    CCGACCAATA CGAGGCCGCA CAGCGACACG CCGGAGATCC GCAAGTACAA GAAGAGATTC
    AACTCGGAAA TCCTTTGCGC CGCTCTCTGG GGTGTGAACC TATTGGTCGG CACTGAGAAT
    GGACTGATGC TACTGGACCG CAGTGGGCAG GGCAAAGTAT ACACCCTAAT TACCCGCCGG
    CGCTTCCAGC AGATGGACGT CCTTGAGGGG CTCAACTTGC TCATTACTAT AAGTGGTAAG
    AGGAACAAGC TGCGGGTGTA TTATCTCTCC TGGCTGCGCA ACAAGATCCT TCATAACGAC
    CCCGAGGTGG AGAAGAAGCA GGGCTGGAGC ACCGTGGGGG ACATGGACGG CTGCGTGCAT
    TACAAAGTCG TAAAATACGA GCGAATCAAA TTCCTCGTGA TTGCACTGAA AAACTCCGTC
    GAGGTTTACG CCTGGGCGCC CAAACCCTAC CACAAGTTTA TGGCTTTTAA GTCGTTTACA
    GATATCCCCC ACCGGCCCCA GCTGGTCGAC CTAACGGTGG AGGAAGGCCA GAGGCTCAAG
    GTCATCTACG GTTCCTCGTC GGGATTCCAC GCCGTGGATG TCGACTCCGG GAACAACTAT
    GATATTTACA TCCCTGTCCA TATCCAGAGC CAGATTAACC CCCACGCCAT CATCTTTCTG
    CCAAACACGG ACGGCATGGA GATGTTACTG TGCTACGAGG ACGAGGGGGT CTACGTCAAC
    ACCTACGGGC GCATCATTAA AGACGTGGTG TTGCAGTGGG GAGAGATGCC CACGTCTGTC
    GCCTATATCT GCTCCAATCA GATCATGGGC TGGGGGGAGA AGGCCATAGA AATCCGCTCG
    GTGGAGACGG GGCACCTGGA CGGGGTCTTC ATGCACAAGA GAGCTCAGCG GCTGAAGTTC
    CTGTGTGAGC GCAACGACAA GGTGTTCTTT GCCTCGGTGC GTTCGGGCGG GAGCAGCCAG
    GTTTACTTCA TGACCCTGAA TCGCAGCTGC ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945338.1
    CDS1358..5182
    Translation

    Target ORF information:

    RefSeq Version XM_018089849.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis misshapen-like kinase 1 (mink1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGTCGG ACCCACCGGC TCGGAGTTTG GATGACATCG ACCTGTCTGC GCTAAGGGAT 
    CCCGCTGGAA TATTTGAACT CGTTGAAGTT GTTGGAAATG GAACATACGG ACAGGTGTAT
    AAGGGCCGCC ATGTCAAGAC TGGGCAGCTG GCCGCTATCA AGGTCATGGA TGTCACAGAG
    GACGAGGAAG AGGAGATTAA ACAGGAGATT AATATGCTGA AGAAGTACTC TCATCATCGC
    AACATTGCAA CCTACTACGG AGCTTTCATC AAGAAGAGCC CGCCGGGCAA TGACGACCAG
    CTGTGGCTGG TCATGGAGTT CTGCGGAGCC GGCTCAGTAA CAGATCTAGT GAAAAACACC
    AAAGGAAATG CCCTGAAGGA AGACTGCATC GCCTACATCT GCAGGGAGAT TCTGCGGGGG
    CTGTCCCACC TACATGCTCA CAAAGTGATA CACAGAGACA TCAAGGGGCA GAATGTCCTT
    CTCACAGAGA ATGCAGAGGT GAAACTCGTG GACTTTGGTG TGAGCGCCCA GCTCGACCGC
    ACAGTGGGGA GAAGAAATAC TTTCATAGGG ACCCCATACT GGATGGCCCC TGAGGTGATT
    GCCTGCGATG AGAATCCAGA TGCCACCTAT GATTACAGGA GTGACATCTG GTCGCTGGGA
    ATCACAGCTA TAGAAATGGC CGAGGGAGCC CCACCACTCT GCGACATGCA CCCAATGAGA
    GCTCTCTTCC TTATCCCCAG GAATCCACCC CCAAAACTCA AGTCCAAAAA GTGGTCCAAA
    AAGTTCATTG ACTTCATTGA CACCTGTCTG ATCAAGAACT ACATGAGCCG GCCACCCACC
    GAGCAGCTGC TGAAGCACCC CTTTATCAAG GACCAGCCGA CGGAGAGGCA GGTCCGGATC
    CAGCTGAAAG ATCACATAGA CAGATCTAGG AAAAAGCGAG ATGAGACAGA GTATGAATAC
    AGTGGCAGTG AGGATGAGGA GGAGAACCAC GGAGAGGAAG GAGAACCAAG TTCCATAATG
    AACGTGCCGG GGGAATCCAC TCTGAGACGC GAGTTCCTGA GGCTGCAGCA GGAGAACAAA
    AGTAACTCAG AAGCCCTAAA GCAGCAGCAG ATAGGCGCCC AGCACCGCGA CTCCGAGGCA
    CATATCAAGC AGCTGCTCCA TGAGAGGCAG AGGCGCATAG AGGAGCAAAA GGAAGAGCGC
    CGGCGAGTGG AAGAGCAACA GCGGCGGGAG AGAGAGCAGA GGAAGCAGCA GGAGAAGGAG
    CAGCAAAGGC GCCGTGATGA CTTACGGCGG GAGGAAGAGA GGAGGATGGC AGAGAGAGAG
    CAGGAATACA AGCGGAAGCA GCTGGAGGAG CAGAGACAGT CAGAGAGACT GCAGAGACAG
    CTTCAGCAGG AACACGCTTA TCTCAAGTCT CTGCAGCAGC AGCAACAGCA ACAACAGAAG
    CAGCAACAGC AGCAGCAGCA GGATAAGAAG CCGCTGTACC ATTACAACCG GGGGGTAATG
    AACCCCAGTG AGAAGCCGGC CTGGGCTAGA GAGGTGGAGG AGCGTTCGAG GCTCAACAAG
    CAGAGTTCCC CCCTCTCCAT TACTAAGCTC AGCTCGGTGG AGTCTACTGG AAACCCGTCG
    CAAACCCCAC CCTCTCAGCG GCCTCCAGAA ATACAGGAGC ACAAGCCACT ACGCATTACT
    TCTCAGAACC GTGCTGCTCT CAGGCCAGCT CCCCGGCCCA ACTCCTTGCA GGACCCAATG
    GCACCCCCTG TCCGTCATGC TCCTGTTCCC CTTCCTGCCA ACCGGAATCA CCTTTCCCGC
    CAGAGCTCTG ACCCATCCCC TGATGGGGTG CCACCCTCTT CCCGTCTAGA GAGGGGACCC
    TGGGTGAAAA AGGAGGCAGA GGTGCACCCT CCCAAGGTTC CCCAGAGAAC AACGTCCATT
    GCCACGGCCC TGAATACGAG TGGTTCTGGG GGCAGCAGGC CGGTGCAGGC TGTGAGAGCC
    AGTAACCCGG ACCTGCGACG GGCAGAGAGC GGCTGGGAGA GAGGAGACTC TTTGCTGCAG
    TCCCCCCACC CTAACCTGCC CCAGGCTGGC TCCCTCGAGA GGAACCGCAT AGCCCCCCAC
    AAGCTGGAGG AATCCTCTAT TAGCCCTCCA GGGAGCAAAA CCCCAGGAGA AGAGCATCGA
    TCCCGCCCTG GGCGCCCAGC AAGCTATAAG AGAGCCATAG GAGAGGATCA CCTCCTGGTG
    AAGGACCGGC CCGAAGATGT GCCCAAACCC CCTAAGAAAG CGTTGGACTA CTCTTCCTCC
    AGCGATGAAC TGGACAGCAG TGATGAAGAG GAAGAGGAGG GGGATGCTGA GAGACAGGAG
    CCCAACAGGG ACAGCTCAGG AGCCAGTCGC AGCGGGCGAG ACGCCGATAC AGACAGCGTC
    AGCACCATGG TGGTTCATGA TGTGGAGGAG CTGATGGGGA GCACGTCTGA CAGTTCCTAC
    GGCGACGGCA CCATGGTGGT TCAGAGAACG CCGGAGGAGG AGCGCAGTCT CCTTCATGCT
    GACAGTAACG GATATACCAA CCTGCCCGAC GTGGTGCAAC CGAGCCATTC TCCCACCGAG
    CCCAAGGGAC AGAGCACTTC TCCCAGCAAG GAGGGCGGCG GCTCTGATTA CCAGTCTCGC
    GGGTTGGTGA AAGCCCCTCC CAAAACCTCA TTTACAATGT TTGTGGACCT GGGACTGTAT
    CAGTCGTCGG GGGGAGGAGG AGACACCATT CCGGTGGCAG CGGCGTACGT GAGCGGAGAC
    CCCGCCCGAC TGGAGCAGCT GAAATACGAA GCCCGTAAGG GATCTGTCGT CAATGTAAAT
    CCGACCAATA CGAGGCCGCA CAGCGACACG CCGGAGATCC GCAAGTACAA GAAGAGATTC
    AACTCGGAAA TCCTTTGCGC CGCTCTCTGG GGTGTGAACC TATTGGTCGG CACTGAGAAT
    GGACTGATGC TACTGGACCG CAGTGGGCAG GGCAAAGTAT ACACCCTAAT TACCCGCCGG
    CGCTTCCAGC AGATGGACGT CCTTGAGGGG CTCAACTTGC TCATTACTAT AAGTGGTAAG
    AGGAACAAGC TGCGGGTGTA TTATCTCTCC TGGCTGCGCA ACAAGATCCT TCATAACGAC
    CCCGAGGTGG AGAAGAAGCA GGGCTGGAGC ACCGTGGGGG ACATGGACGG CTGCGTGCAT
    TACAAAGTCG TAAAATACGA GCGAATCAAA TTCCTCGTGA TTGCACTGAA AAACTCCGTC
    GAGGTTTACG CCTGGGCGCC CAAACCCTAC CACAAGTTTA TGGCTTTTAA GTCGTTTACA
    GATATCCCCC ACCGGCCCCA GCTGGTCGAC CTAACGGTGG AGGAAGGCCA GAGGCTCAAG
    GTCATCTACG GTTCCTCGTC GGGATTCCAC GCCGTGGATG TCGACTCCGG GAACAACTAT
    GATATTTACA TCCCTGTCCA TATCCAGAGC CAGATTAACC CCCACGCCAT CATCTTTCTG
    CCAAACACGG ACGGCATGGA GATGTTACTG TGCTACGAGG ACGAGGGGGT CTACGTCAAC
    ACCTACGGGC GCATCATTAA AGACGTGGTG TTGCAGTGGG GAGAGATGCC CACGTCTGTC
    GCCTATATCT GCTCCAATCA GATCATGGGC TGGGGGGAGA AGGCCATAGA AATCCGCTCG
    GTGGAGACGG GGCACCTGGA CGGGGTCTTC ATGCACAAGA GAGCTCAGCG GCTGAAGTTC
    CTGTGTGAGC GCAACGACAA GGTGTTCTTT GCCTCGGTGC GTTCGGGCGG GAGCAGCCAG
    GTTTACTTCA TGACCCTGAA TCGCAGCTGC ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.