Mink1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006533681.3	XP_006533744.1	misshapen-like kinase 1 isoform X4
XM_017314645.2	XP_017170134.1	misshapen-like kinase 1 isoform X6
XM_030246118.1	XP_030101978.1	misshapen-like kinase 1 isoform X9
XM_006533677.2	XP_006533740.1	misshapen-like kinase 1 isoform X1
XM_006533682.3	XP_006533745.1	misshapen-like kinase 1 isoform X5
XM_006533679.3	XP_006533742.1	misshapen-like kinase 1 isoform X3
XM_006533681.4	XP_006533744.1	misshapen-like kinase 1 isoform X4
XM_017314646.2	XP_017170135.1	misshapen-like kinase 1 isoform X7
XM_006533678.2	XP_006533741.1	misshapen-like kinase 1 isoform X2
XM_006533679.2	XP_006533742.1	misshapen-like kinase 1 isoform X3
XM_017314645.1	XP_017170134.1	misshapen-like kinase 1 isoform X6
NM_001045959.1	NP_001039424.1	misshapen-like kinase 1 isoform 3
XM_006533677.3	XP_006533740.1	misshapen-like kinase 1 isoform X1
NM_176893.2	NP_795712.2	misshapen-like kinase 1 isoform 2
XM_006533678.3	XP_006533741.1	misshapen-like kinase 1 isoform X2
XM_006533682.4	XP_006533745.1	misshapen-like kinase 1 isoform X5
XM_030246117.1	XP_030101977.1	misshapen-like kinase 1 isoform X8
NM_016713.2	NP_057922.2	misshapen-like kinase 1 isoform 1
XM_017314646.1	XP_017170135.1	misshapen-like kinase 1 isoform X7
NM_001045964.1	NP_001039429.1	misshapen-like kinase 1 isoform 4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu46565	XM_006533677.3 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu46566	XM_006533678.3 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu46567	XM_006533679.3 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu46569	XM_006533681.4 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu46570	XM_006533682.4 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77971	XM_017314645.2 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu77972	XM_017314646.2 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85833	XM_030246117.1 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85834	XM_030246118.1 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu13990	NM_176893.2 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$769.30 $1099.00
OMu14018	NM_001045964.1 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$790.30 $1129.00
OMu14043	NM_016713.2 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$762.30 $1089.00
OMu14135	NM_001045959.1 Latest version!	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$790.30 $1129.00
View Old Accession Versions >>
OMu46569	XM_006533681.3	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu46567	XM_006533679.2	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu77971	XM_017314645.1	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu46565	XM_006533677.2	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu46566	XM_006533678.2	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu46570	XM_006533682.3	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu77972	XM_017314646.1	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

Mink1 ( XM_006533677.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533740.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu46565
Clone ID Related Accession (Same CDS sequence)	XM_006533677.2 , XM_006533677.3
Accession Version	XM_006533677.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG CAGTGATGGT 
GATACAGACA GCGTCAGCAC CATGGTGGTT CATGATGTTG AGGAGATATC CGGGACCCAG 
CCCTCATATG GCGGCGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA ACGAAGCCTG 
CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCTGATG TGGTCCAGCC CAGCCACTCA 
CCTACTGAGA ACAGCAAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCAG TGATTACCAG 
TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGCTCATTCA CCATGTTTGT GGATCTAGGG 
ATCTACCAGC CTGGAGGCAG TGGGGACACC ATCCCTATCA CAGCCCTAGT GGGTGGAGAA 
GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT 
CCCACCAACA CCCGAGCTCA TAGTGAAACT CCTGAAATTC GCAAGTACAA GAAGCGATTC 
AACTCAGAGA TCCTATGTGC AGCTCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT 
GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAGGTGT ATGGACTTAT TGGGCGACGA 
CGCTTCCAGC AAATGGATGT CTTAGAAGGG CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ACAAGATCCT ACACAATGAC 
CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC 
TACCGTGTTG TGAAATATGA ACGGATTAAG TTCCTGGTCA TTGCCCTGAA GAACTCCGTG 
GAGGTTTATG CCTGGGCTCC CAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT 
GACCTCCCTC ACCGCCCTCT ACTGGTGGAC CTGACAGTAG AGGAGGGACA GCGGCTCAAG 
GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGATTCTGG GAACAGCTAT 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACTG ATGGCATGGA GATGCTGCTG TGCTATGAAG ATGAGGGTGT CTATGTCAAC 
ACTTACGGGC GGATCATCAA GGATGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT 
GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GCAATGACAA GGTGTTTTTT GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA 
GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533740.1
CDS	1491..5615
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533677.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533677.3

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG CAGTGATGGT 
GATACAGACA GCGTCAGCAC CATGGTGGTT CATGATGTTG AGGAGATATC CGGGACCCAG 
CCCTCATATG GCGGCGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA ACGAAGCCTG 
CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCTGATG TGGTCCAGCC CAGCCACTCA 
CCTACTGAGA ACAGCAAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCAG TGATTACCAG 
TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGCTCATTCA CCATGTTTGT GGATCTAGGG 
ATCTACCAGC CTGGAGGCAG TGGGGACACC ATCCCTATCA CAGCCCTAGT GGGTGGAGAA 
GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT 
CCCACCAACA CCCGAGCTCA TAGTGAAACT CCTGAAATTC GCAAGTACAA GAAGCGATTC 
AACTCAGAGA TCCTATGTGC AGCTCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT 
GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAGGTGT ATGGACTTAT TGGGCGACGA 
CGCTTCCAGC AAATGGATGT CTTAGAAGGG CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ACAAGATCCT ACACAATGAC 
CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC 
TACCGTGTTG TGAAATATGA ACGGATTAAG TTCCTGGTCA TTGCCCTGAA GAACTCCGTG 
GAGGTTTATG CCTGGGCTCC CAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT 
GACCTCCCTC ACCGCCCTCT ACTGGTGGAC CTGACAGTAG AGGAGGGACA GCGGCTCAAG 
GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGATTCTGG GAACAGCTAT 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACTG ATGGCATGGA GATGCTGCTG TGCTATGAAG ATGAGGGTGT CTATGTCAAC 
ACTTACGGGC GGATCATCAA GGATGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT 
GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GCAATGACAA GGTGTTTTTT GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA 
GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533678.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533741.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46566
Clone ID Related Accession (Same CDS sequence)	XM_006533678.2 , XM_006533678.3
Accession Version	XM_006533678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG TGATGGTGAT 
ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTTGAGG AGATATCCGG GACCCAGCCC 
TCATATGGCG GCGGCACCAT GGTGGTCCAG CGTACTCCTG AAGAGGAACG AAGCCTGCTG 
CTTGCTGATA GCAATGGCTA CACAAACCTG CCTGATGTGG TCCAGCCCAG CCACTCACCT 
ACTGAGAACA GCAAAGGTCA AAGCCCTCCA ACAAAGGATG GAGGCAGTGA TTACCAGTCT 
CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC TCATTCACCA TGTTTGTGGA TCTAGGGATC 
TACCAGCCTG GAGGCAGTGG GGACACCATC CCTATCACAG CCCTAGTGGG TGGAGAAGGT 
GGTCGCCTTG ATCAACTGCA GTTCGATGTG AGGAAGGGCT CTGTGGTCAA CGTCAATCCC 
ACCAACACCC GAGCTCATAG TGAAACTCCT GAAATTCGCA AGTACAAGAA GCGATTCAAC 
TCAGAGATCC TATGTGCAGC TCTCTGGGGG GTCAACCTCC TAGTGGGCAC AGAGAATGGG 
CTGATGTTGC TGGACCGAAG TGGGCAGGGC AAGGTGTATG GACTTATTGG GCGACGACGC 
TTCCAGCAAA TGGATGTCTT AGAAGGGCTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTATATTA CCTGTCCTGG CTTCGGAACA AGATCCTACA CAATGACCCA 
GAGGTGGAAA AGAAGCAGGG GTGGACCACC GTGGGGGACA TGGAGGGCTG CGGCCACTAC 
CGTGTTGTGA AATATGAACG GATTAAGTTC CTGGTCATTG CCCTGAAGAA CTCCGTGGAG 
GTTTATGCCT GGGCTCCCAA ACCCTACCAC AAATTCATGG CCTTCAAGTC CTTTGCTGAC 
CTCCCTCACC GCCCTCTACT GGTGGACCTG ACAGTAGAGG AGGGACAGCG GCTCAAGGTC 
ATCTATGGCT CCAGTGCTGG CTTCCATGCT GTGGATGTTG ATTCTGGGAA CAGCTATGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACTGATG GCATGGAGAT GCTGCTGTGC TATGAAGATG AGGGTGTCTA TGTCAACACT 
TACGGGCGGA TCATCAAGGA TGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT AATGGGCTGG GGTGAGAAGG CCATAGAGAT CCGCTCTGTG 
GAGACAGGCC ACCTAGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGCA ATGACAAGGT GTTTTTTGCC TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT 
TACTTTATGA CTCTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533741.1
CDS	1491..5612
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533678.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533678.3

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG TGATGGTGAT 
ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTTGAGG AGATATCCGG GACCCAGCCC 
TCATATGGCG GCGGCACCAT GGTGGTCCAG CGTACTCCTG AAGAGGAACG AAGCCTGCTG 
CTTGCTGATA GCAATGGCTA CACAAACCTG CCTGATGTGG TCCAGCCCAG CCACTCACCT 
ACTGAGAACA GCAAAGGTCA AAGCCCTCCA ACAAAGGATG GAGGCAGTGA TTACCAGTCT 
CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC TCATTCACCA TGTTTGTGGA TCTAGGGATC 
TACCAGCCTG GAGGCAGTGG GGACACCATC CCTATCACAG CCCTAGTGGG TGGAGAAGGT 
GGTCGCCTTG ATCAACTGCA GTTCGATGTG AGGAAGGGCT CTGTGGTCAA CGTCAATCCC 
ACCAACACCC GAGCTCATAG TGAAACTCCT GAAATTCGCA AGTACAAGAA GCGATTCAAC 
TCAGAGATCC TATGTGCAGC TCTCTGGGGG GTCAACCTCC TAGTGGGCAC AGAGAATGGG 
CTGATGTTGC TGGACCGAAG TGGGCAGGGC AAGGTGTATG GACTTATTGG GCGACGACGC 
TTCCAGCAAA TGGATGTCTT AGAAGGGCTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTATATTA CCTGTCCTGG CTTCGGAACA AGATCCTACA CAATGACCCA 
GAGGTGGAAA AGAAGCAGGG GTGGACCACC GTGGGGGACA TGGAGGGCTG CGGCCACTAC 
CGTGTTGTGA AATATGAACG GATTAAGTTC CTGGTCATTG CCCTGAAGAA CTCCGTGGAG 
GTTTATGCCT GGGCTCCCAA ACCCTACCAC AAATTCATGG CCTTCAAGTC CTTTGCTGAC 
CTCCCTCACC GCCCTCTACT GGTGGACCTG ACAGTAGAGG AGGGACAGCG GCTCAAGGTC 
ATCTATGGCT CCAGTGCTGG CTTCCATGCT GTGGATGTTG ATTCTGGGAA CAGCTATGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACTGATG GCATGGAGAT GCTGCTGTGC TATGAAGATG AGGGTGTCTA TGTCAACACT 
TACGGGCGGA TCATCAAGGA TGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT AATGGGCTGG GGTGAGAAGG CCATAGAGAT CCGCTCTGTG 
GAGACAGGCC ACCTAGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGCA ATGACAAGGT GTTTTTTGCC TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT 
TACTTTATGA CTCTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533679.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533742.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.

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CloneID	OMu46567
Clone ID Related Accession (Same CDS sequence)	XM_006533679.3 , XM_006533679.2
Accession Version	XM_006533679.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTCAAA 
GAGCGGACTC TGGATGAGGC CCCTAAGCCT CCCAAGAAGG CCATGGACTA CTCCTCATCC 
AGTGAGGAGG TGGAAAGCAG TGAAGAGGAG GAGGAGGAAG GCGATGGGGA GCCGTCAGAG 
GGGAGCAGAG ACACTCCCGG GGGCCGCAGT GATGGTGATA CAGACAGCGT CAGCACCATG 
GTGGTTCATG ATGTTGAGGA GATATCCGGG ACCCAGCCCT CATATGGCGG CGGCACCATG 
GTGGTCCAGC GTACTCCTGA AGAGGAACGA AGCCTGCTGC TTGCTGATAG CAATGGCTAC 
ACAAACCTGC CTGATGTGGT CCAGCCCAGC CACTCACCTA CTGAGAACAG CAAAGGTCAA 
AGCCCTCCAA CAAAGGATGG AGGCAGTGAT TACCAGTCTC GTGGGCTGGT AAAGGCCCCA 
GGAAAGAGCT CATTCACCAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG 
GACACCATCC CTATCACAGC CCTAGTGGGT GGAGAAGGTG GTCGCCTTGA TCAACTGCAG 
TTCGATGTGA GGAAGGGCTC TGTGGTCAAC GTCAATCCCA CCAACACCCG AGCTCATAGT 
GAAACTCCTG AAATTCGCAA GTACAAGAAG CGATTCAACT CAGAGATCCT ATGTGCAGCT 
CTCTGGGGGG TCAACCTCCT AGTGGGCACA GAGAATGGGC TGATGTTGCT GGACCGAAGT 
GGGCAGGGCA AGGTGTATGG ACTTATTGGG CGACGACGCT TCCAGCAAAT GGATGTCTTA 
GAAGGGCTCA ACTTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTATATTAC 
CTGTCCTGGC TTCGGAACAA GATCCTACAC AATGACCCAG AGGTGGAAAA GAAGCAGGGG 
TGGACCACCG TGGGGGACAT GGAGGGCTGC GGCCACTACC GTGTTGTGAA ATATGAACGG 
ATTAAGTTCC TGGTCATTGC CCTGAAGAAC TCCGTGGAGG TTTATGCCTG GGCTCCCAAA 
CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCTGACC TCCCTCACCG CCCTCTACTG 
GTGGACCTGA CAGTAGAGGA GGGACAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC 
TTCCATGCTG TGGATGTTGA TTCTGGGAAC AGCTATGACA TCTACATCCC TGTACATATC 
CAGAGCCAGA TCACACCCCA CGCCATCATC TTCCTCCCCA ACACTGATGG CATGGAGATG 
CTGCTGTGCT ATGAAGATGA GGGTGTCTAT GTCAACACTT ACGGGCGGAT CATCAAGGAT 
GTGGTGCTGC AGTGGGGAGA GATGCCCACC TCTGTGGCCT ACATCTGCTC CAACCAGATA 
ATGGGCTGGG GTGAGAAGGC CATAGAGATC CGCTCTGTGG AGACAGGCCA CCTAGATGGG 
GTCTTCATGC ACAAACGAGC CCAGAGGCTC AAGTTCCTGT GTGAGCGCAA TGACAAGGTG 
TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC AGCCAAGTTT ACTTTATGAC TCTGAACCGT 
AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533742.1
CDS	1491..5591
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533679.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533679.3

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTCAAA 
GAGCGGACTC TGGATGAGGC CCCTAAGCCT CCCAAGAAGG CCATGGACTA CTCCTCATCC 
AGTGAGGAGG TGGAAAGCAG TGAAGAGGAG GAGGAGGAAG GCGATGGGGA GCCGTCAGAG 
GGGAGCAGAG ACACTCCCGG GGGCCGCAGT GATGGTGATA CAGACAGCGT CAGCACCATG 
GTGGTTCATG ATGTTGAGGA GATATCCGGG ACCCAGCCCT CATATGGCGG CGGCACCATG 
GTGGTCCAGC GTACTCCTGA AGAGGAACGA AGCCTGCTGC TTGCTGATAG CAATGGCTAC 
ACAAACCTGC CTGATGTGGT CCAGCCCAGC CACTCACCTA CTGAGAACAG CAAAGGTCAA 
AGCCCTCCAA CAAAGGATGG AGGCAGTGAT TACCAGTCTC GTGGGCTGGT AAAGGCCCCA 
GGAAAGAGCT CATTCACCAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG 
GACACCATCC CTATCACAGC CCTAGTGGGT GGAGAAGGTG GTCGCCTTGA TCAACTGCAG 
TTCGATGTGA GGAAGGGCTC TGTGGTCAAC GTCAATCCCA CCAACACCCG AGCTCATAGT 
GAAACTCCTG AAATTCGCAA GTACAAGAAG CGATTCAACT CAGAGATCCT ATGTGCAGCT 
CTCTGGGGGG TCAACCTCCT AGTGGGCACA GAGAATGGGC TGATGTTGCT GGACCGAAGT 
GGGCAGGGCA AGGTGTATGG ACTTATTGGG CGACGACGCT TCCAGCAAAT GGATGTCTTA 
GAAGGGCTCA ACTTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTATATTAC 
CTGTCCTGGC TTCGGAACAA GATCCTACAC AATGACCCAG AGGTGGAAAA GAAGCAGGGG 
TGGACCACCG TGGGGGACAT GGAGGGCTGC GGCCACTACC GTGTTGTGAA ATATGAACGG 
ATTAAGTTCC TGGTCATTGC CCTGAAGAAC TCCGTGGAGG TTTATGCCTG GGCTCCCAAA 
CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCTGACC TCCCTCACCG CCCTCTACTG 
GTGGACCTGA CAGTAGAGGA GGGACAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC 
TTCCATGCTG TGGATGTTGA TTCTGGGAAC AGCTATGACA TCTACATCCC TGTACATATC 
CAGAGCCAGA TCACACCCCA CGCCATCATC TTCCTCCCCA ACACTGATGG CATGGAGATG 
CTGCTGTGCT ATGAAGATGA GGGTGTCTAT GTCAACACTT ACGGGCGGAT CATCAAGGAT 
GTGGTGCTGC AGTGGGGAGA GATGCCCACC TCTGTGGCCT ACATCTGCTC CAACCAGATA 
ATGGGCTGGG GTGAGAAGGC CATAGAGATC CGCTCTGTGG AGACAGGCCA CCTAGATGGG 
GTCTTCATGC ACAAACGAGC CCAGAGGCTC AAGTTCCTGT GTGAGCGCAA TGACAAGGTG 
TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC AGCCAAGTTT ACTTTATGAC TCTGAACCGT 
AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533681.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533744.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.

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CloneID	OMu46569
Clone ID Related Accession (Same CDS sequence)	XM_006533681.3 , XM_006533681.4
Accession Version	XM_006533681.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533681.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGCAGTGAT GGTGATACAG ACAGCGTCAG CACCATGGTG 
GTTCATGATG TTGAGGAGAT ATCCGGGACC CAGCCCTCAT ATGGCGGCGG CACCATGGTG 
GTCCAGCGTA CTCCTGAAGA GGAACGAAGC CTGCTGCTTG CTGATAGCAA TGGCTACACA 
AACCTGCCTG ATGTGGTCCA GCCCAGCCAC TCACCTACTG AGAACAGCAA AGGTCAAAGC 
CCTCCAACAA AGGATGGAGG CAGTGATTAC CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA 
AAGAGCTCAT TCACCATGTT TGTGGATCTA GGGATCTACC AGCCTGGAGG CAGTGGGGAC 
ACCATCCCTA TCACAGCCCT AGTGGGTGGA GAAGGTGGTC GCCTTGATCA ACTGCAGTTC 
GATGTGAGGA AGGGCTCTGT GGTCAACGTC AATCCCACCA ACACCCGAGC TCATAGTGAA 
ACTCCTGAAA TTCGCAAGTA CAAGAAGCGA TTCAACTCAG AGATCCTATG TGCAGCTCTC 
TGGGGGGTCA ACCTCCTAGT GGGCACAGAG AATGGGCTGA TGTTGCTGGA CCGAAGTGGG 
CAGGGCAAGG TGTATGGACT TATTGGGCGA CGACGCTTCC AGCAAATGGA TGTCTTAGAA 
GGGCTCAACT TGCTCATCAC CATCTCAGGG AAAAGGAACA AACTGCGGGT ATATTACCTG 
TCCTGGCTTC GGAACAAGAT CCTACACAAT GACCCAGAGG TGGAAAAGAA GCAGGGGTGG 
ACCACCGTGG GGGACATGGA GGGCTGCGGC CACTACCGTG TTGTGAAATA TGAACGGATT 
AAGTTCCTGG TCATTGCCCT GAAGAACTCC GTGGAGGTTT ATGCCTGGGC TCCCAAACCC 
TACCACAAAT TCATGGCCTT CAAGTCCTTT GCTGACCTCC CTCACCGCCC TCTACTGGTG 
GACCTGACAG TAGAGGAGGG ACAGCGGCTC AAGGTCATCT ATGGCTCCAG TGCTGGCTTC 
CATGCTGTGG ATGTTGATTC TGGGAACAGC TATGACATCT ACATCCCTGT ACATATCCAG 
AGCCAGATCA CACCCCACGC CATCATCTTC CTCCCCAACA CTGATGGCAT GGAGATGCTG 
CTGTGCTATG AAGATGAGGG TGTCTATGTC AACACTTACG GGCGGATCAT CAAGGATGTG 
GTGCTGCAGT GGGGAGAGAT GCCCACCTCT GTGGCCTACA TCTGCTCCAA CCAGATAATG 
GGCTGGGGTG AGAAGGCCAT AGAGATCCGC TCTGTGGAGA CAGGCCACCT AGATGGGGTC 
TTCATGCACA AACGAGCCCA GAGGCTCAAG TTCCTGTGTG AGCGCAATGA CAAGGTGTTT 
TTTGCCTCTG TCCGCTCTGG AGGAAGCAGC CAAGTTTACT TTATGACTCT GAACCGTAAC 
TGCATCATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533744.1
CDS	1491..5528
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533681.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533681.4

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGCAGTGAT GGTGATACAG ACAGCGTCAG CACCATGGTG 
GTTCATGATG TTGAGGAGAT ATCCGGGACC CAGCCCTCAT ATGGCGGCGG CACCATGGTG 
GTCCAGCGTA CTCCTGAAGA GGAACGAAGC CTGCTGCTTG CTGATAGCAA TGGCTACACA 
AACCTGCCTG ATGTGGTCCA GCCCAGCCAC TCACCTACTG AGAACAGCAA AGGTCAAAGC 
CCTCCAACAA AGGATGGAGG CAGTGATTAC CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA 
AAGAGCTCAT TCACCATGTT TGTGGATCTA GGGATCTACC AGCCTGGAGG CAGTGGGGAC 
ACCATCCCTA TCACAGCCCT AGTGGGTGGA GAAGGTGGTC GCCTTGATCA ACTGCAGTTC 
GATGTGAGGA AGGGCTCTGT GGTCAACGTC AATCCCACCA ACACCCGAGC TCATAGTGAA 
ACTCCTGAAA TTCGCAAGTA CAAGAAGCGA TTCAACTCAG AGATCCTATG TGCAGCTCTC 
TGGGGGGTCA ACCTCCTAGT GGGCACAGAG AATGGGCTGA TGTTGCTGGA CCGAAGTGGG 
CAGGGCAAGG TGTATGGACT TATTGGGCGA CGACGCTTCC AGCAAATGGA TGTCTTAGAA 
GGGCTCAACT TGCTCATCAC CATCTCAGGG AAAAGGAACA AACTGCGGGT ATATTACCTG 
TCCTGGCTTC GGAACAAGAT CCTACACAAT GACCCAGAGG TGGAAAAGAA GCAGGGGTGG 
ACCACCGTGG GGGACATGGA GGGCTGCGGC CACTACCGTG TTGTGAAATA TGAACGGATT 
AAGTTCCTGG TCATTGCCCT GAAGAACTCC GTGGAGGTTT ATGCCTGGGC TCCCAAACCC 
TACCACAAAT TCATGGCCTT CAAGTCCTTT GCTGACCTCC CTCACCGCCC TCTACTGGTG 
GACCTGACAG TAGAGGAGGG ACAGCGGCTC AAGGTCATCT ATGGCTCCAG TGCTGGCTTC 
CATGCTGTGG ATGTTGATTC TGGGAACAGC TATGACATCT ACATCCCTGT ACATATCCAG 
AGCCAGATCA CACCCCACGC CATCATCTTC CTCCCCAACA CTGATGGCAT GGAGATGCTG 
CTGTGCTATG AAGATGAGGG TGTCTATGTC AACACTTACG GGCGGATCAT CAAGGATGTG 
GTGCTGCAGT GGGGAGAGAT GCCCACCTCT GTGGCCTACA TCTGCTCCAA CCAGATAATG 
GGCTGGGGTG AGAAGGCCAT AGAGATCCGC TCTGTGGAGA CAGGCCACCT AGATGGGGTC 
TTCATGCACA AACGAGCCCA GAGGCTCAAG TTCCTGTGTG AGCGCAATGA CAAGGTGTTT 
TTTGCCTCTG TCCGCTCTGG AGGAAGCAGC CAAGTTTACT TTATGACTCT GAACCGTAAC 
TGCATCATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533682.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533745.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.

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CloneID	OMu46570
Clone ID Related Accession (Same CDS sequence)	XM_006533682.3 , XM_006533682.4
Accession Version	XM_006533682.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006533682.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533745.1
CDS	1491..5504
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533682.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533682.4

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_017314645.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170134.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.

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CloneID	OMu77971
Clone ID Related Accession (Same CDS sequence)	XM_017314645.2 , XM_017314645.1
Accession Version	XM_017314645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGT 
GATGGTGATA CAGACAGCGT CAGCACCATG GTGGTTCATG ATGTTGAGGA GATATCCGGG 
ACCCAGCCCT CATATGGCGG CGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAACGA 
AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CTGATGTGGT CCAGCCCAGC 
CACTCACCTA CTGAGAACAG CAAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCAGTGAT 
TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGCT CATTCACCAT GTTTGTGGAT 
CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GACACCATCC CTATCACAGC CCTAGTGGGT 
GGAGAAGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC 
GTCAATCCCA CCAACACCCG AGCTCATAGT GAAACTCCTG AAATTCGCAA GTACAAGAAG 
CGATTCAACT CAGAGATCCT ATGTGCAGCT CTCTGGGGGG TCAACCTCCT AGTGGGCACA 
GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AGGTGTATGG ACTTATTGGG 
CGACGACGCT TCCAGCAAAT GGATGTCTTA GAAGGGCTCA ACTTGCTCAT CACCATCTCA 
GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAACAA GATCCTACAC 
AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC 
GGCCACTACC GTGTTGTGAA ATATGAACGG ATTAAGTTCC TGGTCATTGC CCTGAAGAAC 
TCCGTGGAGG TTTATGCCTG GGCTCCCAAA CCCTACCACA AATTCATGGC CTTCAAGTCC 
TTTGCTGACC TCCCTCACCG CCCTCTACTG GTGGACCTGA CAGTAGAGGA GGGACAGCGG 
CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA TTCTGGGAAC 
AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAGA TCACACCCCA CGCCATCATC 
TTCCTCCCCA ACACTGATGG CATGGAGATG CTGCTGTGCT ATGAAGATGA GGGTGTCTAT 
GTCAACACTT ACGGGCGGAT CATCAAGGAT GTGGTGCTGC AGTGGGGAGA GATGCCCACC 
TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC 
CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC 
AAGTTCCTGT GTGAGCGCAA TGACAAGGTG TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC 
AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170134.1
CDS	1491..5501
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017314645.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314645.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGT 
GATGGTGATA CAGACAGCGT CAGCACCATG GTGGTTCATG ATGTTGAGGA GATATCCGGG 
ACCCAGCCCT CATATGGCGG CGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAACGA 
AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CTGATGTGGT CCAGCCCAGC 
CACTCACCTA CTGAGAACAG CAAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCAGTGAT 
TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGCT CATTCACCAT GTTTGTGGAT 
CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GACACCATCC CTATCACAGC CCTAGTGGGT 
GGAGAAGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC 
GTCAATCCCA CCAACACCCG AGCTCATAGT GAAACTCCTG AAATTCGCAA GTACAAGAAG 
CGATTCAACT CAGAGATCCT ATGTGCAGCT CTCTGGGGGG TCAACCTCCT AGTGGGCACA 
GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AGGTGTATGG ACTTATTGGG 
CGACGACGCT TCCAGCAAAT GGATGTCTTA GAAGGGCTCA ACTTGCTCAT CACCATCTCA 
GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAACAA GATCCTACAC 
AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC 
GGCCACTACC GTGTTGTGAA ATATGAACGG ATTAAGTTCC TGGTCATTGC CCTGAAGAAC 
TCCGTGGAGG TTTATGCCTG GGCTCCCAAA CCCTACCACA AATTCATGGC CTTCAAGTCC 
TTTGCTGACC TCCCTCACCG CCCTCTACTG GTGGACCTGA CAGTAGAGGA GGGACAGCGG 
CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA TTCTGGGAAC 
AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAGA TCACACCCCA CGCCATCATC 
TTCCTCCCCA ACACTGATGG CATGGAGATG CTGCTGTGCT ATGAAGATGA GGGTGTCTAT 
GTCAACACTT ACGGGCGGAT CATCAAGGAT GTGGTGCTGC AGTGGGGAGA GATGCCCACC 
TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC 
CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC 
AAGTTCCTGT GTGAGCGCAA TGACAAGGTG TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC 
AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_017314646.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170135.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.

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CloneID	OMu77972
Clone ID Related Accession (Same CDS sequence)	XM_017314646.2 , XM_017314646.1
Accession Version	XM_017314646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AGACTTTGTG 
TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAC 
TCCTCATCCA GTGAGGAGGT GGAAAGCAGT GAAGAGGAGG AGGAGGAAGG CGATGGGGAG 
CCGTCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGATAC AGACAGCGTC 
AGCACCATGG TGGTTCATGA TGTTGAGGAG ATATCCGGGA CCCAGCCCTC ATATGGCGGC 
GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAACGAA GCCTGCTGCT TGCTGATAGC 
AATGGCTACA CAAACCTGCC TGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC 
AAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCAGTGATT ACCAGTCTCG TGGGCTGGTA 
AAGGCCCCAG GAAAGAGCTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA 
GGCAGTGGGG ACACCATCCC TATCACAGCC CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT 
CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACCCGA 
GCTCATAGTG AAACTCCTGA AATTCGCAAG TACAAGAAGC GATTCAACTC AGAGATCCTA 
TGTGCAGCTC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG 
GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG 
GATGTCTTAG AAGGGCTCAA CTTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG 
GTATATTACC TGTCCTGGCT TCGGAACAAG ATCCTACACA ATGACCCAGA GGTGGAAAAG 
AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTACCG TGTTGTGAAA 
TATGAACGGA TTAAGTTCCT GGTCATTGCC CTGAAGAACT CCGTGGAGGT TTATGCCTGG 
GCTCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC 
CCTCTACTGG TGGACCTGAC AGTAGAGGAG GGACAGCGGC TCAAGGTCAT CTATGGCTCC 
AGTGCTGGCT TCCATGCTGT GGATGTTGAT TCTGGGAACA GCTATGACAT CTACATCCCT 
GTACATATCC AGAGCCAGAT CACACCCCAC GCCATCATCT TCCTCCCCAA CACTGATGGC 
ATGGAGATGC TGCTGTGCTA TGAAGATGAG GGTGTCTATG TCAACACTTA CGGGCGGATC 
ATCAAGGATG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTGGCCTA CATCTGCTCC 
AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC 
CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT 
GACAAGGTGT TTTTTGCCTC TGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT 
CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170135.1
CDS	1491..5480
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017314646.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314646.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AGACTTTGTG 
TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAC 
TCCTCATCCA GTGAGGAGGT GGAAAGCAGT GAAGAGGAGG AGGAGGAAGG CGATGGGGAG 
CCGTCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGATAC AGACAGCGTC 
AGCACCATGG TGGTTCATGA TGTTGAGGAG ATATCCGGGA CCCAGCCCTC ATATGGCGGC 
GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAACGAA GCCTGCTGCT TGCTGATAGC 
AATGGCTACA CAAACCTGCC TGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC 
AAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCAGTGATT ACCAGTCTCG TGGGCTGGTA 
AAGGCCCCAG GAAAGAGCTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA 
GGCAGTGGGG ACACCATCCC TATCACAGCC CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT 
CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACCCGA 
GCTCATAGTG AAACTCCTGA AATTCGCAAG TACAAGAAGC GATTCAACTC AGAGATCCTA 
TGTGCAGCTC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG 
GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG 
GATGTCTTAG AAGGGCTCAA CTTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG 
GTATATTACC TGTCCTGGCT TCGGAACAAG ATCCTACACA ATGACCCAGA GGTGGAAAAG 
AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTACCG TGTTGTGAAA 
TATGAACGGA TTAAGTTCCT GGTCATTGCC CTGAAGAACT CCGTGGAGGT TTATGCCTGG 
GCTCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC 
CCTCTACTGG TGGACCTGAC AGTAGAGGAG GGACAGCGGC TCAAGGTCAT CTATGGCTCC 
AGTGCTGGCT TCCATGCTGT GGATGTTGAT TCTGGGAACA GCTATGACAT CTACATCCCT 
GTACATATCC AGAGCCAGAT CACACCCCAC GCCATCATCT TCCTCCCCAA CACTGATGGC 
ATGGAGATGC TGCTGTGCTA TGAAGATGAG GGTGTCTATG TCAACACTTA CGGGCGGATC 
ATCAAGGATG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTGGCCTA CATCTGCTCC 
AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC 
CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT 
GACAAGGTGT TTTTTGCCTC TGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT 
CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_030246117.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101977.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X8, mRNA.

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CloneID	OMu85833
Clone ID Related Accession (Same CDS sequence)	XM_030246117.1
Accession Version	XM_030246117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTCA AAGAGCGGAC TCTGGATGAG 
GCCCCTAAGC CTCCCAAGAA GGCCATGGAC TACTCCTCAT CCAGTGAGGA GGTGGAAAGC 
AGTGAAGAGG AGGAGGAGGA AGGCGATGGG GAGCCGTCAG AGGGGAGCAG AGACACTCCC 
GGGGGCCGTG ATGGTGATAC AGACAGCGTC AGCACCATGG TGGTTCATGA TGTTGAGGAG 
ATATCCGGGA CCCAGCCCTC ATATGGCGGC GGCACCATGG TGGTCCAGCG TACTCCTGAA 
GAGGAACGAA GCCTGCTGCT TGCTGATAGC AATGGCTACA CAAACCTGCC TGATGTGGTC 
CAGCCCAGCC ACTCACCTAC TGAGAACAGC AAAGGTCAAA GCCCTCCAAC AAAGGATGGA 
GGCAGTGATT ACCAGTCTCG TGGGCTGGTA AAGGCCCCAG GAAAGAGCTC ATTCACCATG 
TTTGTGGATC TAGGGATCTA CCAGCCTGGA GGCAGTGGGG ACACCATCCC TATCACAGCC 
CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT CAACTGCAGT TCGATGTGAG GAAGGGCTCT 
GTGGTCAACG TCAATCCCAC CAACACCCGA GCTCATAGTG AAACTCCTGA AATTCGCAAG 
TACAAGAAGC GATTCAACTC AGAGATCCTA TGTGCAGCTC TCTGGGGGGT CAACCTCCTA 
GTGGGCACAG AGAATGGGCT GATGTTGCTG GACCGAAGTG GGCAGGGCAA GGTGTATGGA 
CTTATTGGGC GACGACGCTT CCAGCAAATG GATGTCTTAG AAGGGCTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTATATTACC TGTCCTGGCT TCGGAACAAG 
ATCCTACACA ATGACCCAGA GGTGGAAAAG AAGCAGGGGT GGACCACCGT GGGGGACATG 
GAGGGCTGCG GCCACTACCG TGTTGTGAAA TATGAACGGA TTAAGTTCCT GGTCATTGCC 
CTGAAGAACT CCGTGGAGGT TTATGCCTGG GCTCCCAAAC CCTACCACAA ATTCATGGCC 
TTCAAGTCCT TTGCTGACCT CCCTCACCGC CCTCTACTGG TGGACCTGAC AGTAGAGGAG 
GGACAGCGGC TCAAGGTCAT CTATGGCTCC AGTGCTGGCT TCCATGCTGT GGATGTTGAT 
TCTGGGAACA GCTATGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACTGATGGC ATGGAGATGC TGCTGTGCTA TGAAGATGAG 
GGTGTCTATG TCAACACTTA CGGGCGGATC ATCAAGGATG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATAA TGGGCTGGGG TGAGAAGGCC 
ATAGAGATCC GCTCTGTGGA GACAGGCCAC CTAGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT GACAAGGTGT TTTTTGCCTC TGTCCGCTCT 
GGAGGAAGCA GCCAAGTTTA CTTTATGACT CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101977.1
CDS	1491..5390
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_030246117.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246117.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTCA AAGAGCGGAC TCTGGATGAG 
GCCCCTAAGC CTCCCAAGAA GGCCATGGAC TACTCCTCAT CCAGTGAGGA GGTGGAAAGC 
AGTGAAGAGG AGGAGGAGGA AGGCGATGGG GAGCCGTCAG AGGGGAGCAG AGACACTCCC 
GGGGGCCGTG ATGGTGATAC AGACAGCGTC AGCACCATGG TGGTTCATGA TGTTGAGGAG 
ATATCCGGGA CCCAGCCCTC ATATGGCGGC GGCACCATGG TGGTCCAGCG TACTCCTGAA 
GAGGAACGAA GCCTGCTGCT TGCTGATAGC AATGGCTACA CAAACCTGCC TGATGTGGTC 
CAGCCCAGCC ACTCACCTAC TGAGAACAGC AAAGGTCAAA GCCCTCCAAC AAAGGATGGA 
GGCAGTGATT ACCAGTCTCG TGGGCTGGTA AAGGCCCCAG GAAAGAGCTC ATTCACCATG 
TTTGTGGATC TAGGGATCTA CCAGCCTGGA GGCAGTGGGG ACACCATCCC TATCACAGCC 
CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT CAACTGCAGT TCGATGTGAG GAAGGGCTCT 
GTGGTCAACG TCAATCCCAC CAACACCCGA GCTCATAGTG AAACTCCTGA AATTCGCAAG 
TACAAGAAGC GATTCAACTC AGAGATCCTA TGTGCAGCTC TCTGGGGGGT CAACCTCCTA 
GTGGGCACAG AGAATGGGCT GATGTTGCTG GACCGAAGTG GGCAGGGCAA GGTGTATGGA 
CTTATTGGGC GACGACGCTT CCAGCAAATG GATGTCTTAG AAGGGCTCAA CTTGCTCATC 
ACCATCTCAG GGAAAAGGAA CAAACTGCGG GTATATTACC TGTCCTGGCT TCGGAACAAG 
ATCCTACACA ATGACCCAGA GGTGGAAAAG AAGCAGGGGT GGACCACCGT GGGGGACATG 
GAGGGCTGCG GCCACTACCG TGTTGTGAAA TATGAACGGA TTAAGTTCCT GGTCATTGCC 
CTGAAGAACT CCGTGGAGGT TTATGCCTGG GCTCCCAAAC CCTACCACAA ATTCATGGCC 
TTCAAGTCCT TTGCTGACCT CCCTCACCGC CCTCTACTGG TGGACCTGAC AGTAGAGGAG 
GGACAGCGGC TCAAGGTCAT CTATGGCTCC AGTGCTGGCT TCCATGCTGT GGATGTTGAT 
TCTGGGAACA GCTATGACAT CTACATCCCT GTACATATCC AGAGCCAGAT CACACCCCAC 
GCCATCATCT TCCTCCCCAA CACTGATGGC ATGGAGATGC TGCTGTGCTA TGAAGATGAG 
GGTGTCTATG TCAACACTTA CGGGCGGATC ATCAAGGATG TGGTGCTGCA GTGGGGAGAG 
ATGCCCACCT CTGTGGCCTA CATCTGCTCC AACCAGATAA TGGGCTGGGG TGAGAAGGCC 
ATAGAGATCC GCTCTGTGGA GACAGGCCAC CTAGATGGGG TCTTCATGCA CAAACGAGCC 
CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT GACAAGGTGT TTTTTGCCTC TGTCCGCTCT 
GGAGGAAGCA GCCAAGTTTA CTTTATGACT CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_030246118.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030101978.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu85834
Clone ID Related Accession (Same CDS sequence)	XM_030246118.1
Accession Version	XM_030246118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCCT TTGCAGAGGA GACAGAGTAT GAGTACAGCG GCAGTGAGGA GGAAGACGAC 
AGCCATGGAG AGGAAGGCGA GCCAAGCTCC ATCATGAATG TGCCCGGTGA GTCCACACTG 
CGCAGAGAAT TCCTCAGACT CCAGCAGGAG AATAAGAGCA ACTCTGAGGC TTTAAAGCAG 
CAGCAGCAGC TGCAGCAACA GCAGCAGCGG GACCCGGAGG CACACATCAA ACACCTGCTG 
CACCAGCGGC AGCGTCGCAT AGAGGAGCAG AAGGAGGAGC GGCGACGTGT GGAGGAGCAA 
CAGCGGCGAG AGCGAGAACA GCGTAAGCTA CAAGAGAAGG AGCAGCAGCG GCGATTGGAA 
GACATGCAAG CCCTACGACG AGAGGAAGAG AGGCGGCAAG CAGAGCGGGA ACAGGAATAT 
ATCCGTCACA GGCTAGAGGA GGAGCAGCGA CAGCTCGAGA TCCTTCAGCA ACAGCTGCTC 
CAGGAACAGG CCTTGCTGCT GGAATACAAG CGGAAGCAGC TGGAGGAGCA GCGGCAGTCA 
GAGCGGCTGC AGAGACAGCT GCAGCAGGAG CACGCCTACC TCAAGTCCCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCTCCA GAAGCAGCAG CAGCAGCAGC AGCAGATCCT GCCTGGAGAC 
AGGAAGCCCC TGTATCATTA CGGTCGGGGC ATTAATCCTG CTGACAAGCC AGCATGGGCC 
CGCGAGGTGG AAGAGAGAGC ACGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCGAAGGCG 
AAGCCAAGCA GTGCGGGGCC AGAGCCCCCC ATCTCCCAGG CCTCTCCTAG CCCCCCAGGA 
CCTCTTTCCC AGACTCCTCC TATGCAGAGG CCTGTGGAGC CCCAGGAAGG ACCGCACAAG 
AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG 
TCCCTGCAGG ACCAGCCGAC TCGAAACCTG GCTGCCTTCC CAGCCTCCCA CGACCCTGAC 
CCTGCTGCTG TCCCTACACC CACTGCCACA CCCAGTGCCC GAGGAGCTGT CATCCGCCAG 
AATTCAGACC CCACCTCTGA AGGGCCAGGG CCTAGCCCAA ACCCTCCATC CTGGGTTCGG 
CCTGATAATG AGGCTCCACC TAAGGTTCCA CAGAGGACCT CTTCTATCGC CACTGCCCTT 
AACACCAGTG GGGCCGGAGG GTCCCGGCCA GCTCAGGCTG TCCGTGCCAG ACCTCGCAGT 
AACTCCGCCT GGCAAATCTA TCTGCAGAGG CGGGCAGAGC GGGGCACCCC CAAGCCTCCC 
GGGCCCCCAG CTCAGCCCCC TGGCCCGCCC AACGCCTCTA GTAACCCTGA CCTCAGGAGG 
AGTGACCCTG GCTGGGAGCG CTCAGACAGT GTCCTCCCGG CCTCCCACGG CCACCTCCCT 
CAGGCTGGCT CCTTGGAGCG GAACCGAAAC CGTGTGGGAG CCTCCACAAA ACTGGATAGC 
TCTCCAGTGC TCTCCCCTGG GAACAAAGCC AAGCCTGAAG ACCACCGCTC AAGGCCAGGC 
CGGCCCGCAA GCTATAAGCG AGCAATTGGC GAGGACTTTG TGTTGCTCAA AGAGCGGACT 
CTGGATGAGG CCCCTAAGCC TCCCAAGAAG GCCATGGACT ACTCCTCATC CAGTGAGGAG 
GTGGAAAGCA GTGAAGAGGA GGAGGAGGAA GGCGATGGGG AGCCGTCAGA GGGGAGCAGA 
GACACTCCCG GGGGCCGCAG TGATGGTGAT ACAGACAGCG TCAGCACCAT GGTGGTTCAT 
GATGTTGAGG AGATATCCGG GACCCAGCCC TCATATGGCG GCGGCACCAT GGTGGTCCAG 
CGTACTCCTG AAGAGGAACG AAGCCTGCTG CTTGCTGATA GCAATGGCTA CACAAACCTG 
CCTGATGTGG TCCAGCCCAG CCACTCACCT ACTGAGAACA GCAAAGGTCA AAGCCCTCCA 
ACAAAGGATG GAGGCAGTGA TTACCAGTCT CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC 
TCATTCACCA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACACCATC 
CCTATCACAG CCCTAGTGGG TGGAGAAGGT GGTCGCCTTG ATCAACTGCA GTTCGATGTG 
AGGAAGGGCT CTGTGGTCAA CGTCAATCCC ACCAACACCC GAGCTCATAG TGAAACTCCT 
GAAATTCGCA AGTACAAGAA GCGATTCAAC TCAGAGATCC TATGTGCAGC TCTCTGGGGG 
GTCAACCTCC TAGTGGGCAC AGAGAATGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC 
AAGGTGTATG GACTTATTGG GCGACGACGC TTCCAGCAAA TGGATGTCTT AGAAGGGCTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTATATTA CCTGTCCTGG 
CTTCGGAACA AGATCCTACA CAATGACCCA GAGGTGGAAA AGAAGCAGGG GTGGACCACC 
GTGGGGGACA TGGAGGGCTG CGGCCACTAC CGTGTTGTGA AATATGAACG GATTAAGTTC 
CTGGTCATTG CCCTGAAGAA CTCCGTGGAG GTTTATGCCT GGGCTCCCAA ACCCTACCAC 
AAATTCATGG CCTTCAAGTC CTTTGCTGAC CTCCCTCACC GCCCTCTACT GGTGGACCTG 
ACAGTAGAGG AGGGACAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT 
GTGGATGTTG ATTCTGGGAA CAGCTATGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACTGATG GCATGGAGAT GCTGCTGTGC 
TATGAAGATG AGGGTGTCTA TGTCAACACT TACGGGCGGA TCATCAAGGA TGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG 
GGTGAGAAGG CCATAGAGAT CCGCTCTGTG GAGACAGGCC ACCTAGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGCA ATGACAAGGT GTTTTTTGCC 
TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT TACTTTATGA CTCTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030101978.1
CDS	933..4124
Translation	MVSFAEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_030246118.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030246118.1

ORF Insert Sequence:

ATGGTCTCCT TTGCAGAGGA GACAGAGTAT GAGTACAGCG GCAGTGAGGA GGAAGACGAC 
AGCCATGGAG AGGAAGGCGA GCCAAGCTCC ATCATGAATG TGCCCGGTGA GTCCACACTG 
CGCAGAGAAT TCCTCAGACT CCAGCAGGAG AATAAGAGCA ACTCTGAGGC TTTAAAGCAG 
CAGCAGCAGC TGCAGCAACA GCAGCAGCGG GACCCGGAGG CACACATCAA ACACCTGCTG 
CACCAGCGGC AGCGTCGCAT AGAGGAGCAG AAGGAGGAGC GGCGACGTGT GGAGGAGCAA 
CAGCGGCGAG AGCGAGAACA GCGTAAGCTA CAAGAGAAGG AGCAGCAGCG GCGATTGGAA 
GACATGCAAG CCCTACGACG AGAGGAAGAG AGGCGGCAAG CAGAGCGGGA ACAGGAATAT 
ATCCGTCACA GGCTAGAGGA GGAGCAGCGA CAGCTCGAGA TCCTTCAGCA ACAGCTGCTC 
CAGGAACAGG CCTTGCTGCT GGAATACAAG CGGAAGCAGC TGGAGGAGCA GCGGCAGTCA 
GAGCGGCTGC AGAGACAGCT GCAGCAGGAG CACGCCTACC TCAAGTCCCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCTCCA GAAGCAGCAG CAGCAGCAGC AGCAGATCCT GCCTGGAGAC 
AGGAAGCCCC TGTATCATTA CGGTCGGGGC ATTAATCCTG CTGACAAGCC AGCATGGGCC 
CGCGAGGTGG AAGAGAGAGC ACGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCGAAGGCG 
AAGCCAAGCA GTGCGGGGCC AGAGCCCCCC ATCTCCCAGG CCTCTCCTAG CCCCCCAGGA 
CCTCTTTCCC AGACTCCTCC TATGCAGAGG CCTGTGGAGC CCCAGGAAGG ACCGCACAAG 
AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG 
TCCCTGCAGG ACCAGCCGAC TCGAAACCTG GCTGCCTTCC CAGCCTCCCA CGACCCTGAC 
CCTGCTGCTG TCCCTACACC CACTGCCACA CCCAGTGCCC GAGGAGCTGT CATCCGCCAG 
AATTCAGACC CCACCTCTGA AGGGCCAGGG CCTAGCCCAA ACCCTCCATC CTGGGTTCGG 
CCTGATAATG AGGCTCCACC TAAGGTTCCA CAGAGGACCT CTTCTATCGC CACTGCCCTT 
AACACCAGTG GGGCCGGAGG GTCCCGGCCA GCTCAGGCTG TCCGTGCCAG ACCTCGCAGT 
AACTCCGCCT GGCAAATCTA TCTGCAGAGG CGGGCAGAGC GGGGCACCCC CAAGCCTCCC 
GGGCCCCCAG CTCAGCCCCC TGGCCCGCCC AACGCCTCTA GTAACCCTGA CCTCAGGAGG 
AGTGACCCTG GCTGGGAGCG CTCAGACAGT GTCCTCCCGG CCTCCCACGG CCACCTCCCT 
CAGGCTGGCT CCTTGGAGCG GAACCGAAAC CGTGTGGGAG CCTCCACAAA ACTGGATAGC 
TCTCCAGTGC TCTCCCCTGG GAACAAAGCC AAGCCTGAAG ACCACCGCTC AAGGCCAGGC 
CGGCCCGCAA GCTATAAGCG AGCAATTGGC GAGGACTTTG TGTTGCTCAA AGAGCGGACT 
CTGGATGAGG CCCCTAAGCC TCCCAAGAAG GCCATGGACT ACTCCTCATC CAGTGAGGAG 
GTGGAAAGCA GTGAAGAGGA GGAGGAGGAA GGCGATGGGG AGCCGTCAGA GGGGAGCAGA 
GACACTCCCG GGGGCCGCAG TGATGGTGAT ACAGACAGCG TCAGCACCAT GGTGGTTCAT 
GATGTTGAGG AGATATCCGG GACCCAGCCC TCATATGGCG GCGGCACCAT GGTGGTCCAG 
CGTACTCCTG AAGAGGAACG AAGCCTGCTG CTTGCTGATA GCAATGGCTA CACAAACCTG 
CCTGATGTGG TCCAGCCCAG CCACTCACCT ACTGAGAACA GCAAAGGTCA AAGCCCTCCA 
ACAAAGGATG GAGGCAGTGA TTACCAGTCT CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC 
TCATTCACCA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACACCATC 
CCTATCACAG CCCTAGTGGG TGGAGAAGGT GGTCGCCTTG ATCAACTGCA GTTCGATGTG 
AGGAAGGGCT CTGTGGTCAA CGTCAATCCC ACCAACACCC GAGCTCATAG TGAAACTCCT 
GAAATTCGCA AGTACAAGAA GCGATTCAAC TCAGAGATCC TATGTGCAGC TCTCTGGGGG 
GTCAACCTCC TAGTGGGCAC AGAGAATGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC 
AAGGTGTATG GACTTATTGG GCGACGACGC TTCCAGCAAA TGGATGTCTT AGAAGGGCTC 
AACTTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTATATTA CCTGTCCTGG 
CTTCGGAACA AGATCCTACA CAATGACCCA GAGGTGGAAA AGAAGCAGGG GTGGACCACC 
GTGGGGGACA TGGAGGGCTG CGGCCACTAC CGTGTTGTGA AATATGAACG GATTAAGTTC 
CTGGTCATTG CCCTGAAGAA CTCCGTGGAG GTTTATGCCT GGGCTCCCAA ACCCTACCAC 
AAATTCATGG CCTTCAAGTC CTTTGCTGAC CTCCCTCACC GCCCTCTACT GGTGGACCTG 
ACAGTAGAGG AGGGACAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT 
GTGGATGTTG ATTCTGGGAA CAGCTATGAC ATCTACATCC CTGTACATAT CCAGAGCCAG 
ATCACACCCC ACGCCATCAT CTTCCTCCCC AACACTGATG GCATGGAGAT GCTGCTGTGC 
TATGAAGATG AGGGTGTCTA TGTCAACACT TACGGGCGGA TCATCAAGGA TGTGGTGCTG 
CAGTGGGGAG AGATGCCCAC CTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG 
GGTGAGAAGG CCATAGAGAT CCGCTCTGTG GAGACAGGCC ACCTAGATGG GGTCTTCATG 
CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGCA ATGACAAGGT GTTTTTTGCC 
TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT TACTTTATGA CTCTGAACCG TAACTGCATC 
ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( NM_176893.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_795712.2 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 2, mRNA.

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CloneID	OMu13990
Clone ID Related Accession (Same CDS sequence)	NM_176893.2
Accession Version	NM_176893.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY206118.1, AL592547.9, BU707794.1 and CJ239135.1. On Sep 6, 2006 this sequence version replaced NM_176893.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB041925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCAA TTGGCGAGGA CTTTGTGTTG 
CTCAAAGAGC GGACTCTGGA TGAGGCCCCT AAGCCTCCCA AGAAGGCCAT GGACTACTCC 
TCATCCAGTG AGGAGGTGGA AAGCAGTGAA GAGGAGGAGG AGGAAGGCGA TGGGGAGCCG 
TCAGAGGGGA GCAGAGACAC TCCCGGGGGC CGCAGTGATG GTGATACAGA CAGCGTCAGC 
ACCATGGTGG TTCATGATGT TGAGGAGATA TCCGGGACCC AGCCCTCATA TGGCGGCGGC 
ACCATGGTGG TCCAGCGTAC TCCTGAAGAG GAACGAAGCC TGCTGCTTGC TGATAGCAAT 
GGCTACACAA ACCTGCCTGA TGTGGTCCAG CCCAGCCACT CACCTACTGA GAACAGCAAA 
GGTCAAAGCC CTCCAACAAA GGATGGAGGC AGTGATTACC AGTCTCGTGG GCTGGTAAAG 
GCCCCAGGAA AGAGCTCATT CACCATGTTT GTGGATCTAG GGATCTACCA GCCTGGAGGC 
AGTGGGGACA CCATCCCTAT CACAGCCCTA GTGGGTGGAG AAGGTGGTCG CCTTGATCAA 
CTGCAGTTCG ATGTGAGGAA GGGCTCTGTG GTCAACGTCA ATCCCACCAA CACCCGAGCT 
CATAGTGAAA CTCCTGAAAT TCGCAAGTAC AAGAAGCGAT TCAACTCAGA GATCCTATGT 
GCAGCTCTCT GGGGGGTCAA CCTCCTAGTG GGCACAGAGA ATGGGCTGAT GTTGCTGGAC 
CGAAGTGGGC AGGGCAAGGT GTATGGACTT ATTGGGCGAC GACGCTTCCA GCAAATGGAT 
GTCTTAGAAG GGCTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTA 
TATTACCTGT CCTGGCTTCG GAACAAGATC CTACACAATG ACCCAGAGGT GGAAAAGAAG 
CAGGGGTGGA CCACCGTGGG GGACATGGAG GGCTGCGGCC ACTACCGTGT TGTGAAATAT 
GAACGGATTA AGTTCCTGGT CATTGCCCTG AAGAACTCCG TGGAGGTTTA TGCCTGGGCT 
CCCAAACCCT ACCACAAATT CATGGCCTTC AAGTCCTTTG CTGACCTCCC TCACCGCCCT 
CTACTGGTGG ACCTGACAGT AGAGGAGGGA CAGCGGCTCA AGGTCATCTA TGGCTCCAGT 
GCTGGCTTCC ATGCTGTGGA TGTTGATTCT GGGAACAGCT ATGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC TGATGGCATG 
GAGATGCTGC TGTGCTATGA AGATGAGGGT GTCTATGTCA ACACTTACGG GCGGATCATC 
AAGGATGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATAATGG GCTGGGGTGA GAAGGCCATA GAGATCCGCT CTGTGGAGAC AGGCCACCTA 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGCAATGAC 
AAGGTGTTTT TTGCCTCTGT CCGCTCTGGA GGAAGCAGCC AAGTTTACTT TATGACTCTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_795712.2
CDS	190..4116
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_176893.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176893.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAAGCTAT AAGCGAGCAA TTGGCGAGGA CTTTGTGTTG 
CTCAAAGAGC GGACTCTGGA TGAGGCCCCT AAGCCTCCCA AGAAGGCCAT GGACTACTCC 
TCATCCAGTG AGGAGGTGGA AAGCAGTGAA GAGGAGGAGG AGGAAGGCGA TGGGGAGCCG 
TCAGAGGGGA GCAGAGACAC TCCCGGGGGC CGCAGTGATG GTGATACAGA CAGCGTCAGC 
ACCATGGTGG TTCATGATGT TGAGGAGATA TCCGGGACCC AGCCCTCATA TGGCGGCGGC 
ACCATGGTGG TCCAGCGTAC TCCTGAAGAG GAACGAAGCC TGCTGCTTGC TGATAGCAAT 
GGCTACACAA ACCTGCCTGA TGTGGTCCAG CCCAGCCACT CACCTACTGA GAACAGCAAA 
GGTCAAAGCC CTCCAACAAA GGATGGAGGC AGTGATTACC AGTCTCGTGG GCTGGTAAAG 
GCCCCAGGAA AGAGCTCATT CACCATGTTT GTGGATCTAG GGATCTACCA GCCTGGAGGC 
AGTGGGGACA CCATCCCTAT CACAGCCCTA GTGGGTGGAG AAGGTGGTCG CCTTGATCAA 
CTGCAGTTCG ATGTGAGGAA GGGCTCTGTG GTCAACGTCA ATCCCACCAA CACCCGAGCT 
CATAGTGAAA CTCCTGAAAT TCGCAAGTAC AAGAAGCGAT TCAACTCAGA GATCCTATGT 
GCAGCTCTCT GGGGGGTCAA CCTCCTAGTG GGCACAGAGA ATGGGCTGAT GTTGCTGGAC 
CGAAGTGGGC AGGGCAAGGT GTATGGACTT ATTGGGCGAC GACGCTTCCA GCAAATGGAT 
GTCTTAGAAG GGCTCAACTT GCTCATCACC ATCTCAGGGA AAAGGAACAA ACTGCGGGTA 
TATTACCTGT CCTGGCTTCG GAACAAGATC CTACACAATG ACCCAGAGGT GGAAAAGAAG 
CAGGGGTGGA CCACCGTGGG GGACATGGAG GGCTGCGGCC ACTACCGTGT TGTGAAATAT 
GAACGGATTA AGTTCCTGGT CATTGCCCTG AAGAACTCCG TGGAGGTTTA TGCCTGGGCT 
CCCAAACCCT ACCACAAATT CATGGCCTTC AAGTCCTTTG CTGACCTCCC TCACCGCCCT 
CTACTGGTGG ACCTGACAGT AGAGGAGGGA CAGCGGCTCA AGGTCATCTA TGGCTCCAGT 
GCTGGCTTCC ATGCTGTGGA TGTTGATTCT GGGAACAGCT ATGACATCTA CATCCCTGTA 
CATATCCAGA GCCAGATCAC ACCCCACGCC ATCATCTTCC TCCCCAACAC TGATGGCATG 
GAGATGCTGC TGTGCTATGA AGATGAGGGT GTCTATGTCA ACACTTACGG GCGGATCATC 
AAGGATGTGG TGCTGCAGTG GGGAGAGATG CCCACCTCTG TGGCCTACAT CTGCTCCAAC 
CAGATAATGG GCTGGGGTGA GAAGGCCATA GAGATCCGCT CTGTGGAGAC AGGCCACCTA 
GATGGGGTCT TCATGCACAA ACGAGCCCAG AGGCTCAAGT TCCTGTGTGA GCGCAATGAC 
AAGGTGTTTT TTGCCTCTGT CCGCTCTGGA GGAAGCAGCC AAGTTTACTT TATGACTCTG 
AACCGTAACT GCATCATGAA CTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( NM_001045964.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001039429.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 4, mRNA.

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CloneID	OMu14018
Clone ID Related Accession (Same CDS sequence)	NM_001045964.1
Accession Version	NM_001045964.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592547.9, CB245214.1, CD350084.1, CF736764.1 and CJ239135.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGTGATGGT GATACAGACA GCGTCAGCAC CATGGTGGTT 
CATGATGTTG AGGAGATATC CGGGACCCAG CCCTCATATG GCGGCGGCAC CATGGTGGTC 
CAGCGTACTC CTGAAGAGGA ACGAAGCCTG CTGCTTGCTG ATAGCAATGG CTACACAAAC 
CTGCCTGATG TGGTCCAGCC CAGCCACTCA CCTACTGAGA ACAGCAAAGG TCAAAGCCCT 
CCAACAAAGG ATGGAGGCAG TGATTACCAG TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG 
AGCTCATTCA CCATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGACACC 
ATCCCTATCA CAGCCCTAGT GGGTGGAGAA GGTGGTCGCC TTGATCAACT GCAGTTCGAT 
GTGAGGAAGG GCTCTGTGGT CAACGTCAAT CCCACCAACA CCCGAGCTCA TAGTGAAACT 
CCTGAAATTC GCAAGTACAA GAAGCGATTC AACTCAGAGA TCCTATGTGC AGCTCTCTGG 
GGGGTCAACC TCCTAGTGGG CACAGAGAAT GGGCTGATGT TGCTGGACCG AAGTGGGCAG 
GGCAAGGTGT ATGGACTTAT TGGGCGACGA CGCTTCCAGC AAATGGATGT CTTAGAAGGG 
CTCAACTTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTATA TTACCTGTCC 
TGGCTTCGGA ACAAGATCCT ACACAATGAC CCAGAGGTGG AAAAGAAGCA GGGGTGGACC 
ACCGTGGGGG ACATGGAGGG CTGCGGCCAC TACCGTGTTG TGAAATATGA ACGGATTAAG 
TTCCTGGTCA TTGCCCTGAA GAACTCCGTG GAGGTTTATG CCTGGGCTCC CAAACCCTAC 
CACAAATTCA TGGCCTTCAA GTCCTTTGCT GACCTCCCTC ACCGCCCTCT ACTGGTGGAC 
CTGACAGTAG AGGAGGGACA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT 
GCTGTGGATG TTGATTCTGG GAACAGCTAT GACATCTACA TCCCTGTACA TATCCAGAGC 
CAGATCACAC CCCACGCCAT CATCTTCCTC CCCAACACTG ATGGCATGGA GATGCTGCTG 
TGCTATGAAG ATGAGGGTGT CTATGTCAAC ACTTACGGGC GGATCATCAA GGATGTGGTG 
CTGCAGTGGG GAGAGATGCC CACCTCTGTG GCCTACATCT GCTCCAACCA GATAATGGGC 
TGGGGTGAGA AGGCCATAGA GATCCGCTCT GTGGAGACAG GCCACCTAGA TGGGGTCTTC 
ATGCACAAAC GAGCCCAGAG GCTCAAGTTC CTGTGTGAGC GCAATGACAA GGTGTTTTTT 
GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA GTTTACTTTA TGACTCTGAA CCGTAACTGC 
ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039429.1
CDS	190..4224
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_001045964.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045964.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGTGATGGT GATACAGACA GCGTCAGCAC CATGGTGGTT 
CATGATGTTG AGGAGATATC CGGGACCCAG CCCTCATATG GCGGCGGCAC CATGGTGGTC 
CAGCGTACTC CTGAAGAGGA ACGAAGCCTG CTGCTTGCTG ATAGCAATGG CTACACAAAC 
CTGCCTGATG TGGTCCAGCC CAGCCACTCA CCTACTGAGA ACAGCAAAGG TCAAAGCCCT 
CCAACAAAGG ATGGAGGCAG TGATTACCAG TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG 
AGCTCATTCA CCATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGACACC 
ATCCCTATCA CAGCCCTAGT GGGTGGAGAA GGTGGTCGCC TTGATCAACT GCAGTTCGAT 
GTGAGGAAGG GCTCTGTGGT CAACGTCAAT CCCACCAACA CCCGAGCTCA TAGTGAAACT 
CCTGAAATTC GCAAGTACAA GAAGCGATTC AACTCAGAGA TCCTATGTGC AGCTCTCTGG 
GGGGTCAACC TCCTAGTGGG CACAGAGAAT GGGCTGATGT TGCTGGACCG AAGTGGGCAG 
GGCAAGGTGT ATGGACTTAT TGGGCGACGA CGCTTCCAGC AAATGGATGT CTTAGAAGGG 
CTCAACTTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTATA TTACCTGTCC 
TGGCTTCGGA ACAAGATCCT ACACAATGAC CCAGAGGTGG AAAAGAAGCA GGGGTGGACC 
ACCGTGGGGG ACATGGAGGG CTGCGGCCAC TACCGTGTTG TGAAATATGA ACGGATTAAG 
TTCCTGGTCA TTGCCCTGAA GAACTCCGTG GAGGTTTATG CCTGGGCTCC CAAACCCTAC 
CACAAATTCA TGGCCTTCAA GTCCTTTGCT GACCTCCCTC ACCGCCCTCT ACTGGTGGAC 
CTGACAGTAG AGGAGGGACA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT 
GCTGTGGATG TTGATTCTGG GAACAGCTAT GACATCTACA TCCCTGTACA TATCCAGAGC 
CAGATCACAC CCCACGCCAT CATCTTCCTC CCCAACACTG ATGGCATGGA GATGCTGCTG 
TGCTATGAAG ATGAGGGTGT CTATGTCAAC ACTTACGGGC GGATCATCAA GGATGTGGTG 
CTGCAGTGGG GAGAGATGCC CACCTCTGTG GCCTACATCT GCTCCAACCA GATAATGGGC 
TGGGGTGAGA AGGCCATAGA GATCCGCTCT GTGGAGACAG GCCACCTAGA TGGGGTCTTC 
ATGCACAAAC GAGCCCAGAG GCTCAAGTTC CTGTGTGAGC GCAATGACAA GGTGTTTTTT 
GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA GTTTACTTTA TGACTCTGAA CCGTAACTGC 
ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( NM_016713.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_057922.2 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14043
Clone ID Related Accession (Same CDS sequence)	NM_016713.2
Accession Version	NM_016713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY206118.1, AL592547.9 and CJ239135.1. On or before Sep 13, 2007 this sequence version replaced XM_001471583.1, XM_001471584.1, XM_001471687.1, XM_001471869.1, NM_016713.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTCA AAGAGCGGAC TCTGGATGAG 
GCCCCTAAGC CTCCCAAGAA GGCCATGGAC TACTCCTCAT CCAGTGAGGA GGTGGAAAGC 
AGTGAAGAGG AGGAGGAGGA AGGCGATGGG GAGCCGTCAG AGGGGAGCAG AGACACTCCC 
GGGGGCCGCA GTGATGGTGA TACAGACAGC GTCAGCACCA TGGTGGTTCA TGATGTTGAG 
GAGATATCCG GGACCCAGCC CTCATATGGC GGCGGCACCA TGGTGGTCCA GCGTACTCCT 
GAAGAGGAAC GAAGCCTGCT GCTTGCTGAT AGCAATGGCT ACACAAACCT GCCTGATGTG 
GTCCAGCCCA GCCACTCACC TACTGAGAAC AGCAAAGGTC AAAGCCCTCC AACAAAGGAT 
GGAGGCAGTG ATTACCAGTC TCGTGGGCTG GTAAAGGCCC CAGGAAAGAG CTCATTCACC 
ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACACCAT CCCTATCACA 
GCCCTAGTGG GTGGAGAAGG TGGTCGCCTT GATCAACTGC AGTTCGATGT GAGGAAGGGC 
TCTGTGGTCA ACGTCAATCC CACCAACACC CGAGCTCATA GTGAAACTCC TGAAATTCGC 
AAGTACAAGA AGCGATTCAA CTCAGAGATC CTATGTGCAG CTCTCTGGGG GGTCAACCTC 
CTAGTGGGCA CAGAGAATGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT 
GGACTTATTG GGCGACGACG CTTCCAGCAA ATGGATGTCT TAGAAGGGCT CAACTTGCTC 
ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTATATT ACCTGTCCTG GCTTCGGAAC 
AAGATCCTAC ACAATGACCC AGAGGTGGAA AAGAAGCAGG GGTGGACCAC CGTGGGGGAC 
ATGGAGGGCT GCGGCCACTA CCGTGTTGTG AAATATGAAC GGATTAAGTT CCTGGTCATT 
GCCCTGAAGA ACTCCGTGGA GGTTTATGCC TGGGCTCCCA AACCCTACCA CAAATTCATG 
GCCTTCAAGT CCTTTGCTGA CCTCCCTCAC CGCCCTCTAC TGGTGGACCT GACAGTAGAG 
GAGGGACAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTT 
GATTCTGGGA ACAGCTATGA CATCTACATC CCTGTACATA TCCAGAGCCA GATCACACCC 
CACGCCATCA TCTTCCTCCC CAACACTGAT GGCATGGAGA TGCTGCTGTG CTATGAAGAT 
GAGGGTGTCT ATGTCAACAC TTACGGGCGG ATCATCAAGG ATGTGGTGCT GCAGTGGGGA 
GAGATGCCCA CCTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAG 
GCCATAGAGA TCCGCTCTGT GGAGACAGGC CACCTAGATG GGGTCTTCAT GCACAAACGA 
GCCCAGAGGC TCAAGTTCCT GTGTGAGCGC AATGACAAGG TGTTTTTTGC CTCTGTCCGC 
TCTGGAGGAA GCAGCCAAGT TTACTTTATG ACTCTGAACC GTAACTGCAT CATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057922.2
CDS	190..4092
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_016713.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016713.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC 
CCTGACCTCA GGAGGAGTGA CCCTGGCTGG GAGCGCTCAG ACAGTGTCCT CCCGGCCTCC 
CACGGCCACC TCCCTCAGGC TGGCTCCTTG GAGCGGAACC GAAACCGTGT GGGAGCCTCC 
ACAAAACTGG ATAGCTCTCC AGTGCTCTCC CCTGGGAACA AAGCCAAGCC TGAAGACCAC 
CGCTCAAGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTCA AAGAGCGGAC TCTGGATGAG 
GCCCCTAAGC CTCCCAAGAA GGCCATGGAC TACTCCTCAT CCAGTGAGGA GGTGGAAAGC 
AGTGAAGAGG AGGAGGAGGA AGGCGATGGG GAGCCGTCAG AGGGGAGCAG AGACACTCCC 
GGGGGCCGCA GTGATGGTGA TACAGACAGC GTCAGCACCA TGGTGGTTCA TGATGTTGAG 
GAGATATCCG GGACCCAGCC CTCATATGGC GGCGGCACCA TGGTGGTCCA GCGTACTCCT 
GAAGAGGAAC GAAGCCTGCT GCTTGCTGAT AGCAATGGCT ACACAAACCT GCCTGATGTG 
GTCCAGCCCA GCCACTCACC TACTGAGAAC AGCAAAGGTC AAAGCCCTCC AACAAAGGAT 
GGAGGCAGTG ATTACCAGTC TCGTGGGCTG GTAAAGGCCC CAGGAAAGAG CTCATTCACC 
ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACACCAT CCCTATCACA 
GCCCTAGTGG GTGGAGAAGG TGGTCGCCTT GATCAACTGC AGTTCGATGT GAGGAAGGGC 
TCTGTGGTCA ACGTCAATCC CACCAACACC CGAGCTCATA GTGAAACTCC TGAAATTCGC 
AAGTACAAGA AGCGATTCAA CTCAGAGATC CTATGTGCAG CTCTCTGGGG GGTCAACCTC 
CTAGTGGGCA CAGAGAATGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT 
GGACTTATTG GGCGACGACG CTTCCAGCAA ATGGATGTCT TAGAAGGGCT CAACTTGCTC 
ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTATATT ACCTGTCCTG GCTTCGGAAC 
AAGATCCTAC ACAATGACCC AGAGGTGGAA AAGAAGCAGG GGTGGACCAC CGTGGGGGAC 
ATGGAGGGCT GCGGCCACTA CCGTGTTGTG AAATATGAAC GGATTAAGTT CCTGGTCATT 
GCCCTGAAGA ACTCCGTGGA GGTTTATGCC TGGGCTCCCA AACCCTACCA CAAATTCATG 
GCCTTCAAGT CCTTTGCTGA CCTCCCTCAC CGCCCTCTAC TGGTGGACCT GACAGTAGAG 
GAGGGACAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTT 
GATTCTGGGA ACAGCTATGA CATCTACATC CCTGTACATA TCCAGAGCCA GATCACACCC 
CACGCCATCA TCTTCCTCCC CAACACTGAT GGCATGGAGA TGCTGCTGTG CTATGAAGAT 
GAGGGTGTCT ATGTCAACAC TTACGGGCGG ATCATCAAGG ATGTGGTGCT GCAGTGGGGA 
GAGATGCCCA CCTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAG 
GCCATAGAGA TCCGCTCTGT GGAGACAGGC CACCTAGATG GGGTCTTCAT GCACAAACGA 
GCCCAGAGGC TCAAGTTCCT GTGTGAGCGC AATGACAAGG TGTTTTTTGC CTCTGTCCGC 
TCTGGAGGAA GCAGCCAAGT TTACTTTATG ACTCTGAACC GTAACTGCAT CATGAACTGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( NM_001045959.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001039424.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 3, mRNA.

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CloneID	OMu14135
Clone ID Related Accession (Same CDS sequence)	NM_001045959.1
Accession Version	NM_001045959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592547.9, BC052474.1, CD350084.1, BM932728.1 and CJ239135.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001039424.1
CDS	190..4203
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	NM_001045959.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045959.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533740.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.

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CloneID	OMu46565
Clone ID Related Accession (Same CDS sequence)	XM_006533677.2 , XM_006533677.3
Accession Version	XM_006533677.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG CAGTGATGGT 
GATACAGACA GCGTCAGCAC CATGGTGGTT CATGATGTTG AGGAGATATC CGGGACCCAG 
CCCTCATATG GCGGCGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA ACGAAGCCTG 
CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCTGATG TGGTCCAGCC CAGCCACTCA 
CCTACTGAGA ACAGCAAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCAG TGATTACCAG 
TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGCTCATTCA CCATGTTTGT GGATCTAGGG 
ATCTACCAGC CTGGAGGCAG TGGGGACACC ATCCCTATCA CAGCCCTAGT GGGTGGAGAA 
GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT 
CCCACCAACA CCCGAGCTCA TAGTGAAACT CCTGAAATTC GCAAGTACAA GAAGCGATTC 
AACTCAGAGA TCCTATGTGC AGCTCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT 
GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAGGTGT ATGGACTTAT TGGGCGACGA 
CGCTTCCAGC AAATGGATGT CTTAGAAGGG CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ACAAGATCCT ACACAATGAC 
CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC 
TACCGTGTTG TGAAATATGA ACGGATTAAG TTCCTGGTCA TTGCCCTGAA GAACTCCGTG 
GAGGTTTATG CCTGGGCTCC CAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT 
GACCTCCCTC ACCGCCCTCT ACTGGTGGAC CTGACAGTAG AGGAGGGACA GCGGCTCAAG 
GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGATTCTGG GAACAGCTAT 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACTG ATGGCATGGA GATGCTGCTG TGCTATGAAG ATGAGGGTGT CTATGTCAAC 
ACTTACGGGC GGATCATCAA GGATGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT 
GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GCAATGACAA GGTGTTTTTT GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA 
GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533740.1
CDS	399..4523
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533677.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG CAGTGATGGT 
GATACAGACA GCGTCAGCAC CATGGTGGTT CATGATGTTG AGGAGATATC CGGGACCCAG 
CCCTCATATG GCGGCGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA ACGAAGCCTG 
CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCTGATG TGGTCCAGCC CAGCCACTCA 
CCTACTGAGA ACAGCAAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCAG TGATTACCAG 
TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGCTCATTCA CCATGTTTGT GGATCTAGGG 
ATCTACCAGC CTGGAGGCAG TGGGGACACC ATCCCTATCA CAGCCCTAGT GGGTGGAGAA 
GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT 
CCCACCAACA CCCGAGCTCA TAGTGAAACT CCTGAAATTC GCAAGTACAA GAAGCGATTC 
AACTCAGAGA TCCTATGTGC AGCTCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT 
GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAGGTGT ATGGACTTAT TGGGCGACGA 
CGCTTCCAGC AAATGGATGT CTTAGAAGGG CTCAACTTGC TCATCACCAT CTCAGGGAAA 
AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ACAAGATCCT ACACAATGAC 
CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC 
TACCGTGTTG TGAAATATGA ACGGATTAAG TTCCTGGTCA TTGCCCTGAA GAACTCCGTG 
GAGGTTTATG CCTGGGCTCC CAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT 
GACCTCCCTC ACCGCCCTCT ACTGGTGGAC CTGACAGTAG AGGAGGGACA GCGGCTCAAG 
GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGATTCTGG GAACAGCTAT 
GACATCTACA TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC 
CCCAACACTG ATGGCATGGA GATGCTGCTG TGCTATGAAG ATGAGGGTGT CTATGTCAAC 
ACTTACGGGC GGATCATCAA GGATGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG 
GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT 
GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC 
CTGTGTGAGC GCAATGACAA GGTGTTTTTT GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA 
GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533678.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533741.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46566
Clone ID Related Accession (Same CDS sequence)	XM_006533678.2 , XM_006533678.3
Accession Version	XM_006533678.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG TGATGGTGAT 
ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTTGAGG AGATATCCGG GACCCAGCCC 
TCATATGGCG GCGGCACCAT GGTGGTCCAG CGTACTCCTG AAGAGGAACG AAGCCTGCTG 
CTTGCTGATA GCAATGGCTA CACAAACCTG CCTGATGTGG TCCAGCCCAG CCACTCACCT 
ACTGAGAACA GCAAAGGTCA AAGCCCTCCA ACAAAGGATG GAGGCAGTGA TTACCAGTCT 
CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC TCATTCACCA TGTTTGTGGA TCTAGGGATC 
TACCAGCCTG GAGGCAGTGG GGACACCATC CCTATCACAG CCCTAGTGGG TGGAGAAGGT 
GGTCGCCTTG ATCAACTGCA GTTCGATGTG AGGAAGGGCT CTGTGGTCAA CGTCAATCCC 
ACCAACACCC GAGCTCATAG TGAAACTCCT GAAATTCGCA AGTACAAGAA GCGATTCAAC 
TCAGAGATCC TATGTGCAGC TCTCTGGGGG GTCAACCTCC TAGTGGGCAC AGAGAATGGG 
CTGATGTTGC TGGACCGAAG TGGGCAGGGC AAGGTGTATG GACTTATTGG GCGACGACGC 
TTCCAGCAAA TGGATGTCTT AGAAGGGCTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTATATTA CCTGTCCTGG CTTCGGAACA AGATCCTACA CAATGACCCA 
GAGGTGGAAA AGAAGCAGGG GTGGACCACC GTGGGGGACA TGGAGGGCTG CGGCCACTAC 
CGTGTTGTGA AATATGAACG GATTAAGTTC CTGGTCATTG CCCTGAAGAA CTCCGTGGAG 
GTTTATGCCT GGGCTCCCAA ACCCTACCAC AAATTCATGG CCTTCAAGTC CTTTGCTGAC 
CTCCCTCACC GCCCTCTACT GGTGGACCTG ACAGTAGAGG AGGGACAGCG GCTCAAGGTC 
ATCTATGGCT CCAGTGCTGG CTTCCATGCT GTGGATGTTG ATTCTGGGAA CAGCTATGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACTGATG GCATGGAGAT GCTGCTGTGC TATGAAGATG AGGGTGTCTA TGTCAACACT 
TACGGGCGGA TCATCAAGGA TGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT AATGGGCTGG GGTGAGAAGG CCATAGAGAT CCGCTCTGTG 
GAGACAGGCC ACCTAGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGCA ATGACAAGGT GTTTTTTGCC TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT 
TACTTTATGA CTCTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533741.1
CDS	403..4524
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533678.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533678.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT 
GGCGAGGACT TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG 
AAGGCCATGG ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG 
GAAGGCGATG GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG TGATGGTGAT 
ACAGACAGCG TCAGCACCAT GGTGGTTCAT GATGTTGAGG AGATATCCGG GACCCAGCCC 
TCATATGGCG GCGGCACCAT GGTGGTCCAG CGTACTCCTG AAGAGGAACG AAGCCTGCTG 
CTTGCTGATA GCAATGGCTA CACAAACCTG CCTGATGTGG TCCAGCCCAG CCACTCACCT 
ACTGAGAACA GCAAAGGTCA AAGCCCTCCA ACAAAGGATG GAGGCAGTGA TTACCAGTCT 
CGTGGGCTGG TAAAGGCCCC AGGAAAGAGC TCATTCACCA TGTTTGTGGA TCTAGGGATC 
TACCAGCCTG GAGGCAGTGG GGACACCATC CCTATCACAG CCCTAGTGGG TGGAGAAGGT 
GGTCGCCTTG ATCAACTGCA GTTCGATGTG AGGAAGGGCT CTGTGGTCAA CGTCAATCCC 
ACCAACACCC GAGCTCATAG TGAAACTCCT GAAATTCGCA AGTACAAGAA GCGATTCAAC 
TCAGAGATCC TATGTGCAGC TCTCTGGGGG GTCAACCTCC TAGTGGGCAC AGAGAATGGG 
CTGATGTTGC TGGACCGAAG TGGGCAGGGC AAGGTGTATG GACTTATTGG GCGACGACGC 
TTCCAGCAAA TGGATGTCTT AGAAGGGCTC AACTTGCTCA TCACCATCTC AGGGAAAAGG 
AACAAACTGC GGGTATATTA CCTGTCCTGG CTTCGGAACA AGATCCTACA CAATGACCCA 
GAGGTGGAAA AGAAGCAGGG GTGGACCACC GTGGGGGACA TGGAGGGCTG CGGCCACTAC 
CGTGTTGTGA AATATGAACG GATTAAGTTC CTGGTCATTG CCCTGAAGAA CTCCGTGGAG 
GTTTATGCCT GGGCTCCCAA ACCCTACCAC AAATTCATGG CCTTCAAGTC CTTTGCTGAC 
CTCCCTCACC GCCCTCTACT GGTGGACCTG ACAGTAGAGG AGGGACAGCG GCTCAAGGTC 
ATCTATGGCT CCAGTGCTGG CTTCCATGCT GTGGATGTTG ATTCTGGGAA CAGCTATGAC 
ATCTACATCC CTGTACATAT CCAGAGCCAG ATCACACCCC ACGCCATCAT CTTCCTCCCC 
AACACTGATG GCATGGAGAT GCTGCTGTGC TATGAAGATG AGGGTGTCTA TGTCAACACT 
TACGGGCGGA TCATCAAGGA TGTGGTGCTG CAGTGGGGAG AGATGCCCAC CTCTGTGGCC 
TACATCTGCT CCAACCAGAT AATGGGCTGG GGTGAGAAGG CCATAGAGAT CCGCTCTGTG 
GAGACAGGCC ACCTAGATGG GGTCTTCATG CACAAACGAG CCCAGAGGCT CAAGTTCCTG 
TGTGAGCGCA ATGACAAGGT GTTTTTTGCC TCTGTCCGCT CTGGAGGAAG CAGCCAAGTT 
TACTTTATGA CTCTGAACCG TAACTGCATC ATGAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533679.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533742.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.

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CloneID	OMu46567
Clone ID Related Accession (Same CDS sequence)	XM_006533679.3 , XM_006533679.2
Accession Version	XM_006533679.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTCAAA 
GAGCGGACTC TGGATGAGGC CCCTAAGCCT CCCAAGAAGG CCATGGACTA CTCCTCATCC 
AGTGAGGAGG TGGAAAGCAG TGAAGAGGAG GAGGAGGAAG GCGATGGGGA GCCGTCAGAG 
GGGAGCAGAG ACACTCCCGG GGGCCGCAGT GATGGTGATA CAGACAGCGT CAGCACCATG 
GTGGTTCATG ATGTTGAGGA GATATCCGGG ACCCAGCCCT CATATGGCGG CGGCACCATG 
GTGGTCCAGC GTACTCCTGA AGAGGAACGA AGCCTGCTGC TTGCTGATAG CAATGGCTAC 
ACAAACCTGC CTGATGTGGT CCAGCCCAGC CACTCACCTA CTGAGAACAG CAAAGGTCAA 
AGCCCTCCAA CAAAGGATGG AGGCAGTGAT TACCAGTCTC GTGGGCTGGT AAAGGCCCCA 
GGAAAGAGCT CATTCACCAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG 
GACACCATCC CTATCACAGC CCTAGTGGGT GGAGAAGGTG GTCGCCTTGA TCAACTGCAG 
TTCGATGTGA GGAAGGGCTC TGTGGTCAAC GTCAATCCCA CCAACACCCG AGCTCATAGT 
GAAACTCCTG AAATTCGCAA GTACAAGAAG CGATTCAACT CAGAGATCCT ATGTGCAGCT 
CTCTGGGGGG TCAACCTCCT AGTGGGCACA GAGAATGGGC TGATGTTGCT GGACCGAAGT 
GGGCAGGGCA AGGTGTATGG ACTTATTGGG CGACGACGCT TCCAGCAAAT GGATGTCTTA 
GAAGGGCTCA ACTTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTATATTAC 
CTGTCCTGGC TTCGGAACAA GATCCTACAC AATGACCCAG AGGTGGAAAA GAAGCAGGGG 
TGGACCACCG TGGGGGACAT GGAGGGCTGC GGCCACTACC GTGTTGTGAA ATATGAACGG 
ATTAAGTTCC TGGTCATTGC CCTGAAGAAC TCCGTGGAGG TTTATGCCTG GGCTCCCAAA 
CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCTGACC TCCCTCACCG CCCTCTACTG 
GTGGACCTGA CAGTAGAGGA GGGACAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC 
TTCCATGCTG TGGATGTTGA TTCTGGGAAC AGCTATGACA TCTACATCCC TGTACATATC 
CAGAGCCAGA TCACACCCCA CGCCATCATC TTCCTCCCCA ACACTGATGG CATGGAGATG 
CTGCTGTGCT ATGAAGATGA GGGTGTCTAT GTCAACACTT ACGGGCGGAT CATCAAGGAT 
GTGGTGCTGC AGTGGGGAGA GATGCCCACC TCTGTGGCCT ACATCTGCTC CAACCAGATA 
ATGGGCTGGG GTGAGAAGGC CATAGAGATC CGCTCTGTGG AGACAGGCCA CCTAGATGGG 
GTCTTCATGC ACAAACGAGC CCAGAGGCTC AAGTTCCTGT GTGAGCGCAA TGACAAGGTG 
TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC AGCCAAGTTT ACTTTATGAC TCTGAACCGT 
AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533742.1
CDS	398..4498
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533679.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533679.2

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGACCTCGC AGTAACTCCG CCTGGCAAAT CTATCTGCAG 
AGGCGGGCAG AGCGGGGCAC CCCCAAGCCT CCCGGGCCCC CAGCTCAGCC CCCTGGCCCG 
CCCAACGCCT CTAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC 
AGTGTCCTCC CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA 
AACCGTGTGG GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA 
GCCAAGCCTG AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTCAAA 
GAGCGGACTC TGGATGAGGC CCCTAAGCCT CCCAAGAAGG CCATGGACTA CTCCTCATCC 
AGTGAGGAGG TGGAAAGCAG TGAAGAGGAG GAGGAGGAAG GCGATGGGGA GCCGTCAGAG 
GGGAGCAGAG ACACTCCCGG GGGCCGCAGT GATGGTGATA CAGACAGCGT CAGCACCATG 
GTGGTTCATG ATGTTGAGGA GATATCCGGG ACCCAGCCCT CATATGGCGG CGGCACCATG 
GTGGTCCAGC GTACTCCTGA AGAGGAACGA AGCCTGCTGC TTGCTGATAG CAATGGCTAC 
ACAAACCTGC CTGATGTGGT CCAGCCCAGC CACTCACCTA CTGAGAACAG CAAAGGTCAA 
AGCCCTCCAA CAAAGGATGG AGGCAGTGAT TACCAGTCTC GTGGGCTGGT AAAGGCCCCA 
GGAAAGAGCT CATTCACCAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG 
GACACCATCC CTATCACAGC CCTAGTGGGT GGAGAAGGTG GTCGCCTTGA TCAACTGCAG 
TTCGATGTGA GGAAGGGCTC TGTGGTCAAC GTCAATCCCA CCAACACCCG AGCTCATAGT 
GAAACTCCTG AAATTCGCAA GTACAAGAAG CGATTCAACT CAGAGATCCT ATGTGCAGCT 
CTCTGGGGGG TCAACCTCCT AGTGGGCACA GAGAATGGGC TGATGTTGCT GGACCGAAGT 
GGGCAGGGCA AGGTGTATGG ACTTATTGGG CGACGACGCT TCCAGCAAAT GGATGTCTTA 
GAAGGGCTCA ACTTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTATATTAC 
CTGTCCTGGC TTCGGAACAA GATCCTACAC AATGACCCAG AGGTGGAAAA GAAGCAGGGG 
TGGACCACCG TGGGGGACAT GGAGGGCTGC GGCCACTACC GTGTTGTGAA ATATGAACGG 
ATTAAGTTCC TGGTCATTGC CCTGAAGAAC TCCGTGGAGG TTTATGCCTG GGCTCCCAAA 
CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCTGACC TCCCTCACCG CCCTCTACTG 
GTGGACCTGA CAGTAGAGGA GGGACAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC 
TTCCATGCTG TGGATGTTGA TTCTGGGAAC AGCTATGACA TCTACATCCC TGTACATATC 
CAGAGCCAGA TCACACCCCA CGCCATCATC TTCCTCCCCA ACACTGATGG CATGGAGATG 
CTGCTGTGCT ATGAAGATGA GGGTGTCTAT GTCAACACTT ACGGGCGGAT CATCAAGGAT 
GTGGTGCTGC AGTGGGGAGA GATGCCCACC TCTGTGGCCT ACATCTGCTC CAACCAGATA 
ATGGGCTGGG GTGAGAAGGC CATAGAGATC CGCTCTGTGG AGACAGGCCA CCTAGATGGG 
GTCTTCATGC ACAAACGAGC CCAGAGGCTC AAGTTCCTGT GTGAGCGCAA TGACAAGGTG 
TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC AGCCAAGTTT ACTTTATGAC TCTGAACCGT 
AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533681.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533744.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu46569
Clone ID Related Accession (Same CDS sequence)	XM_006533681.3 , XM_006533681.4
Accession Version	XM_006533681.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533681.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGCAGTGAT GGTGATACAG ACAGCGTCAG CACCATGGTG 
GTTCATGATG TTGAGGAGAT ATCCGGGACC CAGCCCTCAT ATGGCGGCGG CACCATGGTG 
GTCCAGCGTA CTCCTGAAGA GGAACGAAGC CTGCTGCTTG CTGATAGCAA TGGCTACACA 
AACCTGCCTG ATGTGGTCCA GCCCAGCCAC TCACCTACTG AGAACAGCAA AGGTCAAAGC 
CCTCCAACAA AGGATGGAGG CAGTGATTAC CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA 
AAGAGCTCAT TCACCATGTT TGTGGATCTA GGGATCTACC AGCCTGGAGG CAGTGGGGAC 
ACCATCCCTA TCACAGCCCT AGTGGGTGGA GAAGGTGGTC GCCTTGATCA ACTGCAGTTC 
GATGTGAGGA AGGGCTCTGT GGTCAACGTC AATCCCACCA ACACCCGAGC TCATAGTGAA 
ACTCCTGAAA TTCGCAAGTA CAAGAAGCGA TTCAACTCAG AGATCCTATG TGCAGCTCTC 
TGGGGGGTCA ACCTCCTAGT GGGCACAGAG AATGGGCTGA TGTTGCTGGA CCGAAGTGGG 
CAGGGCAAGG TGTATGGACT TATTGGGCGA CGACGCTTCC AGCAAATGGA TGTCTTAGAA 
GGGCTCAACT TGCTCATCAC CATCTCAGGG AAAAGGAACA AACTGCGGGT ATATTACCTG 
TCCTGGCTTC GGAACAAGAT CCTACACAAT GACCCAGAGG TGGAAAAGAA GCAGGGGTGG 
ACCACCGTGG GGGACATGGA GGGCTGCGGC CACTACCGTG TTGTGAAATA TGAACGGATT 
AAGTTCCTGG TCATTGCCCT GAAGAACTCC GTGGAGGTTT ATGCCTGGGC TCCCAAACCC 
TACCACAAAT TCATGGCCTT CAAGTCCTTT GCTGACCTCC CTCACCGCCC TCTACTGGTG 
GACCTGACAG TAGAGGAGGG ACAGCGGCTC AAGGTCATCT ATGGCTCCAG TGCTGGCTTC 
CATGCTGTGG ATGTTGATTC TGGGAACAGC TATGACATCT ACATCCCTGT ACATATCCAG 
AGCCAGATCA CACCCCACGC CATCATCTTC CTCCCCAACA CTGATGGCAT GGAGATGCTG 
CTGTGCTATG AAGATGAGGG TGTCTATGTC AACACTTACG GGCGGATCAT CAAGGATGTG 
GTGCTGCAGT GGGGAGAGAT GCCCACCTCT GTGGCCTACA TCTGCTCCAA CCAGATAATG 
GGCTGGGGTG AGAAGGCCAT AGAGATCCGC TCTGTGGAGA CAGGCCACCT AGATGGGGTC 
TTCATGCACA AACGAGCCCA GAGGCTCAAG TTCCTGTGTG AGCGCAATGA CAAGGTGTTT 
TTTGCCTCTG TCCGCTCTGG AGGAAGCAGC CAAGTTTACT TTATGACTCT GAACCGTAAC 
TGCATCATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533744.1
CDS	395..4432
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533681.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533681.3

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG 
CAGTCAGAGC GGCTGCAGAG ACAGCTGCAG CAGGAGCACG CCTACCTCAA GTCCCTGCAG 
CAGCAGCAGC AGCAGCAGCA GCTCCAGAAG CAGCAGCAGC AGCAGCAGCA GATCCTGCCT 
GGAGACAGGA AGCCCCTGTA TCATTACGGT CGGGGCATTA ATCCTGCTGA CAAGCCAGCA 
TGGGCCCGCG AGGTGGAAGA GAGAGCACGG ATGAACAAGC AGCAGAACTC TCCCTTGGCG 
AAGGCGAAGC CAAGCAGTGC GGGGCCAGAG CCCCCCATCT CCCAGGCCTC TCCTAGCCCC 
CCAGGACCTC TTTCCCAGAC TCCTCCTATG CAGAGGCCTG TGGAGCCCCA GGAAGGACCG 
CACAAGAGCC TGGTGGCACA CCGGGTCCCA CTGAAGCCAT ATGCAGCACC TGTACCCCGA 
TCCCAGTCCC TGCAGGACCA GCCGACTCGA AACCTGGCTG CCTTCCCAGC CTCCCACGAC 
CCTGACCCTG CTGCTGTCCC TACACCCACT GCCACACCCA GTGCCCGAGG AGCTGTCATC 
CGCCAGAATT CAGACCCCAC CTCTGAAGGG CCAGGGCCTA GCCCAAACCC TCCATCCTGG 
GTTCGGCCTG ATAATGAGGC TCCACCTAAG GTTCCACAGA GGACCTCTTC TATCGCCACT 
GCCCTTAACA CCAGTGGGGC CGGAGGGTCC CGGCCAGCTC AGGCTGTCCG TGCCAGACCT 
CGCAGTAACT CCGCCTGGCA AATCTATCTG CAGAGGCGGG CAGAGCGGGG CACCCCCAAG 
CCTCCCGGGC CCCCAGCTCA GCCCCCTGGC CCGCCCAACG CCTCTAGTAA CCCTGACCTC 
AGGAGGAGTG ACCCTGGCTG GGAGCGCTCA GACAGTGTCC TCCCGGCCTC CCACGGCCAC 
CTCCCTCAGG CTGGCTCCTT GGAGCGGAAC CGAAACCGTG TGGGAGCCTC CACAAAACTG 
GATAGCTCTC CAGTGCTCTC CCCTGGGAAC AAAGCCAAGC CTGAAGACCA CCGCTCAAGG 
CCAGGCCGGC CCGCAAGCTA TAAGCGAGCA ATTGGCGAGG ACTTTGTGTT GCTCAAAGAG 
CGGACTCTGG ATGAGGCCCC TAAGCCTCCC AAGAAGGCCA TGGACTACTC CTCATCCAGT 
GAGGAGGTGG AAAGCAGTGA AGAGGAGGAG GAGGAAGGCG ATGGGGAGCC GTCAGAGGGG 
AGCAGAGACA CTCCCGGGGG CCGCAGTGAT GGTGATACAG ACAGCGTCAG CACCATGGTG 
GTTCATGATG TTGAGGAGAT ATCCGGGACC CAGCCCTCAT ATGGCGGCGG CACCATGGTG 
GTCCAGCGTA CTCCTGAAGA GGAACGAAGC CTGCTGCTTG CTGATAGCAA TGGCTACACA 
AACCTGCCTG ATGTGGTCCA GCCCAGCCAC TCACCTACTG AGAACAGCAA AGGTCAAAGC 
CCTCCAACAA AGGATGGAGG CAGTGATTAC CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA 
AAGAGCTCAT TCACCATGTT TGTGGATCTA GGGATCTACC AGCCTGGAGG CAGTGGGGAC 
ACCATCCCTA TCACAGCCCT AGTGGGTGGA GAAGGTGGTC GCCTTGATCA ACTGCAGTTC 
GATGTGAGGA AGGGCTCTGT GGTCAACGTC AATCCCACCA ACACCCGAGC TCATAGTGAA 
ACTCCTGAAA TTCGCAAGTA CAAGAAGCGA TTCAACTCAG AGATCCTATG TGCAGCTCTC 
TGGGGGGTCA ACCTCCTAGT GGGCACAGAG AATGGGCTGA TGTTGCTGGA CCGAAGTGGG 
CAGGGCAAGG TGTATGGACT TATTGGGCGA CGACGCTTCC AGCAAATGGA TGTCTTAGAA 
GGGCTCAACT TGCTCATCAC CATCTCAGGG AAAAGGAACA AACTGCGGGT ATATTACCTG 
TCCTGGCTTC GGAACAAGAT CCTACACAAT GACCCAGAGG TGGAAAAGAA GCAGGGGTGG 
ACCACCGTGG GGGACATGGA GGGCTGCGGC CACTACCGTG TTGTGAAATA TGAACGGATT 
AAGTTCCTGG TCATTGCCCT GAAGAACTCC GTGGAGGTTT ATGCCTGGGC TCCCAAACCC 
TACCACAAAT TCATGGCCTT CAAGTCCTTT GCTGACCTCC CTCACCGCCC TCTACTGGTG 
GACCTGACAG TAGAGGAGGG ACAGCGGCTC AAGGTCATCT ATGGCTCCAG TGCTGGCTTC 
CATGCTGTGG ATGTTGATTC TGGGAACAGC TATGACATCT ACATCCCTGT ACATATCCAG 
AGCCAGATCA CACCCCACGC CATCATCTTC CTCCCCAACA CTGATGGCAT GGAGATGCTG 
CTGTGCTATG AAGATGAGGG TGTCTATGTC AACACTTACG GGCGGATCAT CAAGGATGTG 
GTGCTGCAGT GGGGAGAGAT GCCCACCTCT GTGGCCTACA TCTGCTCCAA CCAGATAATG 
GGCTGGGGTG AGAAGGCCAT AGAGATCCGC TCTGTGGAGA CAGGCCACCT AGATGGGGTC 
TTCATGCACA AACGAGCCCA GAGGCTCAAG TTCCTGTGTG AGCGCAATGA CAAGGTGTTT 
TTTGCCTCTG TCCGCTCTGG AGGAAGCAGC CAAGTTTACT TTATGACTCT GAACCGTAAC 
TGCATCATGA ACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_006533682.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006533745.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu46570
Clone ID Related Accession (Same CDS sequence)	XM_006533682.3 , XM_006533682.4
Accession Version	XM_006533682.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006533682.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006533745.1
CDS	397..4410
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_006533682.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006533682.3

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGC 
AGTGATGGTG ATACAGACAG CGTCAGCACC ATGGTGGTTC ATGATGTTGA GGAGATATCC 
GGGACCCAGC CCTCATATGG CGGCGGCACC ATGGTGGTCC AGCGTACTCC TGAAGAGGAA 
CGAAGCCTGC TGCTTGCTGA TAGCAATGGC TACACAAACC TGCCTGATGT GGTCCAGCCC 
AGCCACTCAC CTACTGAGAA CAGCAAAGGT CAAAGCCCTC CAACAAAGGA TGGAGGCAGT 
GATTACCAGT CTCGTGGGCT GGTAAAGGCC CCAGGAAAGA GCTCATTCAC CATGTTTGTG 
GATCTAGGGA TCTACCAGCC TGGAGGCAGT GGGGACACCA TCCCTATCAC AGCCCTAGTG 
GGTGGAGAAG GTGGTCGCCT TGATCAACTG CAGTTCGATG TGAGGAAGGG CTCTGTGGTC 
AACGTCAATC CCACCAACAC CCGAGCTCAT AGTGAAACTC CTGAAATTCG CAAGTACAAG 
AAGCGATTCA ACTCAGAGAT CCTATGTGCA GCTCTCTGGG GGGTCAACCT CCTAGTGGGC 
ACAGAGAATG GGCTGATGTT GCTGGACCGA AGTGGGCAGG GCAAGGTGTA TGGACTTATT 
GGGCGACGAC GCTTCCAGCA AATGGATGTC TTAGAAGGGC TCAACTTGCT CATCACCATC 
TCAGGGAAAA GGAACAAACT GCGGGTATAT TACCTGTCCT GGCTTCGGAA CAAGATCCTA 
CACAATGACC CAGAGGTGGA AAAGAAGCAG GGGTGGACCA CCGTGGGGGA CATGGAGGGC 
TGCGGCCACT ACCGTGTTGT GAAATATGAA CGGATTAAGT TCCTGGTCAT TGCCCTGAAG 
AACTCCGTGG AGGTTTATGC CTGGGCTCCC AAACCCTACC ACAAATTCAT GGCCTTCAAG 
TCCTTTGCTG ACCTCCCTCA CCGCCCTCTA CTGGTGGACC TGACAGTAGA GGAGGGACAG 
CGGCTCAAGG TCATCTATGG CTCCAGTGCT GGCTTCCATG CTGTGGATGT TGATTCTGGG 
AACAGCTATG ACATCTACAT CCCTGTACAT ATCCAGAGCC AGATCACACC CCACGCCATC 
ATCTTCCTCC CCAACACTGA TGGCATGGAG ATGCTGCTGT GCTATGAAGA TGAGGGTGTC 
TATGTCAACA CTTACGGGCG GATCATCAAG GATGTGGTGC TGCAGTGGGG AGAGATGCCC 
ACCTCTGTGG CCTACATCTG CTCCAACCAG ATAATGGGCT GGGGTGAGAA GGCCATAGAG 
ATCCGCTCTG TGGAGACAGG CCACCTAGAT GGGGTCTTCA TGCACAAACG AGCCCAGAGG 
CTCAAGTTCC TGTGTGAGCG CAATGACAAG GTGTTTTTTG CCTCTGTCCG CTCTGGAGGA 
AGCAGCCAAG TTTACTTTAT GACTCTGAAC CGTAACTGCA TCATGAACTG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_017314645.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170134.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.

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CloneID	OMu77971
Clone ID Related Accession (Same CDS sequence)	XM_017314645.2 , XM_017314645.1
Accession Version	XM_017314645.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGT 
GATGGTGATA CAGACAGCGT CAGCACCATG GTGGTTCATG ATGTTGAGGA GATATCCGGG 
ACCCAGCCCT CATATGGCGG CGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAACGA 
AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CTGATGTGGT CCAGCCCAGC 
CACTCACCTA CTGAGAACAG CAAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCAGTGAT 
TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGCT CATTCACCAT GTTTGTGGAT 
CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GACACCATCC CTATCACAGC CCTAGTGGGT 
GGAGAAGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC 
GTCAATCCCA CCAACACCCG AGCTCATAGT GAAACTCCTG AAATTCGCAA GTACAAGAAG 
CGATTCAACT CAGAGATCCT ATGTGCAGCT CTCTGGGGGG TCAACCTCCT AGTGGGCACA 
GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AGGTGTATGG ACTTATTGGG 
CGACGACGCT TCCAGCAAAT GGATGTCTTA GAAGGGCTCA ACTTGCTCAT CACCATCTCA 
GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAACAA GATCCTACAC 
AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC 
GGCCACTACC GTGTTGTGAA ATATGAACGG ATTAAGTTCC TGGTCATTGC CCTGAAGAAC 
TCCGTGGAGG TTTATGCCTG GGCTCCCAAA CCCTACCACA AATTCATGGC CTTCAAGTCC 
TTTGCTGACC TCCCTCACCG CCCTCTACTG GTGGACCTGA CAGTAGAGGA GGGACAGCGG 
CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA TTCTGGGAAC 
AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAGA TCACACCCCA CGCCATCATC 
TTCCTCCCCA ACACTGATGG CATGGAGATG CTGCTGTGCT ATGAAGATGA GGGTGTCTAT 
GTCAACACTT ACGGGCGGAT CATCAAGGAT GTGGTGCTGC AGTGGGGAGA GATGCCCACC 
TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC 
CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC 
AAGTTCCTGT GTGAGCGCAA TGACAAGGTG TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC 
AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170134.1
CDS	401..4411
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPASYKRAIGEDFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017314645.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314645.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AAGCTATAAG 
CGAGCAATTG GCGAGGACTT TGTGTTGCTC AAAGAGCGGA CTCTGGATGA GGCCCCTAAG 
CCTCCCAAGA AGGCCATGGA CTACTCCTCA TCCAGTGAGG AGGTGGAAAG CAGTGAAGAG 
GAGGAGGAGG AAGGCGATGG GGAGCCGTCA GAGGGGAGCA GAGACACTCC CGGGGGCCGT 
GATGGTGATA CAGACAGCGT CAGCACCATG GTGGTTCATG ATGTTGAGGA GATATCCGGG 
ACCCAGCCCT CATATGGCGG CGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAACGA 
AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CTGATGTGGT CCAGCCCAGC 
CACTCACCTA CTGAGAACAG CAAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCAGTGAT 
TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGCT CATTCACCAT GTTTGTGGAT 
CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GACACCATCC CTATCACAGC CCTAGTGGGT 
GGAGAAGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC 
GTCAATCCCA CCAACACCCG AGCTCATAGT GAAACTCCTG AAATTCGCAA GTACAAGAAG 
CGATTCAACT CAGAGATCCT ATGTGCAGCT CTCTGGGGGG TCAACCTCCT AGTGGGCACA 
GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AGGTGTATGG ACTTATTGGG 
CGACGACGCT TCCAGCAAAT GGATGTCTTA GAAGGGCTCA ACTTGCTCAT CACCATCTCA 
GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAACAA GATCCTACAC 
AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC 
GGCCACTACC GTGTTGTGAA ATATGAACGG ATTAAGTTCC TGGTCATTGC CCTGAAGAAC 
TCCGTGGAGG TTTATGCCTG GGCTCCCAAA CCCTACCACA AATTCATGGC CTTCAAGTCC 
TTTGCTGACC TCCCTCACCG CCCTCTACTG GTGGACCTGA CAGTAGAGGA GGGACAGCGG 
CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA TTCTGGGAAC 
AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAGA TCACACCCCA CGCCATCATC 
TTCCTCCCCA ACACTGATGG CATGGAGATG CTGCTGTGCT ATGAAGATGA GGGTGTCTAT 
GTCAACACTT ACGGGCGGAT CATCAAGGAT GTGGTGCTGC AGTGGGGAGA GATGCCCACC 
TCTGTGGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC 
CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC 
AAGTTCCTGT GTGAGCGCAA TGACAAGGTG TTTTTTGCCT CTGTCCGCTC TGGAGGAAGC 
AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mink1 ( XM_017314646.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170135.1 Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.

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CloneID	OMu77972
Clone ID Related Accession (Same CDS sequence)	XM_017314646.2 , XM_017314646.1
Accession Version	XM_017314646.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	misshapen-like kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AGACTTTGTG 
TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAC 
TCCTCATCCA GTGAGGAGGT GGAAAGCAGT GAAGAGGAGG AGGAGGAAGG CGATGGGGAG 
CCGTCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGATAC AGACAGCGTC 
AGCACCATGG TGGTTCATGA TGTTGAGGAG ATATCCGGGA CCCAGCCCTC ATATGGCGGC 
GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAACGAA GCCTGCTGCT TGCTGATAGC 
AATGGCTACA CAAACCTGCC TGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC 
AAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCAGTGATT ACCAGTCTCG TGGGCTGGTA 
AAGGCCCCAG GAAAGAGCTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA 
GGCAGTGGGG ACACCATCCC TATCACAGCC CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT 
CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACCCGA 
GCTCATAGTG AAACTCCTGA AATTCGCAAG TACAAGAAGC GATTCAACTC AGAGATCCTA 
TGTGCAGCTC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG 
GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG 
GATGTCTTAG AAGGGCTCAA CTTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG 
GTATATTACC TGTCCTGGCT TCGGAACAAG ATCCTACACA ATGACCCAGA GGTGGAAAAG 
AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTACCG TGTTGTGAAA 
TATGAACGGA TTAAGTTCCT GGTCATTGCC CTGAAGAACT CCGTGGAGGT TTATGCCTGG 
GCTCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC 
CCTCTACTGG TGGACCTGAC AGTAGAGGAG GGACAGCGGC TCAAGGTCAT CTATGGCTCC 
AGTGCTGGCT TCCATGCTGT GGATGTTGAT TCTGGGAACA GCTATGACAT CTACATCCCT 
GTACATATCC AGAGCCAGAT CACACCCCAC GCCATCATCT TCCTCCCCAA CACTGATGGC 
ATGGAGATGC TGCTGTGCTA TGAAGATGAG GGTGTCTATG TCAACACTTA CGGGCGGATC 
ATCAAGGATG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTGGCCTA CATCTGCTCC 
AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC 
CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT 
GACAAGGTGT TTTTTGCCTC TGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT 
CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170135.1
CDS	396..4385
Translation	MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFTDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYIRHRLEEEQRQLEILQQQLLQEQALLLEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQQQILPGDRKPLYHYGRGINPADKPAWAREVEERARMNKQQNSPLAKAKPSSAGPEPPISQASPSPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAAVPTPTATPSARGAVIRQNSDPTSEGPGPSPNPPSWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRASNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRNRVGASTKLDSSPVLSPGNKAKPEDHRSRPGRPADFVLLKERTLDEAPKPPKKAMDYSSSSEEVESSEEEEEEGDGEPSEGSRDTPGGRSDGDTDSVSTMVVHDVEEISGTQPSYGGGTMVVQRTPEEERSLLLADSNGYTNLPDVVQPSHSPTENSKGQSPPTKDGGSDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDTIPITALVGGEGGRLDQLQFDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKNSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

Target ORF information:

RefSeq Version	XM_017314646.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314646.1

ORF Insert Sequence:

ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCTGC CCTGCGGGAC 
CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTATAC 
AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG 
GATGAGGAGG AAGAGATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC 
AATATTGCCA CCTACTATGG GGCCTTTATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG 
CTCTGGCTGG TGATGGAGTT CTGCGGTGCT GGTTCAGTGA CCGACCTGGT AAAGAACACA 
AAAGGGAACG CACTGAAGGA GGATTGCATT GCTTACATCT GCAGGGAGAT TCTCAGGGGT 
CTTGCCCATC TCCATGCCCA CAAGGTGATC CACAGAGATA TCAAGGGACA AAATGTGCTG 
CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC 
ACTGTGGGCA GGCGGAACAC TTTCATTGGA ACCCCATACT GGATGGCTCC AGAAGTCATT 
GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA 
ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG 
GCCCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG 
AAGTTCACTG ACTTCATCGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACC 
GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC 
CAGCTCAAGG ACCACATCGA CCGCTCGCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAG 
TATGAGTACA GCGGCAGTGA GGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGC 
TCCATCATGA ATGTGCCCGG TGAGTCCACA CTGCGCAGAG AATTCCTCAG ACTCCAGCAG 
GAGAATAAGA GCAACTCTGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA ACAGCAGCAG 
CGGGACCCGG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG 
CAGAAGGAGG AGCGGCGACG TGTGGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGTAAG 
CTACAAGAGA AGGAGCAGCA GCGGCGATTG GAAGACATGC AAGCCCTACG ACGAGAGGAA 
GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATCCGTC ACAGGCTAGA GGAGGAGCAG 
CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC 
AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAGCGGC TGCAGAGACA GCTGCAGCAG 
GAGCACGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAGC AGCAGCAGCT CCAGAAGCAG 
CAGCAGCAGC AGCAGCAGAT CCTGCCTGGA GACAGGAAGC CCCTGTATCA TTACGGTCGG 
GGCATTAATC CTGCTGACAA GCCAGCATGG GCCCGCGAGG TGGAAGAGAG AGCACGGATG 
AACAAGCAGC AGAACTCTCC CTTGGCGAAG GCGAAGCCAA GCAGTGCGGG GCCAGAGCCC 
CCCATCTCCC AGGCCTCTCC TAGCCCCCCA GGACCTCTTT CCCAGACTCC TCCTATGCAG 
AGGCCTGTGG AGCCCCAGGA AGGACCGCAC AAGAGCCTGG TGGCACACCG GGTCCCACTG 
AAGCCATATG CAGCACCTGT ACCCCGATCC CAGTCCCTGC AGGACCAGCC GACTCGAAAC 
CTGGCTGCCT TCCCAGCCTC CCACGACCCT GACCCTGCTG CTGTCCCTAC ACCCACTGCC 
ACACCCAGTG CCCGAGGAGC TGTCATCCGC CAGAATTCAG ACCCCACCTC TGAAGGGCCA 
GGGCCTAGCC CAAACCCTCC ATCCTGGGTT CGGCCTGATA ATGAGGCTCC ACCTAAGGTT 
CCACAGAGGA CCTCTTCTAT CGCCACTGCC CTTAACACCA GTGGGGCCGG AGGGTCCCGG 
CCAGCTCAGG CTGTCCGTGC CAGTAACCCT GACCTCAGGA GGAGTGACCC TGGCTGGGAG 
CGCTCAGACA GTGTCCTCCC GGCCTCCCAC GGCCACCTCC CTCAGGCTGG CTCCTTGGAG 
CGGAACCGAA ACCGTGTGGG AGCCTCCACA AAACTGGATA GCTCTCCAGT GCTCTCCCCT 
GGGAACAAAG CCAAGCCTGA AGACCACCGC TCAAGGCCAG GCCGGCCCGC AGACTTTGTG 
TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAC 
TCCTCATCCA GTGAGGAGGT GGAAAGCAGT GAAGAGGAGG AGGAGGAAGG CGATGGGGAG 
CCGTCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGATAC AGACAGCGTC 
AGCACCATGG TGGTTCATGA TGTTGAGGAG ATATCCGGGA CCCAGCCCTC ATATGGCGGC 
GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAACGAA GCCTGCTGCT TGCTGATAGC 
AATGGCTACA CAAACCTGCC TGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC 
AAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCAGTGATT ACCAGTCTCG TGGGCTGGTA 
AAGGCCCCAG GAAAGAGCTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA 
GGCAGTGGGG ACACCATCCC TATCACAGCC CTAGTGGGTG GAGAAGGTGG TCGCCTTGAT 
CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACCCGA 
GCTCATAGTG AAACTCCTGA AATTCGCAAG TACAAGAAGC GATTCAACTC AGAGATCCTA 
TGTGCAGCTC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG 
GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG 
GATGTCTTAG AAGGGCTCAA CTTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG 
GTATATTACC TGTCCTGGCT TCGGAACAAG ATCCTACACA ATGACCCAGA GGTGGAAAAG 
AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTACCG TGTTGTGAAA 
TATGAACGGA TTAAGTTCCT GGTCATTGCC CTGAAGAACT CCGTGGAGGT TTATGCCTGG 
GCTCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC 
CCTCTACTGG TGGACCTGAC AGTAGAGGAG GGACAGCGGC TCAAGGTCAT CTATGGCTCC 
AGTGCTGGCT TCCATGCTGT GGATGTTGAT TCTGGGAACA GCTATGACAT CTACATCCCT 
GTACATATCC AGAGCCAGAT CACACCCCAC GCCATCATCT TCCTCCCCAA CACTGATGGC 
ATGGAGATGC TGCTGTGCTA TGAAGATGAG GGTGTCTATG TCAACACTTA CGGGCGGATC 
ATCAAGGATG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTGGCCTA CATCTGCTCC 
AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC 
CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGCAAT 
GACAAGGTGT TTTTTGCCTC TGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT 
CTGAACCGTA ACTGCATCAT GAACTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Mink1
Entrez Gene ID	50932
Full Name	misshapen-like kinase 1 (zebrafish)
Synonyms	B55,MINK,Map4k6,Ysk2
General protein information	Preferred Names misshapen-like kinase 1 (zebrafish) Names misshapen-like kinase 1 GCK family kinase MiNK MAPK/ERK kinase kinase kinase 6 MEK kinase kinase 6 MEKKK 6 Misshapen/NIKs-related kinase Yeast Sps1/Ste20-related kinase 2 mink1 misshapen/NIK-related kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B3

Mus musculus (house mouse)	Mink1	NP_001039429.1
Canis lupus familiaris (dog)	MINK1	XP_005619987.1
Homo sapiens (human)	MINK1	NP_722549.2
Bos taurus (cattle)	MINK1	NP_001092846.1
Rattus norvegicus (Norway rat)	Mink1	NP_001258065.1
Xenopus tropicalis (tropical clawed frog)	mink1	NP_001072739.1
	MINK1	XP_001117764.2
Pan troglodytes (chimpanzee)	MINK1	XP_511292.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Mink1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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