MINK1 cDNA ORF clone, Homo sapiens(human)

The following MINK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MINK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09415 NM_153827.5
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu09319 NM_015716.5
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
OHu09501 NM_001024937.4
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu19321 NM_170663.5
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu49858 NM_001321236.2
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu49849 XM_006721531.2
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu49850 XM_006721532.2
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu49855 XM_006721536.2
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu67500 XM_011523906.1
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu86902 XM_017024704.1
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu86903 XM_017024705.1
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu86904 XM_017024706.1
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu86905 XM_017024707.2
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu86906 XM_017024708.1
Latest version!
Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu86905 XM_017024707.1 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu09415 NM_153827.4 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $384.30
$549.00
OHu09501 NM_001024937.3 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu49858 NM_001321236.1 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu09319 NM_015716.4 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
OHu19321 NM_170663.4 Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu09415
    Clone ID Related Accession (Same CDS sequence) NM_153827.5 , NM_153827.4
    Accession Version NM_153827.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, BC034673.1, AB035698.1, AB041926.1 and AL157418.1. On Nov 22, 2018 this sequence version replaced NM_153827.4. Transcript Variant: This variant (3) includes an additional in-frame exon, compared to variant 1, resulting in the longest isoform (3) which has a unique internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355280.11/ ENSP00000347427.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722549.2
    CDS245..4243
    Translation

    Target ORF information:

    RefSeq Version NM_153827.5
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153827.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09319
    Clone ID Related Accession (Same CDS sequence) NM_015716.4 , NM_015716.5
    Accession Version NM_015716.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AB035698.1, AB041926.1 and AL157418.1. On Sep 23, 2009 this sequence version replaced NM_015716.3. Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAG ACTTTGTGTT GCTGAAAGAG CGGACTCTGG ACGAGGCCCC TCGGCCTCCC
    AAGAAGGCCA TGGACTACTC GTCGTCCAGC GAGGAGGTGG AAAGCAGTGA GGACGACGAG
    GAGGAAGGCG AAGGCGGGCC AGCAGAGGGG AGCAGAGATA CCCCTGGGGG CCGCAGCGAT
    GGGGATACAG ACAGCGTCAG CACCATGGTG GTCCACGACG TCGAGGAGAT CACCGGGACC
    CAGCCCCCAT ACGGGGGCGG CACCATGGTG GTCCAGCGCA CCCCTGAAGA GGAGCGGAAC
    CTGCTGCATG CTGACAGCAA TGGGTACACA AACCTGCCTG ACGTGGTCCA GCCCAGCCAC
    TCACCCACCG AGAACAGCAA AGGCCAAAGC CCACCCTCGA AGGATGGGAG TGGTGACTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCTGGC AAGAGCTCGT TCACGATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAC AGCATCCCCA TCACAGCCCT AGTGGGTGGA
    GAGGGCACTC GGCTCGACCA GCTGCAGTAC GACGTGAGGA AGGGTTCTGT GGTCAACGTG
    AATCCCACCA ACACCCGGGC CCACAGTGAG ACCCCTGAGA TCCGGAAGTA CAAGAAGCGA
    TTCAACTCCG AGATCCTCTG TGCAGCCCTT TGGGGGGTCA ACCTGCTGGT GGGCACGGAG
    AACGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAGG TGTATGGACT CATTGGGCGG
    CGACGCTTCC AGCAGATGGA TGTGCTGGAG GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT GTATTACCTG TCCTGGCTCC GGAACAAGAT TCTGCACAAT
    GACCCAGAAG TGGAGAAGAA GCAGGGCTGG ACCACCGTGG GGGACATGGA GGGCTGCGGG
    CACTACCGTG TTGTGAAATA CGAGCGGATT AAGTTCCTGG TCATCGCCCT CAAGAGCTCC
    GTGGAGGTGT ATGCCTGGGC CCCCAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCCGACCTCC CCCACCGCCC TCTGCTGGTC GACCTGACAG TAGAGGAGGG GCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTCGACTC GGGGAACAGC
    TATGACATCT ACATCCCTGT GCACATCCAG AGCCAGATCA CGCCCCATGC CATCATCTTC
    CTCCCCAACA CCGACGGCAT GGAGATGCTG CTGTGCTACG AGGACGAGGG TGTCTACGTC
    AACACGTACG GGCGCATCAT TAAGGATGTG GTGCTGCAGT GGGGGGAGAT GCCTACTTCT
    GTGGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAAGCCAT TGAGATCCGC
    TCTGTGGAGA CGGGCCACCT CGACGGGGTC TTCATGCACA AACGAGCTCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA CAAGGTGTTT TTTGCCTCAG TCCGCTCTGG GGGCAGCAGC
    CAAGTTTACT TCATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056531.1
    CDS245..4132
    Translation

    Target ORF information:

    RefSeq Version NM_015716.4
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015716.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAG ACTTTGTGTT GCTGAAAGAG CGGACTCTGG ACGAGGCCCC TCGGCCTCCC
    AAGAAGGCCA TGGACTACTC GTCGTCCAGC GAGGAGGTGG AAAGCAGTGA GGACGACGAG
    GAGGAAGGCG AAGGCGGGCC AGCAGAGGGG AGCAGAGATA CCCCTGGGGG CCGCAGCGAT
    GGGGATACAG ACAGCGTCAG CACCATGGTG GTCCACGACG TCGAGGAGAT CACCGGGACC
    CAGCCCCCAT ACGGGGGCGG CACCATGGTG GTCCAGCGCA CCCCTGAAGA GGAGCGGAAC
    CTGCTGCATG CTGACAGCAA TGGGTACACA AACCTGCCTG ACGTGGTCCA GCCCAGCCAC
    TCACCCACCG AGAACAGCAA AGGCCAAAGC CCACCCTCGA AGGATGGGAG TGGTGACTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCTGGC AAGAGCTCGT TCACGATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAC AGCATCCCCA TCACAGCCCT AGTGGGTGGA
    GAGGGCACTC GGCTCGACCA GCTGCAGTAC GACGTGAGGA AGGGTTCTGT GGTCAACGTG
    AATCCCACCA ACACCCGGGC CCACAGTGAG ACCCCTGAGA TCCGGAAGTA CAAGAAGCGA
    TTCAACTCCG AGATCCTCTG TGCAGCCCTT TGGGGGGTCA ACCTGCTGGT GGGCACGGAG
    AACGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAGG TGTATGGACT CATTGGGCGG
    CGACGCTTCC AGCAGATGGA TGTGCTGGAG GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT GTATTACCTG TCCTGGCTCC GGAACAAGAT TCTGCACAAT
    GACCCAGAAG TGGAGAAGAA GCAGGGCTGG ACCACCGTGG GGGACATGGA GGGCTGCGGG
    CACTACCGTG TTGTGAAATA CGAGCGGATT AAGTTCCTGG TCATCGCCCT CAAGAGCTCC
    GTGGAGGTGT ATGCCTGGGC CCCCAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCCGACCTCC CCCACCGCCC TCTGCTGGTC GACCTGACAG TAGAGGAGGG GCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTCGACTC GGGGAACAGC
    TATGACATCT ACATCCCTGT GCACATCCAG AGCCAGATCA CGCCCCATGC CATCATCTTC
    CTCCCCAACA CCGACGGCAT GGAGATGCTG CTGTGCTACG AGGACGAGGG TGTCTACGTC
    AACACGTACG GGCGCATCAT TAAGGATGTG GTGCTGCAGT GGGGGGAGAT GCCTACTTCT
    GTGGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAAGCCAT TGAGATCCGC
    TCTGTGGAGA CGGGCCACCT CGACGGGGTC TTCATGCACA AACGAGCTCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA CAAGGTGTTT TTTGCCTCAG TCCGCTCTGG GGGCAGCAGC
    CAAGTTTACT TCATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09319
    Clone ID Related Accession (Same CDS sequence) NM_015716.4 , NM_015716.5
    Accession Version NM_015716.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-06
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AB035698.1, AB041926.1 and AL157418.1. On May 31, 2019 this sequence version replaced NM_015716.4. Transcript Variant: This variant (1) represents the shortest transcript and encodes the shortest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB035698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAG ACTTTGTGTT GCTGAAAGAG CGGACTCTGG ACGAGGCCCC TCGGCCTCCC
    AAGAAGGCCA TGGACTACTC GTCGTCCAGC GAGGAGGTGG AAAGCAGTGA GGACGACGAG
    GAGGAAGGCG AAGGCGGGCC AGCAGAGGGG AGCAGAGATA CCCCTGGGGG CCGCAGCGAT
    GGGGATACAG ACAGCGTCAG CACCATGGTG GTCCACGACG TCGAGGAGAT CACCGGGACC
    CAGCCCCCAT ACGGGGGCGG CACCATGGTG GTCCAGCGCA CCCCTGAAGA GGAGCGGAAC
    CTGCTGCATG CTGACAGCAA TGGGTACACA AACCTGCCTG ACGTGGTCCA GCCCAGCCAC
    TCACCCACCG AGAACAGCAA AGGCCAAAGC CCACCCTCGA AGGATGGGAG TGGTGACTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCTGGC AAGAGCTCGT TCACGATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAC AGCATCCCCA TCACAGCCCT AGTGGGTGGA
    GAGGGCACTC GGCTCGACCA GCTGCAGTAC GACGTGAGGA AGGGTTCTGT GGTCAACGTG
    AATCCCACCA ACACCCGGGC CCACAGTGAG ACCCCTGAGA TCCGGAAGTA CAAGAAGCGA
    TTCAACTCCG AGATCCTCTG TGCAGCCCTT TGGGGGGTCA ACCTGCTGGT GGGCACGGAG
    AACGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAGG TGTATGGACT CATTGGGCGG
    CGACGCTTCC AGCAGATGGA TGTGCTGGAG GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT GTATTACCTG TCCTGGCTCC GGAACAAGAT TCTGCACAAT
    GACCCAGAAG TGGAGAAGAA GCAGGGCTGG ACCACCGTGG GGGACATGGA GGGCTGCGGG
    CACTACCGTG TTGTGAAATA CGAGCGGATT AAGTTCCTGG TCATCGCCCT CAAGAGCTCC
    GTGGAGGTGT ATGCCTGGGC CCCCAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCCGACCTCC CCCACCGCCC TCTGCTGGTC GACCTGACAG TAGAGGAGGG GCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTCGACTC GGGGAACAGC
    TATGACATCT ACATCCCTGT GCACATCCAG AGCCAGATCA CGCCCCATGC CATCATCTTC
    CTCCCCAACA CCGACGGCAT GGAGATGCTG CTGTGCTACG AGGACGAGGG TGTCTACGTC
    AACACGTACG GGCGCATCAT TAAGGATGTG GTGCTGCAGT GGGGGGAGAT GCCTACTTCT
    GTGGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAAGCCAT TGAGATCCGC
    TCTGTGGAGA CGGGCCACCT CGACGGGGTC TTCATGCACA AACGAGCTCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA CAAGGTGTTT TTTGCCTCAG TCCGCTCTGG GGGCAGCAGC
    CAAGTTTACT TCATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056531.1
    CDS245..4132
    Translation

    Target ORF information:

    RefSeq Version NM_015716.5
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015716.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAG ACTTTGTGTT GCTGAAAGAG CGGACTCTGG ACGAGGCCCC TCGGCCTCCC
    AAGAAGGCCA TGGACTACTC GTCGTCCAGC GAGGAGGTGG AAAGCAGTGA GGACGACGAG
    GAGGAAGGCG AAGGCGGGCC AGCAGAGGGG AGCAGAGATA CCCCTGGGGG CCGCAGCGAT
    GGGGATACAG ACAGCGTCAG CACCATGGTG GTCCACGACG TCGAGGAGAT CACCGGGACC
    CAGCCCCCAT ACGGGGGCGG CACCATGGTG GTCCAGCGCA CCCCTGAAGA GGAGCGGAAC
    CTGCTGCATG CTGACAGCAA TGGGTACACA AACCTGCCTG ACGTGGTCCA GCCCAGCCAC
    TCACCCACCG AGAACAGCAA AGGCCAAAGC CCACCCTCGA AGGATGGGAG TGGTGACTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCTGGC AAGAGCTCGT TCACGATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAC AGCATCCCCA TCACAGCCCT AGTGGGTGGA
    GAGGGCACTC GGCTCGACCA GCTGCAGTAC GACGTGAGGA AGGGTTCTGT GGTCAACGTG
    AATCCCACCA ACACCCGGGC CCACAGTGAG ACCCCTGAGA TCCGGAAGTA CAAGAAGCGA
    TTCAACTCCG AGATCCTCTG TGCAGCCCTT TGGGGGGTCA ACCTGCTGGT GGGCACGGAG
    AACGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAGG TGTATGGACT CATTGGGCGG
    CGACGCTTCC AGCAGATGGA TGTGCTGGAG GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT GTATTACCTG TCCTGGCTCC GGAACAAGAT TCTGCACAAT
    GACCCAGAAG TGGAGAAGAA GCAGGGCTGG ACCACCGTGG GGGACATGGA GGGCTGCGGG
    CACTACCGTG TTGTGAAATA CGAGCGGATT AAGTTCCTGG TCATCGCCCT CAAGAGCTCC
    GTGGAGGTGT ATGCCTGGGC CCCCAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCCGACCTCC CCCACCGCCC TCTGCTGGTC GACCTGACAG TAGAGGAGGG GCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTCGACTC GGGGAACAGC
    TATGACATCT ACATCCCTGT GCACATCCAG AGCCAGATCA CGCCCCATGC CATCATCTTC
    CTCCCCAACA CCGACGGCAT GGAGATGCTG CTGTGCTACG AGGACGAGGG TGTCTACGTC
    AACACGTACG GGCGCATCAT TAAGGATGTG GTGCTGCAGT GGGGGGAGAT GCCTACTTCT
    GTGGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAAGCCAT TGAGATCCGC
    TCTGTGGAGA CGGGCCACCT CGACGGGGTC TTCATGCACA AACGAGCTCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA CAAGGTGTTT TTTGCCTCAG TCCGCTCTGG GGGCAGCAGC
    CAAGTTTACT TCATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09415
    Clone ID Related Accession (Same CDS sequence) NM_153827.5 , NM_153827.4
    Accession Version NM_153827.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-09
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, BC034673.1, AB035698.1, AB041926.1 and AL157418.1. On Sep 23, 2009 this sequence version replaced NM_153827.3. Transcript Variant: This variant (3) includes an additional in-frame exon, compared to variant 1, resulting in the longest isoform (3) which has a unique internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034673.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_722549.2
    CDS245..4243
    Translation

    Target ORF information:

    RefSeq Version NM_153827.4
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_153827.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09501
    Clone ID Related Accession (Same CDS sequence) NM_001024937.4 , NM_001024937.3
    Accession Version NM_001024937.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-06
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AY775058.1, AL157418.1 and AB041926.1. On May 31, 2019 this sequence version replaced NM_001024937.3. Transcript Variant: This variant (4) lacks an alternate in-frame exon and contains another alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini and is longer compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY775058.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020108.1
    CDS245..4183
    Translation

    Target ORF information:

    RefSeq Version NM_001024937.4
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001024937.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu09501
    Clone ID Related Accession (Same CDS sequence) NM_001024937.4 , NM_001024937.3
    Accession Version NM_001024937.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AY775058.1, AL157418.1 and AB041926.1. On Sep 23, 2009 this sequence version replaced NM_001024937.2. Transcript Variant: This variant (4) lacks an alternate in-frame exon and contains another alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini and is longer compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY775058.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001020108.1
    CDS245..4183
    Translation

    Target ORF information:

    RefSeq Version NM_001024937.3
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001024937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGACC TCGCAGCAAC
    TCCGCCTGGC AAATCTATCT GCAAAGGCGG GCAGAGCGGG GCACCCCAAA GCCTCCAGGG
    CCCCCTGCTC AGCCCCCTGG CCCGCCCAAC GCCTCTAGTA ACCCCGACCT CAGGAGGAGC
    GACCCTGGCT GGGAACGCTC GGACAGCGTC CTTCCAGCCT CTCACGGGCA CCTCCCCCAG
    GCTGGCTCAC TGGAGCGGAA CCGCGTGGGA GTCTCCTCCA AACCGGACAG CTCCCCTGTG
    CTCTCCCCTG GGAATAAAGC CAAGCCCGAC GACCACCGCT CACGGCCAGG CCGGCCCGCA
    GACTTTGTGT TGCTGAAAGA GCGGACTCTG GACGAGGCCC CTCGGCCTCC CAAGAAGGCC
    ATGGACTACT CGTCGTCCAG CGAGGAGGTG GAAAGCAGTG AGGACGACGA GGAGGAAGGC
    GAAGGCGGGC CAGCAGAGGG GAGCAGAGAT ACCCCTGGGG GCCGCAGCGA TGGGGATACA
    GACAGCGTCA GCACCATGGT GGTCCACGAC GTCGAGGAGA TCACCGGGAC CCAGCCCCCA
    TACGGGGGCG GCACCATGGT GGTCCAGCGC ACCCCTGAAG AGGAGCGGAA CCTGCTGCAT
    GCTGACAGCA ATGGGTACAC AAACCTGCCT GACGTGGTCC AGCCCAGCCA CTCACCCACC
    GAGAACAGCA AAGGCCAAAG CCCACCCTCG AAGGATGGGA GTGGTGACTA CCAGTCTCGT
    GGGCTGGTAA AGGCCCCTGG CAAGAGCTCG TTCACGATGT TTGTGGATCT AGGGATCTAC
    CAGCCTGGAG GCAGTGGGGA CAGCATCCCC ATCACAGCCC TAGTGGGTGG AGAGGGCACT
    CGGCTCGACC AGCTGCAGTA CGACGTGAGG AAGGGTTCTG TGGTCAACGT GAATCCCACC
    AACACCCGGG CCCACAGTGA GACCCCTGAG ATCCGGAAGT ACAAGAAGCG ATTCAACTCC
    GAGATCCTCT GTGCAGCCCT TTGGGGGGTC AACCTGCTGG TGGGCACGGA GAACGGGCTG
    ATGTTGCTGG ACCGAAGTGG GCAGGGCAAG GTGTATGGAC TCATTGGGCG GCGACGCTTC
    CAGCAGATGG ATGTGCTGGA GGGGCTCAAC CTGCTCATCA CCATCTCAGG GAAAAGGAAC
    AAACTGCGGG TGTATTACCT GTCCTGGCTC CGGAACAAGA TTCTGCACAA TGACCCAGAA
    GTGGAGAAGA AGCAGGGCTG GACCACCGTG GGGGACATGG AGGGCTGCGG GCACTACCGT
    GTTGTGAAAT ACGAGCGGAT TAAGTTCCTG GTCATCGCCC TCAAGAGCTC CGTGGAGGTG
    TATGCCTGGG CCCCCAAACC CTACCACAAA TTCATGGCCT TCAAGTCCTT TGCCGACCTC
    CCCCACCGCC CTCTGCTGGT CGACCTGACA GTAGAGGAGG GGCAGCGGCT CAAGGTCATC
    TATGGCTCCA GTGCTGGCTT CCATGCTGTG GATGTCGACT CGGGGAACAG CTATGACATC
    TACATCCCTG TGCACATCCA GAGCCAGATC ACGCCCCATG CCATCATCTT CCTCCCCAAC
    ACCGACGGCA TGGAGATGCT GCTGTGCTAC GAGGACGAGG GTGTCTACGT CAACACGTAC
    GGGCGCATCA TTAAGGATGT GGTGCTGCAG TGGGGGGAGA TGCCTACTTC TGTGGCCTAC
    ATCTGCTCCA ACCAGATAAT GGGCTGGGGT GAGAAAGCCA TTGAGATCCG CTCTGTGGAG
    ACGGGCCACC TCGACGGGGT CTTCATGCAC AAACGAGCTC AGAGGCTCAA GTTCCTGTGT
    GAGCGGAATG ACAAGGTGTT TTTTGCCTCA GTCCGCTCTG GGGGCAGCAG CCAAGTTTAC
    TTCATGACTC TGAACCGTAA CTGCATCATG AACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19321
    Clone ID Related Accession (Same CDS sequence) NM_170663.4 , NM_170663.5
    Accession Version NM_170663.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AB041926.1 and AL157418.1. This sequence is a reference standard in the RefSeqGene project. On Sep 23, 2009 this sequence version replaced NM_170663.3. Transcript Variant: This variant (2) includes an additional in-frame exon, compared to variant 1, resulting in a longer isoform (2) that has a unique internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB041926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAA GCTATAAGCG AGCAATTGGT GAGGACTTTG TGTTGCTGAA AGAGCGGACT
    CTGGACGAGG CCCCTCGGCC TCCCAAGAAG GCCATGGACT ACTCGTCGTC CAGCGAGGAG
    GTGGAAAGCA GTGAGGACGA CGAGGAGGAA GGCGAAGGCG GGCCAGCAGA GGGGAGCAGA
    GATACCCCTG GGGGCCGCAG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733763.1
    CDS245..4156
    Translation

    Target ORF information:

    RefSeq Version NM_170663.4
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170663.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAA GCTATAAGCG AGCAATTGGT GAGGACTTTG TGTTGCTGAA AGAGCGGACT
    CTGGACGAGG CCCCTCGGCC TCCCAAGAAG GCCATGGACT ACTCGTCGTC CAGCGAGGAG
    GTGGAAAGCA GTGAGGACGA CGAGGAGGAA GGCGAAGGCG GGCCAGCAGA GGGGAGCAGA
    GATACCCCTG GGGGCCGCAG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19321
    Clone ID Related Accession (Same CDS sequence) NM_170663.4 , NM_170663.5
    Accession Version NM_170663.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, AB041926.1 and AL157418.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_170663.4. Transcript Variant: This variant (2) includes an additional in-frame exon, compared to variant 1, resulting in a longer isoform (2) that has a unique internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB041926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAA GCTATAAGCG AGCAATTGGT GAGGACTTTG TGTTGCTGAA AGAGCGGACT
    CTGGACGAGG CCCCTCGGCC TCCCAAGAAG GCCATGGACT ACTCGTCGTC CAGCGAGGAG
    GTGGAAAGCA GTGAGGACGA CGAGGAGGAA GGCGAAGGCG GGCCAGCAGA GGGGAGCAGA
    GATACCCCTG GGGGCCGCAG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733763.1
    CDS245..4156
    Translation

    Target ORF information:

    RefSeq Version NM_170663.5
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170663.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCAGAAC GTCTCCAGAG GCAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAAC AGCAGCAGCA GCTTCAGAAA CAGCAGCAGC AGCAGCTCCT GCCTGGGGAC
    AGGAAGCCCC TGTACCATTA TGGTCGGGGC ATGAATCCCG CTGACAAACC AGCCTGGGCC
    CGAGAGGTAG AAGAGAGAAC AAGGATGAAC AAGCAGCAGA ACTCTCCCTT GGCCAAGAGC
    AAGCCAGGCA GCACGGGGCC TGAGCCCCCC ATCCCCCAGG CCTCCCCAGG GCCCCCAGGA
    CCCCTTTCCC AGACTCCTCC TATGCAGAGG CCGGTGGAGC CCCAGGAGGG ACCGCACAAG
    AGCCTGGTGG CACACCGGGT CCCACTGAAG CCATATGCAG CACCTGTACC CCGATCCCAG
    TCCCTGCAGG ACCAGCCCAC CCGAAACCTG GCTGCCTTCC CAGCCTCCCA TGACCCCGAC
    CCTGCCATCC CCGCACCCAC TGCCACGCCC AGTGCCCGAG GAGCTGTCAT CCGCCAGAAT
    TCAGACCCCA CCTCTGAAGG ACCTGGCCCC AGCCCGAATC CCCCAGCCTG GGTCCGCCCA
    GATAACGAGG CCCCACCCAA GGTGCCTCAG AGGACCTCAT CTATCGCCAC TGCCCTTAAC
    ACCAGTGGGG CCGGAGGGTC CCGGCCAGCC CAGGCAGTCC GTGCCAGTAA CCCCGACCTC
    AGGAGGAGCG ACCCTGGCTG GGAACGCTCG GACAGCGTCC TTCCAGCCTC TCACGGGCAC
    CTCCCCCAGG CTGGCTCACT GGAGCGGAAC CGCGTGGGAG TCTCCTCCAA ACCGGACAGC
    TCCCCTGTGC TCTCCCCTGG GAATAAAGCC AAGCCCGACG ACCACCGCTC ACGGCCAGGC
    CGGCCCGCAA GCTATAAGCG AGCAATTGGT GAGGACTTTG TGTTGCTGAA AGAGCGGACT
    CTGGACGAGG CCCCTCGGCC TCCCAAGAAG GCCATGGACT ACTCGTCGTC CAGCGAGGAG
    GTGGAAAGCA GTGAGGACGA CGAGGAGGAA GGCGAAGGCG GGCCAGCAGA GGGGAGCAGA
    GATACCCCTG GGGGCCGCAG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49858
    Clone ID Related Accession (Same CDS sequence) NM_001321236.1 , NM_001321236.2
    Accession Version NM_001321236.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, BC094686.1, AB041926.1 and AL157418.1. On Mar 18, 2016 this sequence version replaced XM_011523908.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATACAA GCGGAAGCAG
    CTGGAGGAGC AGCGGCAGTC AGAACGTCTC CAGAGGCAGC TGCAGCAGGA GCATGCCTAC
    CTCAAGTCCC TGCAGCAGCA GCAACAGCAG CAGCAGCTTC AGAAACAGCA GCAGCAGCAG
    CTCCTGCCTG GGGACAGGAA GCCCCTGTAC CATTATGGTC GGGGCATGAA TCCCGCTGAC
    AAACCAGCCT GGGCCCGAGA GGTAGAAGAG AGAACAAGGA TGAACAAGCA GCAGAACTCT
    CCCTTGGCCA AGAGCAAGCC AGGCAGCACG GGGCCTGAGC CCCCCATCCC CCAGGCCTCC
    CCAGGGCCCC CAGGACCCCT TTCCCAGACT CCTCCTATGC AGAGGCCGGT GGAGCCCCAG
    GAGGGACCGC ACAAGAGCCT GGTGGCACAC CGGGTCCCAC TGAAGCCATA TGCAGCACCT
    GTACCCCGAT CCCAGTCCCT GCAGGACCAG CCCACCCGAA ACCTGGCTGC CTTCCCAGCC
    TCCCATGACC CCGACCCTGC CATCCCCGCA CCCACTGCCA CGCCCAGTGC CCGAGGAGCT
    GTCATCCGCC AGAATTCAGA CCCCACCTCT GAAGGACCTG GCCCCAGCCC GAATCCCCCA
    GCCTGGGTCC GCCCAGATAA CGAGGCCCCA CCCAAGGTGC CTCAGAGGAC CTCATCTATC
    GCCACTGCCC TTAACACCAG TGGGGCCGGA GGGTCCCGGC CAGCCCAGGC AGTCCGTGCC
    AGTAACCCCG ACCTCAGGAG GAGCGACCCT GGCTGGGAAC GCTCGGACAG CGTCCTTCCA
    GCCTCTCACG GGCACCTCCC CCAGGCTGGC TCACTGGAGC GGAACCGCGT GGGAGTCTCC
    TCCAAACCGG ACAGCTCCCC TGTGCTCTCC CCTGGGAATA AAGCCAAGCC CGACGACCAC
    CGCTCACGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTGA AAGAGCGGAC TCTGGACGAG
    GCCCCTCGGC CTCCCAAGAA GGCCATGGAC TACTCGTCGT CCAGCGAGGA GGTGGAAAGC
    AGTGAGGACG ACGAGGAGGA AGGCGAAGGC GGGCCAGCAG AGGGGAGCAG AGATACCCCT
    GGGGGCCGCA GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308165.1
    CDS526..4248
    Translation

    Target ORF information:

    RefSeq Version NM_001321236.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATACAA GCGGAAGCAG
    CTGGAGGAGC AGCGGCAGTC AGAACGTCTC CAGAGGCAGC TGCAGCAGGA GCATGCCTAC
    CTCAAGTCCC TGCAGCAGCA GCAACAGCAG CAGCAGCTTC AGAAACAGCA GCAGCAGCAG
    CTCCTGCCTG GGGACAGGAA GCCCCTGTAC CATTATGGTC GGGGCATGAA TCCCGCTGAC
    AAACCAGCCT GGGCCCGAGA GGTAGAAGAG AGAACAAGGA TGAACAAGCA GCAGAACTCT
    CCCTTGGCCA AGAGCAAGCC AGGCAGCACG GGGCCTGAGC CCCCCATCCC CCAGGCCTCC
    CCAGGGCCCC CAGGACCCCT TTCCCAGACT CCTCCTATGC AGAGGCCGGT GGAGCCCCAG
    GAGGGACCGC ACAAGAGCCT GGTGGCACAC CGGGTCCCAC TGAAGCCATA TGCAGCACCT
    GTACCCCGAT CCCAGTCCCT GCAGGACCAG CCCACCCGAA ACCTGGCTGC CTTCCCAGCC
    TCCCATGACC CCGACCCTGC CATCCCCGCA CCCACTGCCA CGCCCAGTGC CCGAGGAGCT
    GTCATCCGCC AGAATTCAGA CCCCACCTCT GAAGGACCTG GCCCCAGCCC GAATCCCCCA
    GCCTGGGTCC GCCCAGATAA CGAGGCCCCA CCCAAGGTGC CTCAGAGGAC CTCATCTATC
    GCCACTGCCC TTAACACCAG TGGGGCCGGA GGGTCCCGGC CAGCCCAGGC AGTCCGTGCC
    AGTAACCCCG ACCTCAGGAG GAGCGACCCT GGCTGGGAAC GCTCGGACAG CGTCCTTCCA
    GCCTCTCACG GGCACCTCCC CCAGGCTGGC TCACTGGAGC GGAACCGCGT GGGAGTCTCC
    TCCAAACCGG ACAGCTCCCC TGTGCTCTCC CCTGGGAATA AAGCCAAGCC CGACGACCAC
    CGCTCACGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTGA AAGAGCGGAC TCTGGACGAG
    GCCCCTCGGC CTCCCAAGAA GGCCATGGAC TACTCGTCGT CCAGCGAGGA GGTGGAAAGC
    AGTGAGGACG ACGAGGAGGA AGGCGAAGGC GGGCCAGCAG AGGGGAGCAG AGATACCCCT
    GGGGGCCGCA GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49858
    Clone ID Related Accession (Same CDS sequence) NM_001321236.1 , NM_001321236.2
    Accession Version NM_001321236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3723bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-06
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653105.1, BC094686.1, AB041926.1 and AL157418.1. On May 31, 2019 this sequence version replaced NM_001321236.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC094686.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATACAA GCGGAAGCAG
    CTGGAGGAGC AGCGGCAGTC AGAACGTCTC CAGAGGCAGC TGCAGCAGGA GCATGCCTAC
    CTCAAGTCCC TGCAGCAGCA GCAACAGCAG CAGCAGCTTC AGAAACAGCA GCAGCAGCAG
    CTCCTGCCTG GGGACAGGAA GCCCCTGTAC CATTATGGTC GGGGCATGAA TCCCGCTGAC
    AAACCAGCCT GGGCCCGAGA GGTAGAAGAG AGAACAAGGA TGAACAAGCA GCAGAACTCT
    CCCTTGGCCA AGAGCAAGCC AGGCAGCACG GGGCCTGAGC CCCCCATCCC CCAGGCCTCC
    CCAGGGCCCC CAGGACCCCT TTCCCAGACT CCTCCTATGC AGAGGCCGGT GGAGCCCCAG
    GAGGGACCGC ACAAGAGCCT GGTGGCACAC CGGGTCCCAC TGAAGCCATA TGCAGCACCT
    GTACCCCGAT CCCAGTCCCT GCAGGACCAG CCCACCCGAA ACCTGGCTGC CTTCCCAGCC
    TCCCATGACC CCGACCCTGC CATCCCCGCA CCCACTGCCA CGCCCAGTGC CCGAGGAGCT
    GTCATCCGCC AGAATTCAGA CCCCACCTCT GAAGGACCTG GCCCCAGCCC GAATCCCCCA
    GCCTGGGTCC GCCCAGATAA CGAGGCCCCA CCCAAGGTGC CTCAGAGGAC CTCATCTATC
    GCCACTGCCC TTAACACCAG TGGGGCCGGA GGGTCCCGGC CAGCCCAGGC AGTCCGTGCC
    AGTAACCCCG ACCTCAGGAG GAGCGACCCT GGCTGGGAAC GCTCGGACAG CGTCCTTCCA
    GCCTCTCACG GGCACCTCCC CCAGGCTGGC TCACTGGAGC GGAACCGCGT GGGAGTCTCC
    TCCAAACCGG ACAGCTCCCC TGTGCTCTCC CCTGGGAATA AAGCCAAGCC CGACGACCAC
    CGCTCACGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTGA AAGAGCGGAC TCTGGACGAG
    GCCCCTCGGC CTCCCAAGAA GGCCATGGAC TACTCGTCGT CCAGCGAGGA GGTGGAAAGC
    AGTGAGGACG ACGAGGAGGA AGGCGAAGGC GGGCCAGCAG AGGGGAGCAG AGATACCCCT
    GGGGGCCGCA GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001308165.1
    CDS526..4248
    Translation

    Target ORF information:

    RefSeq Version NM_001321236.2
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001321236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATACAA GCGGAAGCAG
    CTGGAGGAGC AGCGGCAGTC AGAACGTCTC CAGAGGCAGC TGCAGCAGGA GCATGCCTAC
    CTCAAGTCCC TGCAGCAGCA GCAACAGCAG CAGCAGCTTC AGAAACAGCA GCAGCAGCAG
    CTCCTGCCTG GGGACAGGAA GCCCCTGTAC CATTATGGTC GGGGCATGAA TCCCGCTGAC
    AAACCAGCCT GGGCCCGAGA GGTAGAAGAG AGAACAAGGA TGAACAAGCA GCAGAACTCT
    CCCTTGGCCA AGAGCAAGCC AGGCAGCACG GGGCCTGAGC CCCCCATCCC CCAGGCCTCC
    CCAGGGCCCC CAGGACCCCT TTCCCAGACT CCTCCTATGC AGAGGCCGGT GGAGCCCCAG
    GAGGGACCGC ACAAGAGCCT GGTGGCACAC CGGGTCCCAC TGAAGCCATA TGCAGCACCT
    GTACCCCGAT CCCAGTCCCT GCAGGACCAG CCCACCCGAA ACCTGGCTGC CTTCCCAGCC
    TCCCATGACC CCGACCCTGC CATCCCCGCA CCCACTGCCA CGCCCAGTGC CCGAGGAGCT
    GTCATCCGCC AGAATTCAGA CCCCACCTCT GAAGGACCTG GCCCCAGCCC GAATCCCCCA
    GCCTGGGTCC GCCCAGATAA CGAGGCCCCA CCCAAGGTGC CTCAGAGGAC CTCATCTATC
    GCCACTGCCC TTAACACCAG TGGGGCCGGA GGGTCCCGGC CAGCCCAGGC AGTCCGTGCC
    AGTAACCCCG ACCTCAGGAG GAGCGACCCT GGCTGGGAAC GCTCGGACAG CGTCCTTCCA
    GCCTCTCACG GGCACCTCCC CCAGGCTGGC TCACTGGAGC GGAACCGCGT GGGAGTCTCC
    TCCAAACCGG ACAGCTCCCC TGTGCTCTCC CCTGGGAATA AAGCCAAGCC CGACGACCAC
    CGCTCACGGC CAGGCCGGCC CGCAGACTTT GTGTTGCTGA AAGAGCGGAC TCTGGACGAG
    GCCCCTCGGC CTCCCAAGAA GGCCATGGAC TACTCGTCGT CCAGCGAGGA GGTGGAAAGC
    AGTGAGGACG ACGAGGAGGA AGGCGAAGGC GGGCCAGCAG AGGGGAGCAG AGATACCCCT
    GGGGGCCGCA GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49849
    Clone ID Related Accession (Same CDS sequence) XM_006721531.2
    Accession Version XM_006721531.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCAGCGATGG GGATACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTC
    GAGGAGATCA CCGGGACCCA GCCCCCATAC GGGGGCGGCA CCATGGTGGT CCAGCGCACC
    CCTGAAGAGG AGCGGAACCT GCTGCATGCT GACAGCAATG GGTACACAAA CCTGCCTGAC
    GTGGTCCAGC CCAGCCACTC ACCCACCGAG AACAGCAAAG GCCAAAGCCC ACCCTCGAAG
    GATGGGAGTG GTGACTACCA GTCTCGTGGG CTGGTAAAGG CCCCTGGCAA GAGCTCGTTC
    ACGATGTTTG TGGATCTAGG GATCTACCAG CCTGGAGGCA GTGGGGACAG CATCCCCATC
    ACAGCCCTAG TGGGTGGAGA GGGCACTCGG CTCGACCAGC TGCAGTACGA CGTGAGGAAG
    GGTTCTGTGG TCAACGTGAA TCCCACCAAC ACCCGGGCCC ACAGTGAGAC CCCTGAGATC
    CGGAAGTACA AGAAGCGATT CAACTCCGAG ATCCTCTGTG CAGCCCTTTG GGGGGTCAAC
    CTGCTGGTGG GCACGGAGAA CGGGCTGATG TTGCTGGACC GAAGTGGGCA GGGCAAGGTG
    TATGGACTCA TTGGGCGGCG ACGCTTCCAG CAGATGGATG TGCTGGAGGG GCTCAACCTG
    CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG
    AACAAGATTC TGCACAATGA CCCAGAAGTG GAGAAGAAGC AGGGCTGGAC CACCGTGGGG
    GACATGGAGG GCTGCGGGCA CTACCGTGTT GTGAAATACG AGCGGATTAA GTTCCTGGTC
    ATCGCCCTCA AGAGCTCCGT GGAGGTGTAT GCCTGGGCCC CCAAACCCTA CCACAAATTC
    ATGGCCTTCA AGTCCTTTGC CGACCTCCCC CACCGCCCTC TGCTGGTCGA CCTGACAGTA
    GAGGAGGGGC AGCGGCTCAA GGTCATCTAT GGCTCCAGTG CTGGCTTCCA TGCTGTGGAT
    GTCGACTCGG GGAACAGCTA TGACATCTAC ATCCCTGTGC ACATCCAGAG CCAGATCACG
    CCCCATGCCA TCATCTTCCT CCCCAACACC GACGGCATGG AGATGCTGCT GTGCTACGAG
    GACGAGGGTG TCTACGTCAA CACGTACGGG CGCATCATTA AGGATGTGGT GCTGCAGTGG
    GGGGAGATGC CTACTTCTGT GGCCTACATC TGCTCCAACC AGATAATGGG CTGGGGTGAG
    AAAGCCATTG AGATCCGCTC TGTGGAGACG GGCCACCTCG ACGGGGTCTT CATGCACAAA
    CGAGCTCAGA GGCTCAAGTT CCTGTGTGAG CGGAATGACA AGGTGTTTTT TGCCTCAGTC
    CGCTCTGGGG GCAGCAGCCA AGTTTACTTC ATGACTCTGA ACCGTAACTG CATCATGAAC
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721594.1
    CDS245..4330
    Translation

    Target ORF information:

    RefSeq Version XM_006721531.2
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721531.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCAGCGATGG GGATACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTC
    GAGGAGATCA CCGGGACCCA GCCCCCATAC GGGGGCGGCA CCATGGTGGT CCAGCGCACC
    CCTGAAGAGG AGCGGAACCT GCTGCATGCT GACAGCAATG GGTACACAAA CCTGCCTGAC
    GTGGTCCAGC CCAGCCACTC ACCCACCGAG AACAGCAAAG GCCAAAGCCC ACCCTCGAAG
    GATGGGAGTG GTGACTACCA GTCTCGTGGG CTGGTAAAGG CCCCTGGCAA GAGCTCGTTC
    ACGATGTTTG TGGATCTAGG GATCTACCAG CCTGGAGGCA GTGGGGACAG CATCCCCATC
    ACAGCCCTAG TGGGTGGAGA GGGCACTCGG CTCGACCAGC TGCAGTACGA CGTGAGGAAG
    GGTTCTGTGG TCAACGTGAA TCCCACCAAC ACCCGGGCCC ACAGTGAGAC CCCTGAGATC
    CGGAAGTACA AGAAGCGATT CAACTCCGAG ATCCTCTGTG CAGCCCTTTG GGGGGTCAAC
    CTGCTGGTGG GCACGGAGAA CGGGCTGATG TTGCTGGACC GAAGTGGGCA GGGCAAGGTG
    TATGGACTCA TTGGGCGGCG ACGCTTCCAG CAGATGGATG TGCTGGAGGG GCTCAACCTG
    CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG
    AACAAGATTC TGCACAATGA CCCAGAAGTG GAGAAGAAGC AGGGCTGGAC CACCGTGGGG
    GACATGGAGG GCTGCGGGCA CTACCGTGTT GTGAAATACG AGCGGATTAA GTTCCTGGTC
    ATCGCCCTCA AGAGCTCCGT GGAGGTGTAT GCCTGGGCCC CCAAACCCTA CCACAAATTC
    ATGGCCTTCA AGTCCTTTGC CGACCTCCCC CACCGCCCTC TGCTGGTCGA CCTGACAGTA
    GAGGAGGGGC AGCGGCTCAA GGTCATCTAT GGCTCCAGTG CTGGCTTCCA TGCTGTGGAT
    GTCGACTCGG GGAACAGCTA TGACATCTAC ATCCCTGTGC ACATCCAGAG CCAGATCACG
    CCCCATGCCA TCATCTTCCT CCCCAACACC GACGGCATGG AGATGCTGCT GTGCTACGAG
    GACGAGGGTG TCTACGTCAA CACGTACGGG CGCATCATTA AGGATGTGGT GCTGCAGTGG
    GGGGAGATGC CTACTTCTGT GGCCTACATC TGCTCCAACC AGATAATGGG CTGGGGTGAG
    AAAGCCATTG AGATCCGCTC TGTGGAGACG GGCCACCTCG ACGGGGTCTT CATGCACAAA
    CGAGCTCAGA GGCTCAAGTT CCTGTGTGAG CGGAATGACA AGGTGTTTTT TGCCTCAGTC
    CGCTCTGGGG GCAGCAGCCA AGTTTACTTC ATGACTCTGA ACCGTAACTG CATCATGAAC
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49850
    Clone ID Related Accession (Same CDS sequence) XM_006721532.2
    Accession Version XM_006721532.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721595.1
    CDS245..4327
    Translation

    Target ORF information:

    RefSeq Version XM_006721532.2
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721532.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCGATGGGGA TACAGACAGC GTCAGCACCA TGGTGGTCCA CGACGTCGAG
    GAGATCACCG GGACCCAGCC CCCATACGGG GGCGGCACCA TGGTGGTCCA GCGCACCCCT
    GAAGAGGAGC GGAACCTGCT GCATGCTGAC AGCAATGGGT ACACAAACCT GCCTGACGTG
    GTCCAGCCCA GCCACTCACC CACCGAGAAC AGCAAAGGCC AAAGCCCACC CTCGAAGGAT
    GGGAGTGGTG ACTACCAGTC TCGTGGGCTG GTAAAGGCCC CTGGCAAGAG CTCGTTCACG
    ATGTTTGTGG ATCTAGGGAT CTACCAGCCT GGAGGCAGTG GGGACAGCAT CCCCATCACA
    GCCCTAGTGG GTGGAGAGGG CACTCGGCTC GACCAGCTGC AGTACGACGT GAGGAAGGGT
    TCTGTGGTCA ACGTGAATCC CACCAACACC CGGGCCCACA GTGAGACCCC TGAGATCCGG
    AAGTACAAGA AGCGATTCAA CTCCGAGATC CTCTGTGCAG CCCTTTGGGG GGTCAACCTG
    CTGGTGGGCA CGGAGAACGG GCTGATGTTG CTGGACCGAA GTGGGCAGGG CAAGGTGTAT
    GGACTCATTG GGCGGCGACG CTTCCAGCAG ATGGATGTGC TGGAGGGGCT CAACCTGCTC
    ATCACCATCT CAGGGAAAAG GAACAAACTG CGGGTGTATT ACCTGTCCTG GCTCCGGAAC
    AAGATTCTGC ACAATGACCC AGAAGTGGAG AAGAAGCAGG GCTGGACCAC CGTGGGGGAC
    ATGGAGGGCT GCGGGCACTA CCGTGTTGTG AAATACGAGC GGATTAAGTT CCTGGTCATC
    GCCCTCAAGA GCTCCGTGGA GGTGTATGCC TGGGCCCCCA AACCCTACCA CAAATTCATG
    GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC CGCCCTCTGC TGGTCGACCT GACAGTAGAG
    GAGGGGCAGC GGCTCAAGGT CATCTATGGC TCCAGTGCTG GCTTCCATGC TGTGGATGTC
    GACTCGGGGA ACAGCTATGA CATCTACATC CCTGTGCACA TCCAGAGCCA GATCACGCCC
    CATGCCATCA TCTTCCTCCC CAACACCGAC GGCATGGAGA TGCTGCTGTG CTACGAGGAC
    GAGGGTGTCT ACGTCAACAC GTACGGGCGC ATCATTAAGG ATGTGGTGCT GCAGTGGGGG
    GAGATGCCTA CTTCTGTGGC CTACATCTGC TCCAACCAGA TAATGGGCTG GGGTGAGAAA
    GCCATTGAGA TCCGCTCTGT GGAGACGGGC CACCTCGACG GGGTCTTCAT GCACAAACGA
    GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG AATGACAAGG TGTTTTTTGC CTCAGTCCGC
    TCTGGGGGCA GCAGCCAAGT TTACTTCATG ACTCTGAACC GTAACTGCAT CATGAACTGG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu49855
    Clone ID Related Accession (Same CDS sequence) XM_006721536.2
    Accession Version XM_006721536.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006721536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGTAACCC CGACCTCAGG AGGAGCGACC CTGGCTGGGA ACGCTCGGAC
    AGCGTCCTTC CAGCCTCTCA CGGGCACCTC CCCCAGGCTG GCTCACTGGA GCGGAACCGC
    GTGGGAGTCT CCTCCAAACC GGACAGCTCC CCTGTGCTCT CCCCTGGGAA TAAAGCCAAG
    CCCGACGACC ACCGCTCACG GCCAGGCCGG CCCGCAGACT TTGTGTTGCT GAAAGAGCGG
    ACTCTGGACG AGGCCCCTCG GCCTCCCAAG AAGGCCATGG ACTACTCGTC GTCCAGCGAG
    GAGGTGGAAA GCAGTGAGGA CGACGAGGAG GAAGGCGAAG GCGGGCCAGC AGAGGGGAGC
    AGAGATACCC CTGGGGGCCG CAGCGATGGG GATACAGACA GCGTCAGCAC CATGGTGGTC
    CACGACGTCG AGGAGATCAC CGGGACCCAG CCCCCATACG GGGGCGGCAC CATGGTGGTC
    CAGCGCACCC CTGAAGAGGA GCGGAACCTG CTGCATGCTG ACAGCAATGG GTACACAAAC
    CTGCCTGACG TGGTCCAGCC CAGCCACTCA CCCACCGAGA ACAGCAAAGG CCAAAGCCCA
    CCCTCGAAGG ATGGGAGTGG TGACTACCAG TCTCGTGGGC TGGTAAAGGC CCCTGGCAAG
    AGCTCGTTCA CGATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGACAGC
    ATCCCCATCA CAGCCCTAGT GGGTGGAGAG GGCACTCGGC TCGACCAGCT GCAGTACGAC
    GTGAGGAAGG GTTCTGTGGT CAACGTGAAT CCCACCAACA CCCGGGCCCA CAGTGAGACC
    CCTGAGATCC GGAAGTACAA GAAGCGATTC AACTCCGAGA TCCTCTGTGC AGCCCTTTGG
    GGGGTCAACC TGCTGGTGGG CACGGAGAAC GGGCTGATGT TGCTGGACCG AAGTGGGCAG
    GGCAAGGTGT ATGGACTCAT TGGGCGGCGA CGCTTCCAGC AGATGGATGT GCTGGAGGGG
    CTCAACCTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTGTA TTACCTGTCC
    TGGCTCCGGA ACAAGATTCT GCACAATGAC CCAGAAGTGG AGAAGAAGCA GGGCTGGACC
    ACCGTGGGGG ACATGGAGGG CTGCGGGCAC TACCGTGTTG TGAAATACGA GCGGATTAAG
    TTCCTGGTCA TCGCCCTCAA GAGCTCCGTG GAGGTGTATG CCTGGGCCCC CAAACCCTAC
    CACAAATTCA TGGCCTTCAA GTCCTTTGCC GACCTCCCCC ACCGCCCTCT GCTGGTCGAC
    CTGACAGTAG AGGAGGGGCA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT
    GCTGTGGATG TCGACTCGGG GAACAGCTAT GACATCTACA TCCCTGTGCA CATCCAGAGC
    CAGATCACGC CCCATGCCAT CATCTTCCTC CCCAACACCG ACGGCATGGA GATGCTGCTG
    TGCTACGAGG ACGAGGGTGT CTACGTCAAC ACGTACGGGC GCATCATTAA GGATGTGGTG
    CTGCAGTGGG GGGAGATGCC TACTTCTGTG GCCTACATCT GCTCCAACCA GATAATGGGC
    TGGGGTGAGA AAGCCATTGA GATCCGCTCT GTGGAGACGG GCCACCTCGA CGGGGTCTTC
    ATGCACAAAC GAGCTCAGAG GCTCAAGTTC CTGTGTGAGC GGAATGACAA GGTGTTTTTT
    GCCTCAGTCC GCTCTGGGGG CAGCAGCCAA GTTTACTTCA TGACTCTGAA CCGTAACTGC
    ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006721599.1
    CDS245..4219
    Translation

    Target ORF information:

    RefSeq Version XM_006721536.2
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006721536.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGTAACCC CGACCTCAGG AGGAGCGACC CTGGCTGGGA ACGCTCGGAC
    AGCGTCCTTC CAGCCTCTCA CGGGCACCTC CCCCAGGCTG GCTCACTGGA GCGGAACCGC
    GTGGGAGTCT CCTCCAAACC GGACAGCTCC CCTGTGCTCT CCCCTGGGAA TAAAGCCAAG
    CCCGACGACC ACCGCTCACG GCCAGGCCGG CCCGCAGACT TTGTGTTGCT GAAAGAGCGG
    ACTCTGGACG AGGCCCCTCG GCCTCCCAAG AAGGCCATGG ACTACTCGTC GTCCAGCGAG
    GAGGTGGAAA GCAGTGAGGA CGACGAGGAG GAAGGCGAAG GCGGGCCAGC AGAGGGGAGC
    AGAGATACCC CTGGGGGCCG CAGCGATGGG GATACAGACA GCGTCAGCAC CATGGTGGTC
    CACGACGTCG AGGAGATCAC CGGGACCCAG CCCCCATACG GGGGCGGCAC CATGGTGGTC
    CAGCGCACCC CTGAAGAGGA GCGGAACCTG CTGCATGCTG ACAGCAATGG GTACACAAAC
    CTGCCTGACG TGGTCCAGCC CAGCCACTCA CCCACCGAGA ACAGCAAAGG CCAAAGCCCA
    CCCTCGAAGG ATGGGAGTGG TGACTACCAG TCTCGTGGGC TGGTAAAGGC CCCTGGCAAG
    AGCTCGTTCA CGATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGACAGC
    ATCCCCATCA CAGCCCTAGT GGGTGGAGAG GGCACTCGGC TCGACCAGCT GCAGTACGAC
    GTGAGGAAGG GTTCTGTGGT CAACGTGAAT CCCACCAACA CCCGGGCCCA CAGTGAGACC
    CCTGAGATCC GGAAGTACAA GAAGCGATTC AACTCCGAGA TCCTCTGTGC AGCCCTTTGG
    GGGGTCAACC TGCTGGTGGG CACGGAGAAC GGGCTGATGT TGCTGGACCG AAGTGGGCAG
    GGCAAGGTGT ATGGACTCAT TGGGCGGCGA CGCTTCCAGC AGATGGATGT GCTGGAGGGG
    CTCAACCTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTGTA TTACCTGTCC
    TGGCTCCGGA ACAAGATTCT GCACAATGAC CCAGAAGTGG AGAAGAAGCA GGGCTGGACC
    ACCGTGGGGG ACATGGAGGG CTGCGGGCAC TACCGTGTTG TGAAATACGA GCGGATTAAG
    TTCCTGGTCA TCGCCCTCAA GAGCTCCGTG GAGGTGTATG CCTGGGCCCC CAAACCCTAC
    CACAAATTCA TGGCCTTCAA GTCCTTTGCC GACCTCCCCC ACCGCCCTCT GCTGGTCGAC
    CTGACAGTAG AGGAGGGGCA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT
    GCTGTGGATG TCGACTCGGG GAACAGCTAT GACATCTACA TCCCTGTGCA CATCCAGAGC
    CAGATCACGC CCCATGCCAT CATCTTCCTC CCCAACACCG ACGGCATGGA GATGCTGCTG
    TGCTACGAGG ACGAGGGTGT CTACGTCAAC ACGTACGGGC GCATCATTAA GGATGTGGTG
    CTGCAGTGGG GGGAGATGCC TACTTCTGTG GCCTACATCT GCTCCAACCA GATAATGGGC
    TGGGGTGAGA AAGCCATTGA GATCCGCTCT GTGGAGACGG GCCACCTCGA CGGGGTCTTC
    ATGCACAAAC GAGCTCAGAG GCTCAAGTTC CTGTGTGAGC GGAATGACAA GGTGTTTTTT
    GCCTCAGTCC GCTCTGGGGG CAGCAGCCAA GTTTACTTCA TGACTCTGAA CCGTAACTGC
    ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67500
    Clone ID Related Accession (Same CDS sequence) XM_011523906.1
    Accession Version XM_011523906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG AGGAGACAGA ATATGAGTAC
    AGCGGCAGCG AGGAGGAAGA TGACAGCCAT GGAGAGGAAG GAGAGCCAAG CTCCATCATG
    AACGTGCCTG GAGAGTCGAC TCTACGCCGG GAGTTTCTCC GGCTCCAGCA GGAAAATAAG
    AGCAACTCAG AGGCTTTAAA ACAGCAGCAG CAGCTGCAGC AGCAGCAGCA GCGAGACCCC
    GAGGCACACA TCAAACACCT GCTGCACCAG CGGCAGCGGC GCATAGAGGA GCAGAAGGAG
    GAGCGGCGCC GCGTGGAGGA GCAACAGCGG CGGGAGCGGG AGCAGCGGAA GCTGCAGGAG
    AAGGAGCAGC AGCGGCGGCT GGAGGACATG CAGGCTCTGC GGCGGGAGGA GGAGCGGCGG
    CAGGCGGAGC GCGAGCAGGA ATACAAGCGG AAGCAGCTGG AGGAGCAGCG GCAGTCAGAA
    CGTCTCCAGA GGCAGCTGCA GCAGGAGCAT GCCTACCTCA AGTCCCTGCA GCAGCAGCAA
    CAGCAGCAGC AGCTTCAGAA ACAGCAGCAG CAGCAGCTCC TGCCTGGGGA CAGGAAGCCC
    CTGTACCATT ATGGTCGGGG CATGAATCCC GCTGACAAAC CAGCCTGGGC CCGAGAGGTA
    GAAGAGAGAA CAAGGATGAA CAAGCAGCAG AACTCTCCCT TGGCCAAGAG CAAGCCAGGC
    AGCACGGGGC CTGAGCCCCC CATCCCCCAG GCCTCCCCAG GGCCCCCAGG ACCCCTTTCC
    CAGACTCCTC CTATGCAGAG GCCGGTGGAG CCCCAGGAGG GACCGCACAA GAGCCTGGTG
    GCACACCGGG TCCCACTGAA GCCATATGCA GCACCTGTAC CCCGATCCCA GTCCCTGCAG
    GACCAGCCCA CCCGAAACCT GGCTGCCTTC CCAGCCTCCC ATGACCCCGA CCCTGCCATC
    CCCGCACCCA CTGCCACGCC CAGTGCCCGA GGAGCTGTCA TCCGCCAGAA TTCAGACCCC
    ACCTCTGAAG GACCTGGCCC CAGCCCGAAT CCCCCAGCCT GGGTCCGCCC AGATAACGAG
    GCCCCACCCA AGGTGCCTCA GAGGACCTCA TCTATCGCCA CTGCCCTTAA CACCAGTGGG
    GCCGGAGGGT CCCGGCCAGC CCAGGCAGTC CGTGCCAGAC CTCGCAGCAA CTCCGCCTGG
    CAAATCTATC TGCAAAGGCG GGCAGAGCGG GGCACCCCAA AGCCTCCAGG GCCCCCTGCT
    CAGCCCCCTG GCCCGCCCAA CGCCTCTAGT AACCCCGACC TCAGGAGGAG CGACCCTGGC
    TGGGAACGCT CGGACAGCGT CCTTCCAGCC TCTCACGGGC ACCTCCCCCA GGCTGGCTCA
    CTGGAGCGGA ACCGCGTGGG AGTCTCCTCC AAACCGGACA GCTCCCCTGT GCTCTCCCCT
    GGGAATAAAG CCAAGCCCGA CGACCACCGC TCACGGCCAG GCCGGCCCGC AGACTTTGTG
    TTGCTGAAAG AGCGGACTCT GGACGAGGCC CCTCGGCCTC CCAAGAAGGC CATGGACTAC
    TCGTCGTCCA GCGAGGAGGT GGAAAGCAGT GAGGACGACG AGGAGGAAGG CGAAGGCGGG
    CCAGCAGAGG GGAGCAGAGA TACCCCTGGG GGCCGCAGCG ATGGGGATAC AGACAGCGTC
    AGCACCATGG TGGTCCACGA CGTCGAGGAG ATCACCGGGA CCCAGCCCCC ATACGGGGGC
    GGCACCATGG TGGTCCAGCG CACCCCTGAA GAGGAGCGGA ACCTGCTGCA TGCTGACAGC
    AATGGGTACA CAAACCTGCC TGACGTGGTC CAGCCCAGCC ACTCACCCAC CGAGAACAGC
    AAAGGCCAAA GCCCACCCTC GAAGGATGGG AGTGGTGACT ACCAGTCTCG TGGGCTGGTA
    AAGGCCCCTG GCAAGAGCTC GTTCACGATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA
    GGCAGTGGGG ACAGCATCCC CATCACAGCC CTAGTGGGTG GAGAGGGCAC TCGGCTCGAC
    CAGCTGCAGT ACGACGTGAG GAAGGGTTCT GTGGTCAACG TGAATCCCAC CAACACCCGG
    GCCCACAGTG AGACCCCTGA GATCCGGAAG TACAAGAAGC GATTCAACTC CGAGATCCTC
    TGTGCAGCCC TTTGGGGGGT CAACCTGCTG GTGGGCACGG AGAACGGGCT GATGTTGCTG
    GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTCATTGGGC GGCGACGCTT CCAGCAGATG
    GATGTGCTGG AGGGGCTCAA CCTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG
    GTGTATTACC TGTCCTGGCT CCGGAACAAG ATTCTGCACA ATGACCCAGA AGTGGAGAAG
    AAGCAGGGCT GGACCACCGT GGGGGACATG GAGGGCTGCG GGCACTACCG TGTTGTGAAA
    TACGAGCGGA TTAAGTTCCT GGTCATCGCC CTCAAGAGCT CCGTGGAGGT GTATGCCTGG
    GCCCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCCGACCT CCCCCACCGC
    CCTCTGCTGG TCGACCTGAC AGTAGAGGAG GGGCAGCGGC TCAAGGTCAT CTATGGCTCC
    AGTGCTGGCT TCCATGCTGT GGATGTCGAC TCGGGGAACA GCTATGACAT CTACATCCCT
    GTGCACATCC AGAGCCAGAT CACGCCCCAT GCCATCATCT TCCTCCCCAA CACCGACGGC
    ATGGAGATGC TGCTGTGCTA CGAGGACGAG GGTGTCTACG TCAACACGTA CGGGCGCATC
    ATTAAGGATG TGGTGCTGCA GTGGGGGGAG ATGCCTACTT CTGTGGCCTA CATCTGCTCC
    AACCAGATAA TGGGCTGGGG TGAGAAAGCC ATTGAGATCC GCTCTGTGGA GACGGGCCAC
    CTCGACGGGG TCTTCATGCA CAAACGAGCT CAGAGGCTCA AGTTCCTGTG TGAGCGGAAT
    GACAAGGTGT TTTTTGCCTC AGTCCGCTCT GGGGGCAGCA GCCAAGTTTA CTTCATGACT
    CTGAACCGTA ACTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011522208.1
    CDS245..4234
    Translation

    Target ORF information:

    RefSeq Version XM_011523906.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011523906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG AGGAGACAGA ATATGAGTAC
    AGCGGCAGCG AGGAGGAAGA TGACAGCCAT GGAGAGGAAG GAGAGCCAAG CTCCATCATG
    AACGTGCCTG GAGAGTCGAC TCTACGCCGG GAGTTTCTCC GGCTCCAGCA GGAAAATAAG
    AGCAACTCAG AGGCTTTAAA ACAGCAGCAG CAGCTGCAGC AGCAGCAGCA GCGAGACCCC
    GAGGCACACA TCAAACACCT GCTGCACCAG CGGCAGCGGC GCATAGAGGA GCAGAAGGAG
    GAGCGGCGCC GCGTGGAGGA GCAACAGCGG CGGGAGCGGG AGCAGCGGAA GCTGCAGGAG
    AAGGAGCAGC AGCGGCGGCT GGAGGACATG CAGGCTCTGC GGCGGGAGGA GGAGCGGCGG
    CAGGCGGAGC GCGAGCAGGA ATACAAGCGG AAGCAGCTGG AGGAGCAGCG GCAGTCAGAA
    CGTCTCCAGA GGCAGCTGCA GCAGGAGCAT GCCTACCTCA AGTCCCTGCA GCAGCAGCAA
    CAGCAGCAGC AGCTTCAGAA ACAGCAGCAG CAGCAGCTCC TGCCTGGGGA CAGGAAGCCC
    CTGTACCATT ATGGTCGGGG CATGAATCCC GCTGACAAAC CAGCCTGGGC CCGAGAGGTA
    GAAGAGAGAA CAAGGATGAA CAAGCAGCAG AACTCTCCCT TGGCCAAGAG CAAGCCAGGC
    AGCACGGGGC CTGAGCCCCC CATCCCCCAG GCCTCCCCAG GGCCCCCAGG ACCCCTTTCC
    CAGACTCCTC CTATGCAGAG GCCGGTGGAG CCCCAGGAGG GACCGCACAA GAGCCTGGTG
    GCACACCGGG TCCCACTGAA GCCATATGCA GCACCTGTAC CCCGATCCCA GTCCCTGCAG
    GACCAGCCCA CCCGAAACCT GGCTGCCTTC CCAGCCTCCC ATGACCCCGA CCCTGCCATC
    CCCGCACCCA CTGCCACGCC CAGTGCCCGA GGAGCTGTCA TCCGCCAGAA TTCAGACCCC
    ACCTCTGAAG GACCTGGCCC CAGCCCGAAT CCCCCAGCCT GGGTCCGCCC AGATAACGAG
    GCCCCACCCA AGGTGCCTCA GAGGACCTCA TCTATCGCCA CTGCCCTTAA CACCAGTGGG
    GCCGGAGGGT CCCGGCCAGC CCAGGCAGTC CGTGCCAGAC CTCGCAGCAA CTCCGCCTGG
    CAAATCTATC TGCAAAGGCG GGCAGAGCGG GGCACCCCAA AGCCTCCAGG GCCCCCTGCT
    CAGCCCCCTG GCCCGCCCAA CGCCTCTAGT AACCCCGACC TCAGGAGGAG CGACCCTGGC
    TGGGAACGCT CGGACAGCGT CCTTCCAGCC TCTCACGGGC ACCTCCCCCA GGCTGGCTCA
    CTGGAGCGGA ACCGCGTGGG AGTCTCCTCC AAACCGGACA GCTCCCCTGT GCTCTCCCCT
    GGGAATAAAG CCAAGCCCGA CGACCACCGC TCACGGCCAG GCCGGCCCGC AGACTTTGTG
    TTGCTGAAAG AGCGGACTCT GGACGAGGCC CCTCGGCCTC CCAAGAAGGC CATGGACTAC
    TCGTCGTCCA GCGAGGAGGT GGAAAGCAGT GAGGACGACG AGGAGGAAGG CGAAGGCGGG
    CCAGCAGAGG GGAGCAGAGA TACCCCTGGG GGCCGCAGCG ATGGGGATAC AGACAGCGTC
    AGCACCATGG TGGTCCACGA CGTCGAGGAG ATCACCGGGA CCCAGCCCCC ATACGGGGGC
    GGCACCATGG TGGTCCAGCG CACCCCTGAA GAGGAGCGGA ACCTGCTGCA TGCTGACAGC
    AATGGGTACA CAAACCTGCC TGACGTGGTC CAGCCCAGCC ACTCACCCAC CGAGAACAGC
    AAAGGCCAAA GCCCACCCTC GAAGGATGGG AGTGGTGACT ACCAGTCTCG TGGGCTGGTA
    AAGGCCCCTG GCAAGAGCTC GTTCACGATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA
    GGCAGTGGGG ACAGCATCCC CATCACAGCC CTAGTGGGTG GAGAGGGCAC TCGGCTCGAC
    CAGCTGCAGT ACGACGTGAG GAAGGGTTCT GTGGTCAACG TGAATCCCAC CAACACCCGG
    GCCCACAGTG AGACCCCTGA GATCCGGAAG TACAAGAAGC GATTCAACTC CGAGATCCTC
    TGTGCAGCCC TTTGGGGGGT CAACCTGCTG GTGGGCACGG AGAACGGGCT GATGTTGCTG
    GACCGAAGTG GGCAGGGCAA GGTGTATGGA CTCATTGGGC GGCGACGCTT CCAGCAGATG
    GATGTGCTGG AGGGGCTCAA CCTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG
    GTGTATTACC TGTCCTGGCT CCGGAACAAG ATTCTGCACA ATGACCCAGA AGTGGAGAAG
    AAGCAGGGCT GGACCACCGT GGGGGACATG GAGGGCTGCG GGCACTACCG TGTTGTGAAA
    TACGAGCGGA TTAAGTTCCT GGTCATCGCC CTCAAGAGCT CCGTGGAGGT GTATGCCTGG
    GCCCCCAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCCGACCT CCCCCACCGC
    CCTCTGCTGG TCGACCTGAC AGTAGAGGAG GGGCAGCGGC TCAAGGTCAT CTATGGCTCC
    AGTGCTGGCT TCCATGCTGT GGATGTCGAC TCGGGGAACA GCTATGACAT CTACATCCCT
    GTGCACATCC AGAGCCAGAT CACGCCCCAT GCCATCATCT TCCTCCCCAA CACCGACGGC
    ATGGAGATGC TGCTGTGCTA CGAGGACGAG GGTGTCTACG TCAACACGTA CGGGCGCATC
    ATTAAGGATG TGGTGCTGCA GTGGGGGGAG ATGCCTACTT CTGTGGCCTA CATCTGCTCC
    AACCAGATAA TGGGCTGGGG TGAGAAAGCC ATTGAGATCC GCTCTGTGGA GACGGGCCAC
    CTCGACGGGG TCTTCATGCA CAAACGAGCT CAGAGGCTCA AGTTCCTGTG TGAGCGGAAT
    GACAAGGTGT TTTTTGCCTC AGTCCGCTCT GGGGGCAGCA GCCAAGTTTA CTTCATGACT
    CTGAACCGTA ACTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86902
    Clone ID Related Accession (Same CDS sequence) XM_017024704.1
    Accession Version XM_017024704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4077bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG AGGAGACAGA ATATGAGTAC
    AGCGGCAGCG AGGAGGAAGA TGACAGCCAT GGAGAGGAAG GAGAGCCAAG CTCCATCATG
    AACGTGCCTG GAGAGTCGAC TCTACGCCGG GAGTTTCTCC GGCTCCAGCA GGAAAATAAG
    AGCAACTCAG AGGCTTTAAA ACAGCAGCAG CAGCTGCAGC AGCAGCAGCA GCGAGACCCC
    GAGGCACACA TCAAACACCT GCTGCACCAG CGGCAGCGGC GCATAGAGGA GCAGAAGGAG
    GAGCGGCGCC GCGTGGAGGA GCAACAGCGG CGGGAGCGGG AGCAGCGGAA GCTGCAGGAG
    AAGGAGCAGC AGCGGCGGCT GGAGGACATG CAGGCTCTGC GGCGGGAGGA GGAGCGGCGG
    CAGGCGGAGC GCGAGCAGGA ATATATTCGT CACAGGCTAG AGGAGGAGCA GCGACAGCTC
    GAGATCCTTC AGCAACAGCT GCTCCAGGAA CAGGCCCTGC TGCTGGAATA CAAGCGGAAG
    CAGCTGGAGG AGCAGCGGCA GTCAGAACGT CTCCAGAGGC AGCTGCAGCA GGAGCATGCC
    TACCTCAAGT CCCTGCAGCA GCAGCAACAG CAGCAGCAGC TTCAGAAACA GCAGCAGCAG
    CAGCTCCTGC CTGGGGACAG GAAGCCCCTG TACCATTATG GTCGGGGCAT GAATCCCGCT
    GACAAACCAG CCTGGGCCCG AGAGGTAGAA GAGAGAACAA GGATGAACAA GCAGCAGAAC
    TCTCCCTTGG CCAAGAGCAA GCCAGGCAGC ACGGGGCCTG AGCCCCCCAT CCCCCAGGCC
    TCCCCAGGGC CCCCAGGACC CCTTTCCCAG ACTCCTCCTA TGCAGAGGCC GGTGGAGCCC
    CAGGAGGGAC CGCACAAGAG CCTGGTGGCA CACCGGGTCC CACTGAAGCC ATATGCAGCA
    CCTGTACCCC GATCCCAGTC CCTGCAGGAC CAGCCCACCC GAAACCTGGC TGCCTTCCCA
    GCCTCCCATG ACCCCGACCC TGCCATCCCC GCACCCACTG CCACGCCCAG TGCCCGAGGA
    GCTGTCATCC GCCAGAATTC AGACCCCACC TCTGAAGGAC CTGGCCCCAG CCCGAATCCC
    CCAGCCTGGG TCCGCCCAGA TAACGAGGCC CCACCCAAGG TGCCTCAGAG GACCTCATCT
    ATCGCCACTG CCCTTAACAC CAGTGGGGCC GGAGGGTCCC GGCCAGCCCA GGCAGTCCGT
    GCCAGACCTC GCAGCAACTC CGCCTGGCAA ATCTATCTGC AAAGGCGGGC AGAGCGGGGC
    ACCCCAAAGC CTCCAGGGCC CCCTGCTCAG CCCCCTGGCC CGCCCAACGC CTCTAGTAAC
    CCCGACCTCA GGAGGAGCGA CCCTGGCTGG GAACGCTCGG ACAGCGTCCT TCCAGCCTCT
    CACGGGCACC TCCCCCAGGC TGGCTCACTG GAGCGGAACC GCGTGGGAGT CTCCTCCAAA
    CCGGACAGCT CCCCTGTGCT CTCCCCTGGG AATAAAGCCA AGCCCGACGA CCACCGCTCA
    CGGCCAGGCC GGCCCGCAGA CTTTGTGTTG CTGAAAGAGC GGACTCTGGA CGAGGCCCCT
    CGGCCTCCCA AGAAGGCCAT GGACTACTCG TCGTCCAGCG AGGAGGTGGA AAGCAGTGAG
    GACGACGAGG AGGAAGGCGA AGGCGGGCCA GCAGAGGGGA GCAGAGATAC CCCTGGGGGC
    CGCAGCGATG GGGATACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT CGAGGAGATC
    ACCGGGACCC AGCCCCCATA CGGGGGCGGC ACCATGGTGG TCCAGCGCAC CCCTGAAGAG
    GAGCGGAACC TGCTGCATGC TGACAGCAAT GGGTACACAA ACCTGCCTGA CGTGGTCCAG
    CCCAGCCACT CACCCACCGA GAACAGCAAA GGCCAAAGCC CACCCTCGAA GGATGGGAGT
    GGTGACTACC AGTCTCGTGG GCTGGTAAAG GCCCCTGGCA AGAGCTCGTT CACGATGTTT
    GTGGATCTAG GGATCTACCA GCCTGGAGGC AGTGGGGACA GCATCCCCAT CACAGCCCTA
    GTGGGTGGAG AGGGCACTCG GCTCGACCAG CTGCAGTACG ACGTGAGGAA GGGTTCTGTG
    GTCAACGTGA ATCCCACCAA CACCCGGGCC CACAGTGAGA CCCCTGAGAT CCGGAAGTAC
    AAGAAGCGAT TCAACTCCGA GATCCTCTGT GCAGCCCTTT GGGGGGTCAA CCTGCTGGTG
    GGCACGGAGA ACGGGCTGAT GTTGCTGGAC CGAAGTGGGC AGGGCAAGGT GTATGGACTC
    ATTGGGCGGC GACGCTTCCA GCAGATGGAT GTGCTGGAGG GGCTCAACCT GCTCATCACC
    ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT
    CTGCACAATG ACCCAGAAGT GGAGAAGAAG CAGGGCTGGA CCACCGTGGG GGACATGGAG
    GGCTGCGGGC ACTACCGTGT TGTGAAATAC GAGCGGATTA AGTTCCTGGT CATCGCCCTC
    AAGAGCTCCG TGGAGGTGTA TGCCTGGGCC CCCAAACCCT ACCACAAATT CATGGCCTTC
    AAGTCCTTTG CCGACCTCCC CCACCGCCCT CTGCTGGTCG ACCTGACAGT AGAGGAGGGG
    CAGCGGCTCA AGGTCATCTA TGGCTCCAGT GCTGGCTTCC ATGCTGTGGA TGTCGACTCG
    GGGAACAGCT ATGACATCTA CATCCCTGTG CACATCCAGA GCCAGATCAC GCCCCATGCC
    ATCATCTTCC TCCCCAACAC CGACGGCATG GAGATGCTGC TGTGCTACGA GGACGAGGGT
    GTCTACGTCA ACACGTACGG GCGCATCATT AAGGATGTGG TGCTGCAGTG GGGGGAGATG
    CCTACTTCTG TGGCCTACAT CTGCTCCAAC CAGATAATGG GCTGGGGTGA GAAAGCCATT
    GAGATCCGCT CTGTGGAGAC GGGCCACCTC GACGGGGTCT TCATGCACAA ACGAGCTCAG
    AGGCTCAAGT TCCTGTGTGA GCGGAATGAC AAGGTGTTTT TTGCCTCAGT CCGCTCTGGG
    GGCAGCAGCC AAGTTTACTT CATGACTCTG AACCGTAACT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880193.1
    CDS245..4321
    Translation

    Target ORF information:

    RefSeq Version XM_017024704.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG AGGAGACAGA ATATGAGTAC
    AGCGGCAGCG AGGAGGAAGA TGACAGCCAT GGAGAGGAAG GAGAGCCAAG CTCCATCATG
    AACGTGCCTG GAGAGTCGAC TCTACGCCGG GAGTTTCTCC GGCTCCAGCA GGAAAATAAG
    AGCAACTCAG AGGCTTTAAA ACAGCAGCAG CAGCTGCAGC AGCAGCAGCA GCGAGACCCC
    GAGGCACACA TCAAACACCT GCTGCACCAG CGGCAGCGGC GCATAGAGGA GCAGAAGGAG
    GAGCGGCGCC GCGTGGAGGA GCAACAGCGG CGGGAGCGGG AGCAGCGGAA GCTGCAGGAG
    AAGGAGCAGC AGCGGCGGCT GGAGGACATG CAGGCTCTGC GGCGGGAGGA GGAGCGGCGG
    CAGGCGGAGC GCGAGCAGGA ATATATTCGT CACAGGCTAG AGGAGGAGCA GCGACAGCTC
    GAGATCCTTC AGCAACAGCT GCTCCAGGAA CAGGCCCTGC TGCTGGAATA CAAGCGGAAG
    CAGCTGGAGG AGCAGCGGCA GTCAGAACGT CTCCAGAGGC AGCTGCAGCA GGAGCATGCC
    TACCTCAAGT CCCTGCAGCA GCAGCAACAG CAGCAGCAGC TTCAGAAACA GCAGCAGCAG
    CAGCTCCTGC CTGGGGACAG GAAGCCCCTG TACCATTATG GTCGGGGCAT GAATCCCGCT
    GACAAACCAG CCTGGGCCCG AGAGGTAGAA GAGAGAACAA GGATGAACAA GCAGCAGAAC
    TCTCCCTTGG CCAAGAGCAA GCCAGGCAGC ACGGGGCCTG AGCCCCCCAT CCCCCAGGCC
    TCCCCAGGGC CCCCAGGACC CCTTTCCCAG ACTCCTCCTA TGCAGAGGCC GGTGGAGCCC
    CAGGAGGGAC CGCACAAGAG CCTGGTGGCA CACCGGGTCC CACTGAAGCC ATATGCAGCA
    CCTGTACCCC GATCCCAGTC CCTGCAGGAC CAGCCCACCC GAAACCTGGC TGCCTTCCCA
    GCCTCCCATG ACCCCGACCC TGCCATCCCC GCACCCACTG CCACGCCCAG TGCCCGAGGA
    GCTGTCATCC GCCAGAATTC AGACCCCACC TCTGAAGGAC CTGGCCCCAG CCCGAATCCC
    CCAGCCTGGG TCCGCCCAGA TAACGAGGCC CCACCCAAGG TGCCTCAGAG GACCTCATCT
    ATCGCCACTG CCCTTAACAC CAGTGGGGCC GGAGGGTCCC GGCCAGCCCA GGCAGTCCGT
    GCCAGACCTC GCAGCAACTC CGCCTGGCAA ATCTATCTGC AAAGGCGGGC AGAGCGGGGC
    ACCCCAAAGC CTCCAGGGCC CCCTGCTCAG CCCCCTGGCC CGCCCAACGC CTCTAGTAAC
    CCCGACCTCA GGAGGAGCGA CCCTGGCTGG GAACGCTCGG ACAGCGTCCT TCCAGCCTCT
    CACGGGCACC TCCCCCAGGC TGGCTCACTG GAGCGGAACC GCGTGGGAGT CTCCTCCAAA
    CCGGACAGCT CCCCTGTGCT CTCCCCTGGG AATAAAGCCA AGCCCGACGA CCACCGCTCA
    CGGCCAGGCC GGCCCGCAGA CTTTGTGTTG CTGAAAGAGC GGACTCTGGA CGAGGCCCCT
    CGGCCTCCCA AGAAGGCCAT GGACTACTCG TCGTCCAGCG AGGAGGTGGA AAGCAGTGAG
    GACGACGAGG AGGAAGGCGA AGGCGGGCCA GCAGAGGGGA GCAGAGATAC CCCTGGGGGC
    CGCAGCGATG GGGATACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT CGAGGAGATC
    ACCGGGACCC AGCCCCCATA CGGGGGCGGC ACCATGGTGG TCCAGCGCAC CCCTGAAGAG
    GAGCGGAACC TGCTGCATGC TGACAGCAAT GGGTACACAA ACCTGCCTGA CGTGGTCCAG
    CCCAGCCACT CACCCACCGA GAACAGCAAA GGCCAAAGCC CACCCTCGAA GGATGGGAGT
    GGTGACTACC AGTCTCGTGG GCTGGTAAAG GCCCCTGGCA AGAGCTCGTT CACGATGTTT
    GTGGATCTAG GGATCTACCA GCCTGGAGGC AGTGGGGACA GCATCCCCAT CACAGCCCTA
    GTGGGTGGAG AGGGCACTCG GCTCGACCAG CTGCAGTACG ACGTGAGGAA GGGTTCTGTG
    GTCAACGTGA ATCCCACCAA CACCCGGGCC CACAGTGAGA CCCCTGAGAT CCGGAAGTAC
    AAGAAGCGAT TCAACTCCGA GATCCTCTGT GCAGCCCTTT GGGGGGTCAA CCTGCTGGTG
    GGCACGGAGA ACGGGCTGAT GTTGCTGGAC CGAAGTGGGC AGGGCAAGGT GTATGGACTC
    ATTGGGCGGC GACGCTTCCA GCAGATGGAT GTGCTGGAGG GGCTCAACCT GCTCATCACC
    ATCTCAGGGA AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT
    CTGCACAATG ACCCAGAAGT GGAGAAGAAG CAGGGCTGGA CCACCGTGGG GGACATGGAG
    GGCTGCGGGC ACTACCGTGT TGTGAAATAC GAGCGGATTA AGTTCCTGGT CATCGCCCTC
    AAGAGCTCCG TGGAGGTGTA TGCCTGGGCC CCCAAACCCT ACCACAAATT CATGGCCTTC
    AAGTCCTTTG CCGACCTCCC CCACCGCCCT CTGCTGGTCG ACCTGACAGT AGAGGAGGGG
    CAGCGGCTCA AGGTCATCTA TGGCTCCAGT GCTGGCTTCC ATGCTGTGGA TGTCGACTCG
    GGGAACAGCT ATGACATCTA CATCCCTGTG CACATCCAGA GCCAGATCAC GCCCCATGCC
    ATCATCTTCC TCCCCAACAC CGACGGCATG GAGATGCTGC TGTGCTACGA GGACGAGGGT
    GTCTACGTCA ACACGTACGG GCGCATCATT AAGGATGTGG TGCTGCAGTG GGGGGAGATG
    CCTACTTCTG TGGCCTACAT CTGCTCCAAC CAGATAATGG GCTGGGGTGA GAAAGCCATT
    GAGATCCGCT CTGTGGAGAC GGGCCACCTC GACGGGGTCT TCATGCACAA ACGAGCTCAG
    AGGCTCAAGT TCCTGTGTGA GCGGAATGAC AAGGTGTTTT TTGCCTCAGT CCGCTCTGGG
    GGCAGCAGCC AAGTTTACTT CATGACTCTG AACCGTAACT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86903
    Clone ID Related Accession (Same CDS sequence) XM_017024705.1
    Accession Version XM_017024705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCAGCGATGG GGATACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTC
    GAGGAGATCA CCGGGACCCA GCCCCCATAC GGGGGCGGCA CCATGGTGGT CCAGCGCACC
    CCTGAAGAGG AGCGGAACCT GCTGCATGCT GACAGCAATG GGTACACAAA CCTGCCTGAC
    GTGGTCCAGC CCAGCCACTC ACCCACCGAG AACAGCAAAG GCCAAAGCCC ACCCTCGAAG
    GATGGGAGTG GTGACTACCA GTCTCGTGGG CTGGTAAAGG CCCCTGGCAA GAGCTCGTTC
    ACGATGTTTG TGGATCTAGG GATCTACCAG CCTGGAGGCA GTGGGGACAG CATCCCCATC
    ACAGCCCTAG TGGGTGGAGA GGGCACTCGG CTCGACCAGC TGCAGTACGA CGTGAGGAAG
    GGTTCTGTGG TCAACGTGAA TCCCACCAAC ACCCGGGCCC ACAGTGAGAC CCCTGAGATC
    CGGAAGTACA AGAAGCGATT CAACTCCGAG ATCCTCTGTG CAGCCCTTTG GGGGGTCAAC
    CTGCTGGTGG GCACGGAGAA CGGGCTGATG TTGCTGGACC GAAGTGGGCA GGGCAAGGTG
    TATGGACTCA TTGGGCGGCG ACGCTTCCAG CAGATGGATG TGCTGGAGGG GCTCAACCTG
    CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG
    AACAAGATTC TGCACAATGA CCCAGAAGTG GAGAAGAAGC AGGGCTGGAC CACCGTGGGG
    GACATGGAGG GCTGCGGGCA CTACCGTGTT GTGAAATACG AGCGGATTAA GTTCCTGGTC
    ATCGCCCTCA AGAGCTCCGT GGAGGTGTAT GCCTGGGCCC CCAAACCCTA CCACAAATTC
    ATGGCCTTCA AGTCCTTTGC CGACCTCCCC CACCGCCCTC TGCTGGTCGA CCTGACAGTA
    GAGGAGGGGC AGCGGCTCAA GGTCATCTAT GGCTCCAGTG CTGGCTTCCA TGCTGTGGAT
    GTCGACTCGG GGAACAGCTA TGACATCTAC ATCCCTGTGC ACATCCAGAG CCAGATCACG
    CCCCATGCCA TCATCTTCCT CCCCAACACC GACGGCATGG AGATGCTGCT GTGCTACGAG
    GACGAGGGTG TCTACGTCAA CACGTACGGG CGCATCATTA AGGATGTGGT GCTGCAGTGG
    GGGGAGATGC CTACTTCTGT GGCCTACATC TGCTCCAACC AGATAATGGG CTGGGGTGAG
    AAAGCCATTG AGATCCGCTC TGTGGAGACG GGCCACCTCG ACGGGGTCTT CATGCACAAA
    CGAGCTCAGA GGCTCAAGTT CCTGTGTGAG CGGAATGACA AGGTGTTTTT TGCCTCAGTC
    CGCTCTGGGG GCAGCAGCCA AGTTTACTTC ATGACTCTGA ACCGTAACTG CATCATGAAC
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880194.1
    CDS245..4270
    Translation

    Target ORF information:

    RefSeq Version XM_017024705.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGACCTCG CAGCAACTCC GCCTGGCAAA TCTATCTGCA AAGGCGGGCA
    GAGCGGGGCA CCCCAAAGCC TCCAGGGCCC CCTGCTCAGC CCCCTGGCCC GCCCAACGCC
    TCTAGTAACC CCGACCTCAG GAGGAGCGAC CCTGGCTGGG AACGCTCGGA CAGCGTCCTT
    CCAGCCTCTC ACGGGCACCT CCCCCAGGCT GGCTCACTGG AGCGGAACCG CGTGGGAGTC
    TCCTCCAAAC CGGACAGCTC CCCTGTGCTC TCCCCTGGGA ATAAAGCCAA GCCCGACGAC
    CACCGCTCAC GGCCAGGCCG GCCCGCAGAC TTTGTGTTGC TGAAAGAGCG GACTCTGGAC
    GAGGCCCCTC GGCCTCCCAA GAAGGCCATG GACTACTCGT CGTCCAGCGA GGAGGTGGAA
    AGCAGTGAGG ACGACGAGGA GGAAGGCGAA GGCGGGCCAG CAGAGGGGAG CAGAGATACC
    CCTGGGGGCC GCAGCGATGG GGATACAGAC AGCGTCAGCA CCATGGTGGT CCACGACGTC
    GAGGAGATCA CCGGGACCCA GCCCCCATAC GGGGGCGGCA CCATGGTGGT CCAGCGCACC
    CCTGAAGAGG AGCGGAACCT GCTGCATGCT GACAGCAATG GGTACACAAA CCTGCCTGAC
    GTGGTCCAGC CCAGCCACTC ACCCACCGAG AACAGCAAAG GCCAAAGCCC ACCCTCGAAG
    GATGGGAGTG GTGACTACCA GTCTCGTGGG CTGGTAAAGG CCCCTGGCAA GAGCTCGTTC
    ACGATGTTTG TGGATCTAGG GATCTACCAG CCTGGAGGCA GTGGGGACAG CATCCCCATC
    ACAGCCCTAG TGGGTGGAGA GGGCACTCGG CTCGACCAGC TGCAGTACGA CGTGAGGAAG
    GGTTCTGTGG TCAACGTGAA TCCCACCAAC ACCCGGGCCC ACAGTGAGAC CCCTGAGATC
    CGGAAGTACA AGAAGCGATT CAACTCCGAG ATCCTCTGTG CAGCCCTTTG GGGGGTCAAC
    CTGCTGGTGG GCACGGAGAA CGGGCTGATG TTGCTGGACC GAAGTGGGCA GGGCAAGGTG
    TATGGACTCA TTGGGCGGCG ACGCTTCCAG CAGATGGATG TGCTGGAGGG GCTCAACCTG
    CTCATCACCA TCTCAGGGAA AAGGAACAAA CTGCGGGTGT ATTACCTGTC CTGGCTCCGG
    AACAAGATTC TGCACAATGA CCCAGAAGTG GAGAAGAAGC AGGGCTGGAC CACCGTGGGG
    GACATGGAGG GCTGCGGGCA CTACCGTGTT GTGAAATACG AGCGGATTAA GTTCCTGGTC
    ATCGCCCTCA AGAGCTCCGT GGAGGTGTAT GCCTGGGCCC CCAAACCCTA CCACAAATTC
    ATGGCCTTCA AGTCCTTTGC CGACCTCCCC CACCGCCCTC TGCTGGTCGA CCTGACAGTA
    GAGGAGGGGC AGCGGCTCAA GGTCATCTAT GGCTCCAGTG CTGGCTTCCA TGCTGTGGAT
    GTCGACTCGG GGAACAGCTA TGACATCTAC ATCCCTGTGC ACATCCAGAG CCAGATCACG
    CCCCATGCCA TCATCTTCCT CCCCAACACC GACGGCATGG AGATGCTGCT GTGCTACGAG
    GACGAGGGTG TCTACGTCAA CACGTACGGG CGCATCATTA AGGATGTGGT GCTGCAGTGG
    GGGGAGATGC CTACTTCTGT GGCCTACATC TGCTCCAACC AGATAATGGG CTGGGGTGAG
    AAAGCCATTG AGATCCGCTC TGTGGAGACG GGCCACCTCG ACGGGGTCTT CATGCACAAA
    CGAGCTCAGA GGCTCAAGTT CCTGTGTGAG CGGAATGACA AGGTGTTTTT TGCCTCAGTC
    CGCTCTGGGG GCAGCAGCCA AGTTTACTTC ATGACTCTGA ACCGTAACTG CATCATGAAC
    TGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86904
    Clone ID Related Accession (Same CDS sequence) XM_017024706.1
    Accession Version XM_017024706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGTAACCC CGACCTCAGG AGGAGCGACC CTGGCTGGGA ACGCTCGGAC
    AGCGTCCTTC CAGCCTCTCA CGGGCACCTC CCCCAGGCTG GCTCACTGGA GCGGAACCGC
    GTGGGAGTCT CCTCCAAACC GGACAGCTCC CCTGTGCTCT CCCCTGGGAA TAAAGCCAAG
    CCCGACGACC ACCGCTCACG GCCAGGCCGG CCCGCAGACT TTGTGTTGCT GAAAGAGCGG
    ACTCTGGACG AGGCCCCTCG GCCTCCCAAG AAGGCCATGG ACTACTCGTC GTCCAGCGAG
    GAGGTGGAAA GCAGTGAGGA CGACGAGGAG GAAGGCGAAG GCGGGCCAGC AGAGGGGAGC
    AGAGATACCC CTGGGGGCCG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880195.1
    CDS245..4216
    Translation

    Target ORF information:

    RefSeq Version XM_017024706.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGGCGACC CAGCCCCCGC CCGCAGCCTG GACGACATCG ACCTGTCCGC CCTGCGGGAC 
    CCTGCTGGGA TCTTTGAGCT TGTGGAGGTG GTCGGCAATG GAACCTACGG ACAGGTGTAC
    AAGGGTCGGC ATGTCAAGAC GGGGCAGCTG GCTGCCATCA AGGTCATGGA TGTCACGGAG
    GACGAGGAGG AAGAGATCAA ACAGGAGATC AACATGCTGA AAAAGTACTC TCACCACCGC
    AACATCGCCA CCTACTACGG AGCCTTCATC AAGAAGAGCC CCCCGGGAAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGTTCAGTGA CTGACCTGGT AAAGAACACA
    AAAGGCAACG CCCTGAAGGA GGACTGTATC GCCTATATCT GCAGGGAGAT CCTCAGGGGT
    CTGGCCCATC TCCATGCCCA CAAGGTGATC CATCGAGACA TCAAGGGGCA GAATGTGCTG
    CTGACAGAGA ATGCTGAGGT CAAGCTAGTG GATTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACCGTGGGCA GACGGAACAC TTTCATTGGG ACTCCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCTGA TGCCACCTAT GATTACAGGA GTGATATTTG GTCTCTAGGA
    ATCACAGCCA TCGAGATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCCATGCGA
    GCCCTCTTCC TCATTCCTCG GAACCCTCCG CCCAGGCTCA AGTCCAAGAA GTGGTCTAAG
    AAGTTCATTG ACTTCATTGA CACATGTCTC ATCAAGACTT ACCTGAGCCG CCCACCCACG
    GAGCAGCTAC TGAAGTTTCC CTTCATCCGG GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTTAAGG ACCACATTGA CCGATCCCGG AAGAAGCGGG GTGAGAAAGA GGAGACAGAA
    TATGAGTACA GCGGCAGCGA GGAGGAAGAT GACAGCCATG GAGAGGAAGG AGAGCCAAGC
    TCCATCATGA ACGTGCCTGG AGAGTCGACT CTACGCCGGG AGTTTCTCCG GCTCCAGCAG
    GAAAATAAGA GCAACTCAGA GGCTTTAAAA CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGAGACCCCG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGGCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGCCG CGTGGAGGAG CAACAGCGGC GGGAGCGGGA GCAGCGGAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGCTG GAGGACATGC AGGCTCTGCG GCGGGAGGAG
    GAGCGGCGGC AGGCGGAGCG CGAGCAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCCTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCAGAACGTC TCCAGAGGCA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAACAGC AGCAGCAGCT TCAGAAACAG
    CAGCAGCAGC AGCTCCTGCC TGGGGACAGG AAGCCCCTGT ACCATTATGG TCGGGGCATG
    AATCCCGCTG ACAAACCAGC CTGGGCCCGA GAGGTAGAAG AGAGAACAAG GATGAACAAG
    CAGCAGAACT CTCCCTTGGC CAAGAGCAAG CCAGGCAGCA CGGGGCCTGA GCCCCCCATC
    CCCCAGGCCT CCCCAGGGCC CCCAGGACCC CTTTCCCAGA CTCCTCCTAT GCAGAGGCCG
    GTGGAGCCCC AGGAGGGACC GCACAAGAGC CTGGTGGCAC ACCGGGTCCC ACTGAAGCCA
    TATGCAGCAC CTGTACCCCG ATCCCAGTCC CTGCAGGACC AGCCCACCCG AAACCTGGCT
    GCCTTCCCAG CCTCCCATGA CCCCGACCCT GCCATCCCCG CACCCACTGC CACGCCCAGT
    GCCCGAGGAG CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGACC TGGCCCCAGC
    CCGAATCCCC CAGCCTGGGT CCGCCCAGAT AACGAGGCCC CACCCAAGGT GCCTCAGAGG
    ACCTCATCTA TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCCCAG
    GCAGTCCGTG CCAGTAACCC CGACCTCAGG AGGAGCGACC CTGGCTGGGA ACGCTCGGAC
    AGCGTCCTTC CAGCCTCTCA CGGGCACCTC CCCCAGGCTG GCTCACTGGA GCGGAACCGC
    GTGGGAGTCT CCTCCAAACC GGACAGCTCC CCTGTGCTCT CCCCTGGGAA TAAAGCCAAG
    CCCGACGACC ACCGCTCACG GCCAGGCCGG CCCGCAGACT TTGTGTTGCT GAAAGAGCGG
    ACTCTGGACG AGGCCCCTCG GCCTCCCAAG AAGGCCATGG ACTACTCGTC GTCCAGCGAG
    GAGGTGGAAA GCAGTGAGGA CGACGAGGAG GAAGGCGAAG GCGGGCCAGC AGAGGGGAGC
    AGAGATACCC CTGGGGGCCG CGATGGGGAT ACAGACAGCG TCAGCACCAT GGTGGTCCAC
    GACGTCGAGG AGATCACCGG GACCCAGCCC CCATACGGGG GCGGCACCAT GGTGGTCCAG
    CGCACCCCTG AAGAGGAGCG GAACCTGCTG CATGCTGACA GCAATGGGTA CACAAACCTG
    CCTGACGTGG TCCAGCCCAG CCACTCACCC ACCGAGAACA GCAAAGGCCA AAGCCCACCC
    TCGAAGGATG GGAGTGGTGA CTACCAGTCT CGTGGGCTGG TAAAGGCCCC TGGCAAGAGC
    TCGTTCACGA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGACAGCATC
    CCCATCACAG CCCTAGTGGG TGGAGAGGGC ACTCGGCTCG ACCAGCTGCA GTACGACGTG
    AGGAAGGGTT CTGTGGTCAA CGTGAATCCC ACCAACACCC GGGCCCACAG TGAGACCCCT
    GAGATCCGGA AGTACAAGAA GCGATTCAAC TCCGAGATCC TCTGTGCAGC CCTTTGGGGG
    GTCAACCTGC TGGTGGGCAC GGAGAACGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAGGTGTATG GACTCATTGG GCGGCGACGC TTCCAGCAGA TGGATGTGCT GGAGGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTGTATTA CCTGTCCTGG
    CTCCGGAACA AGATTCTGCA CAATGACCCA GAAGTGGAGA AGAAGCAGGG CTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGGCACTAC CGTGTTGTGA AATACGAGCG GATTAAGTTC
    CTGGTCATCG CCCTCAAGAG CTCCGTGGAG GTGTATGCCT GGGCCCCCAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCCGAC CTCCCCCACC GCCCTCTGCT GGTCGACCTG
    ACAGTAGAGG AGGGGCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTCG ACTCGGGGAA CAGCTATGAC ATCTACATCC CTGTGCACAT CCAGAGCCAG
    ATCACGCCCC ATGCCATCAT CTTCCTCCCC AACACCGACG GCATGGAGAT GCTGCTGTGC
    TACGAGGACG AGGGTGTCTA CGTCAACACG TACGGGCGCA TCATTAAGGA TGTGGTGCTG
    CAGTGGGGGG AGATGCCTAC TTCTGTGGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAAG CCATTGAGAT CCGCTCTGTG GAGACGGGCC ACCTCGACGG GGTCTTCATG
    CACAAACGAG CTCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGACAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGGGGCAG CAGCCAAGTT TACTTCATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86905
    Clone ID Related Accession (Same CDS sequence) XM_017024707.2 , XM_017024707.1
    Accession Version XM_017024707.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017024707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    CTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCAGA ACGTCTCCAG
    AGGCAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA ACAGCAGCAG
    CAGCTTCAGA AACAGCAGCA GCAGCAGCTC CTGCCTGGGG ACAGGAAGCC CCTGTACCAT
    TATGGTCGGG GCATGAATCC CGCTGACAAA CCAGCCTGGG CCCGAGAGGT AGAAGAGAGA
    ACAAGGATGA ACAAGCAGCA GAACTCTCCC TTGGCCAAGA GCAAGCCAGG CAGCACGGGG
    CCTGAGCCCC CCATCCCCCA GGCCTCCCCA GGGCCCCCAG GACCCCTTTC CCAGACTCCT
    CCTATGCAGA GGCCGGTGGA GCCCCAGGAG GGACCGCACA AGAGCCTGGT GGCACACCGG
    GTCCCACTGA AGCCATATGC AGCACCTGTA CCCCGATCCC AGTCCCTGCA GGACCAGCCC
    ACCCGAAACC TGGCTGCCTT CCCAGCCTCC CATGACCCCG ACCCTGCCAT CCCCGCACCC
    ACTGCCACGC CCAGTGCCCG AGGAGCTGTC ATCCGCCAGA ATTCAGACCC CACCTCTGAA
    GGACCTGGCC CCAGCCCGAA TCCCCCAGCC TGGGTCCGCC CAGATAACGA GGCCCCACCC
    AAGGTGCCTC AGAGGACCTC ATCTATCGCC ACTGCCCTTA ACACCAGTGG GGCCGGAGGG
    TCCCGGCCAG CCCAGGCAGT CCGTGCCAGA CCTCGCAGCA ACTCCGCCTG GCAAATCTAT
    CTGCAAAGGC GGGCAGAGCG GGGCACCCCA AAGCCTCCAG GGCCCCCTGC TCAGCCCCCT
    GGCCCGCCCA ACGCCTCTAG TAACCCCGAC CTCAGGAGGA GCGACCCTGG CTGGGAACGC
    TCGGACAGCG TCCTTCCAGC CTCTCACGGG CACCTCCCCC AGGCTGGCTC ACTGGAGCGG
    AACCGCGTGG GAGTCTCCTC CAAACCGGAC AGCTCCCCTG TGCTCTCCCC TGGGAATAAA
    GCCAAGCCCG ACGACCACCG CTCACGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTGAAA
    GAGCGGACTC TGGACGAGGC CCCTCGGCCT CCCAAGAAGG CCATGGACTA CTCGTCGTCC
    AGCGAGGAGG TGGAAAGCAG TGAGGACGAC GAGGAGGAAG GCGAAGGCGG GCCAGCAGAG
    GGGAGCAGAG ATACCCCTGG GGGCCGCAGC GATGGGGATA CAGACAGCGT CAGCACCATG
    GTGGTCCACG ACGTCGAGGA GATCACCGGG ACCCAGCCCC CATACGGGGG CGGCACCATG
    GTGGTCCAGC GCACCCCTGA AGAGGAGCGG AACCTGCTGC ATGCTGACAG CAATGGGTAC
    ACAAACCTGC CTGACGTGGT CCAGCCCAGC CACTCACCCA CCGAGAACAG CAAAGGCCAA
    AGCCCACCCT CGAAGGATGG GAGTGGTGAC TACCAGTCTC GTGGGCTGGT AAAGGCCCCT
    GGCAAGAGCT CGTTCACGAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG
    GACAGCATCC CCATCACAGC CCTAGTGGGT GGAGAGGGCA CTCGGCTCGA CCAGCTGCAG
    TACGACGTGA GGAAGGGTTC TGTGGTCAAC GTGAATCCCA CCAACACCCG GGCCCACAGT
    GAGACCCCTG AGATCCGGAA GTACAAGAAG CGATTCAACT CCGAGATCCT CTGTGCAGCC
    CTTTGGGGGG TCAACCTGCT GGTGGGCACG GAGAACGGGC TGATGTTGCT GGACCGAAGT
    GGGCAGGGCA AGGTGTATGG ACTCATTGGG CGGCGACGCT TCCAGCAGAT GGATGTGCTG
    GAGGGGCTCA ACCTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTGTATTAC
    CTGTCCTGGC TCCGGAACAA GATTCTGCAC AATGACCCAG AAGTGGAGAA GAAGCAGGGC
    TGGACCACCG TGGGGGACAT GGAGGGCTGC GGGCACTACC GTGTTGTGAA ATACGAGCGG
    ATTAAGTTCC TGGTCATCGC CCTCAAGAGC TCCGTGGAGG TGTATGCCTG GGCCCCCAAA
    CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCCGACC TCCCCCACCG CCCTCTGCTG
    GTCGACCTGA CAGTAGAGGA GGGGCAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC
    TTCCATGCTG TGGATGTCGA CTCGGGGAAC AGCTATGACA TCTACATCCC TGTGCACATC
    CAGAGCCAGA TCACGCCCCA TGCCATCATC TTCCTCCCCA ACACCGACGG CATGGAGATG
    CTGCTGTGCT ACGAGGACGA GGGTGTCTAC GTCAACACGT ACGGGCGCAT CATTAAGGAT
    GTGGTGCTGC AGTGGGGGGA GATGCCTACT TCTGTGGCCT ACATCTGCTC CAACCAGATA
    ATGGGCTGGG GTGAGAAAGC CATTGAGATC CGCTCTGTGG AGACGGGCCA CCTCGACGGG
    GTCTTCATGC ACAAACGAGC TCAGAGGCTC AAGTTCCTGT GTGAGCGGAA TGACAAGGTG
    TTTTTTGCCT CAGTCCGCTC TGGGGGCAGC AGCCAAGTTT ACTTCATGAC TCTGAACCGT
    AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880196.1
    CDS212..4132
    Translation

    Target ORF information:

    RefSeq Version XM_017024707.2
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024707.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    CTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCAGA ACGTCTCCAG
    AGGCAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA ACAGCAGCAG
    CAGCTTCAGA AACAGCAGCA GCAGCAGCTC CTGCCTGGGG ACAGGAAGCC CCTGTACCAT
    TATGGTCGGG GCATGAATCC CGCTGACAAA CCAGCCTGGG CCCGAGAGGT AGAAGAGAGA
    ACAAGGATGA ACAAGCAGCA GAACTCTCCC TTGGCCAAGA GCAAGCCAGG CAGCACGGGG
    CCTGAGCCCC CCATCCCCCA GGCCTCCCCA GGGCCCCCAG GACCCCTTTC CCAGACTCCT
    CCTATGCAGA GGCCGGTGGA GCCCCAGGAG GGACCGCACA AGAGCCTGGT GGCACACCGG
    GTCCCACTGA AGCCATATGC AGCACCTGTA CCCCGATCCC AGTCCCTGCA GGACCAGCCC
    ACCCGAAACC TGGCTGCCTT CCCAGCCTCC CATGACCCCG ACCCTGCCAT CCCCGCACCC
    ACTGCCACGC CCAGTGCCCG AGGAGCTGTC ATCCGCCAGA ATTCAGACCC CACCTCTGAA
    GGACCTGGCC CCAGCCCGAA TCCCCCAGCC TGGGTCCGCC CAGATAACGA GGCCCCACCC
    AAGGTGCCTC AGAGGACCTC ATCTATCGCC ACTGCCCTTA ACACCAGTGG GGCCGGAGGG
    TCCCGGCCAG CCCAGGCAGT CCGTGCCAGA CCTCGCAGCA ACTCCGCCTG GCAAATCTAT
    CTGCAAAGGC GGGCAGAGCG GGGCACCCCA AAGCCTCCAG GGCCCCCTGC TCAGCCCCCT
    GGCCCGCCCA ACGCCTCTAG TAACCCCGAC CTCAGGAGGA GCGACCCTGG CTGGGAACGC
    TCGGACAGCG TCCTTCCAGC CTCTCACGGG CACCTCCCCC AGGCTGGCTC ACTGGAGCGG
    AACCGCGTGG GAGTCTCCTC CAAACCGGAC AGCTCCCCTG TGCTCTCCCC TGGGAATAAA
    GCCAAGCCCG ACGACCACCG CTCACGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTGAAA
    GAGCGGACTC TGGACGAGGC CCCTCGGCCT CCCAAGAAGG CCATGGACTA CTCGTCGTCC
    AGCGAGGAGG TGGAAAGCAG TGAGGACGAC GAGGAGGAAG GCGAAGGCGG GCCAGCAGAG
    GGGAGCAGAG ATACCCCTGG GGGCCGCAGC GATGGGGATA CAGACAGCGT CAGCACCATG
    GTGGTCCACG ACGTCGAGGA GATCACCGGG ACCCAGCCCC CATACGGGGG CGGCACCATG
    GTGGTCCAGC GCACCCCTGA AGAGGAGCGG AACCTGCTGC ATGCTGACAG CAATGGGTAC
    ACAAACCTGC CTGACGTGGT CCAGCCCAGC CACTCACCCA CCGAGAACAG CAAAGGCCAA
    AGCCCACCCT CGAAGGATGG GAGTGGTGAC TACCAGTCTC GTGGGCTGGT AAAGGCCCCT
    GGCAAGAGCT CGTTCACGAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG
    GACAGCATCC CCATCACAGC CCTAGTGGGT GGAGAGGGCA CTCGGCTCGA CCAGCTGCAG
    TACGACGTGA GGAAGGGTTC TGTGGTCAAC GTGAATCCCA CCAACACCCG GGCCCACAGT
    GAGACCCCTG AGATCCGGAA GTACAAGAAG CGATTCAACT CCGAGATCCT CTGTGCAGCC
    CTTTGGGGGG TCAACCTGCT GGTGGGCACG GAGAACGGGC TGATGTTGCT GGACCGAAGT
    GGGCAGGGCA AGGTGTATGG ACTCATTGGG CGGCGACGCT TCCAGCAGAT GGATGTGCTG
    GAGGGGCTCA ACCTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTGTATTAC
    CTGTCCTGGC TCCGGAACAA GATTCTGCAC AATGACCCAG AAGTGGAGAA GAAGCAGGGC
    TGGACCACCG TGGGGGACAT GGAGGGCTGC GGGCACTACC GTGTTGTGAA ATACGAGCGG
    ATTAAGTTCC TGGTCATCGC CCTCAAGAGC TCCGTGGAGG TGTATGCCTG GGCCCCCAAA
    CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCCGACC TCCCCCACCG CCCTCTGCTG
    GTCGACCTGA CAGTAGAGGA GGGGCAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC
    TTCCATGCTG TGGATGTCGA CTCGGGGAAC AGCTATGACA TCTACATCCC TGTGCACATC
    CAGAGCCAGA TCACGCCCCA TGCCATCATC TTCCTCCCCA ACACCGACGG CATGGAGATG
    CTGCTGTGCT ACGAGGACGA GGGTGTCTAC GTCAACACGT ACGGGCGCAT CATTAAGGAT
    GTGGTGCTGC AGTGGGGGGA GATGCCTACT TCTGTGGCCT ACATCTGCTC CAACCAGATA
    ATGGGCTGGG GTGAGAAAGC CATTGAGATC CGCTCTGTGG AGACGGGCCA CCTCGACGGG
    GTCTTCATGC ACAAACGAGC TCAGAGGCTC AAGTTCCTGT GTGAGCGGAA TGACAAGGTG
    TTTTTTGCCT CAGTCCGCTC TGGGGGCAGC AGCCAAGTTT ACTTCATGAC TCTGAACCGT
    AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86905
    Clone ID Related Accession (Same CDS sequence) XM_017024707.2 , XM_017024707.1
    Accession Version XM_017024707.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    CTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCAGA ACGTCTCCAG
    AGGCAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA ACAGCAGCAG
    CAGCTTCAGA AACAGCAGCA GCAGCAGCTC CTGCCTGGGG ACAGGAAGCC CCTGTACCAT
    TATGGTCGGG GCATGAATCC CGCTGACAAA CCAGCCTGGG CCCGAGAGGT AGAAGAGAGA
    ACAAGGATGA ACAAGCAGCA GAACTCTCCC TTGGCCAAGA GCAAGCCAGG CAGCACGGGG
    CCTGAGCCCC CCATCCCCCA GGCCTCCCCA GGGCCCCCAG GACCCCTTTC CCAGACTCCT
    CCTATGCAGA GGCCGGTGGA GCCCCAGGAG GGACCGCACA AGAGCCTGGT GGCACACCGG
    GTCCCACTGA AGCCATATGC AGCACCTGTA CCCCGATCCC AGTCCCTGCA GGACCAGCCC
    ACCCGAAACC TGGCTGCCTT CCCAGCCTCC CATGACCCCG ACCCTGCCAT CCCCGCACCC
    ACTGCCACGC CCAGTGCCCG AGGAGCTGTC ATCCGCCAGA ATTCAGACCC CACCTCTGAA
    GGACCTGGCC CCAGCCCGAA TCCCCCAGCC TGGGTCCGCC CAGATAACGA GGCCCCACCC
    AAGGTGCCTC AGAGGACCTC ATCTATCGCC ACTGCCCTTA ACACCAGTGG GGCCGGAGGG
    TCCCGGCCAG CCCAGGCAGT CCGTGCCAGA CCTCGCAGCA ACTCCGCCTG GCAAATCTAT
    CTGCAAAGGC GGGCAGAGCG GGGCACCCCA AAGCCTCCAG GGCCCCCTGC TCAGCCCCCT
    GGCCCGCCCA ACGCCTCTAG TAACCCCGAC CTCAGGAGGA GCGACCCTGG CTGGGAACGC
    TCGGACAGCG TCCTTCCAGC CTCTCACGGG CACCTCCCCC AGGCTGGCTC ACTGGAGCGG
    AACCGCGTGG GAGTCTCCTC CAAACCGGAC AGCTCCCCTG TGCTCTCCCC TGGGAATAAA
    GCCAAGCCCG ACGACCACCG CTCACGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTGAAA
    GAGCGGACTC TGGACGAGGC CCCTCGGCCT CCCAAGAAGG CCATGGACTA CTCGTCGTCC
    AGCGAGGAGG TGGAAAGCAG TGAGGACGAC GAGGAGGAAG GCGAAGGCGG GCCAGCAGAG
    GGGAGCAGAG ATACCCCTGG GGGCCGCAGC GATGGGGATA CAGACAGCGT CAGCACCATG
    GTGGTCCACG ACGTCGAGGA GATCACCGGG ACCCAGCCCC CATACGGGGG CGGCACCATG
    GTGGTCCAGC GCACCCCTGA AGAGGAGCGG AACCTGCTGC ATGCTGACAG CAATGGGTAC
    ACAAACCTGC CTGACGTGGT CCAGCCCAGC CACTCACCCA CCGAGAACAG CAAAGGCCAA
    AGCCCACCCT CGAAGGATGG GAGTGGTGAC TACCAGTCTC GTGGGCTGGT AAAGGCCCCT
    GGCAAGAGCT CGTTCACGAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG
    GACAGCATCC CCATCACAGC CCTAGTGGGT GGAGAGGGCA CTCGGCTCGA CCAGCTGCAG
    TACGACGTGA GGAAGGGTTC TGTGGTCAAC GTGAATCCCA CCAACACCCG GGCCCACAGT
    GAGACCCCTG AGATCCGGAA GTACAAGAAG CGATTCAACT CCGAGATCCT CTGTGCAGCC
    CTTTGGGGGG TCAACCTGCT GGTGGGCACG GAGAACGGGC TGATGTTGCT GGACCGAAGT
    GGGCAGGGCA AGGTGTATGG ACTCATTGGG CGGCGACGCT TCCAGCAGAT GGATGTGCTG
    GAGGGGCTCA ACCTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTGTATTAC
    CTGTCCTGGC TCCGGAACAA GATTCTGCAC AATGACCCAG AAGTGGAGAA GAAGCAGGGC
    TGGACCACCG TGGGGGACAT GGAGGGCTGC GGGCACTACC GTGTTGTGAA ATACGAGCGG
    ATTAAGTTCC TGGTCATCGC CCTCAAGAGC TCCGTGGAGG TGTATGCCTG GGCCCCCAAA
    CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCCGACC TCCCCCACCG CCCTCTGCTG
    GTCGACCTGA CAGTAGAGGA GGGGCAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC
    TTCCATGCTG TGGATGTCGA CTCGGGGAAC AGCTATGACA TCTACATCCC TGTGCACATC
    CAGAGCCAGA TCACGCCCCA TGCCATCATC TTCCTCCCCA ACACCGACGG CATGGAGATG
    CTGCTGTGCT ACGAGGACGA GGGTGTCTAC GTCAACACGT ACGGGCGCAT CATTAAGGAT
    GTGGTGCTGC AGTGGGGGGA GATGCCTACT TCTGTGGCCT ACATCTGCTC CAACCAGATA
    ATGGGCTGGG GTGAGAAAGC CATTGAGATC CGCTCTGTGG AGACGGGCCA CCTCGACGGG
    GTCTTCATGC ACAAACGAGC TCAGAGGCTC AAGTTCCTGT GTGAGCGGAA TGACAAGGTG
    TTTTTTGCCT CAGTCCGCTC TGGGGGCAGC AGCCAAGTTT ACTTCATGAC TCTGAACCGT
    AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880196.1
    CDS211..4131
    Translation

    Target ORF information:

    RefSeq Version XM_017024707.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATGTCA CGGAGGACGA GGAGGAAGAG ATCAAACAGG AGATCAACAT GCTGAAAAAG 
    TACTCTCACC ACCGCAACAT CGCCACCTAC TACGGAGCCT TCATCAAGAA GAGCCCCCCG
    GGAAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGTTC AGTGACTGAC
    CTGGTAAAGA ACACAAAAGG CAACGCCCTG AAGGAGGACT GTATCGCCTA TATCTGCAGG
    GAGATCCTCA GGGGTCTGGC CCATCTCCAT GCCCACAAGG TGATCCATCG AGACATCAAG
    GGGCAGAATG TGCTGCTGAC AGAGAATGCT GAGGTCAAGC TAGTGGATTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACCGT GGGCAGACGG AACACTTTCA TTGGGACTCC CTACTGGATG
    GCTCCAGAGG TCATCGCCTG TGATGAGAAC CCTGATGCCA CCTATGATTA CAGGAGTGAT
    ATTTGGTCTC TAGGAATCAC AGCCATCGAG ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCCA TGCGAGCCCT CTTCCTCATT CCTCGGAACC CTCCGCCCAG GCTCAAGTCC
    AAGAAGTGGT CTAAGAAGTT CATTGACTTC ATTGACACAT GTCTCATCAA GACTTACCTG
    AGCCGCCCAC CCACGGAGCA GCTACTGAAG TTTCCCTTCA TCCGGGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT TAAGGACCAC ATTGACCGAT CCCGGAAGAA GCGGGGTGAG
    AAAGAGGAGA CAGAATATGA GTACAGCGGC AGCGAGGAGG AAGATGACAG CCATGGAGAG
    GAAGGAGAGC CAAGCTCCAT CATGAACGTG CCTGGAGAGT CGACTCTACG CCGGGAGTTT
    CTCCGGCTCC AGCAGGAAAA TAAGAGCAAC TCAGAGGCTT TAAAACAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGAGA CCCCGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGGCGCATAG AGGAGCAGAA GGAGGAGCGG CGCCGCGTGG AGGAGCAACA GCGGCGGGAG
    CGGGAGCAGC GGAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGCTGGAGGA CATGCAGGCT
    CTGCGGCGGG AGGAGGAGCG GCGGCAGGCG GAGCGCGAGC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    CTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCAGA ACGTCTCCAG
    AGGCAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA ACAGCAGCAG
    CAGCTTCAGA AACAGCAGCA GCAGCAGCTC CTGCCTGGGG ACAGGAAGCC CCTGTACCAT
    TATGGTCGGG GCATGAATCC CGCTGACAAA CCAGCCTGGG CCCGAGAGGT AGAAGAGAGA
    ACAAGGATGA ACAAGCAGCA GAACTCTCCC TTGGCCAAGA GCAAGCCAGG CAGCACGGGG
    CCTGAGCCCC CCATCCCCCA GGCCTCCCCA GGGCCCCCAG GACCCCTTTC CCAGACTCCT
    CCTATGCAGA GGCCGGTGGA GCCCCAGGAG GGACCGCACA AGAGCCTGGT GGCACACCGG
    GTCCCACTGA AGCCATATGC AGCACCTGTA CCCCGATCCC AGTCCCTGCA GGACCAGCCC
    ACCCGAAACC TGGCTGCCTT CCCAGCCTCC CATGACCCCG ACCCTGCCAT CCCCGCACCC
    ACTGCCACGC CCAGTGCCCG AGGAGCTGTC ATCCGCCAGA ATTCAGACCC CACCTCTGAA
    GGACCTGGCC CCAGCCCGAA TCCCCCAGCC TGGGTCCGCC CAGATAACGA GGCCCCACCC
    AAGGTGCCTC AGAGGACCTC ATCTATCGCC ACTGCCCTTA ACACCAGTGG GGCCGGAGGG
    TCCCGGCCAG CCCAGGCAGT CCGTGCCAGA CCTCGCAGCA ACTCCGCCTG GCAAATCTAT
    CTGCAAAGGC GGGCAGAGCG GGGCACCCCA AAGCCTCCAG GGCCCCCTGC TCAGCCCCCT
    GGCCCGCCCA ACGCCTCTAG TAACCCCGAC CTCAGGAGGA GCGACCCTGG CTGGGAACGC
    TCGGACAGCG TCCTTCCAGC CTCTCACGGG CACCTCCCCC AGGCTGGCTC ACTGGAGCGG
    AACCGCGTGG GAGTCTCCTC CAAACCGGAC AGCTCCCCTG TGCTCTCCCC TGGGAATAAA
    GCCAAGCCCG ACGACCACCG CTCACGGCCA GGCCGGCCCG CAGACTTTGT GTTGCTGAAA
    GAGCGGACTC TGGACGAGGC CCCTCGGCCT CCCAAGAAGG CCATGGACTA CTCGTCGTCC
    AGCGAGGAGG TGGAAAGCAG TGAGGACGAC GAGGAGGAAG GCGAAGGCGG GCCAGCAGAG
    GGGAGCAGAG ATACCCCTGG GGGCCGCAGC GATGGGGATA CAGACAGCGT CAGCACCATG
    GTGGTCCACG ACGTCGAGGA GATCACCGGG ACCCAGCCCC CATACGGGGG CGGCACCATG
    GTGGTCCAGC GCACCCCTGA AGAGGAGCGG AACCTGCTGC ATGCTGACAG CAATGGGTAC
    ACAAACCTGC CTGACGTGGT CCAGCCCAGC CACTCACCCA CCGAGAACAG CAAAGGCCAA
    AGCCCACCCT CGAAGGATGG GAGTGGTGAC TACCAGTCTC GTGGGCTGGT AAAGGCCCCT
    GGCAAGAGCT CGTTCACGAT GTTTGTGGAT CTAGGGATCT ACCAGCCTGG AGGCAGTGGG
    GACAGCATCC CCATCACAGC CCTAGTGGGT GGAGAGGGCA CTCGGCTCGA CCAGCTGCAG
    TACGACGTGA GGAAGGGTTC TGTGGTCAAC GTGAATCCCA CCAACACCCG GGCCCACAGT
    GAGACCCCTG AGATCCGGAA GTACAAGAAG CGATTCAACT CCGAGATCCT CTGTGCAGCC
    CTTTGGGGGG TCAACCTGCT GGTGGGCACG GAGAACGGGC TGATGTTGCT GGACCGAAGT
    GGGCAGGGCA AGGTGTATGG ACTCATTGGG CGGCGACGCT TCCAGCAGAT GGATGTGCTG
    GAGGGGCTCA ACCTGCTCAT CACCATCTCA GGGAAAAGGA ACAAACTGCG GGTGTATTAC
    CTGTCCTGGC TCCGGAACAA GATTCTGCAC AATGACCCAG AAGTGGAGAA GAAGCAGGGC
    TGGACCACCG TGGGGGACAT GGAGGGCTGC GGGCACTACC GTGTTGTGAA ATACGAGCGG
    ATTAAGTTCC TGGTCATCGC CCTCAAGAGC TCCGTGGAGG TGTATGCCTG GGCCCCCAAA
    CCCTACCACA AATTCATGGC CTTCAAGTCC TTTGCCGACC TCCCCCACCG CCCTCTGCTG
    GTCGACCTGA CAGTAGAGGA GGGGCAGCGG CTCAAGGTCA TCTATGGCTC CAGTGCTGGC
    TTCCATGCTG TGGATGTCGA CTCGGGGAAC AGCTATGACA TCTACATCCC TGTGCACATC
    CAGAGCCAGA TCACGCCCCA TGCCATCATC TTCCTCCCCA ACACCGACGG CATGGAGATG
    CTGCTGTGCT ACGAGGACGA GGGTGTCTAC GTCAACACGT ACGGGCGCAT CATTAAGGAT
    GTGGTGCTGC AGTGGGGGGA GATGCCTACT TCTGTGGCCT ACATCTGCTC CAACCAGATA
    ATGGGCTGGG GTGAGAAAGC CATTGAGATC CGCTCTGTGG AGACGGGCCA CCTCGACGGG
    GTCTTCATGC ACAAACGAGC TCAGAGGCTC AAGTTCCTGT GTGAGCGGAA TGACAAGGTG
    TTTTTTGCCT CAGTCCGCTC TGGGGGCAGC AGCCAAGTTT ACTTCATGAC TCTGAACCGT
    AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86906
    Clone ID Related Accession (Same CDS sequence) XM_017024708.1
    Accession Version XM_017024708.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product misshapen-like kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTGAAAA AGTACTCTCA CCACCGCAAC ATCGCCACCT ACTACGGAGC CTTCATCAAG 
    AAGAGCCCCC CGGGAAACGA TGACCAGCTC TGGCTGGTGA TGGAGTTCTG TGGTGCTGGT
    TCAGTGACTG ACCTGGTAAA GAACACAAAA GGCAACGCCC TGAAGGAGGA CTGTATCGCC
    TATATCTGCA GGGAGATCCT CAGGGGTCTG GCCCATCTCC ATGCCCACAA GGTGATCCAT
    CGAGACATCA AGGGGCAGAA TGTGCTGCTG ACAGAGAATG CTGAGGTCAA GCTAGTGGAT
    TTTGGGGTGA GTGCTCAGCT GGACCGCACC GTGGGCAGAC GGAACACTTT CATTGGGACT
    CCCTACTGGA TGGCTCCAGA GGTCATCGCC TGTGATGAGA ACCCTGATGC CACCTATGAT
    TACAGGAGTG ATATTTGGTC TCTAGGAATC ACAGCCATCG AGATGGCAGA GGGAGCCCCC
    CCTCTGTGTG ACATGCACCC CATGCGAGCC CTCTTCCTCA TTCCTCGGAA CCCTCCGCCC
    AGGCTCAAGT CCAAGAAGTG GTCTAAGAAG TTCATTGACT TCATTGACAC ATGTCTCATC
    AAGACTTACC TGAGCCGCCC ACCCACGGAG CAGCTACTGA AGTTTCCCTT CATCCGGGAC
    CAGCCCACGG AGCGGCAGGT CCGCATCCAG CTTAAGGACC ACATTGACCG ATCCCGGAAG
    AAGCGGGGTG AGAAAGAGGA GACAGAATAT GAGTACAGCG GCAGCGAGGA GGAAGATGAC
    AGCCATGGAG AGGAAGGAGA GCCAAGCTCC ATCATGAACG TGCCTGGAGA GTCGACTCTA
    CGCCGGGAGT TTCTCCGGCT CCAGCAGGAA AATAAGAGCA ACTCAGAGGC TTTAAAACAG
    CAGCAGCAGC TGCAGCAGCA GCAGCAGCGA GACCCCGAGG CACACATCAA ACACCTGCTG
    CACCAGCGGC AGCGGCGCAT AGAGGAGCAG AAGGAGGAGC GGCGCCGCGT GGAGGAGCAA
    CAGCGGCGGG AGCGGGAGCA GCGGAAGCTG CAGGAGAAGG AGCAGCAGCG GCGGCTGGAG
    GACATGCAGG CTCTGCGGCG GGAGGAGGAG CGGCGGCAGG CGGAGCGCGA GCAGGAATAT
    ATTCGTCACA GGCTAGAGGA GGAGCAGCGA CAGCTCGAGA TCCTTCAGCA ACAGCTGCTC
    CAGGAACAGG CCCTGCTGCT GGAATACAAG CGGAAGCAGC TGGAGGAGCA GCGGCAGTCA
    GAACGTCTCC AGAGGCAGCT GCAGCAGGAG CATGCCTACC TCAAGTCCCT GCAGCAGCAG
    CAACAGCAGC AGCAGCTTCA GAAACAGCAG CAGCAGCAGC TCCTGCCTGG GGACAGGAAG
    CCCCTGTACC ATTATGGTCG GGGCATGAAT CCCGCTGACA AACCAGCCTG GGCCCGAGAG
    GTAGAAGAGA GAACAAGGAT GAACAAGCAG CAGAACTCTC CCTTGGCCAA GAGCAAGCCA
    GGCAGCACGG GGCCTGAGCC CCCCATCCCC CAGGCCTCCC CAGGGCCCCC AGGACCCCTT
    TCCCAGACTC CTCCTATGCA GAGGCCGGTG GAGCCCCAGG AGGGACCGCA CAAGAGCCTG
    GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG TACCCCGATC CCAGTCCCTG
    CAGGACCAGC CCACCCGAAA CCTGGCTGCC TTCCCAGCCT CCCATGACCC CGACCCTGCC
    ATCCCCGCAC CCACTGCCAC GCCCAGTGCC CGAGGAGCTG TCATCCGCCA GAATTCAGAC
    CCCACCTCTG AAGGACCTGG CCCCAGCCCG AATCCCCCAG CCTGGGTCCG CCCAGATAAC
    GAGGCCCCAC CCAAGGTGCC TCAGAGGACC TCATCTATCG CCACTGCCCT TAACACCAGT
    GGGGCCGGAG GGTCCCGGCC AGCCCAGGCA GTCCGTGCCA GACCTCGCAG CAACTCCGCC
    TGGCAAATCT ATCTGCAAAG GCGGGCAGAG CGGGGCACCC CAAAGCCTCC AGGGCCCCCT
    GCTCAGCCCC CTGGCCCGCC CAACGCCTCT AGTAACCCCG ACCTCAGGAG GAGCGACCCT
    GGCTGGGAAC GCTCGGACAG CGTCCTTCCA GCCTCTCACG GGCACCTCCC CCAGGCTGGC
    TCACTGGAGC GGAACCGCGT GGGAGTCTCC TCCAAACCGG ACAGCTCCCC TGTGCTCTCC
    CCTGGGAATA AAGCCAAGCC CGACGACCAC CGCTCACGGC CAGGCCGGCC CGCAGACTTT
    GTGTTGCTGA AAGAGCGGAC TCTGGACGAG GCCCCTCGGC CTCCCAAGAA GGCCATGGAC
    TACTCGTCGT CCAGCGAGGA GGTGGAAAGC AGTGAGGACG ACGAGGAGGA AGGCGAAGGC
    GGGCCAGCAG AGGGGAGCAG AGATACCCCT GGGGGCCGCA GCGATGGGGA TACAGACAGC
    GTCAGCACCA TGGTGGTCCA CGACGTCGAG GAGATCACCG GGACCCAGCC CCCATACGGG
    GGCGGCACCA TGGTGGTCCA GCGCACCCCT GAAGAGGAGC GGAACCTGCT GCATGCTGAC
    AGCAATGGGT ACACAAACCT GCCTGACGTG GTCCAGCCCA GCCACTCACC CACCGAGAAC
    AGCAAAGGCC AAAGCCCACC CTCGAAGGAT GGGAGTGGTG ACTACCAGTC TCGTGGGCTG
    GTAAAGGCCC CTGGCAAGAG CTCGTTCACG ATGTTTGTGG ATCTAGGGAT CTACCAGCCT
    GGAGGCAGTG GGGACAGCAT CCCCATCACA GCCCTAGTGG GTGGAGAGGG CACTCGGCTC
    GACCAGCTGC AGTACGACGT GAGGAAGGGT TCTGTGGTCA ACGTGAATCC CACCAACACC
    CGGGCCCACA GTGAGACCCC TGAGATCCGG AAGTACAAGA AGCGATTCAA CTCCGAGATC
    CTCTGTGCAG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAACGG GCTGATGTTG
    CTGGACCGAA GTGGGCAGGG CAAGGTGTAT GGACTCATTG GGCGGCGACG CTTCCAGCAG
    ATGGATGTGC TGGAGGGGCT CAACCTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG
    CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC AGAAGTGGAG
    AAGAAGCAGG GCTGGACCAC CGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGTGTTGTG
    AAATACGAGC GGATTAAGTT CCTGGTCATC GCCCTCAAGA GCTCCGTGGA GGTGTATGCC
    TGGGCCCCCA AACCCTACCA CAAATTCATG GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC
    CGCCCTCTGC TGGTCGACCT GACAGTAGAG GAGGGGCAGC GGCTCAAGGT CATCTATGGC
    TCCAGTGCTG GCTTCCATGC TGTGGATGTC GACTCGGGGA ACAGCTATGA CATCTACATC
    CCTGTGCACA TCCAGAGCCA GATCACGCCC CATGCCATCA TCTTCCTCCC CAACACCGAC
    GGCATGGAGA TGCTGCTGTG CTACGAGGAC GAGGGTGTCT ACGTCAACAC GTACGGGCGC
    ATCATTAAGG ATGTGGTGCT GCAGTGGGGG GAGATGCCTA CTTCTGTGGC CTACATCTGC
    TCCAACCAGA TAATGGGCTG GGGTGAGAAA GCCATTGAGA TCCGCTCTGT GGAGACGGGC
    CACCTCGACG GGGTCTTCAT GCACAAACGA GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG
    AATGACAAGG TGTTTTTTGC CTCAGTCCGC TCTGGGGGCA GCAGCCAAGT TTACTTCATG
    ACTCTGAACC GTAACTGCAT CATGAACTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016880197.1
    CDS109..3981
    Translation

    Target ORF information:

    RefSeq Version XM_017024708.1
    Organism Homo sapiens(human)
    Definition Homo sapiens misshapen like kinase 1 (MINK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017024708.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTGAAAA AGTACTCTCA CCACCGCAAC ATCGCCACCT ACTACGGAGC CTTCATCAAG 
    AAGAGCCCCC CGGGAAACGA TGACCAGCTC TGGCTGGTGA TGGAGTTCTG TGGTGCTGGT
    TCAGTGACTG ACCTGGTAAA GAACACAAAA GGCAACGCCC TGAAGGAGGA CTGTATCGCC
    TATATCTGCA GGGAGATCCT CAGGGGTCTG GCCCATCTCC ATGCCCACAA GGTGATCCAT
    CGAGACATCA AGGGGCAGAA TGTGCTGCTG ACAGAGAATG CTGAGGTCAA GCTAGTGGAT
    TTTGGGGTGA GTGCTCAGCT GGACCGCACC GTGGGCAGAC GGAACACTTT CATTGGGACT
    CCCTACTGGA TGGCTCCAGA GGTCATCGCC TGTGATGAGA ACCCTGATGC CACCTATGAT
    TACAGGAGTG ATATTTGGTC TCTAGGAATC ACAGCCATCG AGATGGCAGA GGGAGCCCCC
    CCTCTGTGTG ACATGCACCC CATGCGAGCC CTCTTCCTCA TTCCTCGGAA CCCTCCGCCC
    AGGCTCAAGT CCAAGAAGTG GTCTAAGAAG TTCATTGACT TCATTGACAC ATGTCTCATC
    AAGACTTACC TGAGCCGCCC ACCCACGGAG CAGCTACTGA AGTTTCCCTT CATCCGGGAC
    CAGCCCACGG AGCGGCAGGT CCGCATCCAG CTTAAGGACC ACATTGACCG ATCCCGGAAG
    AAGCGGGGTG AGAAAGAGGA GACAGAATAT GAGTACAGCG GCAGCGAGGA GGAAGATGAC
    AGCCATGGAG AGGAAGGAGA GCCAAGCTCC ATCATGAACG TGCCTGGAGA GTCGACTCTA
    CGCCGGGAGT TTCTCCGGCT CCAGCAGGAA AATAAGAGCA ACTCAGAGGC TTTAAAACAG
    CAGCAGCAGC TGCAGCAGCA GCAGCAGCGA GACCCCGAGG CACACATCAA ACACCTGCTG
    CACCAGCGGC AGCGGCGCAT AGAGGAGCAG AAGGAGGAGC GGCGCCGCGT GGAGGAGCAA
    CAGCGGCGGG AGCGGGAGCA GCGGAAGCTG CAGGAGAAGG AGCAGCAGCG GCGGCTGGAG
    GACATGCAGG CTCTGCGGCG GGAGGAGGAG CGGCGGCAGG CGGAGCGCGA GCAGGAATAT
    ATTCGTCACA GGCTAGAGGA GGAGCAGCGA CAGCTCGAGA TCCTTCAGCA ACAGCTGCTC
    CAGGAACAGG CCCTGCTGCT GGAATACAAG CGGAAGCAGC TGGAGGAGCA GCGGCAGTCA
    GAACGTCTCC AGAGGCAGCT GCAGCAGGAG CATGCCTACC TCAAGTCCCT GCAGCAGCAG
    CAACAGCAGC AGCAGCTTCA GAAACAGCAG CAGCAGCAGC TCCTGCCTGG GGACAGGAAG
    CCCCTGTACC ATTATGGTCG GGGCATGAAT CCCGCTGACA AACCAGCCTG GGCCCGAGAG
    GTAGAAGAGA GAACAAGGAT GAACAAGCAG CAGAACTCTC CCTTGGCCAA GAGCAAGCCA
    GGCAGCACGG GGCCTGAGCC CCCCATCCCC CAGGCCTCCC CAGGGCCCCC AGGACCCCTT
    TCCCAGACTC CTCCTATGCA GAGGCCGGTG GAGCCCCAGG AGGGACCGCA CAAGAGCCTG
    GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG TACCCCGATC CCAGTCCCTG
    CAGGACCAGC CCACCCGAAA CCTGGCTGCC TTCCCAGCCT CCCATGACCC CGACCCTGCC
    ATCCCCGCAC CCACTGCCAC GCCCAGTGCC CGAGGAGCTG TCATCCGCCA GAATTCAGAC
    CCCACCTCTG AAGGACCTGG CCCCAGCCCG AATCCCCCAG CCTGGGTCCG CCCAGATAAC
    GAGGCCCCAC CCAAGGTGCC TCAGAGGACC TCATCTATCG CCACTGCCCT TAACACCAGT
    GGGGCCGGAG GGTCCCGGCC AGCCCAGGCA GTCCGTGCCA GACCTCGCAG CAACTCCGCC
    TGGCAAATCT ATCTGCAAAG GCGGGCAGAG CGGGGCACCC CAAAGCCTCC AGGGCCCCCT
    GCTCAGCCCC CTGGCCCGCC CAACGCCTCT AGTAACCCCG ACCTCAGGAG GAGCGACCCT
    GGCTGGGAAC GCTCGGACAG CGTCCTTCCA GCCTCTCACG GGCACCTCCC CCAGGCTGGC
    TCACTGGAGC GGAACCGCGT GGGAGTCTCC TCCAAACCGG ACAGCTCCCC TGTGCTCTCC
    CCTGGGAATA AAGCCAAGCC CGACGACCAC CGCTCACGGC CAGGCCGGCC CGCAGACTTT
    GTGTTGCTGA AAGAGCGGAC TCTGGACGAG GCCCCTCGGC CTCCCAAGAA GGCCATGGAC
    TACTCGTCGT CCAGCGAGGA GGTGGAAAGC AGTGAGGACG ACGAGGAGGA AGGCGAAGGC
    GGGCCAGCAG AGGGGAGCAG AGATACCCCT GGGGGCCGCA GCGATGGGGA TACAGACAGC
    GTCAGCACCA TGGTGGTCCA CGACGTCGAG GAGATCACCG GGACCCAGCC CCCATACGGG
    GGCGGCACCA TGGTGGTCCA GCGCACCCCT GAAGAGGAGC GGAACCTGCT GCATGCTGAC
    AGCAATGGGT ACACAAACCT GCCTGACGTG GTCCAGCCCA GCCACTCACC CACCGAGAAC
    AGCAAAGGCC AAAGCCCACC CTCGAAGGAT GGGAGTGGTG ACTACCAGTC TCGTGGGCTG
    GTAAAGGCCC CTGGCAAGAG CTCGTTCACG ATGTTTGTGG ATCTAGGGAT CTACCAGCCT
    GGAGGCAGTG GGGACAGCAT CCCCATCACA GCCCTAGTGG GTGGAGAGGG CACTCGGCTC
    GACCAGCTGC AGTACGACGT GAGGAAGGGT TCTGTGGTCA ACGTGAATCC CACCAACACC
    CGGGCCCACA GTGAGACCCC TGAGATCCGG AAGTACAAGA AGCGATTCAA CTCCGAGATC
    CTCTGTGCAG CCCTTTGGGG GGTCAACCTG CTGGTGGGCA CGGAGAACGG GCTGATGTTG
    CTGGACCGAA GTGGGCAGGG CAAGGTGTAT GGACTCATTG GGCGGCGACG CTTCCAGCAG
    ATGGATGTGC TGGAGGGGCT CAACCTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG
    CGGGTGTATT ACCTGTCCTG GCTCCGGAAC AAGATTCTGC ACAATGACCC AGAAGTGGAG
    AAGAAGCAGG GCTGGACCAC CGTGGGGGAC ATGGAGGGCT GCGGGCACTA CCGTGTTGTG
    AAATACGAGC GGATTAAGTT CCTGGTCATC GCCCTCAAGA GCTCCGTGGA GGTGTATGCC
    TGGGCCCCCA AACCCTACCA CAAATTCATG GCCTTCAAGT CCTTTGCCGA CCTCCCCCAC
    CGCCCTCTGC TGGTCGACCT GACAGTAGAG GAGGGGCAGC GGCTCAAGGT CATCTATGGC
    TCCAGTGCTG GCTTCCATGC TGTGGATGTC GACTCGGGGA ACAGCTATGA CATCTACATC
    CCTGTGCACA TCCAGAGCCA GATCACGCCC CATGCCATCA TCTTCCTCCC CAACACCGAC
    GGCATGGAGA TGCTGCTGTG CTACGAGGAC GAGGGTGTCT ACGTCAACAC GTACGGGCGC
    ATCATTAAGG ATGTGGTGCT GCAGTGGGGG GAGATGCCTA CTTCTGTGGC CTACATCTGC
    TCCAACCAGA TAATGGGCTG GGGTGAGAAA GCCATTGAGA TCCGCTCTGT GGAGACGGGC
    CACCTCGACG GGGTCTTCAT GCACAAACGA GCTCAGAGGC TCAAGTTCCT GTGTGAGCGG
    AATGACAAGG TGTTTTTTGC CTCAGTCCGC TCTGGGGGCA GCAGCCAAGT TTACTTCATG
    ACTCTGAACC GTAACTGCAT CATGAACTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.