Mink1 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Mink1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mink1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa10385 NM_001271136.1
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33322 XM_006246716.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33323 XM_006246717.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33324 XM_006246718.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33326 XM_006246720.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33327 XM_006246721.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33328 XM_006246722.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
ORa33329 XM_006246723.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa33330 XM_006246724.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
ORa33331 XM_006246725.3
Latest version!
Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa10385
    Clone ID Related Accession (Same CDS sequence) NM_001271136.1
    Accession Version NM_001271136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-21
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072138.1 and AC119116.4. On Sep 2, 2012 this sequence version replaced XM_001079459.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAAGCTA TAAGCGAGCG
    ATTGGTGAGG ATTTCGTGTT GCTCAAAGAG CGGACTCTGG ATGAGGCCCC TAAGCCTCCC
    AAGAAGGCCA TGGACTATTC CTCATCCAGT GAGGAGGTGG AGAGCAGTGA AGATGAGGAG
    GAGGAAGGCG ATGGGGAGCC ATCAGAGGGG AGCAGAGACA CTCCCGGGGG CCGTGATGGT
    GACACAGATA GCGTCAGCAC CATGGTGGTT CATGATGTTG AAGAGGTATC CGGGACCCAG
    CCTTCATATG GAGGAGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA GCGAAGCCTG
    CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCAGATG TGGTCCAGCC CAGCCACTCA
    CCTACTGAGA ACAGCCAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCGG TGATTACCAG
    TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGTTCATTCA CCATGTTTGT GGATCTAGGG
    ATCTACCAGC CTGGAGGCAG TGGGGATACC ATCCCCATCA CAGCCCTAGT GGGCGGAGAG
    GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT
    CCCACCAACA CTCGAGCTCA TAGTGAAACT CCCGAGATTC GCAAGTACAA GAAGCGATTC
    AATTCCGAGA TCCTCTGTGC AGCCCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT
    GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAAGTGT ATGGACTTAT TGGGCGACGA
    CGCTTCCAGC AAATGGATGT TCTGGAAGGG CTCAACCTGC TCATCACCAT CTCAGGGAAA
    AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ATAAGATTCT ACACAATGAC
    CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC
    TATCGTGTTG TGAAATATGA ACGAATTAAG TTCCTGGTCA TTGCTCTGAA GAACTCTGTG
    GAGGTGTATG CCTGGGCCCC TAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT
    GACCTCCCTC ACCGCCCTCT GCTGGTGGAC CTGACAGTAG AGGAGGGCCA GCGGCTCAAG
    GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGACTCTGG GAACAGCTAT
    GACATCTACA TCCCTGTACA TATCCAGAGC CAAATCACAC CCCACGCCAT CGTCTTTCTC
    CCCAACACTG ACGGCATGGA GATGCTGCTT TGCTATGAAG ATGAGGGTGT CTACGTCAAC
    ACTTACGGGC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTC
    GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT
    GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC
    CTGTGTGAGC GGAATGATAA GGTGTTTTTT GCCTCAGTCC GCTCTGGAGG AAGCAGCCAA
    GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258065.1
    CDS188..4252
    Translation

    Target ORF information:

    RefSeq Version NM_001271136.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAAGCTA TAAGCGAGCG
    ATTGGTGAGG ATTTCGTGTT GCTCAAAGAG CGGACTCTGG ATGAGGCCCC TAAGCCTCCC
    AAGAAGGCCA TGGACTATTC CTCATCCAGT GAGGAGGTGG AGAGCAGTGA AGATGAGGAG
    GAGGAAGGCG ATGGGGAGCC ATCAGAGGGG AGCAGAGACA CTCCCGGGGG CCGTGATGGT
    GACACAGATA GCGTCAGCAC CATGGTGGTT CATGATGTTG AAGAGGTATC CGGGACCCAG
    CCTTCATATG GAGGAGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA GCGAAGCCTG
    CTGCTTGCTG ATAGCAATGG CTACACAAAC CTGCCAGATG TGGTCCAGCC CAGCCACTCA
    CCTACTGAGA ACAGCCAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCGG TGATTACCAG
    TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG AGTTCATTCA CCATGTTTGT GGATCTAGGG
    ATCTACCAGC CTGGAGGCAG TGGGGATACC ATCCCCATCA CAGCCCTAGT GGGCGGAGAG
    GGTGGTCGCC TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT
    CCCACCAACA CTCGAGCTCA TAGTGAAACT CCCGAGATTC GCAAGTACAA GAAGCGATTC
    AATTCCGAGA TCCTCTGTGC AGCCCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT
    GGGCTGATGT TGCTGGACCG AAGTGGGCAG GGCAAAGTGT ATGGACTTAT TGGGCGACGA
    CGCTTCCAGC AAATGGATGT TCTGGAAGGG CTCAACCTGC TCATCACCAT CTCAGGGAAA
    AGGAACAAAC TGCGGGTATA TTACCTGTCC TGGCTTCGGA ATAAGATTCT ACACAATGAC
    CCAGAGGTGG AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC
    TATCGTGTTG TGAAATATGA ACGAATTAAG TTCCTGGTCA TTGCTCTGAA GAACTCTGTG
    GAGGTGTATG CCTGGGCCCC TAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT
    GACCTCCCTC ACCGCCCTCT GCTGGTGGAC CTGACAGTAG AGGAGGGCCA GCGGCTCAAG
    GTCATCTATG GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGACTCTGG GAACAGCTAT
    GACATCTACA TCCCTGTACA TATCCAGAGC CAAATCACAC CCCACGCCAT CGTCTTTCTC
    CCCAACACTG ACGGCATGGA GATGCTGCTT TGCTATGAAG ATGAGGGTGT CTACGTCAAC
    ACTTACGGGC GGATCATCAA GGACGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTC
    GCCTACATCT GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT
    GTGGAGACAG GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC
    CTGTGTGAGC GGAATGATAA GGTGTTTTTT GCCTCAGTCC GCTCTGGAGG AAGCAGCCAA
    GTTTACTTTA TGACTCTGAA CCGTAACTGC ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33322
    Clone ID Related Accession (Same CDS sequence) XM_006246716.3
    Accession Version XM_006246716.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246716.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAAGCTATAA GCGAGCGATT GGTGAGGATT TCGTGTTGCT CAAAGAGCGG
    ACTCTGGATG AGGCCCCTAA GCCTCCCAAG AAGGCCATGG ACTATTCCTC ATCCAGTGAG
    GAGGTGGAGA GCAGTGAAGA TGAGGAGGAG GAAGGCGATG GGGAGCCATC AGAGGGGAGC
    AGAGACACTC CCGGGGGCCG CAGTGATGGT GACACAGATA GCGTCAGCAC CATGGTGGTT
    CATGATGTTG AAGAGGTATC CGGGACCCAG CCTTCATATG GAGGAGGCAC CATGGTGGTC
    CAGCGTACTC CTGAAGAGGA GCGAAGCCTG CTGCTTGCTG ATAGCAATGG CTACACAAAC
    CTGCCAGATG TGGTCCAGCC CAGCCACTCA CCTACTGAGA ACAGCCAAGG TCAAAGCCCT
    CCAACAAAGG ATGGAGGCGG TGATTACCAG TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG
    AGTTCATTCA CCATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGATACC
    ATCCCCATCA CAGCCCTAGT GGGCGGAGAG GGTGGTCGCC TTGATCAACT GCAGTTCGAT
    GTGAGGAAGG GCTCTGTGGT CAACGTCAAT CCCACCAACA CTCGAGCTCA TAGTGAAACT
    CCCGAGATTC GCAAGTACAA GAAGCGATTC AATTCCGAGA TCCTCTGTGC AGCCCTCTGG
    GGGGTCAACC TCCTAGTGGG CACAGAGAAT GGGCTGATGT TGCTGGACCG AAGTGGGCAG
    GGCAAAGTGT ATGGACTTAT TGGGCGACGA CGCTTCCAGC AAATGGATGT TCTGGAAGGG
    CTCAACCTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTATA TTACCTGTCC
    TGGCTTCGGA ATAAGATTCT ACACAATGAC CCAGAGGTGG AAAAGAAGCA GGGGTGGACC
    ACCGTGGGGG ACATGGAGGG CTGCGGCCAC TATCGTGTTG TGAAATATGA ACGAATTAAG
    TTCCTGGTCA TTGCTCTGAA GAACTCTGTG GAGGTGTATG CCTGGGCCCC TAAACCCTAC
    CACAAATTCA TGGCCTTCAA GTCCTTTGCT GACCTCCCTC ACCGCCCTCT GCTGGTGGAC
    CTGACAGTAG AGGAGGGCCA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT
    GCTGTGGATG TTGACTCTGG GAACAGCTAT GACATCTACA TCCCTGTACA TATCCAGAGC
    CAAATCACAC CCCACGCCAT CGTCTTTCTC CCCAACACTG ACGGCATGGA GATGCTGCTT
    TGCTATGAAG ATGAGGGTGT CTACGTCAAC ACTTACGGGC GGATCATCAA GGACGTGGTG
    CTGCAGTGGG GAGAGATGCC CACCTCTGTC GCCTACATCT GCTCCAACCA GATAATGGGC
    TGGGGTGAGA AGGCCATAGA GATCCGCTCT GTGGAGACAG GCCACCTAGA TGGGGTCTTC
    ATGCACAAAC GAGCCCAGAG GCTCAAGTTC CTGTGTGAGC GGAATGATAA GGTGTTTTTT
    GCCTCAGTCC GCTCTGGAGG AAGCAGCCAA GTTTACTTTA TGACTCTGAA CCGTAACTGC
    ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246778.1
    CDS225..4379
    Translation

    Target ORF information:

    RefSeq Version XM_006246716.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246716.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAAGCTATAA GCGAGCGATT GGTGAGGATT TCGTGTTGCT CAAAGAGCGG
    ACTCTGGATG AGGCCCCTAA GCCTCCCAAG AAGGCCATGG ACTATTCCTC ATCCAGTGAG
    GAGGTGGAGA GCAGTGAAGA TGAGGAGGAG GAAGGCGATG GGGAGCCATC AGAGGGGAGC
    AGAGACACTC CCGGGGGCCG CAGTGATGGT GACACAGATA GCGTCAGCAC CATGGTGGTT
    CATGATGTTG AAGAGGTATC CGGGACCCAG CCTTCATATG GAGGAGGCAC CATGGTGGTC
    CAGCGTACTC CTGAAGAGGA GCGAAGCCTG CTGCTTGCTG ATAGCAATGG CTACACAAAC
    CTGCCAGATG TGGTCCAGCC CAGCCACTCA CCTACTGAGA ACAGCCAAGG TCAAAGCCCT
    CCAACAAAGG ATGGAGGCGG TGATTACCAG TCTCGTGGGC TGGTAAAGGC CCCAGGAAAG
    AGTTCATTCA CCATGTTTGT GGATCTAGGG ATCTACCAGC CTGGAGGCAG TGGGGATACC
    ATCCCCATCA CAGCCCTAGT GGGCGGAGAG GGTGGTCGCC TTGATCAACT GCAGTTCGAT
    GTGAGGAAGG GCTCTGTGGT CAACGTCAAT CCCACCAACA CTCGAGCTCA TAGTGAAACT
    CCCGAGATTC GCAAGTACAA GAAGCGATTC AATTCCGAGA TCCTCTGTGC AGCCCTCTGG
    GGGGTCAACC TCCTAGTGGG CACAGAGAAT GGGCTGATGT TGCTGGACCG AAGTGGGCAG
    GGCAAAGTGT ATGGACTTAT TGGGCGACGA CGCTTCCAGC AAATGGATGT TCTGGAAGGG
    CTCAACCTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC TGCGGGTATA TTACCTGTCC
    TGGCTTCGGA ATAAGATTCT ACACAATGAC CCAGAGGTGG AAAAGAAGCA GGGGTGGACC
    ACCGTGGGGG ACATGGAGGG CTGCGGCCAC TATCGTGTTG TGAAATATGA ACGAATTAAG
    TTCCTGGTCA TTGCTCTGAA GAACTCTGTG GAGGTGTATG CCTGGGCCCC TAAACCCTAC
    CACAAATTCA TGGCCTTCAA GTCCTTTGCT GACCTCCCTC ACCGCCCTCT GCTGGTGGAC
    CTGACAGTAG AGGAGGGCCA GCGGCTCAAG GTCATCTATG GCTCCAGTGC TGGCTTCCAT
    GCTGTGGATG TTGACTCTGG GAACAGCTAT GACATCTACA TCCCTGTACA TATCCAGAGC
    CAAATCACAC CCCACGCCAT CGTCTTTCTC CCCAACACTG ACGGCATGGA GATGCTGCTT
    TGCTATGAAG ATGAGGGTGT CTACGTCAAC ACTTACGGGC GGATCATCAA GGACGTGGTG
    CTGCAGTGGG GAGAGATGCC CACCTCTGTC GCCTACATCT GCTCCAACCA GATAATGGGC
    TGGGGTGAGA AGGCCATAGA GATCCGCTCT GTGGAGACAG GCCACCTAGA TGGGGTCTTC
    ATGCACAAAC GAGCCCAGAG GCTCAAGTTC CTGTGTGAGC GGAATGATAA GGTGTTTTTT
    GCCTCAGTCC GCTCTGGAGG AAGCAGCCAA GTTTACTTTA TGACTCTGAA CCGTAACTGC
    ATCATGAACT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33323
    Clone ID Related Accession (Same CDS sequence) XM_006246717.3
    Accession Version XM_006246717.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246717.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAAGCTATAA GCGAGCGATT GGTGAGGATT TCGTGTTGCT CAAAGAGCGG
    ACTCTGGATG AGGCCCCTAA GCCTCCCAAG AAGGCCATGG ACTATTCCTC ATCCAGTGAG
    GAGGTGGAGA GCAGTGAAGA TGAGGAGGAG GAAGGCGATG GGGAGCCATC AGAGGGGAGC
    AGAGACACTC CCGGGGGCCG TGATGGTGAC ACAGATAGCG TCAGCACCAT GGTGGTTCAT
    GATGTTGAAG AGGTATCCGG GACCCAGCCT TCATATGGAG GAGGCACCAT GGTGGTCCAG
    CGTACTCCTG AAGAGGAGCG AAGCCTGCTG CTTGCTGATA GCAATGGCTA CACAAACCTG
    CCAGATGTGG TCCAGCCCAG CCACTCACCT ACTGAGAACA GCCAAGGTCA AAGCCCTCCA
    ACAAAGGATG GAGGCGGTGA TTACCAGTCT CGTGGGCTGG TAAAGGCCCC AGGAAAGAGT
    TCATTCACCA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGATACCATC
    CCCATCACAG CCCTAGTGGG CGGAGAGGGT GGTCGCCTTG ATCAACTGCA GTTCGATGTG
    AGGAAGGGCT CTGTGGTCAA CGTCAATCCC ACCAACACTC GAGCTCATAG TGAAACTCCC
    GAGATTCGCA AGTACAAGAA GCGATTCAAT TCCGAGATCC TCTGTGCAGC CCTCTGGGGG
    GTCAACCTCC TAGTGGGCAC AGAGAATGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAAGTGTATG GACTTATTGG GCGACGACGC TTCCAGCAAA TGGATGTTCT GGAAGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTATATTA CCTGTCCTGG
    CTTCGGAATA AGATTCTACA CAATGACCCA GAGGTGGAAA AGAAGCAGGG GTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGCCACTAT CGTGTTGTGA AATATGAACG AATTAAGTTC
    CTGGTCATTG CTCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCTAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCTGAC CTCCCTCACC GCCCTCTGCT GGTGGACCTG
    ACAGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTTG ACTCTGGGAA CAGCTATGAC ATCTACATCC CTGTACATAT CCAGAGCCAA
    ATCACACCCC ACGCCATCGT CTTTCTCCCC AACACTGACG GCATGGAGAT GCTGCTTTGC
    TATGAAGATG AGGGTGTCTA CGTCAACACT TACGGGCGGA TCATCAAGGA CGTGGTGCTG
    CAGTGGGGAG AGATGCCCAC CTCTGTCGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAGG CCATAGAGAT CCGCTCTGTG GAGACAGGCC ACCTAGATGG GGTCTTCATG
    CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGATAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGAGGAAG CAGCCAAGTT TACTTTATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246779.1
    CDS225..4376
    Translation

    Target ORF information:

    RefSeq Version XM_006246717.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246717.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAAGCTATAA GCGAGCGATT GGTGAGGATT TCGTGTTGCT CAAAGAGCGG
    ACTCTGGATG AGGCCCCTAA GCCTCCCAAG AAGGCCATGG ACTATTCCTC ATCCAGTGAG
    GAGGTGGAGA GCAGTGAAGA TGAGGAGGAG GAAGGCGATG GGGAGCCATC AGAGGGGAGC
    AGAGACACTC CCGGGGGCCG TGATGGTGAC ACAGATAGCG TCAGCACCAT GGTGGTTCAT
    GATGTTGAAG AGGTATCCGG GACCCAGCCT TCATATGGAG GAGGCACCAT GGTGGTCCAG
    CGTACTCCTG AAGAGGAGCG AAGCCTGCTG CTTGCTGATA GCAATGGCTA CACAAACCTG
    CCAGATGTGG TCCAGCCCAG CCACTCACCT ACTGAGAACA GCCAAGGTCA AAGCCCTCCA
    ACAAAGGATG GAGGCGGTGA TTACCAGTCT CGTGGGCTGG TAAAGGCCCC AGGAAAGAGT
    TCATTCACCA TGTTTGTGGA TCTAGGGATC TACCAGCCTG GAGGCAGTGG GGATACCATC
    CCCATCACAG CCCTAGTGGG CGGAGAGGGT GGTCGCCTTG ATCAACTGCA GTTCGATGTG
    AGGAAGGGCT CTGTGGTCAA CGTCAATCCC ACCAACACTC GAGCTCATAG TGAAACTCCC
    GAGATTCGCA AGTACAAGAA GCGATTCAAT TCCGAGATCC TCTGTGCAGC CCTCTGGGGG
    GTCAACCTCC TAGTGGGCAC AGAGAATGGG CTGATGTTGC TGGACCGAAG TGGGCAGGGC
    AAAGTGTATG GACTTATTGG GCGACGACGC TTCCAGCAAA TGGATGTTCT GGAAGGGCTC
    AACCTGCTCA TCACCATCTC AGGGAAAAGG AACAAACTGC GGGTATATTA CCTGTCCTGG
    CTTCGGAATA AGATTCTACA CAATGACCCA GAGGTGGAAA AGAAGCAGGG GTGGACCACC
    GTGGGGGACA TGGAGGGCTG CGGCCACTAT CGTGTTGTGA AATATGAACG AATTAAGTTC
    CTGGTCATTG CTCTGAAGAA CTCTGTGGAG GTGTATGCCT GGGCCCCTAA ACCCTACCAC
    AAATTCATGG CCTTCAAGTC CTTTGCTGAC CTCCCTCACC GCCCTCTGCT GGTGGACCTG
    ACAGTAGAGG AGGGCCAGCG GCTCAAGGTC ATCTATGGCT CCAGTGCTGG CTTCCATGCT
    GTGGATGTTG ACTCTGGGAA CAGCTATGAC ATCTACATCC CTGTACATAT CCAGAGCCAA
    ATCACACCCC ACGCCATCGT CTTTCTCCCC AACACTGACG GCATGGAGAT GCTGCTTTGC
    TATGAAGATG AGGGTGTCTA CGTCAACACT TACGGGCGGA TCATCAAGGA CGTGGTGCTG
    CAGTGGGGAG AGATGCCCAC CTCTGTCGCC TACATCTGCT CCAACCAGAT AATGGGCTGG
    GGTGAGAAGG CCATAGAGAT CCGCTCTGTG GAGACAGGCC ACCTAGATGG GGTCTTCATG
    CACAAACGAG CCCAGAGGCT CAAGTTCCTG TGTGAGCGGA ATGATAAGGT GTTTTTTGCC
    TCAGTCCGCT CTGGAGGAAG CAGCCAAGTT TACTTTATGA CTCTGAACCG TAACTGCATC
    ATGAACTGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33324
    Clone ID Related Accession (Same CDS sequence) XM_006246718.3
    Accession Version XM_006246718.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246718.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAGATTTCGT GTTGCTCAAA GAGCGGACTC TGGATGAGGC CCCTAAGCCT
    CCCAAGAAGG CCATGGACTA TTCCTCATCC AGTGAGGAGG TGGAGAGCAG TGAAGATGAG
    GAGGAGGAAG GCGATGGGGA GCCATCAGAG GGGAGCAGAG ACACTCCCGG GGGCCGCAGT
    GATGGTGACA CAGATAGCGT CAGCACCATG GTGGTTCATG ATGTTGAAGA GGTATCCGGG
    ACCCAGCCTT CATATGGAGG AGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAGCGA
    AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CAGATGTGGT CCAGCCCAGC
    CACTCACCTA CTGAGAACAG CCAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCGGTGAT
    TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGTT CATTCACCAT GTTTGTGGAT
    CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GATACCATCC CCATCACAGC CCTAGTGGGC
    GGAGAGGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC
    GTCAATCCCA CCAACACTCG AGCTCATAGT GAAACTCCCG AGATTCGCAA GTACAAGAAG
    CGATTCAATT CCGAGATCCT CTGTGCAGCC CTCTGGGGGG TCAACCTCCT AGTGGGCACA
    GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AAGTGTATGG ACTTATTGGG
    CGACGACGCT TCCAGCAAAT GGATGTTCTG GAAGGGCTCA ACCTGCTCAT CACCATCTCA
    GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAATAA GATTCTACAC
    AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC
    GGCCACTATC GTGTTGTGAA ATATGAACGA ATTAAGTTCC TGGTCATTGC TCTGAAGAAC
    TCTGTGGAGG TGTATGCCTG GGCCCCTAAA CCCTACCACA AATTCATGGC CTTCAAGTCC
    TTTGCTGACC TCCCTCACCG CCCTCTGCTG GTGGACCTGA CAGTAGAGGA GGGCCAGCGG
    CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA CTCTGGGAAC
    AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAAA TCACACCCCA CGCCATCGTC
    TTTCTCCCCA ACACTGACGG CATGGAGATG CTGCTTTGCT ATGAAGATGA GGGTGTCTAC
    GTCAACACTT ACGGGCGGAT CATCAAGGAC GTGGTGCTGC AGTGGGGAGA GATGCCCACC
    TCTGTCGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC
    CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC
    AAGTTCCTGT GTGAGCGGAA TGATAAGGTG TTTTTTGCCT CAGTCCGCTC TGGAGGAAGC
    AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246780.1
    CDS225..4355
    Translation

    Target ORF information:

    RefSeq Version XM_006246718.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246718.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGACCTCGC
    AGCAACTCCG CCTGGCAAAT CTATCTGCAG AGGCGGGCAG AGCGAGGCAC CCCCAAGCCT
    CCTGGGCCCC CAGCTCAGCC CCCTGGCCCG CCCAACACCT CTAGTAACCC TGACCTCAGG
    AGGAGTGACC CTGGCTGGGA GCGTTCAGAC AGTGTCCTCC CTGCCTCCCA CGGCCACCTC
    CCTCAGGCCG GCTCCTTGGA GCGGAACCGA AACCGTGTGG GAGCCTCCAC AAAACTGGAC
    AGCTCCCCAG TGCTCTCCCC TGGGAACAAG GCCAAGCCTG AAGACCACCG CTCAAGGCCA
    GGCCGGCCCG CAGATTTCGT GTTGCTCAAA GAGCGGACTC TGGATGAGGC CCCTAAGCCT
    CCCAAGAAGG CCATGGACTA TTCCTCATCC AGTGAGGAGG TGGAGAGCAG TGAAGATGAG
    GAGGAGGAAG GCGATGGGGA GCCATCAGAG GGGAGCAGAG ACACTCCCGG GGGCCGCAGT
    GATGGTGACA CAGATAGCGT CAGCACCATG GTGGTTCATG ATGTTGAAGA GGTATCCGGG
    ACCCAGCCTT CATATGGAGG AGGCACCATG GTGGTCCAGC GTACTCCTGA AGAGGAGCGA
    AGCCTGCTGC TTGCTGATAG CAATGGCTAC ACAAACCTGC CAGATGTGGT CCAGCCCAGC
    CACTCACCTA CTGAGAACAG CCAAGGTCAA AGCCCTCCAA CAAAGGATGG AGGCGGTGAT
    TACCAGTCTC GTGGGCTGGT AAAGGCCCCA GGAAAGAGTT CATTCACCAT GTTTGTGGAT
    CTAGGGATCT ACCAGCCTGG AGGCAGTGGG GATACCATCC CCATCACAGC CCTAGTGGGC
    GGAGAGGGTG GTCGCCTTGA TCAACTGCAG TTCGATGTGA GGAAGGGCTC TGTGGTCAAC
    GTCAATCCCA CCAACACTCG AGCTCATAGT GAAACTCCCG AGATTCGCAA GTACAAGAAG
    CGATTCAATT CCGAGATCCT CTGTGCAGCC CTCTGGGGGG TCAACCTCCT AGTGGGCACA
    GAGAATGGGC TGATGTTGCT GGACCGAAGT GGGCAGGGCA AAGTGTATGG ACTTATTGGG
    CGACGACGCT TCCAGCAAAT GGATGTTCTG GAAGGGCTCA ACCTGCTCAT CACCATCTCA
    GGGAAAAGGA ACAAACTGCG GGTATATTAC CTGTCCTGGC TTCGGAATAA GATTCTACAC
    AATGACCCAG AGGTGGAAAA GAAGCAGGGG TGGACCACCG TGGGGGACAT GGAGGGCTGC
    GGCCACTATC GTGTTGTGAA ATATGAACGA ATTAAGTTCC TGGTCATTGC TCTGAAGAAC
    TCTGTGGAGG TGTATGCCTG GGCCCCTAAA CCCTACCACA AATTCATGGC CTTCAAGTCC
    TTTGCTGACC TCCCTCACCG CCCTCTGCTG GTGGACCTGA CAGTAGAGGA GGGCCAGCGG
    CTCAAGGTCA TCTATGGCTC CAGTGCTGGC TTCCATGCTG TGGATGTTGA CTCTGGGAAC
    AGCTATGACA TCTACATCCC TGTACATATC CAGAGCCAAA TCACACCCCA CGCCATCGTC
    TTTCTCCCCA ACACTGACGG CATGGAGATG CTGCTTTGCT ATGAAGATGA GGGTGTCTAC
    GTCAACACTT ACGGGCGGAT CATCAAGGAC GTGGTGCTGC AGTGGGGAGA GATGCCCACC
    TCTGTCGCCT ACATCTGCTC CAACCAGATA ATGGGCTGGG GTGAGAAGGC CATAGAGATC
    CGCTCTGTGG AGACAGGCCA CCTAGATGGG GTCTTCATGC ACAAACGAGC CCAGAGGCTC
    AAGTTCCTGT GTGAGCGGAA TGATAAGGTG TTTTTTGCCT CAGTCCGCTC TGGAGGAAGC
    AGCCAAGTTT ACTTTATGAC TCTGAACCGT AACTGCATCA TGAACTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33326
    Clone ID Related Accession (Same CDS sequence) XM_006246720.3
    Accession Version XM_006246720.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4068bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAAGCTA TAAGCGAGCG
    ATTGGTGAGG ATTTCGTGTT GCTCAAAGAG CGGACTCTGG ATGAGGCCCC TAAGCCTCCC
    AAGAAGGCCA TGGACTATTC CTCATCCAGT GAGGAGGTGG AGAGCAGTGA AGATGAGGAG
    GAGGAAGGCG ATGGGGAGCC ATCAGAGGGG AGCAGAGACA CTCCCGGGGG CCGCAGTGAT
    GGTGACACAG ATAGCGTCAG CACCATGGTG GTTCATGATG TTGAAGAGGT ATCCGGGACC
    CAGCCTTCAT ATGGAGGAGG CACCATGGTG GTCCAGCGTA CTCCTGAAGA GGAGCGAAGC
    CTGCTGCTTG CTGATAGCAA TGGCTACACA AACCTGCCAG ATGTGGTCCA GCCCAGCCAC
    TCACCTACTG AGAACAGCCA AGGTCAAAGC CCTCCAACAA AGGATGGAGG CGGTGATTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA AAGAGTTCAT TCACCATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAT ACCATCCCCA TCACAGCCCT AGTGGGCGGA
    GAGGGTGGTC GCCTTGATCA ACTGCAGTTC GATGTGAGGA AGGGCTCTGT GGTCAACGTC
    AATCCCACCA ACACTCGAGC TCATAGTGAA ACTCCCGAGA TTCGCAAGTA CAAGAAGCGA
    TTCAATTCCG AGATCCTCTG TGCAGCCCTC TGGGGGGTCA ACCTCCTAGT GGGCACAGAG
    AATGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAAG TGTATGGACT TATTGGGCGA
    CGACGCTTCC AGCAAATGGA TGTTCTGGAA GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT ATATTACCTG TCCTGGCTTC GGAATAAGAT TCTACACAAT
    GACCCAGAGG TGGAAAAGAA GCAGGGGTGG ACCACCGTGG GGGACATGGA GGGCTGCGGC
    CACTATCGTG TTGTGAAATA TGAACGAATT AAGTTCCTGG TCATTGCTCT GAAGAACTCT
    GTGGAGGTGT ATGCCTGGGC CCCTAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCTGACCTCC CTCACCGCCC TCTGCTGGTG GACCTGACAG TAGAGGAGGG CCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTTGACTC TGGGAACAGC
    TATGACATCT ACATCCCTGT ACATATCCAG AGCCAAATCA CACCCCACGC CATCGTCTTT
    CTCCCCAACA CTGACGGCAT GGAGATGCTG CTTTGCTATG AAGATGAGGG TGTCTACGTC
    AACACTTACG GGCGGATCAT CAAGGACGTG GTGCTGCAGT GGGGAGAGAT GCCCACCTCT
    GTCGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAGGCCAT AGAGATCCGC
    TCTGTGGAGA CAGGCCACCT AGATGGGGTC TTCATGCACA AACGAGCCCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA TAAGGTGTTT TTTGCCTCAG TCCGCTCTGG AGGAAGCAGC
    CAAGTTTACT TTATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246782.1
    CDS225..4292
    Translation

    Target ORF information:

    RefSeq Version XM_006246720.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246720.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAAGCTA TAAGCGAGCG
    ATTGGTGAGG ATTTCGTGTT GCTCAAAGAG CGGACTCTGG ATGAGGCCCC TAAGCCTCCC
    AAGAAGGCCA TGGACTATTC CTCATCCAGT GAGGAGGTGG AGAGCAGTGA AGATGAGGAG
    GAGGAAGGCG ATGGGGAGCC ATCAGAGGGG AGCAGAGACA CTCCCGGGGG CCGCAGTGAT
    GGTGACACAG ATAGCGTCAG CACCATGGTG GTTCATGATG TTGAAGAGGT ATCCGGGACC
    CAGCCTTCAT ATGGAGGAGG CACCATGGTG GTCCAGCGTA CTCCTGAAGA GGAGCGAAGC
    CTGCTGCTTG CTGATAGCAA TGGCTACACA AACCTGCCAG ATGTGGTCCA GCCCAGCCAC
    TCACCTACTG AGAACAGCCA AGGTCAAAGC CCTCCAACAA AGGATGGAGG CGGTGATTAC
    CAGTCTCGTG GGCTGGTAAA GGCCCCAGGA AAGAGTTCAT TCACCATGTT TGTGGATCTA
    GGGATCTACC AGCCTGGAGG CAGTGGGGAT ACCATCCCCA TCACAGCCCT AGTGGGCGGA
    GAGGGTGGTC GCCTTGATCA ACTGCAGTTC GATGTGAGGA AGGGCTCTGT GGTCAACGTC
    AATCCCACCA ACACTCGAGC TCATAGTGAA ACTCCCGAGA TTCGCAAGTA CAAGAAGCGA
    TTCAATTCCG AGATCCTCTG TGCAGCCCTC TGGGGGGTCA ACCTCCTAGT GGGCACAGAG
    AATGGGCTGA TGTTGCTGGA CCGAAGTGGG CAGGGCAAAG TGTATGGACT TATTGGGCGA
    CGACGCTTCC AGCAAATGGA TGTTCTGGAA GGGCTCAACC TGCTCATCAC CATCTCAGGG
    AAAAGGAACA AACTGCGGGT ATATTACCTG TCCTGGCTTC GGAATAAGAT TCTACACAAT
    GACCCAGAGG TGGAAAAGAA GCAGGGGTGG ACCACCGTGG GGGACATGGA GGGCTGCGGC
    CACTATCGTG TTGTGAAATA TGAACGAATT AAGTTCCTGG TCATTGCTCT GAAGAACTCT
    GTGGAGGTGT ATGCCTGGGC CCCTAAACCC TACCACAAAT TCATGGCCTT CAAGTCCTTT
    GCTGACCTCC CTCACCGCCC TCTGCTGGTG GACCTGACAG TAGAGGAGGG CCAGCGGCTC
    AAGGTCATCT ATGGCTCCAG TGCTGGCTTC CATGCTGTGG ATGTTGACTC TGGGAACAGC
    TATGACATCT ACATCCCTGT ACATATCCAG AGCCAAATCA CACCCCACGC CATCGTCTTT
    CTCCCCAACA CTGACGGCAT GGAGATGCTG CTTTGCTATG AAGATGAGGG TGTCTACGTC
    AACACTTACG GGCGGATCAT CAAGGACGTG GTGCTGCAGT GGGGAGAGAT GCCCACCTCT
    GTCGCCTACA TCTGCTCCAA CCAGATAATG GGCTGGGGTG AGAAGGCCAT AGAGATCCGC
    TCTGTGGAGA CAGGCCACCT AGATGGGGTC TTCATGCACA AACGAGCCCA GAGGCTCAAG
    TTCCTGTGTG AGCGGAATGA TAAGGTGTTT TTTGCCTCAG TCCGCTCTGG AGGAAGCAGC
    CAAGTTTACT TTATGACTCT GAACCGTAAC TGCATCATGA ACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33327
    Clone ID Related Accession (Same CDS sequence) XM_006246721.3
    Accession Version XM_006246721.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGTAACCCT
    GACCTCAGGA GGAGTGACCC TGGCTGGGAG CGTTCAGACA GTGTCCTCCC TGCCTCCCAC
    GGCCACCTCC CTCAGGCCGG CTCCTTGGAG CGGAACCGAA ACCGTGTGGG AGCCTCCACA
    AAACTGGACA GCTCCCCAGT GCTCTCCCCT GGGAACAAGG CCAAGCCTGA AGACCACCGC
    TCAAGGCCAG GCCGGCCCGC AAGCTATAAG CGAGCGATTG GTGAGGATTT CGTGTTGCTC
    AAAGAGCGGA CTCTGGATGA GGCCCCTAAG CCTCCCAAGA AGGCCATGGA CTATTCCTCA
    TCCAGTGAGG AGGTGGAGAG CAGTGAAGAT GAGGAGGAGG AAGGCGATGG GGAGCCATCA
    GAGGGGAGCA GAGACACTCC CGGGGGCCGC AGTGATGGTG ACACAGATAG CGTCAGCACC
    ATGGTGGTTC ATGATGTTGA AGAGGTATCC GGGACCCAGC CTTCATATGG AGGAGGCACC
    ATGGTGGTCC AGCGTACTCC TGAAGAGGAG CGAAGCCTGC TGCTTGCTGA TAGCAATGGC
    TACACAAACC TGCCAGATGT GGTCCAGCCC AGCCACTCAC CTACTGAGAA CAGCCAAGGT
    CAAAGCCCTC CAACAAAGGA TGGAGGCGGT GATTACCAGT CTCGTGGGCT GGTAAAGGCC
    CCAGGAAAGA GTTCATTCAC CATGTTTGTG GATCTAGGGA TCTACCAGCC TGGAGGCAGT
    GGGGATACCA TCCCCATCAC AGCCCTAGTG GGCGGAGAGG GTGGTCGCCT TGATCAACTG
    CAGTTCGATG TGAGGAAGGG CTCTGTGGTC AACGTCAATC CCACCAACAC TCGAGCTCAT
    AGTGAAACTC CCGAGATTCG CAAGTACAAG AAGCGATTCA ATTCCGAGAT CCTCTGTGCA
    GCCCTCTGGG GGGTCAACCT CCTAGTGGGC ACAGAGAATG GGCTGATGTT GCTGGACCGA
    AGTGGGCAGG GCAAAGTGTA TGGACTTATT GGGCGACGAC GCTTCCAGCA AATGGATGTT
    CTGGAAGGGC TCAACCTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTATAT
    TACCTGTCCT GGCTTCGGAA TAAGATTCTA CACAATGACC CAGAGGTGGA AAAGAAGCAG
    GGGTGGACCA CCGTGGGGGA CATGGAGGGC TGCGGCCACT ATCGTGTTGT GAAATATGAA
    CGAATTAAGT TCCTGGTCAT TGCTCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCT
    AAACCCTACC ACAAATTCAT GGCCTTCAAG TCCTTTGCTG ACCTCCCTCA CCGCCCTCTG
    CTGGTGGACC TGACAGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGTGCT
    GGCTTCCATG CTGTGGATGT TGACTCTGGG AACAGCTATG ACATCTACAT CCCTGTACAT
    ATCCAGAGCC AAATCACACC CCACGCCATC GTCTTTCTCC CCAACACTGA CGGCATGGAG
    ATGCTGCTTT GCTATGAAGA TGAGGGTGTC TACGTCAACA CTTACGGGCG GATCATCAAG
    GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTCG CCTACATCTG CTCCAACCAG
    ATAATGGGCT GGGGTGAGAA GGCCATAGAG ATCCGCTCTG TGGAGACAGG CCACCTAGAT
    GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAATGATAAG
    GTGTTTTTTG CCTCAGTCCG CTCTGGAGGA AGCAGCCAAG TTTACTTTAT GACTCTGAAC
    CGTAACTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246783.1
    CDS225..4268
    Translation

    Target ORF information:

    RefSeq Version XM_006246721.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246721.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TATATTCGTC ACAGGCTAGA GGAGGAGCAG
    CGACAGCTCG AGATCCTTCA GCAACAGCTG CTCCAGGAAC AGGCCTTGCT GCTGGAATAC
    AAGCGGAAGC AGCTGGAGGA GCAGCGGCAG TCGGAGCGGC TCCAGAGACA GCTGCAGCAG
    GAGCATGCCT ACCTCAAGTC CCTGCAGCAG CAGCAGCAAC AGCAGCAGCA GCTCCAGAAA
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGCAGAT CCTACCTGGA
    GACAGGAAAC CCCTGTATCA TTATGGTCGG GGCATTAATC CTGCTGACAA GCCAGCATGG
    GCCCGAGAGG TGGAAGAGAG AGCTCGGATG AACAAGCAGC AGAACTCTCC CTTGGCCAAG
    ACAAAGCCAA GCAGTGCGGG GCCAGAGCCC CCCATTCCCC AGGCCTCTCC TAGCCCCCCA
    GGACCTCTTT CCCAAACTCC TCCTATGCAG AGGCCTGTGG AGCCTCAGGA AGGACCACAC
    AAGAGCCTGG TGGCACACCG GGTCCCACTG AAGCCATATG CAGCACCTGT ACCCCGATCC
    CAGTCCCTGC AGGACCAACC CACTCGAAAC TTGGCTGCCT TCCCAGCCTC CCACGACCCT
    GACCCTGCTG CTGTCCCCAC ACCCACTGCC ACACCCAGTG CCCGAGGAGC TGTCATCCGC
    CAGAATTCAG ACCCCACCTC TGAAGGGCCT GGGCCTAGCC CTAACCCTCC ATCCTGGGTC
    CGGCCTGATA ATGAAGCTCC ACCCAAGGTT CCTCAGAGGA CCTCGTCTAT TGCCACTGCC
    CTTAACACCA GTGGGGCTGG AGGGTCCCGG CCAGCTCAGG CTGTCCGTGC CAGTAACCCT
    GACCTCAGGA GGAGTGACCC TGGCTGGGAG CGTTCAGACA GTGTCCTCCC TGCCTCCCAC
    GGCCACCTCC CTCAGGCCGG CTCCTTGGAG CGGAACCGAA ACCGTGTGGG AGCCTCCACA
    AAACTGGACA GCTCCCCAGT GCTCTCCCCT GGGAACAAGG CCAAGCCTGA AGACCACCGC
    TCAAGGCCAG GCCGGCCCGC AAGCTATAAG CGAGCGATTG GTGAGGATTT CGTGTTGCTC
    AAAGAGCGGA CTCTGGATGA GGCCCCTAAG CCTCCCAAGA AGGCCATGGA CTATTCCTCA
    TCCAGTGAGG AGGTGGAGAG CAGTGAAGAT GAGGAGGAGG AAGGCGATGG GGAGCCATCA
    GAGGGGAGCA GAGACACTCC CGGGGGCCGC AGTGATGGTG ACACAGATAG CGTCAGCACC
    ATGGTGGTTC ATGATGTTGA AGAGGTATCC GGGACCCAGC CTTCATATGG AGGAGGCACC
    ATGGTGGTCC AGCGTACTCC TGAAGAGGAG CGAAGCCTGC TGCTTGCTGA TAGCAATGGC
    TACACAAACC TGCCAGATGT GGTCCAGCCC AGCCACTCAC CTACTGAGAA CAGCCAAGGT
    CAAAGCCCTC CAACAAAGGA TGGAGGCGGT GATTACCAGT CTCGTGGGCT GGTAAAGGCC
    CCAGGAAAGA GTTCATTCAC CATGTTTGTG GATCTAGGGA TCTACCAGCC TGGAGGCAGT
    GGGGATACCA TCCCCATCAC AGCCCTAGTG GGCGGAGAGG GTGGTCGCCT TGATCAACTG
    CAGTTCGATG TGAGGAAGGG CTCTGTGGTC AACGTCAATC CCACCAACAC TCGAGCTCAT
    AGTGAAACTC CCGAGATTCG CAAGTACAAG AAGCGATTCA ATTCCGAGAT CCTCTGTGCA
    GCCCTCTGGG GGGTCAACCT CCTAGTGGGC ACAGAGAATG GGCTGATGTT GCTGGACCGA
    AGTGGGCAGG GCAAAGTGTA TGGACTTATT GGGCGACGAC GCTTCCAGCA AATGGATGTT
    CTGGAAGGGC TCAACCTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTATAT
    TACCTGTCCT GGCTTCGGAA TAAGATTCTA CACAATGACC CAGAGGTGGA AAAGAAGCAG
    GGGTGGACCA CCGTGGGGGA CATGGAGGGC TGCGGCCACT ATCGTGTTGT GAAATATGAA
    CGAATTAAGT TCCTGGTCAT TGCTCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCT
    AAACCCTACC ACAAATTCAT GGCCTTCAAG TCCTTTGCTG ACCTCCCTCA CCGCCCTCTG
    CTGGTGGACC TGACAGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGTGCT
    GGCTTCCATG CTGTGGATGT TGACTCTGGG AACAGCTATG ACATCTACAT CCCTGTACAT
    ATCCAGAGCC AAATCACACC CCACGCCATC GTCTTTCTCC CCAACACTGA CGGCATGGAG
    ATGCTGCTTT GCTATGAAGA TGAGGGTGTC TACGTCAACA CTTACGGGCG GATCATCAAG
    GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTCG CCTACATCTG CTCCAACCAG
    ATAATGGGCT GGGGTGAGAA GGCCATAGAG ATCCGCTCTG TGGAGACAGG CCACCTAGAT
    GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAATGATAAG
    GTGTTTTTTG CCTCAGTCCG CTCTGGAGGA AGCAGCCAAG TTTACTTTAT GACTCTGAAC
    CGTAACTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33328
    Clone ID Related Accession (Same CDS sequence) XM_006246722.3
    Accession Version XM_006246722.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4044bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAGATTT CGTGTTGCTC
    AAAGAGCGGA CTCTGGATGA GGCCCCTAAG CCTCCCAAGA AGGCCATGGA CTATTCCTCA
    TCCAGTGAGG AGGTGGAGAG CAGTGAAGAT GAGGAGGAGG AAGGCGATGG GGAGCCATCA
    GAGGGGAGCA GAGACACTCC CGGGGGCCGC AGTGATGGTG ACACAGATAG CGTCAGCACC
    ATGGTGGTTC ATGATGTTGA AGAGGTATCC GGGACCCAGC CTTCATATGG AGGAGGCACC
    ATGGTGGTCC AGCGTACTCC TGAAGAGGAG CGAAGCCTGC TGCTTGCTGA TAGCAATGGC
    TACACAAACC TGCCAGATGT GGTCCAGCCC AGCCACTCAC CTACTGAGAA CAGCCAAGGT
    CAAAGCCCTC CAACAAAGGA TGGAGGCGGT GATTACCAGT CTCGTGGGCT GGTAAAGGCC
    CCAGGAAAGA GTTCATTCAC CATGTTTGTG GATCTAGGGA TCTACCAGCC TGGAGGCAGT
    GGGGATACCA TCCCCATCAC AGCCCTAGTG GGCGGAGAGG GTGGTCGCCT TGATCAACTG
    CAGTTCGATG TGAGGAAGGG CTCTGTGGTC AACGTCAATC CCACCAACAC TCGAGCTCAT
    AGTGAAACTC CCGAGATTCG CAAGTACAAG AAGCGATTCA ATTCCGAGAT CCTCTGTGCA
    GCCCTCTGGG GGGTCAACCT CCTAGTGGGC ACAGAGAATG GGCTGATGTT GCTGGACCGA
    AGTGGGCAGG GCAAAGTGTA TGGACTTATT GGGCGACGAC GCTTCCAGCA AATGGATGTT
    CTGGAAGGGC TCAACCTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTATAT
    TACCTGTCCT GGCTTCGGAA TAAGATTCTA CACAATGACC CAGAGGTGGA AAAGAAGCAG
    GGGTGGACCA CCGTGGGGGA CATGGAGGGC TGCGGCCACT ATCGTGTTGT GAAATATGAA
    CGAATTAAGT TCCTGGTCAT TGCTCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCT
    AAACCCTACC ACAAATTCAT GGCCTTCAAG TCCTTTGCTG ACCTCCCTCA CCGCCCTCTG
    CTGGTGGACC TGACAGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGTGCT
    GGCTTCCATG CTGTGGATGT TGACTCTGGG AACAGCTATG ACATCTACAT CCCTGTACAT
    ATCCAGAGCC AAATCACACC CCACGCCATC GTCTTTCTCC CCAACACTGA CGGCATGGAG
    ATGCTGCTTT GCTATGAAGA TGAGGGTGTC TACGTCAACA CTTACGGGCG GATCATCAAG
    GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTCG CCTACATCTG CTCCAACCAG
    ATAATGGGCT GGGGTGAGAA GGCCATAGAG ATCCGCTCTG TGGAGACAGG CCACCTAGAT
    GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAATGATAAG
    GTGTTTTTTG CCTCAGTCCG CTCTGGAGGA AGCAGCCAAG TTTACTTTAT GACTCTGAAC
    CGTAACTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246784.1
    CDS225..4268
    Translation

    Target ORF information:

    RefSeq Version XM_006246722.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246722.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGACCT CGCAGCAACT CCGCCTGGCA AATCTATCTG
    CAGAGGCGGG CAGAGCGAGG CACCCCCAAG CCTCCTGGGC CCCCAGCTCA GCCCCCTGGC
    CCGCCCAACA CCTCTAGTAA CCCTGACCTC AGGAGGAGTG ACCCTGGCTG GGAGCGTTCA
    GACAGTGTCC TCCCTGCCTC CCACGGCCAC CTCCCTCAGG CCGGCTCCTT GGAGCGGAAC
    CGAAACCGTG TGGGAGCCTC CACAAAACTG GACAGCTCCC CAGTGCTCTC CCCTGGGAAC
    AAGGCCAAGC CTGAAGACCA CCGCTCAAGG CCAGGCCGGC CCGCAGATTT CGTGTTGCTC
    AAAGAGCGGA CTCTGGATGA GGCCCCTAAG CCTCCCAAGA AGGCCATGGA CTATTCCTCA
    TCCAGTGAGG AGGTGGAGAG CAGTGAAGAT GAGGAGGAGG AAGGCGATGG GGAGCCATCA
    GAGGGGAGCA GAGACACTCC CGGGGGCCGC AGTGATGGTG ACACAGATAG CGTCAGCACC
    ATGGTGGTTC ATGATGTTGA AGAGGTATCC GGGACCCAGC CTTCATATGG AGGAGGCACC
    ATGGTGGTCC AGCGTACTCC TGAAGAGGAG CGAAGCCTGC TGCTTGCTGA TAGCAATGGC
    TACACAAACC TGCCAGATGT GGTCCAGCCC AGCCACTCAC CTACTGAGAA CAGCCAAGGT
    CAAAGCCCTC CAACAAAGGA TGGAGGCGGT GATTACCAGT CTCGTGGGCT GGTAAAGGCC
    CCAGGAAAGA GTTCATTCAC CATGTTTGTG GATCTAGGGA TCTACCAGCC TGGAGGCAGT
    GGGGATACCA TCCCCATCAC AGCCCTAGTG GGCGGAGAGG GTGGTCGCCT TGATCAACTG
    CAGTTCGATG TGAGGAAGGG CTCTGTGGTC AACGTCAATC CCACCAACAC TCGAGCTCAT
    AGTGAAACTC CCGAGATTCG CAAGTACAAG AAGCGATTCA ATTCCGAGAT CCTCTGTGCA
    GCCCTCTGGG GGGTCAACCT CCTAGTGGGC ACAGAGAATG GGCTGATGTT GCTGGACCGA
    AGTGGGCAGG GCAAAGTGTA TGGACTTATT GGGCGACGAC GCTTCCAGCA AATGGATGTT
    CTGGAAGGGC TCAACCTGCT CATCACCATC TCAGGGAAAA GGAACAAACT GCGGGTATAT
    TACCTGTCCT GGCTTCGGAA TAAGATTCTA CACAATGACC CAGAGGTGGA AAAGAAGCAG
    GGGTGGACCA CCGTGGGGGA CATGGAGGGC TGCGGCCACT ATCGTGTTGT GAAATATGAA
    CGAATTAAGT TCCTGGTCAT TGCTCTGAAG AACTCTGTGG AGGTGTATGC CTGGGCCCCT
    AAACCCTACC ACAAATTCAT GGCCTTCAAG TCCTTTGCTG ACCTCCCTCA CCGCCCTCTG
    CTGGTGGACC TGACAGTAGA GGAGGGCCAG CGGCTCAAGG TCATCTATGG CTCCAGTGCT
    GGCTTCCATG CTGTGGATGT TGACTCTGGG AACAGCTATG ACATCTACAT CCCTGTACAT
    ATCCAGAGCC AAATCACACC CCACGCCATC GTCTTTCTCC CCAACACTGA CGGCATGGAG
    ATGCTGCTTT GCTATGAAGA TGAGGGTGTC TACGTCAACA CTTACGGGCG GATCATCAAG
    GACGTGGTGC TGCAGTGGGG AGAGATGCCC ACCTCTGTCG CCTACATCTG CTCCAACCAG
    ATAATGGGCT GGGGTGAGAA GGCCATAGAG ATCCGCTCTG TGGAGACAGG CCACCTAGAT
    GGGGTCTTCA TGCACAAACG AGCCCAGAGG CTCAAGTTCC TGTGTGAGCG GAATGATAAG
    GTGTTTTTTG CCTCAGTCCG CTCTGGAGGA AGCAGCCAAG TTTACTTTAT GACTCTGAAC
    CGTAACTGCA TCATGAACTG GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33329
    Clone ID Related Accession (Same CDS sequence) XM_006246723.3
    Accession Version XM_006246723.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC CCTGACCTCA GGAGGAGTGA CCCTGGCTGG
    GAGCGTTCAG ACAGTGTCCT CCCTGCCTCC CACGGCCACC TCCCTCAGGC CGGCTCCTTG
    GAGCGGAACC GAAACCGTGT GGGAGCCTCC ACAAAACTGG ACAGCTCCCC AGTGCTCTCC
    CCTGGGAACA AGGCCAAGCC TGAAGACCAC CGCTCAAGGC CAGGCCGGCC CGCAAGCTAT
    AAGCGAGCGA TTGGTGAGGA TTTCGTGTTG CTCAAAGAGC GGACTCTGGA TGAGGCCCCT
    AAGCCTCCCA AGAAGGCCAT GGACTATTCC TCATCCAGTG AGGAGGTGGA GAGCAGTGAA
    GATGAGGAGG AGGAAGGCGA TGGGGAGCCA TCAGAGGGGA GCAGAGACAC TCCCGGGGGC
    CGCAGTGATG GTGACACAGA TAGCGTCAGC ACCATGGTGG TTCATGATGT TGAAGAGGTA
    TCCGGGACCC AGCCTTCATA TGGAGGAGGC ACCATGGTGG TCCAGCGTAC TCCTGAAGAG
    GAGCGAAGCC TGCTGCTTGC TGATAGCAAT GGCTACACAA ACCTGCCAGA TGTGGTCCAG
    CCCAGCCACT CACCTACTGA GAACAGCCAA GGTCAAAGCC CTCCAACAAA GGATGGAGGC
    GGTGATTACC AGTCTCGTGG GCTGGTAAAG GCCCCAGGAA AGAGTTCATT CACCATGTTT
    GTGGATCTAG GGATCTACCA GCCTGGAGGC AGTGGGGATA CCATCCCCAT CACAGCCCTA
    GTGGGCGGAG AGGGTGGTCG CCTTGATCAA CTGCAGTTCG ATGTGAGGAA GGGCTCTGTG
    GTCAACGTCA ATCCCACCAA CACTCGAGCT CATAGTGAAA CTCCCGAGAT TCGCAAGTAC
    AAGAAGCGAT TCAATTCCGA GATCCTCTGT GCAGCCCTCT GGGGGGTCAA CCTCCTAGTG
    GGCACAGAGA ATGGGCTGAT GTTGCTGGAC CGAAGTGGGC AGGGCAAAGT GTATGGACTT
    ATTGGGCGAC GACGCTTCCA GCAAATGGAT GTTCTGGAAG GGCTCAACCT GCTCATCACC
    ATCTCAGGGA AAAGGAACAA ACTGCGGGTA TATTACCTGT CCTGGCTTCG GAATAAGATT
    CTACACAATG ACCCAGAGGT GGAAAAGAAG CAGGGGTGGA CCACCGTGGG GGACATGGAG
    GGCTGCGGCC ACTATCGTGT TGTGAAATAT GAACGAATTA AGTTCCTGGT CATTGCTCTG
    AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCTAAACCCT ACCACAAATT CATGGCCTTC
    AAGTCCTTTG CTGACCTCCC TCACCGCCCT CTGCTGGTGG ACCTGACAGT AGAGGAGGGC
    CAGCGGCTCA AGGTCATCTA TGGCTCCAGT GCTGGCTTCC ATGCTGTGGA TGTTGACTCT
    GGGAACAGCT ATGACATCTA CATCCCTGTA CATATCCAGA GCCAAATCAC ACCCCACGCC
    ATCGTCTTTC TCCCCAACAC TGACGGCATG GAGATGCTGC TTTGCTATGA AGATGAGGGT
    GTCTACGTCA ACACTTACGG GCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG
    CCCACCTCTG TCGCCTACAT CTGCTCCAAC CAGATAATGG GCTGGGGTGA GAAGGCCATA
    GAGATCCGCT CTGTGGAGAC AGGCCACCTA GATGGGGTCT TCATGCACAA ACGAGCCCAG
    AGGCTCAAGT TCCTGTGTGA GCGGAATGAT AAGGTGTTTT TTGCCTCAGT CCGCTCTGGA
    GGAAGCAGCC AAGTTTACTT TATGACTCTG AACCGTAACT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246785.1
    CDS225..4181
    Translation

    Target ORF information:

    RefSeq Version XM_006246723.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246723.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC CCTGACCTCA GGAGGAGTGA CCCTGGCTGG
    GAGCGTTCAG ACAGTGTCCT CCCTGCCTCC CACGGCCACC TCCCTCAGGC CGGCTCCTTG
    GAGCGGAACC GAAACCGTGT GGGAGCCTCC ACAAAACTGG ACAGCTCCCC AGTGCTCTCC
    CCTGGGAACA AGGCCAAGCC TGAAGACCAC CGCTCAAGGC CAGGCCGGCC CGCAAGCTAT
    AAGCGAGCGA TTGGTGAGGA TTTCGTGTTG CTCAAAGAGC GGACTCTGGA TGAGGCCCCT
    AAGCCTCCCA AGAAGGCCAT GGACTATTCC TCATCCAGTG AGGAGGTGGA GAGCAGTGAA
    GATGAGGAGG AGGAAGGCGA TGGGGAGCCA TCAGAGGGGA GCAGAGACAC TCCCGGGGGC
    CGCAGTGATG GTGACACAGA TAGCGTCAGC ACCATGGTGG TTCATGATGT TGAAGAGGTA
    TCCGGGACCC AGCCTTCATA TGGAGGAGGC ACCATGGTGG TCCAGCGTAC TCCTGAAGAG
    GAGCGAAGCC TGCTGCTTGC TGATAGCAAT GGCTACACAA ACCTGCCAGA TGTGGTCCAG
    CCCAGCCACT CACCTACTGA GAACAGCCAA GGTCAAAGCC CTCCAACAAA GGATGGAGGC
    GGTGATTACC AGTCTCGTGG GCTGGTAAAG GCCCCAGGAA AGAGTTCATT CACCATGTTT
    GTGGATCTAG GGATCTACCA GCCTGGAGGC AGTGGGGATA CCATCCCCAT CACAGCCCTA
    GTGGGCGGAG AGGGTGGTCG CCTTGATCAA CTGCAGTTCG ATGTGAGGAA GGGCTCTGTG
    GTCAACGTCA ATCCCACCAA CACTCGAGCT CATAGTGAAA CTCCCGAGAT TCGCAAGTAC
    AAGAAGCGAT TCAATTCCGA GATCCTCTGT GCAGCCCTCT GGGGGGTCAA CCTCCTAGTG
    GGCACAGAGA ATGGGCTGAT GTTGCTGGAC CGAAGTGGGC AGGGCAAAGT GTATGGACTT
    ATTGGGCGAC GACGCTTCCA GCAAATGGAT GTTCTGGAAG GGCTCAACCT GCTCATCACC
    ATCTCAGGGA AAAGGAACAA ACTGCGGGTA TATTACCTGT CCTGGCTTCG GAATAAGATT
    CTACACAATG ACCCAGAGGT GGAAAAGAAG CAGGGGTGGA CCACCGTGGG GGACATGGAG
    GGCTGCGGCC ACTATCGTGT TGTGAAATAT GAACGAATTA AGTTCCTGGT CATTGCTCTG
    AAGAACTCTG TGGAGGTGTA TGCCTGGGCC CCTAAACCCT ACCACAAATT CATGGCCTTC
    AAGTCCTTTG CTGACCTCCC TCACCGCCCT CTGCTGGTGG ACCTGACAGT AGAGGAGGGC
    CAGCGGCTCA AGGTCATCTA TGGCTCCAGT GCTGGCTTCC ATGCTGTGGA TGTTGACTCT
    GGGAACAGCT ATGACATCTA CATCCCTGTA CATATCCAGA GCCAAATCAC ACCCCACGCC
    ATCGTCTTTC TCCCCAACAC TGACGGCATG GAGATGCTGC TTTGCTATGA AGATGAGGGT
    GTCTACGTCA ACACTTACGG GCGGATCATC AAGGACGTGG TGCTGCAGTG GGGAGAGATG
    CCCACCTCTG TCGCCTACAT CTGCTCCAAC CAGATAATGG GCTGGGGTGA GAAGGCCATA
    GAGATCCGCT CTGTGGAGAC AGGCCACCTA GATGGGGTCT TCATGCACAA ACGAGCCCAG
    AGGCTCAAGT TCCTGTGTGA GCGGAATGAT AAGGTGTTTT TTGCCTCAGT CCGCTCTGGA
    GGAAGCAGCC AAGTTTACTT TATGACTCTG AACCGTAACT GCATCATGAA CTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33330
    Clone ID Related Accession (Same CDS sequence) XM_006246724.3
    Accession Version XM_006246724.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246724.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC CCTGACCTCA GGAGGAGTGA CCCTGGCTGG
    GAGCGTTCAG ACAGTGTCCT CCCTGCCTCC CACGGCCACC TCCCTCAGGC CGGCTCCTTG
    GAGCGGAACC GAAACCGTGT GGGAGCCTCC ACAAAACTGG ACAGCTCCCC AGTGCTCTCC
    CCTGGGAACA AGGCCAAGCC TGAAGACCAC CGCTCAAGGC CAGGCCGGCC CGCAGATTTC
    GTGTTGCTCA AAGAGCGGAC TCTGGATGAG GCCCCTAAGC CTCCCAAGAA GGCCATGGAC
    TATTCCTCAT CCAGTGAGGA GGTGGAGAGC AGTGAAGATG AGGAGGAGGA AGGCGATGGG
    GAGCCATCAG AGGGGAGCAG AGACACTCCC GGGGGCCGCA GTGATGGTGA CACAGATAGC
    GTCAGCACCA TGGTGGTTCA TGATGTTGAA GAGGTATCCG GGACCCAGCC TTCATATGGA
    GGAGGCACCA TGGTGGTCCA GCGTACTCCT GAAGAGGAGC GAAGCCTGCT GCTTGCTGAT
    AGCAATGGCT ACACAAACCT GCCAGATGTG GTCCAGCCCA GCCACTCACC TACTGAGAAC
    AGCCAAGGTC AAAGCCCTCC AACAAAGGAT GGAGGCGGTG ATTACCAGTC TCGTGGGCTG
    GTAAAGGCCC CAGGAAAGAG TTCATTCACC ATGTTTGTGG ATCTAGGGAT CTACCAGCCT
    GGAGGCAGTG GGGATACCAT CCCCATCACA GCCCTAGTGG GCGGAGAGGG TGGTCGCCTT
    GATCAACTGC AGTTCGATGT GAGGAAGGGC TCTGTGGTCA ACGTCAATCC CACCAACACT
    CGAGCTCATA GTGAAACTCC CGAGATTCGC AAGTACAAGA AGCGATTCAA TTCCGAGATC
    CTCTGTGCAG CCCTCTGGGG GGTCAACCTC CTAGTGGGCA CAGAGAATGG GCTGATGTTG
    CTGGACCGAA GTGGGCAGGG CAAAGTGTAT GGACTTATTG GGCGACGACG CTTCCAGCAA
    ATGGATGTTC TGGAAGGGCT CAACCTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG
    CGGGTATATT ACCTGTCCTG GCTTCGGAAT AAGATTCTAC ACAATGACCC AGAGGTGGAA
    AAGAAGCAGG GGTGGACCAC CGTGGGGGAC ATGGAGGGCT GCGGCCACTA TCGTGTTGTG
    AAATATGAAC GAATTAAGTT CCTGGTCATT GCTCTGAAGA ACTCTGTGGA GGTGTATGCC
    TGGGCCCCTA AACCCTACCA CAAATTCATG GCCTTCAAGT CCTTTGCTGA CCTCCCTCAC
    CGCCCTCTGC TGGTGGACCT GACAGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC
    TCCAGTGCTG GCTTCCATGC TGTGGATGTT GACTCTGGGA ACAGCTATGA CATCTACATC
    CCTGTACATA TCCAGAGCCA AATCACACCC CACGCCATCG TCTTTCTCCC CAACACTGAC
    GGCATGGAGA TGCTGCTTTG CTATGAAGAT GAGGGTGTCT ACGTCAACAC TTACGGGCGG
    ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTCGC CTACATCTGC
    TCCAACCAGA TAATGGGCTG GGGTGAGAAG GCCATAGAGA TCCGCTCTGT GGAGACAGGC
    CACCTAGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG
    AATGATAAGG TGTTTTTTGC CTCAGTCCGC TCTGGAGGAA GCAGCCAAGT TTACTTTATG
    ACTCTGAACC GTAACTGCAT CATGAACTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246786.1
    CDS225..4157
    Translation

    Target ORF information:

    RefSeq Version XM_006246724.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246724.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGGCGACC CAGCCCCCGC CCGAAGCTTG GACGACATCG ACCTGTCTGC CCTGAGGGAC 
    CCTGCAGGAA TCTTTGAGCT GGTGGAGGTG GTTGGCAATG GAACCTATGG ACAGGTGTAC
    AAGGGGCGGC ACGTCAAGAC TGGGCAGCTG GCTGCCATTA AGGTCATGGA TGTCACAGAG
    GATGAGGAGG AAGAAATCAA ACAGGAAATC AACATGTTAA AGAAGTACTC TCACCATCGC
    AATATTGCCA CCTACTACGG GGCCTTCATC AAGAAGAGCC CTCCTGGGAA CGATGACCAG
    CTCTGGCTGG TGATGGAGTT CTGTGGTGCT GGCTCAGTGA CCGACCTGGT AAAGAACACG
    AAAGGAAACG CGCTGAAAGA GGATTGCATT GCGTACATCT GCAGGGAGAT TCTCAGGGGT
    CTTGCCCATC TCCATGCCCA CAAGGTGATC CACCGAGATA TCAAGGGACA AAATGTGCTG
    CTGACAGAGA ATGCTGAAGT CAAGCTAGTG GACTTTGGGG TGAGTGCTCA GCTGGACCGC
    ACTGTGGGGA GGCGGAACAC TTTCATTGGG ACCCCATACT GGATGGCTCC AGAAGTCATT
    GCCTGTGACG AGAACCCCGA TGCCACCTAT GACTACAGGA GTGACATTTG GTCTCTAGGA
    ATCACAGCCA TTGAAATGGC AGAGGGAGCC CCCCCTCTGT GTGACATGCA CCCTATGCGG
    GCTCTCTTCC TCATCCCTCG GAACCCTCCC CCCAGGCTCA AGTCAAAGAA ATGGTCTAAG
    AAGTTCACTG ACTTCATTGA CACGTGTCTC ATCAAGACTT ACCTGAGCCG ACCACCAACA
    GAACAGTTAC TCAAATTCCC CTTCATCCGA GACCAGCCCA CGGAGCGGCA GGTCCGCATC
    CAGCTCAAGG ACCACATTGA CCGCTCCCGG AAGAAGCGGG GGGAGAAAGA GGAGACAGAG
    TATGAGTACA GTGGCAGTGA AGAGGAAGAC GACAGCCATG GAGAGGAAGG CGAGCCAAGT
    TCCATCATGA ATGTGCCTGG GGAGTCCACA CTCCGCCGAG AATTCCTCAG ACTCCAACAG
    GAGAATAAGA GCAACTCAGA GGCTTTAAAG CAGCAGCAGC AGCTGCAGCA GCAGCAGCAG
    CGGGACCCTG AGGCACACAT CAAACACCTG CTGCACCAGC GGCAGCGTCG CATAGAGGAG
    CAGAAGGAGG AGCGGCGACG TGTAGAGGAG CAACAGCGGC GAGAGCGAGA ACAGCGCAAG
    CTGCAGGAGA AGGAGCAGCA GCGGCGGTTG GAAGACATGC AAGCCCTTCG TCGAGAGGAA
    GAGAGGCGGC AAGCAGAGCG GGAACAGGAA TACAAGCGGA AGCAGCTGGA GGAGCAGCGG
    CAGTCGGAGC GGCTCCAGAG ACAGCTGCAG CAGGAGCATG CCTACCTCAA GTCCCTGCAG
    CAGCAGCAGC AACAGCAGCA GCAGCTCCAG AAACAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GATCCTACCT GGAGACAGGA AACCCCTGTA TCATTATGGT
    CGGGGCATTA ATCCTGCTGA CAAGCCAGCA TGGGCCCGAG AGGTGGAAGA GAGAGCTCGG
    ATGAACAAGC AGCAGAACTC TCCCTTGGCC AAGACAAAGC CAAGCAGTGC GGGGCCAGAG
    CCCCCCATTC CCCAGGCCTC TCCTAGCCCC CCAGGACCTC TTTCCCAAAC TCCTCCTATG
    CAGAGGCCTG TGGAGCCTCA GGAAGGACCA CACAAGAGCC TGGTGGCACA CCGGGTCCCA
    CTGAAGCCAT ATGCAGCACC TGTACCCCGA TCCCAGTCCC TGCAGGACCA ACCCACTCGA
    AACTTGGCTG CCTTCCCAGC CTCCCACGAC CCTGACCCTG CTGCTGTCCC CACACCCACT
    GCCACACCCA GTGCCCGAGG AGCTGTCATC CGCCAGAATT CAGACCCCAC CTCTGAAGGG
    CCTGGGCCTA GCCCTAACCC TCCATCCTGG GTCCGGCCTG ATAATGAAGC TCCACCCAAG
    GTTCCTCAGA GGACCTCGTC TATTGCCACT GCCCTTAACA CCAGTGGGGC TGGAGGGTCC
    CGGCCAGCTC AGGCTGTCCG TGCCAGTAAC CCTGACCTCA GGAGGAGTGA CCCTGGCTGG
    GAGCGTTCAG ACAGTGTCCT CCCTGCCTCC CACGGCCACC TCCCTCAGGC CGGCTCCTTG
    GAGCGGAACC GAAACCGTGT GGGAGCCTCC ACAAAACTGG ACAGCTCCCC AGTGCTCTCC
    CCTGGGAACA AGGCCAAGCC TGAAGACCAC CGCTCAAGGC CAGGCCGGCC CGCAGATTTC
    GTGTTGCTCA AAGAGCGGAC TCTGGATGAG GCCCCTAAGC CTCCCAAGAA GGCCATGGAC
    TATTCCTCAT CCAGTGAGGA GGTGGAGAGC AGTGAAGATG AGGAGGAGGA AGGCGATGGG
    GAGCCATCAG AGGGGAGCAG AGACACTCCC GGGGGCCGCA GTGATGGTGA CACAGATAGC
    GTCAGCACCA TGGTGGTTCA TGATGTTGAA GAGGTATCCG GGACCCAGCC TTCATATGGA
    GGAGGCACCA TGGTGGTCCA GCGTACTCCT GAAGAGGAGC GAAGCCTGCT GCTTGCTGAT
    AGCAATGGCT ACACAAACCT GCCAGATGTG GTCCAGCCCA GCCACTCACC TACTGAGAAC
    AGCCAAGGTC AAAGCCCTCC AACAAAGGAT GGAGGCGGTG ATTACCAGTC TCGTGGGCTG
    GTAAAGGCCC CAGGAAAGAG TTCATTCACC ATGTTTGTGG ATCTAGGGAT CTACCAGCCT
    GGAGGCAGTG GGGATACCAT CCCCATCACA GCCCTAGTGG GCGGAGAGGG TGGTCGCCTT
    GATCAACTGC AGTTCGATGT GAGGAAGGGC TCTGTGGTCA ACGTCAATCC CACCAACACT
    CGAGCTCATA GTGAAACTCC CGAGATTCGC AAGTACAAGA AGCGATTCAA TTCCGAGATC
    CTCTGTGCAG CCCTCTGGGG GGTCAACCTC CTAGTGGGCA CAGAGAATGG GCTGATGTTG
    CTGGACCGAA GTGGGCAGGG CAAAGTGTAT GGACTTATTG GGCGACGACG CTTCCAGCAA
    ATGGATGTTC TGGAAGGGCT CAACCTGCTC ATCACCATCT CAGGGAAAAG GAACAAACTG
    CGGGTATATT ACCTGTCCTG GCTTCGGAAT AAGATTCTAC ACAATGACCC AGAGGTGGAA
    AAGAAGCAGG GGTGGACCAC CGTGGGGGAC ATGGAGGGCT GCGGCCACTA TCGTGTTGTG
    AAATATGAAC GAATTAAGTT CCTGGTCATT GCTCTGAAGA ACTCTGTGGA GGTGTATGCC
    TGGGCCCCTA AACCCTACCA CAAATTCATG GCCTTCAAGT CCTTTGCTGA CCTCCCTCAC
    CGCCCTCTGC TGGTGGACCT GACAGTAGAG GAGGGCCAGC GGCTCAAGGT CATCTATGGC
    TCCAGTGCTG GCTTCCATGC TGTGGATGTT GACTCTGGGA ACAGCTATGA CATCTACATC
    CCTGTACATA TCCAGAGCCA AATCACACCC CACGCCATCG TCTTTCTCCC CAACACTGAC
    GGCATGGAGA TGCTGCTTTG CTATGAAGAT GAGGGTGTCT ACGTCAACAC TTACGGGCGG
    ATCATCAAGG ACGTGGTGCT GCAGTGGGGA GAGATGCCCA CCTCTGTCGC CTACATCTGC
    TCCAACCAGA TAATGGGCTG GGGTGAGAAG GCCATAGAGA TCCGCTCTGT GGAGACAGGC
    CACCTAGATG GGGTCTTCAT GCACAAACGA GCCCAGAGGC TCAAGTTCCT GTGTGAGCGG
    AATGATAAGG TGTTTTTTGC CTCAGTCCGC TCTGGAGGAA GCAGCCAAGT TTACTTTATG
    ACTCTGAACC GTAACTGCAT CATGAACTGG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa33331
    Clone ID Related Accession (Same CDS sequence) XM_006246725.3
    Accession Version XM_006246725.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product misshapen-like kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246725.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CAGAGGATGA GGAGGAAGAA ATCAAACAGG AAATCAACAT GTTAAAGAAG 
    TACTCTCACC ATCGCAATAT TGCCACCTAC TACGGGGCCT TCATCAAGAA GAGCCCTCCT
    GGGAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGCTC AGTGACCGAC
    CTGGTAAAGA ACACGAAAGG AAACGCGCTG AAAGAGGATT GCATTGCGTA CATCTGCAGG
    GAGATTCTCA GGGGTCTTGC CCATCTCCAT GCCCACAAGG TGATCCACCG AGATATCAAG
    GGACAAAATG TGCTGCTGAC AGAGAATGCT GAAGTCAAGC TAGTGGACTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACTGT GGGGAGGCGG AACACTTTCA TTGGGACCCC ATACTGGATG
    GCTCCAGAAG TCATTGCCTG TGACGAGAAC CCCGATGCCA CCTATGACTA CAGGAGTGAC
    ATTTGGTCTC TAGGAATCAC AGCCATTGAA ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCTA TGCGGGCTCT CTTCCTCATC CCTCGGAACC CTCCCCCCAG GCTCAAGTCA
    AAGAAATGGT CTAAGAAGTT CACTGACTTC ATTGACACGT GTCTCATCAA GACTTACCTG
    AGCCGACCAC CAACAGAACA GTTACTCAAA TTCCCCTTCA TCCGAGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT CAAGGACCAC ATTGACCGCT CCCGGAAGAA GCGGGGGGAG
    AAAGAGGAGA CAGAGTATGA GTACAGTGGC AGTGAAGAGG AAGACGACAG CCATGGAGAG
    GAAGGCGAGC CAAGTTCCAT CATGAATGTG CCTGGGGAGT CCACACTCCG CCGAGAATTC
    CTCAGACTCC AACAGGAGAA TAAGAGCAAC TCAGAGGCTT TAAAGCAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGGGA CCCTGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGTCGCATAG AGGAGCAGAA GGAGGAGCGG CGACGTGTAG AGGAGCAACA GCGGCGAGAG
    CGAGAACAGC GCAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGTTGGAAGA CATGCAAGCC
    CTTCGTCGAG AGGAAGAGAG GCGGCAAGCA GAGCGGGAAC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    TTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCGGA GCGGCTCCAG
    AGACAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA GCAACAGCAG
    CAGCAGCTCC AGAAACAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGATCCTAC CTGGAGACAG GAAACCCCTG TATCATTATG GTCGGGGCAT TAATCCTGCT
    GACAAGCCAG CATGGGCCCG AGAGGTGGAA GAGAGAGCTC GGATGAACAA GCAGCAGAAC
    TCTCCCTTGG CCAAGACAAA GCCAAGCAGT GCGGGGCCAG AGCCCCCCAT TCCCCAGGCC
    TCTCCTAGCC CCCCAGGACC TCTTTCCCAA ACTCCTCCTA TGCAGAGGCC TGTGGAGCCT
    CAGGAAGGAC CACACAAGAG CCTGGTGGCA CACCGGGTCC CACTGAAGCC ATATGCAGCA
    CCTGTACCCC GATCCCAGTC CCTGCAGGAC CAACCCACTC GAAACTTGGC TGCCTTCCCA
    GCCTCCCACG ACCCTGACCC TGCTGCTGTC CCCACACCCA CTGCCACACC CAGTGCCCGA
    GGAGCTGTCA TCCGCCAGAA TTCAGACCCC ACCTCTGAAG GGCCTGGGCC TAGCCCTAAC
    CCTCCATCCT GGGTCCGGCC TGATAATGAA GCTCCACCCA AGGTTCCTCA GAGGACCTCG
    TCTATTGCCA CTGCCCTTAA CACCAGTGGG GCTGGAGGGT CCCGGCCAGC TCAGGCTGTC
    CGTGCCAGAC CTCGCAGCAA CTCCGCCTGG CAAATCTATC TGCAGAGGCG GGCAGAGCGA
    GGCACCCCCA AGCCTCCTGG GCCCCCAGCT CAGCCCCCTG GCCCGCCCAA CACCTCTAGT
    AACCCTGACC TCAGGAGGAG TGACCCTGGC TGGGAGCGTT CAGACAGTGT CCTCCCTGCC
    TCCCACGGCC ACCTCCCTCA GGCCGGCTCC TTGGAGCGGA ACCGAAACCG TGTGGGAGCC
    TCCACAAAAC TGGACAGCTC CCCAGTGCTC TCCCCTGGGA ACAAGGCCAA GCCTGAAGAC
    CACCGCTCAA GGCCAGGCCG GCCCGCAAGC TATAAGCGAG CGATTGGTGA GGATTTCGTG
    TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAT
    TCCTCATCCA GTGAGGAGGT GGAGAGCAGT GAAGATGAGG AGGAGGAAGG CGATGGGGAG
    CCATCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGACAC AGATAGCGTC
    AGCACCATGG TGGTTCATGA TGTTGAAGAG GTATCCGGGA CCCAGCCTTC ATATGGAGGA
    GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAGCGAA GCCTGCTGCT TGCTGATAGC
    AATGGCTACA CAAACCTGCC AGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC
    CAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCGGTGATT ACCAGTCTCG TGGGCTGGTA
    AAGGCCCCAG GAAAGAGTTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA
    GGCAGTGGGG ATACCATCCC CATCACAGCC CTAGTGGGCG GAGAGGGTGG TCGCCTTGAT
    CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACTCGA
    GCTCATAGTG AAACTCCCGA GATTCGCAAG TACAAGAAGC GATTCAATTC CGAGATCCTC
    TGTGCAGCCC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG
    GACCGAAGTG GGCAGGGCAA AGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG
    GATGTTCTGG AAGGGCTCAA CCTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG
    GTATATTACC TGTCCTGGCT TCGGAATAAG ATTCTACACA ATGACCCAGA GGTGGAAAAG
    AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTATCG TGTTGTGAAA
    TATGAACGAA TTAAGTTCCT GGTCATTGCT CTGAAGAACT CTGTGGAGGT GTATGCCTGG
    GCCCCTAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC
    CCTCTGCTGG TGGACCTGAC AGTAGAGGAG GGCCAGCGGC TCAAGGTCAT CTATGGCTCC
    AGTGCTGGCT TCCATGCTGT GGATGTTGAC TCTGGGAACA GCTATGACAT CTACATCCCT
    GTACATATCC AGAGCCAAAT CACACCCCAC GCCATCGTCT TTCTCCCCAA CACTGACGGC
    ATGGAGATGC TGCTTTGCTA TGAAGATGAG GGTGTCTACG TCAACACTTA CGGGCGGATC
    ATCAAGGACG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTCGCCTA CATCTGCTCC
    AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC
    CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGGAAT
    GATAAGGTGT TTTTTGCCTC AGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT
    CTGAACCGTA ACTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006246787.1
    CDS118..4107
    Translation

    Target ORF information:

    RefSeq Version XM_006246725.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus misshapen-like kinase 1 (Mink1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006246725.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTCA CAGAGGATGA GGAGGAAGAA ATCAAACAGG AAATCAACAT GTTAAAGAAG 
    TACTCTCACC ATCGCAATAT TGCCACCTAC TACGGGGCCT TCATCAAGAA GAGCCCTCCT
    GGGAACGATG ACCAGCTCTG GCTGGTGATG GAGTTCTGTG GTGCTGGCTC AGTGACCGAC
    CTGGTAAAGA ACACGAAAGG AAACGCGCTG AAAGAGGATT GCATTGCGTA CATCTGCAGG
    GAGATTCTCA GGGGTCTTGC CCATCTCCAT GCCCACAAGG TGATCCACCG AGATATCAAG
    GGACAAAATG TGCTGCTGAC AGAGAATGCT GAAGTCAAGC TAGTGGACTT TGGGGTGAGT
    GCTCAGCTGG ACCGCACTGT GGGGAGGCGG AACACTTTCA TTGGGACCCC ATACTGGATG
    GCTCCAGAAG TCATTGCCTG TGACGAGAAC CCCGATGCCA CCTATGACTA CAGGAGTGAC
    ATTTGGTCTC TAGGAATCAC AGCCATTGAA ATGGCAGAGG GAGCCCCCCC TCTGTGTGAC
    ATGCACCCTA TGCGGGCTCT CTTCCTCATC CCTCGGAACC CTCCCCCCAG GCTCAAGTCA
    AAGAAATGGT CTAAGAAGTT CACTGACTTC ATTGACACGT GTCTCATCAA GACTTACCTG
    AGCCGACCAC CAACAGAACA GTTACTCAAA TTCCCCTTCA TCCGAGACCA GCCCACGGAG
    CGGCAGGTCC GCATCCAGCT CAAGGACCAC ATTGACCGCT CCCGGAAGAA GCGGGGGGAG
    AAAGAGGAGA CAGAGTATGA GTACAGTGGC AGTGAAGAGG AAGACGACAG CCATGGAGAG
    GAAGGCGAGC CAAGTTCCAT CATGAATGTG CCTGGGGAGT CCACACTCCG CCGAGAATTC
    CTCAGACTCC AACAGGAGAA TAAGAGCAAC TCAGAGGCTT TAAAGCAGCA GCAGCAGCTG
    CAGCAGCAGC AGCAGCGGGA CCCTGAGGCA CACATCAAAC ACCTGCTGCA CCAGCGGCAG
    CGTCGCATAG AGGAGCAGAA GGAGGAGCGG CGACGTGTAG AGGAGCAACA GCGGCGAGAG
    CGAGAACAGC GCAAGCTGCA GGAGAAGGAG CAGCAGCGGC GGTTGGAAGA CATGCAAGCC
    CTTCGTCGAG AGGAAGAGAG GCGGCAAGCA GAGCGGGAAC AGGAATATAT TCGTCACAGG
    CTAGAGGAGG AGCAGCGACA GCTCGAGATC CTTCAGCAAC AGCTGCTCCA GGAACAGGCC
    TTGCTGCTGG AATACAAGCG GAAGCAGCTG GAGGAGCAGC GGCAGTCGGA GCGGCTCCAG
    AGACAGCTGC AGCAGGAGCA TGCCTACCTC AAGTCCCTGC AGCAGCAGCA GCAACAGCAG
    CAGCAGCTCC AGAAACAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGATCCTAC CTGGAGACAG GAAACCCCTG TATCATTATG GTCGGGGCAT TAATCCTGCT
    GACAAGCCAG CATGGGCCCG AGAGGTGGAA GAGAGAGCTC GGATGAACAA GCAGCAGAAC
    TCTCCCTTGG CCAAGACAAA GCCAAGCAGT GCGGGGCCAG AGCCCCCCAT TCCCCAGGCC
    TCTCCTAGCC CCCCAGGACC TCTTTCCCAA ACTCCTCCTA TGCAGAGGCC TGTGGAGCCT
    CAGGAAGGAC CACACAAGAG CCTGGTGGCA CACCGGGTCC CACTGAAGCC ATATGCAGCA
    CCTGTACCCC GATCCCAGTC CCTGCAGGAC CAACCCACTC GAAACTTGGC TGCCTTCCCA
    GCCTCCCACG ACCCTGACCC TGCTGCTGTC CCCACACCCA CTGCCACACC CAGTGCCCGA
    GGAGCTGTCA TCCGCCAGAA TTCAGACCCC ACCTCTGAAG GGCCTGGGCC TAGCCCTAAC
    CCTCCATCCT GGGTCCGGCC TGATAATGAA GCTCCACCCA AGGTTCCTCA GAGGACCTCG
    TCTATTGCCA CTGCCCTTAA CACCAGTGGG GCTGGAGGGT CCCGGCCAGC TCAGGCTGTC
    CGTGCCAGAC CTCGCAGCAA CTCCGCCTGG CAAATCTATC TGCAGAGGCG GGCAGAGCGA
    GGCACCCCCA AGCCTCCTGG GCCCCCAGCT CAGCCCCCTG GCCCGCCCAA CACCTCTAGT
    AACCCTGACC TCAGGAGGAG TGACCCTGGC TGGGAGCGTT CAGACAGTGT CCTCCCTGCC
    TCCCACGGCC ACCTCCCTCA GGCCGGCTCC TTGGAGCGGA ACCGAAACCG TGTGGGAGCC
    TCCACAAAAC TGGACAGCTC CCCAGTGCTC TCCCCTGGGA ACAAGGCCAA GCCTGAAGAC
    CACCGCTCAA GGCCAGGCCG GCCCGCAAGC TATAAGCGAG CGATTGGTGA GGATTTCGTG
    TTGCTCAAAG AGCGGACTCT GGATGAGGCC CCTAAGCCTC CCAAGAAGGC CATGGACTAT
    TCCTCATCCA GTGAGGAGGT GGAGAGCAGT GAAGATGAGG AGGAGGAAGG CGATGGGGAG
    CCATCAGAGG GGAGCAGAGA CACTCCCGGG GGCCGCAGTG ATGGTGACAC AGATAGCGTC
    AGCACCATGG TGGTTCATGA TGTTGAAGAG GTATCCGGGA CCCAGCCTTC ATATGGAGGA
    GGCACCATGG TGGTCCAGCG TACTCCTGAA GAGGAGCGAA GCCTGCTGCT TGCTGATAGC
    AATGGCTACA CAAACCTGCC AGATGTGGTC CAGCCCAGCC ACTCACCTAC TGAGAACAGC
    CAAGGTCAAA GCCCTCCAAC AAAGGATGGA GGCGGTGATT ACCAGTCTCG TGGGCTGGTA
    AAGGCCCCAG GAAAGAGTTC ATTCACCATG TTTGTGGATC TAGGGATCTA CCAGCCTGGA
    GGCAGTGGGG ATACCATCCC CATCACAGCC CTAGTGGGCG GAGAGGGTGG TCGCCTTGAT
    CAACTGCAGT TCGATGTGAG GAAGGGCTCT GTGGTCAACG TCAATCCCAC CAACACTCGA
    GCTCATAGTG AAACTCCCGA GATTCGCAAG TACAAGAAGC GATTCAATTC CGAGATCCTC
    TGTGCAGCCC TCTGGGGGGT CAACCTCCTA GTGGGCACAG AGAATGGGCT GATGTTGCTG
    GACCGAAGTG GGCAGGGCAA AGTGTATGGA CTTATTGGGC GACGACGCTT CCAGCAAATG
    GATGTTCTGG AAGGGCTCAA CCTGCTCATC ACCATCTCAG GGAAAAGGAA CAAACTGCGG
    GTATATTACC TGTCCTGGCT TCGGAATAAG ATTCTACACA ATGACCCAGA GGTGGAAAAG
    AAGCAGGGGT GGACCACCGT GGGGGACATG GAGGGCTGCG GCCACTATCG TGTTGTGAAA
    TATGAACGAA TTAAGTTCCT GGTCATTGCT CTGAAGAACT CTGTGGAGGT GTATGCCTGG
    GCCCCTAAAC CCTACCACAA ATTCATGGCC TTCAAGTCCT TTGCTGACCT CCCTCACCGC
    CCTCTGCTGG TGGACCTGAC AGTAGAGGAG GGCCAGCGGC TCAAGGTCAT CTATGGCTCC
    AGTGCTGGCT TCCATGCTGT GGATGTTGAC TCTGGGAACA GCTATGACAT CTACATCCCT
    GTACATATCC AGAGCCAAAT CACACCCCAC GCCATCGTCT TTCTCCCCAA CACTGACGGC
    ATGGAGATGC TGCTTTGCTA TGAAGATGAG GGTGTCTACG TCAACACTTA CGGGCGGATC
    ATCAAGGACG TGGTGCTGCA GTGGGGAGAG ATGCCCACCT CTGTCGCCTA CATCTGCTCC
    AACCAGATAA TGGGCTGGGG TGAGAAGGCC ATAGAGATCC GCTCTGTGGA GACAGGCCAC
    CTAGATGGGG TCTTCATGCA CAAACGAGCC CAGAGGCTCA AGTTCCTGTG TGAGCGGAAT
    GATAAGGTGT TTTTTGCCTC AGTCCGCTCT GGAGGAAGCA GCCAAGTTTA CTTTATGACT
    CTGAACCGTA ACTGCATCAT GAACTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.