ambra1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ambra1
Entrez Gene ID	100497158
Full Name	autophagy/beclin-1 regulator 1
General protein information	Preferred Names autophagy/beclin-1 regulator 1 Names activating molecule in BECN1-regulated autophagy protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093274.1	XP_017948763.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_004913386.3	XP_004913443.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_004913386.4	XP_004913443.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_004913387.3	XP_004913444.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_004913387.4	XP_004913444.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_018093275.1	XP_017948764.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_002934098.4	XP_002934144.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
XM_002934098.5	XP_002934144.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Gene Ontology

The following ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43965	XM_018093274.1 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa43966	XM_018093275.1 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa56464	XM_004913386.4 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56465	XM_004913387.4 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa56466	XM_002934098.5 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa08550	XM_004913386.3 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08551	XM_004913387.3 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa08552	XM_002934098.4 Latest version!	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ambra1 ( XM_018093274.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948763.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43965
Clone ID Related Accession (Same CDS sequence)	XM_018093274.1
Accession Version	XM_018093274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC 
GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG 
GAAGGAATCC CCTCATCATC GGAGGTACCT GGCTCAGGAG AGGGCCAGGA GGATGCTCTG 
AGCAGGATCC AGAGGCTTAT GGCTGAAGGA GGGATGACGG CCGTGGTGCA GAGAGAGCAG 
AGCACAACTA TGGCATCCAT GGGAGGCTTT GGCAACAACA TCATAGTCAG CCACAGAATC 
CACCGCGGCT CCCAGACTGC GTCTGACCCG GCCTCCCGCG CTTCTGCCCC ACGCTCCCCA 
CAGCCCTCCA CCTCCAGAGA GACGGTGGCA GAGCTAGAAC GCCTTCTTGC CCCGCCCCAA 
ACGAGTCAAT TACTCCTCCC CGAGACGCAG TTGCTGACCA ATAACAATAA TGATGGTGAA 
GTCAATGTGC AGCCTCTTAG TAGTGGCACC TTTCCTGGGG TGGAGAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948763.1
CDS	325..4275
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEDNSQQPPFLLTPAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEVPGSGEGQEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQTASDPASRASAPRSPQPSTSRETVAELERLLAPPQTSQLLLPETQLLTNNNNDGEVNVQPLSSGTFPGVER

Target ORF information:

RefSeq Version	XM_018093274.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093274.1

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC 
GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG 
GAAGGAATCC CCTCATCATC GGAGGTACCT GGCTCAGGAG AGGGCCAGGA GGATGCTCTG 
AGCAGGATCC AGAGGCTTAT GGCTGAAGGA GGGATGACGG CCGTGGTGCA GAGAGAGCAG 
AGCACAACTA TGGCATCCAT GGGAGGCTTT GGCAACAACA TCATAGTCAG CCACAGAATC 
CACCGCGGCT CCCAGACTGC GTCTGACCCG GCCTCCCGCG CTTCTGCCCC ACGCTCCCCA 
CAGCCCTCCA CCTCCAGAGA GACGGTGGCA GAGCTAGAAC GCCTTCTTGC CCCGCCCCAA 
ACGAGTCAAT TACTCCTCCC CGAGACGCAG TTGCTGACCA ATAACAATAA TGATGGTGAA 
GTCAATGTGC AGCCTCTTAG TAGTGGCACC TTTCCTGGGG TGGAGAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_018093275.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948764.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa43966
Clone ID Related Accession (Same CDS sequence)	XM_018093275.1
Accession Version	XM_018093275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC 
GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG 
GAAGGAATCC CCTCATCATC GGAGGAGAGG GCCAGGAGGA TGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948764.1
CDS	325..3912
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEDNSQQPPFLLTPAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEERARRML

Target ORF information:

RefSeq Version	XM_018093275.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093275.1

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGACAACTC ACAGCAGCCT CCCTTCTTGT TAACCCCAGC AGGGCCCAGC 
GGCACAGCAG AGACCCCTTC CAGCAGCTCC GAGGGGGCGC CATCTTCTGG AGAGTCCCAG 
GAAGGAATCC CCTCATCATC GGAGGAGAGG GCCAGGAGGA TGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_004913386.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913443.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

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CloneID	OXa56464
Clone ID Related Accession (Same CDS sequence)	XM_004913386.4
Accession Version	XM_004913386.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913386.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA 
GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG 
ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC 
AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC 
CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA 
GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG 
ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT 
GGGGTGGAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913443.1
CDS	320..4234
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEVPGSGEGQEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQTASDPASRASAPRSPQPSTSRETVAELERLLAPPQTSQLLLPETQLLTNNNNDGEVNVQPLSSGTFPGVER

Target ORF information:

RefSeq Version	XM_004913386.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913386.4

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA 
GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG 
ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC 
AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC 
CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA 
GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG 
ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT 
GGGGTGGAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_004913387.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913444.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

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CloneID	OXa56465
Clone ID Related Accession (Same CDS sequence)	XM_004913387.4
Accession Version	XM_004913387.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913387.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG 
CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA 
GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG 
GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC 
ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC 
GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA 
CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC 
AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG 
GTGGAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913444.1
CDS	321..4232
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEGPSGTAETPSSSSEGAPSSGESQEGIPSSSEVPGSGEGQEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQTASDPASRASAPRSPQPSTSRETVAELERLLAPPQTSQLLLPETQLLTNNNNDGEVNVQPLSSGTFPGVER

Target ORF information:

RefSeq Version	XM_004913387.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913387.4

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG 
CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA 
GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG 
GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC 
ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC 
GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA 
CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC 
AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG 
GTGGAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_002934098.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934144.2 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56466
Clone ID Related Accession (Same CDS sequence)	XM_002934098.5
Accession Version	XM_002934098.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934098.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG 
AGGATGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934144.2
CDS	320..3871
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEERARRML

Target ORF information:

RefSeq Version	XM_002934098.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934098.5

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGCGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACAGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGAA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGGGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCCCCGA TGGGGCGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG 
AGGATGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_004913386.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913443.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

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CloneID	OXa08550
Clone ID Related Accession (Same CDS sequence)	XM_004913386.3
Accession Version	XM_004913386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913386.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA 
GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG 
ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC 
AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC 
CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA 
GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG 
ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT 
GGGGTGGAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913443.1
CDS	325..4239
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEVPGSGEGQEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQTASDPASRASAPRSPQPSTSRETVAELERLLAPPQTSQLLLPETQLLTNNNNDGEVNVQPLSSGTFPGVER

Target ORF information:

RefSeq Version	XM_004913386.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913386.3

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGT ACCTGGCTCA 
GGAGAGGGCC AGGAGGATGC TCTGAGCAGG ATCCAGAGGC TTATGGCTGA AGGAGGGATG 
ACGGCCGTGG TGCAGAGAGA GCAGAGCACA ACTATGGCAT CCATGGGAGG CTTTGGCAAC 
AACATCATAG TCAGCCACAG AATCCACCGC GGCTCCCAGA CTGCGTCTGA CCCGGCCTCC 
CGCGCTTCTG CCCCACGCTC CCCACAGCCC TCCACCTCCA GAGAGACGGT GGCAGAGCTA 
GAACGCCTTC TTGCCCCGCC CCAAACGAGT CAATTACTCC TCCCCGAGAC GCAGTTGCTG 
ACCAATAACA ATAATGATGG TGAAGTCAAT GTGCAGCCTC TTAGTAGTGG CACCTTTCCT 
GGGGTGGAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_004913387.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004913444.1 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

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CloneID	OXa08551
Clone ID Related Accession (Same CDS sequence)	XM_004913387.3
Accession Version	XM_004913387.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913387.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG 
CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA 
GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG 
GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC 
ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC 
GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA 
CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC 
AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG 
GTGGAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004913444.1
CDS	325..4236
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEGPSGTAETPSSSSEGAPSSGESQEGIPSSSEVPGSGEGQEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRGSQTASDPASRASAPRSPQPSTSRETVAELERLLAPPQTSQLLLPETQLLTNNNNDGEVNVQPLSSGTFPGVER

Target ORF information:

RefSeq Version	XM_004913387.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004913387.3

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGGGCCCAG CGGCACAGCA GAGACCCCTT CCAGCAGCTC CGAGGGGGCG 
CCATCTTCTG GAGAGTCCCA GGAAGGAATC CCCTCATCAT CGGAGGTACC TGGCTCAGGA 
GAGGGCCAGG AGGATGCTCT GAGCAGGATC CAGAGGCTTA TGGCTGAAGG AGGGATGACG 
GCCGTGGTGC AGAGAGAGCA GAGCACAACT ATGGCATCCA TGGGAGGCTT TGGCAACAAC 
ATCATAGTCA GCCACAGAAT CCACCGCGGC TCCCAGACTG CGTCTGACCC GGCCTCCCGC 
GCTTCTGCCC CACGCTCCCC ACAGCCCTCC ACCTCCAGAG AGACGGTGGC AGAGCTAGAA 
CGCCTTCTTG CCCCGCCCCA AACGAGTCAA TTACTCCTCC CCGAGACGCA GTTGCTGACC 
AATAACAATA ATGATGGTGA AGTCAATGTG CAGCCTCTTA GTAGTGGCAC CTTTCCTGGG 
GTGGAGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ambra1 ( XM_002934098.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934144.2 Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa08552
Clone ID Related Accession (Same CDS sequence)	XM_002934098.4
Accession Version	XM_002934098.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934098.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG 
AGGATGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934144.2
CDS	325..3876
Translation	MKVVPEKNAVRILAGRERGLQAQGAQRLLQCLVEDKTRFMKWEGKKVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGRCIHSLVGHRRTPWCVTFHPTIPGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTVQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQSDEESEIPLDSAEIPIYRQRSLLQTQPVRRTPLLHNFLHMLSSRSSGTQAGEQPPVQDSATPSPPPPPPPPLPPPSNDSNQRVLYTRIRERSPGVSTPCCQDPSMLCLCIRCSSARLSSPLFPHPEAPGAPSTSSGVTSTSFSPVQTEPSHSLERQQQQHQSSAQHDHGLLSRPSAFSSVHSSTAGNTLRNLSLGPTRRSLTGQPSRYQQPSTDLAGSEWTRTVLSMGPRPEMEPMPPPRTSASSVSILSVLRQQEGETSSSVYTSATEGRGFSSSESDSSAAPPPVHPTTTRTELQCDLRRFFLEYDRLHELEPGAPGTTQGQTHEMLNNNLEPEQPGPSHQPPQAHSGDSTSNQPRGHINRCRACHNLLTFNNDTLRWERTPPTYPETAGTSTWQPPPIPPPSSFQAGLAAGSTDHPGTPRVERVDLGSMAASSNRLENGTTQEERTVGVVYNPETGHWERVYSQPASVSRAPNVSQEALPQDLQEESTEEDSLRRRLLESSLISLSRYDATGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRTSAVSSSSEAQGAVETGDLEYEEFEDNGDRSRHRTTRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKYDLPEISNASINVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEILFTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQKPHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEAFDNVCDQFWEQMNEAILQHNTPRSSERPGTSRASWRSDRDMGLINAIGLQPRNPTTSVTSQGTQTPAPQLQNAETQTEREVPESRPAPPTAEAGPSGTAETPSSSSEGAPSSGESQEGIPSSSEERARRML

Target ORF information:

RefSeq Version	XM_002934098.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis autophagy/beclin-1 regulator 1 (ambra1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934098.4

ORF Insert Sequence:

ATGAAGGTAG TGCCTGAGAA GAATGCTGTC CGGATCCTGG CTGGACGGGA GAGGGGATTG 
CAAGCTCAGG GAGCCCAACG TCTTCTCCAA TGCCTGGTGG AGGATAAGAC TCGGTTTATG 
AAATGGGAGG GCAAGAAGGT GGAGCTTCCC GACAGCCCGC GTTCTACATT CCTACTGGCT 
TTCAGCCCAG ACAGGACTCT GCTAGCTTCC ACCCATGTAA ATCACAACAT TTACATCACA 
GAGGTTAAGA CTGGGCGCTG CATCCATTCT CTCGTGGGGC ATCGGCGCAC TCCATGGTGT 
GTGACTTTCC ATCCCACCAT CCCAGGACTC ATTGCATCAG GGTGCCTTGA TGGCGAGGTT 
CGAATTTGGG ATTTACATGG CGGGAGTGAA AGCTGGTTCA CGGACAGTAA CAATGCAATC 
GCCTCCCTGG CTTTCCACCC CACAGTACAG CTGCTGCTTA TCGCCACCGC CAGCGAGATT 
CACTTCTGGG ACTGGAGCCG GCGAGAACCT TTTGCTGTGG TAAAAACGGC CAGTGAAATG 
GAGCGTGTAC GATTGGTACG ATTTGACCCC CTTGGGCATT ATCTTCTTAC TGCTATTGTA 
AACCCCTCTA ACCAGCAGAG TGATGAAGAG TCGGAGATCC CCCTGGACAG TGCAGAGATT 
CCCATATACC GGCAGCGTTC CCTGCTGCAG ACTCAGCCAG TGCGCAGGAC TCCTCTGCTA 
CACAACTTTC TGCACATGCT CTCCTCCCGA TCGTCAGGTA CCCAGGCAGG AGAGCAGCCA 
CCTGTGCAGG ATTCTGCCAC GCCGTCTCCT CCACCCCCTC CACCTCCACC ACTGCCTCCC 
CCATCAAATG ACAGCAACCA GCGGGTTTTG TATACCCGCA TCCGAGAGCG ATCCCCAGGA 
GTCAGTACTC CCTGCTGCCA AGACCCCAGT ATGTTGTGCC TCTGTATCCG CTGCTCCAGT 
GCACGGCTGT CTTCTCCTCT CTTCCCACAC CCAGAAGCAC CAGGGGCTCC TTCTACCTCT 
TCAGGGGTTA CTTCCACTTC TTTCTCCCCA GTGCAGACAG AACCCTCGCA CTCCTTGGAA 
CGGCAGCAGC AGCAGCACCA GTCCTCCGCT CAACACGATC ATGGTCTTCT GAGCCGCCCT 
TCTGCCTTCA GCAGTGTGCA CAGCAGCACA GCCGGCAACA CACTGCGTAA CCTGAGCCTG 
GGGCCTACCC GCCGGTCACT AACAGGGCAG CCTTCCCGCT ATCAGCAACC ATCCACTGAT 
TTGGCTGGTT CTGAATGGAC TCGCACTGTG CTGAGTATGG GACCTCGACC GGAGATGGAA 
CCCATGCCGC CACCCAGGAC GAGTGCCTCT TCTGTCAGCA TCTTGTCTGT TCTGCGACAG 
CAGGAGGGAG AAACGTCCTC TTCTGTATAC ACCTCAGCAA CAGAGGGGAG AGGATTCTCT 
AGCTCTGAGT CTGACAGCTC TGCCGCTCCA CCACCTGTTC ACCCCACAAC CACTCGAACT 
GAACTTCAGT GTGACCTGAG ACGCTTCTTC CTCGAGTATG ACAGGCTTCA TGAACTGGAG 
CCAGGAGCAC CGGGAACAAC ACAGGGACAG ACTCACGAGA TGCTTAACAA CAACTTAGAA 
CCTGAGCAGC CTGGGCCATC TCATCAACCC CCACAAGCTC ATTCAGGCGA CAGCACCTCC 
AACCAGCCCC GTGGACACAT TAATCGCTGC CGTGCGTGCC ACAACCTGCT GACCTTCAAC 
AACGACACAC TGCGCTGGGA AAGGACACCC CCAACTTATC CGGAGACGGC AGGAACCTCT 
ACTTGGCAGC CTCCACCCAT TCCTCCCCCA AGCTCTTTCC AGGCCGGTCT CGCCGCTGGC 
AGCACAGATC ATCCTGGAAC GCCCAGGGTG GAGAGAGTGG ACCTAGGAAG CATGGCAGCA 
AGCAGTAACC GTCTGGAAAA TGGGACTACA CAGGAGGAGA GAACAGTAGG CGTGGTGTAT 
AACCCAGAGA CTGGACACTG GGAACGTGTC TACAGCCAGC CAGCATCTGT TAGCAGAGCT 
CCGAATGTAT CCCAAGAGGC CTTACCACAA GATCTGCAGG AGGAGAGCAC AGAGGAGGAT 
TCTCTCAGAA GGAGGCTTTT GGAGTCTTCG CTGATCTCTC TATCCCGATA CGATGCCACC 
GGCTCTCGAG AGCATCCAAT CTACCCTGAC CCAGCCAGGT TGTCTCCAGC TGCCTACTAC 
GCACAAAGGA TGATCCAGTA CCTGTCACGG AGAGACAGCA TAAGGCAACG CTCTATGAGG 
TACCAGCAAA ACCGCCTGCG CACCTCTGCC GTCTCATCCT CCAGCGAGGC TCAGGGAGCA 
GTAGAGACTG GAGATTTAGA GTACGAGGAG TTTGAGGACA ATGGGGACCG CTCCCGGCAC 
AGGACTACAA GGAATGCTCG CATGTCGGCA CCATCCCTGG GGCGCTTTGT ACCTAGGAGG 
TTCCTGCTTC CGGAATACCT GCCTTACGCC GGCATTTTCC ACGAGCGGGG TCAGCCAGGA 
CTGGCCACAC ACTCCTCTGT CAACAGAGTT CTGGCAGGTG CTGTGATAGG AGACGGCCAG 
TCTGCGGTGG CCAGTAACAT TGCCAATACG ACGTACAGAC TGCAGTGGTG GGACTTCACC 
AAGTACGACC TGCCGGAAAT CAGCAACGCC TCCATCAATG TGTTGGTTCA GAACTGCAAG 
ATATACAATG ATGCCAGCTG TGACATCTCC GCGGATGGGC AACTCCTCGC AGCCTTTATC 
CCAAGCAGCC AGCGAGGCTT CCCTGATGAA GGCATCCTGG CCGTATATTC CCTGGCACCA 
CACAACTTGG GCGAGATCCT GTTCACCAAG CGTTTCGGCC CGAATGCTAT CTCAGTAAGT 
CTGTCTCCGA TGGGACGATA CGTCATGGTT GGTTTGGCCT CCCGTAGGAT TTTGCTTCAT 
CCCTCCACGG AGCATATGGT GGCTCAGGTC TTCCGCCTTC AGAAGCCTCA TGGAGGAGAA 
ACATCTATGA GGCGTGTGTT CAATGTGCTG TACCCGATGC CCGCTGACCA GAGGCGACAT 
GTCAGTATCA ACTCTGCCCG CTGGCTCCCT GAGCCAGGCC TTGGATTGGC ATACGGTACA 
AACAAGGGAG ACCTGGTGAT TTGTCGGCCA GAGGCATTTG ACAATGTGTG CGATCAGTTC 
TGGGAGCAAA TGAATGAGGC CATCCTGCAG CATAACACTC CCCGGAGCAG CGAGAGGCCT 
GGAACCAGTC GTGCTTCTTG GAGATCAGAC AGAGACATGG GTCTGATCAA TGCCATTGGC 
CTTCAGCCTC GTAATCCTAC CACCTCGGTG ACCTCGCAAG GAACCCAGAC CCCTGCTCCC 
CAGCTGCAGA ATGCAGAGAC GCAGACCGAA AGGGAGGTGC CTGAGAGCCG CCCTGCACCC 
CCAACTGCTG AGGCAGGGCC CAGCGGCACA GCAGAGACCC CTTCCAGCAG CTCCGAGGGG 
GCGCCATCTT CTGGAGAGTC CCAGGAAGGA ATCCCCTCAT CATCGGAGGA GAGGGCCAGG 
AGGATGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ambra1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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