AMBRA1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010812831.2	XP_010811133.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_005216457.3	XP_005216514.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_010812832.3	XP_010811134.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_005216457.4	XP_005216514.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
XM_010812832.2	XP_010811134.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_010812833.3	XP_010811135.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
XM_010812833.2	XP_010811135.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_024975264.1	XP_024831032.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_005216458.3	XP_005216515.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
XM_024975262.1	XP_024831030.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_024975263.1	XP_024831031.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_010812831.3	XP_010811133.2	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_024975265.1	XP_024831033.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
XM_024975266.1	XP_024831034.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X8
NM_001034522.2	NP_001029694.2	activating molecule in BECN1-regulated autophagy protein 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222782	XM_024975263.1 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222782	XM_024975262.1 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222783	XM_010812831.3 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222784	XM_010812832.3 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222785	XM_024975264.1 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222786	XM_024975265.1 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222787	XM_010812833.3 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222788	XM_005216457.4 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa222789	XM_024975266.1 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa40457	NM_001034522.2 Latest version!	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26746	XM_005216457.3 Latest version!	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa26747	XM_005216458.3 Latest version!	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa49664	XM_010812831.2 Latest version!	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49665	XM_010812832.2 Latest version!	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa49666	XM_010812833.2 Latest version!	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

AMBRA1 ( XM_024975262.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831030.1 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222782
Clone ID Related Accession (Same CDS sequence)	XM_024975262.1 , XM_024975263.1
Accession Version	XM_024975262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATCTGCTT TAAAGAAGAG TCTGGCAGGG 
AGCCGGAGAC CCGCTCCTGG GACAGCCAGG AGGCTGCTGG AGTCTTCCCT CATTTCATTA 
TCCCGTTATG ATGGAGCAGG ATCCAGAGAG CACCCAATTT ACCCAGACCC AGCGAGATTA 
TCTCCTGCTG CATACTATGC CCAGAGAATG ATCCAGTACC TGTCACGGAG AGACAGTATC 
CGCCAGCGCT CCATGCGCTA CCAGCAGAAC CGCCTCCGTT CCTCCACCTC CTCCTCTTCC 
TCAGACAACC AGGGTCCAAC CGTGGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC 
AGTGGTGACA GATCCAGGCA CCGAGCTCCT CGCAATGCCC GCATGTCTGC ACCTTCGCTT 
GGACGCTTTG TCCCAAGGCG TTTCTTGCTG CCTGAATACT TGCCTTATGC TGGGATTTTT 
CATGAACGTG GACAGCCTGG CTTGGCTACT CACTCTTCTG TTAACAGGGT CCTGGCAGGG 
GCAGTGATTG GTGATGGACA GTCTGCCGTG GCCAGTAACA TTGCCAATAC TACCTACCGG 
CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC CTCCGTGAAT 
GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC 
CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT CAGAGGGGCT TTCCTGACGA AGGCATCCTG 
GCAGTGTACT CCCTGGCCCC CCATAACCTG GGCGAAATGC TCTACACCAA GCGATTTGGT 
CCCAACGCCA TCTCAGTGAG CCTGTCCCCG ATGGGCCGGT ACGTGATGGT GGGCTTGGCC 
TCTCGGAGGA TCCTGCTGCA CCCCTCCACG GAGCACATGG TGGCCCAGGT CTTCCGGCTG 
CAGCAGGCGC ACGGTGGTGA GACCTCCATG CGGAGAGTTT TCAACGTCCT TTACCCCATG 
CCTGCTGACC AGCGGAGACA TGTCAGCATC AATTCTGCCC GGTGGCTGCC CGAGCCAGGG 
CTTGGCCTGG CCTACGGCAC CAACAAAGGA GACCTGGTGA TCTGCCGACC GGAGGCCTCA 
AACTCTGGTG TTGAGTACTA CTGGGACCAG CTGAACGAGA CGGTCTTCAC TGTTCACTCC 
AGCAGCCGAA GCAGCGAGAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC 
ATGGGCCTGA TGAATGCCAT CGGCCTGCAG CCCCGGAATC CCACCACATC GGTAACGTCT 
CAGGGCACCC AAACTCTGGC CCTGCAGCTG CAAAATGCGG AGACCCAGAC GGAGCGGGAG 
ATACAGGAGC CGGGGGTGGC GGCTTCAGGT CCTGGTGAAG GGGAGGGCTC GGACTACGGG 
GCCAGCGGGG AAGACGCGCT CAGCAGGATC CAGCGGCTGA TGGCTGAAGG GGGCATGACG 
GCCGTGGTAC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGAAACAAT 
ATCATTGTCA GCCACCGCAT TCACCGCAGC TCCCAGACGG GCACGGAGCC GGGGGCCGCG 
CACGCCCCCT CGCCGCAGCC CTCCACCTCT CGGGAACTGC TCCCGGAAGC CGGGCAGCTG 
ACAGAGCGAG GCTTGAGCCC CCGGACAGCC TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG 
GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC CCTCCTCCCG CTCACCTCCC CAGCACCGAG 
GGACCAACCC CGCCGCGCTG CGACCTGACC AACAGCAACC ACCTTCCTGA CAGCGGGGGC 
AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831030.1
CDS	345..4295
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRSALKKSLAGSRRPAPGTARRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_024975262.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975262.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATCTGCTT TAAAGAAGAG TCTGGCAGGG 
AGCCGGAGAC CCGCTCCTGG GACAGCCAGG AGGCTGCTGG AGTCTTCCCT CATTTCATTA 
TCCCGTTATG ATGGAGCAGG ATCCAGAGAG CACCCAATTT ACCCAGACCC AGCGAGATTA 
TCTCCTGCTG CATACTATGC CCAGAGAATG ATCCAGTACC TGTCACGGAG AGACAGTATC 
CGCCAGCGCT CCATGCGCTA CCAGCAGAAC CGCCTCCGTT CCTCCACCTC CTCCTCTTCC 
TCAGACAACC AGGGTCCAAC CGTGGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC 
AGTGGTGACA GATCCAGGCA CCGAGCTCCT CGCAATGCCC GCATGTCTGC ACCTTCGCTT 
GGACGCTTTG TCCCAAGGCG TTTCTTGCTG CCTGAATACT TGCCTTATGC TGGGATTTTT 
CATGAACGTG GACAGCCTGG CTTGGCTACT CACTCTTCTG TTAACAGGGT CCTGGCAGGG 
GCAGTGATTG GTGATGGACA GTCTGCCGTG GCCAGTAACA TTGCCAATAC TACCTACCGG 
CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC CTCCGTGAAT 
GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC 
CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT CAGAGGGGCT TTCCTGACGA AGGCATCCTG 
GCAGTGTACT CCCTGGCCCC CCATAACCTG GGCGAAATGC TCTACACCAA GCGATTTGGT 
CCCAACGCCA TCTCAGTGAG CCTGTCCCCG ATGGGCCGGT ACGTGATGGT GGGCTTGGCC 
TCTCGGAGGA TCCTGCTGCA CCCCTCCACG GAGCACATGG TGGCCCAGGT CTTCCGGCTG 
CAGCAGGCGC ACGGTGGTGA GACCTCCATG CGGAGAGTTT TCAACGTCCT TTACCCCATG 
CCTGCTGACC AGCGGAGACA TGTCAGCATC AATTCTGCCC GGTGGCTGCC CGAGCCAGGG 
CTTGGCCTGG CCTACGGCAC CAACAAAGGA GACCTGGTGA TCTGCCGACC GGAGGCCTCA 
AACTCTGGTG TTGAGTACTA CTGGGACCAG CTGAACGAGA CGGTCTTCAC TGTTCACTCC 
AGCAGCCGAA GCAGCGAGAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC 
ATGGGCCTGA TGAATGCCAT CGGCCTGCAG CCCCGGAATC CCACCACATC GGTAACGTCT 
CAGGGCACCC AAACTCTGGC CCTGCAGCTG CAAAATGCGG AGACCCAGAC GGAGCGGGAG 
ATACAGGAGC CGGGGGTGGC GGCTTCAGGT CCTGGTGAAG GGGAGGGCTC GGACTACGGG 
GCCAGCGGGG AAGACGCGCT CAGCAGGATC CAGCGGCTGA TGGCTGAAGG GGGCATGACG 
GCCGTGGTAC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGAAACAAT 
ATCATTGTCA GCCACCGCAT TCACCGCAGC TCCCAGACGG GCACGGAGCC GGGGGCCGCG 
CACGCCCCCT CGCCGCAGCC CTCCACCTCT CGGGAACTGC TCCCGGAAGC CGGGCAGCTG 
ACAGAGCGAG GCTTGAGCCC CCGGACAGCC TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG 
GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC CCTCCTCCCG CTCACCTCCC CAGCACCGAG 
GGACCAACCC CGCCGCGCTG CGACCTGACC AACAGCAACC ACCTTCCTGA CAGCGGGGGC 
AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_024975263.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831031.1 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222782
Clone ID Related Accession (Same CDS sequence)	XM_024975262.1 , XM_024975263.1
Accession Version	XM_024975263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATCTGCTT TAAAGAAGAG TCTGGCAGGG 
AGCCGGAGAC CCGCTCCTGG GACAGCCAGG AGGCTGCTGG AGTCTTCCCT CATTTCATTA 
TCCCGTTATG ATGGAGCAGG ATCCAGAGAG CACCCAATTT ACCCAGACCC AGCGAGATTA 
TCTCCTGCTG CATACTATGC CCAGAGAATG ATCCAGTACC TGTCACGGAG AGACAGTATC 
CGCCAGCGCT CCATGCGCTA CCAGCAGAAC CGCCTCCGTT CCTCCACCTC CTCCTCTTCC 
TCAGACAACC AGGGTCCAAC CGTGGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC 
AGTGGTGACA GATCCAGGCA CCGAGCTCCT CGCAATGCCC GCATGTCTGC ACCTTCGCTT 
GGACGCTTTG TCCCAAGGCG TTTCTTGCTG CCTGAATACT TGCCTTATGC TGGGATTTTT 
CATGAACGTG GACAGCCTGG CTTGGCTACT CACTCTTCTG TTAACAGGGT CCTGGCAGGG 
GCAGTGATTG GTGATGGACA GTCTGCCGTG GCCAGTAACA TTGCCAATAC TACCTACCGG 
CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC CTCCGTGAAT 
GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC 
CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT CAGAGGGGCT TTCCTGACGA AGGCATCCTG 
GCAGTGTACT CCCTGGCCCC CCATAACCTG GGCGAAATGC TCTACACCAA GCGATTTGGT 
CCCAACGCCA TCTCAGTGAG CCTGTCCCCG ATGGGCCGGT ACGTGATGGT GGGCTTGGCC 
TCTCGGAGGA TCCTGCTGCA CCCCTCCACG GAGCACATGG TGGCCCAGGT CTTCCGGCTG 
CAGCAGGCGC ACGGTGGTGA GACCTCCATG CGGAGAGTTT TCAACGTCCT TTACCCCATG 
CCTGCTGACC AGCGGAGACA TGTCAGCATC AATTCTGCCC GGTGGCTGCC CGAGCCAGGG 
CTTGGCCTGG CCTACGGCAC CAACAAAGGA GACCTGGTGA TCTGCCGACC GGAGGCCTCA 
AACTCTGGTG TTGAGTACTA CTGGGACCAG CTGAACGAGA CGGTCTTCAC TGTTCACTCC 
AGCAGCCGAA GCAGCGAGAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC 
ATGGGCCTGA TGAATGCCAT CGGCCTGCAG CCCCGGAATC CCACCACATC GGTAACGTCT 
CAGGGCACCC AAACTCTGGC CCTGCAGCTG CAAAATGCGG AGACCCAGAC GGAGCGGGAG 
ATACAGGAGC CGGGGGTGGC GGCTTCAGGT CCTGGTGAAG GGGAGGGCTC GGACTACGGG 
GCCAGCGGGG AAGACGCGCT CAGCAGGATC CAGCGGCTGA TGGCTGAAGG GGGCATGACG 
GCCGTGGTAC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGAAACAAT 
ATCATTGTCA GCCACCGCAT TCACCGCAGC TCCCAGACGG GCACGGAGCC GGGGGCCGCG 
CACGCCCCCT CGCCGCAGCC CTCCACCTCT CGGGAACTGC TCCCGGAAGC CGGGCAGCTG 
ACAGAGCGAG GCTTGAGCCC CCGGACAGCC TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG 
GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC CCTCCTCCCG CTCACCTCCC CAGCACCGAG 
GGACCAACCC CGCCGCGCTG CGACCTGACC AACAGCAACC ACCTTCCTGA CAGCGGGGGC 
AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831031.1
CDS	142..4092
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRSALKKSLAGSRRPAPGTARRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_024975263.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975263.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATCTGCTT TAAAGAAGAG TCTGGCAGGG 
AGCCGGAGAC CCGCTCCTGG GACAGCCAGG AGGCTGCTGG AGTCTTCCCT CATTTCATTA 
TCCCGTTATG ATGGAGCAGG ATCCAGAGAG CACCCAATTT ACCCAGACCC AGCGAGATTA 
TCTCCTGCTG CATACTATGC CCAGAGAATG ATCCAGTACC TGTCACGGAG AGACAGTATC 
CGCCAGCGCT CCATGCGCTA CCAGCAGAAC CGCCTCCGTT CCTCCACCTC CTCCTCTTCC 
TCAGACAACC AGGGTCCAAC CGTGGAGGGA ACCGACTTGG AGTTTGAGGA CTTTGAGGAC 
AGTGGTGACA GATCCAGGCA CCGAGCTCCT CGCAATGCCC GCATGTCTGC ACCTTCGCTT 
GGACGCTTTG TCCCAAGGCG TTTCTTGCTG CCTGAATACT TGCCTTATGC TGGGATTTTT 
CATGAACGTG GACAGCCTGG CTTGGCTACT CACTCTTCTG TTAACAGGGT CCTGGCAGGG 
GCAGTGATTG GTGATGGACA GTCTGCCGTG GCCAGTAACA TTGCCAATAC TACCTACCGG 
CTCCAGTGGT GGGACTTCAC TAAGTTTGAC CTCCCGGAAA TCAGTAATGC CTCCGTGAAT 
GTGCTGGTGC AGAACTGCAA GATCTACAAT GATGCCAGCT GTGACATTTC TGCAGATGGC 
CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT CAGAGGGGCT TTCCTGACGA AGGCATCCTG 
GCAGTGTACT CCCTGGCCCC CCATAACCTG GGCGAAATGC TCTACACCAA GCGATTTGGT 
CCCAACGCCA TCTCAGTGAG CCTGTCCCCG ATGGGCCGGT ACGTGATGGT GGGCTTGGCC 
TCTCGGAGGA TCCTGCTGCA CCCCTCCACG GAGCACATGG TGGCCCAGGT CTTCCGGCTG 
CAGCAGGCGC ACGGTGGTGA GACCTCCATG CGGAGAGTTT TCAACGTCCT TTACCCCATG 
CCTGCTGACC AGCGGAGACA TGTCAGCATC AATTCTGCCC GGTGGCTGCC CGAGCCAGGG 
CTTGGCCTGG CCTACGGCAC CAACAAAGGA GACCTGGTGA TCTGCCGACC GGAGGCCTCA 
AACTCTGGTG TTGAGTACTA CTGGGACCAG CTGAACGAGA CGGTCTTCAC TGTTCACTCC 
AGCAGCCGAA GCAGCGAGAG GCCTGGAACC AGCAGAGCCA CGTGGAGGAC AGACAGAGAC 
ATGGGCCTGA TGAATGCCAT CGGCCTGCAG CCCCGGAATC CCACCACATC GGTAACGTCT 
CAGGGCACCC AAACTCTGGC CCTGCAGCTG CAAAATGCGG AGACCCAGAC GGAGCGGGAG 
ATACAGGAGC CGGGGGTGGC GGCTTCAGGT CCTGGTGAAG GGGAGGGCTC GGACTACGGG 
GCCAGCGGGG AAGACGCGCT CAGCAGGATC CAGCGGCTGA TGGCTGAAGG GGGCATGACG 
GCCGTGGTAC AGCGGGAGCA GAGCACCACC ATGGCCTCCA TGGGCGGCTT CGGAAACAAT 
ATCATTGTCA GCCACCGCAT TCACCGCAGC TCCCAGACGG GCACGGAGCC GGGGGCCGCG 
CACGCCCCCT CGCCGCAGCC CTCCACCTCT CGGGAACTGC TCCCGGAAGC CGGGCAGCTG 
ACAGAGCGAG GCTTGAGCCC CCGGACAGCC TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG 
GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC CCTCCTCCCG CTCACCTCCC CAGCACCGAG 
GGACCAACCC CGCCGCGCTG CGACCTGACC AACAGCAACC ACCTTCCTGA CAGCGGGGGC 
AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812831.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811133.2 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

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CloneID	OBa222783
Clone ID Related Accession (Same CDS sequence)	XM_010812831.3
Accession Version	XM_010812831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACAGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811133.2
CDS	345..4238
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812831.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812831.3

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACAGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812832.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811134.2 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

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CloneID	OBa222784
Clone ID Related Accession (Same CDS sequence)	XM_010812832.3
Accession Version	XM_010812832.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCAACCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACAGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811134.2
CDS	345..4151
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812832.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812832.3

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCAACCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACAGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_024975264.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831032.1 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa222785
Clone ID Related Accession (Same CDS sequence)	XM_024975264.1
Accession Version	XM_024975264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCG GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCGCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCTCC 
GGGCTGACGG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCA 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTACTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC AGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACTTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGCGCCTC CTGGCAGGTC CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA ACTCTGCCAG CCCACAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGCTCTGAGG AGGATTCTCT CAGGAGATCT 
GCTTTAAAGA AGAGTCTGGC AGGGAGCCGG AGACCCGCTC CTGGGACAGC CAGGAGGCTG 
CTGGAGTCTT CCCTCATTTC ATTATCCCGT TATGATGGAG CAGGATCCAG AGAGCACCCA 
ATTTACCCAG ACCCAGCGAG ATTATCTCCT GCTGCATACT ATGCCCAGAG AATGATCCAG 
TACCTGTCAC GGAGAGACAG TATCCGCCAG CGCTCCATGC GCTACCAGCA GAACCGCCTC 
CGTTCCTCCA CCTCCTCCTC TTCCTCAGAC AACCAGGGTC CAACCGTGGA GGGAACCGAC 
TTGGAGTTTG AGGACTTTGA GGACAGTGGT GACAGATCCA GGCACCGAGC TCCTCGCAAT 
GCCCGCATGT CTGCACCTTC GCTTGGACGC TTTGTCCCAA GGCGTTTCTT GCTGCCTGAA 
TACTTGCCTT ATGCTGGGAT TTTTCATGAA CGTGGACAGC CTGGCTTGGC TACTCACTCT 
TCTGTTAACA GGGTCCTGGC AGGGGCAGTG ATTGGTGATG GACAGTCTGC CGTGGCCAGT 
AACATTGCCA ATACTACCTA CCGGCTCCAG TGGTGGGACT TCACTAAGTT TGACCTCCCG 
GAAATCAGTA ATGCCTCCGT GAATGTGCTG GTGCAGAACT GCAAGATCTA CAATGATGCC 
AGCTGTGACA TTTCTGCAGA TGGCCAGCTC CTGGCAGCGT TCATCCCTAG CAGTCAGAGG 
GGCTTTCCTG ACGAAGGCAT CCTGGCAGTG TACTCCCTGG CCCCCCATAA CCTGGGCGAA 
ATGCTCTACA CCAAGCGATT TGGTCCCAAC GCCATCTCAG TGAGCCTGTC CCCGATGGGC 
CGGTACGTGA TGGTGGGCTT GGCCTCTCGG AGGATCCTGC TGCACCCCTC CACGGAGCAC 
ATGGTGGCCC AGGTCTTCCG GCTGCAGCAG GCGCACGGTG GTGAGACCTC CATGCGGAGA 
GTTTTCAACG TCCTTTACCC CATGCCTGCT GACCAGCGGA GACATGTCAG CATCAATTCT 
GCCCGGTGGC TGCCCGAGCC AGGGCTTGGC CTGGCCTACG GCACCAACAA AGGAGACCTG 
GTGATCTGCC GACCGGAGGC CTCAAACTCT GGTGTTGAGT ACTACTGGGA CCAGCTGAAC 
GAGACGGTCT TCACTGTTCA CTCCAGCAGC CGAAGCAGCG AGAGGCCTGG AACCAGCAGA 
GCCACGTGGA GGACAGACAG AGACATGGGC CTGATGAATG CCATCGGCCT GCAGCCCCGG 
AATCCCACCA CATCGGTAAC GTCTCAGGGC ACCCAAACTC TGGCCCTGCA GCTGCAAAAT 
GCGGAGACCC AGACGGAGCG GGAGATACAG GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT 
GAAGGGGAGG GCTCGGACTA CGGGGCCAGC GGGGAAGACG CGCTCAGCAG GATCCAGCGG 
CTGATGGCTG AAGGGGGCAT GACGGCCGTG GTACAGCGGG AGCAGAGCAC CACCATGGCC 
TCCATGGGCG GCTTCGGAAA CAATATCATT GTCAGCCACC GCATTCACCG CAGCTCCCAG 
ACGGGCACGG AGCCGGGGGC CGCGCACGCC CCCTCGCCGC AGCCCTCCAC CTCTCGGGAA 
CTGCTCCCGG AAGCCGGGCA GCTGACAGAG CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG 
GAGCAGCCCG CTACCCCTGG GCGGGAGCCC GCCCTGCCCT CCTCCTCCCC CGCCCCTCCT 
CCCGCTCACC TCCCCAGCAC CGAGGGACCA ACCCCGCCGC GCTGCGACCT GACCAACAGC 
AACCACCTTC CTGACAGCGG GGGCAGCGGC AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG 
CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831032.1
CDS	345..4139
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRSALKKSLAGSRRPAPGTARRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_024975264.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975264.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGA CCGAGCCCTT CCACCCCCCG 
GAGCAGGCCT CGTCAGCGCC GCACGACCCG GGCCTCCTGA GCCGGCCGTC TGCCTTCAGT 
ACGGTCCAGA GCAGCACCGC CGGCAACACG CTCCGCAACC TCAGCCTGGG TCCCCCGCGG 
CGCTCCCTGG GAGGCCCTCT GTCTGGCCAC CCTTCCAGGT ACCACCGGGA CATAGCCTCC 
GGGCTGACGG GATCTGAGTG GACCCGGACA GTGCTCAGTC TGAACTCTCG CTCCGAGGCA 
GAGTCCATGC CCCCGCCCAG GACCAGCGCC TCTTCGGTGA GTCTACTCTC TGTGCTGCGA 
CAGCAGGAAG GTGGCTCTCA AGCCTCTGTG TACACTTCAG CCACAGAAGG GAGGGGTTTT 
CCAGCCTCGG GGTTGGCAGC TGAGTCAGAT GGAGGGAACG GCTCCAGCCA AAACAACTCG 
GGCAGCATCC GCCACGAGCT TCAGTGTGAC CTGAGACGCT TCTTTCTGGA GTATGACCGG 
CTTCAGGAGC TGGACCAGAG CCTCAGCGGG GAGGCCCCCC AGGCCCAGCA GGCCCAGGAA 
ATGCTCAACA ATAACCTTGA ATCCGAACGG CCAGGGCCGT CCCACCAGCC CACCCCGCAC 
AGCAGCGAGA ACAACTCCAA CCTGTCCCGT GGCCACTTGA ACCGCTGCCG CGCTTGCCAC 
AATCTGCTGA CCTTCAACAA CGACACTCTG CGCTGGGAAA GAAGCACACC TAACTACTCC 
TCTGGCGAGG CCAGCGCCTC CTGGCAGGTC CCCGGCACCT TTGAGGGCAT GGCCGCGAGT 
GGCAGCCAGC TGCCCCCTCT CGAGCGGACT GAGGGCCAGA CGGCCAGCTC CAGCAGGCTG 
GAGTTGGGCA ACTCTGCCAG CCCACAGGAG GAGAGGACTG TGGGGGTGGC CTTCAACCAG 
GAGACGGGCC ACTGGGAACG CATTTACACC CAGGCCAGCC GATCTGGAAC TGTGTCACAG 
GAGGCCTTAC ATCAGGATCT GCCGGAGGAG AGCTCTGAGG AGGATTCTCT CAGGAGATCT 
GCTTTAAAGA AGAGTCTGGC AGGGAGCCGG AGACCCGCTC CTGGGACAGC CAGGAGGCTG 
CTGGAGTCTT CCCTCATTTC ATTATCCCGT TATGATGGAG CAGGATCCAG AGAGCACCCA 
ATTTACCCAG ACCCAGCGAG ATTATCTCCT GCTGCATACT ATGCCCAGAG AATGATCCAG 
TACCTGTCAC GGAGAGACAG TATCCGCCAG CGCTCCATGC GCTACCAGCA GAACCGCCTC 
CGTTCCTCCA CCTCCTCCTC TTCCTCAGAC AACCAGGGTC CAACCGTGGA GGGAACCGAC 
TTGGAGTTTG AGGACTTTGA GGACAGTGGT GACAGATCCA GGCACCGAGC TCCTCGCAAT 
GCCCGCATGT CTGCACCTTC GCTTGGACGC TTTGTCCCAA GGCGTTTCTT GCTGCCTGAA 
TACTTGCCTT ATGCTGGGAT TTTTCATGAA CGTGGACAGC CTGGCTTGGC TACTCACTCT 
TCTGTTAACA GGGTCCTGGC AGGGGCAGTG ATTGGTGATG GACAGTCTGC CGTGGCCAGT 
AACATTGCCA ATACTACCTA CCGGCTCCAG TGGTGGGACT TCACTAAGTT TGACCTCCCG 
GAAATCAGTA ATGCCTCCGT GAATGTGCTG GTGCAGAACT GCAAGATCTA CAATGATGCC 
AGCTGTGACA TTTCTGCAGA TGGCCAGCTC CTGGCAGCGT TCATCCCTAG CAGTCAGAGG 
GGCTTTCCTG ACGAAGGCAT CCTGGCAGTG TACTCCCTGG CCCCCCATAA CCTGGGCGAA 
ATGCTCTACA CCAAGCGATT TGGTCCCAAC GCCATCTCAG TGAGCCTGTC CCCGATGGGC 
CGGTACGTGA TGGTGGGCTT GGCCTCTCGG AGGATCCTGC TGCACCCCTC CACGGAGCAC 
ATGGTGGCCC AGGTCTTCCG GCTGCAGCAG GCGCACGGTG GTGAGACCTC CATGCGGAGA 
GTTTTCAACG TCCTTTACCC CATGCCTGCT GACCAGCGGA GACATGTCAG CATCAATTCT 
GCCCGGTGGC TGCCCGAGCC AGGGCTTGGC CTGGCCTACG GCACCAACAA AGGAGACCTG 
GTGATCTGCC GACCGGAGGC CTCAAACTCT GGTGTTGAGT ACTACTGGGA CCAGCTGAAC 
GAGACGGTCT TCACTGTTCA CTCCAGCAGC CGAAGCAGCG AGAGGCCTGG AACCAGCAGA 
GCCACGTGGA GGACAGACAG AGACATGGGC CTGATGAATG CCATCGGCCT GCAGCCCCGG 
AATCCCACCA CATCGGTAAC GTCTCAGGGC ACCCAAACTC TGGCCCTGCA GCTGCAAAAT 
GCGGAGACCC AGACGGAGCG GGAGATACAG GAGCCGGGGG TGGCGGCTTC AGGTCCTGGT 
GAAGGGGAGG GCTCGGACTA CGGGGCCAGC GGGGAAGACG CGCTCAGCAG GATCCAGCGG 
CTGATGGCTG AAGGGGGCAT GACGGCCGTG GTACAGCGGG AGCAGAGCAC CACCATGGCC 
TCCATGGGCG GCTTCGGAAA CAATATCATT GTCAGCCACC GCATTCACCG CAGCTCCCAG 
ACGGGCACGG AGCCGGGGGC CGCGCACGCC CCCTCGCCGC AGCCCTCCAC CTCTCGGGAA 
CTGCTCCCGG AAGCCGGGCA GCTGACAGAG CGAGGCTTGA GCCCCCGGAC AGCCTCCTGG 
GAGCAGCCCG CTACCCCTGG GCGGGAGCCC GCCCTGCCCT CCTCCTCCCC CGCCCCTCCT 
CCCGCTCACC TCCCCAGCAC CGAGGGACCA ACCCCGCCGC GCTGCGACCT GACCAACAGC 
AACCACCTTC CTGACAGCGG GGGCAGCGGC AGGGGGGAGG CGGCGGGCCC CAGCGGGGAG 
CCACGGGACA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_024975265.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831033.1 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

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CloneID	OBa222786
Clone ID Related Accession (Same CDS sequence)	XM_024975265.1
Accession Version	XM_024975265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATCTGCTT TAAAGAAGAG TCTGGCAGGG AGCCGGAGAC CCGCTCCTGG GACAGCCAGG 
AGGCTGCTGG AGTCTTCCCT CATTTCATTA TCCCGTTATG ATGGAGCAGG ATCCAGAGAG 
CACCCAATTT ACCCAGACCC AGCGAGATTA TCTCCTGCTG CATACTATGC CCAGAGAATG 
ATCCAGTACC TGTCACGGAG AGACAGTATC CGCCAGCGCT CCATGCGCTA CCAGCAGAAC 
CGCCTCCGTT CCTCCACCTC CTCCTCTTCC TCAGACAACC AGGGTCCAAC CGTGGAGGGA 
ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AGTGGTGACA GATCCAGGCA CCGAGCTCCT 
CGCAATGCCC GCATGTCTGC ACCTTCGCTT GGACGCTTTG TCCCAAGGCG TTTCTTGCTG 
CCTGAATACT TGCCTTATGC TGGGATTTTT CATGAACGTG GACAGCCTGG CTTGGCTACT 
CACTCTTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCTGCCGTG 
GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC 
CTCCCGGAAA TCAGTAATGC CTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT 
GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT 
CAGAGGGGCT TTCCTGACGA AGGCATCCTG GCAGTGTACT CCCTGGCCCC CCATAACCTG 
GGCGAAATGC TCTACACCAA GCGATTTGGT CCCAACGCCA TCTCAGTGAG CCTGTCCCCG 
ATGGGCCGGT ACGTGATGGT GGGCTTGGCC TCTCGGAGGA TCCTGCTGCA CCCCTCCACG 
GAGCACATGG TGGCCCAGGT CTTCCGGCTG CAGCAGGCGC ACGGTGGTGA GACCTCCATG 
CGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC 
AATTCTGCCC GGTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTACGGCAC CAACAAAGGA 
GACCTGGTGA TCTGCCGACC GGAGGCCTCA AACTCTGGTG TTGAGTACTA CTGGGACCAG 
CTGAACGAGA CGGTCTTCAC TGTTCACTCC AGCAGCCGAA GCAGCGAGAG GCCTGGAACC 
AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGCCTGA TGAATGCCAT CGGCCTGCAG 
CCCCGGAATC CCACCACATC GGTAACGTCT CAGGGCACCC AAACTCTGGC CCTGCAGCTG 
CAAAATGCGG AGACCCAGAC GGAGCGGGAG ATACAGGAGC CGGGGGTGGC GGCTTCAGGT 
CCTGGTGAAG GGGAGGGCTC GGACTACGGG GCCAGCGGGG AAGACGCGCT CAGCAGGATC 
CAGCGGCTGA TGGCTGAAGG GGGCATGACG GCCGTGGTAC AGCGGGAGCA GAGCACCACC 
ATGGCCTCCA TGGGCGGCTT CGGAAACAAT ATCATTGTCA GCCACCGCAT TCACCGCAGC 
TCCCAGACGG GCACGGAGCC GGGGGCCGCG CACGCCCCCT CGCCGCAGCC CTCCACCTCT 
CGGGAACTGC TCCCGGAAGC CGGGCAGCTG ACAGAGCGAG GCTTGAGCCC CCGGACAGCC 
TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC 
CCTCCTCCCG CTCACCTCCC CAGCACCGAG GGACCAACCC CGCCGCGCTG CGACCTGACC 
AACAGCAACC ACCTTCCTGA CAGCGGGGGC AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC 
GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831033.1
CDS	344..4024
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRSALKKSLAGSRRPAPGTARRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_024975265.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975265.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATCTGCTT TAAAGAAGAG TCTGGCAGGG AGCCGGAGAC CCGCTCCTGG GACAGCCAGG 
AGGCTGCTGG AGTCTTCCCT CATTTCATTA TCCCGTTATG ATGGAGCAGG ATCCAGAGAG 
CACCCAATTT ACCCAGACCC AGCGAGATTA TCTCCTGCTG CATACTATGC CCAGAGAATG 
ATCCAGTACC TGTCACGGAG AGACAGTATC CGCCAGCGCT CCATGCGCTA CCAGCAGAAC 
CGCCTCCGTT CCTCCACCTC CTCCTCTTCC TCAGACAACC AGGGTCCAAC CGTGGAGGGA 
ACCGACTTGG AGTTTGAGGA CTTTGAGGAC AGTGGTGACA GATCCAGGCA CCGAGCTCCT 
CGCAATGCCC GCATGTCTGC ACCTTCGCTT GGACGCTTTG TCCCAAGGCG TTTCTTGCTG 
CCTGAATACT TGCCTTATGC TGGGATTTTT CATGAACGTG GACAGCCTGG CTTGGCTACT 
CACTCTTCTG TTAACAGGGT CCTGGCAGGG GCAGTGATTG GTGATGGACA GTCTGCCGTG 
GCCAGTAACA TTGCCAATAC TACCTACCGG CTCCAGTGGT GGGACTTCAC TAAGTTTGAC 
CTCCCGGAAA TCAGTAATGC CTCCGTGAAT GTGCTGGTGC AGAACTGCAA GATCTACAAT 
GATGCCAGCT GTGACATTTC TGCAGATGGC CAGCTCCTGG CAGCGTTCAT CCCTAGCAGT 
CAGAGGGGCT TTCCTGACGA AGGCATCCTG GCAGTGTACT CCCTGGCCCC CCATAACCTG 
GGCGAAATGC TCTACACCAA GCGATTTGGT CCCAACGCCA TCTCAGTGAG CCTGTCCCCG 
ATGGGCCGGT ACGTGATGGT GGGCTTGGCC TCTCGGAGGA TCCTGCTGCA CCCCTCCACG 
GAGCACATGG TGGCCCAGGT CTTCCGGCTG CAGCAGGCGC ACGGTGGTGA GACCTCCATG 
CGGAGAGTTT TCAACGTCCT TTACCCCATG CCTGCTGACC AGCGGAGACA TGTCAGCATC 
AATTCTGCCC GGTGGCTGCC CGAGCCAGGG CTTGGCCTGG CCTACGGCAC CAACAAAGGA 
GACCTGGTGA TCTGCCGACC GGAGGCCTCA AACTCTGGTG TTGAGTACTA CTGGGACCAG 
CTGAACGAGA CGGTCTTCAC TGTTCACTCC AGCAGCCGAA GCAGCGAGAG GCCTGGAACC 
AGCAGAGCCA CGTGGAGGAC AGACAGAGAC ATGGGCCTGA TGAATGCCAT CGGCCTGCAG 
CCCCGGAATC CCACCACATC GGTAACGTCT CAGGGCACCC AAACTCTGGC CCTGCAGCTG 
CAAAATGCGG AGACCCAGAC GGAGCGGGAG ATACAGGAGC CGGGGGTGGC GGCTTCAGGT 
CCTGGTGAAG GGGAGGGCTC GGACTACGGG GCCAGCGGGG AAGACGCGCT CAGCAGGATC 
CAGCGGCTGA TGGCTGAAGG GGGCATGACG GCCGTGGTAC AGCGGGAGCA GAGCACCACC 
ATGGCCTCCA TGGGCGGCTT CGGAAACAAT ATCATTGTCA GCCACCGCAT TCACCGCAGC 
TCCCAGACGG GCACGGAGCC GGGGGCCGCG CACGCCCCCT CGCCGCAGCC CTCCACCTCT 
CGGGAACTGC TCCCGGAAGC CGGGCAGCTG ACAGAGCGAG GCTTGAGCCC CCGGACAGCC 
TCCTGGGAGC AGCCCGCTAC CCCTGGGCGG GAGCCCGCCC TGCCCTCCTC CTCCCCCGCC 
CCTCCTCCCG CTCACCTCCC CAGCACCGAG GGACCAACCC CGCCGCGCTG CGACCTGACC 
AACAGCAACC ACCTTCCTGA CAGCGGGGGC AGCGGCAGGG GGGAGGCGGC GGGCCCCAGC 
GGGGAGCCAC GGGACAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812833.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811135.2 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

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CloneID	OBa222787
Clone ID Related Accession (Same CDS sequence)	XM_010812833.3
Accession Version	XM_010812833.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010812833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACAGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811135.2
CDS	345..3971
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812833.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812833.3

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACAGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005216457.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216514.2 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa222788
Clone ID Related Accession (Same CDS sequence)	XM_005216457.4
Accession Version	XM_005216457.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216457.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACAGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216514.2
CDS	344..3880
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_005216457.4
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216457.4

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACAGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_024975266.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831034.1 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

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CloneID	OBa222789
Clone ID Related Accession (Same CDS sequence)	XM_024975266.1
Accession Version	XM_024975266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGAC CGAGCCCTTC CACCCCCCGG AGCAGGCCTC GTCAGCGCCG 
CACGACCCGG GCCTCCTGAG CCGGCCGTCT GCCTTCAGTA CGGTCCAGAG CAGCACCGCC 
GGCAACACGC TCCGCAACCT CAGCCTGGGT CCCCCGCGGC GCTCCCTGGG AGGCCCTCTG 
TCTGGCCACC CTTCCAGGTA CCACCGGGAC ATAGCCTCCG GGCTGACGGG ATCTGAGTGG 
ACCCGGACAG TGCTCAGTCT GAACTCTCGC TCCGAGGCAG AGTCCATGCC CCCGCCCAGG 
ACCAGCGCCT CTTCGGTGAG TCTACTCTCT GTGCTGCGAC AGCAGGAAGG TGGCTCTCAA 
GCCTCTGTGT ACACTTCAGC CACAGAAGGG AGGGGTTTTC CAGCCTCGGG GTTGGCAGCT 
GAGTCAGATG GAGGGAACGG CTCCAGCCAA AACAACTCGG GCAGCATCCG CCACGAGCTT 
CAGTGTGACC TGAGACGCTT CTTTCTGGAG TATGACCGGC TTCAGGAGCT GGACCAGAGC 
CTCAGCGGGG AGGCCCCCCA GGCCCAGCAG GCCCAGGAAA TGCTCAACAA TAACCTTGAA 
TCCGAACGGC CAGGGCCGTC CCACCAGCCC ACCCCGCACA GCAGCGAGAA CAACTCCAAC 
CTGTCCCGTG GCCACTTGAA CCGCTGCCGC GCTTGCCACA ATCTGCTGAC CTTCAACAAC 
GACACTCTGC GCTGGGAAAG AAGCACACCT AACTACTCCT CTGGCGAGGC CAGCGCCTCC 
TGGCAGGTCC CCGGCACCTT TGAGGGCATG GCCGCGAGTG GCAGCCAGCT GCCCCCTCTC 
GAGCGGACTG AGGGCCAGAC GGCCAGCTCC AGCAGGCTGG AGTTGGGCAA CTCTGCCAGC 
CCACAGGAGG AGAGGACTGT GGGGGTGGCC TTCAACCAGG AGACGGGCCA CTGGGAACGC 
ATTTACACCC AGGCCAGCCG ATCTGGAACT GTGTCACAGG AGGCCTTACA TCAGGATCTG 
CCGGAGGAGA GCTCTGAGGA GGATTCTCTC AGGAGATCTG CTTTAAAGAA GAGTCTGGCA 
GGGAGCCGGA GACCCGCTCC TGGGACAGCC AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACAGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831034.1
CDS	345..3878
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRSALKKSLAGSRRPAPGTARRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDSGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_024975266.1
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975266.1

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGAC CGAGCCCTTC CACCCCCCGG AGCAGGCCTC GTCAGCGCCG 
CACGACCCGG GCCTCCTGAG CCGGCCGTCT GCCTTCAGTA CGGTCCAGAG CAGCACCGCC 
GGCAACACGC TCCGCAACCT CAGCCTGGGT CCCCCGCGGC GCTCCCTGGG AGGCCCTCTG 
TCTGGCCACC CTTCCAGGTA CCACCGGGAC ATAGCCTCCG GGCTGACGGG ATCTGAGTGG 
ACCCGGACAG TGCTCAGTCT GAACTCTCGC TCCGAGGCAG AGTCCATGCC CCCGCCCAGG 
ACCAGCGCCT CTTCGGTGAG TCTACTCTCT GTGCTGCGAC AGCAGGAAGG TGGCTCTCAA 
GCCTCTGTGT ACACTTCAGC CACAGAAGGG AGGGGTTTTC CAGCCTCGGG GTTGGCAGCT 
GAGTCAGATG GAGGGAACGG CTCCAGCCAA AACAACTCGG GCAGCATCCG CCACGAGCTT 
CAGTGTGACC TGAGACGCTT CTTTCTGGAG TATGACCGGC TTCAGGAGCT GGACCAGAGC 
CTCAGCGGGG AGGCCCCCCA GGCCCAGCAG GCCCAGGAAA TGCTCAACAA TAACCTTGAA 
TCCGAACGGC CAGGGCCGTC CCACCAGCCC ACCCCGCACA GCAGCGAGAA CAACTCCAAC 
CTGTCCCGTG GCCACTTGAA CCGCTGCCGC GCTTGCCACA ATCTGCTGAC CTTCAACAAC 
GACACTCTGC GCTGGGAAAG AAGCACACCT AACTACTCCT CTGGCGAGGC CAGCGCCTCC 
TGGCAGGTCC CCGGCACCTT TGAGGGCATG GCCGCGAGTG GCAGCCAGCT GCCCCCTCTC 
GAGCGGACTG AGGGCCAGAC GGCCAGCTCC AGCAGGCTGG AGTTGGGCAA CTCTGCCAGC 
CCACAGGAGG AGAGGACTGT GGGGGTGGCC TTCAACCAGG AGACGGGCCA CTGGGAACGC 
ATTTACACCC AGGCCAGCCG ATCTGGAACT GTGTCACAGG AGGCCTTACA TCAGGATCTG 
CCGGAGGAGA GCTCTGAGGA GGATTCTCTC AGGAGATCTG CTTTAAAGAA GAGTCTGGCA 
GGGAGCCGGA GACCCGCTCC TGGGACAGCC AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACAGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001034522.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029694.2 Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), mRNA.

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CloneID	OBa40457
Clone ID Related Accession (Same CDS sequence)	NM_001034522.2
Accession Version	NM_001034522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC105220.1, NKLS02000015.1, DV890190.1, BF042604.1 and DV913753.1. On Feb 14, 2012 this sequence version replaced NM_001034522.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721694.3460.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC AACCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GGTACGTGAT GGTGGGCTTG GCCTCTCGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGTGG TGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ATCCCACCAC ATCGGTAACG TCTCAGGGCA CCCAAACTCT GGCCCTGCAG 
CTGCAAAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACGGCCGTGG TACAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AATATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GCGCACGCCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG CTGACAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCAGCCCGC TACCCCTGGG CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC 
GCCCCTCCTC CCGCTCACCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGACGGCGGG GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029694.2
CDS	317..3940
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	NM_001034522.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy and beclin 1 regulator 1 (AMBRA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034522.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA TGCCCAGAGA 
ATGATCCAGT ACCTGTCACG GAGAGACAGT ATCCGCCAGC GCTCCATGCG CTACCAGCAG 
AACCGCCTCC GTTCCTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC AACCGTGGAG 
GGAACCGACT TGGAGTTTGA GGACTTTGAG GACAGTGGTG ACAGATCCAG GCACCGAGCT 
CCTCGCAATG CCCGCATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAAT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TTGGTGATGG ACAGTCTGCC 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCGG AAATCAGTAA TGCCTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCGTT CATCCCTAGC 
AGTCAGAGGG GCTTTCCTGA CGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAACG CCATCTCAGT GAGCCTGTCC 
CCGATGGGCC GGTACGTGAT GGTGGGCTTG GCCTCTCGGA GGATCCTGCT GCACCCCTCC 
ACGGAGCACA TGGTGGCCCA GGTCTTCCGG CTGCAGCAGG CGCACGGTGG TGAGACCTCC 
ATGCGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCTG ACCAGCGGAG ACATGTCAGC 
ATCAATTCTG CCCGGTGGCT GCCCGAGCCA GGGCTTGGCC TGGCCTACGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCGGAGGCC TCAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTTCAC TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA 
ACCAGCAGAG CCACGTGGAG GACAGACAGA GACATGGGCC TGATGAATGC CATCGGCCTG 
CAGCCCCGGA ATCCCACCAC ATCGGTAACG TCTCAGGGCA CCCAAACTCT GGCCCTGCAG 
CTGCAAAATG CGGAGACCCA GACGGAGCGG GAGATACAGG AGCCGGGGGT GGCGGCTTCA 
GGTCCTGGTG AAGGGGAGGG CTCGGACTAC GGGGCCAGCG GGGAAGACGC GCTCAGCAGG 
ATCCAGCGGC TGATGGCTGA AGGGGGCATG ACGGCCGTGG TACAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGAAAC AATATCATTG TCAGCCACCG CATTCACCGC 
AGCTCCCAGA CGGGCACGGA GCCGGGGGCC GCGCACGCCC CCTCGCCGCA GCCCTCCACC 
TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG CTGACAGAGC GAGGCTTGAG CCCCCGGACA 
GCCTCCTGGG AGCAGCCCGC TACCCCTGGG CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC 
GCCCCTCCTC CCGCTCACCT CCCCAGCACC GAGGGACCAA CCCCGCCGCG CTGCGACCTG 
ACCAACAGCA ACCACCTTCC TGACGGCGGG GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC 
AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005216457.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216514.1 Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

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CloneID	OBa26746
Clone ID Related Accession (Same CDS sequence)	XM_005216457.3
Accession Version	XM_005216457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216457.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACGGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216514.1
CDS	395..3931
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_005216457.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216457.3

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGACCGA GCCCTTCCAC 
CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC GACCCGGGCC TCCTGAGCCG GCCGTCTGCC 
TTCAGTACGG TCCAGAGCAG CACCGCCGGC AACACGCTCC GCAACCTCAG CCTGGGTCCC 
CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT GGCCACCCTT CCAGGTACCA CCGGGACATA 
GCCTCCGGGC TGACGGGATC TGAGTGGACC CGGACAGTGC TCAGTCTGAA CTCTCGCTCC 
GAGGCAGAGT CCATGCCCCC GCCCAGGACC AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG 
CTGCGACAGC AGGAAGGTGG CTCTCAAGCC TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG TCAGATGGAG GGAACGGCTC CAGCCAAAAC 
AACTCGGGCA GCATCCGCCA CGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA CCAGAGCCTC AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC 
CAGGAAATGC TCAACAATAA CCTTGAATCC GAACGGCCAG GGCCGTCCCA CCAGCCCACC 
CCGCACAGCA GCGAGAACAA CTCCAACCTG TCCCGTGGCC ACTTGAACCG CTGCCGCGCT 
TGCCACAATC TGCTGACCTT CAACAACGAC ACTCTGCGCT GGGAAAGAAG CACACCTAAC 
TACTCCTCTG GCGAGGCCAG CGCCTCCTGG CAGGTCCCCG GCACCTTTGA GGGCATGGCC 
GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG CGGACTGAGG GCCAGACGGC CAGCTCCAGC 
AGGCTGGAGT TGGGCAACTC TGCCAGCCCA CAGGAGGAGA GGACTGTGGG GGTGGCCTTC 
AACCAGGAGA CGGGCCACTG GGAACGCATT TACACCCAGG CCAGCCGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATCTGCCG GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACGGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005216458.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216515.1 Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

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CloneID	OBa26747
Clone ID Related Accession (Same CDS sequence)	XM_005216458.3
Accession Version	XM_005216458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216458.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGAC CGAGCCCTTC CACCCCCCGG AGCAGGCCTC GTCAGCGCCG 
CACGACCCGG GCCTCCTGAG CCGGCCGTCT GCCTTCAGTA CGGTCCAGAG CAGCACCGCC 
GGCAACACGC TCCGCAACCT CAGCCTGGGT CCCCCGCGGC GCTCCCTGGG AGGCCCTCTG 
TCTGGCCACC CTTCCAGGTA CCACCGGGAC ATAGCCTCCG GGCTGACGGG ATCTGAGTGG 
ACCCGGACAG TGCTCAGTCT GAACTCTCGC TCCGAGGCAG AGTCCATGCC CCCGCCCAGG 
ACCAGCGCCT CTTCGGTGAG TCTACTCTCT GTGCTGCGAC AGCAGGAAGG TGGCTCTCAA 
GCCTCTGTGT ACACTTCAGC CACAGAAGGG AGGGGTTTTC CAGCCTCGGG GTTGGCAGCT 
GAGTCAGATG GAGGGAACGG CTCCAGCCAA AACAACTCGG GCAGCATCCG CCACGAGCTT 
CAGTGTGACC TGAGACGCTT CTTTCTGGAG TATGACCGGC TTCAGGAGCT GGACCAGAGC 
CTCAGCGGGG AGGCCCCCCA GGCCCAGCAG GCCCAGGAAA TGCTCAACAA TAACCTTGAA 
TCCGAACGGC CAGGGCCGTC CCACCAGCCC ACCCCGCACA GCAGCGAGAA CAACTCCAAC 
CTGTCCCGTG GCCACTTGAA CCGCTGCCGC GCTTGCCACA ATCTGCTGAC CTTCAACAAC 
GACACTCTGC GCTGGGAAAG AAGCACACCT AACTACTCCT CTGGCGAGGC CAGCGCCTCC 
TGGCAGGTCC CCGGCACCTT TGAGGGCATG GCCGCGAGTG GCAGCCAGCT GCCCCCTCTC 
GAGCGGACTG AGGGCCAGAC GGCCAGCTCC AGCAGGCTGG AGTTGGGCAA CTCTGCCAGC 
CCACAGGAGG AGAGGACTGT GGGGGTGGCC TTCAACCAGG AGACGGGCCA CTGGGAACGC 
ATTTACACCC AGGCCAGCCG ATCTGGAACT GTGTCACAGG AGGCCTTACA TCAGGATCTG 
CCGGAGGAGA GCTCTGAGGA GGATTCTCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACGGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216515.1
CDS	395..3871
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_005216458.3
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216458.3

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGAC CGAGCCCTTC CACCCCCCGG AGCAGGCCTC GTCAGCGCCG 
CACGACCCGG GCCTCCTGAG CCGGCCGTCT GCCTTCAGTA CGGTCCAGAG CAGCACCGCC 
GGCAACACGC TCCGCAACCT CAGCCTGGGT CCCCCGCGGC GCTCCCTGGG AGGCCCTCTG 
TCTGGCCACC CTTCCAGGTA CCACCGGGAC ATAGCCTCCG GGCTGACGGG ATCTGAGTGG 
ACCCGGACAG TGCTCAGTCT GAACTCTCGC TCCGAGGCAG AGTCCATGCC CCCGCCCAGG 
ACCAGCGCCT CTTCGGTGAG TCTACTCTCT GTGCTGCGAC AGCAGGAAGG TGGCTCTCAA 
GCCTCTGTGT ACACTTCAGC CACAGAAGGG AGGGGTTTTC CAGCCTCGGG GTTGGCAGCT 
GAGTCAGATG GAGGGAACGG CTCCAGCCAA AACAACTCGG GCAGCATCCG CCACGAGCTT 
CAGTGTGACC TGAGACGCTT CTTTCTGGAG TATGACCGGC TTCAGGAGCT GGACCAGAGC 
CTCAGCGGGG AGGCCCCCCA GGCCCAGCAG GCCCAGGAAA TGCTCAACAA TAACCTTGAA 
TCCGAACGGC CAGGGCCGTC CCACCAGCCC ACCCCGCACA GCAGCGAGAA CAACTCCAAC 
CTGTCCCGTG GCCACTTGAA CCGCTGCCGC GCTTGCCACA ATCTGCTGAC CTTCAACAAC 
GACACTCTGC GCTGGGAAAG AAGCACACCT AACTACTCCT CTGGCGAGGC CAGCGCCTCC 
TGGCAGGTCC CCGGCACCTT TGAGGGCATG GCCGCGAGTG GCAGCCAGCT GCCCCCTCTC 
GAGCGGACTG AGGGCCAGAC GGCCAGCTCC AGCAGGCTGG AGTTGGGCAA CTCTGCCAGC 
CCACAGGAGG AGAGGACTGT GGGGGTGGCC TTCAACCAGG AGACGGGCCA CTGGGAACGC 
ATTTACACCC AGGCCAGCCG ATCTGGAACT GTGTCACAGG AGGCCTTACA TCAGGATCTG 
CCGGAGGAGA GCTCTGAGGA GGATTCTCTC AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTATGCCCAG AGAATGATCC AGTACCTGTC ACGGAGAGAC 
AGTATCCGCC AGCGCTCCAT GCGCTACCAG CAGAACCGCC TCCGTTCCTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCAACCGTG GAGGGAACCG ACTTGGAGTT TGAGGACTTT 
GAGGACAGTG GTGACAGATC CAGGCACCGA GCTCCTCGCA ATGCCCGCAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AATACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATTGGTGA TGGACAGTCT GCCGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CGGAAATCAG TAATGCCTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC GTTCATCCCT AGCAGTCAGA GGGGCTTTCC TGACGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ACGCCATCTC AGTGAGCCTG TCCCCGATGG GCCGGTACGT GATGGTGGGC 
TTGGCCTCTC GGAGGATCCT GCTGCACCCC TCCACGGAGC ACATGGTGGC CCAGGTCTTC 
CGGCTGCAGC AGGCGCACGG TGGTGAGACC TCCATGCGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CTGACCAGCG GAGACATGTC AGCATCAATT CTGCCCGGTG GCTGCCCGAG 
CCAGGGCTTG GCCTGGCCTA CGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCGGAG 
GCCTCAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTT 
CACTCCAGCA GCCGAAGCAG CGAGAGGCCT GGAACCAGCA GAGCCACGTG GAGGACAGAC 
AGAGACATGG GCCTGATGAA TGCCATCGGC CTGCAGCCCC GGAATCCCAC CACATCGGTA 
ACGTCTCAGG GCACCCAAAC TCTGGCCCTG CAGCTGCAAA ATGCGGAGAC CCAGACGGAG 
CGGGAGATAC AGGAGCCGGG GGTGGCGGCT TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC 
TACGGGGCCA GCGGGGAAGA CGCGCTCAGC AGGATCCAGC GGCTGATGGC TGAAGGGGGC 
ATGACGGCCG TGGTACAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGA 
AACAATATCA TTGTCAGCCA CCGCATTCAC CGCAGCTCCC AGACGGGCAC GGAGCCGGGG 
GCCGCGCACG CCCCCTCGCC GCAGCCCTCC ACCTCTCGGG AACTGCTCCC GGAAGCCGGG 
CAGCTGACAG AGCGAGGCTT GAGCCCCCGG ACAGCCTCCT GGGAGCAGCC CGCTACCCCT 
GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC 
ACCGAGGGAC CAACCCCGCC GCGCTGCGAC CTGACCAACA GCAACCACCT TCCTGACGGC 
GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812831.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811133.1 Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa49664
Clone ID Related Accession (Same CDS sequence)	XM_010812831.2
Accession Version	XM_010812831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACGGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811133.1
CDS	399..4292
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812831.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812831.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGAGGCTGC TGGAGTCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA TGCCCAGAGA ATGATCCAGT ACCTGTCACG GAGAGACAGT 
ATCCGCCAGC GCTCCATGCG CTACCAGCAG AACCGCCTCC GTTCCTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC AACCGTGGAG GGAACCGACT TGGAGTTTGA GGACTTTGAG 
GACAGTGGTG ACAGATCCAG GCACCGAGCT CCTCGCAATG CCCGCATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAAT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TTGGTGATGG ACAGTCTGCC GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCGG AAATCAGTAA TGCCTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCGTT CATCCCTAGC AGTCAGAGGG GCTTTCCTGA CGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAACG CCATCTCAGT GAGCCTGTCC CCGATGGGCC GGTACGTGAT GGTGGGCTTG 
GCCTCTCGGA GGATCCTGCT GCACCCCTCC ACGGAGCACA TGGTGGCCCA GGTCTTCCGG 
CTGCAGCAGG CGCACGGTGG TGAGACCTCC ATGCGGAGAG TTTTCAACGT CCTTTACCCC 
ATGCCTGCTG ACCAGCGGAG ACATGTCAGC ATCAATTCTG CCCGGTGGCT GCCCGAGCCA 
GGGCTTGGCC TGGCCTACGG CACCAACAAA GGAGACCTGG TGATCTGCCG ACCGGAGGCC 
TCAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTTCAC 
TCCAGCAGCC GAAGCAGCGA GAGGCCTGGA ACCAGCAGAG CCACGTGGAG GACAGACAGA 
GACATGGGCC TGATGAATGC CATCGGCCTG CAGCCCCGGA ATCCCACCAC ATCGGTAACG 
TCTCAGGGCA CCCAAACTCT GGCCCTGCAG CTGCAAAATG CGGAGACCCA GACGGAGCGG 
GAGATACAGG AGCCGGGGGT GGCGGCTTCA GGTCCTGGTG AAGGGGAGGG CTCGGACTAC 
GGGGCCAGCG GGGAAGACGC GCTCAGCAGG ATCCAGCGGC TGATGGCTGA AGGGGGCATG 
ACGGCCGTGG TACAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGAAAC 
AATATCATTG TCAGCCACCG CATTCACCGC AGCTCCCAGA CGGGCACGGA GCCGGGGGCC 
GCGCACGCCC CCTCGCCGCA GCCCTCCACC TCTCGGGAAC TGCTCCCGGA AGCCGGGCAG 
CTGACAGAGC GAGGCTTGAG CCCCCGGACA GCCTCCTGGG AGCAGCCCGC TACCCCTGGG 
CGGGAGCCCG CCCTGCCCTC CTCCTCCCCC GCCCCTCCTC CCGCTCACCT CCCCAGCACC 
GAGGGACCAA CCCCGCCGCG CTGCGACCTG ACCAACAGCA ACCACCTTCC TGACGGCGGG 
GGCAGCGGCA GGGGGGAGGC GGCGGGCCCC AGCGGGGAGC CACGGGACAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812832.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811134.1 Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

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CloneID	OBa49665
Clone ID Related Accession (Same CDS sequence)	XM_010812832.2
Accession Version	XM_010812832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCAACCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACGGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811134.1
CDS	400..4206
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPTVEGTDLEFEDFEDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812832.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812832.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGATTATCTC CTGCTGCATA CTATGCCCAG 
AGAATGATCC AGTACCTGTC ACGGAGAGAC AGTATCCGCC AGCGCTCCAT GCGCTACCAG 
CAGAACCGCC TCCGTTCCTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCAACCGTG 
GAGGGAACCG ACTTGGAGTT TGAGGACTTT GAGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACGGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_010812833.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010811135.1 Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

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CloneID	OBa49666
Clone ID Related Accession (Same CDS sequence)	XM_010812833.2
Accession Version	XM_010812833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACGGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010811135.1
CDS	401..4027
Translation	MKVVPEKNAVRILWGRERGTRTFGAQRLLQELVEDKTRCMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATASEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPVDGAELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSCIQVGEQSTVQDPATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSAPHDPGLLSRPSAFSTVQSSTAGNTLRNLSLGPPRRSLGGPLSGHPSRYHRDIASGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLAAESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQAQQAQEMLNNNLESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERSTPNYSSGEASASWQVPGTFEGMAASGSQLPPLERTEGQTASSSRLELGNSASPQEERTVGVAFNQETGHWERIYTQASRSGTVSQEALHQDLPEESSEEDSLRRDSGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEASNSGVEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREIQEPGVAASGPGEGEGSDYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHAPSPQPSTSRELLPEAGQLTERGLSPRTASWEQPATPGREPALPSSSPAPPPAHLPSTEGPTPPRCDLTNSNHLPDGGGSGRGEAAGPSGEPRDR

Target ORF information:

RefSeq Version	XM_010812833.2
Organism	Bos taurus(cattle)
Definition	Bos taurus autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010812833.2

ORF Insert Sequence:

ATGAAGGTTG TTCCAGAGAA GAATGCCGTC CGCATTCTCT GGGGGAGAGA ACGGGGCACT 
CGGACATTTG GAGCTCAGCG ACTTCTGCAG GAGCTGGTTG AAGATAAAAC CCGGTGTATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCCACCTT TTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCTTCC ACTCACGTAA ACCATAACAT CTATATCACG 
GAAGTGAAGA CGGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGCAC GCCGTGGTGT 
GTCACGTTTC ATCCCACCAT CTCAGGCCTC ATCGCTTCTG GCTGCCTAGA TGGGGAGGTT 
CGGATCTGGG ATTTACACGG TGGCAGTGAG AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC CACGGCTCAG CTTCTGCTGA TTGCCACTGC CAGCGAGATC 
CACTTCTGGG ACTGGAGTCG CCGGGAACCC TTTGCCGTGG TGAAGACAGC TAGCGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCT CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGACGAA CCAGAGATCC CCGTAGACGG AGCAGAACTG 
TCGCACTACC GTCAGCGGGC CCTCCTGCAG TCACAGCCCG TGCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCCTCTTGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATCCTGCCAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCCGGCTC CGACAGCGGG TCAGTTACCC CACAGCCGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCGGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGGGCTAC TACCCCTTCC 
TTTTCTTTTG TGCAGACCGA GCCCTTCCAC CCCCCGGAGC AGGCCTCGTC AGCGCCGCAC 
GACCCGGGCC TCCTGAGCCG GCCGTCTGCC TTCAGTACGG TCCAGAGCAG CACCGCCGGC 
AACACGCTCC GCAACCTCAG CCTGGGTCCC CCGCGGCGCT CCCTGGGAGG CCCTCTGTCT 
GGCCACCCTT CCAGGTACCA CCGGGACATA GCCTCCGGGC TGACGGGATC TGAGTGGACC 
CGGACAGTGC TCAGTCTGAA CTCTCGCTCC GAGGCAGAGT CCATGCCCCC GCCCAGGACC 
AGCGCCTCTT CGGTGAGTCT ACTCTCTGTG CTGCGACAGC AGGAAGGTGG CTCTCAAGCC 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCAG CCTCGGGGTT GGCAGCTGAG 
TCAGATGGAG GGAACGGCTC CAGCCAAAAC AACTCGGGCA GCATCCGCCA CGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA CCAGAGCCTC 
AGCGGGGAGG CCCCCCAGGC CCAGCAGGCC CAGGAAATGC TCAACAATAA CCTTGAATCC 
GAACGGCCAG GGCCGTCCCA CCAGCCCACC CCGCACAGCA GCGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACTTGAACCG CTGCCGCGCT TGCCACAATC TGCTGACCTT CAACAACGAC 
ACTCTGCGCT GGGAAAGAAG CACACCTAAC TACTCCTCTG GCGAGGCCAG CGCCTCCTGG 
CAGGTCCCCG GCACCTTTGA GGGCATGGCC GCGAGTGGCA GCCAGCTGCC CCCTCTCGAG 
CGGACTGAGG GCCAGACGGC CAGCTCCAGC AGGCTGGAGT TGGGCAACTC TGCCAGCCCA 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTC AACCAGGAGA CGGGCCACTG GGAACGCATT 
TACACCCAGG CCAGCCGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATCTGCCG 
GAGGAGAGCT CTGAGGAGGA TTCTCTCAGG AGGGACAGTG GTGACAGATC CAGGCACCGA 
GCTCCTCGCA ATGCCCGCAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AATACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATTGGTGA TGGACAGTCT 
GCCGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CGGAAATCAG TAATGCCTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC GTTCATCCCT 
AGCAGTCAGA GGGGCTTTCC TGACGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ACGCCATCTC AGTGAGCCTG 
TCCCCGATGG GCCGGTACGT GATGGTGGGC TTGGCCTCTC GGAGGATCCT GCTGCACCCC 
TCCACGGAGC ACATGGTGGC CCAGGTCTTC CGGCTGCAGC AGGCGCACGG TGGTGAGACC 
TCCATGCGGA GAGTTTTCAA CGTCCTTTAC CCCATGCCTG CTGACCAGCG GAGACATGTC 
AGCATCAATT CTGCCCGGTG GCTGCCCGAG CCAGGGCTTG GCCTGGCCTA CGGCACCAAC 
AAAGGAGACC TGGTGATCTG CCGACCGGAG GCCTCAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTT CACTCCAGCA GCCGAAGCAG CGAGAGGCCT 
GGAACCAGCA GAGCCACGTG GAGGACAGAC AGAGACATGG GCCTGATGAA TGCCATCGGC 
CTGCAGCCCC GGAATCCCAC CACATCGGTA ACGTCTCAGG GCACCCAAAC TCTGGCCCTG 
CAGCTGCAAA ATGCGGAGAC CCAGACGGAG CGGGAGATAC AGGAGCCGGG GGTGGCGGCT 
TCAGGTCCTG GTGAAGGGGA GGGCTCGGAC TACGGGGCCA GCGGGGAAGA CGCGCTCAGC 
AGGATCCAGC GGCTGATGGC TGAAGGGGGC ATGACGGCCG TGGTACAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGA AACAATATCA TTGTCAGCCA CCGCATTCAC 
CGCAGCTCCC AGACGGGCAC GGAGCCGGGG GCCGCGCACG CCCCCTCGCC GCAGCCCTCC 
ACCTCTCGGG AACTGCTCCC GGAAGCCGGG CAGCTGACAG AGCGAGGCTT GAGCCCCCGG 
ACAGCCTCCT GGGAGCAGCC CGCTACCCCT GGGCGGGAGC CCGCCCTGCC CTCCTCCTCC 
CCCGCCCCTC CTCCCGCTCA CCTCCCCAGC ACCGAGGGAC CAACCCCGCC GCGCTGCGAC 
CTGACCAACA GCAACCACCT TCCTGACGGC GGGGGCAGCG GCAGGGGGGA GGCGGCGGGC 
CCCAGCGGGG AGCCACGGGA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AMBRA1
Entrez Gene ID	517263
Full Name	autophagy and beclin 1 regulator 1
General protein information	Preferred Names autophagy and beclin 1 regulator 1 Names activating molecule in BECN1-regulated autophagy protein 1 activating molecule in beclin-1-regulated autophagy
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AMBRA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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