AMBRA1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005253014.2	XP_005253071.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
NM_001267783.1	NP_001254712.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 3
NM_001367471.1	NP_001354400.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 2
NM_001367468.1	NP_001354397.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 5
NM_001300731.1	NP_001287660.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 4
XM_005253014.3	XP_005253071.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_006718260.2	XP_006718323.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
NM_001267782.1	NP_001254711.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
NM_001267782.2	NP_001254711.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
NM_001300731.2	NP_001287660.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 4
XM_005253009.4	XP_005253066.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_024448605.1	XP_024304373.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_017018007.1	XP_016873496.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_017018008.1	XP_016873497.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
XM_005253011.4	XP_005253068.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_005253009.3	XP_005253066.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
NM_001267783.2	NP_001254712.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 3
XM_006718259.2	XP_006718322.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_005253011.3	XP_005253068.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
XM_024448606.1	XP_024304374.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
NM_001367470.1	NP_001354399.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 7
NM_001367469.1	NP_001354398.1	activating molecule in BECN1-regulated autophagy protein 1 isoform 6
NM_017749.3	NP_060219.2	activating molecule in BECN1-regulated autophagy protein 1 isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu69547	XM_024448606.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu107382	NM_001367470.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu92368	XM_017018007.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92369	XM_017018008.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu09585	NM_001367471.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu09585	NM_017749.3 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu09779	NM_001267783.2 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu12050	NM_001267782.2 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44914	NM_001367468.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44916	NM_001300731.2 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44917	NM_001367469.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu09585	XM_005253014.3 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu44914	XM_005253009.4 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44914	XM_006718259.2 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44914	XM_024448605.1 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44915	XM_005253011.4 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44917	XM_006718260.2 Latest version!	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu09585	XM_005253014.2	Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
OHu12050	NM_001267782.1	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44916	NM_001300731.1	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44914	XM_005253009.3	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu09779	NM_001267783.1	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu44915	XM_005253011.3	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

AMBRA1 ( XM_024448606.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304374.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu69547
Clone ID Related Accession (Same CDS sequence)	XM_024448606.1
Accession Version	XM_024448606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGGTCT TGGTTGCCCC TTACGTCCAA AGGCACCAGA TCACTCAGCA AATGTCCCTG 
AATTCAGTGA TTGGTGGAAG GGAAGACACC AGCTTTTTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304374.1
CDS	349..3153
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRVLVAPYVQRHQITQQMSLNSVIGGREDTSFFG

Target ORF information:

RefSeq Version	XM_024448606.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448606.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGGTCT TGGTTGCCCC TTACGTCCAA AGGCACCAGA TCACTCAGCA AATGTCCCTG 
AATTCAGTGA TTGGTGGAAG GGAAGACACC AGCTTTTTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001367470.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354399.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu107382
Clone ID Related Accession (Same CDS sequence)	NM_001367470.1
Accession Version	NM_001367470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.176312.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACGGG TCCGTCTGGT GAGATTTGAT CCACTTGGAC ACTACTTACT CACAGCAATT 
GTTAACCCCT CTAATCAACA GGGTGATGAC GAACCAGAGA TCCCCATAGA TGGAACAGAA 
TTATCCCACT ACCGTCAGCG TGCCCTCCTG CAATCACAGC CAGTTCGCCG GACGCCTCTC 
CTCCACAATT TCCTGCACAT GCTGTCCTCC CGCTCTTCTG GCATCCAGAC CGAGCCCTTC 
CATCCCCCGG AGCAGGCCTC GTCAACGCAG CAGGACCAGG GCCTCCTGAA CCGGCCGTCT 
GCCTTCAGTA CAGTCCAGAG CAGCACTGCC GGCAACACGC TCCGCAACCT CAGTCTGGGT 
CCTACCCGCC GCTCTTTGGG AGGGCCTCTG TCTAGCCACC CTTCTAGGTA TCACCGAGAA 
ATAGCTCCTG GGTTGACAGG ATCTGAGTGG ACCCGGACAG TACTCAGTCT GAACTCCCGC 
TCTGAGGCGG AATCCATGCC CCCGCCCAGA ACCAGTGCCT CTTCGGTGAG TTTGCTGTCT 
GTGCTGAGAC AGCAGGAAGG TGGCTCTCAG GCATCTGTGT ACACTTCAGC CACAGAAGGG 
AGGGGTTTTC CGGCATCAGG GTTGGCAACT GAGTCAGATG GAGGGAATGG CTCCAGCCAA 
AACAACTCGG GCAGCATTCG CCATGAGCTT CAGTGTGACC TGAGACGCTT CTTTCTGGAG 
TATGACCGGC TTCAGGAGCT GGATCAGAGC CTGAGTGGGG AAGCTCCCCA GACCCAACAG 
GCCCAGGAAA TGCTCAACAA TAACATTGAA TCTGAGAGGC CAGGCCCTTC CCACCAGCCC 
ACCCCACACA GCAGTGAGAA CAACTCCAAC CTGTCCCGTG GCCACCTGAA TCGCTGTCGT 
GCTTGCCACA ATCTCCTGAC CTTCAACAAC GATACCCTGC GCTGGGAAAG AACCACACCT 
AACTACTCCT CTGGCGAGGC TAGTTCCTCT TGGCAGGTCC CCAGCTCCTT TGAGAGTGTG 
CCATCAAGTG GCAGCCAGTT GCCACCTCTC GAGCGGACTG AGGGCCAAAC GCCCAGCTCC 
AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT CCGCAGGAGG AGAGGACTGT GGGGGTGGCC 
TTTAACCAGG AGACAGGCCA CTGGGAAAGA ATTTACACCC AGTCCAGCAG ATCTGGAACT 
GTGTCACAGG AGGCCTTACA TCAGGATATG CCTGAGGAGA GCTCTGAGGA GGATTCACTC 
AGGAGGAGGC TGCTGGAATC TTCCCTCATT TCATTATCCC GTTATGATGG AGCAGGATCC 
AGAGAGCACC CAATTTACCC AGACCCAGCG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354399.1
CDS	843..3932
Translation	MERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001367470.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367470.1

ORF Insert Sequence:

ATGGAACGGG TCCGTCTGGT GAGATTTGAT CCACTTGGAC ACTACTTACT CACAGCAATT 
GTTAACCCCT CTAATCAACA GGGTGATGAC GAACCAGAGA TCCCCATAGA TGGAACAGAA 
TTATCCCACT ACCGTCAGCG TGCCCTCCTG CAATCACAGC CAGTTCGCCG GACGCCTCTC 
CTCCACAATT TCCTGCACAT GCTGTCCTCC CGCTCTTCTG GCATCCAGAC CGAGCCCTTC 
CATCCCCCGG AGCAGGCCTC GTCAACGCAG CAGGACCAGG GCCTCCTGAA CCGGCCGTCT 
GCCTTCAGTA CAGTCCAGAG CAGCACTGCC GGCAACACGC TCCGCAACCT CAGTCTGGGT 
CCTACCCGCC GCTCTTTGGG AGGGCCTCTG TCTAGCCACC CTTCTAGGTA TCACCGAGAA 
ATAGCTCCTG GGTTGACAGG ATCTGAGTGG ACCCGGACAG TACTCAGTCT GAACTCCCGC 
TCTGAGGCGG AATCCATGCC CCCGCCCAGA ACCAGTGCCT CTTCGGTGAG TTTGCTGTCT 
GTGCTGAGAC AGCAGGAAGG TGGCTCTCAG GCATCTGTGT ACACTTCAGC CACAGAAGGG 
AGGGGTTTTC CGGCATCAGG GTTGGCAACT GAGTCAGATG GAGGGAATGG CTCCAGCCAA 
AACAACTCGG GCAGCATTCG CCATGAGCTT CAGTGTGACC TGAGACGCTT CTTTCTGGAG 
TATGACCGGC TTCAGGAGCT GGATCAGAGC CTGAGTGGGG AAGCTCCCCA GACCCAACAG 
GCCCAGGAAA TGCTCAACAA TAACATTGAA TCTGAGAGGC CAGGCCCTTC CCACCAGCCC 
ACCCCACACA GCAGTGAGAA CAACTCCAAC CTGTCCCGTG GCCACCTGAA TCGCTGTCGT 
GCTTGCCACA ATCTCCTGAC CTTCAACAAC GATACCCTGC GCTGGGAAAG AACCACACCT 
AACTACTCCT CTGGCGAGGC TAGTTCCTCT TGGCAGGTCC CCAGCTCCTT TGAGAGTGTG 
CCATCAAGTG GCAGCCAGTT GCCACCTCTC GAGCGGACTG AGGGCCAAAC GCCCAGCTCC 
AGCAGGCTGG AGTTGAGCAG CTCTGCTAGT CCGCAGGAGG AGAGGACTGT GGGGGTGGCC 
TTTAACCAGG AGACAGGCCA CTGGGAAAGA ATTTACACCC AGTCCAGCAG ATCTGGAACT 
GTGTCACAGG AGGCCTTACA TCAGGATATG CCTGAGGAGA GCTCTGAGGA GGATTCACTC 
AGGAGGAGGC TGCTGGAATC TTCCCTCATT TCATTATCCC GTTATGATGG AGCAGGATCC 
AGAGAGCACC CAATTTACCC AGACCCAGCG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_017018007.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873496.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

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CloneID	OHu92368
Clone ID Related Accession (Same CDS sequence)	XM_017018007.1
Accession Version	XM_017018007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873496.1
CDS	359..3988
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_017018007.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018007.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_017018008.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873497.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu92369
Clone ID Related Accession (Same CDS sequence)	XM_017018008.1
Accession Version	XM_017018008.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGGTCT TGGTTGCCCC TTACGTCCAA 
AGGCACCAGA TCACTCAGCA AATGTCCCTG AATTCAGTGA TTGGTGGAAG GGAAGACACC 
AGCTTTTTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873497.1
CDS	359..3433
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRVLVAPYVQRHQITQQMSLNSVIGGREDTSFFG

Target ORF information:

RefSeq Version	XM_017018008.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018008.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGGTCT TGGTTGCCCC TTACGTCCAA 
AGGCACCAGA TCACTCAGCA AATGTCCCTG AATTCAGTGA TTGGTGGAAG GGAAGACACC 
AGCTTTTTTG GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001367471.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354400.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 8, mRNA.

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CloneID	OHu09585
Clone ID Related Accession (Same CDS sequence)	XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
Accession Version	NM_001367471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. On Dec 1, 2018 this sequence version replaced XM_005253014.4. Transcript Variant: This variant (8), as well as variant 2, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.197066.1, SRR1660807.82204.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354400.1
CDS	317..3943
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001367471.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367471.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_017749.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060219.2 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 2, mRNA.

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CloneID	OHu09585
Clone ID Related Accession (Same CDS sequence)	XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
Accession Version	NM_017749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC045609.1, DB232750.1, AK023197.1 and BF589843.1. On Jun 20, 2012 this sequence version replaced NM_017749.2. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Variants 2 and 8 both encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045609.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060219.2
CDS	361..3987
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_017749.3
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017749.3

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001267783.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254712.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

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CloneID	OHu09779
Clone ID Related Accession (Same CDS sequence)	NM_001267783.1 , NM_001267783.2
Accession Version	NM_001267783.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AL834190.1, DQ870924.1 and BF589843.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.69365.1, SRR1660805.26538.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254712.1
CDS	363..3902
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001267783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267783.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001267783.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254712.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

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CloneID	OHu09779
Clone ID Related Accession (Same CDS sequence)	NM_001267783.1 , NM_001267783.2
Accession Version	NM_001267783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AL834190.1 and DQ870924.1. On Jun 2, 2019 this sequence version replaced NM_001267783.1. Transcript Variant: This variant (3) lacks two consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL834190.1, SRR1660805.69365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254712.1
CDS	317..3856
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001267783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267783.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC ACGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAACCGTC TCCGTTCTTC CACCTCCTCC 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001267782.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254711.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12050
Clone ID Related Accession (Same CDS sequence)	NM_001267782.1 , NM_001267782.2
Accession Version	NM_001267782.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AK000301.1, BC045609.1, AC115097.6 and BF589843.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC 
AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT 
ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC 
CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC 
CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC 
CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC 
CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG 
AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC 
TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG 
GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT 
GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT 
GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG 
GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT 
ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT 
GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT 
TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT 
GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC 
AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG 
GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG 
GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC 
CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG 
CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA 
CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC 
ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG 
ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC 
TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG 
ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT 
TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG 
GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC 
TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA 
CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG 
GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT 
ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT 
GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC 
CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254711.1
CDS	363..4268
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRSLALSPRLEYSGAILAHCKLRLPGSCHSPASASQVAGTTGAHHHARLIFAFLVEMEFHHVSQAGLELLTSGDLPTSASQSAGITGVSHRAWPRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001267782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267782.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC 
AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT 
ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC 
CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC 
CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC 
CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC 
CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG 
AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC 
TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG 
GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT 
GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT 
GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG 
GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT 
ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT 
GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT 
TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT 
GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC 
AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG 
GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG 
GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC 
CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG 
CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA 
CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC 
ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG 
ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC 
TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG 
ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT 
TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG 
GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC 
TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA 
CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG 
GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT 
ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT 
GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC 
CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001267782.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254711.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu12050
Clone ID Related Accession (Same CDS sequence)	NM_001267782.1 , NM_001267782.2
Accession Version	NM_001267782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AK000301.1, BC045609.1 and AC115097.6. On Jun 3, 2019 this sequence version replaced NM_001267782.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC 
AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT 
ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC 
CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC 
CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC 
CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC 
CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG 
AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC 
TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG 
GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT 
GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT 
GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG 
GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT 
ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT 
GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT 
TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT 
GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC 
AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG 
GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG 
GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC 
CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG 
CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA 
CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC 
ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG 
ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC 
TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG 
ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT 
TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG 
GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC 
TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA 
CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG 
GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT 
ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT 
GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC 
CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254711.1
CDS	317..4222
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRSLALSPRLEYSGAILAHCKLRLPGSCHSPASASQVAGTTGAHHHARLIFAFLVEMEFHHVSQAGLELLTSGDLPTSASQSAGITGVSHRAWPRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001267782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267782.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGACGGAGTC TTGCTCTGTC ACCTAGGCTG GAGTACAGCG GCGCTATCTT GGCTCACTGC 
AAGCTCCGCC TCCCAGGTTC ATGCCATTCT CCTGCCTCAG CTTCCCAAGT AGCTGGGACT 
ACGGGAGCCC ACCACCACGC CCGGCTAATT TTTGCATTTT TAGTAGAGAT GGAGTTTCAC 
CATGTTAGCC AGGCTGGTCT CGAACTCCTG ACCTCAGGTG ATCTGCCCAC CTCGGCCTCC 
CAAAGTGCTG GGATTACAGG CGTGAGCCAC CGCGCCTGGC CGAGGCTGCT GGAATCTTCC 
CTCATTTCAT TATCCCGTTA TGATGGAGCA GGATCCAGAG AGCACCCAAT TTACCCAGAC 
CCAGCGAGAT TATCTCCTGC TGCATACTAC GCCCAGAGGA TGATCCAGTA TCTCTCACGG 
AGAGACAGTA TTCGCCAGCG CTCCATGCGC TACCAACAGA ACCGTCTCCG TTCTTCCACC 
TCCTCCTCTT CCTCAGACAA CCAGGGTCCA TCAGTAGAGG GAACCGACTT GGAATTTGAG 
GACTTTGAGG ACAATGGTGA CAGATCCAGG CACCGAGCTC CACGCAATGC CCGGATGTCT 
GCACCTTCGC TTGGACGCTT TGTCCCAAGG CGTTTCTTGC TGCCTGAGTA CTTGCCTTAT 
GCTGGGATTT TTCATGAACG TGGACAGCCT GGCTTGGCTA CTCACTCTTC TGTTAACAGG 
GTCCTGGCAG GGGCAGTGAT CGGTGATGGA CAGTCTGCTG TGGCCAGTAA CATTGCCAAT 
ACTACCTACC GGCTCCAGTG GTGGGACTTC ACTAAGTTTG ACCTCCCTGA AATCAGTAAT 
GCTTCCGTGA ATGTGCTGGT GCAGAACTGC AAGATCTACA ATGATGCCAG CTGTGACATT 
TCTGCAGATG GCCAGCTCCT GGCAGCTTTC ATCCCCAGCA GCCAGAGGGG CTTTCCTGAT 
GAAGGCATCC TGGCAGTGTA CTCCCTGGCC CCCCATAACC TGGGCGAAAT GCTCTACACC 
AAGCGATTTG GTCCCAATGC CATTTCGGTG AGCCTGTCCC CAATGGGCAG ATATGTAATG 
GTGGGCTTGG CCTCACGAAG GATCCTGCTG CACCCCTCCA CAGAGCACAT GGTGGCCCAG 
GTCTTCAGGC TGCAACAGGC CCATGGTGGA GAGACCTCCA TGAGGAGAGT TTTCAACGTC 
CTTTATCCCA TGCCTGCCGA CCAGCGGAGA CATGTCAGTA TCAACTCTGC CCGTTGGCTG 
CCTGAGCCAG GGCTTGGCTT GGCCTATGGT ACTAACAAAG GAGACCTGGT GATCTGCCGA 
CCAGAGGCCT TAAACTCTGG TGTTGAGTAC TACTGGGACC AGCTGAACGA GACGGTCTTC 
ACTGTCCATT CCAACAGCAG GAGCAGCGAG CGGCCTGGAA CCAGCAGAGC CACATGGAGG 
ACAGACAGAG ACATGGGGCT GATGAATGCC ATTGGGCTTC AGCCCCGGAA CCCTGCCACC 
TCAGTGACAT CTCAGGGCAC CCAGACTCTG GCCCTTCAGC TGCAGAATGC CGAAACACAG 
ACTGAGAGGG AGGTGCCGGA GCCAGGGACA GCCGCCTCAG GTCCTGGTGA AGGTGAGGGT 
TCAGAGTATG GTGCCAGTGG AGAAGATGCG CTCAGCAGGA TCCAGAGGCT GATGGCGGAG 
GGCGGCATGA CAGCCGTGGT GCAGCGGGAG CAGAGCACCA CCATGGCCTC CATGGGCGGC 
TTCGGCAACA ACATCATCGT CAGCCACCGC ATTCACCGCA GCTCTCAGAC GGGCACTGAA 
CCTGGTGCCG CCCACACCTC CTCACCCCAG CCCTCCACCT CTCGGGGACT GCTCCCAGAG 
GCCGGGCAAC TGGCAGAGCG AGGCCTAAGC CCCCGGACAG CTTCCTGGGA CCAGCCTGGT 
ACCCCTGGGC GGGAGCCAAC CCAGCCAACC CTGCCCTCTT CCTCCCCTGT CCCCATTCCT 
GTTTCCCTTC CCAGCGCTGA GGGACCAACC CTCCACTGCG AGTTGACCAA TAACAACCAC 
CTTCTGGATG GTGGCAGCAG CAGGGGGGAC GCTGCAGGCC CTAGGGGAGA ACCACGGAAC 
AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001367468.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354397.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 5, mRNA.

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CloneID	OHu44914
Clone ID Related Accession (Same CDS sequence)	NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
Accession Version	NM_001367468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK302808.1, AC127035.6 and AC116021.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302808.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354397.1
CDS	361..4257
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001367468.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367468.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001300731.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287660.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu44916
Clone ID Related Accession (Same CDS sequence)	NM_001300731.1 , NM_001300731.2
Accession Version	NM_001300731.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1, AB051523.1 and BF589843.1. On Jul 11, 2014 this sequence version replaced XM_005253012.2. Transcript Variant: This variant (4) retains an alternate in-frame intron and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) contains an alternate internal segment and lacks two other alternate internal segments compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287660.1
CDS	363..4079
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001300731.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300731.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001300731.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287660.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu44916
Clone ID Related Accession (Same CDS sequence)	NM_001300731.1 , NM_001300731.2
Accession Version	NM_001300731.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB070055.1 and AB051523.1. On Jun 2, 2019 this sequence version replaced NM_001300731.1. Transcript Variant: This variant (4) retains an alternate in-frame intron and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (4) contains an alternate internal segment and lacks two other alternate internal segments compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB051523.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287660.1
CDS	317..4033
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001300731.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300731.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( NM_001367469.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354398.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 6, mRNA.

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CloneID	OHu44917
Clone ID Related Accession (Same CDS sequence)	XM_006718260.2 , NM_001367469.1
Accession Version	NM_001367469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC127035.6, AC115097.6, AC024293.18 and AC116021.5. On Dec 1, 2018 this sequence version replaced XM_006718260.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.151930.1, SRR1660809.19725.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354398.1
CDS	317..3676
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	NM_001367469.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367469.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253014.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253071.1 Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

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CloneID	OHu09585
Clone ID Related Accession (Same CDS sequence)	XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
Accession Version	XM_005253014.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005253071.1

Translation

MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253014.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens autophagy/beclin-1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253014.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253014.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253071.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

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CloneID	OHu09585
Clone ID Related Accession (Same CDS sequence)	XM_005253014.2 , NM_001367471.1 , XM_005253014.3 , NM_017749.3
Accession Version	XM_005253014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253071.1
CDS	359..3985
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253014.3
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253014.3

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGAGGCTGC TGGAATCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCACG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AACCGTCTCC GTTCTTCCAC CTCCTCCTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCCAG GCACCGAGCT 
CCACGCAATG CCCGGATGTC TGCACCTTCG CTTGGACGCT TTGTCCCAAG GCGTTTCTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAAC GTGGACAGCC TGGCTTGGCT 
ACTCACTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTGA TCGGTGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC CGGCTCCAGT GGTGGGACTT CACTAAGTTT 
GACCTCCCTG AAATCAGTAA TGCTTCCGTG AATGTGCTGG TGCAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCCAGC 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ACTCCCTGGC CCCCCATAAC 
CTGGGCGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCGGT GAGCCTGTCC 
CCAATGGGCA GATATGTAAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCC 
ACAGAGCACA TGGTGGCCCA GGTCTTCAGG CTGCAACAGG CCCATGGTGG AGAGACCTCC 
ATGAGGAGAG TTTTCAACGT CCTTTATCCC ATGCCTGCCG ACCAGCGGAG ACATGTCAGT 
ATCAACTCTG CCCGTTGGCT GCCTGAGCCA GGGCTTGGCT TGGCCTATGG TACTAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GTGTTGAGTA CTACTGGGAC 
CAGCTGAACG AGACGGTCTT CACTGTCCAT TCCAACAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGAG GACAGACAGA GACATGGGGC TGATGAATGC CATTGGGCTT 
CAGCCCCGGA ACCCTGCCAC CTCAGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTTCAG 
CTGCAGAATG CCGAAACACA GACTGAGAGG GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA 
GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT GGTGCCAGTG GAGAAGATGC GCTCAGCAGG 
ATCCAGAGGC TGATGGCGGA GGGCGGCATG ACAGCCGTGG TGCAGCGGGA GCAGAGCACC 
ACCATGGCCT CCATGGGCGG CTTCGGCAAC AACATCATCG TCAGCCACCG CATTCACCGC 
AGCTCTCAGA CGGGCACTGA ACCTGGTGCC GCCCACACCT CCTCACCCCA GCCCTCCACC 
TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA CTGGCAGAGC GAGGCCTAAG CCCCCGGACA 
GCTTCCTGGG ACCAGCCTGG TACCCCTGGG CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT 
TCCTCCCCTG TCCCCATTCC TGTTTCCCTT CCCAGCGCTG AGGGACCAAC CCTCCACTGC 
GAGTTGACCA ATAACAACCA CCTTCTGGAT GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC 
CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253009.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253066.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu44914
Clone ID Related Accession (Same CDS sequence)	NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
Accession Version	XM_005253009.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253009.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253066.1
CDS	349..4245
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253009.4
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253009.4

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_024448605.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304373.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu44914
Clone ID Related Accession (Same CDS sequence)	NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
Accession Version	XM_024448605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304373.1
CDS	713..4609
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_024448605.1
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448605.1

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253009.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253066.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

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CloneID	OHu44914
Clone ID Related Accession (Same CDS sequence)	NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
Accession Version	XM_005253009.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253066.1
CDS	359..4255
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253009.3
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253009.3

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006718259.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718322.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

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CloneID	OHu44914
Clone ID Related Accession (Same CDS sequence)	NM_001367468.1 , XM_005253009.4 , XM_024448605.1 , XM_005253009.3 , XM_006718259.2
Accession Version	XM_006718259.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718322.1
CDS	240..4136
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_006718259.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718259.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGGAGGCTGC TGGAATCTTC CCTCATTTCA 
TTATCCCGTT ATGATGGAGC AGGATCCAGA GAGCACCCAA TTTACCCAGA CCCAGCGAGA 
TTATCTCCTG CTGCATACTA CGCCCAGAGG ATGATCCAGT ATCTCTCACG GAGAGACAGT 
ATTCGCCAGC GCTCCATGCG CTACCAACAG AACCGTCTCC GTTCTTCCAC CTCCTCCTCT 
TCCTCAGACA ACCAGGGTCC ATCAGTAGAG GGAACCGACT TGGAATTTGA GGACTTTGAG 
GACAATGGTG ACAGATCCAG GCACCGAGCT CCACGCAATG CCCGGATGTC TGCACCTTCG 
CTTGGACGCT TTGTCCCAAG GCGTTTCTTG CTGCCTGAGT ACTTGCCTTA TGCTGGGATT 
TTTCATGAAC GTGGACAGCC TGGCTTGGCT ACTCACTCTT CTGTTAACAG GGTCCTGGCA 
GGGGCAGTGA TCGGTGATGG ACAGTCTGCT GTGGCCAGTA ACATTGCCAA TACTACCTAC 
CGGCTCCAGT GGTGGGACTT CACTAAGTTT GACCTCCCTG AAATCAGTAA TGCTTCCGTG 
AATGTGCTGG TGCAGAACTG CAAGATCTAC AATGATGCCA GCTGTGACAT TTCTGCAGAT 
GGCCAGCTCC TGGCAGCTTT CATCCCCAGC AGCCAGAGGG GCTTTCCTGA TGAAGGCATC 
CTGGCAGTGT ACTCCCTGGC CCCCCATAAC CTGGGCGAAA TGCTCTACAC CAAGCGATTT 
GGTCCCAATG CCATTTCGGT GAGCCTGTCC CCAATGGGCA GATATGTAAT GGTGGGCTTG 
GCCTCACGAA GGATCCTGCT GCACCCCTCC ACAGAGCACA TGGTGGCCCA GGTCTTCAGG 
CTGCAACAGG CCCATGGTGG AGAGACCTCC ATGAGGAGAG TTTTCAACGT CCTTTATCCC 
ATGCCTGCCG ACCAGCGGAG ACATGTCAGT ATCAACTCTG CCCGTTGGCT GCCTGAGCCA 
GGGCTTGGCT TGGCCTATGG TACTAACAAA GGAGACCTGG TGATCTGCCG ACCAGAGGCC 
TTAAACTCTG GTGTTGAGTA CTACTGGGAC CAGCTGAACG AGACGGTCTT CACTGTCCAT 
TCCAACAGCA GGAGCAGCGA GCGGCCTGGA ACCAGCAGAG CCACATGGAG GACAGACAGA 
GACATGGGGC TGATGAATGC CATTGGGCTT CAGCCCCGGA ACCCTGCCAC CTCAGTGACA 
TCTCAGGGCA CCCAGACTCT GGCCCTTCAG CTGCAGAATG CCGAAACACA GACTGAGAGG 
GAGGTGCCGG AGCCAGGGAC AGCCGCCTCA GGTCCTGGTG AAGGTGAGGG TTCAGAGTAT 
GGTGCCAGTG GAGAAGATGC GCTCAGCAGG ATCCAGAGGC TGATGGCGGA GGGCGGCATG 
ACAGCCGTGG TGCAGCGGGA GCAGAGCACC ACCATGGCCT CCATGGGCGG CTTCGGCAAC 
AACATCATCG TCAGCCACCG CATTCACCGC AGCTCTCAGA CGGGCACTGA ACCTGGTGCC 
GCCCACACCT CCTCACCCCA GCCCTCCACC TCTCGGGGAC TGCTCCCAGA GGCCGGGCAA 
CTGGCAGAGC GAGGCCTAAG CCCCCGGACA GCTTCCTGGG ACCAGCCTGG TACCCCTGGG 
CGGGAGCCAA CCCAGCCAAC CCTGCCCTCT TCCTCCCCTG TCCCCATTCC TGTTTCCCTT 
CCCAGCGCTG AGGGACCAAC CCTCCACTGC GAGTTGACCA ATAACAACCA CCTTCTGGAT 
GGTGGCAGCA GCAGGGGGGA CGCTGCAGGC CCTAGGGGAG AACCACGGAA CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253011.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253068.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu44915
Clone ID Related Accession (Same CDS sequence)	XM_005253011.4 , XM_005253011.3
Accession Version	XM_005253011.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253011.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253068.1
CDS	349..4158
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253011.4
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253011.4

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_005253011.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253068.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

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CloneID	OHu44915
Clone ID Related Accession (Same CDS sequence)	XM_005253011.4 , XM_005253011.3
Accession Version	XM_005253011.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253068.1
CDS	359..4168
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_005253011.3
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253011.3

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTGCAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGCCCCAGGA CTTCCGCTTA CATCAGGCTC CGACAGCGGG TCAGTTACCC CACAGCTGAG 
TGCTGCCAGC ACCTTGGGAT CCTGTGCCTT TGCAGCCGCT GCTCTGGCAC TCGAGTTCCT 
TCCCTCTTGC CACACCAGGA CAGTGTCCCC CCTGCTTCTG CCAGAGCTAC TACCCCTTCC 
TTTTCTTTTG TACAGACCGA GCCCTTCCAT CCCCCGGAGC AGGCCTCGTC AACGCAGCAG 
GACCAGGGCC TCCTGAACCG GCCGTCTGCC TTCAGTACAG TCCAGAGCAG CACTGCCGGC 
AACACGCTCC GCAACCTCAG TCTGGGTCCT ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT 
AGCCACCCTT CTAGGTATCA CCGAGAAATA GCTCCTGGGT TGACAGGATC TGAGTGGACC 
CGGACAGTAC TCAGTCTGAA CTCCCGCTCT GAGGCGGAAT CCATGCCCCC GCCCAGAACC 
AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG CTGAGACAGC AGGAAGGTGG CTCTCAGGCA 
TCTGTGTACA CTTCAGCCAC AGAAGGGAGG GGTTTTCCGG CATCAGGGTT GGCAACTGAG 
TCAGATGGAG GGAATGGCTC CAGCCAAAAC AACTCGGGCA GCATTCGCCA TGAGCTTCAG 
TGTGACCTGA GACGCTTCTT TCTGGAGTAT GACCGGCTTC AGGAGCTGGA TCAGAGCCTG 
AGTGGGGAAG CTCCCCAGAC CCAACAGGCC CAGGAAATGC TCAACAATAA CATTGAATCT 
GAGAGGCCAG GCCCTTCCCA CCAGCCCACC CCACACAGCA GTGAGAACAA CTCCAACCTG 
TCCCGTGGCC ACCTGAATCG CTGTCGTGCT TGCCACAATC TCCTGACCTT CAACAACGAT 
ACCCTGCGCT GGGAAAGAAC CACACCTAAC TACTCCTCTG GCGAGGCTAG TTCCTCTTGG 
CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG 
CGGACTGAGG GCCAAACGCC CAGCTCCAGC AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG 
CAGGAGGAGA GGACTGTGGG GGTGGCCTTT AACCAGGAGA CAGGCCACTG GGAAAGAATT 
TACACCCAGT CCAGCAGATC TGGAACTGTG TCACAGGAGG CCTTACATCA GGATATGCCT 
GAGGAGAGCT CTGAGGAGGA TTCACTCAGG AGATTATCTC CTGCTGCATA CTACGCCCAG 
AGGATGATCC AGTATCTCTC ACGGAGAGAC AGTATTCGCC AGCGCTCCAT GCGCTACCAA 
CAGAACCGTC TCCGTTCTTC CACCTCCTCC TCTTCCTCAG ACAACCAGGG TCCATCAGTA 
GAGGGAACCG ACTTGGAATT TGAGGACTTT GAGGACAATG GTGACAGATC CAGGCACCGA 
GCTCCACGCA ATGCCCGGAT GTCTGCACCT TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC 
TTGCTGCCTG AGTACTTGCC TTATGCTGGG ATTTTTCATG AACGTGGACA GCCTGGCTTG 
GCTACTCACT CTTCTGTTAA CAGGGTCCTG GCAGGGGCAG TGATCGGTGA TGGACAGTCT 
GCTGTGGCCA GTAACATTGC CAATACTACC TACCGGCTCC AGTGGTGGGA CTTCACTAAG 
TTTGACCTCC CTGAAATCAG TAATGCTTCC GTGAATGTGC TGGTGCAGAA CTGCAAGATC 
TACAATGATG CCAGCTGTGA CATTTCTGCA GATGGCCAGC TCCTGGCAGC TTTCATCCCC 
AGCAGCCAGA GGGGCTTTCC TGATGAAGGC ATCCTGGCAG TGTACTCCCT GGCCCCCCAT 
AACCTGGGCG AAATGCTCTA CACCAAGCGA TTTGGTCCCA ATGCCATTTC GGTGAGCCTG 
TCCCCAATGG GCAGATATGT AATGGTGGGC TTGGCCTCAC GAAGGATCCT GCTGCACCCC 
TCCACAGAGC ACATGGTGGC CCAGGTCTTC AGGCTGCAAC AGGCCCATGG TGGAGAGACC 
TCCATGAGGA GAGTTTTCAA CGTCCTTTAT CCCATGCCTG CCGACCAGCG GAGACATGTC 
AGTATCAACT CTGCCCGTTG GCTGCCTGAG CCAGGGCTTG GCTTGGCCTA TGGTACTAAC 
AAAGGAGACC TGGTGATCTG CCGACCAGAG GCCTTAAACT CTGGTGTTGA GTACTACTGG 
GACCAGCTGA ACGAGACGGT CTTCACTGTC CATTCCAACA GCAGGAGCAG CGAGCGGCCT 
GGAACCAGCA GAGCCACATG GAGGACAGAC AGAGACATGG GGCTGATGAA TGCCATTGGG 
CTTCAGCCCC GGAACCCTGC CACCTCAGTG ACATCTCAGG GCACCCAGAC TCTGGCCCTT 
CAGCTGCAGA ATGCCGAAAC ACAGACTGAG AGGGAGGTGC CGGAGCCAGG GACAGCCGCC 
TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG TATGGTGCCA GTGGAGAAGA TGCGCTCAGC 
AGGATCCAGA GGCTGATGGC GGAGGGCGGC ATGACAGCCG TGGTGCAGCG GGAGCAGAGC 
ACCACCATGG CCTCCATGGG CGGCTTCGGC AACAACATCA TCGTCAGCCA CCGCATTCAC 
CGCAGCTCTC AGACGGGCAC TGAACCTGGT GCCGCCCACA CCTCCTCACC CCAGCCCTCC 
ACCTCTCGGG GACTGCTCCC AGAGGCCGGG CAACTGGCAG AGCGAGGCCT AAGCCCCCGG 
ACAGCTTCCT GGGACCAGCC TGGTACCCCT GGGCGGGAGC CAACCCAGCC AACCCTGCCC 
TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC CTTCCCAGCG CTGAGGGACC AACCCTCCAC 
TGCGAGTTGA CCAATAACAA CCACCTTCTG GATGGTGGCA GCAGCAGGGG GGACGCTGCA 
GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AMBRA1 ( XM_006718260.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718323.1 Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

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CloneID	OHu44917
Clone ID Related Accession (Same CDS sequence)	XM_006718260.2 , NM_001367469.1
Accession Version	XM_006718260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718323.1
CDS	359..3718
Translation	MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Target ORF information:

RefSeq Version	XM_006718260.2
Organism	Homo sapiens(human)
Definition	Homo sapiens autophagy and beclin 1 regulator 1 (AMBRA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718260.2

ORF Insert Sequence:

ATGAAGGTTG TCCCAGAAAA GAATGCTGTC CGGATACTCT GGGGGCGAGA ACGGGGTGCT 
CGGGCCATGG GAGCTCAGCG GCTTCTGCAG GAGCTGGTAG AAGATAAAAC CCGGTGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTGCCG GATAGTCCAC GCTCTACCTT CTTATTGGCC 
TTCAGCCCAG ACAGGACTCT CTTAGCCTCC ACCCATGTGA ACCATAATAT CTATATTACG 
GAGGTGAAGA CTGGCAAGTG TGTTCATTCC CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACCAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAGGTT 
AGGATTTGGG ATTTACACGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATT 
GCCTCCCTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TTGCCACTGC CAATGAGATC 
CACTTCTGGG ACTGGAGTCG ACGGGAACCC TTTGCTGTGG TGAAGACAGC TAGTGAGATG 
GAACGGGTCC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTCAC AGCAATTGTT 
AACCCCTCTA ATCAACAGGG TGATGACGAA CCAGAGATCC CCATAGATGG AACAGAATTA 
TCCCACTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC GCCTCTCCTC 
CACAATTTCC TGCACATGCT GTCCTCCCGC TCTTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCCTCGTC AACGCAGCAG GACCAGGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCCGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGCCGCT CTTTGGGAGG GCCTCTGTCT AGCCACCCTT CTAGGTATCA CCGAGAAATA 
GCTCCTGGGT TGACAGGATC TGAGTGGACC CGGACAGTAC TCAGTCTGAA CTCCCGCTCT 
GAGGCGGAAT CCATGCCCCC GCCCAGAACC AGTGCCTCTT CGGTGAGTTT GCTGTCTGTG 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTACA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCGG CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGCTC CAGCCAAAAC 
AACTCGGGCA GCATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTCTT TCTGGAGTAT 
GACCGGCTTC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAG CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA CATTGAATCT GAGAGGCCAG GCCCTTCCCA CCAGCCCACC 
CCACACAGCA GTGAGAACAA CTCCAACCTG TCCCGTGGCC ACCTGAATCG CTGTCGTGCT 
TGCCACAATC TCCTGACCTT CAACAACGAT ACCCTGCGCT GGGAAAGAAC CACACCTAAC 
TACTCCTCTG GCGAGGCTAG TTCCTCTTGG CAGGTCCCCA GCTCCTTTGA GAGTGTGCCA 
TCAAGTGGCA GCCAGTTGCC ACCTCTCGAG CGGACTGAGG GCCAAACGCC CAGCTCCAGC 
AGGCTGGAGT TGAGCAGCTC TGCTAGTCCG CAGGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CAGGCCACTG GGAAAGAATT TACACCCAGT CCAGCAGATC TGGAACTGTG 
TCACAGGAGG CCTTACATCA GGATATGCCT GAGGAGAGCT CTGAGGAGGA TTCACTCAGG 
AGGGACAATG GTGACAGATC CAGGCACCGA GCTCCACGCA ATGCCCGGAT GTCTGCACCT 
TCGCTTGGAC GCTTTGTCCC AAGGCGTTTC TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AACGTGGACA GCCTGGCTTG GCTACTCACT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TGATCGGTGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACCGGCTCC AGTGGTGGGA CTTCACTAAG TTTGACCTCC CTGAAATCAG TAATGCTTCC 
GTGAATGTGC TGGTGCAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCC AGCAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTACTCCCT GGCCCCCCAT AACCTGGGCG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC GGTGAGCCTG TCCCCAATGG GCAGATATGT AATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCCACAGAGC ACATGGTGGC CCAGGTCTTC 
AGGCTGCAAC AGGCCCATGG TGGAGAGACC TCCATGAGGA GAGTTTTCAA CGTCCTTTAT 
CCCATGCCTG CCGACCAGCG GAGACATGTC AGTATCAACT CTGCCCGTTG GCTGCCTGAG 
CCAGGGCTTG GCTTGGCCTA TGGTACTAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGTGTTGA GTACTACTGG GACCAGCTGA ACGAGACGGT CTTCACTGTC 
CATTCCAACA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GAGGACAGAC 
AGAGACATGG GGCTGATGAA TGCCATTGGG CTTCAGCCCC GGAACCCTGC CACCTCAGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTT CAGCTGCAGA ATGCCGAAAC ACAGACTGAG 
AGGGAGGTGC CGGAGCCAGG GACAGCCGCC TCAGGTCCTG GTGAAGGTGA GGGTTCAGAG 
TATGGTGCCA GTGGAGAAGA TGCGCTCAGC AGGATCCAGA GGCTGATGGC GGAGGGCGGC 
ATGACAGCCG TGGTGCAGCG GGAGCAGAGC ACCACCATGG CCTCCATGGG CGGCTTCGGC 
AACAACATCA TCGTCAGCCA CCGCATTCAC CGCAGCTCTC AGACGGGCAC TGAACCTGGT 
GCCGCCCACA CCTCCTCACC CCAGCCCTCC ACCTCTCGGG GACTGCTCCC AGAGGCCGGG 
CAACTGGCAG AGCGAGGCCT AAGCCCCCGG ACAGCTTCCT GGGACCAGCC TGGTACCCCT 
GGGCGGGAGC CAACCCAGCC AACCCTGCCC TCTTCCTCCC CTGTCCCCAT TCCTGTTTCC 
CTTCCCAGCG CTGAGGGACC AACCCTCCAC TGCGAGTTGA CCAATAACAA CCACCTTCTG 
GATGGTGGCA GCAGCAGGGG GGACGCTGCA GGCCCTAGGG GAGAACCACG GAACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AMBRA1
Entrez Gene ID	55626
Full Name	autophagy and beclin 1 regulator 1
Synonyms	DCAF3,WDR94
General protein information	Preferred Names autophagy and beclin 1 regulator 1 Names activating molecule in BECN1-regulated autophagy protein 1 DDB1 and CUL4 associated factor 3 WD repeat domain 94 activating molecule in beclin-1-regulated autophagy autophagy/beclin-1 regulator 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p11.2
Disorder MIM:	611359

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AMBRA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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