Ambra1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ambra1
Entrez Gene ID	59319
Full Name	autophagy and beclin 1 regulator 1
Synonyms	Nyw1
General protein information	Preferred Names autophagy and beclin 1 regulator 1 Names activating molecule in BECN1-regulated autophagy protein 1 autophagy/beclin 1 regulator 1 ischemia related factor NYW-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001134341.2	NP_001127813.2	activating molecule in BECN1-regulated autophagy protein 1
XM_008762030.2	XP_008760252.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
XM_008762033.2	XP_008760255.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
XM_008762034.2	XP_008760256.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
NM_001134341.1	NP_001127813.1	activating molecule in BECN1-regulated autophagy protein 1
XM_008762032.2	XP_008760254.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
XM_008762031.2	XP_008760253.1	activating molecule in BECN1-regulated autophagy protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ambra1	NP_766257.3
Gallus gallus (chicken)	AMBRA1	XP_004941659.1
Danio rerio (zebrafish)	ambra1	XP_002667715.3
Canis lupus familiaris (dog)	AMBRA1	XP_005631347.1
Rattus norvegicus (Norway rat)	Ambra1	NP_001127813.1
Xenopus tropicalis (tropical clawed frog)	ambra1	XP_004913444.1
Homo sapiens (human)	AMBRA1	NP_060219.2
Pan troglodytes (chimpanzee)	AMBRA1	XP_508396.2
	AMBRA1	XP_001112256.1
Bos taurus (cattle)	AMBRA1	NP_001029694.2

Gene Ontology
Bibliography

Related articles in PubMed

AMBRA1, a novel ?-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.
Miki Y, Tanji K, Mori F, Tatara Y, Utsumi J, Sasaki H, Kakita A, Takahashi H, Fimia GM, Wakabayashi K
Brain pathology (Zurich, Switzerland)28(1)28-42(2018 Jan)

IRGM governs the core autophagy machinery to conduct antimicrobial defense.
Chauhan S, Mandell MA, Deretic V
Molecular cell58(3)507-21(2015 May)

AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1.
Strappazzon F, Nazio F, Corrado M, Cianfanelli V, Romagnoli A, Fimia GM, Campello S, Nardacci R, Piacentini M, Campanella M, Cecconi F
Cell death and differentiation22(3)419-32(2015 Mar)

Ambra1 modulates starvation-induced autophagy through AMPK signaling pathway in cardiomyocytes.
Shi C, Wu J, Fu M, Zhang B, Wang J, Yang X, Chi Y
Biochemical and biophysical research communications452(3)308-14(2014 Sep)

Functional interaction between autophagy and ciliogenesis.
Pampliega O, Orhon I, Patel B, Sridhar S, D?az-Carretero A, Beau I, Codogno P, Satir BH, Satir P, Cuervo AM
Nature502(7470)194-200(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ambra1 is a crucial regulator of autophagy and apoptosis through AMPK signaling pathway in cardiomyocytes that maintains the balance between autophagy and apoptosis.
Title: Ambra1 modulates starvation-induced autophagy through AMPK signaling pathway in cardiomyocytes.

The following Ambra1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ambra1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02625	NM_001134341.1 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43494	NM_001134341.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43494	XM_008762030.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43495	XM_008762031.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43496	XM_008762032.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43497	XM_008762033.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa43498	XM_008762034.2 Latest version!	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ambra1 ( NM_001134341.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001127813.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.

Price & Availability↑

CloneID	ORa02625
Clone ID Related Accession (Same CDS sequence)	NM_001134341.1
Accession Version	NM_001134341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAHX01023491.1, AAHX01023492.1, AAHX01023493.1, AAHX01023495.1, AAHX01023498.1 and AAHX01023499.1. On Aug 23, 2008 this sequence version replaced XM_001069366.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127813.1
CDS	366..4268
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	NM_001134341.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134341.1

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCAC GCTCGACCTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( NM_001134341.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001127813.2 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.

Price & Availability↑

CloneID	ORa43494
Clone ID Related Accession (Same CDS sequence)	NM_001134341.2 , XM_008762030.2
Accession Version	NM_001134341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07052882.1, AABR07052885.1, AABR07052887.1 and AABR07072897.1. On Jun 28, 2018 this sequence version replaced NM_001134341.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001127813.2
CDS	366..4268
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	NM_001134341.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001134341.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_008762030.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760252.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

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CloneID	ORa43494
Clone ID Related Accession (Same CDS sequence)	NM_001134341.2 , XM_008762030.2
Accession Version	XM_008762030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760252.1
CDS	349..4251
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	XM_008762030.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762030.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_008762031.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760253.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa43495
Clone ID Related Accession (Same CDS sequence)	XM_008762031.2
Accession Version	XM_008762031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCATCACTTG GACGCTTTGT CCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAGCGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACC 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCCGTGAATG TCCTGGTACA GAACTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AGCTCCTGGC AGCTTTCATC 
CCGAGTAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCAA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCTACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AGCAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAACGTCCTT TACCCCATGC CTGCGGACCA GCGGAGACAC 
GTCAGCATCA ACTCTGCCCG CTGGCTACCT GAGCCAGGGC TCGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT TGAGTACTAC 
TGGGACCAGC TAAATGAAAC GGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGCTTGAT GAATGCAATT 
GGGCTGCAAC CTCGGAACCC TACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGACAGCA 
TCCTCAGGTC CTGGTGAAGG CGAGGGCTCG GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCCGGATCC AGAGGCTAAT GGCCGAGGGT GGCATGACAG CTGTGGTACA GCGAGAACAG 
AGTACCACCA TGGCCTCCAT GGGTGGCTTC GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTACT CTCAGAACCT GGGCAACTGG CAGAGCGAGG ACTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGAAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATAGTAGCAA TGTGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACGGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760253.1
CDS	204..4019
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	XM_008762031.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762031.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGATTAT CTCCTGCTGC ATACTACGCC 
CAGAGGATGA TCCAGTATCT CTCAAGGAGA GACAGTATTC GCCAGCGCTC CATGCGCTAC 
CAACAGAATC GGCTTCGTTC TTCCACCTCC TCTTCTTCCT CAGACAACCA GGGTCCATCA 
GTAGAGGGAA CCGACTTGGA ATTTGAGGAC TTTGAGGACA ATGGTGACAG ATCTAGGCAC 
CGAGCTCCCC GAAATGCCCG AATGTCTGCA CCATCACTTG GACGCTTTGT CCCAAGGCGT 
TTTTTGCTGC CTGAGTACTT GCCTTATGCT GGGATTTTTC ATGAGCGTGG ACAGCCTGGC 
TTGGCTACTC ATTCTTCTGT TAACAGGGTC CTGGCAGGGG CAGTAATTGG AGATGGACAG 
TCTGCTGTGG CCAGTAACAT TGCCAATACT ACCTACAGGC TCCAGTGGTG GGACTTCACC 
AAGTTTGACC TTCCAGAAAT CAGTAATGCC TCCGTGAATG TCCTGGTACA GAACTGCAAG 
ATCTACAATG ATGCCAGCTG TGACATTTCT GCAGATGGCC AGCTCCTGGC AGCTTTCATC 
CCGAGTAGCC AGAGGGGCTT TCCTGATGAA GGCATCCTGG CAGTGTATTC CCTGGCCCCC 
CATAACTTGG GGGAAATGCT CTACACCAAG CGATTTGGTC CCAATGCCAT TTCTGTAAGC 
CTGTCCCCAA TGGGCCGATA CGTTATGGTG GGCTTGGCCT CACGAAGGAT CCTGCTGCAC 
CCCTCTACAG AGCACATGGT AGCCCAGGTC TTCAGGCTGC AGCAGGCCCA TGGTGGTGAG 
ACTTCCATGA GGAGAGTTTT CAACGTCCTT TACCCCATGC CTGCGGACCA GCGGAGACAC 
GTCAGCATCA ACTCTGCCCG CTGGCTACCT GAGCCAGGGC TCGGTTTGGC CTATGGCACC 
AACAAAGGAG ACCTGGTGAT CTGCCGACCA GAGGCCTTAA ACTCTGGCAT TGAGTACTAC 
TGGGACCAGC TAAATGAAAC GGTCTTCACT GTCCACTCAA GTAGCAGGAG CAGCGAGCGG 
CCTGGAACCA GCAGAGCCAC ATGGCGGACA GACAGAGACA TGGGCTTGAT GAATGCAATT 
GGGCTGCAAC CTCGGAACCC TACCACCTCT GTGACATCTC AGGGCACCCA GACTCTGGCC 
CTCCAGTTGC AGAATGCTGA AACACAGACT GAGAGGGAGG AAGAGGAACC AGGGACAGCA 
TCCTCAGGTC CTGGTGAAGG CGAGGGCTCG GAATATGGTG GCAGTGGGGA AGATGCCCTC 
AGCCGGATCC AGAGGCTAAT GGCCGAGGGT GGCATGACAG CTGTGGTACA GCGAGAACAG 
AGTACCACCA TGGCCTCCAT GGGTGGCTTC GGCAACAACA TCATCGTTAG CCACCGCATT 
CACCGCAGCT CCCAGACAGG CACAGAGTCA GGGGCTGCTC GTACCTCCTC ACCCCAGCCC 
TCCACCTCTC GAGGACTACT CTCAGAACCT GGGCAACTGG CAGAGCGAGG ACTAAGCCCC 
CGGACAGCCT CCTGGGACCA GCCTAGTACC TCTGGAAGGG AACTACCCCA GCCAGCCCTG 
TCCTCTTCCT CCCCTGTCCC CATTCCAGTC CCCCTTGCCA GCAATGAGGG ACCAACCATG 
CACTGCAATG TGACCAATAA CAGTCACCTT CCTGAGGGTG ATAGTAGCAA TGTGGGGGAA 
GCTGCAGGCC CTAGTGGGGA GCCACGGAAC CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_008762032.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760254.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa43496
Clone ID Related Accession (Same CDS sequence)	XM_008762032.2
Accession Version	XM_008762032.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760254.1
CDS	204..3926
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTTVECCQHPGILCLCSRCAGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	XM_008762032.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762032.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGGTGGG AGAGCAAAGC 
ACAGTACAAG ATTCTGCTAC CCCCTCACCC CCACCGCCTC CCCCTCAGCC CTCCACGGAG 
CGTCCCAGGA CTTCCGCTTA CATCAGACTC CGACAGCGGG TCAGTTACCC CACCACAGTT 
GAGTGCTGCC AGCACCCTGG GATCCTGTGC CTTTGCAGCC GTTGTGCTGG CACTCGAGTT 
CCTTCCCTCT TGCCACACCA GGACAGTGTC CCCCCTGCTT CTGCCAGAGC TACTACCCCT 
TCCTTTTCTT TTGTACAGAC CGAGCCCTTC CATCCCCCGG AGCAGGCTTC ATCAACGCAG 
CAGGACCAAG GCCTCCTGAA CCGGCCGTCT GCCTTCAGTA CAGTCCAGAG CAGCACTGCG 
GGCAACACGC TCCGCAACCT CAGTCTGGGT CCTACCCGTC GCTCTTTGGG GGGCCCACTG 
TCCAGCCACC CATCTAGGTA TCACAGAGAA CTAGCCCCTG GGCTGACAGG TTCTGAGTGG 
ACCCGGACAG TACTCACTCT GAACTCACGT TCTGAGGTAG AATCTATGCC CCCACCGAGG 
ACCAGTGCCT CCTCAGTGAG TTTGCTTTCT GTACTGAGAC AGCAGGAAGG TGGCTCTCAG 
GCATCTGTGT ATACTTCAGC CACAGAAGGG AGGGGTTTTC CATCATCAGG GTTGGCAACT 
GAGTCAGATG GAGGGAATGG TTCCAGCCAA AACAACTCAG GCAACATTCG CCATGAGCTT 
CAGTGTGACC TGAGACGCTT TTTTTTGGAG TATGACAGGC TGCAGGAGCT GGATCAGAGC 
CTGAGTGGGG AAACTCCCCA GACCCAACAG GCCCAGGAAA TGCTCAACAA TAATATTGAA 
TCTGAGAGAC CAGGTCCTTC CCATCAGCCT ACCCCACACA GCAGTGAGAA CAACTCCAAC 
CTTTCTCGGG GCCACCTTAA TCGATGCCGT GCTTGCCATA ATCTTCTAAC CTTCAACAAT 
GATACCCTTC GTTGGGAAAG AACCACACCT AACTACTCAT CCGGTGAGGC TAGCTCTTCC 
TGGCATGTCT CCACTACTTT TGAGGGCATG CCACCGAGTG GCAACCAACT ACCACCTCTT 
GAGCGGACTG AGGGCCAAAT GCCTAACTCC AGCAGGCTGG AGTTGAGCAG TTCTGCTAGT 
TCACAAGAGG AGAGGACTGT GGGGGTGGCC TTTAACCAGG AGACCGGCCA TTGGGAACGA 
ATTTATACCC AGTCCAGCAG ATCTGGAACT GTATCACAGG AGGCCTTACA TCAAGATATG 
CCTGAGGAAA GCTCTGAGGA AGACTCCCTG AGGAGGAGGC TGCTGGAGTC TTCCCTCATT 
TCATTATCCC GTTATGATGG AGCAGGATCC AGAGAGCACC CAATTTACCC AGACCCAGCG 
AGGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_008762033.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760255.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

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CloneID	ORa43497
Clone ID Related Accession (Same CDS sequence)	XM_008762033.2
Accession Version	XM_008762033.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAAGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGTCGCT CTTTGGGGGG CCCACTGTCC AGCCACCCAT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCACGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA ACATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTTTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA TCAGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTT TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCCG GTGAGGCTAG CTCTTCCTGG CATGTCTCCA CTACTTTTGA GGGCATGCCA 
CCGAGTGGCA ACCAACTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAACTCCAGC 
AGGCTGGAGT TGAGCAGTTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CCGGCCATTG GGAACGAATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAGGAAAGCT CTGAGGAAGA CTCCCTGAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCATCA CTTGGACGCT TTGTCCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAGC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACCAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCCGTG AATGTCCTGG TACAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCGAGT 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCAATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCT 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAGCAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCGG ACCAGCGGAG ACACGTCAGC 
ATCAACTCTG CCCGCTGGCT ACCTGAGCCA GGGCTCGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATTGAGTA CTACTGGGAC 
CAGCTAAATG AAACGGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGCT TGATGAATGC AATTGGGCTG 
CAACCTCGGA ACCCTACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGAC AGCATCCTCA 
GGTCCTGGTG AAGGCGAGGG CTCGGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCCGG 
ATCCAGAGGC TAATGGCCGA GGGTGGCATG ACAGCTGTGG TACAGCGAGA ACAGAGTACC 
ACCATGGCCT CCATGGGTGG CTTCGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TACTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGGACTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCTAG TACCTCTGGA AGGGAACTAC CCCAGCCAGC CCTGTCCTCT 
TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATAGTA GCAATGTGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACG GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760255.1
CDS	204..3833
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	XM_008762033.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762033.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAAGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGTCGCT CTTTGGGGGG CCCACTGTCC AGCCACCCAT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCACGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA ACATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTTTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA TCAGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTT TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCCG GTGAGGCTAG CTCTTCCTGG CATGTCTCCA CTACTTTTGA GGGCATGCCA 
CCGAGTGGCA ACCAACTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAACTCCAGC 
AGGCTGGAGT TGAGCAGTTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CCGGCCATTG GGAACGAATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAGGAAAGCT CTGAGGAAGA CTCCCTGAGG 
AGGAGGCTGC TGGAGTCTTC CCTCATTTCA TTATCCCGTT ATGATGGAGC AGGATCCAGA 
GAGCACCCAA TTTACCCAGA CCCAGCGAGA TTATCTCCTG CTGCATACTA CGCCCAGAGG 
ATGATCCAGT ATCTCTCAAG GAGAGACAGT ATTCGCCAGC GCTCCATGCG CTACCAACAG 
AATCGGCTTC GTTCTTCCAC CTCCTCTTCT TCCTCAGACA ACCAGGGTCC ATCAGTAGAG 
GGAACCGACT TGGAATTTGA GGACTTTGAG GACAATGGTG ACAGATCTAG GCACCGAGCT 
CCCCGAAATG CCCGAATGTC TGCACCATCA CTTGGACGCT TTGTCCCAAG GCGTTTTTTG 
CTGCCTGAGT ACTTGCCTTA TGCTGGGATT TTTCATGAGC GTGGACAGCC TGGCTTGGCT 
ACTCATTCTT CTGTTAACAG GGTCCTGGCA GGGGCAGTAA TTGGAGATGG ACAGTCTGCT 
GTGGCCAGTA ACATTGCCAA TACTACCTAC AGGCTCCAGT GGTGGGACTT CACCAAGTTT 
GACCTTCCAG AAATCAGTAA TGCCTCCGTG AATGTCCTGG TACAGAACTG CAAGATCTAC 
AATGATGCCA GCTGTGACAT TTCTGCAGAT GGCCAGCTCC TGGCAGCTTT CATCCCGAGT 
AGCCAGAGGG GCTTTCCTGA TGAAGGCATC CTGGCAGTGT ATTCCCTGGC CCCCCATAAC 
TTGGGGGAAA TGCTCTACAC CAAGCGATTT GGTCCCAATG CCATTTCTGT AAGCCTGTCC 
CCAATGGGCC GATACGTTAT GGTGGGCTTG GCCTCACGAA GGATCCTGCT GCACCCCTCT 
ACAGAGCACA TGGTAGCCCA GGTCTTCAGG CTGCAGCAGG CCCATGGTGG TGAGACTTCC 
ATGAGGAGAG TTTTCAACGT CCTTTACCCC ATGCCTGCGG ACCAGCGGAG ACACGTCAGC 
ATCAACTCTG CCCGCTGGCT ACCTGAGCCA GGGCTCGGTT TGGCCTATGG CACCAACAAA 
GGAGACCTGG TGATCTGCCG ACCAGAGGCC TTAAACTCTG GCATTGAGTA CTACTGGGAC 
CAGCTAAATG AAACGGTCTT CACTGTCCAC TCAAGTAGCA GGAGCAGCGA GCGGCCTGGA 
ACCAGCAGAG CCACATGGCG GACAGACAGA GACATGGGCT TGATGAATGC AATTGGGCTG 
CAACCTCGGA ACCCTACCAC CTCTGTGACA TCTCAGGGCA CCCAGACTCT GGCCCTCCAG 
TTGCAGAATG CTGAAACACA GACTGAGAGG GAGGAAGAGG AACCAGGGAC AGCATCCTCA 
GGTCCTGGTG AAGGCGAGGG CTCGGAATAT GGTGGCAGTG GGGAAGATGC CCTCAGCCGG 
ATCCAGAGGC TAATGGCCGA GGGTGGCATG ACAGCTGTGG TACAGCGAGA ACAGAGTACC 
ACCATGGCCT CCATGGGTGG CTTCGGCAAC AACATCATCG TTAGCCACCG CATTCACCGC 
AGCTCCCAGA CAGGCACAGA GTCAGGGGCT GCTCGTACCT CCTCACCCCA GCCCTCCACC 
TCTCGAGGAC TACTCTCAGA ACCTGGGCAA CTGGCAGAGC GAGGACTAAG CCCCCGGACA 
GCCTCCTGGG ACCAGCCTAG TACCTCTGGA AGGGAACTAC CCCAGCCAGC CCTGTCCTCT 
TCCTCCCCTG TCCCCATTCC AGTCCCCCTT GCCAGCAATG AGGGACCAAC CATGCACTGC 
AATGTGACCA ATAACAGTCA CCTTCCTGAG GGTGATAGTA GCAATGTGGG GGAAGCTGCA 
GGCCCTAGTG GGGAGCCACG GAACCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ambra1 ( XM_008762034.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760256.1 Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X5, mRNA.

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CloneID	ORa43498
Clone ID Related Accession (Same CDS sequence)	XM_008762034.2
Accession Version	XM_008762034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	activating molecule in BECN1-regulated autophagy protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAAGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGTCGCT CTTTGGGGGG CCCACTGTCC AGCCACCCAT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCACGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA ACATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTTTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA TCAGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTT TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCCG GTGAGGCTAG CTCTTCCTGG CATGTCTCCA CTACTTTTGA GGGCATGCCA 
CCGAGTGGCA ACCAACTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAACTCCAGC 
AGGCTGGAGT TGAGCAGTTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CCGGCCATTG GGAACGAATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAGGAAAGCT CTGAGGAAGA CTCCCTGAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760256.1
CDS	204..3746
Translation	MKVVPEKNAVRILWGRERGTRAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPTLDFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHRELAPGLTGSEWTRTVLTLNSRSEVESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPSSGLATESDGGNGSSQNNSGNIRHELQCDLRRFFLEYDRLQELDQSLSGETPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWHVSTTFEGMPPSGNQLPPLERTEGQMPNSSRLELSSSASSQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGIEYYWDQLNETVFTVHSSSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPTTSVTSQGTQTLALQLQNAETQTEREEEEPGTASSGPGEGEGSEYGGSGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTESGAARTSSPQPSTSRGLLSEPGQLAERGLSPRTASWDQPSTSGRELPQPALSSSSPVPIPVPLASNEGPTMHCNVTNNSHLPEGDSSNVGEAAGPSGEPRNR

Target ORF information:

RefSeq Version	XM_008762034.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus autophagy and beclin 1 regulator 1 (Ambra1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762034.2

ORF Insert Sequence:

ATGAAAGTTG TCCCAGAGAA GAATGCTGTA CGAATCCTCT GGGGCCGAGA GCGGGGCACT 
CGTGCCATGG GTGCCCAGCG GCTTCTGCAG GAGCTGGTGG AGGATAAGAC TCGATGGATG 
AAATGGGAGG GCAAGAGAGT AGAACTTCCT GATAGTCCCA CGCTCGACTT CTTACTGGCC 
TTCAGCCCAG ACAGGACTCT CTTGGCGTCC ACACATGTGA ACCATAACAT CTATATTACA 
GAGGTTAAGA CTGGCAAGTG TGTTCACTCT CTGATTGGAC ACCGCCGTAC TCCATGGTGT 
GTCACTTTTC ATCCCACTAT CTCAGGCCTT ATTGCTTCTG GCTGCCTAGA TGGGGAAGTT 
AGGATTTGGG ACTTACATGG TGGCAGTGAA AGCTGGTTCA CAGATAGCAA CAATGCCATA 
GCTTCTTTGG CTTTCCACCC TACGGCTCAG CTCCTGCTGA TCGCCACTGC AAATGAGATC 
CATTTCTGGG ATTGGAGTCG TCGGGAACCC TTTGCTGTGG TGAAAACAGC TAGTGAGATG 
GAGCGTGTTC GTCTGGTGAG ATTTGATCCA CTTGGACACT ACTTACTTAC AGCTATTGTT 
AACCCCTCTA ATCAGCAGGG TGATGATGAA CCAGAAATCC CTATAGATGG AACAGAATTA 
TCTCATTACC GTCAGCGTGC CCTCCTGCAA TCACAGCCAG TTCGCCGGAC ACCCCTCCTC 
CACAATTTCC TGCATATGCT GTCCTCCCGC TCCTCTGGCA TCCAGACCGA GCCCTTCCAT 
CCCCCGGAGC AGGCTTCATC AACGCAGCAG GACCAAGGCC TCCTGAACCG GCCGTCTGCC 
TTCAGTACAG TCCAGAGCAG CACTGCGGGC AACACGCTCC GCAACCTCAG TCTGGGTCCT 
ACCCGTCGCT CTTTGGGGGG CCCACTGTCC AGCCACCCAT CTAGGTATCA CAGAGAACTA 
GCCCCTGGGC TGACAGGTTC TGAGTGGACC CGGACAGTAC TCACTCTGAA CTCACGTTCT 
GAGGTAGAAT CTATGCCCCC ACCGAGGACC AGTGCCTCCT CAGTGAGTTT GCTTTCTGTA 
CTGAGACAGC AGGAAGGTGG CTCTCAGGCA TCTGTGTATA CTTCAGCCAC AGAAGGGAGG 
GGTTTTCCAT CATCAGGGTT GGCAACTGAG TCAGATGGAG GGAATGGTTC CAGCCAAAAC 
AACTCAGGCA ACATTCGCCA TGAGCTTCAG TGTGACCTGA GACGCTTTTT TTTGGAGTAT 
GACAGGCTGC AGGAGCTGGA TCAGAGCCTG AGTGGGGAAA CTCCCCAGAC CCAACAGGCC 
CAGGAAATGC TCAACAATAA TATTGAATCT GAGAGACCAG GTCCTTCCCA TCAGCCTACC 
CCACACAGCA GTGAGAACAA CTCCAACCTT TCTCGGGGCC ACCTTAATCG ATGCCGTGCT 
TGCCATAATC TTCTAACCTT CAACAATGAT ACCCTTCGTT GGGAAAGAAC CACACCTAAC 
TACTCATCCG GTGAGGCTAG CTCTTCCTGG CATGTCTCCA CTACTTTTGA GGGCATGCCA 
CCGAGTGGCA ACCAACTACC ACCTCTTGAG CGGACTGAGG GCCAAATGCC TAACTCCAGC 
AGGCTGGAGT TGAGCAGTTC TGCTAGTTCA CAAGAGGAGA GGACTGTGGG GGTGGCCTTT 
AACCAGGAGA CCGGCCATTG GGAACGAATT TATACCCAGT CCAGCAGATC TGGAACTGTA 
TCACAGGAGG CCTTACATCA AGATATGCCT GAGGAAAGCT CTGAGGAAGA CTCCCTGAGG 
AGATTATCTC CTGCTGCATA CTACGCCCAG AGGATGATCC AGTATCTCTC AAGGAGAGAC 
AGTATTCGCC AGCGCTCCAT GCGCTACCAA CAGAATCGGC TTCGTTCTTC CACCTCCTCT 
TCTTCCTCAG ACAACCAGGG TCCATCAGTA GAGGGAACCG ACTTGGAATT TGAGGACTTT 
GAGGACAATG GTGACAGATC TAGGCACCGA GCTCCCCGAA ATGCCCGAAT GTCTGCACCA 
TCACTTGGAC GCTTTGTCCC AAGGCGTTTT TTGCTGCCTG AGTACTTGCC TTATGCTGGG 
ATTTTTCATG AGCGTGGACA GCCTGGCTTG GCTACTCATT CTTCTGTTAA CAGGGTCCTG 
GCAGGGGCAG TAATTGGAGA TGGACAGTCT GCTGTGGCCA GTAACATTGC CAATACTACC 
TACAGGCTCC AGTGGTGGGA CTTCACCAAG TTTGACCTTC CAGAAATCAG TAATGCCTCC 
GTGAATGTCC TGGTACAGAA CTGCAAGATC TACAATGATG CCAGCTGTGA CATTTCTGCA 
GATGGCCAGC TCCTGGCAGC TTTCATCCCG AGTAGCCAGA GGGGCTTTCC TGATGAAGGC 
ATCCTGGCAG TGTATTCCCT GGCCCCCCAT AACTTGGGGG AAATGCTCTA CACCAAGCGA 
TTTGGTCCCA ATGCCATTTC TGTAAGCCTG TCCCCAATGG GCCGATACGT TATGGTGGGC 
TTGGCCTCAC GAAGGATCCT GCTGCACCCC TCTACAGAGC ACATGGTAGC CCAGGTCTTC 
AGGCTGCAGC AGGCCCATGG TGGTGAGACT TCCATGAGGA GAGTTTTCAA CGTCCTTTAC 
CCCATGCCTG CGGACCAGCG GAGACACGTC AGCATCAACT CTGCCCGCTG GCTACCTGAG 
CCAGGGCTCG GTTTGGCCTA TGGCACCAAC AAAGGAGACC TGGTGATCTG CCGACCAGAG 
GCCTTAAACT CTGGCATTGA GTACTACTGG GACCAGCTAA ATGAAACGGT CTTCACTGTC 
CACTCAAGTA GCAGGAGCAG CGAGCGGCCT GGAACCAGCA GAGCCACATG GCGGACAGAC 
AGAGACATGG GCTTGATGAA TGCAATTGGG CTGCAACCTC GGAACCCTAC CACCTCTGTG 
ACATCTCAGG GCACCCAGAC TCTGGCCCTC CAGTTGCAGA ATGCTGAAAC ACAGACTGAG 
AGGGAGGAAG AGGAACCAGG GACAGCATCC TCAGGTCCTG GTGAAGGCGA GGGCTCGGAA 
TATGGTGGCA GTGGGGAAGA TGCCCTCAGC CGGATCCAGA GGCTAATGGC CGAGGGTGGC 
ATGACAGCTG TGGTACAGCG AGAACAGAGT ACCACCATGG CCTCCATGGG TGGCTTCGGC 
AACAACATCA TCGTTAGCCA CCGCATTCAC CGCAGCTCCC AGACAGGCAC AGAGTCAGGG 
GCTGCTCGTA CCTCCTCACC CCAGCCCTCC ACCTCTCGAG GACTACTCTC AGAACCTGGG 
CAACTGGCAG AGCGAGGACT AAGCCCCCGG ACAGCCTCCT GGGACCAGCC TAGTACCTCT 
GGAAGGGAAC TACCCCAGCC AGCCCTGTCC TCTTCCTCCC CTGTCCCCAT TCCAGTCCCC 
CTTGCCAGCA ATGAGGGACC AACCATGCAC TGCAATGTGA CCAATAACAG TCACCTTCCT 
GAGGGTGATA GTAGCAATGT GGGGGAAGCT GCAGGCCCTA GTGGGGAGCC ACGGAACCGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

AMBRA1, a novel ?-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.
Brain pathology (Zurich, Switzerland)28(1)28-42(2018 Jan)
Miki Y,Tanji K,Mori F,Tatara Y,Utsumi J,Sasaki H,Kakita A,Takahashi H,Fimia GM,Wakabayashi K

IRGM governs the core autophagy machinery to conduct antimicrobial defense.
Molecular cell58(3)507-21(2015 May)
Chauhan S,Mandell MA,Deretic V

AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1.
Cell death and differentiation22(3)419-32(2015 Mar)
Strappazzon F,Nazio F,Corrado M,Cianfanelli V,Romagnoli A,Fimia GM,Campello S,Nardacci R,Piacentini M,Campanella M,Cecconi F

Ambra1 modulates starvation-induced autophagy through AMPK signaling pathway in cardiomyocytes.
Biochemical and biophysical research communications452(3)308-14(2014 Sep)
Shi C,Wu J,Fu M,Zhang B,Wang J,Yang X,Chi Y

Functional interaction between autophagy and ciliogenesis.
Nature502(7470)194-200(2013 Oct)
Pampliega O,Orhon I,Patel B,Sridhar S,D?az-Carretero A,Beau I,Codogno P,Satir BH,Satir P,Cuervo AM

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Ambra1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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