nedd4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nedd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nedd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa36526 XM_012959604.2
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa07375 XM_002940255.5
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa36527 XM_012959605.3
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa43358 XM_018092420.1
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa43359 XM_018092421.1
Latest version!
Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OXa55085 XM_031899176.1
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa55082 XM_031899173.1
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55083 XM_031899174.1
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa55084 XM_031899175.1
Latest version!
Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OXa36527 XM_012959605.2 Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa07375 XM_002940255.4 Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa07375
    Clone ID Related Accession (Same CDS sequence) XM_002940255.4 , XM_002940255.5
    Accession Version XM_002940255.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940255.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG GGTTTCTTTG AACTGATCCC GCAAGACCTG
    ATAAAAATAT TTGATGAAAA TGAGCTGGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940301.2
    CDS183..2873
    Translation

    Target ORF information:

    RefSeq Version XM_002940255.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940255.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG GGTTTCTTTG AACTGATCCC GCAAGACCTG
    ATAAAAATAT TTGATGAAAA TGAGCTGGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36526
    Clone ID Related Accession (Same CDS sequence) XM_012959604.2
    Accession Version XM_012959604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GTTATTACCT
    ACATCTTCTG GATTACCACC TGGCTGGGAA GAAAAACATG ACGATAAAGG AAGATCATAT
    TACATTGATC ACAATAGCAG AACTACTACT TGGGAAAAGC CAAGTAGCCA AGCACAGGGA
    CCCACGGAAA CTTTCCAGAC TTCATCTGTC CAGAATGTCC CTGTCATTTT TCAACCACAA
    ACAGCTACCA GGGAATCCAC ACAGTTATTG GAAACAGAAC AAAGATTTCT CCCCAAAGGA
    TGGGAAGTGC GCCATGCACC AACTGGAAGG CCATTCTATA TCAATCATGT AACCAAAACA
    ACCACATGGG AAGACCCAAG ATTGAAGATT CCAGTACAGC TAAGGCCAAA GCCCCCCATA
    GATCTATCAG ATCTGGGACC ACTGCCTCCT GGATGGGAAG AAAGAACTCA TAATGATGGA
    AGAGTATTTT ACATTGATCA CAACACAAAG CAAACACAGT GGGAGGATCC ACGCTTACAA
    ACTGCAGCAA TAACTGGACC AGCTGTGCCT TACTCCAGGG ATTATAAGAG AAAGTATGAA
    TACTTCCGGA AGAAATTAAA GAAGCAGATT GATATTCCAA ATAGGTTTGA AATGAAGTTA
    CGTCGTACAG CCATTCTTGA AGATTCCTAT AGAAGGATTA TAGCTGTAAA AAGGCCAGAG
    TTTCTCAAGG CACGTCTTTG GATAGAGTTT GATAATGAGA AAGGCTTGGA CTATGGAGGA
    GTGGCCAGAG AATGGTTCTT CTTGATTTCT AAAGAAATGT TCAATCCTTA TTATGGTCTG
    TTTGAATATT CTGCCACGGA CAACTATACT TTACAAATTA ATCCTAATTC TGGTCTGTGT
    AATGAAGATC ACCTTTCGTA TTTCAAGTTT ATAGGCCGCG TGGCTGGAAT GGCAGTATAC
    CATGGAAAGC TTCTTGATGC ATTTTTTATT CGCCCATTTT ACAAAATGAT GCTCCAAAAA
    CCAATTATAT TGTATGACAT GGAGTCTGTG GACAGTGAAT ATTACAATTC CCTCCAGTGG
    ATACTAGAAA ATGATCCTTC AGGTTTAGAT CTGTGCTTCA CTGTTGATGA GGAACTTTTT
    GGACAGACAC ATCAACATGA GTTGAAGGCT GGTGGTTCAC AAATCTTAGT CACAAACAAG
    AACAAAAAAG AGTACATACA TCTTGTTATT CAGTGGAGGT TTATGAACAG AGTTCAAAAA
    CAGATGGCTG CATTCAAAGA GGGTTTCTTT GAACTGATCC CGCAAGACCT GATAAAAATA
    TTTGATGAAA ATGAGCTGGA GCTGCTGATG TGTGGCTTAG GAGATGTAGA TGTTAACAAC
    TGGCGAGAAC ATACAAAGTA CAAGAATGGA TACTCACAAG GACACCAAGT TATACAGTGG
    TTTTGGAAGG CTGTGCTGAT GATGGATGCA GAAAAGAGGA TTCGATTACT TCAGTTTGTC
    ACAGGCACCT CTCGTGTGCC CATGAATGGA TTTGCTGAGC TGTATGGCTC CAATGGACCA
    CAGTTGTTTA CAGTTGAAAA ATGGGGCACT CCAGAAAAAT TACCTAGAGC GCACACATGC
    TTTAATCGTT TGGACTTGCC ACCTTATGAA TCATTTGAGG ACCTGTGGGA TAAGCTCCAT
    ATTGCTATTG AAAATGCTCA AGGTTTTGAT GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815058.1
    CDS184..2865
    Translation

    Target ORF information:

    RefSeq Version XM_012959604.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GTTATTACCT
    ACATCTTCTG GATTACCACC TGGCTGGGAA GAAAAACATG ACGATAAAGG AAGATCATAT
    TACATTGATC ACAATAGCAG AACTACTACT TGGGAAAAGC CAAGTAGCCA AGCACAGGGA
    CCCACGGAAA CTTTCCAGAC TTCATCTGTC CAGAATGTCC CTGTCATTTT TCAACCACAA
    ACAGCTACCA GGGAATCCAC ACAGTTATTG GAAACAGAAC AAAGATTTCT CCCCAAAGGA
    TGGGAAGTGC GCCATGCACC AACTGGAAGG CCATTCTATA TCAATCATGT AACCAAAACA
    ACCACATGGG AAGACCCAAG ATTGAAGATT CCAGTACAGC TAAGGCCAAA GCCCCCCATA
    GATCTATCAG ATCTGGGACC ACTGCCTCCT GGATGGGAAG AAAGAACTCA TAATGATGGA
    AGAGTATTTT ACATTGATCA CAACACAAAG CAAACACAGT GGGAGGATCC ACGCTTACAA
    ACTGCAGCAA TAACTGGACC AGCTGTGCCT TACTCCAGGG ATTATAAGAG AAAGTATGAA
    TACTTCCGGA AGAAATTAAA GAAGCAGATT GATATTCCAA ATAGGTTTGA AATGAAGTTA
    CGTCGTACAG CCATTCTTGA AGATTCCTAT AGAAGGATTA TAGCTGTAAA AAGGCCAGAG
    TTTCTCAAGG CACGTCTTTG GATAGAGTTT GATAATGAGA AAGGCTTGGA CTATGGAGGA
    GTGGCCAGAG AATGGTTCTT CTTGATTTCT AAAGAAATGT TCAATCCTTA TTATGGTCTG
    TTTGAATATT CTGCCACGGA CAACTATACT TTACAAATTA ATCCTAATTC TGGTCTGTGT
    AATGAAGATC ACCTTTCGTA TTTCAAGTTT ATAGGCCGCG TGGCTGGAAT GGCAGTATAC
    CATGGAAAGC TTCTTGATGC ATTTTTTATT CGCCCATTTT ACAAAATGAT GCTCCAAAAA
    CCAATTATAT TGTATGACAT GGAGTCTGTG GACAGTGAAT ATTACAATTC CCTCCAGTGG
    ATACTAGAAA ATGATCCTTC AGGTTTAGAT CTGTGCTTCA CTGTTGATGA GGAACTTTTT
    GGACAGACAC ATCAACATGA GTTGAAGGCT GGTGGTTCAC AAATCTTAGT CACAAACAAG
    AACAAAAAAG AGTACATACA TCTTGTTATT CAGTGGAGGT TTATGAACAG AGTTCAAAAA
    CAGATGGCTG CATTCAAAGA GGGTTTCTTT GAACTGATCC CGCAAGACCT GATAAAAATA
    TTTGATGAAA ATGAGCTGGA GCTGCTGATG TGTGGCTTAG GAGATGTAGA TGTTAACAAC
    TGGCGAGAAC ATACAAAGTA CAAGAATGGA TACTCACAAG GACACCAAGT TATACAGTGG
    TTTTGGAAGG CTGTGCTGAT GATGGATGCA GAAAAGAGGA TTCGATTACT TCAGTTTGTC
    ACAGGCACCT CTCGTGTGCC CATGAATGGA TTTGCTGAGC TGTATGGCTC CAATGGACCA
    CAGTTGTTTA CAGTTGAAAA ATGGGGCACT CCAGAAAAAT TACCTAGAGC GCACACATGC
    TTTAATCGTT TGGACTTGCC ACCTTATGAA TCATTTGAGG ACCTGTGGGA TAAGCTCCAT
    ATTGCTATTG AAAATGCTCA AGGTTTTGAT GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36527
    Clone ID Related Accession (Same CDS sequence) XM_012959605.3 , XM_012959605.2
    Accession Version XM_012959605.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGGT ATATCCACAG AAGCACAGAC TTCTCTTGGA AGTATTTGAT
    GAAAATCGCC TGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815059.1
    CDS183..2834
    Translation

    Target ORF information:

    RefSeq Version XM_012959605.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGGT ATATCCACAG AAGCACAGAC TTCTCTTGGA AGTATTTGAT
    GAAAATCGCC TGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07375
    Clone ID Related Accession (Same CDS sequence) XM_002940255.4 , XM_002940255.5
    Accession Version XM_002940255.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940255.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG GGTTTCTTTG AACTGATCCC GCAAGACCTG
    ATAAAAATAT TTGATGAAAA TGAGCTGGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940301.2
    CDS125..2815
    Translation

    Target ORF information:

    RefSeq Version XM_002940255.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940255.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG GGTTTCTTTG AACTGATCCC GCAAGACCTG
    ATAAAAATAT TTGATGAAAA TGAGCTGGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36527
    Clone ID Related Accession (Same CDS sequence) XM_012959605.3 , XM_012959605.2
    Accession Version XM_012959605.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2652bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959605.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGGT ATATCCACAG AAGCACAGAC TTCTCTTGGA AGTATTTGAT
    GAAAATCGCC TGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815059.1
    CDS125..2776
    Translation

    Target ORF information:

    RefSeq Version XM_012959605.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959605.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGGT ATATCCACAG AAGCACAGAC TTCTCTTGGA AGTATTTGAT
    GAAAATCGCC TGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43358
    Clone ID Related Accession (Same CDS sequence) XM_018092420.1
    Accession Version XM_018092420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2631bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947909.1
    CDS185..2815
    Translation

    Target ORF information:

    RefSeq Version XM_018092420.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATGTTCAT
    TCCAACAGAA GAGAGAGCTT GCAGTCATAC ACTTCAGAAG AACTTCCTAC GGTACCAGTT
    TTATTACCTA CATCTTCTGG ATTACCACCT GGCTGGGAAG AAAAACATGA CGATAAAGGA
    AGATCATATT ACATTGATCA CAATAGCAGA ACTACTACTT GGGAAAAGCC AAGTAGCCAA
    GCACAGGGAC CCACGGAAAC TTTCCAGACT TCATCTGTCC AGAATGTCCC TGTCATTTTT
    CAACCACAAA CAGCTACCAG GGAATCCACA CAGTTATTGG AAACAGAACA AAGATTTCTC
    CCCAAAGGAT GGGAAGTGCG CCATGCACCA ACTGGAAGGC CATTCTATAT CAATCATGTA
    ACCAAAACAA CCACATGGGA AGACCCAAGA TTGAAGATTC CAGTACAGCT AAGGCCAAAG
    CCCCCCATAG ATCTATCAGA TCTGGGACCA CTGCCTCCTG GATGGGAAGA AAGAACTCAT
    AATGATGGAA GAGTATTTTA CATTGATCAC AACACAAAGC AAACACAGTG GGAGGATCCA
    CGCTTACAAA CTGCAGCAAT AACTGGACCA GCTGTGCCTT ACTCCAGGGA TTATAAGAGA
    AAGTATGAAT ACTTCCGGAA GAAATTAAAG AAGCAGATTG ATATTCCAAA TAGGTTTGAA
    ATGAAGTTAC GTCGTACAGC CATTCTTGAA GATTCCTATA GAAGGATTAT AGCTGTAAAA
    AGGCCAGAGT TTCTCAAGGC ACGTCTTTGG ATAGAGTTTG ATAATGAGAA AGGCTTGGAC
    TATGGAGGAG TGGCCAGAGA ATGGTTCTTC TTGATTTCTA AAGAAATGTT CAATCCTTAT
    TATGGTCTGT TTGAATATTC TGCCACGGAC AACTATACTT TACAAATTAA TCCTAATTCT
    GGTCTGTGTA ATGAAGATCA CCTTTCGTAT TTCAAGTTTA TAGGCCGCGT GGCTGGAATG
    GCAGTATACC ATGGAAAGCT TCTTGATGCA TTTTTTATTC GCCCATTTTA CAAAATGATG
    CTCCAAAAAC CAATTATATT GTATGACATG GAGTCTGTGG ACAGTGAATA TTACAATTCC
    CTCCAGTGGA TACTAGAAAA TGATCCTTCA GGTTTAGATC TGTGCTTCAC TGTTGATGAG
    GAACTTTTTG GACAGACACA TCAACATGAG TTGAAGGCTG GTGGTTCACA AATCTTAGTC
    ACAAACAAGA ACAAAAAAGA GTACATACAT CTTGTTATTC AGTGGAGGTT TATGAACAGA
    GTTCAAAAAC AGATGGCTGC ATTCAAAGAG CTGCTGATGT GTGGCTTAGG AGATGTAGAT
    GTTAACAACT GGCGAGAACA TACAAAGTAC AAGAATGGAT ACTCACAAGG ACACCAAGTT
    ATACAGTGGT TTTGGAAGGC TGTGCTGATG ATGGATGCAG AAAAGAGGAT TCGATTACTT
    CAGTTTGTCA CAGGCACCTC TCGTGTGCCC ATGAATGGAT TTGCTGAGCT GTATGGCTCC
    AATGGACCAC AGTTGTTTAC AGTTGAAAAA TGGGGCACTC CAGAAAAATT ACCTAGAGCG
    CACACATGCT TTAATCGTTT GGACTTGCCA CCTTATGAAT CATTTGAGGA CCTGTGGGAT
    AAGCTCCATA TTGCTATTGA AAATGCTCAA GGTTTTGATG GGGTTGATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa43359
    Clone ID Related Accession (Same CDS sequence) XM_018092421.1
    Accession Version XM_018092421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2625bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATTTATTA
    CCTACATCTT CTGGATTACC ACCTGGCTGG GAAGAAAAAC ATGACGATAA AGGAAGATCA
    TATTACATTG ATCACAATAG CAGAACTACT ACTTGGGAAA AGCCAAGTAG CCAAGCACAG
    GGACCCACGG AAACTTTCCA GACTTCATCT GTCCAGAATG TCCCTGTCAT TTTTCAACCA
    CAAACAGCTA CCAGGGAATC CACACAGTTA TTGGAAACAG AACAAAGATT TCTCCCCAAA
    GGATGGGAAG TGCGCCATGC ACCAACTGGA AGGCCATTCT ATATCAATCA TGTAACCAAA
    ACAACCACAT GGGAAGACCC AAGATTGAAG ATTCCAGTAC AGCTAAGGCC AAAGCCCCCC
    ATAGATCTAT CAGATCTGGG ACCACTGCCT CCTGGATGGG AAGAAAGAAC TCATAATGAT
    GGAAGAGTAT TTTACATTGA TCACAACACA AAGCAAACAC AGTGGGAGGA TCCACGCTTA
    CAAACTGCAG CAATAACTGG ACCAGCTGTG CCTTACTCCA GGGATTATAA GAGAAAGTAT
    GAATACTTCC GGAAGAAATT AAAGAAGCAG ATTGATATTC CAAATAGGTT TGAAATGAAG
    TTACGTCGTA CAGCCATTCT TGAAGATTCC TATAGAAGGA TTATAGCTGT AAAAAGGCCA
    GAGTTTCTCA AGGCACGTCT TTGGATAGAG TTTGATAATG AGAAAGGCTT GGACTATGGA
    GGAGTGGCCA GAGAATGGTT CTTCTTGATT TCTAAAGAAA TGTTCAATCC TTATTATGGT
    CTGTTTGAAT ATTCTGCCAC GGACAACTAT ACTTTACAAA TTAATCCTAA TTCTGGTCTG
    TGTAATGAAG ATCACCTTTC GTATTTCAAG TTTATAGGCC GCGTGGCTGG AATGGCAGTA
    TACCATGGAA AGCTTCTTGA TGCATTTTTT ATTCGCCCAT TTTACAAAAT GATGCTCCAA
    AAACCAATTA TATTGTATGA CATGGAGTCT GTGGACAGTG AATATTACAA TTCCCTCCAG
    TGGATACTAG AAAATGATCC TTCAGGTTTA GATCTGTGCT TCACTGTTGA TGAGGAACTT
    TTTGGACAGA CACATCAACA TGAGTTGAAG GCTGGTGGTT CACAAATCTT AGTCACAAAC
    AAGAACAAAA AAGAGTACAT ACATCTTGTT ATTCAGTGGA GGTTTATGAA CAGAGTTCAA
    AAACAGATGG CTGCATTCAA AGAGGGTTTC TTTGAACTGA TCCCGCAAGA CCTGATAAAA
    ATATTTGATG AAAATGAGCT GGAGCTGCTG ATGTGTGGCT TAGGAGATGT AGATGTTAAC
    AACTGGCGAG AACATACAAA GTACAAGAAT GGATACTCAC AAGGACACCA AGTTATACAG
    TGGTTTTGGA AGGCTGTGCT GATGATGGAT GCAGAAAAGA GGATTCGATT ACTTCAGTTT
    GTCACAGGCA CCTCTCGTGT GCCCATGAAT GGATTTGCTG AGCTGTATGG CTCCAATGGA
    CCACAGTTGT TTACAGTTGA AAAATGGGGC ACTCCAGAAA AATTACCTAG AGCGCACACA
    TGCTTTAATC GTTTGGACTT GCCACCTTAT GAATCATTTG AGGACCTGTG GGATAAGCTC
    CATATTGCTA TTGAAAATGC TCAAGGTTTT GATGGGGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017947910.1
    CDS184..2808
    Translation

    Target ORF information:

    RefSeq Version XM_018092421.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (nedd4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018092421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGATGGCGT CATCCATGGA GATTCTTGGT GTCCAAGCAG AAGAGGAAAG TACACGCATT 
    TTACGTGTGA AGGTTATAGC TGGCATTGGA CTGGCAAAGA AAGATATTTT AGGAGCAAGT
    GATCCTTATG TGAAACTGAC TGTGTATGAC CCAGCAAATG GGATCCTGTC AAGTGCTCAA
    ACGAAAACTG TTAGAAAGAC TTTGAATCCT AAATGGAATG AGGAAATATT ATTCAGAGTA
    TATCCACAGA AGCACAGACT TCTCTTGGAA GTATTTGATG AAAATCGCCT GACCAGAGAT
    GATTTTCTTG GGCAAGTGGA TGTTCCTCTT CATCAGTTAC CAACAGAAAA CCCACGGTTG
    GAGCGACCAT ATACATTCAA AGACTTTATT CTTCATCCAA GAAGCCACAA ATCACGAGTC
    AAAGGAAATC TACGGTTAAA AATGACATAT TTACCTAAAA ACAATGGATC AGAAGATGAA
    AATGCAGAGC AGGCTGAAGA AATGGAGCCT GGCTGGGTTA TACTGGACCA ACCAGATACA
    TCTTGCCAAC CTCAACAAGA ACAAGAACTT TCCCCACTAC CTCCAGGATG GGAAGAGAGA
    CAGGATATCC TTGGAAGAAC TTACTATGTC AGTCATCAGT ACAGAAGAAC ACAGTGGCAA
    AGGCCTACTA TTCAAGATGA TACTGCAGCC AATGAAAGCA GGAATGTTCA GTTAGAGGAA
    CAGCATCATC ATGCATTCAC AACAAGGAGG CAAATATCTG AAGAAACAGA AAGCAGAGAT
    TCTCCAGAGA GCTGGGAAAT TATAACAGAA GATGAAACAA CAATCTATAG CAACCAGAAT
    GCCCAGCAGG GATCTGCACC AACCAGTGAT GCCCAGGCAC AAATCACAGA AGAGCTTAGT
    ATAAGGCTTA ACATTTCTGG GAATATGCCA AACAGCCGAC AGAGCTCTTC CAATTTATTA
    CCTACATCTT CTGGATTACC ACCTGGCTGG GAAGAAAAAC ATGACGATAA AGGAAGATCA
    TATTACATTG ATCACAATAG CAGAACTACT ACTTGGGAAA AGCCAAGTAG CCAAGCACAG
    GGACCCACGG AAACTTTCCA GACTTCATCT GTCCAGAATG TCCCTGTCAT TTTTCAACCA
    CAAACAGCTA CCAGGGAATC CACACAGTTA TTGGAAACAG AACAAAGATT TCTCCCCAAA
    GGATGGGAAG TGCGCCATGC ACCAACTGGA AGGCCATTCT ATATCAATCA TGTAACCAAA
    ACAACCACAT GGGAAGACCC AAGATTGAAG ATTCCAGTAC AGCTAAGGCC AAAGCCCCCC
    ATAGATCTAT CAGATCTGGG ACCACTGCCT CCTGGATGGG AAGAAAGAAC TCATAATGAT
    GGAAGAGTAT TTTACATTGA TCACAACACA AAGCAAACAC AGTGGGAGGA TCCACGCTTA
    CAAACTGCAG CAATAACTGG ACCAGCTGTG CCTTACTCCA GGGATTATAA GAGAAAGTAT
    GAATACTTCC GGAAGAAATT AAAGAAGCAG ATTGATATTC CAAATAGGTT TGAAATGAAG
    TTACGTCGTA CAGCCATTCT TGAAGATTCC TATAGAAGGA TTATAGCTGT AAAAAGGCCA
    GAGTTTCTCA AGGCACGTCT TTGGATAGAG TTTGATAATG AGAAAGGCTT GGACTATGGA
    GGAGTGGCCA GAGAATGGTT CTTCTTGATT TCTAAAGAAA TGTTCAATCC TTATTATGGT
    CTGTTTGAAT ATTCTGCCAC GGACAACTAT ACTTTACAAA TTAATCCTAA TTCTGGTCTG
    TGTAATGAAG ATCACCTTTC GTATTTCAAG TTTATAGGCC GCGTGGCTGG AATGGCAGTA
    TACCATGGAA AGCTTCTTGA TGCATTTTTT ATTCGCCCAT TTTACAAAAT GATGCTCCAA
    AAACCAATTA TATTGTATGA CATGGAGTCT GTGGACAGTG AATATTACAA TTCCCTCCAG
    TGGATACTAG AAAATGATCC TTCAGGTTTA GATCTGTGCT TCACTGTTGA TGAGGAACTT
    TTTGGACAGA CACATCAACA TGAGTTGAAG GCTGGTGGTT CACAAATCTT AGTCACAAAC
    AAGAACAAAA AAGAGTACAT ACATCTTGTT ATTCAGTGGA GGTTTATGAA CAGAGTTCAA
    AAACAGATGG CTGCATTCAA AGAGGGTTTC TTTGAACTGA TCCCGCAAGA CCTGATAAAA
    ATATTTGATG AAAATGAGCT GGAGCTGCTG ATGTGTGGCT TAGGAGATGT AGATGTTAAC
    AACTGGCGAG AACATACAAA GTACAAGAAT GGATACTCAC AAGGACACCA AGTTATACAG
    TGGTTTTGGA AGGCTGTGCT GATGATGGAT GCAGAAAAGA GGATTCGATT ACTTCAGTTT
    GTCACAGGCA CCTCTCGTGT GCCCATGAAT GGATTTGCTG AGCTGTATGG CTCCAATGGA
    CCACAGTTGT TTACAGTTGA AAAATGGGGC ACTCCAGAAA AATTACCTAG AGCGCACACA
    TGCTTTAATC GTTTGGACTT GCCACCTTAT GAATCATTTG AGGACCTGTG GGATAAGCTC
    CATATTGCTA TTGAAAATGC TCAAGGTTTT GATGGGGTTG ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55085
    Clone ID Related Accession (Same CDS sequence) XM_031899176.1
    Accession Version XM_031899176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATATACA CCTGATTCCA AAGATTGAAA GACACCATAA TAACTCCAAC
    AAGCTGTTTT CCAAGAATCA TTTTGCTGAG AATGTAATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755036.1
    CDS900..3101
    Translation

    Target ORF information:

    RefSeq Version XM_031899176.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATATACA CCTGATTCCA AAGATTGAAA GACACCATAA TAACTCCAAC
    AAGCTGTTTT CCAAGAATCA TTTTGCTGAG AATGTAATGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55082
    Clone ID Related Accession (Same CDS sequence) XM_031899173.1
    Accession Version XM_031899173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755033.1
    CDS664..4515
    Translation

    Target ORF information:

    RefSeq Version XM_031899173.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGGTACCAGT TTTATTACCT ACATCTTCTG GATTACCACC TGGCTGGGAA
    GAAAAACATG ACGATAAAGG AAGATCATAT TACATTGATC ACAATAGCAG AACTACTACT
    TGGGAAAAGC CAAGTAGCCA AGCACAGGGA CCCACGGAAA CTTTCCAGAC TTCATCTGTC
    CAGAATGTCC CTGTCATTTT TCAACCACAA ACAGCTACCA GGGAATCCAC ACAGTTATTG
    GAAACAGAAC AAAGATTTCT CCCCAAAGGA TGGGAAGTGC GCCATGCACC AACTGGAAGG
    CCATTCTATA TCAATCATGT AACCAAAACA ACCACATGGG AAGACCCAAG ATTGAAGATT
    CCAGTACAGC TAAGGCCAAA GCCCCCCATA GATCTATCAG ATCTGGGACC ACTGCCTCCT
    GGATGGGAAG AAAGAACTCA TAATGATGGA AGAGTATTTT ACATTGATCA CAACACAAAG
    CAAACACAGT GGGAGGATCC ACGCTTACAA ACTGCAGCAA TAACTGGACC AGCTGTGCCT
    TACTCCAGGG ATTATAAGAG AAAGTATGAA TACTTCCGGA AGAAATTAAA GAAGCAGATT
    GATATTCCAA ATAGGTTTGA AATGAAGTTA CGTCGTACAG CCATTCTTGA AGATTCCTAT
    AGAAGGATTA TAGCTGTAAA AAGGCCAGAG TTTCTCAAGG CACGTCTTTG GATAGAGTTT
    GATAATGAGA AAGGCTTGGA CTATGGAGGA GTGGCCAGAG AATGGTTCTT CTTGATTTCT
    AAAGAAATGT TCAATCCTTA TTATGGTCTG TTTGAATATT CTGCCACGGA CAACTATACT
    TTACAAATTA ATCCTAATTC TGGTCTGTGT AATGAAGATC ACCTTTCGTA TTTCAAGTTT
    ATAGGCCGCG TGGCTGGAAT GGCAGTATAC CATGGAAAGC TTCTTGATGC ATTTTTTATT
    CGCCCATTTT ACAAAATGAT GCTCCAAAAA CCAATTATAT TGTATGACAT GGAGTCTGTG
    GACAGTGAAT ATTACAATTC CCTCCAGTGG ATACTAGAAA ATGATCCTTC AGGTTTAGAT
    CTGTGCTTCA CTGTTGATGA GGAACTTTTT GGACAGACAC ATCAACATGA GTTGAAGGCT
    GGTGGTTCAC AAATCTTAGT CACAAACAAG AACAAAAAAG AGTACATACA TCTTGTTATT
    CAGTGGAGGT TTATGAACAG AGTTCAAAAA CAGATGGCTG CATTCAAAGA GGGTTTCTTT
    GAACTGATCC CGCAAGACCT GATAAAAATA TTTGATGAAA ATGAGCTGGA GCTGCTGATG
    TGTGGCTTAG GAGATGTAGA TGTTAACAAC TGGCGAGAAC ATACAAAGTA CAAGAATGGA
    TACTCACAAG GACACCAAGT TATACAGTGG TTTTGGAAGG CTGTGCTGAT GATGGATGCA
    GAAAAGAGGA TTCGATTACT TCAGTTTGTC ACAGGCACCT CTCGTGTGCC CATGAATGGA
    TTTGCTGAGC TGTATGGCTC CAATGGACCA CAGTTGTTTA CAGTTGAAAA ATGGGGCACT
    CCAGAAAAAT TACCTAGAGC GCACACATGC TTTAATCGTT TGGACTTGCC ACCTTATGAA
    TCATTTGAGG ACCTGTGGGA TAAGCTCCAT ATTGCTATTG AAAATGCTCA AGGTTTTGAT
    GGGGTTGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55083
    Clone ID Related Accession (Same CDS sequence) XM_031899174.1
    Accession Version XM_031899174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGTTATTACC TACATCTTCT GGATTACCAC CTGGCTGGGA AGAAAAACAT
    GACGATAAAG GAAGATCATA TTACATTGAT CACAATAGCA GAACTACTAC TTGGGAAAAG
    CCAAGTAGCC AAGCACAGGG ACCCACGGAA ACTTTCCAGA CTTCATCTGT CCAGAATGTC
    CCTGTCATTT TTCAACCACA AACAGCTACC AGGGAATCCA CACAGTTATT GGAAACAGAA
    CAAAGATTTC TCCCCAAAGG ATGGGAAGTG CGCCATGCAC CAACTGGAAG GCCATTCTAT
    ATCAATCATG TAACCAAAAC AACCACATGG GAAGACCCAA GATTGAAGAT TCCAGTACAG
    CTAAGGCCAA AGCCCCCCAT AGATCTATCA GATCTGGGAC CACTGCCTCC TGGATGGGAA
    GAAAGAACTC ATAATGATGG AAGAGTATTT TACATTGATC ACAACACAAA GCAAACACAG
    TGGGAGGATC CACGCTTACA AACTGCAGCA ATAACTGGAC CAGCTGTGCC TTACTCCAGG
    GATTATAAGA GAAAGTATGA ATACTTCCGG AAGAAATTAA AGAAGCAGAT TGATATTCCA
    AATAGGTTTG AAATGAAGTT ACGTCGTACA GCCATTCTTG AAGATTCCTA TAGAAGGATT
    ATAGCTGTAA AAAGGCCAGA GTTTCTCAAG GCACGTCTTT GGATAGAGTT TGATAATGAG
    AAAGGCTTGG ACTATGGAGG AGTGGCCAGA GAATGGTTCT TCTTGATTTC TAAAGAAATG
    TTCAATCCTT ATTATGGTCT GTTTGAATAT TCTGCCACGG ACAACTATAC TTTACAAATT
    AATCCTAATT CTGGTCTGTG TAATGAAGAT CACCTTTCGT ATTTCAAGTT TATAGGCCGC
    GTGGCTGGAA TGGCAGTATA CCATGGAAAG CTTCTTGATG CATTTTTTAT TCGCCCATTT
    TACAAAATGA TGCTCCAAAA ACCAATTATA TTGTATGACA TGGAGTCTGT GGACAGTGAA
    TATTACAATT CCCTCCAGTG GATACTAGAA AATGATCCTT CAGGTTTAGA TCTGTGCTTC
    ACTGTTGATG AGGAACTTTT TGGACAGACA CATCAACATG AGTTGAAGGC TGGTGGTTCA
    CAAATCTTAG TCACAAACAA GAACAAAAAA GAGTACATAC ATCTTGTTAT TCAGTGGAGG
    TTTATGAACA GAGTTCAAAA ACAGATGGCT GCATTCAAAG AGGGTTTCTT TGAACTGATC
    CCGCAAGACC TGATAAAAAT ATTTGATGAA AATGAGCTGG AGCTGCTGAT GTGTGGCTTA
    GGAGATGTAG ATGTTAACAA CTGGCGAGAA CATACAAAGT ACAAGAATGG ATACTCACAA
    GGACACCAAG TTATACAGTG GTTTTGGAAG GCTGTGCTGA TGATGGATGC AGAAAAGAGG
    ATTCGATTAC TTCAGTTTGT CACAGGCACC TCTCGTGTGC CCATGAATGG ATTTGCTGAG
    CTGTATGGCT CCAATGGACC ACAGTTGTTT ACAGTTGAAA AATGGGGCAC TCCAGAAAAA
    TTACCTAGAG CGCACACATG CTTTAATCGT TTGGACTTGC CACCTTATGA ATCATTTGAG
    GACCTGTGGG ATAAGCTCCA TATTGCTATT GAAAATGCTC AAGGTTTTGA TGGGGTTGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755034.1
    CDS665..4507
    Translation

    Target ORF information:

    RefSeq Version XM_031899174.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATGTTCA TTCCAACAGA AGAGAGAGCT TGCAGTCATA CACTTCAGAA
    GAACTTCCTA CGTTATTACC TACATCTTCT GGATTACCAC CTGGCTGGGA AGAAAAACAT
    GACGATAAAG GAAGATCATA TTACATTGAT CACAATAGCA GAACTACTAC TTGGGAAAAG
    CCAAGTAGCC AAGCACAGGG ACCCACGGAA ACTTTCCAGA CTTCATCTGT CCAGAATGTC
    CCTGTCATTT TTCAACCACA AACAGCTACC AGGGAATCCA CACAGTTATT GGAAACAGAA
    CAAAGATTTC TCCCCAAAGG ATGGGAAGTG CGCCATGCAC CAACTGGAAG GCCATTCTAT
    ATCAATCATG TAACCAAAAC AACCACATGG GAAGACCCAA GATTGAAGAT TCCAGTACAG
    CTAAGGCCAA AGCCCCCCAT AGATCTATCA GATCTGGGAC CACTGCCTCC TGGATGGGAA
    GAAAGAACTC ATAATGATGG AAGAGTATTT TACATTGATC ACAACACAAA GCAAACACAG
    TGGGAGGATC CACGCTTACA AACTGCAGCA ATAACTGGAC CAGCTGTGCC TTACTCCAGG
    GATTATAAGA GAAAGTATGA ATACTTCCGG AAGAAATTAA AGAAGCAGAT TGATATTCCA
    AATAGGTTTG AAATGAAGTT ACGTCGTACA GCCATTCTTG AAGATTCCTA TAGAAGGATT
    ATAGCTGTAA AAAGGCCAGA GTTTCTCAAG GCACGTCTTT GGATAGAGTT TGATAATGAG
    AAAGGCTTGG ACTATGGAGG AGTGGCCAGA GAATGGTTCT TCTTGATTTC TAAAGAAATG
    TTCAATCCTT ATTATGGTCT GTTTGAATAT TCTGCCACGG ACAACTATAC TTTACAAATT
    AATCCTAATT CTGGTCTGTG TAATGAAGAT CACCTTTCGT ATTTCAAGTT TATAGGCCGC
    GTGGCTGGAA TGGCAGTATA CCATGGAAAG CTTCTTGATG CATTTTTTAT TCGCCCATTT
    TACAAAATGA TGCTCCAAAA ACCAATTATA TTGTATGACA TGGAGTCTGT GGACAGTGAA
    TATTACAATT CCCTCCAGTG GATACTAGAA AATGATCCTT CAGGTTTAGA TCTGTGCTTC
    ACTGTTGATG AGGAACTTTT TGGACAGACA CATCAACATG AGTTGAAGGC TGGTGGTTCA
    CAAATCTTAG TCACAAACAA GAACAAAAAA GAGTACATAC ATCTTGTTAT TCAGTGGAGG
    TTTATGAACA GAGTTCAAAA ACAGATGGCT GCATTCAAAG AGGGTTTCTT TGAACTGATC
    CCGCAAGACC TGATAAAAAT ATTTGATGAA AATGAGCTGG AGCTGCTGAT GTGTGGCTTA
    GGAGATGTAG ATGTTAACAA CTGGCGAGAA CATACAAAGT ACAAGAATGG ATACTCACAA
    GGACACCAAG TTATACAGTG GTTTTGGAAG GCTGTGCTGA TGATGGATGC AGAAAAGAGG
    ATTCGATTAC TTCAGTTTGT CACAGGCACC TCTCGTGTGC CCATGAATGG ATTTGCTGAG
    CTGTATGGCT CCAATGGACC ACAGTTGTTT ACAGTTGAAA AATGGGGCAC TCCAGAAAAA
    TTACCTAGAG CGCACACATG CTTTAATCGT TTGGACTTGC CACCTTATGA ATCATTTGAG
    GACCTGTGGG ATAAGCTCCA TATTGCTATT GAAAATGCTC AAGGTTTTGA TGGGGTTGAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55084
    Clone ID Related Accession (Same CDS sequence) XM_031899175.1
    Accession Version XM_031899175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATTTATT ACCTACATCT TCTGGATTAC CACCTGGCTG GGAAGAAAAA
    CATGACGATA AAGGAAGATC ATATTACATT GATCACAATA GCAGAACTAC TACTTGGGAA
    AAGCCAAGTA GCCAAGCACA GGGACCCACG GAAACTTTCC AGACTTCATC TGTCCAGAAT
    GTCCCTGTCA TTTTTCAACC ACAAACAGCT ACCAGGGAAT CCACACAGTT ATTGGAAACA
    GAACAAAGAT TTCTCCCCAA AGGATGGGAA GTGCGCCATG CACCAACTGG AAGGCCATTC
    TATATCAATC ATGTAACCAA AACAACCACA TGGGAAGACC CAAGATTGAA GATTCCAGTA
    CAGCTAAGGC CAAAGCCCCC CATAGATCTA TCAGATCTGG GACCACTGCC TCCTGGATGG
    GAAGAAAGAA CTCATAATGA TGGAAGAGTA TTTTACATTG ATCACAACAC AAAGCAAACA
    CAGTGGGAGG ATCCACGCTT ACAAACTGCA GCAATAACTG GACCAGCTGT GCCTTACTCC
    AGGGATTATA AGAGAAAGTA TGAATACTTC CGGAAGAAAT TAAAGAAGCA GATTGATATT
    CCAAATAGGT TTGAAATGAA GTTACGTCGT ACAGCCATTC TTGAAGATTC CTATAGAAGG
    ATTATAGCTG TAAAAAGGCC AGAGTTTCTC AAGGCACGTC TTTGGATAGA GTTTGATAAT
    GAGAAAGGCT TGGACTATGG AGGAGTGGCC AGAGAATGGT TCTTCTTGAT TTCTAAAGAA
    ATGTTCAATC CTTATTATGG TCTGTTTGAA TATTCTGCCA CGGACAACTA TACTTTACAA
    ATTAATCCTA ATTCTGGTCT GTGTAATGAA GATCACCTTT CGTATTTCAA GTTTATAGGC
    CGCGTGGCTG GAATGGCAGT ATACCATGGA AAGCTTCTTG ATGCATTTTT TATTCGCCCA
    TTTTACAAAA TGATGCTCCA AAAACCAATT ATATTGTATG ACATGGAGTC TGTGGACAGT
    GAATATTACA ATTCCCTCCA GTGGATACTA GAAAATGATC CTTCAGGTTT AGATCTGTGC
    TTCACTGTTG ATGAGGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA GGCTGGTGGT
    TCACAAATCT TAGTCACAAA CAAGAACAAA AAAGAGTACA TACATCTTGT TATTCAGTGG
    AGGTTTATGA ACAGAGTTCA AAAACAGATG GCTGCATTCA AAGAGGGTTT CTTTGAACTG
    ATCCCGCAAG ACCTGATAAA AATATTTGAT GAAAATGAGC TGGAGCTGCT GATGTGTGGC
    TTAGGAGATG TAGATGTTAA CAACTGGCGA GAACATACAA AGTACAAGAA TGGATACTCA
    CAAGGACACC AAGTTATACA GTGGTTTTGG AAGGCTGTGC TGATGATGGA TGCAGAAAAG
    AGGATTCGAT TACTTCAGTT TGTCACAGGC ACCTCTCGTG TGCCCATGAA TGGATTTGCT
    GAGCTGTATG GCTCCAATGG ACCACAGTTG TTTACAGTTG AAAAATGGGG CACTCCAGAA
    AAATTACCTA GAGCGCACAC ATGCTTTAAT CGTTTGGACT TGCCACCTTA TGAATCATTT
    GAGGACCTGT GGGATAAGCT CCATATTGCT ATTGAAAATG CTCAAGGTTT TGATGGGGTT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031755035.1
    CDS665..4450
    Translation

    Target ORF information:

    RefSeq Version XM_031899175.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NEDD4 E3 ubiquitin protein ligase (nedd4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031899175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTCGGC GGTTAAGACT TCACTTTGCA ACTAGGAGAA GCAACACTGA TCCCTTACCA 
    GAAAGCTCTG GAGATGGCTT GGATAGTAAT ATTCATATGT GCTTTAAAAG ACTCTCTCCA
    ACAACTGCAC CCGGTGTGAT TCAAATGAGG CTGTCTCCGA GGCAAAGTGT GCAGAAGTCC
    TCTTCCCCAG AAACTATTCC TATACATCAA GGTCATTCTG TTTCTCCACC ATGCAAAAAA
    AGCACTTCAG TGCAGATTTC TTTACAGCCA AGCAGGAATA CTAGATGTCA TCAATCTGGC
    AACACTGCGC CTGCTATGGA AGATATTTGC GTCGTCAGTT CTTACAAGGA TGGAATTAAT
    TCTACTATGA GCAGGAATTA TAACTGTCAA CCCACCATAA ATGACACCTG CATCAGTAGT
    AGTACCTCAG CTGAAAATGG CAGTTTATTA AGCATTTCTG CAAGTGACAA TGGATCATAT
    AGTAGCAACA GCAGTGACTA TGGCTCCTGT GCAAGTACCG CAAGTGATGC TGGCTCGTGT
    GCTAACAGTA TAGTAAGTGA CTGCAGTAGC TGTACTTATT CTTTAAGTGA CAGCAATTAC
    TTAAATGTAT TACCCAATGG TAATGCCCTG TTCTTTAACC CAGAAGAAAA CTGTGAGTTT
    TTTAAAAGTA AACTGGAAGA ACCTTTGCCT ATAAATAACT GCAAGTTCCA TTTTGACCTG
    GATCACTCAG AAAAAGAGGA GGTCAGAATT CCTGCAAGGA AGAAAACAAG AATTCCATTA
    AGACGCTGCT CTTCATTAGT TATTTTTCCT TCTGACACTC CACCAACATC TCCAACTAGT
    CTAGTCCCTC CATTAAGCAG GGGCTGCTAT CAAACTTCTT ACCAGCTTAT GCTATCCTCT
    AATGATTTTA CACAAAAAGA GGAGCAAGCA GCCTCCAAGG GGTCCCTTTC AACAGCAGTC
    AATGGGGTTC GGCTTTCCAA AAACACCTGC AATGTTGGTG AGGTTAGAGA TGTTAAGCCG
    CTTTACTTGA ATGACAAAGA TGATGGACTG CCAAATATTT GCAATTCTGT TGCAGAACAC
    AGCAAACTGC ATGAGGATAA ACCTCAGCTG AACTCAGATG CACGGCACTG TCGGCTGGTG
    CGCAGCACAT CCGCATATTC TCCCTCCAAT AAAACTGTGG AATGCCAGAT GGGGAGAAGC
    TCAGAGCAGG GATACAAAAA TATTCACAAG TGCCAATTAA CTCATGTTTT TCAAAGAAGT
    GTCTCTGAAG GTCCCCCACA AAAACGGTCC TCTGCCTCTA GCCTTAAATG TAGACATCCA
    AAATCAGGGT CCTCTTATTT GCACATACAG TTTGCCCCTG GAAACGAAAG CAGGATTAGT
    AGCTATAAGA AACAATCTTT AAGAAGCCCC ACAACAGAAC TGCCTGGTAA GCCTTCTGTG
    AAATTACTTA AGACCAGAGA TGATTTTCTT GGGCAAGTGG ATGTTCCTCT TCATCAGTTA
    CCAACAGAAA ACCCACGGTT GGAGCGACCA TATACATTCA AAGACTTTAT TCTTCATCCA
    AGAAGCCACA AATCACGAGT CAAAGGAAAT CTACGGTTAA AAATGACATA TTTACCTAAA
    AACAATGGAT CAGAAGATGA AAATGCAGAG CAGGCTGAAG AAATGGAGCC TGGCTGGGTT
    ATACTGGACC AACCAGATAC ATCTTGCCAA CCTCAACAAG AACAAGAACT TTCCCCACTA
    CCTCCAGGAT GGGAAGAGAG ACAGGATATC CTTGGAAGAA CTTACTATGT CAGTCATCAG
    TACAGAAGAA CACAGTGGCA AAGGCCTACT ATTCAAGATG ATACTGCAGC CAATGAAAGC
    AGGAATGTTC AGTTAGAGGA ACAGCATCAT CATGCATTCA CAACAAGGAG GCAAATATCT
    GAAGAAACAG AAAGCAGAGA TTCTCCAGAG AGCTGGGAAA TTATAACAGA AGATGAAACA
    ACAATCTATA GCAACCAGAA TGCCCAGCAG GGATCTGCAC CAACCAGTGA TGCCCAGGCA
    CAAATCACAG AAGAGCTTAG TATAAGGCTT AACATTTCTG GGAATATGCC AAACAGCCGA
    CAGAGCTCTT CCAATTTATT ACCTACATCT TCTGGATTAC CACCTGGCTG GGAAGAAAAA
    CATGACGATA AAGGAAGATC ATATTACATT GATCACAATA GCAGAACTAC TACTTGGGAA
    AAGCCAAGTA GCCAAGCACA GGGACCCACG GAAACTTTCC AGACTTCATC TGTCCAGAAT
    GTCCCTGTCA TTTTTCAACC ACAAACAGCT ACCAGGGAAT CCACACAGTT ATTGGAAACA
    GAACAAAGAT TTCTCCCCAA AGGATGGGAA GTGCGCCATG CACCAACTGG AAGGCCATTC
    TATATCAATC ATGTAACCAA AACAACCACA TGGGAAGACC CAAGATTGAA GATTCCAGTA
    CAGCTAAGGC CAAAGCCCCC CATAGATCTA TCAGATCTGG GACCACTGCC TCCTGGATGG
    GAAGAAAGAA CTCATAATGA TGGAAGAGTA TTTTACATTG ATCACAACAC AAAGCAAACA
    CAGTGGGAGG ATCCACGCTT ACAAACTGCA GCAATAACTG GACCAGCTGT GCCTTACTCC
    AGGGATTATA AGAGAAAGTA TGAATACTTC CGGAAGAAAT TAAAGAAGCA GATTGATATT
    CCAAATAGGT TTGAAATGAA GTTACGTCGT ACAGCCATTC TTGAAGATTC CTATAGAAGG
    ATTATAGCTG TAAAAAGGCC AGAGTTTCTC AAGGCACGTC TTTGGATAGA GTTTGATAAT
    GAGAAAGGCT TGGACTATGG AGGAGTGGCC AGAGAATGGT TCTTCTTGAT TTCTAAAGAA
    ATGTTCAATC CTTATTATGG TCTGTTTGAA TATTCTGCCA CGGACAACTA TACTTTACAA
    ATTAATCCTA ATTCTGGTCT GTGTAATGAA GATCACCTTT CGTATTTCAA GTTTATAGGC
    CGCGTGGCTG GAATGGCAGT ATACCATGGA AAGCTTCTTG ATGCATTTTT TATTCGCCCA
    TTTTACAAAA TGATGCTCCA AAAACCAATT ATATTGTATG ACATGGAGTC TGTGGACAGT
    GAATATTACA ATTCCCTCCA GTGGATACTA GAAAATGATC CTTCAGGTTT AGATCTGTGC
    TTCACTGTTG ATGAGGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA GGCTGGTGGT
    TCACAAATCT TAGTCACAAA CAAGAACAAA AAAGAGTACA TACATCTTGT TATTCAGTGG
    AGGTTTATGA ACAGAGTTCA AAAACAGATG GCTGCATTCA AAGAGGGTTT CTTTGAACTG
    ATCCCGCAAG ACCTGATAAA AATATTTGAT GAAAATGAGC TGGAGCTGCT GATGTGTGGC
    TTAGGAGATG TAGATGTTAA CAACTGGCGA GAACATACAA AGTACAAGAA TGGATACTCA
    CAAGGACACC AAGTTATACA GTGGTTTTGG AAGGCTGTGC TGATGATGGA TGCAGAAAAG
    AGGATTCGAT TACTTCAGTT TGTCACAGGC ACCTCTCGTG TGCCCATGAA TGGATTTGCT
    GAGCTGTATG GCTCCAATGG ACCACAGTTG TTTACAGTTG AAAAATGGGG CACTCCAGAA
    AAATTACCTA GAGCGCACAC ATGCTTTAAT CGTTTGGACT TGCCACCTTA TGAATCATTT
    GAGGACCTGT GGGATAAGCT CCATATTGCT ATTGAAAATG CTCAAGGTTT TGATGGGGTT
    GATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.