NEDD4 cDNA ORF clone, Gallus gallus(chicken)

The following NEDD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa143447 XM_015291879.2
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa143448 XM_015291880.2
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa143449 XM_015291881.2
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OGa40464 XM_015291878.1
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa40465 XM_015291879.1
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa40466 XM_015291880.1
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OGa40467 XM_015291881.1
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa143446 XM_015291877.2
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa40463 XM_015291877.1
Latest version!
Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa143447
    Clone ID Related Accession (Same CDS sequence) XM_015291879.2
    Accession Version XM_015291879.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAAGGGT CGGCTGCATC CTGGAATTGG TACAAAGGAG GCGATACCAG GATTTGGGAG 
    CAGGAGAACA CACGAATTGT CAGGGTGAAG GTTATAGCTG GAATTGGTCT GGCCAAGAAG
    GATATCCTAG GAGCCAGTGA CCCATATGTG AAAGTGACAG TGTATGATCC AGTGAATGGA
    GTCCTTAGCA GTGTTCAGAC AAAAACTATT AGAAAGTCCT TGAACCCAAA GTGGAATGAA
    GAACTTTTAT TTCGAGTTAA TCCTCAGAAA CACAGACTCC TTTTTGAAGT GTTTGATGAA
    AACCGTTTGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGA TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTTCAAATG GACCACAGTT GTTTACAGTT
    GAACAGTGGG GTACCCCTGA AAAACTGCCG AGAGCTCATA CCTGCTTTAA TCGCTTGGAT
    TTGCCCCCGT ATGATTCATT TGAAGATTTA TGGGATAAAC TTCTTCTAGC GATTGAAAAT
    ACTCAGGGCT TTGATGGGGT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147365.2
    CDS74..2800
    Translation

    Target ORF information:

    RefSeq Version XM_015291879.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291879.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAAGGGT CGGCTGCATC CTGGAATTGG TACAAAGGAG GCGATACCAG GATTTGGGAG 
    CAGGAGAACA CACGAATTGT CAGGGTGAAG GTTATAGCTG GAATTGGTCT GGCCAAGAAG
    GATATCCTAG GAGCCAGTGA CCCATATGTG AAAGTGACAG TGTATGATCC AGTGAATGGA
    GTCCTTAGCA GTGTTCAGAC AAAAACTATT AGAAAGTCCT TGAACCCAAA GTGGAATGAA
    GAACTTTTAT TTCGAGTTAA TCCTCAGAAA CACAGACTCC TTTTTGAAGT GTTTGATGAA
    AACCGTTTGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGA TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTTCAAATG GACCACAGTT GTTTACAGTT
    GAACAGTGGG GTACCCCTGA AAAACTGCCG AGAGCTCATA CCTGCTTTAA TCGCTTGGAT
    TTGCCCCCGT ATGATTCATT TGAAGATTTA TGGGATAAAC TTCTTCTAGC GATTGAAAAT
    ACTCAGGGCT TTGATGGGGT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143448
    Clone ID Related Accession (Same CDS sequence) XM_015291880.2
    Accession Version XM_015291880.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2709bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGCCT CTCCTGTGGA GGTGTTCGGG CTGCCCGGCG AGGAGGAGAA CACACGAATT 
    GTCAGGGTGA AGGTTATAGC TGGAATTGGT CTGGCCAAGA AGGATATCCT AGGAGCCAGT
    GACCCATATG TGAAAGTGAC AGTGTATGAT CCAGTGAATG GAGTCCTTAG CAGTGTTCAG
    ACAAAAACTA TTAGAAAGTC CTTGAACCCA AAGTGGAATG AAGAACTTTT ATTTCGAGTT
    AATCCTCAGA AACACAGACT CCTTTTTGAA GTGTTTGATG AAAACCGTTT GACTCGAGAT
    GATTTTCTTG GGCAAGTGGA TATTCCCCTT TATCAGTTAC CGACAGAACA TCCAAGTATG
    GAGAGACCAT ATACCTTTAG GGATTTTGTT CTTCATCCAA GAAGCCATAA ATCAAGAGTT
    AAAGGTCACC TTAGATTAAA GATGACTTAC TTACCTAAAA GCAATGGATC TGAAGAAGAA
    ACCACAGAAC AGGCCGAGGA ATTGGAGCCT GGGTGGATTT TATTGGACCA ACCGGATGCT
    TCTAACCAGC CACAACAGCA GCAGCAGGAA TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA
    AGACAGGACA TCCTTGGCAG GACCTATTAT GTCAATCATG AGTTCAGAAG AACACAGTGG
    AAAAGACCAA CTGCACAGGA CAATGTAGCT GTAGCAGATA TTGGTAACAT GCAGTTGGAG
    GCCCAGCATG CGTTCACTCA TCGGCGGCAG ATATCAGAGG ATACAGAAAA CCTGGACAGC
    AGGGATTCTC CTGAGAGCTG GGAAATTATA ACTGAAGATG AGGCAACATT GTATAGCAAT
    GAGAACCCTC AAATACCTCT ACCACAGAAC AGTTCTGATA TACAGACTCA TCTTGCAGAA
    GAGCTGAATA CCAGACTTAC TACCTCTGGA AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC
    ACTAACCATT CCAGCAGAAG AGGTAGTCTG CAGACATACA GAGTTGAAGA GCAGCCTGCA
    CATCCAGTGT TACTGCCTAC TTCATCTGGA TTACCGCCAG GCTGGGAAGA AAGACAAGAT
    GAAAAAGGGA GATCATATTA CATAGATCAC AATTCCAGAA CTACGACTTG GATAAAACCA
    GTTGTACAGA TTGCTATGGA AGCAGGTCAG TTGCCAGCTG CACAAAATAC TTCTATAGCA
    AGACAGCCAC AAGCAACATC AGGTGATTCA TCACAACAGT CTTCTAATCA GCAGCCTGAA
    ATGGAGCAAG GATTTCTCCC TAAAGGCTGG GAAGTTCGAC ATGCACCTAA TGGGAGACCA
    TTCTTTATTG ACCACAATAC TAAAACTACA ACATGGGAAG ATCCAAGACT GAAAATTCCT
    GCTCATCTGA GGAGAAAGAC TTCCCTAGAT CCAGTAGATC TAGGACCATT ACCACCAGGG
    TGGGAAGAGA GAACACACAC AGACGGAAGA ATATTCTTCA TAAACCACAA TACCAAGAAA
    ACACAATGGG AGGATCCTCG ACTGCAGAAC GTGGCAATAA CCGGACCAGC TGTGCCTTAC
    TCCAGGGACT ATAAGCGGAA GTACGAGTTC TTCAGAAAGA AACTGAAGAA ACAGAGTGAT
    ATTCCAAACA GATTTGAAAT GAAAATTCAT CGCACAACAA TCCTTGAAGA TTCTTACAGA
    AGAATTATAG CTGTGAAGAG AGCAGATTTC CTTAAAGCAA GACTTTGGAT TGAATTTGAT
    GGTGAAAAAG GTTTAGACTA TGGAGGAGTG GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG
    GAGATGTTTA ATCCTTACTA TGGATTGTTC GAATATTCAG CTACAGATAA CTATACTCTG
    CAGATCAACC CAAACTCTGG ACTGTGCAAT GAAGATCATC TCTCTTATTT CAAGTTTATA
    GGTCGAGTAG CAGGAATGGC TGTTTACCAC GGCAAACTTT TGGATGCATT TTTCATTCGT
    CCTTTCTACA AGATGATGCT CCAGAAGCCG ATAACACTTC ATGATATGGA GTCTGTGGAT
    AGTGAATATT ATAATTCTCT GAGATGGATT CTTGAAAATG ATCCAGCGGA ACTGGACCTC
    AGATTTATAG TTGATGAAGA ACTTTTTGGG CAGACTCATC AGCACGAACT GAAAAGTGGT
    GGATCAGAAA TAGTTGTCAC CAACAAAAAC AAGAGAGACT ACATTCATCT TGTAATTCAG
    TGGAGATTTG TGAGCAGAGT ACAGAAACAA ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA
    CTAATACCTC AGGATCTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT ACTGATGTGT
    GGACTGGGAG ATGTGGATGT AGCTGATTGG AAACTACATA CAAAATACAA AAATGGCTAC
    AGTGTGAATC ATCAAGTAAT ACAGTGGTTT TGGAAGGCAG TCTTAATGAT GGACTCTGAA
    AAGAGAATAA GATTACTACA GTTTGTTACT GGCACATCAC GTGTACCTAT GAATGGGTTT
    GCTGAGCTTT ATGGTTCAAA TGGACCACAG TTGTTTACAG TTGAACAGTG GGGTACCCCT
    GAAAAACTGC CGAGAGCTCA TACCTGCTTT AATCGCTTGG ATTTGCCCCC GTATGATTCA
    TTTGAAGATT TATGGGATAA ACTTCTTCTA GCGATTGAAA ATACTCAGGG CTTTGATGGG
    GTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147366.2
    CDS31..2739
    Translation

    Target ORF information:

    RefSeq Version XM_015291880.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCGGCCT CTCCTGTGGA GGTGTTCGGG CTGCCCGGCG AGGAGGAGAA CACACGAATT 
    GTCAGGGTGA AGGTTATAGC TGGAATTGGT CTGGCCAAGA AGGATATCCT AGGAGCCAGT
    GACCCATATG TGAAAGTGAC AGTGTATGAT CCAGTGAATG GAGTCCTTAG CAGTGTTCAG
    ACAAAAACTA TTAGAAAGTC CTTGAACCCA AAGTGGAATG AAGAACTTTT ATTTCGAGTT
    AATCCTCAGA AACACAGACT CCTTTTTGAA GTGTTTGATG AAAACCGTTT GACTCGAGAT
    GATTTTCTTG GGCAAGTGGA TATTCCCCTT TATCAGTTAC CGACAGAACA TCCAAGTATG
    GAGAGACCAT ATACCTTTAG GGATTTTGTT CTTCATCCAA GAAGCCATAA ATCAAGAGTT
    AAAGGTCACC TTAGATTAAA GATGACTTAC TTACCTAAAA GCAATGGATC TGAAGAAGAA
    ACCACAGAAC AGGCCGAGGA ATTGGAGCCT GGGTGGATTT TATTGGACCA ACCGGATGCT
    TCTAACCAGC CACAACAGCA GCAGCAGGAA TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA
    AGACAGGACA TCCTTGGCAG GACCTATTAT GTCAATCATG AGTTCAGAAG AACACAGTGG
    AAAAGACCAA CTGCACAGGA CAATGTAGCT GTAGCAGATA TTGGTAACAT GCAGTTGGAG
    GCCCAGCATG CGTTCACTCA TCGGCGGCAG ATATCAGAGG ATACAGAAAA CCTGGACAGC
    AGGGATTCTC CTGAGAGCTG GGAAATTATA ACTGAAGATG AGGCAACATT GTATAGCAAT
    GAGAACCCTC AAATACCTCT ACCACAGAAC AGTTCTGATA TACAGACTCA TCTTGCAGAA
    GAGCTGAATA CCAGACTTAC TACCTCTGGA AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC
    ACTAACCATT CCAGCAGAAG AGGTAGTCTG CAGACATACA GAGTTGAAGA GCAGCCTGCA
    CATCCAGTGT TACTGCCTAC TTCATCTGGA TTACCGCCAG GCTGGGAAGA AAGACAAGAT
    GAAAAAGGGA GATCATATTA CATAGATCAC AATTCCAGAA CTACGACTTG GATAAAACCA
    GTTGTACAGA TTGCTATGGA AGCAGGTCAG TTGCCAGCTG CACAAAATAC TTCTATAGCA
    AGACAGCCAC AAGCAACATC AGGTGATTCA TCACAACAGT CTTCTAATCA GCAGCCTGAA
    ATGGAGCAAG GATTTCTCCC TAAAGGCTGG GAAGTTCGAC ATGCACCTAA TGGGAGACCA
    TTCTTTATTG ACCACAATAC TAAAACTACA ACATGGGAAG ATCCAAGACT GAAAATTCCT
    GCTCATCTGA GGAGAAAGAC TTCCCTAGAT CCAGTAGATC TAGGACCATT ACCACCAGGG
    TGGGAAGAGA GAACACACAC AGACGGAAGA ATATTCTTCA TAAACCACAA TACCAAGAAA
    ACACAATGGG AGGATCCTCG ACTGCAGAAC GTGGCAATAA CCGGACCAGC TGTGCCTTAC
    TCCAGGGACT ATAAGCGGAA GTACGAGTTC TTCAGAAAGA AACTGAAGAA ACAGAGTGAT
    ATTCCAAACA GATTTGAAAT GAAAATTCAT CGCACAACAA TCCTTGAAGA TTCTTACAGA
    AGAATTATAG CTGTGAAGAG AGCAGATTTC CTTAAAGCAA GACTTTGGAT TGAATTTGAT
    GGTGAAAAAG GTTTAGACTA TGGAGGAGTG GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG
    GAGATGTTTA ATCCTTACTA TGGATTGTTC GAATATTCAG CTACAGATAA CTATACTCTG
    CAGATCAACC CAAACTCTGG ACTGTGCAAT GAAGATCATC TCTCTTATTT CAAGTTTATA
    GGTCGAGTAG CAGGAATGGC TGTTTACCAC GGCAAACTTT TGGATGCATT TTTCATTCGT
    CCTTTCTACA AGATGATGCT CCAGAAGCCG ATAACACTTC ATGATATGGA GTCTGTGGAT
    AGTGAATATT ATAATTCTCT GAGATGGATT CTTGAAAATG ATCCAGCGGA ACTGGACCTC
    AGATTTATAG TTGATGAAGA ACTTTTTGGG CAGACTCATC AGCACGAACT GAAAAGTGGT
    GGATCAGAAA TAGTTGTCAC CAACAAAAAC AAGAGAGACT ACATTCATCT TGTAATTCAG
    TGGAGATTTG TGAGCAGAGT ACAGAAACAA ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA
    CTAATACCTC AGGATCTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT ACTGATGTGT
    GGACTGGGAG ATGTGGATGT AGCTGATTGG AAACTACATA CAAAATACAA AAATGGCTAC
    AGTGTGAATC ATCAAGTAAT ACAGTGGTTT TGGAAGGCAG TCTTAATGAT GGACTCTGAA
    AAGAGAATAA GATTACTACA GTTTGTTACT GGCACATCAC GTGTACCTAT GAATGGGTTT
    GCTGAGCTTT ATGGTTCAAA TGGACCACAG TTGTTTACAG TTGAACAGTG GGGTACCCCT
    GAAAAACTGC CGAGAGCTCA TACCTGCTTT AATCGCTTGG ATTTGCCCCC GTATGATTCA
    TTTGAAGATT TATGGGATAA ACTTCTTCTA GCGATTGAAA ATACTCAGGG CTTTGATGGG
    GTTGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143449
    Clone ID Related Accession (Same CDS sequence) XM_015291881.2
    Accession Version XM_015291881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACCTT TAGGGATTTT GTTCTTCATC CAAGAAGCCA TAAATCAAGA 
    GTTAAAGGTC ACCTTAGATT AAAGATGACT TACTTACCTA AAAGCAATGG ATCTGAAGAA
    GAAACCACAG AACAGGCCGA GGAATTGGAG CCTGGGTGGA TTTTATTGGA CCAACCGGAT
    GCTTCTAACC AGCCACAACA GCAGCAGCAG GAATCTCCTC CACTGCCTTC AGGCTGGGAA
    GAAAGACAGG ACATCCTTGG CAGGACCTAT TATGTCAATC ATGAGTTCAG AAGAACACAG
    TGGAAAAGAC CAACTGCACA GGACAATGTA GCTGTAGCAG ATATTGGTAA CATGCAGTTG
    GAGGCCCAGC ATGCGTTCAC TCATCGGCGG CAGATATCAG AGGATACAGA AAACCTGGAC
    AGCAGGGATT CTCCTGAGAG CTGGGAAATT ATAACTGAAG ATGAGGCAAC ATTGTATAGC
    AATGAGAACC CTCAAATACC TCTACCACAG AACAGTTCTG ATATACAGAC TCATCTTGCA
    GAAGAGCTGA ATACCAGACT TACTACCTCT GGAAGTTCAG CTACTGGCCA GTCAGCAGCC
    TACACTAACC ATTCCAGCAG AAGAGGTAGT CTGCAGACAT ACAGAGTTGA AGAGCAGCCT
    GCACATCCAG TGTTACTGCC TACTTCATCT GGATTACCGC CAGGCTGGGA AGAAAGACAA
    GATGAAAAAG GGAGATCATA TTACATAGAT CACAATTCCA GAACTACGAC TTGGATAAAA
    CCAGTTGTAC AGATTGCTAT GGAAGCAGGT CAGTTGCCAG CTGCACAAAA TACTTCTATA
    GCAAGACAGC CACAAGCAAC ATCAGGTGAT TCATCACAAC AGTCTTCTAA TCAGCAGCCT
    GAAATGGAGC AAGGATTTCT CCCTAAAGGC TGGGAAGTTC GACATGCACC TAATGGGAGA
    CCATTCTTTA TTGACCACAA TACTAAAACT ACAACATGGG AAGATCCAAG ACTGAAAATT
    CCTGCTCATC TGAGGAGAAA GACTTCCCTA GATCCAGTAG ATCTAGGACC ATTACCACCA
    GGGTGGGAAG AGAGAACACA CACAGACGGA AGAATATTCT TCATAAACCA CAATACCAAG
    AAAACACAAT GGGAGGATCC TCGACTGCAG AACGTGGCAA TAACCGGACC AGCTGTGCCT
    TACTCCAGGG ACTATAAGCG GAAGTACGAG TTCTTCAGAA AGAAACTGAA GAAACAGAGT
    GATATTCCAA ACAGATTTGA AATGAAAATT CATCGCACAA CAATCCTTGA AGATTCTTAC
    AGAAGAATTA TAGCTGTGAA GAGAGCAGAT TTCCTTAAAG CAAGACTTTG GATTGAATTT
    GATGGTGAAA AAGGTTTAGA CTATGGAGGA GTGGCCAGGG AATGGTTCTT CCTTCTCTCT
    AAGGAGATGT TTAATCCTTA CTATGGATTG TTCGAATATT CAGCTACAGA TAACTATACT
    CTGCAGATCA ACCCAAACTC TGGACTGTGC AATGAAGATC ATCTCTCTTA TTTCAAGTTT
    ATAGGTCGAG TAGCAGGAAT GGCTGTTTAC CACGGCAAAC TTTTGGATGC ATTTTTCATT
    CGTCCTTTCT ACAAGATGAT GCTCCAGAAG CCGATAACAC TTCATGATAT GGAGTCTGTG
    GATAGTGAAT ATTATAATTC TCTGAGATGG ATTCTTGAAA ATGATCCAGC GGAACTGGAC
    CTCAGATTTA TAGTTGATGA AGAACTTTTT GGGCAGACTC ATCAGCACGA ACTGAAAAGT
    GGTGGATCAG AAATAGTTGT CACCAACAAA AACAAGAGAG ACTACATTCA TCTTGTAATT
    CAGTGGAGAT TTGTGAGCAG AGTACAGAAA CAAATGGCAG CCTTTAAAGA GGGCTTTTTT
    GAACTAATAC CTCAGGATCT GATTAAAATT TTTGATGAAA ATGAGCTGGA GTTACTGATG
    TGTGGACTGG GAGATGTGGA TGTAGCTGAT TGGAAACTAC ATACAAAATA CAAAAATGGC
    TACAGTGTGA ATCATCAAGT AATACAGTGG TTTTGGAAGG CAGTCTTAAT GATGGACTCT
    GAAAAGAGAA TAAGATTACT ACAGTTTGTT ACTGGCACAT CACGTGTACC TATGAATGGG
    TTTGCTGAGC TTTATGGTTC AAATGGACCA CAGTTGTTTA CAGTTGAACA GTGGGGTACC
    CCTGAAAAAC TGCCGAGAGC TCATACCTGC TTTAATCGCT TGGATTTGCC CCCGTATGAT
    TCATTTGAAG ATTTATGGGA TAAACTTCTT CTAGCGATTG AAAATACTCA GGGCTTTGAT
    GGGGTTGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147367.2
    CDS309..2660
    Translation

    Target ORF information:

    RefSeq Version XM_015291881.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGAGAC CATATACCTT TAGGGATTTT GTTCTTCATC CAAGAAGCCA TAAATCAAGA 
    GTTAAAGGTC ACCTTAGATT AAAGATGACT TACTTACCTA AAAGCAATGG ATCTGAAGAA
    GAAACCACAG AACAGGCCGA GGAATTGGAG CCTGGGTGGA TTTTATTGGA CCAACCGGAT
    GCTTCTAACC AGCCACAACA GCAGCAGCAG GAATCTCCTC CACTGCCTTC AGGCTGGGAA
    GAAAGACAGG ACATCCTTGG CAGGACCTAT TATGTCAATC ATGAGTTCAG AAGAACACAG
    TGGAAAAGAC CAACTGCACA GGACAATGTA GCTGTAGCAG ATATTGGTAA CATGCAGTTG
    GAGGCCCAGC ATGCGTTCAC TCATCGGCGG CAGATATCAG AGGATACAGA AAACCTGGAC
    AGCAGGGATT CTCCTGAGAG CTGGGAAATT ATAACTGAAG ATGAGGCAAC ATTGTATAGC
    AATGAGAACC CTCAAATACC TCTACCACAG AACAGTTCTG ATATACAGAC TCATCTTGCA
    GAAGAGCTGA ATACCAGACT TACTACCTCT GGAAGTTCAG CTACTGGCCA GTCAGCAGCC
    TACACTAACC ATTCCAGCAG AAGAGGTAGT CTGCAGACAT ACAGAGTTGA AGAGCAGCCT
    GCACATCCAG TGTTACTGCC TACTTCATCT GGATTACCGC CAGGCTGGGA AGAAAGACAA
    GATGAAAAAG GGAGATCATA TTACATAGAT CACAATTCCA GAACTACGAC TTGGATAAAA
    CCAGTTGTAC AGATTGCTAT GGAAGCAGGT CAGTTGCCAG CTGCACAAAA TACTTCTATA
    GCAAGACAGC CACAAGCAAC ATCAGGTGAT TCATCACAAC AGTCTTCTAA TCAGCAGCCT
    GAAATGGAGC AAGGATTTCT CCCTAAAGGC TGGGAAGTTC GACATGCACC TAATGGGAGA
    CCATTCTTTA TTGACCACAA TACTAAAACT ACAACATGGG AAGATCCAAG ACTGAAAATT
    CCTGCTCATC TGAGGAGAAA GACTTCCCTA GATCCAGTAG ATCTAGGACC ATTACCACCA
    GGGTGGGAAG AGAGAACACA CACAGACGGA AGAATATTCT TCATAAACCA CAATACCAAG
    AAAACACAAT GGGAGGATCC TCGACTGCAG AACGTGGCAA TAACCGGACC AGCTGTGCCT
    TACTCCAGGG ACTATAAGCG GAAGTACGAG TTCTTCAGAA AGAAACTGAA GAAACAGAGT
    GATATTCCAA ACAGATTTGA AATGAAAATT CATCGCACAA CAATCCTTGA AGATTCTTAC
    AGAAGAATTA TAGCTGTGAA GAGAGCAGAT TTCCTTAAAG CAAGACTTTG GATTGAATTT
    GATGGTGAAA AAGGTTTAGA CTATGGAGGA GTGGCCAGGG AATGGTTCTT CCTTCTCTCT
    AAGGAGATGT TTAATCCTTA CTATGGATTG TTCGAATATT CAGCTACAGA TAACTATACT
    CTGCAGATCA ACCCAAACTC TGGACTGTGC AATGAAGATC ATCTCTCTTA TTTCAAGTTT
    ATAGGTCGAG TAGCAGGAAT GGCTGTTTAC CACGGCAAAC TTTTGGATGC ATTTTTCATT
    CGTCCTTTCT ACAAGATGAT GCTCCAGAAG CCGATAACAC TTCATGATAT GGAGTCTGTG
    GATAGTGAAT ATTATAATTC TCTGAGATGG ATTCTTGAAA ATGATCCAGC GGAACTGGAC
    CTCAGATTTA TAGTTGATGA AGAACTTTTT GGGCAGACTC ATCAGCACGA ACTGAAAAGT
    GGTGGATCAG AAATAGTTGT CACCAACAAA AACAAGAGAG ACTACATTCA TCTTGTAATT
    CAGTGGAGAT TTGTGAGCAG AGTACAGAAA CAAATGGCAG CCTTTAAAGA GGGCTTTTTT
    GAACTAATAC CTCAGGATCT GATTAAAATT TTTGATGAAA ATGAGCTGGA GTTACTGATG
    TGTGGACTGG GAGATGTGGA TGTAGCTGAT TGGAAACTAC ATACAAAATA CAAAAATGGC
    TACAGTGTGA ATCATCAAGT AATACAGTGG TTTTGGAAGG CAGTCTTAAT GATGGACTCT
    GAAAAGAGAA TAAGATTACT ACAGTTTGTT ACTGGCACAT CACGTGTACC TATGAATGGG
    TTTGCTGAGC TTTATGGTTC AAATGGACCA CAGTTGTTTA CAGTTGAACA GTGGGGTACC
    CCTGAAAAAC TGCCGAGAGC TCATACCTGC TTTAATCGCT TGGATTTGCC CCCGTATGAT
    TCATTTGAAG ATTTATGGGA TAAACTTCTT CTAGCGATTG AAAATACTCA GGGCTTTGAT
    GGGGTTGATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40464
    Clone ID Related Accession (Same CDS sequence) XM_015291878.1
    Accession Version XM_015291878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2679bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTGTG TTTGGGAATC TGAATTTCAG TGCCTGAATC TAGCGGTTCT TCATTGCTCT 
    GCTCCAGTTT TCTGTAAGAT GGGTCTCTCT CTGTTACTCA CAGGACTTCT TGTGACTCTG
    CAGGATATTG AAATGGAGAA CACACGAATT GTCAGGGTGA AGGTTATAGC TGGAATTGGT
    CTGGCCAAGA AGGATATCCT AGGAGCCAGT GACCCATATG TGAAAGTGAC AGTGTATGAT
    CCAGTGAATG GAGTCCTTAG CAGTGTTCAG ACAAAAACTA TTAGAAAGTC CTTGAACCCA
    AAGTGGAATG AAGAACTTTT ATTTCGAGTT AATCCTCAGA AACACAGACT CCTTTTTGAA
    GTGTTTGATG AAAACCGTTT GACTCGAGAT GATTTTCTTG GGCAAGTGGA TATTCCCCTT
    TATCAGTTAC CGACAGAACA TCCAAGTATG GAGAGACCAT ATACCTTTAG GGATTTTGTT
    CTTCATCCAA GAAGCCATAA ATCAAGAGTT AAAGGTCACC TTAGATTAAA GATGACTTAC
    TTACCTAAAA GCAATGGATC TGAAGAAGAA ACCACAGAAC AGGCCGAGGA ATTGGAGCCT
    GGGTGGATTT TATTGGACCA ACCGGATGCT TCTAACCAGC CACAACAGCA GCAGCAGGAA
    TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA AGACAGGACA TCCTTGGCAG GACCTATTAT
    GTCAATCATG AGTTCAGAAG AACACAGTGG AAAAGACCAA CTGCACAGGA CAATGTAGCT
    GTAGCAGATA TTGGTAACAT GCAGTTGGAG GCCCAGCATG CGTTCACTCA TCGGCGGCAG
    ATATCAGAGG ATACAGAAAA CCTGGACAGC AGGGATTCTC CTGAGAGCTG GGAAATTATA
    ACTGAAGATG AGGCAACATT GTATAGCAAT GAGAACCCTC AAATACCTCT ACCACAGAAC
    AGTTCTGATA TACAGACTCA TCTTGCAGAA GAGCTGAATA CCAGACTTAC TACCTCTGGA
    AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC ACTAACCATT CCAGCAGAAG AGGTAGTCTG
    CAGACATACA GAGTTGAAGA GCAGCCTGCA CATCCAGTGT TACTGCCTAC TTCATCTGGA
    TTACCGCCAG GCTGGGAAGA AAGACAAGAT GAAAAAGGGA GGTCATATTA CATAGATCAC
    AATTCCAGAA CTACGACTTG GATAAAACCA GTTGTACAGA TTGCTATGGA AGCAGGTCAG
    TTGCCAGCTG CACAAAATAC TTCTATAGCA AGACAGCCAC AAGCAACATC AGGTGATTCA
    TCACAACAGT CTTCTAATCA GCAGCCTGAA ATGGAGCAAG GATTTCTCCC TAAAGGCTGG
    GAAGTTCGAC ATGCACCTAA TGGGAGACCA TTCTTTATTG ACCACAATAC TAAAACTACA
    ACATGGGAAG ATCCAAGACT GAAAATTCCT GCTCATCTGA GGAGAAAGAC TTCCCTAGAT
    CCAGTAGATC TAGGACCATT ACCACCAGGG TGGGAAGAGA GAACACACAC AGACGGAAGA
    ATATTCTTCA TAAACCACAA TACCAAGAAA ACACAATGGG AGGATCCTCG ACTGCAGAAC
    GTGGCAATAA CCGGACCAGC TGTGCCTTAC TCCAGGGACT ATAAGCGGAA GTACGAGTTC
    TTCAGAAAGA AACTGAAGAA ACAGAGTGAT ATTCCAAACA GATTTGAAAT GAAAATTCAT
    CGCACAACAA TCCTTGAAGA TTCTTACAGA AGAATTATAG CTGTGAAGAG AGCAGATTTC
    CTTAAAGCAA GACTTTGGAT TGAATTTGAT GGTGAAAAAG GTTTAGACTA TGGAGGAGTG
    GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG GAGATGTTTA ATCCTTACTA TGGATTGTTC
    GAATATTCAG CTACAGATAA CTATACTCTG CAGATCAACC CAAACTCTGG ACTGTGCAAT
    GAAGATCATC TCTCTTATTT CAAGTTTATA GGTCGAGTAG CAGGAATGGC TGTTTACCAC
    GGCAAACTTT TGGATGCATT TTTCATTCGT CCTTTCTACA AGATGATGCT CCAGAAGCCG
    ATAACACTTC ATGATATGGA GTCTGTGGAT AGTGAATATT ATAATTCTCT GAGATGGATT
    CTTGAAAATG ATCCAGCGGA ACTGGACCTC AGATTTATAG TTGATGAAGA ACTTTTTGGG
    CAGACTCATC AGCACGAACT GAAAAGTGGT GGATCAGAAA TAGTTGTCAC CAACAAAAAC
    AAGAGAGACT ACATTCATCT TGTAATTCAG TGGAGATTTG TGAGCAGAGT ACAGAAACAA
    ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA CTAATACCTC AGGATCTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT ACTGATGTGT GGACTGGGAG ATGTGGATGT AGCTGATTGG
    AAACTACATA CAAAATACAA AAATGGCTAC AGTGTGAATC ATCAAGTAAT ACAGTGGTTT
    TGGAAGGCAG TCTTAATGAT GGACTCTGAA AAGAGAATAA GATTACTACA GTTTGTTACT
    GGCACATCAC GTGTACCTAT GAATGGGTTT GCTGAGCTTT ATGGTGAGTA CATAAGATTG
    TATTCACAAT TAACTGAGAA CATGATACAG CTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147364.1
    CDS397..3075
    Translation

    Target ORF information:

    RefSeq Version XM_015291878.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTGTTGTG TTTGGGAATC TGAATTTCAG TGCCTGAATC TAGCGGTTCT TCATTGCTCT 
    GCTCCAGTTT TCTGTAAGAT GGGTCTCTCT CTGTTACTCA CAGGACTTCT TGTGACTCTG
    CAGGATATTG AAATGGAGAA CACACGAATT GTCAGGGTGA AGGTTATAGC TGGAATTGGT
    CTGGCCAAGA AGGATATCCT AGGAGCCAGT GACCCATATG TGAAAGTGAC AGTGTATGAT
    CCAGTGAATG GAGTCCTTAG CAGTGTTCAG ACAAAAACTA TTAGAAAGTC CTTGAACCCA
    AAGTGGAATG AAGAACTTTT ATTTCGAGTT AATCCTCAGA AACACAGACT CCTTTTTGAA
    GTGTTTGATG AAAACCGTTT GACTCGAGAT GATTTTCTTG GGCAAGTGGA TATTCCCCTT
    TATCAGTTAC CGACAGAACA TCCAAGTATG GAGAGACCAT ATACCTTTAG GGATTTTGTT
    CTTCATCCAA GAAGCCATAA ATCAAGAGTT AAAGGTCACC TTAGATTAAA GATGACTTAC
    TTACCTAAAA GCAATGGATC TGAAGAAGAA ACCACAGAAC AGGCCGAGGA ATTGGAGCCT
    GGGTGGATTT TATTGGACCA ACCGGATGCT TCTAACCAGC CACAACAGCA GCAGCAGGAA
    TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA AGACAGGACA TCCTTGGCAG GACCTATTAT
    GTCAATCATG AGTTCAGAAG AACACAGTGG AAAAGACCAA CTGCACAGGA CAATGTAGCT
    GTAGCAGATA TTGGTAACAT GCAGTTGGAG GCCCAGCATG CGTTCACTCA TCGGCGGCAG
    ATATCAGAGG ATACAGAAAA CCTGGACAGC AGGGATTCTC CTGAGAGCTG GGAAATTATA
    ACTGAAGATG AGGCAACATT GTATAGCAAT GAGAACCCTC AAATACCTCT ACCACAGAAC
    AGTTCTGATA TACAGACTCA TCTTGCAGAA GAGCTGAATA CCAGACTTAC TACCTCTGGA
    AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC ACTAACCATT CCAGCAGAAG AGGTAGTCTG
    CAGACATACA GAGTTGAAGA GCAGCCTGCA CATCCAGTGT TACTGCCTAC TTCATCTGGA
    TTACCGCCAG GCTGGGAAGA AAGACAAGAT GAAAAAGGGA GGTCATATTA CATAGATCAC
    AATTCCAGAA CTACGACTTG GATAAAACCA GTTGTACAGA TTGCTATGGA AGCAGGTCAG
    TTGCCAGCTG CACAAAATAC TTCTATAGCA AGACAGCCAC AAGCAACATC AGGTGATTCA
    TCACAACAGT CTTCTAATCA GCAGCCTGAA ATGGAGCAAG GATTTCTCCC TAAAGGCTGG
    GAAGTTCGAC ATGCACCTAA TGGGAGACCA TTCTTTATTG ACCACAATAC TAAAACTACA
    ACATGGGAAG ATCCAAGACT GAAAATTCCT GCTCATCTGA GGAGAAAGAC TTCCCTAGAT
    CCAGTAGATC TAGGACCATT ACCACCAGGG TGGGAAGAGA GAACACACAC AGACGGAAGA
    ATATTCTTCA TAAACCACAA TACCAAGAAA ACACAATGGG AGGATCCTCG ACTGCAGAAC
    GTGGCAATAA CCGGACCAGC TGTGCCTTAC TCCAGGGACT ATAAGCGGAA GTACGAGTTC
    TTCAGAAAGA AACTGAAGAA ACAGAGTGAT ATTCCAAACA GATTTGAAAT GAAAATTCAT
    CGCACAACAA TCCTTGAAGA TTCTTACAGA AGAATTATAG CTGTGAAGAG AGCAGATTTC
    CTTAAAGCAA GACTTTGGAT TGAATTTGAT GGTGAAAAAG GTTTAGACTA TGGAGGAGTG
    GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG GAGATGTTTA ATCCTTACTA TGGATTGTTC
    GAATATTCAG CTACAGATAA CTATACTCTG CAGATCAACC CAAACTCTGG ACTGTGCAAT
    GAAGATCATC TCTCTTATTT CAAGTTTATA GGTCGAGTAG CAGGAATGGC TGTTTACCAC
    GGCAAACTTT TGGATGCATT TTTCATTCGT CCTTTCTACA AGATGATGCT CCAGAAGCCG
    ATAACACTTC ATGATATGGA GTCTGTGGAT AGTGAATATT ATAATTCTCT GAGATGGATT
    CTTGAAAATG ATCCAGCGGA ACTGGACCTC AGATTTATAG TTGATGAAGA ACTTTTTGGG
    CAGACTCATC AGCACGAACT GAAAAGTGGT GGATCAGAAA TAGTTGTCAC CAACAAAAAC
    AAGAGAGACT ACATTCATCT TGTAATTCAG TGGAGATTTG TGAGCAGAGT ACAGAAACAA
    ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA CTAATACCTC AGGATCTGAT TAAAATTTTT
    GATGAAAATG AGCTGGAGTT ACTGATGTGT GGACTGGGAG ATGTGGATGT AGCTGATTGG
    AAACTACATA CAAAATACAA AAATGGCTAC AGTGTGAATC ATCAAGTAAT ACAGTGGTTT
    TGGAAGGCAG TCTTAATGAT GGACTCTGAA AAGAGAATAA GATTACTACA GTTTGTTACT
    GGCACATCAC GTGTACCTAT GAATGGGTTT GCTGAGCTTT ATGGTGAGTA CATAAGATTG
    TATTCACAAT TAACTGAGAA CATGATACAG CTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40465
    Clone ID Related Accession (Same CDS sequence) XM_015291879.1
    Accession Version XM_015291879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2607bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAGGGT CGGCTGCATC CTGGAATTGG TACAAAGGAG ATGATACCAG GATTTGGGAG 
    CAGGAGAACA CACGAATTGT CAGGGTGAAG GTTATAGCTG GAATTGGTCT GGCCAAGAAG
    GATATCCTAG GAGCCAGTGA CCCATATGTG AAAGTGACAG TGTATGATCC AGTGAATGGA
    GTCCTTAGCA GTGTTCAGAC AAAAACTATT AGAAAGTCCT TGAACCCAAA GTGGAATGAA
    GAACTTTTAT TTCGAGTTAA TCCTCAGAAA CACAGACTCC TTTTTGAAGT GTTTGATGAA
    AACCGTTTGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGG TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTGAGTACA TAAGATTGTA TTCACAATTA
    ACTGAGAACA TGATACAGCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147365.1
    CDS89..2695
    Translation

    Target ORF information:

    RefSeq Version XM_015291879.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAAGGGT CGGCTGCATC CTGGAATTGG TACAAAGGAG ATGATACCAG GATTTGGGAG 
    CAGGAGAACA CACGAATTGT CAGGGTGAAG GTTATAGCTG GAATTGGTCT GGCCAAGAAG
    GATATCCTAG GAGCCAGTGA CCCATATGTG AAAGTGACAG TGTATGATCC AGTGAATGGA
    GTCCTTAGCA GTGTTCAGAC AAAAACTATT AGAAAGTCCT TGAACCCAAA GTGGAATGAA
    GAACTTTTAT TTCGAGTTAA TCCTCAGAAA CACAGACTCC TTTTTGAAGT GTTTGATGAA
    AACCGTTTGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGG TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTGAGTACA TAAGATTGTA TTCACAATTA
    ACTGAGAACA TGATACAGCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40466
    Clone ID Related Accession (Same CDS sequence) XM_015291880.1
    Accession Version XM_015291880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455921.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCGGCCT CTCCTGTGGA GGTGTTCGGG CTGCCCGGCG AGGAGGAGAA CACACGAATT 
    GTCAGGGTGA AGGTTATAGC TGGAATTGGT CTGGCCAAGA AGGATATCCT AGGAGCCAGT
    GACCCATATG TGAAAGTGAC AGTGTATGAT CCAGTGAATG GAGTCCTTAG CAGTGTTCAG
    ACAAAAACTA TTAGAAAGTC CTTGAACCCA AAGTGGAATG AAGAACTTTT ATTTCGAGTT
    AATCCTCAGA AACACAGACT CCTTTTTGAA GTGTTTGATG AAAACCGTTT GACTCGAGAT
    GATTTTCTTG GGCAAGTGGA TATTCCCCTT TATCAGTTAC CGACAGAACA TCCAAGTATG
    GAGAGACCAT ATACCTTTAG GGATTTTGTT CTTCATCCAA GAAGCCATAA ATCAAGAGTT
    AAAGGTCACC TTAGATTAAA GATGACTTAC TTACCTAAAA GCAATGGATC TGAAGAAGAA
    ACCACAGAAC AGGCCGAGGA ATTGGAGCCT GGGTGGATTT TATTGGACCA ACCGGATGCT
    TCTAACCAGC CACAACAGCA GCAGCAGGAA TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA
    AGACAGGACA TCCTTGGCAG GACCTATTAT GTCAATCATG AGTTCAGAAG AACACAGTGG
    AAAAGACCAA CTGCACAGGA CAATGTAGCT GTAGCAGATA TTGGTAACAT GCAGTTGGAG
    GCCCAGCATG CGTTCACTCA TCGGCGGCAG ATATCAGAGG ATACAGAAAA CCTGGACAGC
    AGGGATTCTC CTGAGAGCTG GGAAATTATA ACTGAAGATG AGGCAACATT GTATAGCAAT
    GAGAACCCTC AAATACCTCT ACCACAGAAC AGTTCTGATA TACAGACTCA TCTTGCAGAA
    GAGCTGAATA CCAGACTTAC TACCTCTGGA AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC
    ACTAACCATT CCAGCAGAAG AGGTAGTCTG CAGACATACA GAGTTGAAGA GCAGCCTGCA
    CATCCAGTGT TACTGCCTAC TTCATCTGGA TTACCGCCAG GCTGGGAAGA AAGACAAGAT
    GAAAAAGGGA GGTCATATTA CATAGATCAC AATTCCAGAA CTACGACTTG GATAAAACCA
    GTTGTACAGA TTGCTATGGA AGCAGGTCAG TTGCCAGCTG CACAAAATAC TTCTATAGCA
    AGACAGCCAC AAGCAACATC AGGTGATTCA TCACAACAGT CTTCTAATCA GCAGCCTGAA
    ATGGAGCAAG GATTTCTCCC TAAAGGCTGG GAAGTTCGAC ATGCACCTAA TGGGAGACCA
    TTCTTTATTG ACCACAATAC TAAAACTACA ACATGGGAAG ATCCAAGACT GAAAATTCCT
    GCTCATCTGA GGAGAAAGAC TTCCCTAGAT CCAGTAGATC TAGGACCATT ACCACCAGGG
    TGGGAAGAGA GAACACACAC AGACGGAAGA ATATTCTTCA TAAACCACAA TACCAAGAAA
    ACACAATGGG AGGATCCTCG ACTGCAGAAC GTGGCAATAA CCGGACCAGC TGTGCCTTAC
    TCCAGGGACT ATAAGCGGAA GTACGAGTTC TTCAGAAAGA AACTGAAGAA ACAGAGTGAT
    ATTCCAAACA GATTTGAAAT GAAAATTCAT CGCACAACAA TCCTTGAAGA TTCTTACAGA
    AGAATTATAG CTGTGAAGAG AGCAGATTTC CTTAAAGCAA GACTTTGGAT TGAATTTGAT
    GGTGAAAAAG GTTTAGACTA TGGAGGAGTG GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG
    GAGATGTTTA ATCCTTACTA TGGATTGTTC GAATATTCAG CTACAGATAA CTATACTCTG
    CAGATCAACC CAAACTCTGG ACTGTGCAAT GAAGATCATC TCTCTTATTT CAAGTTTATA
    GGTCGAGTAG CAGGAATGGC TGTTTACCAC GGCAAACTTT TGGATGCATT TTTCATTCGT
    CCTTTCTACA AGATGATGCT CCAGAAGCCG ATAACACTTC ATGATATGGA GTCTGTGGAT
    AGTGAATATT ATAATTCTCT GAGATGGATT CTTGAAAATG ATCCAGCGGA ACTGGACCTC
    AGATTTATAG TTGATGAAGA ACTTTTTGGG CAGACTCATC AGCACGAACT GAAAAGTGGT
    GGATCAGAAA TAGTTGTCAC CAACAAAAAC AAGAGAGACT ACATTCATCT TGTAATTCAG
    TGGAGATTTG TGAGCAGAGT ACAGAAACAA ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA
    CTAATACCTC AGGATCTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT ACTGATGTGT
    GGACTGGGAG ATGTGGATGT AGCTGATTGG AAACTACATA CAAAATACAA AAATGGCTAC
    AGTGTGAATC ATCAAGTAAT ACAGTGGTTT TGGAAGGCAG TCTTAATGAT GGACTCTGAA
    AAGAGAATAA GATTACTACA GTTTGTTACT GGCACATCAC GTGTACCTAT GAATGGGTTT
    GCTGAGCTTT ATGGTGAGTA CATAAGATTG TATTCACAAT TAACTGAGAA CATGATACAG
    CTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147366.1
    CDS31..2619
    Translation

    Target ORF information:

    RefSeq Version XM_015291880.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCGGCCT CTCCTGTGGA GGTGTTCGGG CTGCCCGGCG AGGAGGAGAA CACACGAATT 
    GTCAGGGTGA AGGTTATAGC TGGAATTGGT CTGGCCAAGA AGGATATCCT AGGAGCCAGT
    GACCCATATG TGAAAGTGAC AGTGTATGAT CCAGTGAATG GAGTCCTTAG CAGTGTTCAG
    ACAAAAACTA TTAGAAAGTC CTTGAACCCA AAGTGGAATG AAGAACTTTT ATTTCGAGTT
    AATCCTCAGA AACACAGACT CCTTTTTGAA GTGTTTGATG AAAACCGTTT GACTCGAGAT
    GATTTTCTTG GGCAAGTGGA TATTCCCCTT TATCAGTTAC CGACAGAACA TCCAAGTATG
    GAGAGACCAT ATACCTTTAG GGATTTTGTT CTTCATCCAA GAAGCCATAA ATCAAGAGTT
    AAAGGTCACC TTAGATTAAA GATGACTTAC TTACCTAAAA GCAATGGATC TGAAGAAGAA
    ACCACAGAAC AGGCCGAGGA ATTGGAGCCT GGGTGGATTT TATTGGACCA ACCGGATGCT
    TCTAACCAGC CACAACAGCA GCAGCAGGAA TCTCCTCCAC TGCCTTCAGG CTGGGAAGAA
    AGACAGGACA TCCTTGGCAG GACCTATTAT GTCAATCATG AGTTCAGAAG AACACAGTGG
    AAAAGACCAA CTGCACAGGA CAATGTAGCT GTAGCAGATA TTGGTAACAT GCAGTTGGAG
    GCCCAGCATG CGTTCACTCA TCGGCGGCAG ATATCAGAGG ATACAGAAAA CCTGGACAGC
    AGGGATTCTC CTGAGAGCTG GGAAATTATA ACTGAAGATG AGGCAACATT GTATAGCAAT
    GAGAACCCTC AAATACCTCT ACCACAGAAC AGTTCTGATA TACAGACTCA TCTTGCAGAA
    GAGCTGAATA CCAGACTTAC TACCTCTGGA AGTTCAGCTA CTGGCCAGTC AGCAGCCTAC
    ACTAACCATT CCAGCAGAAG AGGTAGTCTG CAGACATACA GAGTTGAAGA GCAGCCTGCA
    CATCCAGTGT TACTGCCTAC TTCATCTGGA TTACCGCCAG GCTGGGAAGA AAGACAAGAT
    GAAAAAGGGA GGTCATATTA CATAGATCAC AATTCCAGAA CTACGACTTG GATAAAACCA
    GTTGTACAGA TTGCTATGGA AGCAGGTCAG TTGCCAGCTG CACAAAATAC TTCTATAGCA
    AGACAGCCAC AAGCAACATC AGGTGATTCA TCACAACAGT CTTCTAATCA GCAGCCTGAA
    ATGGAGCAAG GATTTCTCCC TAAAGGCTGG GAAGTTCGAC ATGCACCTAA TGGGAGACCA
    TTCTTTATTG ACCACAATAC TAAAACTACA ACATGGGAAG ATCCAAGACT GAAAATTCCT
    GCTCATCTGA GGAGAAAGAC TTCCCTAGAT CCAGTAGATC TAGGACCATT ACCACCAGGG
    TGGGAAGAGA GAACACACAC AGACGGAAGA ATATTCTTCA TAAACCACAA TACCAAGAAA
    ACACAATGGG AGGATCCTCG ACTGCAGAAC GTGGCAATAA CCGGACCAGC TGTGCCTTAC
    TCCAGGGACT ATAAGCGGAA GTACGAGTTC TTCAGAAAGA AACTGAAGAA ACAGAGTGAT
    ATTCCAAACA GATTTGAAAT GAAAATTCAT CGCACAACAA TCCTTGAAGA TTCTTACAGA
    AGAATTATAG CTGTGAAGAG AGCAGATTTC CTTAAAGCAA GACTTTGGAT TGAATTTGAT
    GGTGAAAAAG GTTTAGACTA TGGAGGAGTG GCCAGGGAAT GGTTCTTCCT TCTCTCTAAG
    GAGATGTTTA ATCCTTACTA TGGATTGTTC GAATATTCAG CTACAGATAA CTATACTCTG
    CAGATCAACC CAAACTCTGG ACTGTGCAAT GAAGATCATC TCTCTTATTT CAAGTTTATA
    GGTCGAGTAG CAGGAATGGC TGTTTACCAC GGCAAACTTT TGGATGCATT TTTCATTCGT
    CCTTTCTACA AGATGATGCT CCAGAAGCCG ATAACACTTC ATGATATGGA GTCTGTGGAT
    AGTGAATATT ATAATTCTCT GAGATGGATT CTTGAAAATG ATCCAGCGGA ACTGGACCTC
    AGATTTATAG TTGATGAAGA ACTTTTTGGG CAGACTCATC AGCACGAACT GAAAAGTGGT
    GGATCAGAAA TAGTTGTCAC CAACAAAAAC AAGAGAGACT ACATTCATCT TGTAATTCAG
    TGGAGATTTG TGAGCAGAGT ACAGAAACAA ATGGCAGCCT TTAAAGAGGG CTTTTTTGAA
    CTAATACCTC AGGATCTGAT TAAAATTTTT GATGAAAATG AGCTGGAGTT ACTGATGTGT
    GGACTGGGAG ATGTGGATGT AGCTGATTGG AAACTACATA CAAAATACAA AAATGGCTAC
    AGTGTGAATC ATCAAGTAAT ACAGTGGTTT TGGAAGGCAG TCTTAATGAT GGACTCTGAA
    AAGAGAATAA GATTACTACA GTTTGTTACT GGCACATCAC GTGTACCTAT GAATGGGTTT
    GCTGAGCTTT ATGGTGAGTA CATAAGATTG TATTCACAAT TAACTGAGAA CATGATACAG
    CTATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40467
    Clone ID Related Accession (Same CDS sequence) XM_015291881.1
    Accession Version XM_015291881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2232bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGAGAC CATATACCTT TAGGGATTTT GTTCTTCATC CAAGAAGCCA TAAATCAAGA 
    GTTAAAGGTC ACCTTAGATT AAAGATGACT TACTTACCTA AAAGCAATGG ATCTGAAGAA
    GAAACCACAG AACAGGCCGA GGAATTGGAG CCTGGGTGGA TTTTATTGGA CCAACCGGAT
    GCTTCTAACC AGCCACAACA GCAGCAGCAG GAATCTCCTC CACTGCCTTC AGGCTGGGAA
    GAAAGACAGG ACATCCTTGG CAGGACCTAT TATGTCAATC ATGAGTTCAG AAGAACACAG
    TGGAAAAGAC CAACTGCACA GGACAATGTA GCTGTAGCAG ATATTGGTAA CATGCAGTTG
    GAGGCCCAGC ATGCGTTCAC TCATCGGCGG CAGATATCAG AGGATACAGA AAACCTGGAC
    AGCAGGGATT CTCCTGAGAG CTGGGAAATT ATAACTGAAG ATGAGGCAAC ATTGTATAGC
    AATGAGAACC CTCAAATACC TCTACCACAG AACAGTTCTG ATATACAGAC TCATCTTGCA
    GAAGAGCTGA ATACCAGACT TACTACCTCT GGAAGTTCAG CTACTGGCCA GTCAGCAGCC
    TACACTAACC ATTCCAGCAG AAGAGGTAGT CTGCAGACAT ACAGAGTTGA AGAGCAGCCT
    GCACATCCAG TGTTACTGCC TACTTCATCT GGATTACCGC CAGGCTGGGA AGAAAGACAA
    GATGAAAAAG GGAGGTCATA TTACATAGAT CACAATTCCA GAACTACGAC TTGGATAAAA
    CCAGTTGTAC AGATTGCTAT GGAAGCAGGT CAGTTGCCAG CTGCACAAAA TACTTCTATA
    GCAAGACAGC CACAAGCAAC ATCAGGTGAT TCATCACAAC AGTCTTCTAA TCAGCAGCCT
    GAAATGGAGC AAGGATTTCT CCCTAAAGGC TGGGAAGTTC GACATGCACC TAATGGGAGA
    CCATTCTTTA TTGACCACAA TACTAAAACT ACAACATGGG AAGATCCAAG ACTGAAAATT
    CCTGCTCATC TGAGGAGAAA GACTTCCCTA GATCCAGTAG ATCTAGGACC ATTACCACCA
    GGGTGGGAAG AGAGAACACA CACAGACGGA AGAATATTCT TCATAAACCA CAATACCAAG
    AAAACACAAT GGGAGGATCC TCGACTGCAG AACGTGGCAA TAACCGGACC AGCTGTGCCT
    TACTCCAGGG ACTATAAGCG GAAGTACGAG TTCTTCAGAA AGAAACTGAA GAAACAGAGT
    GATATTCCAA ACAGATTTGA AATGAAAATT CATCGCACAA CAATCCTTGA AGATTCTTAC
    AGAAGAATTA TAGCTGTGAA GAGAGCAGAT TTCCTTAAAG CAAGACTTTG GATTGAATTT
    GATGGTGAAA AAGGTTTAGA CTATGGAGGA GTGGCCAGGG AATGGTTCTT CCTTCTCTCT
    AAGGAGATGT TTAATCCTTA CTATGGATTG TTCGAATATT CAGCTACAGA TAACTATACT
    CTGCAGATCA ACCCAAACTC TGGACTGTGC AATGAAGATC ATCTCTCTTA TTTCAAGTTT
    ATAGGTCGAG TAGCAGGAAT GGCTGTTTAC CACGGCAAAC TTTTGGATGC ATTTTTCATT
    CGTCCTTTCT ACAAGATGAT GCTCCAGAAG CCGATAACAC TTCATGATAT GGAGTCTGTG
    GATAGTGAAT ATTATAATTC TCTGAGATGG ATTCTTGAAA ATGATCCAGC GGAACTGGAC
    CTCAGATTTA TAGTTGATGA AGAACTTTTT GGGCAGACTC ATCAGCACGA ACTGAAAAGT
    GGTGGATCAG AAATAGTTGT CACCAACAAA AACAAGAGAG ACTACATTCA TCTTGTAATT
    CAGTGGAGAT TTGTGAGCAG AGTACAGAAA CAAATGGCAG CCTTTAAAGA GGGCTTTTTT
    GAACTAATAC CTCAGGATCT GATTAAAATT TTTGATGAAA ATGAGCTGGA GTTACTGATG
    TGTGGACTGG GAGATGTGGA TGTAGCTGAT TGGAAACTAC ATACAAAATA CAAAAATGGC
    TACAGTGTGA ATCATCAAGT AATACAGTGG TTTTGGAAGG CAGTCTTAAT GATGGACTCT
    GAAAAGAGAA TAAGATTACT ACAGTTTGTT ACTGGCACAT CACGTGTACC TATGAATGGG
    TTTGCTGAGC TTTATGGTGA GTACATAAGA TTGTATTCAC AATTAACTGA GAACATGATA
    CAGCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147367.1
    CDS309..2540
    Translation

    Target ORF information:

    RefSeq Version XM_015291881.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGAGAGAC CATATACCTT TAGGGATTTT GTTCTTCATC CAAGAAGCCA TAAATCAAGA 
    GTTAAAGGTC ACCTTAGATT AAAGATGACT TACTTACCTA AAAGCAATGG ATCTGAAGAA
    GAAACCACAG AACAGGCCGA GGAATTGGAG CCTGGGTGGA TTTTATTGGA CCAACCGGAT
    GCTTCTAACC AGCCACAACA GCAGCAGCAG GAATCTCCTC CACTGCCTTC AGGCTGGGAA
    GAAAGACAGG ACATCCTTGG CAGGACCTAT TATGTCAATC ATGAGTTCAG AAGAACACAG
    TGGAAAAGAC CAACTGCACA GGACAATGTA GCTGTAGCAG ATATTGGTAA CATGCAGTTG
    GAGGCCCAGC ATGCGTTCAC TCATCGGCGG CAGATATCAG AGGATACAGA AAACCTGGAC
    AGCAGGGATT CTCCTGAGAG CTGGGAAATT ATAACTGAAG ATGAGGCAAC ATTGTATAGC
    AATGAGAACC CTCAAATACC TCTACCACAG AACAGTTCTG ATATACAGAC TCATCTTGCA
    GAAGAGCTGA ATACCAGACT TACTACCTCT GGAAGTTCAG CTACTGGCCA GTCAGCAGCC
    TACACTAACC ATTCCAGCAG AAGAGGTAGT CTGCAGACAT ACAGAGTTGA AGAGCAGCCT
    GCACATCCAG TGTTACTGCC TACTTCATCT GGATTACCGC CAGGCTGGGA AGAAAGACAA
    GATGAAAAAG GGAGGTCATA TTACATAGAT CACAATTCCA GAACTACGAC TTGGATAAAA
    CCAGTTGTAC AGATTGCTAT GGAAGCAGGT CAGTTGCCAG CTGCACAAAA TACTTCTATA
    GCAAGACAGC CACAAGCAAC ATCAGGTGAT TCATCACAAC AGTCTTCTAA TCAGCAGCCT
    GAAATGGAGC AAGGATTTCT CCCTAAAGGC TGGGAAGTTC GACATGCACC TAATGGGAGA
    CCATTCTTTA TTGACCACAA TACTAAAACT ACAACATGGG AAGATCCAAG ACTGAAAATT
    CCTGCTCATC TGAGGAGAAA GACTTCCCTA GATCCAGTAG ATCTAGGACC ATTACCACCA
    GGGTGGGAAG AGAGAACACA CACAGACGGA AGAATATTCT TCATAAACCA CAATACCAAG
    AAAACACAAT GGGAGGATCC TCGACTGCAG AACGTGGCAA TAACCGGACC AGCTGTGCCT
    TACTCCAGGG ACTATAAGCG GAAGTACGAG TTCTTCAGAA AGAAACTGAA GAAACAGAGT
    GATATTCCAA ACAGATTTGA AATGAAAATT CATCGCACAA CAATCCTTGA AGATTCTTAC
    AGAAGAATTA TAGCTGTGAA GAGAGCAGAT TTCCTTAAAG CAAGACTTTG GATTGAATTT
    GATGGTGAAA AAGGTTTAGA CTATGGAGGA GTGGCCAGGG AATGGTTCTT CCTTCTCTCT
    AAGGAGATGT TTAATCCTTA CTATGGATTG TTCGAATATT CAGCTACAGA TAACTATACT
    CTGCAGATCA ACCCAAACTC TGGACTGTGC AATGAAGATC ATCTCTCTTA TTTCAAGTTT
    ATAGGTCGAG TAGCAGGAAT GGCTGTTTAC CACGGCAAAC TTTTGGATGC ATTTTTCATT
    CGTCCTTTCT ACAAGATGAT GCTCCAGAAG CCGATAACAC TTCATGATAT GGAGTCTGTG
    GATAGTGAAT ATTATAATTC TCTGAGATGG ATTCTTGAAA ATGATCCAGC GGAACTGGAC
    CTCAGATTTA TAGTTGATGA AGAACTTTTT GGGCAGACTC ATCAGCACGA ACTGAAAAGT
    GGTGGATCAG AAATAGTTGT CACCAACAAA AACAAGAGAG ACTACATTCA TCTTGTAATT
    CAGTGGAGAT TTGTGAGCAG AGTACAGAAA CAAATGGCAG CCTTTAAAGA GGGCTTTTTT
    GAACTAATAC CTCAGGATCT GATTAAAATT TTTGATGAAA ATGAGCTGGA GTTACTGATG
    TGTGGACTGG GAGATGTGGA TGTAGCTGAT TGGAAACTAC ATACAAAATA CAAAAATGGC
    TACAGTGTGA ATCATCAAGT AATACAGTGG TTTTGGAAGG CAGTCTTAAT GATGGACTCT
    GAAAAGAGAA TAAGATTACT ACAGTTTGTT ACTGGCACAT CACGTGTACC TATGAATGGG
    TTTGCTGAGC TTTATGGTGA GTACATAAGA TTGTATTCAC AATTAACTGA GAACATGATA
    CAGCTATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143446
    Clone ID Related Accession (Same CDS sequence) XM_015291877.2
    Accession Version XM_015291877.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3927bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCACGAC GGTTAAGATT GCATTTTGCA ACCCAAAGAA GAAACACAGA TCTGTTATCG 
    GAGACCTCTG AAGATGTTTT GGATAGTAAC AATCAAATGT ACTTCAAAAA GGCACAGAAT
    TCTGAACCTA ATGTCACACA AATGAAGCTG GCTTCTAGGC AAGTTGCTTG TGCACCTGTA
    ATGCAGCAAG TAATTAATCA AGAGGGAAAT GCAACTATTT CTTCACTGCA AAAAAAAAAG
    AGCACGTCAC TGCACATTTC TCTGCAGTCT AGAAGGTATA ATGGATGTTT TCGGTCTGGT
    GATGCTGCAG CTACTGGCGA AGATGCTTCA TTCATCAGTA TTTGTAATGA AGGGAATTGT
    GGCATTAGAA CTGTGGTTGA TCATCAAGCT AATGATAGCC ATCTGAGCAA TACTGCTGTG
    TGCATCGGCA GTCTTAACAG TATTGCAGCC AGTGACAATG GGTCCTTCAG CAGTGCTGGC
    AGTGACTATG GATCATGTGC AAGTACCACG AGTGATGGAG GTTCGTATAG TAACAGCGTC
    ATCAGTGACA GCGGCAGCGG CACTCTCTCG ACAAGCGATG GTTCTTTCTG TTGTAGTGTT
    GTACACAGTG ATTCTTCCTA TGGAAACACT GCAAACAAAT ACAGTCCGTA CAGAAACAAT
    TCATCTCAGG AAGAAATAGA AGTGTGTAGA ATTAATGCTG CTTTTGACCA AGAAGCTTTG
    TCAGTGAGGA AGAAAACTAA AGTTCCGCTG CGGCGGTGTT CATCGTTGGT AATTTTCCCC
    AGAAGTCCTT CCAGTACTCC TCCAGCATCT CCAGTAACCT CAGGTCAGAT GCCACATAGA
    GGTGCCTATC AAACATCACA TAAACTGATT ATTTCTCCCA GTGAGCTGGC GCATAAGGAA
    GATCAAATTG TTTCCAAGGG ATTTCTTTCA ACAGCAGTCA ATGGACTTCG ACTGTCTAAA
    ACAGGTTGTG CATCTGGTGA AGCTAGAGAC ATAAGACCGC TCTACTTGCA TACATCATTG
    CAGGACAAAA GTCAAATTTT GAACAGTTCT GAGCAGGCAG CTCATGAGAG CCTCTCATGT
    ATTTCAGTTC ATCTGACTCC AAGAGCATTT GCATCAAGCA GTGAAATGGT TAATGGCTGT
    TGCAGTCAAG AGTTAAATCT GAACTCCAGT GCAAAATACC CTCCTTTAAA ACTGACACGC
    AGCACATCTG CATGTTTACC CTCAAAAATA GATTCAGAAT ACCAGATGAA TATAAACGAA
    CTGGAAAGAC CGAATGCACA GATGAGCAGA GCCCACCATG TTTTGCAAAG AAGTGTTTCA
    TTGGAAGGAT CGCGTCAGAA AGTTTCTTGC TGTTTATCCA GTCTTAAATA CAAGTGTCAT
    AATGCAAGGA CGTCTCAGTT GCACATACAG TTCAAACCTG GGAACGAAGG AAAAACAAGT
    GGTGTTAAGA AAAGATATGG AACCTCTAAA AGGGAAATAT CTAACACTTC TTTGTGGAGG
    CCCCATAAGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGA TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTTCAAATG GACCACAGTT GTTTACAGTT
    GAACAGTGGG GTACCCCTGA AAAACTGCCG AGAGCTCATA CCTGCTTTAA TCGCTTGGAT
    TTGCCCCCGT ATGATTCATT TGAAGATTTA TGGGATAAAC TTCTTCTAGC GATTGAAAAT
    ACTCAGGGCT TTGATGGGGT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147363.2
    CDS657..4583
    Translation

    Target ORF information:

    RefSeq Version XM_015291877.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291877.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCACGAC GGTTAAGATT GCATTTTGCA ACCCAAAGAA GAAACACAGA TCTGTTATCG 
    GAGACCTCTG AAGATGTTTT GGATAGTAAC AATCAAATGT ACTTCAAAAA GGCACAGAAT
    TCTGAACCTA ATGTCACACA AATGAAGCTG GCTTCTAGGC AAGTTGCTTG TGCACCTGTA
    ATGCAGCAAG TAATTAATCA AGAGGGAAAT GCAACTATTT CTTCACTGCA AAAAAAAAAG
    AGCACGTCAC TGCACATTTC TCTGCAGTCT AGAAGGTATA ATGGATGTTT TCGGTCTGGT
    GATGCTGCAG CTACTGGCGA AGATGCTTCA TTCATCAGTA TTTGTAATGA AGGGAATTGT
    GGCATTAGAA CTGTGGTTGA TCATCAAGCT AATGATAGCC ATCTGAGCAA TACTGCTGTG
    TGCATCGGCA GTCTTAACAG TATTGCAGCC AGTGACAATG GGTCCTTCAG CAGTGCTGGC
    AGTGACTATG GATCATGTGC AAGTACCACG AGTGATGGAG GTTCGTATAG TAACAGCGTC
    ATCAGTGACA GCGGCAGCGG CACTCTCTCG ACAAGCGATG GTTCTTTCTG TTGTAGTGTT
    GTACACAGTG ATTCTTCCTA TGGAAACACT GCAAACAAAT ACAGTCCGTA CAGAAACAAT
    TCATCTCAGG AAGAAATAGA AGTGTGTAGA ATTAATGCTG CTTTTGACCA AGAAGCTTTG
    TCAGTGAGGA AGAAAACTAA AGTTCCGCTG CGGCGGTGTT CATCGTTGGT AATTTTCCCC
    AGAAGTCCTT CCAGTACTCC TCCAGCATCT CCAGTAACCT CAGGTCAGAT GCCACATAGA
    GGTGCCTATC AAACATCACA TAAACTGATT ATTTCTCCCA GTGAGCTGGC GCATAAGGAA
    GATCAAATTG TTTCCAAGGG ATTTCTTTCA ACAGCAGTCA ATGGACTTCG ACTGTCTAAA
    ACAGGTTGTG CATCTGGTGA AGCTAGAGAC ATAAGACCGC TCTACTTGCA TACATCATTG
    CAGGACAAAA GTCAAATTTT GAACAGTTCT GAGCAGGCAG CTCATGAGAG CCTCTCATGT
    ATTTCAGTTC ATCTGACTCC AAGAGCATTT GCATCAAGCA GTGAAATGGT TAATGGCTGT
    TGCAGTCAAG AGTTAAATCT GAACTCCAGT GCAAAATACC CTCCTTTAAA ACTGACACGC
    AGCACATCTG CATGTTTACC CTCAAAAATA GATTCAGAAT ACCAGATGAA TATAAACGAA
    CTGGAAAGAC CGAATGCACA GATGAGCAGA GCCCACCATG TTTTGCAAAG AAGTGTTTCA
    TTGGAAGGAT CGCGTCAGAA AGTTTCTTGC TGTTTATCCA GTCTTAAATA CAAGTGTCAT
    AATGCAAGGA CGTCTCAGTT GCACATACAG TTCAAACCTG GGAACGAAGG AAAAACAAGT
    GGTGTTAAGA AAAGATATGG AACCTCTAAA AGGGAAATAT CTAACACTTC TTTGTGGAGG
    CCCCATAAGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGA TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTTCAAATG GACCACAGTT GTTTACAGTT
    GAACAGTGGG GTACCCCTGA AAAACTGCCG AGAGCTCATA CCTGCTTTAA TCGCTTGGAT
    TTGCCCCCGT ATGATTCATT TGAAGATTTA TGGGATAAAC TTCTTCTAGC GATTGAAAAT
    ACTCAGGGCT TTGATGGGGT TGATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa40463
    Clone ID Related Accession (Same CDS sequence) XM_015291877.1
    Accession Version XM_015291877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCACGAC GGTTAAGATT GCATTTTGCA ACCCAAAGAA GAAACACAGA TCTGTTATCG 
    GAGACCTCTG AAGATGTTTT GGATAGTAAC AATCAAATGT ACTTCAAAAA GGCACAGAAT
    TCTGAGCCTA ATGTCACACA AATGAAGCTG GCTTCTAGGC AAGTTGCTTG TGCACCTGTA
    ATGCAGCAAG TAATTAATCA AGAGGGAAAT GCAACTATTT CTTCACTGCA AAAAAAAAAG
    AGCACGTCAC TGCACATTTC TCTGCAGTCT AGAAGGTATA ATGGATGTTT TCGGTCTGGT
    GATGCTGCAG CTACTGGCGA AGATGCTTCA TTCATCAGTA TTTGTAATGA AGGGAATTGT
    GGCATTAGAA CTGTGGTTGA TCATCAAGCT AATGATAGCC ATCTGAGCAA TACTGCTGTG
    TGCATCGGCA GTCTTAACAG TATTGCAGCC AGTGACAATG GGTCCTTCAG CAGTGCTGGC
    AGTGACTATG GATCATGTGC AAGTACCACG AGTGATGGAG GTTCGTATAG TAACAGCGTC
    ATCAGTGACA GCGGCAGCGG CACTCTCTCG ACAAGCGATG GTTCTTTCTG TTGTAGTGTT
    GTACACAGTG ATTCTTCATA TGGAAACACT GCAAACAAAT ACAGTCCGTA CAGAAACAAT
    TCATCTCAGG AAGAAATAGA AGTGTGTAGA ATTAATGCTG CTTTTGACCA AGAAGCTTTG
    TCAGTGAGGA AGAAAACTAA AGTTCCGCTG CGGCGGTGTT CATCGTTGGT AATTTTCCCC
    AGAAGTCCTT CCAGTACTCC TCCAGCATCT CCAGTAACCT CAGGTCAGAT GCCACATAGA
    GGTGCCTATC AAACATCACA TAAACTGATT ATTTCTCCCA GTGAGCTGGC GCATAAGGAA
    GATCAAATTG TTTCCAAGGG ATTTCTTTCA ACAGCAGTCA ATGGACTTCG ACTGTCTAAA
    ACAGGTTGTG CATCTGGTGA AGCTAGAGAC ATAAGACCGC TCTACTTGCA TACATCATTG
    CAGGACAAAA GTCAAATTTT GAACAGTTCT GAGCAGGCAG CTCATGAGAG CCTCTCATGT
    ATTTCAGTTC ATCTGACTCC AAGAGCATTT GCATCAAGCA GTGAAATGGT TAATGGCTGT
    TGCAGTCAAG AGTTAAATCT GAACTCCAGT GCAAAATACC CTCCTTTAAA ACTGACACGC
    AGCACATCTG CATGTTTACC CTCAAAAATA GATTCAGAAT ACCAGATGAA TATAAACGAA
    CTGGAAAGAC CGAATGCACA GATGAGCAGA GCCCACCATG TTTTGCAAAG AAGTGTTTCA
    TTGGAAGGAT CGCGTCAGAA AGTTTCTTGC TGTTTATCCA GTCTTAAATA CAAGTGTCAT
    AATGCAAGGA CGTCTCAGTT GCACATACAG TTCAAACCTG GGAACGAAGG AAAAACAAGT
    GGTGTTAAGA AAAGATATGG AACCTCTAAA AGGGAAATAT CTAACACTTC TTTGTGGAGG
    CCCCATAAGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGG TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTGAGTACA TAAGATTGTA TTCACAATTA
    ACTGAGAACA TGATACAGCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147363.1
    CDS454..4260
    Translation

    Target ORF information:

    RefSeq Version XM_015291877.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCACGAC GGTTAAGATT GCATTTTGCA ACCCAAAGAA GAAACACAGA TCTGTTATCG 
    GAGACCTCTG AAGATGTTTT GGATAGTAAC AATCAAATGT ACTTCAAAAA GGCACAGAAT
    TCTGAGCCTA ATGTCACACA AATGAAGCTG GCTTCTAGGC AAGTTGCTTG TGCACCTGTA
    ATGCAGCAAG TAATTAATCA AGAGGGAAAT GCAACTATTT CTTCACTGCA AAAAAAAAAG
    AGCACGTCAC TGCACATTTC TCTGCAGTCT AGAAGGTATA ATGGATGTTT TCGGTCTGGT
    GATGCTGCAG CTACTGGCGA AGATGCTTCA TTCATCAGTA TTTGTAATGA AGGGAATTGT
    GGCATTAGAA CTGTGGTTGA TCATCAAGCT AATGATAGCC ATCTGAGCAA TACTGCTGTG
    TGCATCGGCA GTCTTAACAG TATTGCAGCC AGTGACAATG GGTCCTTCAG CAGTGCTGGC
    AGTGACTATG GATCATGTGC AAGTACCACG AGTGATGGAG GTTCGTATAG TAACAGCGTC
    ATCAGTGACA GCGGCAGCGG CACTCTCTCG ACAAGCGATG GTTCTTTCTG TTGTAGTGTT
    GTACACAGTG ATTCTTCATA TGGAAACACT GCAAACAAAT ACAGTCCGTA CAGAAACAAT
    TCATCTCAGG AAGAAATAGA AGTGTGTAGA ATTAATGCTG CTTTTGACCA AGAAGCTTTG
    TCAGTGAGGA AGAAAACTAA AGTTCCGCTG CGGCGGTGTT CATCGTTGGT AATTTTCCCC
    AGAAGTCCTT CCAGTACTCC TCCAGCATCT CCAGTAACCT CAGGTCAGAT GCCACATAGA
    GGTGCCTATC AAACATCACA TAAACTGATT ATTTCTCCCA GTGAGCTGGC GCATAAGGAA
    GATCAAATTG TTTCCAAGGG ATTTCTTTCA ACAGCAGTCA ATGGACTTCG ACTGTCTAAA
    ACAGGTTGTG CATCTGGTGA AGCTAGAGAC ATAAGACCGC TCTACTTGCA TACATCATTG
    CAGGACAAAA GTCAAATTTT GAACAGTTCT GAGCAGGCAG CTCATGAGAG CCTCTCATGT
    ATTTCAGTTC ATCTGACTCC AAGAGCATTT GCATCAAGCA GTGAAATGGT TAATGGCTGT
    TGCAGTCAAG AGTTAAATCT GAACTCCAGT GCAAAATACC CTCCTTTAAA ACTGACACGC
    AGCACATCTG CATGTTTACC CTCAAAAATA GATTCAGAAT ACCAGATGAA TATAAACGAA
    CTGGAAAGAC CGAATGCACA GATGAGCAGA GCCCACCATG TTTTGCAAAG AAGTGTTTCA
    TTGGAAGGAT CGCGTCAGAA AGTTTCTTGC TGTTTATCCA GTCTTAAATA CAAGTGTCAT
    AATGCAAGGA CGTCTCAGTT GCACATACAG TTCAAACCTG GGAACGAAGG AAAAACAAGT
    GGTGTTAAGA AAAGATATGG AACCTCTAAA AGGGAAATAT CTAACACTTC TTTGTGGAGG
    CCCCATAAGA CTCGAGATGA TTTTCTTGGG CAAGTGGATA TTCCCCTTTA TCAGTTACCG
    ACAGAACATC CAAGTATGGA GAGACCATAT ACCTTTAGGG ATTTTGTTCT TCATCCAAGA
    AGCCATAAAT CAAGAGTTAA AGGTCACCTT AGATTAAAGA TGACTTACTT ACCTAAAAGC
    AATGGATCTG AAGAAGAAAC CACAGAACAG GCCGAGGAAT TGGAGCCTGG GTGGATTTTA
    TTGGACCAAC CGGATGCTTC TAACCAGCCA CAACAGCAGC AGCAGGAATC TCCTCCACTG
    CCTTCAGGCT GGGAAGAAAG ACAGGACATC CTTGGCAGGA CCTATTATGT CAATCATGAG
    TTCAGAAGAA CACAGTGGAA AAGACCAACT GCACAGGACA ATGTAGCTGT AGCAGATATT
    GGTAACATGC AGTTGGAGGC CCAGCATGCG TTCACTCATC GGCGGCAGAT ATCAGAGGAT
    ACAGAAAACC TGGACAGCAG GGATTCTCCT GAGAGCTGGG AAATTATAAC TGAAGATGAG
    GCAACATTGT ATAGCAATGA GAACCCTCAA ATACCTCTAC CACAGAACAG TTCTGATATA
    CAGACTCATC TTGCAGAAGA GCTGAATACC AGACTTACTA CCTCTGGAAG TTCAGCTACT
    GGCCAGTCAG CAGCCTACAC TAACCATTCC AGCAGAAGAG GTAGTCTGCA GACATACAGA
    GTTGAAGAGC AGCCTGCACA TCCAGTGTTA CTGCCTACTT CATCTGGATT ACCGCCAGGC
    TGGGAAGAAA GACAAGATGA AAAAGGGAGG TCATATTACA TAGATCACAA TTCCAGAACT
    ACGACTTGGA TAAAACCAGT TGTACAGATT GCTATGGAAG CAGGTCAGTT GCCAGCTGCA
    CAAAATACTT CTATAGCAAG ACAGCCACAA GCAACATCAG GTGATTCATC ACAACAGTCT
    TCTAATCAGC AGCCTGAAAT GGAGCAAGGA TTTCTCCCTA AAGGCTGGGA AGTTCGACAT
    GCACCTAATG GGAGACCATT CTTTATTGAC CACAATACTA AAACTACAAC ATGGGAAGAT
    CCAAGACTGA AAATTCCTGC TCATCTGAGG AGAAAGACTT CCCTAGATCC AGTAGATCTA
    GGACCATTAC CACCAGGGTG GGAAGAGAGA ACACACACAG ACGGAAGAAT ATTCTTCATA
    AACCACAATA CCAAGAAAAC ACAATGGGAG GATCCTCGAC TGCAGAACGT GGCAATAACC
    GGACCAGCTG TGCCTTACTC CAGGGACTAT AAGCGGAAGT ACGAGTTCTT CAGAAAGAAA
    CTGAAGAAAC AGAGTGATAT TCCAAACAGA TTTGAAATGA AAATTCATCG CACAACAATC
    CTTGAAGATT CTTACAGAAG AATTATAGCT GTGAAGAGAG CAGATTTCCT TAAAGCAAGA
    CTTTGGATTG AATTTGATGG TGAAAAAGGT TTAGACTATG GAGGAGTGGC CAGGGAATGG
    TTCTTCCTTC TCTCTAAGGA GATGTTTAAT CCTTACTATG GATTGTTCGA ATATTCAGCT
    ACAGATAACT ATACTCTGCA GATCAACCCA AACTCTGGAC TGTGCAATGA AGATCATCTC
    TCTTATTTCA AGTTTATAGG TCGAGTAGCA GGAATGGCTG TTTACCACGG CAAACTTTTG
    GATGCATTTT TCATTCGTCC TTTCTACAAG ATGATGCTCC AGAAGCCGAT AACACTTCAT
    GATATGGAGT CTGTGGATAG TGAATATTAT AATTCTCTGA GATGGATTCT TGAAAATGAT
    CCAGCGGAAC TGGACCTCAG ATTTATAGTT GATGAAGAAC TTTTTGGGCA GACTCATCAG
    CACGAACTGA AAAGTGGTGG ATCAGAAATA GTTGTCACCA ACAAAAACAA GAGAGACTAC
    ATTCATCTTG TAATTCAGTG GAGATTTGTG AGCAGAGTAC AGAAACAAAT GGCAGCCTTT
    AAAGAGGGCT TTTTTGAACT AATACCTCAG GATCTGATTA AAATTTTTGA TGAAAATGAG
    CTGGAGTTAC TGATGTGTGG ACTGGGAGAT GTGGATGTAG CTGATTGGAA ACTACATACA
    AAATACAAAA ATGGCTACAG TGTGAATCAT CAAGTAATAC AGTGGTTTTG GAAGGCAGTC
    TTAATGATGG ACTCTGAAAA GAGAATAAGA TTACTACAGT TTGTTACTGG CACATCACGT
    GTACCTATGA ATGGGTTTGC TGAGCTTTAT GGTGAGTACA TAAGATTGTA TTCACAATTA
    ACTGAGAACA TGATACAGCT ATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.