ORF » Species Summary » Bos taurus » NEDD4 cDNA ORF clone

NEDD4 cDNA ORF clone, Bos taurus(cattle)

The following NEDD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEDD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06546 XM_003582720.4
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa06546 XM_003586572.5
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46940 XM_010809317.3
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46940 XM_010812047.2
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46938 XM_010809316.3
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46938 XM_010812037.2
Latest version!		 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa46940 XM_010809317.2 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa06546 XM_003586572.4 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46938 XM_010809316.2 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NEDD4 ( XM_003582720.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003582768.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa06546
    Clone ID Related Accession (Same CDS sequence) XM_003582720.4 , XM_003586572.5 , XM_003586572.4
    Accession Version XM_003582720.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582768.1
    CDS91..2796
    Translation

    Target ORF information:

    RefSeq Version XM_003582720.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003582720.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_003586572.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586620.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa06546
    Clone ID Related Accession (Same CDS sequence) XM_003582720.4 , XM_003586572.5 , XM_003586572.4
    Accession Version XM_003586572.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586572.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586620.1
    CDS109..2814
    Translation

    Target ORF information:

    RefSeq Version XM_003586572.5
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003586572.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_003586572.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586620.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa06546
    Clone ID Related Accession (Same CDS sequence) XM_003582720.4 , XM_003586572.5 , XM_003586572.4
    Accession Version XM_003586572.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104281.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586572.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586620.1
    CDS91..2796
    Translation

    Target ORF information:

    RefSeq Version XM_003586572.4
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003586572.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACTT GCGCGGTGGA GGTGTTCGGG CTCCCGGAGG ACGAGGAAAA TTCCCGAATT 
    GTGAGGGTAA AAGTTATAGC TGGAATAGGC CTTGCCAAAA AGGATATCTT GGGAGCTAGT 
    GATCCTTATG TGCGAGTGAC GTTATATGAC CCAATGAATG GAATTTTTAC AAGTGTGCAA 
    ACAAAAACCA TTAAAAAGAC TTTGAATCCA AAATGGAATG AAGAAATATT ATTCAGAGTT 
    CATCCTCAGC AACACCGGCT TCTATTTGAA GTGTTTGATG AAAATCGATT GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010809317.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807619.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa46940
    Clone ID Related Accession (Same CDS sequence) XM_010809317.3 , XM_010809317.2 , XM_010812047.2
    Accession Version XM_010809317.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807619.1
    CDS180..2732
    Translation

    Target ORF information:

    RefSeq Version XM_010809317.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010809317.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010809317.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807619.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa46940
    Clone ID Related Accession (Same CDS sequence) XM_010809317.3 , XM_010809317.2 , XM_010812047.2
    Accession Version XM_010809317.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104281.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807619.1
    CDS130..2682
    Translation

    Target ORF information:

    RefSeq Version XM_010809317.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010809317.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010812047.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810349.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa46940
    Clone ID Related Accession (Same CDS sequence) XM_010809317.3 , XM_010809317.2 , XM_010812047.2
    Accession Version XM_010812047.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810349.1
    CDS130..2682
    Translation

    Target ORF information:

    RefSeq Version XM_010812047.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010812047.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAATGGAA TTTTTACAAG TGTGCAAACA AAAACCATTA AAAAGACTTT GAATCCAAAA 
    TGGAATGAAG AAATATTATT CAGAGTTCAT CCTCAGCAAC ACCGGCTTCT ATTTGAAGTG 
    TTTGATGAAA ATCGATTGAC AAGAGATGAT TTCTTAGGTC AAGTGGATGT TCCTCTTTAT 
    CCATTACCGA CAGAAAATCC AAGAATGGAG AGACCATATA CATTTAAGGA TTTTGTTCTT 
    CATCCAAGAA GTCACAAATC AAGAGTTAAA GGTTATCTGA GATTAAAAAT GACTTACTTA 
    CCTAAAACCA GTGGCTCAGA AGACGATAAC ACAGAACAGG CTGAGGAATT AGAGCCTGGC 
    TGGGTAGTTT TGGACCAACC AGATGCTGCT TGCCATTTGC AGCAGCAACA GGAACCTTCT 
    CCTCTACCTC CAGGATGGGA AGAGAGACAG GATATCCTTG GAAGGACCTA CTATGTAAAC 
    CATGAATCTA GAAGAACACA GTGGAAAAGA CCAACCCTTC AGGACAACCT AACAGATGCT 
    GAGAATGGTA ACATTCAACT GCAAGCCCAG CGTGCATTTA CCACCAGGAG GCAGATATCT 
    GAGGAAACAG AAAGTGTTGA CAACCGAGAA TCTTCTGAGA ACTGGGAAAT TATAAGAGAA 
    GATGAAGCCA CCATGTATGG CCATCAAGCC TTCCCTTCAT CTCCACCATC AAATAACTTG 
    GATGTCCAGA CTCATCTTGC AGAAGAATTG AATGCCAGAC TCACTGTTGG TGGAAATTCA 
    GCCACTGGCC AGCCAGTATC CAACTCAAAT CATTCCAGCA GAAGAGGCAG CTTACAAGCC 
    TATACTTTTG AGGAACAGCC TACACTTCCT GTGCTTTTGC CTACTTCATC TGGATTACCA 
    CCAGGTTGGG AAGAAAAACA AGATGAAAGA GGAAGATCAT ACTATGTGGA TCACAATTGC 
    AGAACTACTA CCTGGATAAA GCCCACTGTA CAGGCCACAG TGGAGACCAG TCAGCTGCCG 
    TCAAGCCAGG GTTCTTCTGC AGGCCCTCAG CCACAGGCCT CCGCGAGTGA TGCAGCACAA 
    CAGGTGACCC AACCATCTGA AATGGAGCAA GGATTCCTTC CCAAAGGCTG GGAGGTCCGG 
    CACGCACCAA ACGGGAGGCC TTTCTTCATT GACCACAACA CCAAAACCAC CACCTGGGAA 
    GATCCAAGAC TGAAAATTCC AGCTCATCTG AGAGGAAAGA CATCACTTGA TTCTTCCAGT 
    GATCTGGGGC CTTTACCTCC AGGATGGGAA GAAAGAACTC ACACCGATGG AAGAATCTTT 
    TACATAAATC ACAATAGTAA AAAAACACAA TGGGAAGACC CTCGTTTGCA GCATGTAGCA 
    ATAACTGGAC CAGCAGTGCC CTACTCCAGG GATTACAAAA GAAAGTATGA GTTCTTCCGA 
    AGAAAGTTGA AGAAGCAGAA TGACATTCCA AACAAATTTG AAATGAAACT TCGCCGTGCA 
    ACTGTTCTTG AGGACTCTTA CAGGAGGATT ATGGGGGTGA AGAGAGCAGA CTTCCTCAAG 
    GCTAGACTGT GGATTGAGTT TGATGGTGAA AAGGGACTAG ATTATGGAGG AGTTGCCAGA 
    GAATGGTTCT TTCTGATTTC AAAGGAAATG TTTAACCCTT ATTATGGGTT GTTTGAATAT 
    TCTGCTACGG ATAATTATAC CCTACAGATA AATCCCAATT CTGGACTGTG TAATGAAGAT 
    CACCTCTCTT ACTTCAAGTT TATTGGTCGG GTAGCTGGGA TGGCAGTGTA TCATGGCAAA 
    CTGTTGGATG GTTTTTTCAT CCGTCCATTT TATAAGATGA TGCTGCACAA GCCAATAACA 
    CTTCATGATA TGGAATCAGT GGATAGTGAG TATTACAATT CATTAAGATG GATTCTTGAA 
    AATGATCCAA CAGAATTGGA CCTCAGGTTT GTCATAGATG AAGAACTTTT TGGACAGACA 
    CATCAACATG AGTTGAAAAG TGGTGGATCA GAAATAGTTG TCACCAATAA GAACAAAAAG 
    GAATATATTT ATCTTGTAAT ACAGTGGCGA TTTGTAAACC GAATCCAGAA GCAAATGGCT 
    GCTTTTAAAG AGGGGTTTTT TGAACTGATA CCACAGGATC TCATCAAAAT TTTTGATGAA 
    AATGAACTAG AGCTTCTGAT GTGTGGCTTG GGAGATGTGG ATGTGAATGA CTGGAGAGAA 
    CACACAAAGT ACAAAAATGG CTACAGCATA AATCATCAAG TCATCCAGTG GTTTTGGAAG 
    GCTGTTTTAA TGATGGACTC AGAAAAAAGA ATACGATTAC TTCAGTTTGT CACTGGCACA 
    TCCCGTGTGC CTATGAATGG ATTTGCTGAA CTGTATGGCT CAAACGGACC ACAGTCATTT 
    ACAGTTGAGC AGTGGGGCAC ACCTGAGAAG CTGCCAAGAG CTCATACCTG CTTTAATCGC 
    CTGGACTTGC CACCCTATGA GTCATTTGAA GAATTATGGG ATAAACTTCA GATGGCCATT 
    GAGAACACTC AGGGTTTTGA TGGCGTTGAT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010809316.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807618.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa46938
    Clone ID Related Accession (Same CDS sequence) XM_010809316.2 , XM_010812037.2 , XM_010809316.3
    Accession Version XM_010809316.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104281.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807618.1
    CDS571..4536
    Translation

    Target ORF information:

    RefSeq Version XM_010809316.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010809316.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010812037.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810339.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa46938
    Clone ID Related Accession (Same CDS sequence) XM_010809316.2 , XM_010812037.2 , XM_010809316.3
    Accession Version XM_010812037.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645880.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010812037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810339.1
    CDS571..4536
    Translation

    Target ORF information:

    RefSeq Version XM_010812037.2
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010812037.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NEDD4 ( XM_010809316.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010807618.1 Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa46938
    Clone ID Related Accession (Same CDS sequence) XM_010809316.2 , XM_010812037.2 , XM_010809316.3
    Accession Version XM_010809316.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase NEDD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807618.1
    CDS556..4521
    Translation

    Target ORF information:

    RefSeq Version XM_010809316.3
    Organism Bos taurus(cattle)
    Definition Bos taurus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010809316.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCACAAA GCTTACGATT GCACTTTGCA GCCAGAAGAA GCAATACTTA TCCTTTGTCA 
    GAAACCTCCG GAGATGATTT GGATAGCAGC ATTCACATGT GCTTCACGAG ACCAACACGG 
    ATTTCAACGG CGAACGTGGT TCAAATGAAG CTGACTCCCA AACAGACTGC ACTAGCTCCG 
    TTAATAAAGG AAAACCTTAA GTCTCGGGAA AGATCATCTT TTCCTTCACC TGAAAATGTT 
    CATAAAAAGA GCAGCTGTCT GCAGATATCA TTACAACCAA CAAGGTACAG TGGATGTCTT 
    CAGTCTGGCA ATGTCTTAGC TGATGGTGAT GATGCTTCGT TTACTTGTGT CTTAAAGGAT 
    GGCAGTGCTG TGGTTGATAA TGAACTGAAT GCCATGGATG ATGGTAACCT CTTAAGCAGT 
    CCTGCCATTT GCAGTGGTAG TCTTAGTAAC TTTGCAGCCA GTGAAAATGG GTCTTACAGC 
    AGCAATGGTA GTGATTATGG GTCCTGCACA AGTATCACCA GTGGAGGTTC ATACACCAAC 
    AGTAACATCA GTGACAGCAG TGGTTATACT TTTCCACCAA CTGATGATAC CTTTTTTGGT 
    GGAAATTTAT CTTCTGACAG CGCCTCCAAT AGAAGTGTGC CAAACAGGAA TACTACTCCA 
    TGTGAAATTT TTTCAAGAAG TACAAGTACA GTTCCCTTTG TCCAGGATGA CTTGGATCAT 
    GGACTAGAGA TTATGAAATT GCCAGTGAGC AGGAACTCGA AAATTCCACT AAAACGTTGT 
    TCATCCTTAG TCATTTTTCC TAGGAGTCCT TCAAATACCC CACCAACTTC TCCAACAAGT 
    ACAGGTACTC TTTCAAGCAA AGGATCCTAT CAGACCTCAC ACCAGTTTAT TATTTCTCCT 
    AGTGAAATTG CCCACAATGA AGATGGCACT AGTGCTAAGG GATTTCTTTC AACAGCTGTC 
    AATGGACTTC GGTTATCCAA GACAGTCTGT ACTCCCGGAG AAGTAAGAGA CATACGGCCT 
    CTTCACAGGA AGGGCTCGTT ACAAAAGAAA ATCATTATTG CAAATAATAG TCCAAATCAG 
    ACTGTCTGTG AAAAGCCATC TGAAGGATAT TCTTGTGTTT CACTGCATTT TACCCAACAG 
    AAAGCAACTG CATTAGATTG TGAAGCAGCA AATGGTGATT GTAAACCAGA GATGTCAGAA 
    ACTGAGCTTA ATTCTGATCC AGAGTTTATT AAGCTTATGC ATAGGACATC TGCATGTTTA 
    CCATCCTCCC AAAATGTAGA TTATCAAATA AATATCAATG GACAGTTGGA AAGACCAAAT 
    TTACAGATAA ACAAAAACCA TGCTCTTTTA CAGAGAAGTA TTTCATTGGG GGGAACTTAT 
    CCAAATGTTT CCTGTTTATC TAGCATTAAG CACAATTGTT CTAAGGGGGG ACCATCTCAA 
    TTACTCATAA AGTTTGCATC TGGAAATGAA GGTAAAGTTG ATAATTTAAC CAGAGACAGC 
    AACAGAGATT TCACAAAAGA ACTACCTAAT TCTTTAAAGC ATTCTTGTAA GACAAGAGAT 
    GATTTCTTAG GTCAAGTGGA TGTTCCTCTT TATCCATTAC CGACAGAAAA TCCAAGAATG 
    GAGAGACCAT ATACATTTAA GGATTTTGTT CTTCATCCAA GAAGTCACAA ATCAAGAGTT 
    AAAGGTTATC TGAGATTAAA AATGACTTAC TTACCTAAAA CCAGTGGCTC AGAAGACGAT 
    AACACAGAAC AGGCTGAGGA ATTAGAGCCT GGCTGGGTAG TTTTGGACCA ACCAGATGCT 
    GCTTGCCATT TGCAGCAGCA ACAGGAACCT TCTCCTCTAC CTCCAGGATG GGAAGAGAGA 
    CAGGATATCC TTGGAAGGAC CTACTATGTA AACCATGAAT CTAGAAGAAC ACAGTGGAAA 
    AGACCAACCC TTCAGGACAA CCTAACAGAT GCTGAGAATG GTAACATTCA ACTGCAAGCC 
    CAGCGTGCAT TTACCACCAG GAGGCAGATA TCTGAGGAAA CAGAAAGTGT TGACAACCGA 
    GAATCTTCTG AGAACTGGGA AATTATAAGA GAAGATGAAG CCACCATGTA TGGCCATCAA 
    GCCTTCCCTT CATCTCCACC ATCAAATAAC TTGGATGTCC AGACTCATCT TGCAGAAGAA 
    TTGAATGCCA GACTCACTGT TGGTGGAAAT TCAGCCACTG GCCAGCCAGT ATCCAACTCA 
    AATCATTCCA GCAGAAGAGG CAGCTTACAA GCCTATACTT TTGAGGAACA GCCTACACTT 
    CCTGTGCTTT TGCCTACTTC ATCTGGATTA CCACCAGGTT GGGAAGAAAA ACAAGATGAA 
    AGAGGAAGAT CATACTATGT GGATCACAAT TGCAGAACTA CTACCTGGAT AAAGCCCACT 
    GTACAGGCCA CAGTGGAGAC CAGTCAGCTG CCGTCAAGCC AGGGTTCTTC TGCAGGCCCT 
    CAGCCACAGG CCTCCGCGAG TGATGCAGCA CAACAGGTGA CCCAACCATC TGAAATGGAG 
    CAAGGATTCC TTCCCAAAGG CTGGGAGGTC CGGCACGCAC CAAACGGGAG GCCTTTCTTC 
    ATTGACCACA ACACCAAAAC CACCACCTGG GAAGATCCAA GACTGAAAAT TCCAGCTCAT 
    CTGAGAGGAA AGACATCACT TGATTCTTCC AGTGATCTGG GGCCTTTACC TCCAGGATGG 
    GAAGAAAGAA CTCACACCGA TGGAAGAATC TTTTACATAA ATCACAATAG TAAAAAAACA 
    CAATGGGAAG ACCCTCGTTT GCAGCATGTA GCAATAACTG GACCAGCAGT GCCCTACTCC 
    AGGGATTACA AAAGAAAGTA TGAGTTCTTC CGAAGAAAGT TGAAGAAGCA GAATGACATT 
    CCAAACAAAT TTGAAATGAA ACTTCGCCGT GCAACTGTTC TTGAGGACTC TTACAGGAGG 
    ATTATGGGGG TGAAGAGAGC AGACTTCCTC AAGGCTAGAC TGTGGATTGA GTTTGATGGT 
    GAAAAGGGAC TAGATTATGG AGGAGTTGCC AGAGAATGGT TCTTTCTGAT TTCAAAGGAA 
    ATGTTTAACC CTTATTATGG GTTGTTTGAA TATTCTGCTA CGGATAATTA TACCCTACAG 
    ATAAATCCCA ATTCTGGACT GTGTAATGAA GATCACCTCT CTTACTTCAA GTTTATTGGT 
    CGGGTAGCTG GGATGGCAGT GTATCATGGC AAACTGTTGG ATGGTTTTTT CATCCGTCCA 
    TTTTATAAGA TGATGCTGCA CAAGCCAATA ACACTTCATG ATATGGAATC AGTGGATAGT 
    GAGTATTACA ATTCATTAAG ATGGATTCTT GAAAATGATC CAACAGAATT GGACCTCAGG 
    TTTGTCATAG ATGAAGAACT TTTTGGACAG ACACATCAAC ATGAGTTGAA AAGTGGTGGA 
    TCAGAAATAG TTGTCACCAA TAAGAACAAA AAGGAATATA TTTATCTTGT AATACAGTGG 
    CGATTTGTAA ACCGAATCCA GAAGCAAATG GCTGCTTTTA AAGAGGGGTT TTTTGAACTG 
    ATACCACAGG ATCTCATCAA AATTTTTGAT GAAAATGAAC TAGAGCTTCT GATGTGTGGC 
    TTGGGAGATG TGGATGTGAA TGACTGGAGA GAACACACAA AGTACAAAAA TGGCTACAGC 
    ATAAATCATC AAGTCATCCA GTGGTTTTGG AAGGCTGTTT TAATGATGGA CTCAGAAAAA 
    AGAATACGAT TACTTCAGTT TGTCACTGGC ACATCCCGTG TGCCTATGAA TGGATTTGCT 
    GAACTGTATG GCTCAAACGG ACCACAGTCA TTTACAGTTG AGCAGTGGGG CACACCTGAG 
    AAGCTGCCAA GAGCTCATAC CTGCTTTAAT CGCCTGGACT TGCCACCCTA TGAGTCATTT 
    GAAGAATTAT GGGATAAACT TCAGATGGCC ATTGAGAACA CTCAGGGTTT TGATGGCGTT 
    GATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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