ttc3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttc3
Entrez Gene ID	100492684
Full Name	tetratricopeptide repeat domain 3
General protein information	Preferred Names tetratricopeptide repeat domain 3 Names E3 ubiquitin-protein ligase TTC3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031896550.1	XP_031752410.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_004912114.3	XP_004912171.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_018091393.1	XP_017946882.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_004912114.4	XP_004912171.2	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_018091392.1	XP_017946881.1	E3 ubiquitin-protein ligase TTC3 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ttc3	NP_001101785.1
Bos taurus (cattle)	TTC3	NP_001103237.1
Xenopus tropicalis (tropical clawed frog)	ttc3	XP_002941578.2
	TTC3	XP_001098049.2
Gallus gallus (chicken)	TTC3	XP_416727.4
Danio rerio (zebrafish)	LOC100329450	XP_005172636.1
Pan troglodytes (chimpanzee)	TTC3	XP_001169932.1
Canis lupus familiaris (dog)	TTC3	XP_849567.2
Mus musculus (house mouse)	Ttc3	NP_033467.2
Homo sapiens (human)	TTC3	NP_001001894.1

Gene Ontology

The following ttc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42624	XM_018091392.1 Latest version!	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa42625	XM_018091393.1 Latest version!	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa51934	XM_004912114.4 Latest version!	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa51935	XM_031896550.1 Latest version!	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa06400	XM_004912114.3 Latest version!	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttc3 ( XM_018091392.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946881.1 Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa42624
Clone ID Related Accession (Same CDS sequence)	XM_018091392.1
Accession Version	XM_018091392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGAA GAGAAATCCA ATGGCAACTC TCTCCTTGCA GGGTTTTATC 
CGTGTGATAT GTTGCCTGTC ATGTAAAGTG GAGTTCCATG TTTGCTGCTG GAAGAAGCTG 
AAGGCCAACA ACTATTCCGA TAAAGCTGAC AGGGATTTTC TAACTATCTC TTGTTTTACA 
CCTGACTGTA AAGGCAAAAT CTCTAATATA ATTATTTATG ACTCCACTGG ACTTGTAAAA 
CGTGAGTTTG CAGATAAGTC TGTTAAGAAG AAAGAACCAC AAAGGCCAAC AGTTAAGAAT 
AAAGGAAGTA GCAATAAGAA GTTAAAGATT AAGCCTGAGA GTAAAACACA AGCAAAGAAG 
ACCTCACCTG AAGAGAACAG GAGCTTGATA CACAATGAGA AATCACAGAA GCAGCAAGAA 
AATATCTCCT CTAACCAACG GAATAATATA TGTAATAACC GTTATAGTGT AGCACCTCAT 
GATTTAATGA TGACGCTTAT ATTGAATAAG GAGGAGGTTT TTAAAACAGG AGTGGAGAGC 
ACCAAAGCAT TGTGCAACTC ATTAATCTCA TGGAATGTTA TAAGTCAGGA AGAACTTGAT 
GGTTTTTCCT TTGAAACAAC ATGTAGATCG TCTTCTGATG AAATGGCAGT CCTGCTCGCT 
CATCTGTGCA GCTTAAATAA TAGAGTAAAG ACAAGAATAT TCCTCTACCT GCTGGAAGAT 
TATCCTTTAA CTTTAGAACT GCCTGCATTT ATAAAGATGA TTAGAGATAC GGGTCATCGG 
GCAGCAAAGA AGTTCCAAGA GATAAACAGC AGCCTCCTGA AAGAGCTTAA TTTCGAGTTG 
CTAGCCGGTC GGTGGAACGA AACCTACGGA AAACTTTTAA ACTTCTTCAT GTCTATATCT 
GTGGAAACAG CTGAGAAACT AAGTCAAGCA AATATCCAAA TATTCCGCTG CTTCATATGG 
TTTCTAGACG ACAATAGATA TGTCCCCATG CCTGCCGGGC TGGATAAATA TTTAGATAAA 
TATTTTATAG ATATGGATAT ACCATATTCC AAAAATGATG TGCTCGACTC TGTTAATAAA 
GACCCTATAG AAGCCCCTTG TTGCATCAAT ATACGAATGA AGTCAGATGA AGAGCAGCCT 
ACTAAATGTA TCAAGGTTAA GAACAAACAC AAGAAAAAGA AGCAAAATCA GCCCAAGACT 
TTGTATAAGG TTTCTGGTGC AGTAAGTACC AGGCCTCCAG AAGATGACAT TTTTACAGAA 
GAAAATACTC TAAGCCTGTT GGATCCTTAT GAACCCTTTA TAATCCCAGA ATATTTGCGT 
CCTGATGTGG AAGAATTTGA AACCCTTTAT GATCCCACTC CTGGCGGATA CCGGTTACCA 
TGGCAACCTG ATAATGCCAT GCAAGAAACC CTCTATGAAT ATTTTTCTGA GATCCTGGAA 
GAGCATGGGC CTTTGGAACT GGATGATGAC ATTCTGATTG GGACATTTAA GGAGTTCCCA 
GAGGAGGCAC ACCAGCTGGT ACAGGAGGCC GGCAGCCTAA AGGACTTCCT TCTGCAATCA 
ACTAAGTTTG TCATGGTGGG AAAGCTCCTG GGACTGCGCC CTCACAAAGT TATATTGGAA 
GAAGCATCCG GAAAAGCCAA ACTCAACCCA ACCGCTAAAG AATTCCAGCC TTCATCTAAT 
AATCCTTTTG ATTCAATAAT AAGCGAGACT TTGGAAATAA GTCCTGAATA CAATCCACTT 
GAGCCATCCA ATCCCTTTGT TTTACTGCCC AATCAAAGCC CAGTTTTTAA TGGCAATTGT 
ATAGTCCCTT GTCTTCCGCT GTATGGACTG AATCCTTTGC CTGTAGTTTC TGTAGGGCAC 
CCATTTATAC CTGGTCTCGG CATTGGAATG CAGCCTACGA TAACAGTTAA TAGTGCTGTT 
ATGTGTAATT TAGGCGTGCC GCTTCCTAAT AGAGCAAAGG AGCCAGAGCA GTCAAGTCCA 
ATAATGCATA ACACTTTGAC TGGGAATTTG AATCCCAAAA ATGACACTGC ATCAGAGACC 
ACCATGACCC AGACTGTTCC TTTAAGAAGA AATATTCACA CCGGGGTAGT GTCAGTACAG 
GTAGACAGAG ACTTATCTGA GCAAGAAGTA AATACTGAGC CTTATTATCC TTTTGAAGTG 
CAGCAGGGAG ATATCCTACG GATAGAGAAA GAGCATCTCG TTCTACAGGA GCAGCTAAAA 
GAAGCAACAG AGAAGTATGA GAATCTGCAG GGCCGCTACA AGGAGGAAAT CGCTGGGTTG 
CATAAGCAAA TTAGGGAAAC GGCTGACAAT AATAAGATTA TAAAGAAAGA ACTTGAAATG 
TTTAACCACG AGATGGATAA CGACACCAAA AAATGGCAAC TAGAGAAAAA AGAGTATCAT 
GATAAGCTGA GAGTGGTAAA GAGCGGCATT AAGGCGGCAA ATGAAACTGT CGATAAGTAT 
TCAAGGGAGA TTGAAGAAAA GAAACAACAG TATGAAGCAT ACATAGAGAC ATTCGTACAG 
TCTGGTTTTA ATATTGAAAA TGAAAAGGCA AAGTTGGAAA AGCATATTAA AAGCAACGAG 
GATAAGCTCC AGGAAAGCAA GAAGAGAGCT ATGACTGCTG AGGTCCACTT CCTTGAGAAC 
AAAAAACAGA CTGATTTATT AAAGCTGAGG ATTATGGCTG CAAAGGTGGA ACACAGCGCC 
AACAAGTTAA AAGCCAATGG CTCTGCCACA CCTTTTGCAC AAAAAAGCAT AGCGGATTTA 
TCCCCCTCTT ATCTAAAATC TGAAAGTGAG AAAATAAAGT CCCAGTTTGA TGAGAAGATT 
AAATTAGTGG AAAGTGGATA TAAGTTGGCA AATATACAAG CAAACAATCT GGCAGCTCCA 
TTCAATGCAT CTGCTTTTCA AACACCTCTG CTGCCCCCTT CTCCCACCCC CAAGCTCCCT 
ACAACCAAAC CCAGCACCCC GCTGCCAGCT TCCAGTTCTG CACCTCCAGC TTCCCTCAAG 
TCCCCATTAA AGCCGACTCG CGAAACCAAG AAAAGCAAAT CTAAAGATAT TAATAAGGAT 
CCGAAACCAC CACCTCCTGC AATTAATCCA AAGGCCACGG CATTAGCAGG GAAGGGCCCG 
GGAGCACCCG TCATGGAGAA GAAAGGAAAG CCCAACTTAG AGGCACAACA TCAGTCTCCC 
GGAAAGCCGA CTGCGTTTGA TAAGATTATT GAGGAGCTGC ATGAGATTTT TCCTGACTAC 
AAAAGTGCGG AGCTTACCAA CTTCATCAAG GAGCTGCGCA ATAAGACTGG CGGAACACTG 
GCTGGCATGA AGCACGATGA CATTGTTAGC AGGGTGACTG AGTATATACT GGACTCCCAG 
GATAAGGCCC CCCCACAGGC ACCTTGGGTA CAAGCAAGTG GCAATGTATA TCCTTCAGCA 
GTGAAACCCC AGGCCCCATC GCCCCAGCCC AAACAGCCCT GGAGAGTGGT CACTGGTCCC 
TCCAAGAACA AGTGGCAGAA GTCTGATGAT ACAGAGTGCT ATAATGATGA GCCGTGCATT 
ATCTGCCATG ATGAGCTGCA ACAGTATCCG GTGCAGAAGC TGGACTGTGG GCACTGCTTC 
CATAGACACT GCATAAAGAC CTGGCTGAAC ACACAGAGCA CCTGCCCGAC CTGCAGAGAT 
CACGCTCTCC TTCCTGAGGA CTTCCCGGCC CTTTCTGGGC GCATAAGAAC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946881.1
CDS	133..5829
Translation	MGVPGRYCYFPLSRSRIWAGGLPCSFSISAPWDCGWDPSAMDGIGYTDIGNGSSHVIATCFRGHEYPMQRPPVPVGEKVDPVTFWLSHPLDVIHEHCDLIKVYILWPVLKADASPVYKGPWVDHDGSGLCIQELNFVEMLEDLLDVVKKVAHERRFIEGLLKIAKTMEMVQLNYQHLSEAMSWLRSTGELQICKKLEEQDNLSLFPLQILFIDYAHYINSMADQRCSLSFMWKKKHICDCCAVESEGMKQRGNEEFSKSNFCLAADLYSKAIEYSPDNHLLFGNRSLCFLRMGEYMNALSDGKKATVLKHKWPKGHYRFCEALFLMGERERALASNEKAQLLCKDSPEGIRDLIQQHTKIKRKIEEMKDGKHRRSTNKKFIRDKKIAPYSGPMSVLKEKKKIPSSNKEDIPNKESDGATLENKDIISDNVTGQNQKKGKSKGKGGESEKLRVSNAKSDPKENQKDVSCNYSQMDLSTLQDMVKSLVRDGCEALADSRCHNALGYFSKLLDILAASDLQRFYLSNIDYVVLLYGYANALLGTGQCEELTKAEDQLKKIITQFTKERFNCLAFYGIGNVYYRQNRFSDALNQYVKSHTMVKLKMMPGILTWPTTPIVLEESRPEKLQSLLENRIEECKFPPKPDAICRYKQCLSLPKPQIYFTDPDFKKRNPMATLSLQGFIRVICCLSCKVEFHVCCWKKLKANNYSDKADRDFLTISCFTPDCKGKISNIIIYDSTGLVKREFADKSVKKKEPQRPTVKNKGSSNKKLKIKPESKTQAKKTSPEENRSLIHNEKSQKQQENISSNQRNNICNNRYSVAPHDLMMTLILNKEEVFKTGVESTKALCNSLISWNVISQEELDGFSFETTCRSSSDEMAVLLAHLCSLNNRVKTRIFLYLLEDYPLTLELPAFIKMIRDTGHRAAKKFQEINSSLLKELNFELLAGRWNETYGKLLNFFMSISVETAEKLSQANIQIFRCFIWFLDDNRYVPMPAGLDKYLDKYFIDMDIPYSKNDVLDSVNKDPIEAPCCINIRMKSDEEQPTKCIKVKNKHKKKKQNQPKTLYKVSGAVSTRPPEDDIFTEENTLSLLDPYEPFIIPEYLRPDVEEFETLYDPTPGGYRLPWQPDNAMQETLYEYFSEILEEHGPLELDDDILIGTFKEFPEEAHQLVQEAGSLKDFLLQSTKFVMVGKLLGLRPHKVILEEASGKAKLNPTAKEFQPSSNNPFDSIISETLEISPEYNPLEPSNPFVLLPNQSPVFNGNCIVPCLPLYGLNPLPVVSVGHPFIPGLGIGMQPTITVNSAVMCNLGVPLPNRAKEPEQSSPIMHNTLTGNLNPKNDTASETTMTQTVPLRRNIHTGVVSVQVDRDLSEQEVNTEPYYPFEVQQGDILRIEKEHLVLQEQLKEATEKYENLQGRYKEEIAGLHKQIRETADNNKIIKKELEMFNHEMDNDTKKWQLEKKEYHDKLRVVKSGIKAANETVDKYSREIEEKKQQYEAYIETFVQSGFNIENEKAKLEKHIKSNEDKLQESKKRAMTAEVHFLENKKQTDLLKLRIMAAKVEHSANKLKANGSATPFAQKSIADLSPSYLKSESEKIKSQFDEKIKLVESGYKLANIQANNLAAPFNASAFQTPLLPPSPTPKLPTTKPSTPLPASSSAPPASLKSPLKPTRETKKSKSKDINKDPKPPPPAINPKATALAGKGPGAPVMEKKGKPNLEAQHQSPGKPTAFDKIIEELHEIFPDYKSAELTNFIKELRNKTGGTLAGMKHDDIVSRVTEYILDSQDKAPPQAPWVQASGNVYPSAVKPQAPSPQPKQPWRVVTGPSKNKWQKSDDTECYNDEPCIICHDELQQYPVQKLDCGHCFHRHCIKTWLNTQSTCPTCRDHALLPEDFPALSGRIRTT

Target ORF information:

RefSeq Version	XM_018091392.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091392.1

ORF Insert Sequence:

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGAA GAGAAATCCA ATGGCAACTC TCTCCTTGCA GGGTTTTATC 
CGTGTGATAT GTTGCCTGTC ATGTAAAGTG GAGTTCCATG TTTGCTGCTG GAAGAAGCTG 
AAGGCCAACA ACTATTCCGA TAAAGCTGAC AGGGATTTTC TAACTATCTC TTGTTTTACA 
CCTGACTGTA AAGGCAAAAT CTCTAATATA ATTATTTATG ACTCCACTGG ACTTGTAAAA 
CGTGAGTTTG CAGATAAGTC TGTTAAGAAG AAAGAACCAC AAAGGCCAAC AGTTAAGAAT 
AAAGGAAGTA GCAATAAGAA GTTAAAGATT AAGCCTGAGA GTAAAACACA AGCAAAGAAG 
ACCTCACCTG AAGAGAACAG GAGCTTGATA CACAATGAGA AATCACAGAA GCAGCAAGAA 
AATATCTCCT CTAACCAACG GAATAATATA TGTAATAACC GTTATAGTGT AGCACCTCAT 
GATTTAATGA TGACGCTTAT ATTGAATAAG GAGGAGGTTT TTAAAACAGG AGTGGAGAGC 
ACCAAAGCAT TGTGCAACTC ATTAATCTCA TGGAATGTTA TAAGTCAGGA AGAACTTGAT 
GGTTTTTCCT TTGAAACAAC ATGTAGATCG TCTTCTGATG AAATGGCAGT CCTGCTCGCT 
CATCTGTGCA GCTTAAATAA TAGAGTAAAG ACAAGAATAT TCCTCTACCT GCTGGAAGAT 
TATCCTTTAA CTTTAGAACT GCCTGCATTT ATAAAGATGA TTAGAGATAC GGGTCATCGG 
GCAGCAAAGA AGTTCCAAGA GATAAACAGC AGCCTCCTGA AAGAGCTTAA TTTCGAGTTG 
CTAGCCGGTC GGTGGAACGA AACCTACGGA AAACTTTTAA ACTTCTTCAT GTCTATATCT 
GTGGAAACAG CTGAGAAACT AAGTCAAGCA AATATCCAAA TATTCCGCTG CTTCATATGG 
TTTCTAGACG ACAATAGATA TGTCCCCATG CCTGCCGGGC TGGATAAATA TTTAGATAAA 
TATTTTATAG ATATGGATAT ACCATATTCC AAAAATGATG TGCTCGACTC TGTTAATAAA 
GACCCTATAG AAGCCCCTTG TTGCATCAAT ATACGAATGA AGTCAGATGA AGAGCAGCCT 
ACTAAATGTA TCAAGGTTAA GAACAAACAC AAGAAAAAGA AGCAAAATCA GCCCAAGACT 
TTGTATAAGG TTTCTGGTGC AGTAAGTACC AGGCCTCCAG AAGATGACAT TTTTACAGAA 
GAAAATACTC TAAGCCTGTT GGATCCTTAT GAACCCTTTA TAATCCCAGA ATATTTGCGT 
CCTGATGTGG AAGAATTTGA AACCCTTTAT GATCCCACTC CTGGCGGATA CCGGTTACCA 
TGGCAACCTG ATAATGCCAT GCAAGAAACC CTCTATGAAT ATTTTTCTGA GATCCTGGAA 
GAGCATGGGC CTTTGGAACT GGATGATGAC ATTCTGATTG GGACATTTAA GGAGTTCCCA 
GAGGAGGCAC ACCAGCTGGT ACAGGAGGCC GGCAGCCTAA AGGACTTCCT TCTGCAATCA 
ACTAAGTTTG TCATGGTGGG AAAGCTCCTG GGACTGCGCC CTCACAAAGT TATATTGGAA 
GAAGCATCCG GAAAAGCCAA ACTCAACCCA ACCGCTAAAG AATTCCAGCC TTCATCTAAT 
AATCCTTTTG ATTCAATAAT AAGCGAGACT TTGGAAATAA GTCCTGAATA CAATCCACTT 
GAGCCATCCA ATCCCTTTGT TTTACTGCCC AATCAAAGCC CAGTTTTTAA TGGCAATTGT 
ATAGTCCCTT GTCTTCCGCT GTATGGACTG AATCCTTTGC CTGTAGTTTC TGTAGGGCAC 
CCATTTATAC CTGGTCTCGG CATTGGAATG CAGCCTACGA TAACAGTTAA TAGTGCTGTT 
ATGTGTAATT TAGGCGTGCC GCTTCCTAAT AGAGCAAAGG AGCCAGAGCA GTCAAGTCCA 
ATAATGCATA ACACTTTGAC TGGGAATTTG AATCCCAAAA ATGACACTGC ATCAGAGACC 
ACCATGACCC AGACTGTTCC TTTAAGAAGA AATATTCACA CCGGGGTAGT GTCAGTACAG 
GTAGACAGAG ACTTATCTGA GCAAGAAGTA AATACTGAGC CTTATTATCC TTTTGAAGTG 
CAGCAGGGAG ATATCCTACG GATAGAGAAA GAGCATCTCG TTCTACAGGA GCAGCTAAAA 
GAAGCAACAG AGAAGTATGA GAATCTGCAG GGCCGCTACA AGGAGGAAAT CGCTGGGTTG 
CATAAGCAAA TTAGGGAAAC GGCTGACAAT AATAAGATTA TAAAGAAAGA ACTTGAAATG 
TTTAACCACG AGATGGATAA CGACACCAAA AAATGGCAAC TAGAGAAAAA AGAGTATCAT 
GATAAGCTGA GAGTGGTAAA GAGCGGCATT AAGGCGGCAA ATGAAACTGT CGATAAGTAT 
TCAAGGGAGA TTGAAGAAAA GAAACAACAG TATGAAGCAT ACATAGAGAC ATTCGTACAG 
TCTGGTTTTA ATATTGAAAA TGAAAAGGCA AAGTTGGAAA AGCATATTAA AAGCAACGAG 
GATAAGCTCC AGGAAAGCAA GAAGAGAGCT ATGACTGCTG AGGTCCACTT CCTTGAGAAC 
AAAAAACAGA CTGATTTATT AAAGCTGAGG ATTATGGCTG CAAAGGTGGA ACACAGCGCC 
AACAAGTTAA AAGCCAATGG CTCTGCCACA CCTTTTGCAC AAAAAAGCAT AGCGGATTTA 
TCCCCCTCTT ATCTAAAATC TGAAAGTGAG AAAATAAAGT CCCAGTTTGA TGAGAAGATT 
AAATTAGTGG AAAGTGGATA TAAGTTGGCA AATATACAAG CAAACAATCT GGCAGCTCCA 
TTCAATGCAT CTGCTTTTCA AACACCTCTG CTGCCCCCTT CTCCCACCCC CAAGCTCCCT 
ACAACCAAAC CCAGCACCCC GCTGCCAGCT TCCAGTTCTG CACCTCCAGC TTCCCTCAAG 
TCCCCATTAA AGCCGACTCG CGAAACCAAG AAAAGCAAAT CTAAAGATAT TAATAAGGAT 
CCGAAACCAC CACCTCCTGC AATTAATCCA AAGGCCACGG CATTAGCAGG GAAGGGCCCG 
GGAGCACCCG TCATGGAGAA GAAAGGAAAG CCCAACTTAG AGGCACAACA TCAGTCTCCC 
GGAAAGCCGA CTGCGTTTGA TAAGATTATT GAGGAGCTGC ATGAGATTTT TCCTGACTAC 
AAAAGTGCGG AGCTTACCAA CTTCATCAAG GAGCTGCGCA ATAAGACTGG CGGAACACTG 
GCTGGCATGA AGCACGATGA CATTGTTAGC AGGGTGACTG AGTATATACT GGACTCCCAG 
GATAAGGCCC CCCCACAGGC ACCTTGGGTA CAAGCAAGTG GCAATGTATA TCCTTCAGCA 
GTGAAACCCC AGGCCCCATC GCCCCAGCCC AAACAGCCCT GGAGAGTGGT CACTGGTCCC 
TCCAAGAACA AGTGGCAGAA GTCTGATGAT ACAGAGTGCT ATAATGATGA GCCGTGCATT 
ATCTGCCATG ATGAGCTGCA ACAGTATCCG GTGCAGAAGC TGGACTGTGG GCACTGCTTC 
CATAGACACT GCATAAAGAC CTGGCTGAAC ACACAGAGCA CCTGCCCGAC CTGCAGAGAT 
CACGCTCTCC TTCCTGAGGA CTTCCCGGCC CTTTCTGGGC GCATAAGAAC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttc3 ( XM_018091393.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946882.1 Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.

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CloneID	OXa42625
Clone ID Related Accession (Same CDS sequence)	XM_018091393.1
Accession Version	XM_018091393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGAA GAGAAATCCA ATGGCAACTC TCTCCTTGCA GGGTTTTATC 
CGTGTGATAT GTTGCCTGTC ATGTAAAGTG GAGTTCCATG TTTGCTGCTG GAAGAAGCTG 
AAGGCCAACA ACTATTCCGA TAAAGCTGAC AGGGATTTTC TAACTATCTC TTGTTTTACA 
CCTGACTGTA AAGGCAAAAT CTCTAATATA ATTATTTATG ACTCCACTGG ACTTGTAAAA 
CGTGAGTTTG CAGATAAGTC TGTTAAGAAG AAAGAACCAC AAAGGCCAAC AGTTAAGAAT 
AAAGGAAGTA GCAATAAGAA GTTAAAGATT AAGCCTGAGA GTAAAACACA AGCAAAGAAG 
ACCTCACCTG AAGAGAACAG GAGCTTGATA CACAATGAGA AATCACAGAA GCAGCAAGAA 
AATATCTCCT CTAACCAACG GAATAATATA TGTAATAACC GTTATAGTGT AGCACCTCAT 
GATTTAATGA TGACGCTTAT ATTGAATAAG GAGGAGGTTT TTAAAACAGG AGTGGAGAGC 
ACCAAAGCAT TGTGCAACTC ATTAATCTCA TGGAATGTTA TAAGTCAGGA AGAACTTGAT 
GGTTTTTCCT TTGAAACAAC ATGTAGATCG TCTTCTGATG AAATGGCAGT CCTGCTCGCT 
CATCTGTGCA GCTTAAATAA TAGAGTAAAG ACAAGAATAT TCCTCTACCT GCTGGAAGAT 
TATCCTTTAA CTTTAGAACT GCCTGCATTT ATAAAGATGA TTAGAGATAC GGGTCATCGG 
GCAGCAAAGA AGTTCCAAGA GATAAACAGC AGCCTCCTGA AAGAGCTTAA TTTCGAGTTG 
CTAGCCGGTC GGTGGAACGA AACCTACGGA AAACTTTTAA ACTTCTTCAT GTCTATATCT 
GTGGAAACAG CTGAGAAACT AAGTCAAGCA AATATCCAAA TATTCCGCTG CTTCATATGG 
TTTCTAGACG ACAATAGATA TGTCCCCATG CCTGCCGGGC TGGATAAATA TTTAGATAAA 
TATTTTATAG ATATGGATAT ACCATATTCC AAAAATGATG TGCTCGACTC TGTTAATAAA 
GACCCTATAG AAGCCCCTTG TTGCATCAAT ATACGAATGA AGTCAGATGA AGAGCAGCCT 
ACTAAATGTA TCAAGGTTAA GAACAAACAC AAGAAAAAGA AGCAAAATCA GCCCAAGACT 
TTGTATAAGG TTTCTGGTGC AGTAAGTACC AGGCCTCCAG AAGATGACAT TTTTACAGAA 
GAAAATACTC TAAGCCTGTT GGATCCTTAT GAACCCTTTA TAATCCCAGA ATATTTGCGT 
CCTGATGTGG AAGAATTTGA AACCCTTTAT GATCCCACTC CTGGCGGATA CCGGTTACCA 
TGGCAACCTG ATAATGCCAT GCAAGAAACC CTCTATGAAT ATTTTTCTGA GATCCTGGAA 
GAGCATGGGC CTTTGGAACT GGATGATGAC ATTCTGATTG GGACATTTAA GGAGTTCCCA 
GAGGAGGCAC ACCAGCTGGT ACAGGAGGCC GGCAGCCTAA AGGACTTCCT TCTGCAATCA 
ACTAAGTTTG TCATGGTGGG AAAGCTCCTG GGACTGCGCC CTCACAAAGT TATATTGGAA 
GAAGCATCCG GAAAAGCCAA ACTCAACCCA ACCGCTAAAG AATTCCAGCC TTCATCTAAT 
AATCCTTTTG ATTCAATAAT AAGCGAGACT TTGGAAATAA GTCCTGAATA CAATCCACTT 
GAGCCATCCA ATCCCTTTGT TTTACTGCCC AATCAAAGCC CAGTTTTTAA TGGCAATTGT 
ATAGTCCCTT GTCTTCCGCT GTATGGACTG AATCCTTTGC CTGTAGTTTC TGTAGGGCAC 
CCATTTATAC CTGGTCTCGG CATTGGAATG CAGCCTACGA TAACAGTTAA TAGTGCTGTT 
ATGTGTAATT TAGGCGTGCC GCTTCCTAAT AGAGCAAAGG AGCCAGAGCA GTCAAGTCCA 
ATAATGCATA ACACTTTGAC TGGGAATTTG AATCCCAAAA ATGACACTGC ATCAGAGACC 
ACCATGACCC AGACTGTTCC TTTAAGAAGA AATATTCACA CCGGGGTAGT GTCAGTACAG 
GTAGACAGAG ACTTATCTGA GCAAGAAGTA AATACTGAGC CTTATTATCC TTTTGAAGTG 
CAGCAGGGAG ATATCCTACG GATAGAGAAA GAGCATCTCG TTCTACAGGA GCAGCTAAAA 
GAAGCAACAG AGAAGTATGA GAATCTGCAG GGCCGCTACA AGGAGGAAAT CGCTGGGTTG 
CATAAGCAAA TTAGGGAAAC GGCTGACAAT AATAAGATTA TAAAGAAAGA ACTTGAAATG 
TTTAACCACG AGATGGATAA CGACACCAAA AAATGGCAAC TAGAGAAAAA AGAGTATCAT 
GATAAGCTGA GAGTGGTAAA GAGCGGCATT AAGGCGGCAA ATGAAACTGT CGATAAGTAT 
TCAAGGGAGA TTGAAGAAAA GAAACAACAG TATGAAGCAT ACATAGAGAC ATTCGTACAG 
TCTGGTTTTA ATATTGAAAA TGAAAAGGCA AAGTTGGAAA AGCATATTAA AAGCAACGAG 
GATAAGCTCC AGGAAAGCAA GAAGAGAGCT ATGACTGCTG AGGTCCACTT CCTTGAGAAC 
AAAAAACAGA CTGATTTATT AAAGCTGAGG ATTATGGCTG CAAAGGTGGA ACACAGCGCC 
AACAAGTTAA AAGCCAATGG CTCTGCCACA CCTTTTGCAC AAAAAAGCAT AGCGGATTTA 
TCCCCCTCTT ATCTAAAATC TGAAAGTGAG AAAATAAAGT CCCAGTTTGA TGAGAAGATT 
AAATTAGTGG AAAGTGGATA TAAGTTGGCA AATATACAAG CAAACAATCT GGCAGCTCCA 
TTCAATGCAT CTGCTTTTCA AACACCTCTG CTGCCCCCTT CTCCCACCCC CAAGCTCCCT 
ACAACCAAAC CCAGCACCCC GCTGCCAGCT TCCAGTTCTG CACCTCCAGC TTCCCTCAAG 
TCCCCATTAA AGCCGACTCG CGAAACCAAG AAAAGCAAAT CTAAAGATAT TAATAAGGAT 
CCGAAACCAC CACCTCCTGC AATTAATCCA AAGGCCACGG CATTAGCAGG GAAGGGCCCG 
GGAGCACCCG TCATGGAGAA GAAAGGAAAG CCCAACTTAG AGGCACAACA TCAGTCTCCC 
GGAAAGCCGA CTGCGTTTGA TAAGATTATT GAGGAGCTGC ATGAGATTTT TCCTGACTAC 
AAAAGTGCGG AGCTTACCAA CTTCATCAAG GAGCTGCGCA ATAAGACTGG CGGAACACTG 
GCTGGCATGA AGCACGATGA CATTGTTAGC AGGGTGACTG AGTATATACT GGACTCCCAG 
GATAAGGCCC CCCCACAGGC ACCTTGGGTA CAAGCAAGTG GCAATGTATA TCCTTCAGCA 
GTGAAACCCC AGGCCCCATC GCCCCAGCCC AAACAGCCCT GGAGAGTGGT CACTGGTCCC 
TCCAAGAACA AGTGGCAGAA GTCTGATGAT ACAGAGTGCT ATAATGATGA GCCGTGCATT 
ATCTGCCATG ATGAGCTGCA ACAGTATCCG GTGCAGAAGC TGGACTGTGG GCACTGCTTC 
CATAGACACT GCATAAAGAC CTGGCTGAAC ACACAGAGCA CCTGCCCGAC CTGCAGAGAT 
CACGCTCTCC TTCCTGAGGA CTTCCCGGCC CTTTCTGGGC GCATAAGAAC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946882.1
CDS	146..5722
Translation	MDGIGYTDIGNGSSHVIATCFRGHEYPMQRPPVPVGEKVDPVTFWLSHPLDVIHEHCDLIKVYILWPVLKADASPVYKGPWVDHDGSGLCIQELNFVEMLEDLLDVVKKVAHERRFIEGLLKIAKTMEMVQLNYQHLSEAMSWLRSTGELQICKKLEEQDNLSLFPLQILFIDYAHYINSMADQRCSLSFMWKKKHICDCCAVESEGMKQRGNEEFSKSNFCLAADLYSKAIEYSPDNHLLFGNRSLCFLRMGEYMNALSDGKKATVLKHKWPKGHYRFCEALFLMGERERALASNEKAQLLCKDSPEGIRDLIQQHTKIKRKIEEMKDGKHRRSTNKKFIRDKKIAPYSGPMSVLKEKKKIPSSNKEDIPNKESDGATLENKDIISDNVTGQNQKKGKSKGKGGESEKLRVSNAKSDPKENQKDVSCNYSQMDLSTLQDMVKSLVRDGCEALADSRCHNALGYFSKLLDILAASDLQRFYLSNIDYVVLLYGYANALLGTGQCEELTKAEDQLKKIITQFTKERFNCLAFYGIGNVYYRQNRFSDALNQYVKSHTMVKLKMMPGILTWPTTPIVLEESRPEKLQSLLENRIEECKFPPKPDAICRYKQCLSLPKPQIYFTDPDFKKRNPMATLSLQGFIRVICCLSCKVEFHVCCWKKLKANNYSDKADRDFLTISCFTPDCKGKISNIIIYDSTGLVKREFADKSVKKKEPQRPTVKNKGSSNKKLKIKPESKTQAKKTSPEENRSLIHNEKSQKQQENISSNQRNNICNNRYSVAPHDLMMTLILNKEEVFKTGVESTKALCNSLISWNVISQEELDGFSFETTCRSSSDEMAVLLAHLCSLNNRVKTRIFLYLLEDYPLTLELPAFIKMIRDTGHRAAKKFQEINSSLLKELNFELLAGRWNETYGKLLNFFMSISVETAEKLSQANIQIFRCFIWFLDDNRYVPMPAGLDKYLDKYFIDMDIPYSKNDVLDSVNKDPIEAPCCINIRMKSDEEQPTKCIKVKNKHKKKKQNQPKTLYKVSGAVSTRPPEDDIFTEENTLSLLDPYEPFIIPEYLRPDVEEFETLYDPTPGGYRLPWQPDNAMQETLYEYFSEILEEHGPLELDDDILIGTFKEFPEEAHQLVQEAGSLKDFLLQSTKFVMVGKLLGLRPHKVILEEASGKAKLNPTAKEFQPSSNNPFDSIISETLEISPEYNPLEPSNPFVLLPNQSPVFNGNCIVPCLPLYGLNPLPVVSVGHPFIPGLGIGMQPTITVNSAVMCNLGVPLPNRAKEPEQSSPIMHNTLTGNLNPKNDTASETTMTQTVPLRRNIHTGVVSVQVDRDLSEQEVNTEPYYPFEVQQGDILRIEKEHLVLQEQLKEATEKYENLQGRYKEEIAGLHKQIRETADNNKIIKKELEMFNHEMDNDTKKWQLEKKEYHDKLRVVKSGIKAANETVDKYSREIEEKKQQYEAYIETFVQSGFNIENEKAKLEKHIKSNEDKLQESKKRAMTAEVHFLENKKQTDLLKLRIMAAKVEHSANKLKANGSATPFAQKSIADLSPSYLKSESEKIKSQFDEKIKLVESGYKLANIQANNLAAPFNASAFQTPLLPPSPTPKLPTTKPSTPLPASSSAPPASLKSPLKPTRETKKSKSKDINKDPKPPPPAINPKATALAGKGPGAPVMEKKGKPNLEAQHQSPGKPTAFDKIIEELHEIFPDYKSAELTNFIKELRNKTGGTLAGMKHDDIVSRVTEYILDSQDKAPPQAPWVQASGNVYPSAVKPQAPSPQPKQPWRVVTGPSKNKWQKSDDTECYNDEPCIICHDELQQYPVQKLDCGHCFHRHCIKTWLNTQSTCPTCRDHALLPEDFPALSGRIRTT

Target ORF information:

RefSeq Version	XM_018091393.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091393.1

ORF Insert Sequence:

ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGAA GAGAAATCCA ATGGCAACTC TCTCCTTGCA GGGTTTTATC 
CGTGTGATAT GTTGCCTGTC ATGTAAAGTG GAGTTCCATG TTTGCTGCTG GAAGAAGCTG 
AAGGCCAACA ACTATTCCGA TAAAGCTGAC AGGGATTTTC TAACTATCTC TTGTTTTACA 
CCTGACTGTA AAGGCAAAAT CTCTAATATA ATTATTTATG ACTCCACTGG ACTTGTAAAA 
CGTGAGTTTG CAGATAAGTC TGTTAAGAAG AAAGAACCAC AAAGGCCAAC AGTTAAGAAT 
AAAGGAAGTA GCAATAAGAA GTTAAAGATT AAGCCTGAGA GTAAAACACA AGCAAAGAAG 
ACCTCACCTG AAGAGAACAG GAGCTTGATA CACAATGAGA AATCACAGAA GCAGCAAGAA 
AATATCTCCT CTAACCAACG GAATAATATA TGTAATAACC GTTATAGTGT AGCACCTCAT 
GATTTAATGA TGACGCTTAT ATTGAATAAG GAGGAGGTTT TTAAAACAGG AGTGGAGAGC 
ACCAAAGCAT TGTGCAACTC ATTAATCTCA TGGAATGTTA TAAGTCAGGA AGAACTTGAT 
GGTTTTTCCT TTGAAACAAC ATGTAGATCG TCTTCTGATG AAATGGCAGT CCTGCTCGCT 
CATCTGTGCA GCTTAAATAA TAGAGTAAAG ACAAGAATAT TCCTCTACCT GCTGGAAGAT 
TATCCTTTAA CTTTAGAACT GCCTGCATTT ATAAAGATGA TTAGAGATAC GGGTCATCGG 
GCAGCAAAGA AGTTCCAAGA GATAAACAGC AGCCTCCTGA AAGAGCTTAA TTTCGAGTTG 
CTAGCCGGTC GGTGGAACGA AACCTACGGA AAACTTTTAA ACTTCTTCAT GTCTATATCT 
GTGGAAACAG CTGAGAAACT AAGTCAAGCA AATATCCAAA TATTCCGCTG CTTCATATGG 
TTTCTAGACG ACAATAGATA TGTCCCCATG CCTGCCGGGC TGGATAAATA TTTAGATAAA 
TATTTTATAG ATATGGATAT ACCATATTCC AAAAATGATG TGCTCGACTC TGTTAATAAA 
GACCCTATAG AAGCCCCTTG TTGCATCAAT ATACGAATGA AGTCAGATGA AGAGCAGCCT 
ACTAAATGTA TCAAGGTTAA GAACAAACAC AAGAAAAAGA AGCAAAATCA GCCCAAGACT 
TTGTATAAGG TTTCTGGTGC AGTAAGTACC AGGCCTCCAG AAGATGACAT TTTTACAGAA 
GAAAATACTC TAAGCCTGTT GGATCCTTAT GAACCCTTTA TAATCCCAGA ATATTTGCGT 
CCTGATGTGG AAGAATTTGA AACCCTTTAT GATCCCACTC CTGGCGGATA CCGGTTACCA 
TGGCAACCTG ATAATGCCAT GCAAGAAACC CTCTATGAAT ATTTTTCTGA GATCCTGGAA 
GAGCATGGGC CTTTGGAACT GGATGATGAC ATTCTGATTG GGACATTTAA GGAGTTCCCA 
GAGGAGGCAC ACCAGCTGGT ACAGGAGGCC GGCAGCCTAA AGGACTTCCT TCTGCAATCA 
ACTAAGTTTG TCATGGTGGG AAAGCTCCTG GGACTGCGCC CTCACAAAGT TATATTGGAA 
GAAGCATCCG GAAAAGCCAA ACTCAACCCA ACCGCTAAAG AATTCCAGCC TTCATCTAAT 
AATCCTTTTG ATTCAATAAT AAGCGAGACT TTGGAAATAA GTCCTGAATA CAATCCACTT 
GAGCCATCCA ATCCCTTTGT TTTACTGCCC AATCAAAGCC CAGTTTTTAA TGGCAATTGT 
ATAGTCCCTT GTCTTCCGCT GTATGGACTG AATCCTTTGC CTGTAGTTTC TGTAGGGCAC 
CCATTTATAC CTGGTCTCGG CATTGGAATG CAGCCTACGA TAACAGTTAA TAGTGCTGTT 
ATGTGTAATT TAGGCGTGCC GCTTCCTAAT AGAGCAAAGG AGCCAGAGCA GTCAAGTCCA 
ATAATGCATA ACACTTTGAC TGGGAATTTG AATCCCAAAA ATGACACTGC ATCAGAGACC 
ACCATGACCC AGACTGTTCC TTTAAGAAGA AATATTCACA CCGGGGTAGT GTCAGTACAG 
GTAGACAGAG ACTTATCTGA GCAAGAAGTA AATACTGAGC CTTATTATCC TTTTGAAGTG 
CAGCAGGGAG ATATCCTACG GATAGAGAAA GAGCATCTCG TTCTACAGGA GCAGCTAAAA 
GAAGCAACAG AGAAGTATGA GAATCTGCAG GGCCGCTACA AGGAGGAAAT CGCTGGGTTG 
CATAAGCAAA TTAGGGAAAC GGCTGACAAT AATAAGATTA TAAAGAAAGA ACTTGAAATG 
TTTAACCACG AGATGGATAA CGACACCAAA AAATGGCAAC TAGAGAAAAA AGAGTATCAT 
GATAAGCTGA GAGTGGTAAA GAGCGGCATT AAGGCGGCAA ATGAAACTGT CGATAAGTAT 
TCAAGGGAGA TTGAAGAAAA GAAACAACAG TATGAAGCAT ACATAGAGAC ATTCGTACAG 
TCTGGTTTTA ATATTGAAAA TGAAAAGGCA AAGTTGGAAA AGCATATTAA AAGCAACGAG 
GATAAGCTCC AGGAAAGCAA GAAGAGAGCT ATGACTGCTG AGGTCCACTT CCTTGAGAAC 
AAAAAACAGA CTGATTTATT AAAGCTGAGG ATTATGGCTG CAAAGGTGGA ACACAGCGCC 
AACAAGTTAA AAGCCAATGG CTCTGCCACA CCTTTTGCAC AAAAAAGCAT AGCGGATTTA 
TCCCCCTCTT ATCTAAAATC TGAAAGTGAG AAAATAAAGT CCCAGTTTGA TGAGAAGATT 
AAATTAGTGG AAAGTGGATA TAAGTTGGCA AATATACAAG CAAACAATCT GGCAGCTCCA 
TTCAATGCAT CTGCTTTTCA AACACCTCTG CTGCCCCCTT CTCCCACCCC CAAGCTCCCT 
ACAACCAAAC CCAGCACCCC GCTGCCAGCT TCCAGTTCTG CACCTCCAGC TTCCCTCAAG 
TCCCCATTAA AGCCGACTCG CGAAACCAAG AAAAGCAAAT CTAAAGATAT TAATAAGGAT 
CCGAAACCAC CACCTCCTGC AATTAATCCA AAGGCCACGG CATTAGCAGG GAAGGGCCCG 
GGAGCACCCG TCATGGAGAA GAAAGGAAAG CCCAACTTAG AGGCACAACA TCAGTCTCCC 
GGAAAGCCGA CTGCGTTTGA TAAGATTATT GAGGAGCTGC ATGAGATTTT TCCTGACTAC 
AAAAGTGCGG AGCTTACCAA CTTCATCAAG GAGCTGCGCA ATAAGACTGG CGGAACACTG 
GCTGGCATGA AGCACGATGA CATTGTTAGC AGGGTGACTG AGTATATACT GGACTCCCAG 
GATAAGGCCC CCCCACAGGC ACCTTGGGTA CAAGCAAGTG GCAATGTATA TCCTTCAGCA 
GTGAAACCCC AGGCCCCATC GCCCCAGCCC AAACAGCCCT GGAGAGTGGT CACTGGTCCC 
TCCAAGAACA AGTGGCAGAA GTCTGATGAT ACAGAGTGCT ATAATGATGA GCCGTGCATT 
ATCTGCCATG ATGAGCTGCA ACAGTATCCG GTGCAGAAGC TGGACTGTGG GCACTGCTTC 
CATAGACACT GCATAAAGAC CTGGCTGAAC ACACAGAGCA CCTGCCCGAC CTGCAGAGAT 
CACGCTCTCC TTCCTGAGGA CTTCCCGGCC CTTTCTGGGC GCATAAGAAC CACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttc3 ( XM_004912114.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912171.2 Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

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CloneID	OXa51934
Clone ID Related Accession (Same CDS sequence)	XM_004912114.4
Accession Version	XM_004912114.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912114.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA AACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912171.2
CDS	91..5754
Translation	MGVPGRYCYFPLSRSRIWAGGLPCSFSISAPWDCGWDPSAMDGIGYTDIGNGSSHVIATCFRGHEYPMQRPPVPVGEKVDPVTFWLSHPLDVIHEHCDLIKVYILWPVLKADASPVYKGPWVDHDGSGLCIQELNFVEMLEDLLDVVKKVAHERRFIEGLLKIAKTMEMVQLNYQHLSEAMSWLRSTGELQICKKLEEQDNLSLFPLQILFIDYAHYINSMADQRCSLSFMWKKKHICDCCAVESEGMKQRGNEEFSKSNFCLAADLYSKAIEYSPDNHLLFGNRSLCFLRMGEYMNALSDGKKATVLKHKWPKGHYRFCEALFLMGERERALASNEKAQLLCKDSPEGIRDLIQQQTKIKRKIEEMKDGKHRRSTNKKFIRDKKIAPYSGPMSVLKEKKKIPSSNKEDIPNKESDGATLENKDIISDNVTGQNQKKGKSKGKGGESEKLRVSNAKSDPKENQKDVSCNYSQMDLSTLQDMVKSLVRDGCEALADSRCHNALGYFSKLLDILAASDLQRFYLSNIDYVVLLYGYANALLGTGQCEELTKAEDQLKKIITQFTKERFNCLAFYGIGNVYYRQNRFSDALNQYVKSHTMVKLKMMPGILTWPTTPIVLEESRPEKLQSLLENRIEECKFPPKPDAICRYKQCLSLPKPQIYFTDPDFKGFIRVICCLSCKVEFHVCCWKKLKANNYSDKADRDFLTISCFTPDCKGKISNIIIYDSTGLVKREFADKSVKKKEPQRPTVKNKGSSNKKLKIKPESKTQAKKTSPEENRSLIHNEKSQKQQENISSNQRNNICNNRYSVAPHDLMMTLILNKEEVFKTGVESTKALCNSLISWNVISQEELDGFSFETTCRSSSDEMAVLLAHLCSLNNRVKTRIFLYLLEDYPLTLELPAFIKMIRDTGHRAAKKFQEINSSLLKELNFELLAGRWNETYGKLLNFFMSISVETAEKLSQANIQIFRCFIWFLDDNRYVPMPAGLDKYLDKYFIDMDIPYSKNDVLDSVNKDPIEAPCCINIRMKSDEEQPTKCIKVKNKHKKKKQNQPKTLYKVSGAVSTRPPEDDIFTEENTLSLLDPYEPFIIPEYLRPDVEEFETLYDPTPGGYRLPWQPDNAMQETLYEYFSEILEEHGPLELDDDILIGTFKEFPEEAHQLVQEAGSLKDFLLQSTKFVMVGKLLGLRPHKVILEEASGKAKLNPTAKEFQPSSNNPFDSIISETLEISPEYNPLEPSNPFVLLPNQSPVFNGNCIVPCLPLYGLNPLPVVSVGHPFIPGLGIGMQPTITVNSAVMCNLGVPLPNRAKEPEQSSPIMHNTLTGNLNPKNDTASETTMTQTVPLRRNIHTGVVSVQVDRDLSEQEVNTEPYYPFEVQQGDILRIEKEHLVLQEQLKEATEKYENLQGRYKEEIAGLHKQIRETADNNKIIKKELEMFNHEMDNDTKKWQLEKKEYHDKLRVVKSGIKAANETVDKYSREIEEKKQQYEAYIETFVQSGFNIENEKAKLEKHIKSNEDKLQESKKRAMTAEVHFLENKKQTDLLKLRIMAAKVEHSANKLKANGSATPFAQKSIADLSPSYLKSESEKIKSQFDEKIKLVESGYKLANIQANNLAAPFNASAFQTPLLPPSPTPKLPTTKPSTPLPASSSAPPASLKSPLKPTRETKKSKSKDINKDPKPPPPAINPKATALAGKGPGAPVMEKKGKPNLEAQHQSPGKPTAFDKIIEELHEIFPDYKSAELTNFIKELRNKTGGTLAGMKHDDIVSRVTEYILDSQDKAPPQAPWVQASGNVYPSAVKPQAPSPQPKQPWRVVTGPSKNKWQKSDDTECYNDEPCIICHDELQQYPVQKLDCGHCFHRHCIKTWLNTQSTCPTCRDHALLPEDFPALSGRIRTT

Target ORF information:

RefSeq Version	XM_004912114.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912114.4

ORF Insert Sequence:

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA AACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttc3 ( XM_031896550.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752410.1 Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa51935
Clone ID Related Accession (Same CDS sequence)	XM_031896550.1
Accession Version	XM_031896550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA AACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752410.1
CDS	152..5695
Translation	MDGIGYTDIGNGSSHVIATCFRGHEYPMQRPPVPVGEKVDPVTFWLSHPLDVIHEHCDLIKVYILWPVLKADASPVYKGPWVDHDGSGLCIQELNFVEMLEDLLDVVKKVAHERRFIEGLLKIAKTMEMVQLNYQHLSEAMSWLRSTGELQICKKLEEQDNLSLFPLQILFIDYAHYINSMADQRCSLSFMWKKKHICDCCAVESEGMKQRGNEEFSKSNFCLAADLYSKAIEYSPDNHLLFGNRSLCFLRMGEYMNALSDGKKATVLKHKWPKGHYRFCEALFLMGERERALASNEKAQLLCKDSPEGIRDLIQQQTKIKRKIEEMKDGKHRRSTNKKFIRDKKIAPYSGPMSVLKEKKKIPSSNKEDIPNKESDGATLENKDIISDNVTGQNQKKGKSKGKGGESEKLRVSNAKSDPKENQKDVSCNYSQMDLSTLQDMVKSLVRDGCEALADSRCHNALGYFSKLLDILAASDLQRFYLSNIDYVVLLYGYANALLGTGQCEELTKAEDQLKKIITQFTKERFNCLAFYGIGNVYYRQNRFSDALNQYVKSHTMVKLKMMPGILTWPTTPIVLEESRPEKLQSLLENRIEECKFPPKPDAICRYKQCLSLPKPQIYFTDPDFKGFIRVICCLSCKVEFHVCCWKKLKANNYSDKADRDFLTISCFTPDCKGKISNIIIYDSTGLVKREFADKSVKKKEPQRPTVKNKGSSNKKLKIKPESKTQAKKTSPEENRSLIHNEKSQKQQENISSNQRNNICNNRYSVAPHDLMMTLILNKEEVFKTGVESTKALCNSLISWNVISQEELDGFSFETTCRSSSDEMAVLLAHLCSLNNRVKTRIFLYLLEDYPLTLELPAFIKMIRDTGHRAAKKFQEINSSLLKELNFELLAGRWNETYGKLLNFFMSISVETAEKLSQANIQIFRCFIWFLDDNRYVPMPAGLDKYLDKYFIDMDIPYSKNDVLDSVNKDPIEAPCCINIRMKSDEEQPTKCIKVKNKHKKKKQNQPKTLYKVSGAVSTRPPEDDIFTEENTLSLLDPYEPFIIPEYLRPDVEEFETLYDPTPGGYRLPWQPDNAMQETLYEYFSEILEEHGPLELDDDILIGTFKEFPEEAHQLVQEAGSLKDFLLQSTKFVMVGKLLGLRPHKVILEEASGKAKLNPTAKEFQPSSNNPFDSIISETLEISPEYNPLEPSNPFVLLPNQSPVFNGNCIVPCLPLYGLNPLPVVSVGHPFIPGLGIGMQPTITVNSAVMCNLGVPLPNRAKEPEQSSPIMHNTLTGNLNPKNDTASETTMTQTVPLRRNIHTGVVSVQVDRDLSEQEVNTEPYYPFEVQQGDILRIEKEHLVLQEQLKEATEKYENLQGRYKEEIAGLHKQIRETADNNKIIKKELEMFNHEMDNDTKKWQLEKKEYHDKLRVVKSGIKAANETVDKYSREIEEKKQQYEAYIETFVQSGFNIENEKAKLEKHIKSNEDKLQESKKRAMTAEVHFLENKKQTDLLKLRIMAAKVEHSANKLKANGSATPFAQKSIADLSPSYLKSESEKIKSQFDEKIKLVESGYKLANIQANNLAAPFNASAFQTPLLPPSPTPKLPTTKPSTPLPASSSAPPASLKSPLKPTRETKKSKSKDINKDPKPPPPAINPKATALAGKGPGAPVMEKKGKPNLEAQHQSPGKPTAFDKIIEELHEIFPDYKSAELTNFIKELRNKTGGTLAGMKHDDIVSRVTEYILDSQDKAPPQAPWVQASGNVYPSAVKPQAPSPQPKQPWRVVTGPSKNKWQKSDDTECYNDEPCIICHDELQQYPVQKLDCGHCFHRHCIKTWLNTQSTCPTCRDHALLPEDFPALSGRIRTT

Target ORF information:

RefSeq Version	XM_031896550.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896550.1

ORF Insert Sequence:

ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA AACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttc3 ( XM_004912114.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912171.1 Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

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CloneID	OXa06400
Clone ID Related Accession (Same CDS sequence)	XM_004912114.3
Accession Version	XM_004912114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912114.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912171.1
CDS	133..5796
Translation	MGVPGRYCYFPLSRSRIWAGGLPCSFSISAPWDCGWDPSAMDGIGYTDIGNGSSHVIATCFRGHEYPMQRPPVPVGEKVDPVTFWLSHPLDVIHEHCDLIKVYILWPVLKADASPVYKGPWVDHDGSGLCIQELNFVEMLEDLLDVVKKVAHERRFIEGLLKIAKTMEMVQLNYQHLSEAMSWLRSTGELQICKKLEEQDNLSLFPLQILFIDYAHYINSMADQRCSLSFMWKKKHICDCCAVESEGMKQRGNEEFSKSNFCLAADLYSKAIEYSPDNHLLFGNRSLCFLRMGEYMNALSDGKKATVLKHKWPKGHYRFCEALFLMGERERALASNEKAQLLCKDSPEGIRDLIQQHTKIKRKIEEMKDGKHRRSTNKKFIRDKKIAPYSGPMSVLKEKKKIPSSNKEDIPNKESDGATLENKDIISDNVTGQNQKKGKSKGKGGESEKLRVSNAKSDPKENQKDVSCNYSQMDLSTLQDMVKSLVRDGCEALADSRCHNALGYFSKLLDILAASDLQRFYLSNIDYVVLLYGYANALLGTGQCEELTKAEDQLKKIITQFTKERFNCLAFYGIGNVYYRQNRFSDALNQYVKSHTMVKLKMMPGILTWPTTPIVLEESRPEKLQSLLENRIEECKFPPKPDAICRYKQCLSLPKPQIYFTDPDFKGFIRVICCLSCKVEFHVCCWKKLKANNYSDKADRDFLTISCFTPDCKGKISNIIIYDSTGLVKREFADKSVKKKEPQRPTVKNKGSSNKKLKIKPESKTQAKKTSPEENRSLIHNEKSQKQQENISSNQRNNICNNRYSVAPHDLMMTLILNKEEVFKTGVESTKALCNSLISWNVISQEELDGFSFETTCRSSSDEMAVLLAHLCSLNNRVKTRIFLYLLEDYPLTLELPAFIKMIRDTGHRAAKKFQEINSSLLKELNFELLAGRWNETYGKLLNFFMSISVETAEKLSQANIQIFRCFIWFLDDNRYVPMPAGLDKYLDKYFIDMDIPYSKNDVLDSVNKDPIEAPCCINIRMKSDEEQPTKCIKVKNKHKKKKQNQPKTLYKVSGAVSTRPPEDDIFTEENTLSLLDPYEPFIIPEYLRPDVEEFETLYDPTPGGYRLPWQPDNAMQETLYEYFSEILEEHGPLELDDDILIGTFKEFPEEAHQLVQEAGSLKDFLLQSTKFVMVGKLLGLRPHKVILEEASGKAKLNPTAKEFQPSSNNPFDSIISETLEISPEYNPLEPSNPFVLLPNQSPVFNGNCIVPCLPLYGLNPLPVVSVGHPFIPGLGIGMQPTITVNSAVMCNLGVPLPNRAKEPEQSSPIMHNTLTGNLNPKNDTASETTMTQTVPLRRNIHTGVVSVQVDRDLSEQEVNTEPYYPFEVQQGDILRIEKEHLVLQEQLKEATEKYENLQGRYKEEIAGLHKQIRETADNNKIIKKELEMFNHEMDNDTKKWQLEKKEYHDKLRVVKSGIKAANETVDKYSREIEEKKQQYEAYIETFVQSGFNIENEKAKLEKHIKSNEDKLQESKKRAMTAEVHFLENKKQTDLLKLRIMAAKVEHSANKLKANGSATPFAQKSIADLSPSYLKSESEKIKSQFDEKIKLVESGYKLANIQANNLAAPFNASAFQTPLLPPSPTPKLPTTKPSTPLPASSSAPPASLKSPLKPTRETKKSKSKDINKDPKPPPPAINPKATALAGKGPGAPVMEKKGKPNLEAQHQSPGKPTAFDKIIEELHEIFPDYKSAELTNFIKELRNKTGGTLAGMKHDDIVSRVTEYILDSQDKAPPQAPWVQASGNVYPSAVKPQAPSPQPKQPWRVVTGPSKNKWQKSDDTECYNDEPCIICHDELQQYPVQKLDCGHCFHRHCIKTWLNTQSTCPTCRDHALLPEDFPALSGRIRTT

Target ORF information:

RefSeq Version	XM_004912114.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912114.3

ORF Insert Sequence:

ATGGGGGTTC CGGGGCGCTA TTGCTACTTC CCCCTGAGTA GAAGCCGGAT ATGGGCAGGA 
GGCTTGCCTT GCTCCTTCAG CATCAGTGCC CCCTGGGATT GCGGCTGGGA CCCTTCAGCA 
ATGGATGGGA TCGGGTACAC AGATATTGGC AATGGAAGCT CGCACGTTAT TGCCACCTGT 
TTCCGAGGTC ATGAATATCC TATGCAAAGA CCACCTGTAC CTGTTGGGGA GAAAGTCGAT 
CCTGTTACAT TCTGGCTCTC TCATCCATTA GACGTTATAC ATGAACACTG TGATTTAATT 
AAAGTCTATA TTTTGTGGCC AGTCCTTAAG GCAGATGCAT CTCCAGTGTA CAAAGGACCA 
TGGGTTGATC ATGATGGTTC TGGCCTGTGT ATTCAAGAAC TAAACTTCGT TGAGATGTTA 
GAAGATCTCC TGGATGTAGT AAAGAAAGTG GCGCATGAGC GACGGTTTAT AGAGGGCTTA 
TTAAAGATTG CGAAGACAAT GGAAATGGTT CAGTTAAATT ATCAGCATCT ATCAGAAGCA 
ATGAGCTGGC TGAGATCGAC AGGGGAACTA CAAATTTGCA AGAAACTGGA AGAACAAGAT 
AATTTAAGCC TCTTTCCTCT GCAGATATTA TTTATTGACT ATGCACATTA CATAAACAGT 
ATGGCTGACC AGAGGTGCAG TTTATCCTTT ATGTGGAAAA AAAAACATAT TTGTGACTGC 
TGTGCTGTGG AGTCAGAAGG TATGAAGCAA CGTGGTAATG AAGAGTTTTC TAAATCAAAT 
TTTTGCTTGG CTGCTGATCT GTATTCTAAA GCCATAGAAT ATAGTCCTGA TAATCATCTT 
CTATTTGGCA ACAGATCTCT CTGCTTCTTG CGTATGGGCG AATACATGAA TGCTCTGTCT 
GATGGCAAGA AGGCTACAGT TCTCAAGCAT AAATGGCCAA AGGGTCACTA CCGTTTCTGT 
GAAGCCCTGT TTCTTATGGG AGAGCGAGAG CGAGCGCTTG CTTCCAACGA GAAGGCTCAG 
CTGCTTTGCA AGGACAGCCC TGAAGGCATC AGAGACTTAA TTCAGCAACA TACAAAGATC 
AAGAGAAAAA TAGAGGAGAT GAAAGATGGA AAGCATCGGA GATCTACTAA CAAAAAGTTT 
ATCCGTGACA AAAAGATTGC ACCCTATTCG GGTCCCATGT CAGTCTTGAA GGAAAAAAAG 
AAAATACCAT CTAGTAATAA AGAAGATATC CCCAATAAGG AAAGTGATGG GGCCACTTTA 
GAAAATAAGG ACATTATTTC TGATAATGTA ACTGGGCAAA ATCAGAAGAA GGGCAAATCA 
AAGGGGAAAG GGGGAGAATC TGAAAAATTA AGGGTATCTA ATGCAAAGTC TGATCCTAAG 
GAAAACCAGA AGGATGTATC ATGTAATTAT TCACAGATGG ATCTATCAAC TCTGCAAGAC 
ATGGTTAAGT CGTTAGTGCG CGATGGGTGT GAGGCGCTTG CAGACAGCCG GTGCCATAAT 
GCACTAGGAT ATTTTTCTAA ATTGTTGGAT ATCTTGGCTG CATCTGACCT CCAGAGATTC 
TATCTATCTA ATATAGACTA TGTGGTGCTC CTCTACGGCT ATGCTAATGC ACTGCTTGGC 
ACTGGGCAAT GTGAGGAGCT GACCAAAGCT GAAGACCAGC TGAAGAAAAT TATAACTCAG 
TTCACCAAAG AGAGGTTTAA CTGCTTGGCT TTTTATGGCA TCGGCAATGT ATATTATAGG 
CAAAATAGAT TTTCTGATGC ATTAAATCAG TACGTCAAAT CACATACTAT GGTAAAGCTC 
AAGATGATGC CAGGGATTCT AACGTGGCCG ACTACACCTA TAGTCCTTGA AGAGTCAAGG 
CCTGAAAAAC TGCAGTCACT TTTAGAAAAC CGTATAGAAG AATGCAAGTT TCCTCCAAAG 
CCAGATGCCA TTTGTCGGTA TAAGCAATGC CTTAGTTTGC CAAAACCGCA GATATATTTT 
ACGGATCCTG ATTTCAAGGG TTTTATCCGT GTGATATGTT GCCTGTCATG TAAAGTGGAG 
TTCCATGTTT GCTGCTGGAA GAAGCTGAAG GCCAACAACT ATTCCGATAA AGCTGACAGG 
GATTTTCTAA CTATCTCTTG TTTTACACCT GACTGTAAAG GCAAAATCTC TAATATAATT 
ATTTATGACT CCACTGGACT TGTAAAACGT GAGTTTGCAG ATAAGTCTGT TAAGAAGAAA 
GAACCACAAA GGCCAACAGT TAAGAATAAA GGAAGTAGCA ATAAGAAGTT AAAGATTAAG 
CCTGAGAGTA AAACACAAGC AAAGAAGACC TCACCTGAAG AGAACAGGAG CTTGATACAC 
AATGAGAAAT CACAGAAGCA GCAAGAAAAT ATCTCCTCTA ACCAACGGAA TAATATATGT 
AATAACCGTT ATAGTGTAGC ACCTCATGAT TTAATGATGA CGCTTATATT GAATAAGGAG 
GAGGTTTTTA AAACAGGAGT GGAGAGCACC AAAGCATTGT GCAACTCATT AATCTCATGG 
AATGTTATAA GTCAGGAAGA ACTTGATGGT TTTTCCTTTG AAACAACATG TAGATCGTCT 
TCTGATGAAA TGGCAGTCCT GCTCGCTCAT CTGTGCAGCT TAAATAATAG AGTAAAGACA 
AGAATATTCC TCTACCTGCT GGAAGATTAT CCTTTAACTT TAGAACTGCC TGCATTTATA 
AAGATGATTA GAGATACGGG TCATCGGGCA GCAAAGAAGT TCCAAGAGAT AAACAGCAGC 
CTCCTGAAAG AGCTTAATTT CGAGTTGCTA GCCGGTCGGT GGAACGAAAC CTACGGAAAA 
CTTTTAAACT TCTTCATGTC TATATCTGTG GAAACAGCTG AGAAACTAAG TCAAGCAAAT 
ATCCAAATAT TCCGCTGCTT CATATGGTTT CTAGACGACA ATAGATATGT CCCCATGCCT 
GCCGGGCTGG ATAAATATTT AGATAAATAT TTTATAGATA TGGATATACC ATATTCCAAA 
AATGATGTGC TCGACTCTGT TAATAAAGAC CCTATAGAAG CCCCTTGTTG CATCAATATA 
CGAATGAAGT CAGATGAAGA GCAGCCTACT AAATGTATCA AGGTTAAGAA CAAACACAAG 
AAAAAGAAGC AAAATCAGCC CAAGACTTTG TATAAGGTTT CTGGTGCAGT AAGTACCAGG 
CCTCCAGAAG ATGACATTTT TACAGAAGAA AATACTCTAA GCCTGTTGGA TCCTTATGAA 
CCCTTTATAA TCCCAGAATA TTTGCGTCCT GATGTGGAAG AATTTGAAAC CCTTTATGAT 
CCCACTCCTG GCGGATACCG GTTACCATGG CAACCTGATA ATGCCATGCA AGAAACCCTC 
TATGAATATT TTTCTGAGAT CCTGGAAGAG CATGGGCCTT TGGAACTGGA TGATGACATT 
CTGATTGGGA CATTTAAGGA GTTCCCAGAG GAGGCACACC AGCTGGTACA GGAGGCCGGC 
AGCCTAAAGG ACTTCCTTCT GCAATCAACT AAGTTTGTCA TGGTGGGAAA GCTCCTGGGA 
CTGCGCCCTC ACAAAGTTAT ATTGGAAGAA GCATCCGGAA AAGCCAAACT CAACCCAACC 
GCTAAAGAAT TCCAGCCTTC ATCTAATAAT CCTTTTGATT CAATAATAAG CGAGACTTTG 
GAAATAAGTC CTGAATACAA TCCACTTGAG CCATCCAATC CCTTTGTTTT ACTGCCCAAT 
CAAAGCCCAG TTTTTAATGG CAATTGTATA GTCCCTTGTC TTCCGCTGTA TGGACTGAAT 
CCTTTGCCTG TAGTTTCTGT AGGGCACCCA TTTATACCTG GTCTCGGCAT TGGAATGCAG 
CCTACGATAA CAGTTAATAG TGCTGTTATG TGTAATTTAG GCGTGCCGCT TCCTAATAGA 
GCAAAGGAGC CAGAGCAGTC AAGTCCAATA ATGCATAACA CTTTGACTGG GAATTTGAAT 
CCCAAAAATG ACACTGCATC AGAGACCACC ATGACCCAGA CTGTTCCTTT AAGAAGAAAT 
ATTCACACCG GGGTAGTGTC AGTACAGGTA GACAGAGACT TATCTGAGCA AGAAGTAAAT 
ACTGAGCCTT ATTATCCTTT TGAAGTGCAG CAGGGAGATA TCCTACGGAT AGAGAAAGAG 
CATCTCGTTC TACAGGAGCA GCTAAAAGAA GCAACAGAGA AGTATGAGAA TCTGCAGGGC 
CGCTACAAGG AGGAAATCGC TGGGTTGCAT AAGCAAATTA GGGAAACGGC TGACAATAAT 
AAGATTATAA AGAAAGAACT TGAAATGTTT AACCACGAGA TGGATAACGA CACCAAAAAA 
TGGCAACTAG AGAAAAAAGA GTATCATGAT AAGCTGAGAG TGGTAAAGAG CGGCATTAAG 
GCGGCAAATG AAACTGTCGA TAAGTATTCA AGGGAGATTG AAGAAAAGAA ACAACAGTAT 
GAAGCATACA TAGAGACATT CGTACAGTCT GGTTTTAATA TTGAAAATGA AAAGGCAAAG 
TTGGAAAAGC ATATTAAAAG CAACGAGGAT AAGCTCCAGG AAAGCAAGAA GAGAGCTATG 
ACTGCTGAGG TCCACTTCCT TGAGAACAAA AAACAGACTG ATTTATTAAA GCTGAGGATT 
ATGGCTGCAA AGGTGGAACA CAGCGCCAAC AAGTTAAAAG CCAATGGCTC TGCCACACCT 
TTTGCACAAA AAAGCATAGC GGATTTATCC CCCTCTTATC TAAAATCTGA AAGTGAGAAA 
ATAAAGTCCC AGTTTGATGA GAAGATTAAA TTAGTGGAAA GTGGATATAA GTTGGCAAAT 
ATACAAGCAA ACAATCTGGC AGCTCCATTC AATGCATCTG CTTTTCAAAC ACCTCTGCTG 
CCCCCTTCTC CCACCCCCAA GCTCCCTACA ACCAAACCCA GCACCCCGCT GCCAGCTTCC 
AGTTCTGCAC CTCCAGCTTC CCTCAAGTCC CCATTAAAGC CGACTCGCGA AACCAAGAAA 
AGCAAATCTA AAGATATTAA TAAGGATCCG AAACCACCAC CTCCTGCAAT TAATCCAAAG 
GCCACGGCAT TAGCAGGGAA GGGCCCGGGA GCACCCGTCA TGGAGAAGAA AGGAAAGCCC 
AACTTAGAGG CACAACATCA GTCTCCCGGA AAGCCGACTG CGTTTGATAA GATTATTGAG 
GAGCTGCATG AGATTTTTCC TGACTACAAA AGTGCGGAGC TTACCAACTT CATCAAGGAG 
CTGCGCAATA AGACTGGCGG AACACTGGCT GGCATGAAGC ACGATGACAT TGTTAGCAGG 
GTGACTGAGT ATATACTGGA CTCCCAGGAT AAGGCCCCCC CACAGGCACC TTGGGTACAA 
GCAAGTGGCA ATGTATATCC TTCAGCAGTG AAACCCCAGG CCCCATCGCC CCAGCCCAAA 
CAGCCCTGGA GAGTGGTCAC TGGTCCCTCC AAGAACAAGT GGCAGAAGTC TGATGATACA 
GAGTGCTATA ATGATGAGCC GTGCATTATC TGCCATGATG AGCTGCAACA GTATCCGGTG 
CAGAAGCTGG ACTGTGGGCA CTGCTTCCAT AGACACTGCA TAAAGACCTG GCTGAACACA 
CAGAGCACCT GCCCGACCTG CAGAGATCAC GCTCTCCTTC CTGAGGACTT CCCGGCCCTT 
TCTGGGCGCA TAAGAACCAC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ttc3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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