TTC3 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015301440.1	XP_015156926.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_416727.5	XP_416727.4	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_015301422.1	XP_015156908.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_015301429.1	XP_015156915.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_416727.6	XP_416727.4	E3 ubiquitin-protein ligase TTC3 isoform X1
XM_015301408.2	XP_015156894.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_015301416.2	XP_015156902.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_015301429.2	XP_015156915.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_025145271.1	XP_025001039.1	E3 ubiquitin-protein ligase TTC3 isoform X6
XM_015301408.1	XP_015156894.1	E3 ubiquitin-protein ligase TTC3 isoform X2
XM_015301416.1	XP_015156902.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_015301434.1	XP_015156920.1	E3 ubiquitin-protein ligase TTC3 isoform X4
XM_015301434.2	XP_015156920.1	E3 ubiquitin-protein ligase TTC3 isoform X5
XM_025145273.1	XP_025001041.1	E3 ubiquitin-protein ligase TTC3 isoform X6
XM_015301422.2	XP_015156908.1	E3 ubiquitin-protein ligase TTC3 isoform X3
XM_025145266.1	XP_025001034.1	E3 ubiquitin-protein ligase TTC3 isoform X4
XM_015301440.2	XP_015156926.1	E3 ubiquitin-protein ligase TTC3 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa142940	XM_025145266.1 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa142941	XM_025145273.1 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa142941	XM_025145271.1 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa30356	XM_015301408.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30357	XM_015301429.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30357	XM_015301416.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30357	XM_015301422.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30358	XM_015301434.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30359	XM_015301440.2 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa02730	XM_416727.6 Latest version!	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OGa02730	XM_416727.5	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa30357	XM_015301429.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30356	XM_015301408.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30357	XM_015301416.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30358	XM_015301434.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30357	XM_015301422.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa30359	XM_015301440.1	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

TTC3 ( XM_025145266.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001034.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa142940
Clone ID Related Accession (Same CDS sequence)	XM_025145266.1
Accession Version	XM_025145266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGC AGTTTACAGA TTTTAGACTG GAATTGGAAG 
AAACTCTGTG ATTTAGAGCT TGTGGAAGAC CTTGTTGATT TGACTAAAAA AGTGGTTAAT 
GTTCCACATT TTATTGGGGG CATACTGAGA ATTGGCAGCA GGTTAGAAAA CGGTTTCTTC 
GATGTTAGGG ATGCACTTGA TTGGATCAGA TGTACAGGTG ATATCAATAT TCTGCAGAAG 
CTGGAGAAAC TTGGTGATTT TGGTTGGCTC TATCTTGAAA CTTTCTTTGG TGAATGCAAA 
CATTATATTA CTAAAGTTGC TTTGGAAGAT TGTAATCTAG TTGAAGAATT TAAAGCTGTC 
ACCTGTGAAA ACTGCAGGAA GAAGAGTGAA TCTATGAAAC AGAAAGGAAA TGAGGAGTTC 
TCCCAAGGAA AATATGATCA TGCTATAATA TCATATACTA AAGCCATAGA GTTTTGTCCT 
GCAAACCATC TTCTGTATGG AAACCGAGCT CTTTGTTTAA TTCTCACGAA GCAGTACAAG 
AGAGCCCTTG CTGATGGAAA AAGGGCCACT ATTTTGAAGC CTAATTGGCC AAAGGGTCAT 
TACCACTTCT GTAAGGCGCT GTCATTACTG GGGGAGCATG AACTGGCTTT AGAAGCTAAT 
GAGAGAGCTC AGGAACTGTG CAAGAATATT CTTGATGGAT TTAAGGATCT TATTCAACAA 
AATGACAAGT TAAAGAAGAC ATTAGAGGAA ATCAAGGGTA CTAAAGAATA TAAGCAGAAA 
CCAAAGAATT TGCTTCTTGA GAAAAAGGAC TGTGGTTCTT CAAAATCGCA TTTGATCGTT 
TCTCAAGAAC AAAAAGAAAT AGAGAAGGAC AGAAAACAAA TGCAGTTTGA TCATAAAGAT 
GACCATTATC ATCAAAAACA CGATACCACC ATAAGATATC CTACAGATGG AATTCCAAGA 
CTATCAGTGA CCAACATGCC CAGGACAGAA AACAAAGTGT GGTCTCTGGA CTTTCAACCA 
AGCCAAAGTC ACCAACACAA AGGAAGGTCG AAGAACAAGC CTGGTGAACC TGAAAAGAAA 
CAGGATCACC TTGATTTGAA GATAGACTCT AAAAGCATCC AAGATAAACT CGTCTGTACT 
GTGCACCAGG TGGATTCAAC TATGTTGCTT GAAGTGGTTA AATCCCTCGT TCATGATGGT 
TACACAGCCT TGATGGACCA GAGGTGTCAC AGTGCTGAAC AAATGTTCTC ACAACTGTTG 
AACATTCTCA ATCCTTCGGA ATTAAAGCAA TTGAATCTTC GTGTTATTAA TTATGTTGTA 
ATCATCTATG GACATGCCAC TTCTCTGCTT GGAATAGGAC AGCCCGAGGC GTTGACAAAG 
GCTGAAGACC AGTTTAAAAG AATAATTATA CAGTACCAGC AAGAAAGATT TGATTGTCTG 
GCATACTATG GAATTGGAAA AGTATACCTC AGACAAAACA GATTTTTAGA TGCGCTCAAT 
CAGTTTAAGA AGTCACAAAC AATGATTAAT CATAAGATTG TACCTGGAGT ATTAACTTGG 
CCCACGACAT CTCAGGTCAT TGAAGAAACA CGAACAGAGA ATTTACAGTT GTTCTTGAAG 
AACTGCATTG AGGAATGCAA GTTCCCTCCA GAACCGGATG CAATTTGTCG CTATCAGCAG 
TGCCATGGCC ACTCCAAAAT CCAAATATTT TTTACAGATC CAGACTTTAA GGGTTTTATA 
CGTATTACTT GCTGTCAGCA ATGTAGAGTC GAGTTTCATA CCAGCTGTTG GAAAAAGTTG 
AAAACTGCAA ACTACAGTGA TAAGAATGAC AAGGATTTTC TTCAAGAATT ATGTTTCACT 
CCCGACTGTA GAGGCCTTAT TTCAAAAATA GTTATTTTCA GTTCTTCTGG TTTGGTAAAA 
CGTGAATTTG AACAAAAAAT CACAAAACCC AAGGAACCAC CAAGAGCCAT TATTAAACAG 
AAATGTTCAA GTTTTAGGAA GTTAAAAAGG AAAGAAGAAA AAAAAATACG AAGAAAACGT 
GCACGGGAAG ATGCACTGAA TTCTTCTAAA CAGAAAATAG AAGAAAATCA GATGCGAAAT 
GAACTGTCTC AGTACCGTAA TCGCAGTGAT TATGCAGGTT ATACTGGTGA TCAAGTCCTG 
CAGCGTATCA TTCAAAATGC TGAGCAAATA AAGACTGCTG TTCGTGATGC CTCCAAACTG 
TTAAAGGAAT TACTTTCCTG GTTTGTAATC AGTGAAGAGG ATTACACATC ATATTGCAAA 
ACTAGCAGCA TGTCACATGC TGTGGTGGAG CAGCTCATCG GTTACTTGAT TCAGGAAGAA 
AACAGAGTGA AAACAAGGAG TTTTATCCGT GTCCTGAGTG AAATGGAAGA AGTGGATCCC 
AAATTACAGA GGTGGATGAA ACACCTTAAT GATTTGGGGT TGAGAGCTAC AGTGAACTTT 
ATCCATCAGT ATGGACAATT TTTAAAACAA CTTGACTTTT CTATTTTATC TGCACCCTGG 
AATGAGAAGT ATGGTAGTAA ATTTGACTAC GTGTCAAGTA GTTCTGAAGG CAAAGAAATT 
CTTGACTATT TTTTAAAACC ACCTCTAGAA AAGGTCCGTT GTTTTATATG GCTGTTAGAA 
GACAACAGAG AACATTTTCC ATCTCTTCAT CAAGCTTTAG ATGAATTCTT TGATAAAACG 
GACAACCCTA CCATCATATT AAAGAAGCAT GGGAATGAAG ATATATTGAC TAATGGTATC 
AAAGTTAAAA ACAAAAGCAG AAAGAAGAAC TATAAAGACT CACGGTCTGT TATAGTGTTA 
TCCAGTGGAG TTAGTACAGC ACCACAAGAA GAAAATACAT TTATAGAAGA AACTACATTA 
GATTGTACGT ATTATGAACC ATGTATTATC CCAGAATATC TTCGGAAGCA ACTAGAGGAG 
TTTGAATCCG TGTATGACAT TTCAAACAGT AACAGCTATC AAAGACTGGA TGATAATCTA 
AATCAAACCT GTGAAAGTTT ATATGACTAT TTCTCTCAGA TATTGGAAGA GCATGGACCG 
ATGGAATTAA ATAATAAGTT GCTGGTTGGG GAATATGAAC ATTTCCCTGA AGAAGCTCGA 
AAGATTGTAG AAGATGAGGG TGGTTTGAAA TCTTTTCTTC TGAAATCCCA TCGTTTCGTC 
ATGGTGGATA ATTTTATTAG CTTAAGACAT GATGTTCTTA TTAAAGAAAA TAAAAACAGA 
GACGAGACTG AAAATAATGA AGAAGGAAAT TATATTTTCT GTGATGCGCA AGAAAATTCT 
TTCCAGAACA AGATACAGTT AAATCCAGCT GCTAAGGAAT TCAAACCACT GTCTTATCCT 
GAACAACCTC ATCTATCAAC ATCTACTGAC ATATCATTAG AAAGCTGTGA GACTCCACAA 
TGTTTGCCCT ACTCTTTGCC TACTTCATGG CAATCAATGC TTAGTCAGGA TATTGATCCT 
GTCGAAAATA CGCCTTATTT TTCAAATGTT TTATTGTATC AAGATTTTCA GAATCAAAGC 
AGTTTTTTGC CTCAGACTTC CTGGGGTTAT CAGTGTGAAA GAACATCGCC AGTGCCTTCT 
CCAGTGCCTC TCCTGTCAAA TGTTGCCAAG CAACCTGGTT ATATTTATGC TGATTGTGTC 
GCTCATCAGA ATAATGATGA ATTAGCAATT TCAGACAGAA ATTATGTCAG CAACAGCTTT 
ATACCAGCTG AAATAGAAAC ATACAGAGAC CCTCAGATCC TGGTGGAGAA CTCGGATAAC 
GCATTTTATG AGGACAATTT TGCTGTTGCA AATAGTACAA GTGAAGCTGA ATGTGATACT 
CAGGTGATTA AGAAGGAAAA AGAAAGCAGA GGTATACCTG TAATGAAAAA CAATCCGCAC 
ACGAGGATGG TAGCTGTTCA GGTGAATAGT GAAGTAACTG ATAGGGAAAC AAATACTTTG 
CCTTTTCATC CATTTGAGAA GCAACAAGGA GATATTCTAC GTATGGAAAA AGAGCATCAA 
GTATTAAAAG AGCAACTTAA AGAAGCACAG GAAAAATACG AACAATTACA GAACCGGAGC 
TCAGAGGAAA TCAGTGTTTT AGAAGAGCTG ATAAAGAAGA TTGTTGAAGA GAAAGGGATA 
TTTAAGAAAG AAGTGGAGTG GCTTCATCAA TCTTTAGAAA TAAAAGTAAA AAAATGGCAA 
CAGGAGAAAA AGGAAAATCA AGAGAACTTA AAAGCAATGA GGAACACAGC CAAAAAGCAT 
ATGGATACCA ATGACAGGTA TTCAAAGACC ATTGATGAGA AGGAGAAGCA GTATAACACA 
TGTTTGAATA CTTATCTTGA GACCAGTAAT AAATTTGCTA ATGAAAAAGT AAAATTGGAA 
GAACTTATTA AAAAGAGTCA AGATGACCTC AAGGAGCATG AGAAAAGAGC TGTTACAGCA 
GAGATATCAG TACTCAAAAA CTGGAAAGAA AGCGAACTGT GCAAACTAAG TGGCATAGTT 
GCCAGAGCAG AAACAAATCT CAAGATGTTG AAGTCACTAA GCAGCAACTC AGCTTCAGCG 
CTTCCTGACT TAAAGTCACA GATTAATTAT TGGGAAGTAT TTATTTCAAA TATAAAGAAA 
CAAATGGAGA AAGTAAAGGT TGGGTACGAA GAAAAAATTG AGAAGGTGAA AAATGGTGTT 
CGAAACTGCC TCACCAAAAT AGAAGCTGCG GATCTTTCAT CTCTTCCCAG TGTCTTAAGA 
AAGCAAAGCA GACCTGCTGT CAGTGATCCA GCCCTTGTCC TGTGTTCCTC TGCATCTGCA 
CAGCCTAATT TACTTCCTGC AGTTTCGAGT TCTGAAGATC AAGGTCCAAC AACTGCTTCA 
TTTAATAGCC AGGCTGGAGT TAAGCCTGCA AATGCAAATT CTAAGGTTTG CTCTGCAAGT 
GATGGATCAG TGAAAACAAA CTCCAAAGCT CGGACAGGAT CGTATGCTGA TAAAGTTTCG 
CTAAATTCTA TGCCAAAGGT TTCAGGAAGC AATACTCAGA ATATACAGCC AAACCAAACC 
CACCAAGATG ATTCAGCTAC ACAGATGAAG CCCACTTCAA ACAACACATT TGAAAATATT 
ATTGCTCATC TACAAACTAT ATTTCCGGGC TACAGCAGTT CAGAGTTCAC GAGGTTCATA 
AAAGATGTAC AAAGAAGAAG TGGGAATACA TTAAGTTTGA GCTCCACTGA AATTCTAAGC 
AGAGTGACAG ATCTCATTCT GGACCAGCAA AATGAGGCAT CAGCTTCTGT AGGGAGACCT 
ATGATGTCTG CATCATCCGC TTCAGCGGGA CAGACGGGAA CTCAAAGTCA GGAAGCAGCT 
GCAGAAGGAA ATATTTCCAG CGCACCCAAG GCAAGGCCTG CACACAAAAG CACCTCCAGT 
AAAAACACGC TGCCATCTCA GCCAAATCTC CAGCCATGGG GGAATGCTGG AGCAACACCG 
AAATCTAAAT GGAAAAAACT AGACTATATA CCTTCCAGCG ATGATCCGTG CACCATCTGC 
CATGATGAGT TAAGCAGAGA CTCATGTGAA CTGGAGTGTG GGCATCATTT TCACAGAGAA 
TGCATTAGAA AATGGCTGAA GGAACATTCG AGTACCTGCC CTATCTGTCG CATCCATGTT 
CTGTTACCTG AAGACTTTCC TGAGCTCCCT GCACGCAACA AATATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001034.1
CDS	109..5859
Translation	MELEPWVNLCLNLSLRPWLSVLVINPAVNCSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_025145266.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145266.1

ORF Insert Sequence:

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGC AGTTTACAGA TTTTAGACTG GAATTGGAAG 
AAACTCTGTG ATTTAGAGCT TGTGGAAGAC CTTGTTGATT TGACTAAAAA AGTGGTTAAT 
GTTCCACATT TTATTGGGGG CATACTGAGA ATTGGCAGCA GGTTAGAAAA CGGTTTCTTC 
GATGTTAGGG ATGCACTTGA TTGGATCAGA TGTACAGGTG ATATCAATAT TCTGCAGAAG 
CTGGAGAAAC TTGGTGATTT TGGTTGGCTC TATCTTGAAA CTTTCTTTGG TGAATGCAAA 
CATTATATTA CTAAAGTTGC TTTGGAAGAT TGTAATCTAG TTGAAGAATT TAAAGCTGTC 
ACCTGTGAAA ACTGCAGGAA GAAGAGTGAA TCTATGAAAC AGAAAGGAAA TGAGGAGTTC 
TCCCAAGGAA AATATGATCA TGCTATAATA TCATATACTA AAGCCATAGA GTTTTGTCCT 
GCAAACCATC TTCTGTATGG AAACCGAGCT CTTTGTTTAA TTCTCACGAA GCAGTACAAG 
AGAGCCCTTG CTGATGGAAA AAGGGCCACT ATTTTGAAGC CTAATTGGCC AAAGGGTCAT 
TACCACTTCT GTAAGGCGCT GTCATTACTG GGGGAGCATG AACTGGCTTT AGAAGCTAAT 
GAGAGAGCTC AGGAACTGTG CAAGAATATT CTTGATGGAT TTAAGGATCT TATTCAACAA 
AATGACAAGT TAAAGAAGAC ATTAGAGGAA ATCAAGGGTA CTAAAGAATA TAAGCAGAAA 
CCAAAGAATT TGCTTCTTGA GAAAAAGGAC TGTGGTTCTT CAAAATCGCA TTTGATCGTT 
TCTCAAGAAC AAAAAGAAAT AGAGAAGGAC AGAAAACAAA TGCAGTTTGA TCATAAAGAT 
GACCATTATC ATCAAAAACA CGATACCACC ATAAGATATC CTACAGATGG AATTCCAAGA 
CTATCAGTGA CCAACATGCC CAGGACAGAA AACAAAGTGT GGTCTCTGGA CTTTCAACCA 
AGCCAAAGTC ACCAACACAA AGGAAGGTCG AAGAACAAGC CTGGTGAACC TGAAAAGAAA 
CAGGATCACC TTGATTTGAA GATAGACTCT AAAAGCATCC AAGATAAACT CGTCTGTACT 
GTGCACCAGG TGGATTCAAC TATGTTGCTT GAAGTGGTTA AATCCCTCGT TCATGATGGT 
TACACAGCCT TGATGGACCA GAGGTGTCAC AGTGCTGAAC AAATGTTCTC ACAACTGTTG 
AACATTCTCA ATCCTTCGGA ATTAAAGCAA TTGAATCTTC GTGTTATTAA TTATGTTGTA 
ATCATCTATG GACATGCCAC TTCTCTGCTT GGAATAGGAC AGCCCGAGGC GTTGACAAAG 
GCTGAAGACC AGTTTAAAAG AATAATTATA CAGTACCAGC AAGAAAGATT TGATTGTCTG 
GCATACTATG GAATTGGAAA AGTATACCTC AGACAAAACA GATTTTTAGA TGCGCTCAAT 
CAGTTTAAGA AGTCACAAAC AATGATTAAT CATAAGATTG TACCTGGAGT ATTAACTTGG 
CCCACGACAT CTCAGGTCAT TGAAGAAACA CGAACAGAGA ATTTACAGTT GTTCTTGAAG 
AACTGCATTG AGGAATGCAA GTTCCCTCCA GAACCGGATG CAATTTGTCG CTATCAGCAG 
TGCCATGGCC ACTCCAAAAT CCAAATATTT TTTACAGATC CAGACTTTAA GGGTTTTATA 
CGTATTACTT GCTGTCAGCA ATGTAGAGTC GAGTTTCATA CCAGCTGTTG GAAAAAGTTG 
AAAACTGCAA ACTACAGTGA TAAGAATGAC AAGGATTTTC TTCAAGAATT ATGTTTCACT 
CCCGACTGTA GAGGCCTTAT TTCAAAAATA GTTATTTTCA GTTCTTCTGG TTTGGTAAAA 
CGTGAATTTG AACAAAAAAT CACAAAACCC AAGGAACCAC CAAGAGCCAT TATTAAACAG 
AAATGTTCAA GTTTTAGGAA GTTAAAAAGG AAAGAAGAAA AAAAAATACG AAGAAAACGT 
GCACGGGAAG ATGCACTGAA TTCTTCTAAA CAGAAAATAG AAGAAAATCA GATGCGAAAT 
GAACTGTCTC AGTACCGTAA TCGCAGTGAT TATGCAGGTT ATACTGGTGA TCAAGTCCTG 
CAGCGTATCA TTCAAAATGC TGAGCAAATA AAGACTGCTG TTCGTGATGC CTCCAAACTG 
TTAAAGGAAT TACTTTCCTG GTTTGTAATC AGTGAAGAGG ATTACACATC ATATTGCAAA 
ACTAGCAGCA TGTCACATGC TGTGGTGGAG CAGCTCATCG GTTACTTGAT TCAGGAAGAA 
AACAGAGTGA AAACAAGGAG TTTTATCCGT GTCCTGAGTG AAATGGAAGA AGTGGATCCC 
AAATTACAGA GGTGGATGAA ACACCTTAAT GATTTGGGGT TGAGAGCTAC AGTGAACTTT 
ATCCATCAGT ATGGACAATT TTTAAAACAA CTTGACTTTT CTATTTTATC TGCACCCTGG 
AATGAGAAGT ATGGTAGTAA ATTTGACTAC GTGTCAAGTA GTTCTGAAGG CAAAGAAATT 
CTTGACTATT TTTTAAAACC ACCTCTAGAA AAGGTCCGTT GTTTTATATG GCTGTTAGAA 
GACAACAGAG AACATTTTCC ATCTCTTCAT CAAGCTTTAG ATGAATTCTT TGATAAAACG 
GACAACCCTA CCATCATATT AAAGAAGCAT GGGAATGAAG ATATATTGAC TAATGGTATC 
AAAGTTAAAA ACAAAAGCAG AAAGAAGAAC TATAAAGACT CACGGTCTGT TATAGTGTTA 
TCCAGTGGAG TTAGTACAGC ACCACAAGAA GAAAATACAT TTATAGAAGA AACTACATTA 
GATTGTACGT ATTATGAACC ATGTATTATC CCAGAATATC TTCGGAAGCA ACTAGAGGAG 
TTTGAATCCG TGTATGACAT TTCAAACAGT AACAGCTATC AAAGACTGGA TGATAATCTA 
AATCAAACCT GTGAAAGTTT ATATGACTAT TTCTCTCAGA TATTGGAAGA GCATGGACCG 
ATGGAATTAA ATAATAAGTT GCTGGTTGGG GAATATGAAC ATTTCCCTGA AGAAGCTCGA 
AAGATTGTAG AAGATGAGGG TGGTTTGAAA TCTTTTCTTC TGAAATCCCA TCGTTTCGTC 
ATGGTGGATA ATTTTATTAG CTTAAGACAT GATGTTCTTA TTAAAGAAAA TAAAAACAGA 
GACGAGACTG AAAATAATGA AGAAGGAAAT TATATTTTCT GTGATGCGCA AGAAAATTCT 
TTCCAGAACA AGATACAGTT AAATCCAGCT GCTAAGGAAT TCAAACCACT GTCTTATCCT 
GAACAACCTC ATCTATCAAC ATCTACTGAC ATATCATTAG AAAGCTGTGA GACTCCACAA 
TGTTTGCCCT ACTCTTTGCC TACTTCATGG CAATCAATGC TTAGTCAGGA TATTGATCCT 
GTCGAAAATA CGCCTTATTT TTCAAATGTT TTATTGTATC AAGATTTTCA GAATCAAAGC 
AGTTTTTTGC CTCAGACTTC CTGGGGTTAT CAGTGTGAAA GAACATCGCC AGTGCCTTCT 
CCAGTGCCTC TCCTGTCAAA TGTTGCCAAG CAACCTGGTT ATATTTATGC TGATTGTGTC 
GCTCATCAGA ATAATGATGA ATTAGCAATT TCAGACAGAA ATTATGTCAG CAACAGCTTT 
ATACCAGCTG AAATAGAAAC ATACAGAGAC CCTCAGATCC TGGTGGAGAA CTCGGATAAC 
GCATTTTATG AGGACAATTT TGCTGTTGCA AATAGTACAA GTGAAGCTGA ATGTGATACT 
CAGGTGATTA AGAAGGAAAA AGAAAGCAGA GGTATACCTG TAATGAAAAA CAATCCGCAC 
ACGAGGATGG TAGCTGTTCA GGTGAATAGT GAAGTAACTG ATAGGGAAAC AAATACTTTG 
CCTTTTCATC CATTTGAGAA GCAACAAGGA GATATTCTAC GTATGGAAAA AGAGCATCAA 
GTATTAAAAG AGCAACTTAA AGAAGCACAG GAAAAATACG AACAATTACA GAACCGGAGC 
TCAGAGGAAA TCAGTGTTTT AGAAGAGCTG ATAAAGAAGA TTGTTGAAGA GAAAGGGATA 
TTTAAGAAAG AAGTGGAGTG GCTTCATCAA TCTTTAGAAA TAAAAGTAAA AAAATGGCAA 
CAGGAGAAAA AGGAAAATCA AGAGAACTTA AAAGCAATGA GGAACACAGC CAAAAAGCAT 
ATGGATACCA ATGACAGGTA TTCAAAGACC ATTGATGAGA AGGAGAAGCA GTATAACACA 
TGTTTGAATA CTTATCTTGA GACCAGTAAT AAATTTGCTA ATGAAAAAGT AAAATTGGAA 
GAACTTATTA AAAAGAGTCA AGATGACCTC AAGGAGCATG AGAAAAGAGC TGTTACAGCA 
GAGATATCAG TACTCAAAAA CTGGAAAGAA AGCGAACTGT GCAAACTAAG TGGCATAGTT 
GCCAGAGCAG AAACAAATCT CAAGATGTTG AAGTCACTAA GCAGCAACTC AGCTTCAGCG 
CTTCCTGACT TAAAGTCACA GATTAATTAT TGGGAAGTAT TTATTTCAAA TATAAAGAAA 
CAAATGGAGA AAGTAAAGGT TGGGTACGAA GAAAAAATTG AGAAGGTGAA AAATGGTGTT 
CGAAACTGCC TCACCAAAAT AGAAGCTGCG GATCTTTCAT CTCTTCCCAG TGTCTTAAGA 
AAGCAAAGCA GACCTGCTGT CAGTGATCCA GCCCTTGTCC TGTGTTCCTC TGCATCTGCA 
CAGCCTAATT TACTTCCTGC AGTTTCGAGT TCTGAAGATC AAGGTCCAAC AACTGCTTCA 
TTTAATAGCC AGGCTGGAGT TAAGCCTGCA AATGCAAATT CTAAGGTTTG CTCTGCAAGT 
GATGGATCAG TGAAAACAAA CTCCAAAGCT CGGACAGGAT CGTATGCTGA TAAAGTTTCG 
CTAAATTCTA TGCCAAAGGT TTCAGGAAGC AATACTCAGA ATATACAGCC AAACCAAACC 
CACCAAGATG ATTCAGCTAC ACAGATGAAG CCCACTTCAA ACAACACATT TGAAAATATT 
ATTGCTCATC TACAAACTAT ATTTCCGGGC TACAGCAGTT CAGAGTTCAC GAGGTTCATA 
AAAGATGTAC AAAGAAGAAG TGGGAATACA TTAAGTTTGA GCTCCACTGA AATTCTAAGC 
AGAGTGACAG ATCTCATTCT GGACCAGCAA AATGAGGCAT CAGCTTCTGT AGGGAGACCT 
ATGATGTCTG CATCATCCGC TTCAGCGGGA CAGACGGGAA CTCAAAGTCA GGAAGCAGCT 
GCAGAAGGAA ATATTTCCAG CGCACCCAAG GCAAGGCCTG CACACAAAAG CACCTCCAGT 
AAAAACACGC TGCCATCTCA GCCAAATCTC CAGCCATGGG GGAATGCTGG AGCAACACCG 
AAATCTAAAT GGAAAAAACT AGACTATATA CCTTCCAGCG ATGATCCGTG CACCATCTGC 
CATGATGAGT TAAGCAGAGA CTCATGTGAA CTGGAGTGTG GGCATCATTT TCACAGAGAA 
TGCATTAGAA AATGGCTGAA GGAACATTCG AGTACCTGCC CTATCTGTCG CATCCATGTT 
CTGTTACCTG AAGACTTTCC TGAGCTCCCT GCACGCAACA AATATGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_025145271.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001039.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

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CloneID	OGa142941
Clone ID Related Accession (Same CDS sequence)	XM_025145271.1 , XM_025145273.1
Accession Version	XM_025145271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGA AAGGAAATGA GGAGTTCTCC CAAGGAAAAT ATGATCATGC TATAATATCA 
TATACTAAAG CCATAGAGTT TTGTCCTGCA AACCATCTTC TGTATGGAAA CCGAGCTCTT 
TGTTTAATTC TCACGAAGCA GTACAAGAGA GCCCTTGCTG ATGGAAAAAG GGCCACTATT 
TTGAAGCCTA ATTGGCCAAA GGGTCATTAC CACTTCTGTA AGGCGCTGTC ATTACTGGGG 
GAGCATGAAC TGGCTTTAGA AGCTAATGAG AGAGCTCAGG AACTGTGCAA GAATATTCTT 
GATGGATTTA AGGATCTTAT TCAACAAAAT GACAAGTTAA AGAAGACATT AGAGGAAATC 
AAGGGTACTA AAGAATATAA GCAGAAACCA AAGAATTTGC TTCTTGAGAA AAAGGACTGT 
GGTTCTTCAA AATCGCATTT GATCGTTTCT CAAGAACAAA AAGAAATAGA GAAGGACAGA 
AAACAAATGC AGTTTGATCA TAAAGATGAC CATTATCATC AAAAACACGA TACCACCATA 
AGATATCCTA CAGATGGAAT TCCAAGACTA TCAGTGACCA ACATGCCCAG GACAGAAAAC 
AAAGTGTGGT CTCTGGACTT TCAACCAAGC CAAAGTCACC AACACAAAGG AAGGTCGAAG 
AACAAGCCTG GTGAACCTGA AAAGAAACAG GATCACCTTG ATTTGAAGAT AGACTCTAAA 
AGCATCCAAG ATAAACTCGT CTGTACTGTG CACCAGGTGG ATTCAACTAT GTTGCTTGAA 
GTGGTTAAAT CCCTCGTTCA TGATGGTTAC ACAGCCTTGA TGGACCAGAG GTGTCACAGT 
GCTGAACAAA TGTTCTCACA ACTGTTGAAC ATTCTCAATC CTTCGGAATT AAAGCAATTG 
AATCTTCGTG TTATTAATTA TGTTGTAATC ATCTATGGAC ATGCCACTTC TCTGCTTGGA 
ATAGGACAGC CCGAGGCGTT GACAAAGGCT GAAGACCAGT TTAAAAGAAT AATTATACAG 
TACCAGCAAG AAAGATTTGA TTGTCTGGCA TACTATGGAA TTGGAAAAGT ATACCTCAGA 
CAAAACAGAT TTTTAGATGC GCTCAATCAG TTTAAGAAGT CACAAACAAT GATTAATCAT 
AAGATTGTAC CTGGAGTATT AACTTGGCCC ACGACATCTC AGGTCATTGA AGAAACACGA 
ACAGAGAATT TACAGTTGTT CTTGAAGAAC TGCATTGAGG AATGCAAGTT CCCTCCAGAA 
CCGGATGCAA TTTGTCGCTA TCAGCAGTGC CATGGCCACT CCAAAATCCA AATATTTTTT 
ACAGATCCAG ACTTTAAGGG TTTTATACGT ATTACTTGCT GTCAGCAATG TAGAGTCGAG 
TTTCATACCA GCTGTTGGAA AAAGTTGAAA ACTGCAAACT ACAGTGATAA GAATGACAAG 
GATTTTCTTC AAGAATTATG TTTCACTCCC GACTGTAGAG GCCTTATTTC AAAAATAGTT 
ATTTTCAGTT CTTCTGGTTT GGTAAAACGT GAATTTGAAC AAAAAATCAC AAAACCCAAG 
GAACCACCAA GAGCCATTAT TAAACAGAAA TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA 
GAAGAAAAAA AAATACGAAG AAAACGTGCA CGGGAAGATG CACTGAATTC TTCTAAACAG 
AAAATAGAAG AAAATCAGAT GCGAAATGAA CTGTCTCAGT ACCGTAATCG CAGTGATTAT 
GCAGGTTATA CTGGTGATCA AGTCCTGCAG CGTATCATTC AAAATGCTGA GCAAATAAAG 
ACTGCTGTTC GTGATGCCTC CAAACTGTTA AAGGAATTAC TTTCCTGGTT TGTAATCAGT 
GAAGAGGATT ACACATCATA TTGCAAAACT AGCAGCATGT CACATGCTGT GGTGGAGCAG 
CTCATCGGTT ACTTGATTCA GGAAGAAAAC AGAGTGAAAA CAAGGAGTTT TATCCGTGTC 
CTGAGTGAAA TGGAAGAAGT GGATCCCAAA TTACAGAGGT GGATGAAACA CCTTAATGAT 
TTGGGGTTGA GAGCTACAGT GAACTTTATC CATCAGTATG GACAATTTTT AAAACAACTT 
GACTTTTCTA TTTTATCTGC ACCCTGGAAT GAGAAGTATG GTAGTAAATT TGACTACGTG 
TCAAGTAGTT CTGAAGGCAA AGAAATTCTT GACTATTTTT TAAAACCACC TCTAGAAAAG 
GTCCGTTGTT TTATATGGCT GTTAGAAGAC AACAGAGAAC ATTTTCCATC TCTTCATCAA 
GCTTTAGATG AATTCTTTGA TAAAACGGAC AACCCTACCA TCATATTAAA GAAGCATGGG 
AATGAAGATA TATTGACTAA TGGTATCAAA GTTAAAAACA AAAGCAGAAA GAAGAACTAT 
AAAGACTCAC GGTCTGTTAT AGTGTTATCC AGTGGAGTTA GTACAGCACC ACAAGAAGAA 
AATACATTTA TAGAAGAAAC TACATTAGAT TGTACGTATT ATGAACCATG TATTATCCCA 
GAATATCTTC GGAAGCAACT AGAGGAGTTT GAATCCGTGT ATGACATTTC AAACAGTAAC 
AGCTATCAAA GACTGGATGA TAATCTAAAT CAAACCTGTG AAAGTTTATA TGACTATTTC 
TCTCAGATAT TGGAAGAGCA TGGACCGATG GAATTAAATA ATAAGTTGCT GGTTGGGGAA 
TATGAACATT TCCCTGAAGA AGCTCGAAAG ATTGTAGAAG ATGAGGGTGG TTTGAAATCT 
TTTCTTCTGA AATCCCATCG TTTCGTCATG GTGGATAATT TTATTAGCTT AAGACATGAT 
GTTCTTATTA AAGAAAATAA AAACAGAGAC GAGACTGAAA ATAATGAAGA AGGAAATTAT 
ATTTTCTGTG ATGCGCAAGA AAATTCTTTC CAGAACAAGA TACAGTTAAA TCCAGCTGCT 
AAGGAATTCA AACCACTGTC TTATCCTGAA CAACCTCATC TATCAACATC TACTGACATA 
TCATTAGAAA GCTGTGAGAC TCCACAATGT TTGCCCTACT CTTTGCCTAC TTCATGGCAA 
TCAATGCTTA GTCAGGATAT TGATCCTGTC GAAAATACGC CTTATTTTTC AAATGTTTTA 
TTGTATCAAG ATTTTCAGAA TCAAAGCAGT TTTTTGCCTC AGACTTCCTG GGGTTATCAG 
TGTGAAAGAA CATCGCCAGT GCCTTCTCCA GTGCCTCTCC TGTCAAATGT TGCCAAGCAA 
CCTGGTTATA TTTATGCTGA TTGTGTCGCT CATCAGAATA ATGATGAATT AGCAATTTCA 
GACAGAAATT ATGTCAGCAA CAGCTTTATA CCAGCTGAAA TAGAAACATA CAGAGACCCT 
CAGATCCTGG TGGAGAACTC GGATAACGCA TTTTATGAGG ACAATTTTGC TGTTGCAAAT 
AGTACAAGTG AAGCTGAATG TGATACTCAG GTGATTAAGA AGGAAAAAGA AAGCAGAGGT 
ATACCTGTAA TGAAAAACAA TCCGCACACG AGGATGGTAG CTGTTCAGGT GAATAGTGAA 
GTAACTGATA GGGAAACAAA TACTTTGCCT TTTCATCCAT TTGAGAAGCA ACAAGGAGAT 
ATTCTACGTA TGGAAAAAGA GCATCAAGTA TTAAAAGAGC AACTTAAAGA AGCACAGGAA 
AAATACGAAC AATTACAGAA CCGGAGCTCA GAGGAAATCA GTGTTTTAGA AGAGCTGATA 
AAGAAGATTG TTGAAGAGAA AGGGATATTT AAGAAAGAAG TGGAGTGGCT TCATCAATCT 
TTAGAAATAA AAGTAAAAAA ATGGCAACAG GAGAAAAAGG AAAATCAAGA GAACTTAAAA 
GCAATGAGGA ACACAGCCAA AAAGCATATG GATACCAATG ACAGGTATTC AAAGACCATT 
GATGAGAAGG AGAAGCAGTA TAACACATGT TTGAATACTT ATCTTGAGAC CAGTAATAAA 
TTTGCTAATG AAAAAGTAAA ATTGGAAGAA CTTATTAAAA AGAGTCAAGA TGACCTCAAG 
GAGCATGAGA AAAGAGCTGT TACAGCAGAG ATATCAGTAC TCAAAAACTG GAAAGAAAGC 
GAACTGTGCA AACTAAGTGG CATAGTTGCC AGAGCAGAAA CAAATCTCAA GATGTTGAAG 
TCACTAAGCA GCAACTCAGC TTCAGCGCTT CCTGACTTAA AGTCACAGAT TAATTATTGG 
GAAGTATTTA TTTCAAATAT AAAGAAACAA ATGGAGAAAG TAAAGGTTGG GTACGAAGAA 
AAAATTGAGA AGGTGAAAAA TGGTGTTCGA AACTGCCTCA CCAAAATAGA AGCTGCGGAT 
CTTTCATCTC TTCCCAGTGT CTTAAGAAAG CAAAGCAGAC CTGCTGTCAG TGATCCAGCC 
CTTGTCCTGT GTTCCTCTGC ATCTGCACAG CCTAATTTAC TTCCTGCAGT TTCGAGTTCT 
GAAGATCAAG GTCCAACAAC TGCTTCATTT AATAGCCAGG CTGGAGTTAA GCCTGCAAAT 
GCAAATTCTA AGGTTTGCTC TGCAAGTGAT GGATCAGTGA AAACAAACTC CAAAGCTCGG 
ACAGGATCGT ATGCTGATAA AGTTTCGCTA AATTCTATGC CAAAGGTTTC AGGAAGCAAT 
ACTCAGAATA TACAGCCAAA CCAAACCCAC CAAGATGATT CAGCTACACA GATGAAGCCC 
ACTTCAAACA ACACATTTGA AAATATTATT GCTCATCTAC AAACTATATT TCCGGGCTAC 
AGCAGTTCAG AGTTCACGAG GTTCATAAAA GATGTACAAA GAAGAAGTGG GAATACATTA 
AGTTTGAGCT CCACTGAAAT TCTAAGCAGA GTGACAGATC TCATTCTGGA CCAGCAAAAT 
GAGGCATCAG CTTCTGTAGG GAGACCTATG ATGTCTGCAT CATCCGCTTC AGCGGGACAG 
ACGGGAACTC AAAGTCAGGA AGCAGCTGCA GAAGGAAATA TTTCCAGCGC ACCCAAGGCA 
AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA AACACGCTGC CATCTCAGCC AAATCTCCAG 
CCATGGGGGA ATGCTGGAGC AACACCGAAA TCTAAATGGA AAAAACTAGA CTATATACCT 
TCCAGCGATG ATCCGTGCAC CATCTGCCAT GATGAGTTAA GCAGAGACTC ATGTGAACTG 
GAGTGTGGGC ATCATTTTCA CAGAGAATGC ATTAGAAAAT GGCTGAAGGA ACATTCGAGT 
ACCTGCCCTA TCTGTCGCAT CCATGTTCTG TTACCTGAAG ACTTTCCTGA GCTCCCTGCA 
CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001039.1
CDS	553..5850
Translation	MKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_025145271.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145271.1

ORF Insert Sequence:

ATGAAACAGA AAGGAAATGA GGAGTTCTCC CAAGGAAAAT ATGATCATGC TATAATATCA 
TATACTAAAG CCATAGAGTT TTGTCCTGCA AACCATCTTC TGTATGGAAA CCGAGCTCTT 
TGTTTAATTC TCACGAAGCA GTACAAGAGA GCCCTTGCTG ATGGAAAAAG GGCCACTATT 
TTGAAGCCTA ATTGGCCAAA GGGTCATTAC CACTTCTGTA AGGCGCTGTC ATTACTGGGG 
GAGCATGAAC TGGCTTTAGA AGCTAATGAG AGAGCTCAGG AACTGTGCAA GAATATTCTT 
GATGGATTTA AGGATCTTAT TCAACAAAAT GACAAGTTAA AGAAGACATT AGAGGAAATC 
AAGGGTACTA AAGAATATAA GCAGAAACCA AAGAATTTGC TTCTTGAGAA AAAGGACTGT 
GGTTCTTCAA AATCGCATTT GATCGTTTCT CAAGAACAAA AAGAAATAGA GAAGGACAGA 
AAACAAATGC AGTTTGATCA TAAAGATGAC CATTATCATC AAAAACACGA TACCACCATA 
AGATATCCTA CAGATGGAAT TCCAAGACTA TCAGTGACCA ACATGCCCAG GACAGAAAAC 
AAAGTGTGGT CTCTGGACTT TCAACCAAGC CAAAGTCACC AACACAAAGG AAGGTCGAAG 
AACAAGCCTG GTGAACCTGA AAAGAAACAG GATCACCTTG ATTTGAAGAT AGACTCTAAA 
AGCATCCAAG ATAAACTCGT CTGTACTGTG CACCAGGTGG ATTCAACTAT GTTGCTTGAA 
GTGGTTAAAT CCCTCGTTCA TGATGGTTAC ACAGCCTTGA TGGACCAGAG GTGTCACAGT 
GCTGAACAAA TGTTCTCACA ACTGTTGAAC ATTCTCAATC CTTCGGAATT AAAGCAATTG 
AATCTTCGTG TTATTAATTA TGTTGTAATC ATCTATGGAC ATGCCACTTC TCTGCTTGGA 
ATAGGACAGC CCGAGGCGTT GACAAAGGCT GAAGACCAGT TTAAAAGAAT AATTATACAG 
TACCAGCAAG AAAGATTTGA TTGTCTGGCA TACTATGGAA TTGGAAAAGT ATACCTCAGA 
CAAAACAGAT TTTTAGATGC GCTCAATCAG TTTAAGAAGT CACAAACAAT GATTAATCAT 
AAGATTGTAC CTGGAGTATT AACTTGGCCC ACGACATCTC AGGTCATTGA AGAAACACGA 
ACAGAGAATT TACAGTTGTT CTTGAAGAAC TGCATTGAGG AATGCAAGTT CCCTCCAGAA 
CCGGATGCAA TTTGTCGCTA TCAGCAGTGC CATGGCCACT CCAAAATCCA AATATTTTTT 
ACAGATCCAG ACTTTAAGGG TTTTATACGT ATTACTTGCT GTCAGCAATG TAGAGTCGAG 
TTTCATACCA GCTGTTGGAA AAAGTTGAAA ACTGCAAACT ACAGTGATAA GAATGACAAG 
GATTTTCTTC AAGAATTATG TTTCACTCCC GACTGTAGAG GCCTTATTTC AAAAATAGTT 
ATTTTCAGTT CTTCTGGTTT GGTAAAACGT GAATTTGAAC AAAAAATCAC AAAACCCAAG 
GAACCACCAA GAGCCATTAT TAAACAGAAA TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA 
GAAGAAAAAA AAATACGAAG AAAACGTGCA CGGGAAGATG CACTGAATTC TTCTAAACAG 
AAAATAGAAG AAAATCAGAT GCGAAATGAA CTGTCTCAGT ACCGTAATCG CAGTGATTAT 
GCAGGTTATA CTGGTGATCA AGTCCTGCAG CGTATCATTC AAAATGCTGA GCAAATAAAG 
ACTGCTGTTC GTGATGCCTC CAAACTGTTA AAGGAATTAC TTTCCTGGTT TGTAATCAGT 
GAAGAGGATT ACACATCATA TTGCAAAACT AGCAGCATGT CACATGCTGT GGTGGAGCAG 
CTCATCGGTT ACTTGATTCA GGAAGAAAAC AGAGTGAAAA CAAGGAGTTT TATCCGTGTC 
CTGAGTGAAA TGGAAGAAGT GGATCCCAAA TTACAGAGGT GGATGAAACA CCTTAATGAT 
TTGGGGTTGA GAGCTACAGT GAACTTTATC CATCAGTATG GACAATTTTT AAAACAACTT 
GACTTTTCTA TTTTATCTGC ACCCTGGAAT GAGAAGTATG GTAGTAAATT TGACTACGTG 
TCAAGTAGTT CTGAAGGCAA AGAAATTCTT GACTATTTTT TAAAACCACC TCTAGAAAAG 
GTCCGTTGTT TTATATGGCT GTTAGAAGAC AACAGAGAAC ATTTTCCATC TCTTCATCAA 
GCTTTAGATG AATTCTTTGA TAAAACGGAC AACCCTACCA TCATATTAAA GAAGCATGGG 
AATGAAGATA TATTGACTAA TGGTATCAAA GTTAAAAACA AAAGCAGAAA GAAGAACTAT 
AAAGACTCAC GGTCTGTTAT AGTGTTATCC AGTGGAGTTA GTACAGCACC ACAAGAAGAA 
AATACATTTA TAGAAGAAAC TACATTAGAT TGTACGTATT ATGAACCATG TATTATCCCA 
GAATATCTTC GGAAGCAACT AGAGGAGTTT GAATCCGTGT ATGACATTTC AAACAGTAAC 
AGCTATCAAA GACTGGATGA TAATCTAAAT CAAACCTGTG AAAGTTTATA TGACTATTTC 
TCTCAGATAT TGGAAGAGCA TGGACCGATG GAATTAAATA ATAAGTTGCT GGTTGGGGAA 
TATGAACATT TCCCTGAAGA AGCTCGAAAG ATTGTAGAAG ATGAGGGTGG TTTGAAATCT 
TTTCTTCTGA AATCCCATCG TTTCGTCATG GTGGATAATT TTATTAGCTT AAGACATGAT 
GTTCTTATTA AAGAAAATAA AAACAGAGAC GAGACTGAAA ATAATGAAGA AGGAAATTAT 
ATTTTCTGTG ATGCGCAAGA AAATTCTTTC CAGAACAAGA TACAGTTAAA TCCAGCTGCT 
AAGGAATTCA AACCACTGTC TTATCCTGAA CAACCTCATC TATCAACATC TACTGACATA 
TCATTAGAAA GCTGTGAGAC TCCACAATGT TTGCCCTACT CTTTGCCTAC TTCATGGCAA 
TCAATGCTTA GTCAGGATAT TGATCCTGTC GAAAATACGC CTTATTTTTC AAATGTTTTA 
TTGTATCAAG ATTTTCAGAA TCAAAGCAGT TTTTTGCCTC AGACTTCCTG GGGTTATCAG 
TGTGAAAGAA CATCGCCAGT GCCTTCTCCA GTGCCTCTCC TGTCAAATGT TGCCAAGCAA 
CCTGGTTATA TTTATGCTGA TTGTGTCGCT CATCAGAATA ATGATGAATT AGCAATTTCA 
GACAGAAATT ATGTCAGCAA CAGCTTTATA CCAGCTGAAA TAGAAACATA CAGAGACCCT 
CAGATCCTGG TGGAGAACTC GGATAACGCA TTTTATGAGG ACAATTTTGC TGTTGCAAAT 
AGTACAAGTG AAGCTGAATG TGATACTCAG GTGATTAAGA AGGAAAAAGA AAGCAGAGGT 
ATACCTGTAA TGAAAAACAA TCCGCACACG AGGATGGTAG CTGTTCAGGT GAATAGTGAA 
GTAACTGATA GGGAAACAAA TACTTTGCCT TTTCATCCAT TTGAGAAGCA ACAAGGAGAT 
ATTCTACGTA TGGAAAAAGA GCATCAAGTA TTAAAAGAGC AACTTAAAGA AGCACAGGAA 
AAATACGAAC AATTACAGAA CCGGAGCTCA GAGGAAATCA GTGTTTTAGA AGAGCTGATA 
AAGAAGATTG TTGAAGAGAA AGGGATATTT AAGAAAGAAG TGGAGTGGCT TCATCAATCT 
TTAGAAATAA AAGTAAAAAA ATGGCAACAG GAGAAAAAGG AAAATCAAGA GAACTTAAAA 
GCAATGAGGA ACACAGCCAA AAAGCATATG GATACCAATG ACAGGTATTC AAAGACCATT 
GATGAGAAGG AGAAGCAGTA TAACACATGT TTGAATACTT ATCTTGAGAC CAGTAATAAA 
TTTGCTAATG AAAAAGTAAA ATTGGAAGAA CTTATTAAAA AGAGTCAAGA TGACCTCAAG 
GAGCATGAGA AAAGAGCTGT TACAGCAGAG ATATCAGTAC TCAAAAACTG GAAAGAAAGC 
GAACTGTGCA AACTAAGTGG CATAGTTGCC AGAGCAGAAA CAAATCTCAA GATGTTGAAG 
TCACTAAGCA GCAACTCAGC TTCAGCGCTT CCTGACTTAA AGTCACAGAT TAATTATTGG 
GAAGTATTTA TTTCAAATAT AAAGAAACAA ATGGAGAAAG TAAAGGTTGG GTACGAAGAA 
AAAATTGAGA AGGTGAAAAA TGGTGTTCGA AACTGCCTCA CCAAAATAGA AGCTGCGGAT 
CTTTCATCTC TTCCCAGTGT CTTAAGAAAG CAAAGCAGAC CTGCTGTCAG TGATCCAGCC 
CTTGTCCTGT GTTCCTCTGC ATCTGCACAG CCTAATTTAC TTCCTGCAGT TTCGAGTTCT 
GAAGATCAAG GTCCAACAAC TGCTTCATTT AATAGCCAGG CTGGAGTTAA GCCTGCAAAT 
GCAAATTCTA AGGTTTGCTC TGCAAGTGAT GGATCAGTGA AAACAAACTC CAAAGCTCGG 
ACAGGATCGT ATGCTGATAA AGTTTCGCTA AATTCTATGC CAAAGGTTTC AGGAAGCAAT 
ACTCAGAATA TACAGCCAAA CCAAACCCAC CAAGATGATT CAGCTACACA GATGAAGCCC 
ACTTCAAACA ACACATTTGA AAATATTATT GCTCATCTAC AAACTATATT TCCGGGCTAC 
AGCAGTTCAG AGTTCACGAG GTTCATAAAA GATGTACAAA GAAGAAGTGG GAATACATTA 
AGTTTGAGCT CCACTGAAAT TCTAAGCAGA GTGACAGATC TCATTCTGGA CCAGCAAAAT 
GAGGCATCAG CTTCTGTAGG GAGACCTATG ATGTCTGCAT CATCCGCTTC AGCGGGACAG 
ACGGGAACTC AAAGTCAGGA AGCAGCTGCA GAAGGAAATA TTTCCAGCGC ACCCAAGGCA 
AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA AACACGCTGC CATCTCAGCC AAATCTCCAG 
CCATGGGGGA ATGCTGGAGC AACACCGAAA TCTAAATGGA AAAAACTAGA CTATATACCT 
TCCAGCGATG ATCCGTGCAC CATCTGCCAT GATGAGTTAA GCAGAGACTC ATGTGAACTG 
GAGTGTGGGC ATCATTTTCA CAGAGAATGC ATTAGAAAAT GGCTGAAGGA ACATTCGAGT 
ACCTGCCCTA TCTGTCGCAT CCATGTTCTG TTACCTGAAG ACTTTCCTGA GCTCCCTGCA 
CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_025145273.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001041.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

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CloneID	OGa142941
Clone ID Related Accession (Same CDS sequence)	XM_025145271.1 , XM_025145273.1
Accession Version	XM_025145273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGA AAGGAAATGA GGAGTTCTCC CAAGGAAAAT ATGATCATGC TATAATATCA 
TATACTAAAG CCATAGAGTT TTGTCCTGCA AACCATCTTC TGTATGGAAA CCGAGCTCTT 
TGTTTAATTC TCACGAAGCA GTACAAGAGA GCCCTTGCTG ATGGAAAAAG GGCCACTATT 
TTGAAGCCTA ATTGGCCAAA GGGTCATTAC CACTTCTGTA AGGCGCTGTC ATTACTGGGG 
GAGCATGAAC TGGCTTTAGA AGCTAATGAG AGAGCTCAGG AACTGTGCAA GAATATTCTT 
GATGGATTTA AGGATCTTAT TCAACAAAAT GACAAGTTAA AGAAGACATT AGAGGAAATC 
AAGGGTACTA AAGAATATAA GCAGAAACCA AAGAATTTGC TTCTTGAGAA AAAGGACTGT 
GGTTCTTCAA AATCGCATTT GATCGTTTCT CAAGAACAAA AAGAAATAGA GAAGGACAGA 
AAACAAATGC AGTTTGATCA TAAAGATGAC CATTATCATC AAAAACACGA TACCACCATA 
AGATATCCTA CAGATGGAAT TCCAAGACTA TCAGTGACCA ACATGCCCAG GACAGAAAAC 
AAAGTGTGGT CTCTGGACTT TCAACCAAGC CAAAGTCACC AACACAAAGG AAGGTCGAAG 
AACAAGCCTG GTGAACCTGA AAAGAAACAG GATCACCTTG ATTTGAAGAT AGACTCTAAA 
AGCATCCAAG ATAAACTCGT CTGTACTGTG CACCAGGTGG ATTCAACTAT GTTGCTTGAA 
GTGGTTAAAT CCCTCGTTCA TGATGGTTAC ACAGCCTTGA TGGACCAGAG GTGTCACAGT 
GCTGAACAAA TGTTCTCACA ACTGTTGAAC ATTCTCAATC CTTCGGAATT AAAGCAATTG 
AATCTTCGTG TTATTAATTA TGTTGTAATC ATCTATGGAC ATGCCACTTC TCTGCTTGGA 
ATAGGACAGC CCGAGGCGTT GACAAAGGCT GAAGACCAGT TTAAAAGAAT AATTATACAG 
TACCAGCAAG AAAGATTTGA TTGTCTGGCA TACTATGGAA TTGGAAAAGT ATACCTCAGA 
CAAAACAGAT TTTTAGATGC GCTCAATCAG TTTAAGAAGT CACAAACAAT GATTAATCAT 
AAGATTGTAC CTGGAGTATT AACTTGGCCC ACGACATCTC AGGTCATTGA AGAAACACGA 
ACAGAGAATT TACAGTTGTT CTTGAAGAAC TGCATTGAGG AATGCAAGTT CCCTCCAGAA 
CCGGATGCAA TTTGTCGCTA TCAGCAGTGC CATGGCCACT CCAAAATCCA AATATTTTTT 
ACAGATCCAG ACTTTAAGGG TTTTATACGT ATTACTTGCT GTCAGCAATG TAGAGTCGAG 
TTTCATACCA GCTGTTGGAA AAAGTTGAAA ACTGCAAACT ACAGTGATAA GAATGACAAG 
GATTTTCTTC AAGAATTATG TTTCACTCCC GACTGTAGAG GCCTTATTTC AAAAATAGTT 
ATTTTCAGTT CTTCTGGTTT GGTAAAACGT GAATTTGAAC AAAAAATCAC AAAACCCAAG 
GAACCACCAA GAGCCATTAT TAAACAGAAA TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA 
GAAGAAAAAA AAATACGAAG AAAACGTGCA CGGGAAGATG CACTGAATTC TTCTAAACAG 
AAAATAGAAG AAAATCAGAT GCGAAATGAA CTGTCTCAGT ACCGTAATCG CAGTGATTAT 
GCAGGTTATA CTGGTGATCA AGTCCTGCAG CGTATCATTC AAAATGCTGA GCAAATAAAG 
ACTGCTGTTC GTGATGCCTC CAAACTGTTA AAGGAATTAC TTTCCTGGTT TGTAATCAGT 
GAAGAGGATT ACACATCATA TTGCAAAACT AGCAGCATGT CACATGCTGT GGTGGAGCAG 
CTCATCGGTT ACTTGATTCA GGAAGAAAAC AGAGTGAAAA CAAGGAGTTT TATCCGTGTC 
CTGAGTGAAA TGGAAGAAGT GGATCCCAAA TTACAGAGGT GGATGAAACA CCTTAATGAT 
TTGGGGTTGA GAGCTACAGT GAACTTTATC CATCAGTATG GACAATTTTT AAAACAACTT 
GACTTTTCTA TTTTATCTGC ACCCTGGAAT GAGAAGTATG GTAGTAAATT TGACTACGTG 
TCAAGTAGTT CTGAAGGCAA AGAAATTCTT GACTATTTTT TAAAACCACC TCTAGAAAAG 
GTCCGTTGTT TTATATGGCT GTTAGAAGAC AACAGAGAAC ATTTTCCATC TCTTCATCAA 
GCTTTAGATG AATTCTTTGA TAAAACGGAC AACCCTACCA TCATATTAAA GAAGCATGGG 
AATGAAGATA TATTGACTAA TGGTATCAAA GTTAAAAACA AAAGCAGAAA GAAGAACTAT 
AAAGACTCAC GGTCTGTTAT AGTGTTATCC AGTGGAGTTA GTACAGCACC ACAAGAAGAA 
AATACATTTA TAGAAGAAAC TACATTAGAT TGTACGTATT ATGAACCATG TATTATCCCA 
GAATATCTTC GGAAGCAACT AGAGGAGTTT GAATCCGTGT ATGACATTTC AAACAGTAAC 
AGCTATCAAA GACTGGATGA TAATCTAAAT CAAACCTGTG AAAGTTTATA TGACTATTTC 
TCTCAGATAT TGGAAGAGCA TGGACCGATG GAATTAAATA ATAAGTTGCT GGTTGGGGAA 
TATGAACATT TCCCTGAAGA AGCTCGAAAG ATTGTAGAAG ATGAGGGTGG TTTGAAATCT 
TTTCTTCTGA AATCCCATCG TTTCGTCATG GTGGATAATT TTATTAGCTT AAGACATGAT 
GTTCTTATTA AAGAAAATAA AAACAGAGAC GAGACTGAAA ATAATGAAGA AGGAAATTAT 
ATTTTCTGTG ATGCGCAAGA AAATTCTTTC CAGAACAAGA TACAGTTAAA TCCAGCTGCT 
AAGGAATTCA AACCACTGTC TTATCCTGAA CAACCTCATC TATCAACATC TACTGACATA 
TCATTAGAAA GCTGTGAGAC TCCACAATGT TTGCCCTACT CTTTGCCTAC TTCATGGCAA 
TCAATGCTTA GTCAGGATAT TGATCCTGTC GAAAATACGC CTTATTTTTC AAATGTTTTA 
TTGTATCAAG ATTTTCAGAA TCAAAGCAGT TTTTTGCCTC AGACTTCCTG GGGTTATCAG 
TGTGAAAGAA CATCGCCAGT GCCTTCTCCA GTGCCTCTCC TGTCAAATGT TGCCAAGCAA 
CCTGGTTATA TTTATGCTGA TTGTGTCGCT CATCAGAATA ATGATGAATT AGCAATTTCA 
GACAGAAATT ATGTCAGCAA CAGCTTTATA CCAGCTGAAA TAGAAACATA CAGAGACCCT 
CAGATCCTGG TGGAGAACTC GGATAACGCA TTTTATGAGG ACAATTTTGC TGTTGCAAAT 
AGTACAAGTG AAGCTGAATG TGATACTCAG GTGATTAAGA AGGAAAAAGA AAGCAGAGGT 
ATACCTGTAA TGAAAAACAA TCCGCACACG AGGATGGTAG CTGTTCAGGT GAATAGTGAA 
GTAACTGATA GGGAAACAAA TACTTTGCCT TTTCATCCAT TTGAGAAGCA ACAAGGAGAT 
ATTCTACGTA TGGAAAAAGA GCATCAAGTA TTAAAAGAGC AACTTAAAGA AGCACAGGAA 
AAATACGAAC AATTACAGAA CCGGAGCTCA GAGGAAATCA GTGTTTTAGA AGAGCTGATA 
AAGAAGATTG TTGAAGAGAA AGGGATATTT AAGAAAGAAG TGGAGTGGCT TCATCAATCT 
TTAGAAATAA AAGTAAAAAA ATGGCAACAG GAGAAAAAGG AAAATCAAGA GAACTTAAAA 
GCAATGAGGA ACACAGCCAA AAAGCATATG GATACCAATG ACAGGTATTC AAAGACCATT 
GATGAGAAGG AGAAGCAGTA TAACACATGT TTGAATACTT ATCTTGAGAC CAGTAATAAA 
TTTGCTAATG AAAAAGTAAA ATTGGAAGAA CTTATTAAAA AGAGTCAAGA TGACCTCAAG 
GAGCATGAGA AAAGAGCTGT TACAGCAGAG ATATCAGTAC TCAAAAACTG GAAAGAAAGC 
GAACTGTGCA AACTAAGTGG CATAGTTGCC AGAGCAGAAA CAAATCTCAA GATGTTGAAG 
TCACTAAGCA GCAACTCAGC TTCAGCGCTT CCTGACTTAA AGTCACAGAT TAATTATTGG 
GAAGTATTTA TTTCAAATAT AAAGAAACAA ATGGAGAAAG TAAAGGTTGG GTACGAAGAA 
AAAATTGAGA AGGTGAAAAA TGGTGTTCGA AACTGCCTCA CCAAAATAGA AGCTGCGGAT 
CTTTCATCTC TTCCCAGTGT CTTAAGAAAG CAAAGCAGAC CTGCTGTCAG TGATCCAGCC 
CTTGTCCTGT GTTCCTCTGC ATCTGCACAG CCTAATTTAC TTCCTGCAGT TTCGAGTTCT 
GAAGATCAAG GTCCAACAAC TGCTTCATTT AATAGCCAGG CTGGAGTTAA GCCTGCAAAT 
GCAAATTCTA AGGTTTGCTC TGCAAGTGAT GGATCAGTGA AAACAAACTC CAAAGCTCGG 
ACAGGATCGT ATGCTGATAA AGTTTCGCTA AATTCTATGC CAAAGGTTTC AGGAAGCAAT 
ACTCAGAATA TACAGCCAAA CCAAACCCAC CAAGATGATT CAGCTACACA GATGAAGCCC 
ACTTCAAACA ACACATTTGA AAATATTATT GCTCATCTAC AAACTATATT TCCGGGCTAC 
AGCAGTTCAG AGTTCACGAG GTTCATAAAA GATGTACAAA GAAGAAGTGG GAATACATTA 
AGTTTGAGCT CCACTGAAAT TCTAAGCAGA GTGACAGATC TCATTCTGGA CCAGCAAAAT 
GAGGCATCAG CTTCTGTAGG GAGACCTATG ATGTCTGCAT CATCCGCTTC AGCGGGACAG 
ACGGGAACTC AAAGTCAGGA AGCAGCTGCA GAAGGAAATA TTTCCAGCGC ACCCAAGGCA 
AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA AACACGCTGC CATCTCAGCC AAATCTCCAG 
CCATGGGGGA ATGCTGGAGC AACACCGAAA TCTAAATGGA AAAAACTAGA CTATATACCT 
TCCAGCGATG ATCCGTGCAC CATCTGCCAT GATGAGTTAA GCAGAGACTC ATGTGAACTG 
GAGTGTGGGC ATCATTTTCA CAGAGAATGC ATTAGAAAAT GGCTGAAGGA ACATTCGAGT 
ACCTGCCCTA TCTGTCGCAT CCATGTTCTG TTACCTGAAG ACTTTCCTGA GCTCCCTGCA 
CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001041.1
CDS	571..5868
Translation	MKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_025145273.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145273.1

ORF Insert Sequence:

ATGAAACAGA AAGGAAATGA GGAGTTCTCC CAAGGAAAAT ATGATCATGC TATAATATCA 
TATACTAAAG CCATAGAGTT TTGTCCTGCA AACCATCTTC TGTATGGAAA CCGAGCTCTT 
TGTTTAATTC TCACGAAGCA GTACAAGAGA GCCCTTGCTG ATGGAAAAAG GGCCACTATT 
TTGAAGCCTA ATTGGCCAAA GGGTCATTAC CACTTCTGTA AGGCGCTGTC ATTACTGGGG 
GAGCATGAAC TGGCTTTAGA AGCTAATGAG AGAGCTCAGG AACTGTGCAA GAATATTCTT 
GATGGATTTA AGGATCTTAT TCAACAAAAT GACAAGTTAA AGAAGACATT AGAGGAAATC 
AAGGGTACTA AAGAATATAA GCAGAAACCA AAGAATTTGC TTCTTGAGAA AAAGGACTGT 
GGTTCTTCAA AATCGCATTT GATCGTTTCT CAAGAACAAA AAGAAATAGA GAAGGACAGA 
AAACAAATGC AGTTTGATCA TAAAGATGAC CATTATCATC AAAAACACGA TACCACCATA 
AGATATCCTA CAGATGGAAT TCCAAGACTA TCAGTGACCA ACATGCCCAG GACAGAAAAC 
AAAGTGTGGT CTCTGGACTT TCAACCAAGC CAAAGTCACC AACACAAAGG AAGGTCGAAG 
AACAAGCCTG GTGAACCTGA AAAGAAACAG GATCACCTTG ATTTGAAGAT AGACTCTAAA 
AGCATCCAAG ATAAACTCGT CTGTACTGTG CACCAGGTGG ATTCAACTAT GTTGCTTGAA 
GTGGTTAAAT CCCTCGTTCA TGATGGTTAC ACAGCCTTGA TGGACCAGAG GTGTCACAGT 
GCTGAACAAA TGTTCTCACA ACTGTTGAAC ATTCTCAATC CTTCGGAATT AAAGCAATTG 
AATCTTCGTG TTATTAATTA TGTTGTAATC ATCTATGGAC ATGCCACTTC TCTGCTTGGA 
ATAGGACAGC CCGAGGCGTT GACAAAGGCT GAAGACCAGT TTAAAAGAAT AATTATACAG 
TACCAGCAAG AAAGATTTGA TTGTCTGGCA TACTATGGAA TTGGAAAAGT ATACCTCAGA 
CAAAACAGAT TTTTAGATGC GCTCAATCAG TTTAAGAAGT CACAAACAAT GATTAATCAT 
AAGATTGTAC CTGGAGTATT AACTTGGCCC ACGACATCTC AGGTCATTGA AGAAACACGA 
ACAGAGAATT TACAGTTGTT CTTGAAGAAC TGCATTGAGG AATGCAAGTT CCCTCCAGAA 
CCGGATGCAA TTTGTCGCTA TCAGCAGTGC CATGGCCACT CCAAAATCCA AATATTTTTT 
ACAGATCCAG ACTTTAAGGG TTTTATACGT ATTACTTGCT GTCAGCAATG TAGAGTCGAG 
TTTCATACCA GCTGTTGGAA AAAGTTGAAA ACTGCAAACT ACAGTGATAA GAATGACAAG 
GATTTTCTTC AAGAATTATG TTTCACTCCC GACTGTAGAG GCCTTATTTC AAAAATAGTT 
ATTTTCAGTT CTTCTGGTTT GGTAAAACGT GAATTTGAAC AAAAAATCAC AAAACCCAAG 
GAACCACCAA GAGCCATTAT TAAACAGAAA TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA 
GAAGAAAAAA AAATACGAAG AAAACGTGCA CGGGAAGATG CACTGAATTC TTCTAAACAG 
AAAATAGAAG AAAATCAGAT GCGAAATGAA CTGTCTCAGT ACCGTAATCG CAGTGATTAT 
GCAGGTTATA CTGGTGATCA AGTCCTGCAG CGTATCATTC AAAATGCTGA GCAAATAAAG 
ACTGCTGTTC GTGATGCCTC CAAACTGTTA AAGGAATTAC TTTCCTGGTT TGTAATCAGT 
GAAGAGGATT ACACATCATA TTGCAAAACT AGCAGCATGT CACATGCTGT GGTGGAGCAG 
CTCATCGGTT ACTTGATTCA GGAAGAAAAC AGAGTGAAAA CAAGGAGTTT TATCCGTGTC 
CTGAGTGAAA TGGAAGAAGT GGATCCCAAA TTACAGAGGT GGATGAAACA CCTTAATGAT 
TTGGGGTTGA GAGCTACAGT GAACTTTATC CATCAGTATG GACAATTTTT AAAACAACTT 
GACTTTTCTA TTTTATCTGC ACCCTGGAAT GAGAAGTATG GTAGTAAATT TGACTACGTG 
TCAAGTAGTT CTGAAGGCAA AGAAATTCTT GACTATTTTT TAAAACCACC TCTAGAAAAG 
GTCCGTTGTT TTATATGGCT GTTAGAAGAC AACAGAGAAC ATTTTCCATC TCTTCATCAA 
GCTTTAGATG AATTCTTTGA TAAAACGGAC AACCCTACCA TCATATTAAA GAAGCATGGG 
AATGAAGATA TATTGACTAA TGGTATCAAA GTTAAAAACA AAAGCAGAAA GAAGAACTAT 
AAAGACTCAC GGTCTGTTAT AGTGTTATCC AGTGGAGTTA GTACAGCACC ACAAGAAGAA 
AATACATTTA TAGAAGAAAC TACATTAGAT TGTACGTATT ATGAACCATG TATTATCCCA 
GAATATCTTC GGAAGCAACT AGAGGAGTTT GAATCCGTGT ATGACATTTC AAACAGTAAC 
AGCTATCAAA GACTGGATGA TAATCTAAAT CAAACCTGTG AAAGTTTATA TGACTATTTC 
TCTCAGATAT TGGAAGAGCA TGGACCGATG GAATTAAATA ATAAGTTGCT GGTTGGGGAA 
TATGAACATT TCCCTGAAGA AGCTCGAAAG ATTGTAGAAG ATGAGGGTGG TTTGAAATCT 
TTTCTTCTGA AATCCCATCG TTTCGTCATG GTGGATAATT TTATTAGCTT AAGACATGAT 
GTTCTTATTA AAGAAAATAA AAACAGAGAC GAGACTGAAA ATAATGAAGA AGGAAATTAT 
ATTTTCTGTG ATGCGCAAGA AAATTCTTTC CAGAACAAGA TACAGTTAAA TCCAGCTGCT 
AAGGAATTCA AACCACTGTC TTATCCTGAA CAACCTCATC TATCAACATC TACTGACATA 
TCATTAGAAA GCTGTGAGAC TCCACAATGT TTGCCCTACT CTTTGCCTAC TTCATGGCAA 
TCAATGCTTA GTCAGGATAT TGATCCTGTC GAAAATACGC CTTATTTTTC AAATGTTTTA 
TTGTATCAAG ATTTTCAGAA TCAAAGCAGT TTTTTGCCTC AGACTTCCTG GGGTTATCAG 
TGTGAAAGAA CATCGCCAGT GCCTTCTCCA GTGCCTCTCC TGTCAAATGT TGCCAAGCAA 
CCTGGTTATA TTTATGCTGA TTGTGTCGCT CATCAGAATA ATGATGAATT AGCAATTTCA 
GACAGAAATT ATGTCAGCAA CAGCTTTATA CCAGCTGAAA TAGAAACATA CAGAGACCCT 
CAGATCCTGG TGGAGAACTC GGATAACGCA TTTTATGAGG ACAATTTTGC TGTTGCAAAT 
AGTACAAGTG AAGCTGAATG TGATACTCAG GTGATTAAGA AGGAAAAAGA AAGCAGAGGT 
ATACCTGTAA TGAAAAACAA TCCGCACACG AGGATGGTAG CTGTTCAGGT GAATAGTGAA 
GTAACTGATA GGGAAACAAA TACTTTGCCT TTTCATCCAT TTGAGAAGCA ACAAGGAGAT 
ATTCTACGTA TGGAAAAAGA GCATCAAGTA TTAAAAGAGC AACTTAAAGA AGCACAGGAA 
AAATACGAAC AATTACAGAA CCGGAGCTCA GAGGAAATCA GTGTTTTAGA AGAGCTGATA 
AAGAAGATTG TTGAAGAGAA AGGGATATTT AAGAAAGAAG TGGAGTGGCT TCATCAATCT 
TTAGAAATAA AAGTAAAAAA ATGGCAACAG GAGAAAAAGG AAAATCAAGA GAACTTAAAA 
GCAATGAGGA ACACAGCCAA AAAGCATATG GATACCAATG ACAGGTATTC AAAGACCATT 
GATGAGAAGG AGAAGCAGTA TAACACATGT TTGAATACTT ATCTTGAGAC CAGTAATAAA 
TTTGCTAATG AAAAAGTAAA ATTGGAAGAA CTTATTAAAA AGAGTCAAGA TGACCTCAAG 
GAGCATGAGA AAAGAGCTGT TACAGCAGAG ATATCAGTAC TCAAAAACTG GAAAGAAAGC 
GAACTGTGCA AACTAAGTGG CATAGTTGCC AGAGCAGAAA CAAATCTCAA GATGTTGAAG 
TCACTAAGCA GCAACTCAGC TTCAGCGCTT CCTGACTTAA AGTCACAGAT TAATTATTGG 
GAAGTATTTA TTTCAAATAT AAAGAAACAA ATGGAGAAAG TAAAGGTTGG GTACGAAGAA 
AAAATTGAGA AGGTGAAAAA TGGTGTTCGA AACTGCCTCA CCAAAATAGA AGCTGCGGAT 
CTTTCATCTC TTCCCAGTGT CTTAAGAAAG CAAAGCAGAC CTGCTGTCAG TGATCCAGCC 
CTTGTCCTGT GTTCCTCTGC ATCTGCACAG CCTAATTTAC TTCCTGCAGT TTCGAGTTCT 
GAAGATCAAG GTCCAACAAC TGCTTCATTT AATAGCCAGG CTGGAGTTAA GCCTGCAAAT 
GCAAATTCTA AGGTTTGCTC TGCAAGTGAT GGATCAGTGA AAACAAACTC CAAAGCTCGG 
ACAGGATCGT ATGCTGATAA AGTTTCGCTA AATTCTATGC CAAAGGTTTC AGGAAGCAAT 
ACTCAGAATA TACAGCCAAA CCAAACCCAC CAAGATGATT CAGCTACACA GATGAAGCCC 
ACTTCAAACA ACACATTTGA AAATATTATT GCTCATCTAC AAACTATATT TCCGGGCTAC 
AGCAGTTCAG AGTTCACGAG GTTCATAAAA GATGTACAAA GAAGAAGTGG GAATACATTA 
AGTTTGAGCT CCACTGAAAT TCTAAGCAGA GTGACAGATC TCATTCTGGA CCAGCAAAAT 
GAGGCATCAG CTTCTGTAGG GAGACCTATG ATGTCTGCAT CATCCGCTTC AGCGGGACAG 
ACGGGAACTC AAAGTCAGGA AGCAGCTGCA GAAGGAAATA TTTCCAGCGC ACCCAAGGCA 
AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA AACACGCTGC CATCTCAGCC AAATCTCCAG 
CCATGGGGGA ATGCTGGAGC AACACCGAAA TCTAAATGGA AAAAACTAGA CTATATACCT 
TCCAGCGATG ATCCGTGCAC CATCTGCCAT GATGAGTTAA GCAGAGACTC ATGTGAACTG 
GAGTGTGGGC ATCATTTTCA CAGAGAATGC ATTAGAAAAT GGCTGAAGGA ACATTCGAGT 
ACCTGCCCTA TCTGTCGCAT CCATGTTCTG TTACCTGAAG ACTTTCCTGA GCTCCCTGCA 
CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301408.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156894.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa30356
Clone ID Related Accession (Same CDS sequence)	XM_015301408.2 , XM_015301408.1
Accession Version	XM_015301408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGTT TACAGATTTT AGACTGGAAT 
TGGAAGAAAC TCTGTGATTT AGAGCTTGTG GAAGACCTTG TTGATTTGAC TAAAAAAGTG 
GTTAATGTTC CACATTTTAT TGGGGGCATA CTGAGAATTG GCAGCAGGTT AGAAAACGGT 
TTCTTCGATG TTAGGGATGC ACTTGATTGG ATCAGATGTA CAGGTGATAT CAATATTCTG 
CAGAAGCTGG AGAAACTTGG TGATTTTGGT TGGCTCTATC TTGAAACTTT CTTTGGTGAA 
TGCAAACATT ATATTACTAA AGTTGCTTTG GAAGATTGTA ATCTAGTTGA AGAATTTAAA 
GCTGTCACCT GTGAAAACTG CAGGAAGAAG AGTGAATCTA TGAAACAGAA AGGAAATGAG 
GAGTTCTCCC AAGGAAAATA TGATCATGCT ATAATATCAT ATACTAAAGC CATAGAGTTT 
TGTCCTGCAA ACCATCTTCT GTATGGAAAC CGAGCTCTTT GTTTAATTCT CACGAAGCAG 
TACAAGAGAG CCCTTGCTGA TGGAAAAAGG GCCACTATTT TGAAGCCTAA TTGGCCAAAG 
GGTCATTACC ACTTCTGTAA GGCGCTGTCA TTACTGGGGG AGCATGAACT GGCTTTAGAA 
GCTAATGAGA GAGCTCAGGA ACTGTGCAAG AATATTCTTG ATGGATTTAA GGATCTTATT 
CAACAAAATG ACAAGTTAAA GAAGACATTA GAGGAAATCA AGGGTACTAA AGAATATAAG 
CAGAAACCAA AGAATTTGCT TCTTGAGAAA AAGGACTGTG GTTCTTCAAA ATCGCATTTG 
ATCGTTTCTC AAGAACAAAA AGAAATAGAG AAGGACAGAA AACAAATGCA GTTTGATCAT 
AAAGATGACC ATTATCATCA AAAACACGAT ACCACCATAA GATATCCTAC AGATGGAATT 
CCAAGACTAT CAGTGACCAA CATGCCCAGG ACAGAAAACA AAGTGTGGTC TCTGGACTTT 
CAACCAAGCC AAAGTCACCA ACACAAAGGA AGGTCGAAGA ACAAGCCTGG TGAACCTGAA 
AAGAAACAGG ATCACCTTGA TTTGAAGATA GACTCTAAAA GCATCCAAGA TAAACTCGTC 
TGTACTGTGC ACCAGGTGGA TTCAACTATG TTGCTTGAAG TGGTTAAATC CCTCGTTCAT 
GATGGTTACA CAGCCTTGAT GGACCAGAGG TGTCACAGTG CTGAACAAAT GTTCTCACAA 
CTGTTGAACA TTCTCAATCC TTCGGAATTA AAGCAATTGA ATCTTCGTGT TATTAATTAT 
GTTGTAATCA TCTATGGACA TGCCACTTCT CTGCTTGGAA TAGGACAGCC CGAGGCGTTG 
ACAAAGGCTG AAGACCAGTT TAAAAGAATA ATTATACAGT ACCAGCAAGA AAGATTTGAT 
TGTCTGGCAT ACTATGGAAT TGGAAAAGTA TACCTCAGAC AAAACAGATT TTTAGATGCG 
CTCAATCAGT TTAAGAAGTC ACAAACAATG ATTAATCATA AGATTGTACC TGGAGTATTA 
ACTTGGCCCA CGACATCTCA GGTCATTGAA GAAACACGAA CAGAGAATTT ACAGTTGTTC 
TTGAAGAACT GCATTGAGGA ATGCAAGTTC CCTCCAGAAC CGGATGCAAT TTGTCGCTAT 
CAGCAGTGCC ATGGCCACTC CAAAATCCAA ATATTTTTTA CAGATCCAGA CTTTAAGGGT 
TTTATACGTA TTACTTGCTG TCAGCAATGT AGAGTCGAGT TTCATACCAG CTGTTGGAAA 
AAGTTGAAAA CTGCAAACTA CAGTGATAAG AATGACAAGG ATTTTCTTCA AGAATTATGT 
TTCACTCCCG ACTGTAGAGG CCTTATTTCA AAAATAGTTA TTTTCAGTTC TTCTGGTTTG 
GTAAAACGTG AATTTGAACA AAAAATCACA AAACCCAAGG AACCACCAAG AGCCATTATT 
AAACAGAAAT GTTCAAGTTT TAGGAAGTTA AAAAGGAAAG AAGAAAAAAA AATACGAAGA 
AAACGTGCAC GGGAAGATGC ACTGAATTCT TCTAAACAGA AAATAGAAGA AAATCAGATG 
CGAAATGAAC TGTCTCAGTA CCGTAATCGC AGTGATTATG CAGGTTATAC TGGTGATCAA 
GTCCTGCAGC GTATCATTCA AAATGCTGAG CAAATAAAGA CTGCTGTTCG TGATGCCTCC 
AAACTGTTAA AGGAATTACT TTCCTGGTTT GTAATCAGTG AAGAGGATTA CACATCATAT 
TGCAAAACTA GCAGCATGTC ACATGCTGTG GTGGAGCAGC TCATCGGTTA CTTGATTCAG 
GAAGAAAACA GAGTGAAAAC AAGGAGTTTT ATCCGTGTCC TGAGTGAAAT GGAAGAAGTG 
GATCCCAAAT TACAGAGGTG GATGAAACAC CTTAATGATT TGGGGTTGAG AGCTACAGTG 
AACTTTATCC ATCAGTATGG ACAATTTTTA AAACAACTTG ACTTTTCTAT TTTATCTGCA 
CCCTGGAATG AGAAGTATGG TAGTAAATTT GACTACGTGT CAAGTAGTTC TGAAGGCAAA 
GAAATTCTTG ACTATTTTTT AAAACCACCT CTAGAAAAGG TCCGTTGTTT TATATGGCTG 
TTAGAAGACA ACAGAGAACA TTTTCCATCT CTTCATCAAG CTTTAGATGA ATTCTTTGAT 
AAAACGGACA ACCCTACCAT CATATTAAAG AAGCATGGGA ATGAAGATAT ATTGACTAAT 
GGTATCAAAG TTAAAAACAA AAGCAGAAAG AAGAACTATA AAGACTCACG GTCTGTTATA 
GTGTTATCCA GTGGAGTTAG TACAGCACCA CAAGAAGAAA ATACATTTAT AGAAGAAACT 
ACATTAGATT GTACGTATTA TGAACCATGT ATTATCCCAG AATATCTTCG GAAGCAACTA 
GAGGAGTTTG AATCCGTGTA TGACATTTCA AACAGTAACA GCTATCAAAG ACTGGATGAT 
AATCTAAATC AAACCTGTGA AAGTTTATAT GACTATTTCT CTCAGATATT GGAAGAGCAT 
GGACCGATGG AATTAAATAA TAAGTTGCTG GTTGGGGAAT ATGAACATTT CCCTGAAGAA 
GCTCGAAAGA TTGTAGAAGA TGAGGGTGGT TTGAAATCTT TTCTTCTGAA ATCCCATCGT 
TTCGTCATGG TGGATAATTT TATTAGCTTA AGACATGATG TTCTTATTAA AGAAAATAAA 
AACAGAGACG AGACTGAAAA TAATGAAGAA GGAAATTATA TTTTCTGTGA TGCGCAAGAA 
AATTCTTTCC AGAACAAGAT ACAGTTAAAT CCAGCTGCTA AGGAATTCAA ACCACTGTCT 
TATCCTGAAC AACCTCATCT ATCAACATCT ACTGACATAT CATTAGAAAG CTGTGAGACT 
CCACAATGTT TGCCCTACTC TTTGCCTACT TCATGGCAAT CAATGCTTAG TCAGGATATT 
GATCCTGTCG AAAATACGCC TTATTTTTCA AATGTTTTAT TGTATCAAGA TTTTCAGAAT 
CAAAGCAGTT TTTTGCCTCA GACTTCCTGG GGTTATCAGT GTGAAAGAAC ATCGCCAGTG 
CCTTCTCCAG TGCCTCTCCT GTCAAATGTT GCCAAGCAAC CTGGTTATAT TTATGCTGAT 
TGTGTCGCTC ATCAGAATAA TGATGAATTA GCAATTTCAG ACAGAAATTA TGTCAGCAAC 
AGCTTTATAC CAGCTGAAAT AGAAACATAC AGAGACCCTC AGATCCTGGT GGAGAACTCG 
GATAACGCAT TTTATGAGGA CAATTTTGCT GTTGCAAATA GTACAAGTGA AGCTGAATGT 
GATACTCAGG TGATTAAGAA GGAAAAAGAA AGCAGAGGTA TACCTGTAAT GAAAAACAAT 
CCGCACACGA GGATGGTAGC TGTTCAGGTG AATAGTGAAG TAACTGATAG GGAAACAAAT 
ACTTTGCCTT TTCATCCATT TGAGAAGCAA CAAGGAGATA TTCTACGTAT GGAAAAAGAG 
CATCAAGTAT TAAAAGAGCA ACTTAAAGAA GCACAGGAAA AATACGAACA ATTACAGAAC 
CGGAGCTCAG AGGAAATCAG TGTTTTAGAA GAGCTGATAA AGAAGATTGT TGAAGAGAAA 
GGGATATTTA AGAAAGAAGT GGAGTGGCTT CATCAATCTT TAGAAATAAA AGTAAAAAAA 
TGGCAACAGG AGAAAAAGGA AAATCAAGAG AACTTAAAAG CAATGAGGAA CACAGCCAAA 
AAGCATATGG ATACCAATGA CAGGTATTCA AAGACCATTG ATGAGAAGGA GAAGCAGTAT 
AACACATGTT TGAATACTTA TCTTGAGACC AGTAATAAAT TTGCTAATGA AAAAGTAAAA 
TTGGAAGAAC TTATTAAAAA GAGTCAAGAT GACCTCAAGG AGCATGAGAA AAGAGCTGTT 
ACAGCAGAGA TATCAGTACT CAAAAACTGG AAAGAAAGCG AACTGTGCAA ACTAAGTGGC 
ATAGTTGCCA GAGCAGAAAC AAATCTCAAG ATGTTGAAGT CACTAAGCAG CAACTCAGCT 
TCAGCGCTTC CTGACTTAAA GTCACAGATT AATTATTGGG AAGTATTTAT TTCAAATATA 
AAGAAACAAA TGGAGAAAGT AAAGGTTGGG TACGAAGAAA AAATTGAGAA GGTGAAAAAT 
GGTGTTCGAA ACTGCCTCAC CAAAATAGAA GCTGCGGATC TTTCATCTCT TCCCAGTGTC 
TTAAGAAAGC AAAGCAGACC TGCTGTCAGT GATCCAGCCC TTGTCCTGTG TTCCTCTGCA 
TCTGCACAGC CTAATTTACT TCCTGCAGTT TCGAGTTCTG AAGATCAAGG TCCAACAACT 
GCTTCATTTA ATAGCCAGGC TGGAGTTAAG CCTGCAAATG CAAATTCTAA GGTTTGCTCT 
GCAAGTGATG GATCAGTGAA AACAAACTCC AAAGCTCGGA CAGGATCGTA TGCTGATAAA 
GTTTCGCTAA ATTCTATGCC AAAGGTTTCA GGAAGCAATA CTCAGAATAT ACAGCCAAAC 
CAAACCCACC AAGATGATTC AGCTACACAG ATGAAGCCCA CTTCAAACAA CACATTTGAA 
AATATTATTG CTCATCTACA AACTATATTT CCGGGCTACA GCAGTTCAGA GTTCACGAGG 
TTCATAAAAG ATGTACAAAG AAGAAGTGGG AATACATTAA GTTTGAGCTC CACTGAAATT 
CTAAGCAGAG TGACAGATCT CATTCTGGAC CAGCAAAATG AGGCATCAGC TTCTGTAGGG 
AGACCTATGA TGTCTGCATC ATCCGCTTCA GCGGGACAGA CGGGAACTCA AAGTCAGGAA 
GCAGCTGCAG AAGGAAATAT TTCCAGCGCA CCCAAGGCAA GGCCTGCACA CAAAAGCACC 
TCCAGTAAAA ACACGCTGCC ATCTCAGCCA AATCTCCAGC CATGGGGGAA TGCTGGAGCA 
ACACCGAAAT CTAAATGGAA AAAACTAGAC TATATACCTT CCAGCGATGA TCCGTGCACC 
ATCTGCCATG ATGAGTTAAG CAGAGACTCA TGTGAACTGG AGTGTGGGCA TCATTTTCAC 
AGAGAATGCA TTAGAAAATG GCTGAAGGAA CATTCGAGTA CCTGCCCTAT CTGTCGCATC 
CATGTTCTGT TACCTGAAGA CTTTCCTGAG CTCCCTGCAC GCAACAAATA TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156894.1
CDS	65..6001
Translation	MVGAGARTMGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301408.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301408.2

ORF Insert Sequence:

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGTT TACAGATTTT AGACTGGAAT 
TGGAAGAAAC TCTGTGATTT AGAGCTTGTG GAAGACCTTG TTGATTTGAC TAAAAAAGTG 
GTTAATGTTC CACATTTTAT TGGGGGCATA CTGAGAATTG GCAGCAGGTT AGAAAACGGT 
TTCTTCGATG TTAGGGATGC ACTTGATTGG ATCAGATGTA CAGGTGATAT CAATATTCTG 
CAGAAGCTGG AGAAACTTGG TGATTTTGGT TGGCTCTATC TTGAAACTTT CTTTGGTGAA 
TGCAAACATT ATATTACTAA AGTTGCTTTG GAAGATTGTA ATCTAGTTGA AGAATTTAAA 
GCTGTCACCT GTGAAAACTG CAGGAAGAAG AGTGAATCTA TGAAACAGAA AGGAAATGAG 
GAGTTCTCCC AAGGAAAATA TGATCATGCT ATAATATCAT ATACTAAAGC CATAGAGTTT 
TGTCCTGCAA ACCATCTTCT GTATGGAAAC CGAGCTCTTT GTTTAATTCT CACGAAGCAG 
TACAAGAGAG CCCTTGCTGA TGGAAAAAGG GCCACTATTT TGAAGCCTAA TTGGCCAAAG 
GGTCATTACC ACTTCTGTAA GGCGCTGTCA TTACTGGGGG AGCATGAACT GGCTTTAGAA 
GCTAATGAGA GAGCTCAGGA ACTGTGCAAG AATATTCTTG ATGGATTTAA GGATCTTATT 
CAACAAAATG ACAAGTTAAA GAAGACATTA GAGGAAATCA AGGGTACTAA AGAATATAAG 
CAGAAACCAA AGAATTTGCT TCTTGAGAAA AAGGACTGTG GTTCTTCAAA ATCGCATTTG 
ATCGTTTCTC AAGAACAAAA AGAAATAGAG AAGGACAGAA AACAAATGCA GTTTGATCAT 
AAAGATGACC ATTATCATCA AAAACACGAT ACCACCATAA GATATCCTAC AGATGGAATT 
CCAAGACTAT CAGTGACCAA CATGCCCAGG ACAGAAAACA AAGTGTGGTC TCTGGACTTT 
CAACCAAGCC AAAGTCACCA ACACAAAGGA AGGTCGAAGA ACAAGCCTGG TGAACCTGAA 
AAGAAACAGG ATCACCTTGA TTTGAAGATA GACTCTAAAA GCATCCAAGA TAAACTCGTC 
TGTACTGTGC ACCAGGTGGA TTCAACTATG TTGCTTGAAG TGGTTAAATC CCTCGTTCAT 
GATGGTTACA CAGCCTTGAT GGACCAGAGG TGTCACAGTG CTGAACAAAT GTTCTCACAA 
CTGTTGAACA TTCTCAATCC TTCGGAATTA AAGCAATTGA ATCTTCGTGT TATTAATTAT 
GTTGTAATCA TCTATGGACA TGCCACTTCT CTGCTTGGAA TAGGACAGCC CGAGGCGTTG 
ACAAAGGCTG AAGACCAGTT TAAAAGAATA ATTATACAGT ACCAGCAAGA AAGATTTGAT 
TGTCTGGCAT ACTATGGAAT TGGAAAAGTA TACCTCAGAC AAAACAGATT TTTAGATGCG 
CTCAATCAGT TTAAGAAGTC ACAAACAATG ATTAATCATA AGATTGTACC TGGAGTATTA 
ACTTGGCCCA CGACATCTCA GGTCATTGAA GAAACACGAA CAGAGAATTT ACAGTTGTTC 
TTGAAGAACT GCATTGAGGA ATGCAAGTTC CCTCCAGAAC CGGATGCAAT TTGTCGCTAT 
CAGCAGTGCC ATGGCCACTC CAAAATCCAA ATATTTTTTA CAGATCCAGA CTTTAAGGGT 
TTTATACGTA TTACTTGCTG TCAGCAATGT AGAGTCGAGT TTCATACCAG CTGTTGGAAA 
AAGTTGAAAA CTGCAAACTA CAGTGATAAG AATGACAAGG ATTTTCTTCA AGAATTATGT 
TTCACTCCCG ACTGTAGAGG CCTTATTTCA AAAATAGTTA TTTTCAGTTC TTCTGGTTTG 
GTAAAACGTG AATTTGAACA AAAAATCACA AAACCCAAGG AACCACCAAG AGCCATTATT 
AAACAGAAAT GTTCAAGTTT TAGGAAGTTA AAAAGGAAAG AAGAAAAAAA AATACGAAGA 
AAACGTGCAC GGGAAGATGC ACTGAATTCT TCTAAACAGA AAATAGAAGA AAATCAGATG 
CGAAATGAAC TGTCTCAGTA CCGTAATCGC AGTGATTATG CAGGTTATAC TGGTGATCAA 
GTCCTGCAGC GTATCATTCA AAATGCTGAG CAAATAAAGA CTGCTGTTCG TGATGCCTCC 
AAACTGTTAA AGGAATTACT TTCCTGGTTT GTAATCAGTG AAGAGGATTA CACATCATAT 
TGCAAAACTA GCAGCATGTC ACATGCTGTG GTGGAGCAGC TCATCGGTTA CTTGATTCAG 
GAAGAAAACA GAGTGAAAAC AAGGAGTTTT ATCCGTGTCC TGAGTGAAAT GGAAGAAGTG 
GATCCCAAAT TACAGAGGTG GATGAAACAC CTTAATGATT TGGGGTTGAG AGCTACAGTG 
AACTTTATCC ATCAGTATGG ACAATTTTTA AAACAACTTG ACTTTTCTAT TTTATCTGCA 
CCCTGGAATG AGAAGTATGG TAGTAAATTT GACTACGTGT CAAGTAGTTC TGAAGGCAAA 
GAAATTCTTG ACTATTTTTT AAAACCACCT CTAGAAAAGG TCCGTTGTTT TATATGGCTG 
TTAGAAGACA ACAGAGAACA TTTTCCATCT CTTCATCAAG CTTTAGATGA ATTCTTTGAT 
AAAACGGACA ACCCTACCAT CATATTAAAG AAGCATGGGA ATGAAGATAT ATTGACTAAT 
GGTATCAAAG TTAAAAACAA AAGCAGAAAG AAGAACTATA AAGACTCACG GTCTGTTATA 
GTGTTATCCA GTGGAGTTAG TACAGCACCA CAAGAAGAAA ATACATTTAT AGAAGAAACT 
ACATTAGATT GTACGTATTA TGAACCATGT ATTATCCCAG AATATCTTCG GAAGCAACTA 
GAGGAGTTTG AATCCGTGTA TGACATTTCA AACAGTAACA GCTATCAAAG ACTGGATGAT 
AATCTAAATC AAACCTGTGA AAGTTTATAT GACTATTTCT CTCAGATATT GGAAGAGCAT 
GGACCGATGG AATTAAATAA TAAGTTGCTG GTTGGGGAAT ATGAACATTT CCCTGAAGAA 
GCTCGAAAGA TTGTAGAAGA TGAGGGTGGT TTGAAATCTT TTCTTCTGAA ATCCCATCGT 
TTCGTCATGG TGGATAATTT TATTAGCTTA AGACATGATG TTCTTATTAA AGAAAATAAA 
AACAGAGACG AGACTGAAAA TAATGAAGAA GGAAATTATA TTTTCTGTGA TGCGCAAGAA 
AATTCTTTCC AGAACAAGAT ACAGTTAAAT CCAGCTGCTA AGGAATTCAA ACCACTGTCT 
TATCCTGAAC AACCTCATCT ATCAACATCT ACTGACATAT CATTAGAAAG CTGTGAGACT 
CCACAATGTT TGCCCTACTC TTTGCCTACT TCATGGCAAT CAATGCTTAG TCAGGATATT 
GATCCTGTCG AAAATACGCC TTATTTTTCA AATGTTTTAT TGTATCAAGA TTTTCAGAAT 
CAAAGCAGTT TTTTGCCTCA GACTTCCTGG GGTTATCAGT GTGAAAGAAC ATCGCCAGTG 
CCTTCTCCAG TGCCTCTCCT GTCAAATGTT GCCAAGCAAC CTGGTTATAT TTATGCTGAT 
TGTGTCGCTC ATCAGAATAA TGATGAATTA GCAATTTCAG ACAGAAATTA TGTCAGCAAC 
AGCTTTATAC CAGCTGAAAT AGAAACATAC AGAGACCCTC AGATCCTGGT GGAGAACTCG 
GATAACGCAT TTTATGAGGA CAATTTTGCT GTTGCAAATA GTACAAGTGA AGCTGAATGT 
GATACTCAGG TGATTAAGAA GGAAAAAGAA AGCAGAGGTA TACCTGTAAT GAAAAACAAT 
CCGCACACGA GGATGGTAGC TGTTCAGGTG AATAGTGAAG TAACTGATAG GGAAACAAAT 
ACTTTGCCTT TTCATCCATT TGAGAAGCAA CAAGGAGATA TTCTACGTAT GGAAAAAGAG 
CATCAAGTAT TAAAAGAGCA ACTTAAAGAA GCACAGGAAA AATACGAACA ATTACAGAAC 
CGGAGCTCAG AGGAAATCAG TGTTTTAGAA GAGCTGATAA AGAAGATTGT TGAAGAGAAA 
GGGATATTTA AGAAAGAAGT GGAGTGGCTT CATCAATCTT TAGAAATAAA AGTAAAAAAA 
TGGCAACAGG AGAAAAAGGA AAATCAAGAG AACTTAAAAG CAATGAGGAA CACAGCCAAA 
AAGCATATGG ATACCAATGA CAGGTATTCA AAGACCATTG ATGAGAAGGA GAAGCAGTAT 
AACACATGTT TGAATACTTA TCTTGAGACC AGTAATAAAT TTGCTAATGA AAAAGTAAAA 
TTGGAAGAAC TTATTAAAAA GAGTCAAGAT GACCTCAAGG AGCATGAGAA AAGAGCTGTT 
ACAGCAGAGA TATCAGTACT CAAAAACTGG AAAGAAAGCG AACTGTGCAA ACTAAGTGGC 
ATAGTTGCCA GAGCAGAAAC AAATCTCAAG ATGTTGAAGT CACTAAGCAG CAACTCAGCT 
TCAGCGCTTC CTGACTTAAA GTCACAGATT AATTATTGGG AAGTATTTAT TTCAAATATA 
AAGAAACAAA TGGAGAAAGT AAAGGTTGGG TACGAAGAAA AAATTGAGAA GGTGAAAAAT 
GGTGTTCGAA ACTGCCTCAC CAAAATAGAA GCTGCGGATC TTTCATCTCT TCCCAGTGTC 
TTAAGAAAGC AAAGCAGACC TGCTGTCAGT GATCCAGCCC TTGTCCTGTG TTCCTCTGCA 
TCTGCACAGC CTAATTTACT TCCTGCAGTT TCGAGTTCTG AAGATCAAGG TCCAACAACT 
GCTTCATTTA ATAGCCAGGC TGGAGTTAAG CCTGCAAATG CAAATTCTAA GGTTTGCTCT 
GCAAGTGATG GATCAGTGAA AACAAACTCC AAAGCTCGGA CAGGATCGTA TGCTGATAAA 
GTTTCGCTAA ATTCTATGCC AAAGGTTTCA GGAAGCAATA CTCAGAATAT ACAGCCAAAC 
CAAACCCACC AAGATGATTC AGCTACACAG ATGAAGCCCA CTTCAAACAA CACATTTGAA 
AATATTATTG CTCATCTACA AACTATATTT CCGGGCTACA GCAGTTCAGA GTTCACGAGG 
TTCATAAAAG ATGTACAAAG AAGAAGTGGG AATACATTAA GTTTGAGCTC CACTGAAATT 
CTAAGCAGAG TGACAGATCT CATTCTGGAC CAGCAAAATG AGGCATCAGC TTCTGTAGGG 
AGACCTATGA TGTCTGCATC ATCCGCTTCA GCGGGACAGA CGGGAACTCA AAGTCAGGAA 
GCAGCTGCAG AAGGAAATAT TTCCAGCGCA CCCAAGGCAA GGCCTGCACA CAAAAGCACC 
TCCAGTAAAA ACACGCTGCC ATCTCAGCCA AATCTCCAGC CATGGGGGAA TGCTGGAGCA 
ACACCGAAAT CTAAATGGAA AAAACTAGAC TATATACCTT CCAGCGATGA TCCGTGCACC 
ATCTGCCATG ATGAGTTAAG CAGAGACTCA TGTGAACTGG AGTGTGGGCA TCATTTTCAC 
AGAGAATGCA TTAGAAAATG GCTGAAGGAA CATTCGAGTA CCTGCCCTAT CTGTCGCATC 
CATGTTCTGT TACCTGAAGA CTTTCCTGAG CTCCCTGCAC GCAACAAATA TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301408.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156894.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

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CloneID	OGa30356
Clone ID Related Accession (Same CDS sequence)	XM_015301408.2 , XM_015301408.1
Accession Version	XM_015301408.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGTT TACAGATTTT AGACTGGAAT 
TGGAAGAAAC TCTGTGATTT AGAGCTTGTG GAAGACCTTG TTGATTTGAC TAAAAAAGTG 
GTTAATGTTC CACATTTTAT TGGGGGCATA CTGAGAATTG GCAGCAGGTT AGAAAACGGT 
TTCTTCGATG TTAGGGATGC ACTTGATTGG ATCAGATGTA CAGGTGATAT CAATATTCTG 
CAGAAGCTGG AGAAACTTGG TGATTTTGGT TGGCTCTATC TTGAAACTTT CTTTGGTGAA 
TGCAAACATT ATATTACTAA AGTTGCTTTG GAAGATTGTA ATCTAGTTGA AGAATTTAAA 
GCTGTCACCT GTGAAAACTG CAGGAAGAAG AGTGAATCTA TGAAACAGAA AGGAAATGAG 
GAGTTCTCCC AAGGAAAATA TGATCATGCT ATAATATCAT ATACTAAAGC CATAGAGTTT 
TGTCCTGCAA ACCATCTTCT GTATGGAAAC CGAGCTCTTT GTTTAATTCT CACGAAGCAG 
TACAAGAGAG CCCTTGCTGA TGGAAAAAGG GCCACTATTT TGAAGCCTAA TTGGCCAAAG 
GGTCATTACC ACTTCTGTAA GGCGCTGTCA TTACTGGGGG AGCATGAACT GGCTTTAGAA 
GCTAATGAGA GAGCTCAGGA ACTGTGCAAG AATATTCTTG ATGGATTTAA GGATCTTATT 
CAACAAAATG ACAAGTTAAA GAAGACATTA GAGGAAATCA AGGGTACTAA AGAATATAAG 
CAGAAACCAA AGAATTTGCT TCTTGAGAAA AAGGACTGTG GTTCTTCAAA ATCGCATTTG 
ATCGTTTCTC AAGAACAAAA AGAAATAGAG AAGGACAGAA AACAAATGCA GTTTGATCAT 
AAAGATGACC ATTATCATCA AAAACACGAT ACCACCATAA GATATCCTAC AGATGGAATT 
CCAAGACTAT CAGTGACCAA CATGCCCAGG ACAGAAAACA AAGTGTGGTC TCTGGACTTT 
CAACCAAGCC AAAGTCACCA ACACAAAGGA AGGTCGAAGA ACAAGCCTGG TGAACCTGAA 
AAGAAACAGG ATCACCTTGA TTTGAAGATA GACTCTAAAA GCATCCAAGA TAAACTCGTC 
TGTACTGTGC ACCAGGTGGA TTCAACTATG TTGCTTGAAG TGGTTAAATC CCTCGTTCAT 
GATGGTTACA CAGCCTTGAT GGACCAGAGG TGTCACAGTG CTGAACAAAT GTTCTCACAA 
CTGTTGAACA TTCTCAATCC TTCGGAATTA AAGCAATTGA ATCTTCGTGT TATTAATTAT 
GTTGTAATCA TCTATGGACA TGCCACTTCT CTGCTTGGAA TAGGACAGCC CGAGGCGTTG 
ACAAAGGCTG AAGACCAGTT TAAAAGAATA ATTATACAGT ACCAGCAAGA AAGATTTGAT 
TGTCTGGCAT ACTATGGAAT TGGAAAAGTA TACCTCAGAC AAAACAGATT TTTAGATGCG 
CTCAATCAGT TTAAGAAGTC ACAAACAATG ATTAATCATA AGATTGTACC TGGAGTATTA 
ACTTGGCCCA CGACATCTCA GGTCATTGAA GAAACACGAA CAGAGAATTT ACAGTTGTTC 
TTGAAGAACT GCATTGAGGA ATGCAAGTTC CCTCCAGAAC CGGATGCAAT TTGTCGCTAT 
CAGCAGTGCC ATGGCCACTC CAAAATCCAA ATATTTTTTA CAGATCCAGA CTTTAAGGGT 
TTTATACGTA TTACTTGCTG TCAGCAATGT AGAGTCGAGT TTCATACCAG CTGTTGGAAA 
AAGTTGAAAA CTGCAAACTA CAGTGATAAG AATGACAAGG ATTTTCTTCA AGAATTATGT 
TTCACTCCCG ACTGTAGAGG CCTTATTTCA AAAATAGTTA TTTTCAGTTC TTCTGGTTTG 
GTAAAACGTG AATTTGAACA AAAAATCACA AAACCCAAGG AACCACCAAG AGCCATTATT 
AAACAGAAAT GTTCAAGTTT TAGGAAGTTA AAAAGGAAAG AAGAAAAAAA AATACGAAGA 
AAACGTGCAC GGGAAGATGC ACTGAATTCT TCTAAACAGA AAATAGAAGA AAATCAGATG 
CGAAATGAAC TGTCTCAGTA CCGTAATCGC AGTGATTATG CAGGTTATAC TGGTGATCAA 
GTCCTGCAGC GTATCATTCA AAATGCTGAG CAAATAAAGA CTGCTGTTCG TGATGCCTCC 
AAACTGTTAA AGGAATTACT TTCCTGGTTT GTAATCAGTG AAGAGGATTA CACATCATAT 
TGCAAAACTA GCAGCATGTC ACATGCTGTG GTGGAGCAGC TCATCGGTTA CTTGATTCAG 
GAAGAAAACA GAGTGAAAAC AAGGAGTTTT ATCCGTGTCC TGAGTGAAAT GGAAGAAGTG 
GATCCCAAAT TACAGAGGTG GATGAAACAC CTTAATGATT TGGGGTTGAG AGCTACAGTG 
AACTTTATCC ATCAGTATGG ACAATTTTTA AAACAACTTG ACTTTTCTAT TTTATCTGCA 
CCCTGGAATG AGAAGTATGG TAGTAAATTT GACTACGTGT CAAGTAGTTC TGAAGGCAAA 
GAAATTCTTG ACTATTTTTT AAAACCACCT CTAGAAAAGG TCCGTTGTTT TATATGGCTG 
TTAGAAGACA ACAGAGAACA TTTTCCATCT CTTCATCAAG CTTTAGATGA ATTCTTTGAT 
AAAACGGACA ACCCTACCAT CATATTAAAG AAGCATGGGA ATGAAGATAT ATTGACTAAT 
GGTATCAAAG TTAAAAACAA AAGCAGAAAG AAGAACTATA AAGACTCACG GTCTGTTATA 
GTGTTATCCA GTGGAGTTAG TACAGCACCA CAAGAAGAAA ATACATTTAT AGAAGAAACT 
ACATTAGATT GTACGTATTA TGAACCATGT ATTATCCCAG AATATCTTCG GAAGCAACTA 
GAGGAGTTTG AATCCGTGTA TGACATTTCA AACAGTAACA GCTATCAAAG ACTGGATGAT 
AATCTAAATC AAACCTGTGA AAGTTTATAT GACTATTTCT CTCAGATATT GGAAGAGCAT 
GGACCGATGG AATTAAATAA TAAGTTGCTG GTTGGGGAAT ATGAACATTT CCCTGAAGAA 
GCTCGAAAGA TTGTAGAAGA TGAGGGTGGT TTGAAATCTT TTCTTCTGAA ATCCCATCGT 
TTCGTCATGG TGGATAATTT TATTAGCTTA AGACATGATG TTCTTATTAA AGAAAATAAA 
AACAGAGACG AGACTGAAAA TAATGAAGAA GGAAATTATA TTTTCTGTGA TGCGCAAGAA 
AATTCTTTCC AGAACAAGAT ACAGTTAAAT CCAGCTGCTA AGGAATTCAA ACCACTGTCT 
TATCCTGAAC AACCTCATCT ATCAACATCT ACTGACATAT CATTAGAAAG CTGTGAGACT 
CCACAATGTT TGCCCTACTC TTTGCCTACT TCATGGCAAT CAATGCTTAG TCAGGATATT 
GATCCTGTCG AAAATACGCC TTATTTTTCA AATGTTTTAT TGTATCAAGA TTTTCAGAAT 
CAAAGCAGTT TTTTGCCTCA GACTTCCTGG GGTTATCAGT GTGAAAGAAC ATCGCCAGTG 
CCTTCTCCAG TGCCTCTCCT GTCAAATGTT GCCAAGCAAC CTGGTTATAT TTATGCTGAT 
TGTGTCGCTC ATCAGAATAA TGATGAATTA GCAATTTCAG ACAGAAATTA TGTCAGCAAC 
AGCTTTATAC CAGCTGAAAT AGAAACATAC AGAGACCCTC AGATCCTGGT GGAGAACTCG 
GATAACGCAT TTTATGAGGA CAATTTTGCT GTTGCAAATA GTACAAGTGA AGCTGAATGT 
GATACTCAGG TGATTAAGAA GGAAAAAGAA AGCAGAGGTA TACCTGTAAT GAAAAACAAT 
CCGCACACGA GGATGGTAGC TGTTCAGGTG AATAGTGAAG TAACTGATAG GGAAACAAAT 
ACTTTGCCTT TTCATCCATT TGAGAAGCAA CAAGGAGATA TTCTACGTAT GGAAAAAGAG 
CATCAAGTAT TAAAAGAGCA ACTTAAAGAA GCACAGGAAA AATACGAACA ATTACAGAAC 
CGGAGCTCAG AGGAAATCAG TGTTTTAGAA GAGCTGATAA AGAAGATTGT TGAAGAGAAA 
GGGATATTTA AGAAAGAAGT GGAGTGGCTT CATCAATCTT TAGAAATAAA AGTAAAAAAA 
TGGCAACAGG AGAAAAAGGA AAATCAAGAG AACTTAAAAG CAATGAGGAA CACAGCCAAA 
AAGCATATGG ATACCAATGA CAGGTATTCA AAGACCATTG ATGAGAAGGA GAAGCAGTAT 
AACACATGTT TGAATACTTA TCTTGAGACC AGTAATAAAT TTGCTAATGA AAAAGTAAAA 
TTGGAAGAAC TTATTAAAAA GAGTCAAGAT GACCTCAAGG AGCATGAGAA AAGAGCTGTT 
ACAGCAGAGA TATCAGTACT CAAAAACTGG AAAGAAAGCG AACTGTGCAA ACTAAGTGGC 
ATAGTTGCCA GAGCAGAAAC AAATCTCAAG ATGTTGAAGT CACTAAGCAG CAACTCAGCT 
TCAGCGCTTC CTGACTTAAA GTCACAGATT AATTATTGGG AAGTATTTAT TTCAAATATA 
AAGAAACAAA TGGAGAAAGT AAAGGTTGGG TACGAAGAAA AAATTGAGAA GGTGAAAAAT 
GGTGTTCGAA ACTGCCTCAC CAAAATAGAA GCTGCGGATC TTTCATCTCT TCCCAGTGTC 
TTAAGAAAGC AAAGCAGACC TGCTGTCAGT GATCCAGCCC TTGTCCTGTG TTCCTCTGCA 
TCTGCACAGC CTAATTTACT TCCTGCAGTT TCGAGTTCTG AAGATCAAGG TCCAACAACT 
GCTTCATTTA ATAGCCAGGC TGGAGTTAAG CCTGCAAATG CAAATTCTAA GGTTTGCTCT 
GCAAGTGATG GATCAGTGAA AACAAACTCC AAAGCTCGGA CAGGATCGTA TGCTGATAAA 
GTTTCGCTAA ATTCTATGCC AAAGGTTTCA GGAAGCAATA CTCAGAATAT ACAGCCAAAC 
CAAACCCACC AAGATGATTC AGCTACACAG ATGAAGCCCA CTTCAAACAA CACATTTGAA 
AATATTATTG CTCATCTACA AACTATATTT CCGGGCTACA GCAGTTCAGA GTTCACGAGG 
TTCATAAAAG ATGTACAAAG AAGAAGTGGG AATACATTAA GTTTGAGCTC CACTGAAATT 
CTAAGCAGAG TGACAGATCT CATTCTGGAC CAGCAAAATG AGGCATCAGC TTCTGTAGGG 
AGACCTATGA TGTCTGCATC ATCCGCTTCA GCGGGACAGA CGGGAACTCA AAGTCAGGAA 
GCAGCTGCAG AAGGAAATAT TTCCAGCGCA CCCAAGGCAA GGCCTGCACA CAAAAGCACC 
TCCAGTAAAA ACACGCTGCC ATCTCAGCCA AATCTCCAGC CATGGGGGAA TGCTGGAGCA 
ACACCGAAAT CTAAATGGAA AAAACTAGAC TATATACCTT CCAGCGATGA TCCGTGCACC 
ATCTGCCATG ATGAGTTAAG CAGAGACTCA TGTGAACTGG AGTGTGGGCA TCATTTTCAC 
AGAGAATGCA TTAGAAAATG GCTGAAGGAA CATTCGAGTA CCTGCCCTAT CTGTCGCATC 
CATGTTCTGT TACCTGAAGA CTTTCCTGAG CTCCCTGCAC GCAACAAATA TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156894.1
CDS	99..6035
Translation	MVGAGARTMGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301408.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301408.1

ORF Insert Sequence:

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGTT TACAGATTTT AGACTGGAAT 
TGGAAGAAAC TCTGTGATTT AGAGCTTGTG GAAGACCTTG TTGATTTGAC TAAAAAAGTG 
GTTAATGTTC CACATTTTAT TGGGGGCATA CTGAGAATTG GCAGCAGGTT AGAAAACGGT 
TTCTTCGATG TTAGGGATGC ACTTGATTGG ATCAGATGTA CAGGTGATAT CAATATTCTG 
CAGAAGCTGG AGAAACTTGG TGATTTTGGT TGGCTCTATC TTGAAACTTT CTTTGGTGAA 
TGCAAACATT ATATTACTAA AGTTGCTTTG GAAGATTGTA ATCTAGTTGA AGAATTTAAA 
GCTGTCACCT GTGAAAACTG CAGGAAGAAG AGTGAATCTA TGAAACAGAA AGGAAATGAG 
GAGTTCTCCC AAGGAAAATA TGATCATGCT ATAATATCAT ATACTAAAGC CATAGAGTTT 
TGTCCTGCAA ACCATCTTCT GTATGGAAAC CGAGCTCTTT GTTTAATTCT CACGAAGCAG 
TACAAGAGAG CCCTTGCTGA TGGAAAAAGG GCCACTATTT TGAAGCCTAA TTGGCCAAAG 
GGTCATTACC ACTTCTGTAA GGCGCTGTCA TTACTGGGGG AGCATGAACT GGCTTTAGAA 
GCTAATGAGA GAGCTCAGGA ACTGTGCAAG AATATTCTTG ATGGATTTAA GGATCTTATT 
CAACAAAATG ACAAGTTAAA GAAGACATTA GAGGAAATCA AGGGTACTAA AGAATATAAG 
CAGAAACCAA AGAATTTGCT TCTTGAGAAA AAGGACTGTG GTTCTTCAAA ATCGCATTTG 
ATCGTTTCTC AAGAACAAAA AGAAATAGAG AAGGACAGAA AACAAATGCA GTTTGATCAT 
AAAGATGACC ATTATCATCA AAAACACGAT ACCACCATAA GATATCCTAC AGATGGAATT 
CCAAGACTAT CAGTGACCAA CATGCCCAGG ACAGAAAACA AAGTGTGGTC TCTGGACTTT 
CAACCAAGCC AAAGTCACCA ACACAAAGGA AGGTCGAAGA ACAAGCCTGG TGAACCTGAA 
AAGAAACAGG ATCACCTTGA TTTGAAGATA GACTCTAAAA GCATCCAAGA TAAACTCGTC 
TGTACTGTGC ACCAGGTGGA TTCAACTATG TTGCTTGAAG TGGTTAAATC CCTCGTTCAT 
GATGGTTACA CAGCCTTGAT GGACCAGAGG TGTCACAGTG CTGAACAAAT GTTCTCACAA 
CTGTTGAACA TTCTCAATCC TTCGGAATTA AAGCAATTGA ATCTTCGTGT TATTAATTAT 
GTTGTAATCA TCTATGGACA TGCCACTTCT CTGCTTGGAA TAGGACAGCC CGAGGCGTTG 
ACAAAGGCTG AAGACCAGTT TAAAAGAATA ATTATACAGT ACCAGCAAGA AAGATTTGAT 
TGTCTGGCAT ACTATGGAAT TGGAAAAGTA TACCTCAGAC AAAACAGATT TTTAGATGCG 
CTCAATCAGT TTAAGAAGTC ACAAACAATG ATTAATCATA AGATTGTACC TGGAGTATTA 
ACTTGGCCCA CGACATCTCA GGTCATTGAA GAAACACGAA CAGAGAATTT ACAGTTGTTC 
TTGAAGAACT GCATTGAGGA ATGCAAGTTC CCTCCAGAAC CGGATGCAAT TTGTCGCTAT 
CAGCAGTGCC ATGGCCACTC CAAAATCCAA ATATTTTTTA CAGATCCAGA CTTTAAGGGT 
TTTATACGTA TTACTTGCTG TCAGCAATGT AGAGTCGAGT TTCATACCAG CTGTTGGAAA 
AAGTTGAAAA CTGCAAACTA CAGTGATAAG AATGACAAGG ATTTTCTTCA AGAATTATGT 
TTCACTCCCG ACTGTAGAGG CCTTATTTCA AAAATAGTTA TTTTCAGTTC TTCTGGTTTG 
GTAAAACGTG AATTTGAACA AAAAATCACA AAACCCAAGG AACCACCAAG AGCCATTATT 
AAACAGAAAT GTTCAAGTTT TAGGAAGTTA AAAAGGAAAG AAGAAAAAAA AATACGAAGA 
AAACGTGCAC GGGAAGATGC ACTGAATTCT TCTAAACAGA AAATAGAAGA AAATCAGATG 
CGAAATGAAC TGTCTCAGTA CCGTAATCGC AGTGATTATG CAGGTTATAC TGGTGATCAA 
GTCCTGCAGC GTATCATTCA AAATGCTGAG CAAATAAAGA CTGCTGTTCG TGATGCCTCC 
AAACTGTTAA AGGAATTACT TTCCTGGTTT GTAATCAGTG AAGAGGATTA CACATCATAT 
TGCAAAACTA GCAGCATGTC ACATGCTGTG GTGGAGCAGC TCATCGGTTA CTTGATTCAG 
GAAGAAAACA GAGTGAAAAC AAGGAGTTTT ATCCGTGTCC TGAGTGAAAT GGAAGAAGTG 
GATCCCAAAT TACAGAGGTG GATGAAACAC CTTAATGATT TGGGGTTGAG AGCTACAGTG 
AACTTTATCC ATCAGTATGG ACAATTTTTA AAACAACTTG ACTTTTCTAT TTTATCTGCA 
CCCTGGAATG AGAAGTATGG TAGTAAATTT GACTACGTGT CAAGTAGTTC TGAAGGCAAA 
GAAATTCTTG ACTATTTTTT AAAACCACCT CTAGAAAAGG TCCGTTGTTT TATATGGCTG 
TTAGAAGACA ACAGAGAACA TTTTCCATCT CTTCATCAAG CTTTAGATGA ATTCTTTGAT 
AAAACGGACA ACCCTACCAT CATATTAAAG AAGCATGGGA ATGAAGATAT ATTGACTAAT 
GGTATCAAAG TTAAAAACAA AAGCAGAAAG AAGAACTATA AAGACTCACG GTCTGTTATA 
GTGTTATCCA GTGGAGTTAG TACAGCACCA CAAGAAGAAA ATACATTTAT AGAAGAAACT 
ACATTAGATT GTACGTATTA TGAACCATGT ATTATCCCAG AATATCTTCG GAAGCAACTA 
GAGGAGTTTG AATCCGTGTA TGACATTTCA AACAGTAACA GCTATCAAAG ACTGGATGAT 
AATCTAAATC AAACCTGTGA AAGTTTATAT GACTATTTCT CTCAGATATT GGAAGAGCAT 
GGACCGATGG AATTAAATAA TAAGTTGCTG GTTGGGGAAT ATGAACATTT CCCTGAAGAA 
GCTCGAAAGA TTGTAGAAGA TGAGGGTGGT TTGAAATCTT TTCTTCTGAA ATCCCATCGT 
TTCGTCATGG TGGATAATTT TATTAGCTTA AGACATGATG TTCTTATTAA AGAAAATAAA 
AACAGAGACG AGACTGAAAA TAATGAAGAA GGAAATTATA TTTTCTGTGA TGCGCAAGAA 
AATTCTTTCC AGAACAAGAT ACAGTTAAAT CCAGCTGCTA AGGAATTCAA ACCACTGTCT 
TATCCTGAAC AACCTCATCT ATCAACATCT ACTGACATAT CATTAGAAAG CTGTGAGACT 
CCACAATGTT TGCCCTACTC TTTGCCTACT TCATGGCAAT CAATGCTTAG TCAGGATATT 
GATCCTGTCG AAAATACGCC TTATTTTTCA AATGTTTTAT TGTATCAAGA TTTTCAGAAT 
CAAAGCAGTT TTTTGCCTCA GACTTCCTGG GGTTATCAGT GTGAAAGAAC ATCGCCAGTG 
CCTTCTCCAG TGCCTCTCCT GTCAAATGTT GCCAAGCAAC CTGGTTATAT TTATGCTGAT 
TGTGTCGCTC ATCAGAATAA TGATGAATTA GCAATTTCAG ACAGAAATTA TGTCAGCAAC 
AGCTTTATAC CAGCTGAAAT AGAAACATAC AGAGACCCTC AGATCCTGGT GGAGAACTCG 
GATAACGCAT TTTATGAGGA CAATTTTGCT GTTGCAAATA GTACAAGTGA AGCTGAATGT 
GATACTCAGG TGATTAAGAA GGAAAAAGAA AGCAGAGGTA TACCTGTAAT GAAAAACAAT 
CCGCACACGA GGATGGTAGC TGTTCAGGTG AATAGTGAAG TAACTGATAG GGAAACAAAT 
ACTTTGCCTT TTCATCCATT TGAGAAGCAA CAAGGAGATA TTCTACGTAT GGAAAAAGAG 
CATCAAGTAT TAAAAGAGCA ACTTAAAGAA GCACAGGAAA AATACGAACA ATTACAGAAC 
CGGAGCTCAG AGGAAATCAG TGTTTTAGAA GAGCTGATAA AGAAGATTGT TGAAGAGAAA 
GGGATATTTA AGAAAGAAGT GGAGTGGCTT CATCAATCTT TAGAAATAAA AGTAAAAAAA 
TGGCAACAGG AGAAAAAGGA AAATCAAGAG AACTTAAAAG CAATGAGGAA CACAGCCAAA 
AAGCATATGG ATACCAATGA CAGGTATTCA AAGACCATTG ATGAGAAGGA GAAGCAGTAT 
AACACATGTT TGAATACTTA TCTTGAGACC AGTAATAAAT TTGCTAATGA AAAAGTAAAA 
TTGGAAGAAC TTATTAAAAA GAGTCAAGAT GACCTCAAGG AGCATGAGAA AAGAGCTGTT 
ACAGCAGAGA TATCAGTACT CAAAAACTGG AAAGAAAGCG AACTGTGCAA ACTAAGTGGC 
ATAGTTGCCA GAGCAGAAAC AAATCTCAAG ATGTTGAAGT CACTAAGCAG CAACTCAGCT 
TCAGCGCTTC CTGACTTAAA GTCACAGATT AATTATTGGG AAGTATTTAT TTCAAATATA 
AAGAAACAAA TGGAGAAAGT AAAGGTTGGG TACGAAGAAA AAATTGAGAA GGTGAAAAAT 
GGTGTTCGAA ACTGCCTCAC CAAAATAGAA GCTGCGGATC TTTCATCTCT TCCCAGTGTC 
TTAAGAAAGC AAAGCAGACC TGCTGTCAGT GATCCAGCCC TTGTCCTGTG TTCCTCTGCA 
TCTGCACAGC CTAATTTACT TCCTGCAGTT TCGAGTTCTG AAGATCAAGG TCCAACAACT 
GCTTCATTTA ATAGCCAGGC TGGAGTTAAG CCTGCAAATG CAAATTCTAA GGTTTGCTCT 
GCAAGTGATG GATCAGTGAA AACAAACTCC AAAGCTCGGA CAGGATCGTA TGCTGATAAA 
GTTTCGCTAA ATTCTATGCC AAAGGTTTCA GGAAGCAATA CTCAGAATAT ACAGCCAAAC 
CAAACCCACC AAGATGATTC AGCTACACAG ATGAAGCCCA CTTCAAACAA CACATTTGAA 
AATATTATTG CTCATCTACA AACTATATTT CCGGGCTACA GCAGTTCAGA GTTCACGAGG 
TTCATAAAAG ATGTACAAAG AAGAAGTGGG AATACATTAA GTTTGAGCTC CACTGAAATT 
CTAAGCAGAG TGACAGATCT CATTCTGGAC CAGCAAAATG AGGCATCAGC TTCTGTAGGG 
AGACCTATGA TGTCTGCATC ATCCGCTTCA GCGGGACAGA CGGGAACTCA AAGTCAGGAA 
GCAGCTGCAG AAGGAAATAT TTCCAGCGCA CCCAAGGCAA GGCCTGCACA CAAAAGCACC 
TCCAGTAAAA ACACGCTGCC ATCTCAGCCA AATCTCCAGC CATGGGGGAA TGCTGGAGCA 
ACACCGAAAT CTAAATGGAA AAAACTAGAC TATATACCTT CCAGCGATGA TCCGTGCACC 
ATCTGCCATG ATGAGTTAAG CAGAGACTCA TGTGAACTGG AGTGTGGGCA TCATTTTCAC 
AGAGAATGCA TTAGAAAATG GCTGAAGGAA CATTCGAGTA CCTGCCCTAT CTGTCGCATC 
CATGTTCTGT TACCTGAAGA CTTTCCTGAG CTCCCTGCAC GCAACAAATA TGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301422.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156908.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156908.1
CDS	114..6029
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301422.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301422.1

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301429.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156915.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301429.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156915.1
CDS	118..6033
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301429.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301429.1

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301416.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156902.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156902.1
CDS	139..6054
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301416.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301416.2

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301429.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156915.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156915.1
CDS	136..6051
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301429.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301429.2

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301416.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156902.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301416.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156902.1
CDS	103..6018
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301416.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301416.1

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301422.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156908.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

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CloneID	OGa30357
Clone ID Related Accession (Same CDS sequence)	XM_015301422.1 , XM_015301429.1 , XM_015301416.2 , XM_015301429.2 , XM_015301416.1 , XM_015301422.2
Accession Version	XM_015301422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156908.1
CDS	123..6038
Translation	MGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301422.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301422.2

ORF Insert Sequence:

ATGGGTGAAC CTTTGTCTGA ATCTGAGTTT GCGGCCTTGG CTTTCAGTGC TGGTTATAAA 
TCCTGCTGTG AATTGGGTCA TGGAGTATTT GTACAACGTT CTCCACATAA ACCTAAAACT 
GATGTTGACC CCTGTGAAGT GTGGTGCCGT CAGCCGGTTG ACAAACTAAG AGAATATTGC 
AACGTTATTA AAATACGCAT CTTTTGGCCT CTTCTCTTCC AAAGTGAAAG TAATTATTTG 
GTAGCTGACT GGGGCAGTTT ACAGATTTTA GACTGGAATT GGAAGAAACT CTGTGATTTA 
GAGCTTGTGG AAGACCTTGT TGATTTGACT AAAAAAGTGG TTAATGTTCC ACATTTTATT 
GGGGGCATAC TGAGAATTGG CAGCAGGTTA GAAAACGGTT TCTTCGATGT TAGGGATGCA 
CTTGATTGGA TCAGATGTAC AGGTGATATC AATATTCTGC AGAAGCTGGA GAAACTTGGT 
GATTTTGGTT GGCTCTATCT TGAAACTTTC TTTGGTGAAT GCAAACATTA TATTACTAAA 
GTTGCTTTGG AAGATTGTAA TCTAGTTGAA GAATTTAAAG CTGTCACCTG TGAAAACTGC 
AGGAAGAAGA GTGAATCTAT GAAACAGAAA GGAAATGAGG AGTTCTCCCA AGGAAAATAT 
GATCATGCTA TAATATCATA TACTAAAGCC ATAGAGTTTT GTCCTGCAAA CCATCTTCTG 
TATGGAAACC GAGCTCTTTG TTTAATTCTC ACGAAGCAGT ACAAGAGAGC CCTTGCTGAT 
GGAAAAAGGG CCACTATTTT GAAGCCTAAT TGGCCAAAGG GTCATTACCA CTTCTGTAAG 
GCGCTGTCAT TACTGGGGGA GCATGAACTG GCTTTAGAAG CTAATGAGAG AGCTCAGGAA 
CTGTGCAAGA ATATTCTTGA TGGATTTAAG GATCTTATTC AACAAAATGA CAAGTTAAAG 
AAGACATTAG AGGAAATCAA GGGTACTAAA GAATATAAGC AGAAACCAAA GAATTTGCTT 
CTTGAGAAAA AGGACTGTGG TTCTTCAAAA TCGCATTTGA TCGTTTCTCA AGAACAAAAA 
GAAATAGAGA AGGACAGAAA ACAAATGCAG TTTGATCATA AAGATGACCA TTATCATCAA 
AAACACGATA CCACCATAAG ATATCCTACA GATGGAATTC CAAGACTATC AGTGACCAAC 
ATGCCCAGGA CAGAAAACAA AGTGTGGTCT CTGGACTTTC AACCAAGCCA AAGTCACCAA 
CACAAAGGAA GGTCGAAGAA CAAGCCTGGT GAACCTGAAA AGAAACAGGA TCACCTTGAT 
TTGAAGATAG ACTCTAAAAG CATCCAAGAT AAACTCGTCT GTACTGTGCA CCAGGTGGAT 
TCAACTATGT TGCTTGAAGT GGTTAAATCC CTCGTTCATG ATGGTTACAC AGCCTTGATG 
GACCAGAGGT GTCACAGTGC TGAACAAATG TTCTCACAAC TGTTGAACAT TCTCAATCCT 
TCGGAATTAA AGCAATTGAA TCTTCGTGTT ATTAATTATG TTGTAATCAT CTATGGACAT 
GCCACTTCTC TGCTTGGAAT AGGACAGCCC GAGGCGTTGA CAAAGGCTGA AGACCAGTTT 
AAAAGAATAA TTATACAGTA CCAGCAAGAA AGATTTGATT GTCTGGCATA CTATGGAATT 
GGAAAAGTAT ACCTCAGACA AAACAGATTT TTAGATGCGC TCAATCAGTT TAAGAAGTCA 
CAAACAATGA TTAATCATAA GATTGTACCT GGAGTATTAA CTTGGCCCAC GACATCTCAG 
GTCATTGAAG AAACACGAAC AGAGAATTTA CAGTTGTTCT TGAAGAACTG CATTGAGGAA 
TGCAAGTTCC CTCCAGAACC GGATGCAATT TGTCGCTATC AGCAGTGCCA TGGCCACTCC 
AAAATCCAAA TATTTTTTAC AGATCCAGAC TTTAAGGGTT TTATACGTAT TACTTGCTGT 
CAGCAATGTA GAGTCGAGTT TCATACCAGC TGTTGGAAAA AGTTGAAAAC TGCAAACTAC 
AGTGATAAGA ATGACAAGGA TTTTCTTCAA GAATTATGTT TCACTCCCGA CTGTAGAGGC 
CTTATTTCAA AAATAGTTAT TTTCAGTTCT TCTGGTTTGG TAAAACGTGA ATTTGAACAA 
AAAATCACAA AACCCAAGGA ACCACCAAGA GCCATTATTA AACAGAAATG TTCAAGTTTT 
AGGAAGTTAA AAAGGAAAGA AGAAAAAAAA ATACGAAGAA AACGTGCACG GGAAGATGCA 
CTGAATTCTT CTAAACAGAA AATAGAAGAA AATCAGATGC GAAATGAACT GTCTCAGTAC 
CGTAATCGCA GTGATTATGC AGGTTATACT GGTGATCAAG TCCTGCAGCG TATCATTCAA 
AATGCTGAGC AAATAAAGAC TGCTGTTCGT GATGCCTCCA AACTGTTAAA GGAATTACTT 
TCCTGGTTTG TAATCAGTGA AGAGGATTAC ACATCATATT GCAAAACTAG CAGCATGTCA 
CATGCTGTGG TGGAGCAGCT CATCGGTTAC TTGATTCAGG AAGAAAACAG AGTGAAAACA 
AGGAGTTTTA TCCGTGTCCT GAGTGAAATG GAAGAAGTGG ATCCCAAATT ACAGAGGTGG 
ATGAAACACC TTAATGATTT GGGGTTGAGA GCTACAGTGA ACTTTATCCA TCAGTATGGA 
CAATTTTTAA AACAACTTGA CTTTTCTATT TTATCTGCAC CCTGGAATGA GAAGTATGGT 
AGTAAATTTG ACTACGTGTC AAGTAGTTCT GAAGGCAAAG AAATTCTTGA CTATTTTTTA 
AAACCACCTC TAGAAAAGGT CCGTTGTTTT ATATGGCTGT TAGAAGACAA CAGAGAACAT 
TTTCCATCTC TTCATCAAGC TTTAGATGAA TTCTTTGATA AAACGGACAA CCCTACCATC 
ATATTAAAGA AGCATGGGAA TGAAGATATA TTGACTAATG GTATCAAAGT TAAAAACAAA 
AGCAGAAAGA AGAACTATAA AGACTCACGG TCTGTTATAG TGTTATCCAG TGGAGTTAGT 
ACAGCACCAC AAGAAGAAAA TACATTTATA GAAGAAACTA CATTAGATTG TACGTATTAT 
GAACCATGTA TTATCCCAGA ATATCTTCGG AAGCAACTAG AGGAGTTTGA ATCCGTGTAT 
GACATTTCAA ACAGTAACAG CTATCAAAGA CTGGATGATA ATCTAAATCA AACCTGTGAA 
AGTTTATATG ACTATTTCTC TCAGATATTG GAAGAGCATG GACCGATGGA ATTAAATAAT 
AAGTTGCTGG TTGGGGAATA TGAACATTTC CCTGAAGAAG CTCGAAAGAT TGTAGAAGAT 
GAGGGTGGTT TGAAATCTTT TCTTCTGAAA TCCCATCGTT TCGTCATGGT GGATAATTTT 
ATTAGCTTAA GACATGATGT TCTTATTAAA GAAAATAAAA ACAGAGACGA GACTGAAAAT 
AATGAAGAAG GAAATTATAT TTTCTGTGAT GCGCAAGAAA ATTCTTTCCA GAACAAGATA 
CAGTTAAATC CAGCTGCTAA GGAATTCAAA CCACTGTCTT ATCCTGAACA ACCTCATCTA 
TCAACATCTA CTGACATATC ATTAGAAAGC TGTGAGACTC CACAATGTTT GCCCTACTCT 
TTGCCTACTT CATGGCAATC AATGCTTAGT CAGGATATTG ATCCTGTCGA AAATACGCCT 
TATTTTTCAA ATGTTTTATT GTATCAAGAT TTTCAGAATC AAAGCAGTTT TTTGCCTCAG 
ACTTCCTGGG GTTATCAGTG TGAAAGAACA TCGCCAGTGC CTTCTCCAGT GCCTCTCCTG 
TCAAATGTTG CCAAGCAACC TGGTTATATT TATGCTGATT GTGTCGCTCA TCAGAATAAT 
GATGAATTAG CAATTTCAGA CAGAAATTAT GTCAGCAACA GCTTTATACC AGCTGAAATA 
GAAACATACA GAGACCCTCA GATCCTGGTG GAGAACTCGG ATAACGCATT TTATGAGGAC 
AATTTTGCTG TTGCAAATAG TACAAGTGAA GCTGAATGTG ATACTCAGGT GATTAAGAAG 
GAAAAAGAAA GCAGAGGTAT ACCTGTAATG AAAAACAATC CGCACACGAG GATGGTAGCT 
GTTCAGGTGA ATAGTGAAGT AACTGATAGG GAAACAAATA CTTTGCCTTT TCATCCATTT 
GAGAAGCAAC AAGGAGATAT TCTACGTATG GAAAAAGAGC ATCAAGTATT AAAAGAGCAA 
CTTAAAGAAG CACAGGAAAA ATACGAACAA TTACAGAACC GGAGCTCAGA GGAAATCAGT 
GTTTTAGAAG AGCTGATAAA GAAGATTGTT GAAGAGAAAG GGATATTTAA GAAAGAAGTG 
GAGTGGCTTC ATCAATCTTT AGAAATAAAA GTAAAAAAAT GGCAACAGGA GAAAAAGGAA 
AATCAAGAGA ACTTAAAAGC AATGAGGAAC ACAGCCAAAA AGCATATGGA TACCAATGAC 
AGGTATTCAA AGACCATTGA TGAGAAGGAG AAGCAGTATA ACACATGTTT GAATACTTAT 
CTTGAGACCA GTAATAAATT TGCTAATGAA AAAGTAAAAT TGGAAGAACT TATTAAAAAG 
AGTCAAGATG ACCTCAAGGA GCATGAGAAA AGAGCTGTTA CAGCAGAGAT ATCAGTACTC 
AAAAACTGGA AAGAAAGCGA ACTGTGCAAA CTAAGTGGCA TAGTTGCCAG AGCAGAAACA 
AATCTCAAGA TGTTGAAGTC ACTAAGCAGC AACTCAGCTT CAGCGCTTCC TGACTTAAAG 
TCACAGATTA ATTATTGGGA AGTATTTATT TCAAATATAA AGAAACAAAT GGAGAAAGTA 
AAGGTTGGGT ACGAAGAAAA AATTGAGAAG GTGAAAAATG GTGTTCGAAA CTGCCTCACC 
AAAATAGAAG CTGCGGATCT TTCATCTCTT CCCAGTGTCT TAAGAAAGCA AAGCAGACCT 
GCTGTCAGTG ATCCAGCCCT TGTCCTGTGT TCCTCTGCAT CTGCACAGCC TAATTTACTT 
CCTGCAGTTT CGAGTTCTGA AGATCAAGGT CCAACAACTG CTTCATTTAA TAGCCAGGCT 
GGAGTTAAGC CTGCAAATGC AAATTCTAAG GTTTGCTCTG CAAGTGATGG ATCAGTGAAA 
ACAAACTCCA AAGCTCGGAC AGGATCGTAT GCTGATAAAG TTTCGCTAAA TTCTATGCCA 
AAGGTTTCAG GAAGCAATAC TCAGAATATA CAGCCAAACC AAACCCACCA AGATGATTCA 
GCTACACAGA TGAAGCCCAC TTCAAACAAC ACATTTGAAA ATATTATTGC TCATCTACAA 
ACTATATTTC CGGGCTACAG CAGTTCAGAG TTCACGAGGT TCATAAAAGA TGTACAAAGA 
AGAAGTGGGA ATACATTAAG TTTGAGCTCC ACTGAAATTC TAAGCAGAGT GACAGATCTC 
ATTCTGGACC AGCAAAATGA GGCATCAGCT TCTGTAGGGA GACCTATGAT GTCTGCATCA 
TCCGCTTCAG CGGGACAGAC GGGAACTCAA AGTCAGGAAG CAGCTGCAGA AGGAAATATT 
TCCAGCGCAC CCAAGGCAAG GCCTGCACAC AAAAGCACCT CCAGTAAAAA CACGCTGCCA 
TCTCAGCCAA ATCTCCAGCC ATGGGGGAAT GCTGGAGCAA CACCGAAATC TAAATGGAAA 
AAACTAGACT ATATACCTTC CAGCGATGAT CCGTGCACCA TCTGCCATGA TGAGTTAAGC 
AGAGACTCAT GTGAACTGGA GTGTGGGCAT CATTTTCACA GAGAATGCAT TAGAAAATGG 
CTGAAGGAAC ATTCGAGTAC CTGCCCTATC TGTCGCATCC ATGTTCTGTT ACCTGAAGAC 
TTTCCTGAGC TCCCTGCACG CAACAAATAT GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301434.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156920.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

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CloneID	OGa30358
Clone ID Related Accession (Same CDS sequence)	XM_015301434.1 , XM_015301434.2
Accession Version	XM_015301434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGT TTACAGATTT TAGACTGGAA TTGGAAGAAA 
CTCTGTGATT TAGAGCTTGT GGAAGACCTT GTTGATTTGA CTAAAAAAGT GGTTAATGTT 
CCACATTTTA TTGGGGGCAT ACTGAGAATT GGCAGCAGGT TAGAAAACGG TTTCTTCGAT 
GTTAGGGATG CACTTGATTG GATCAGATGT ACAGGTGATA TCAATATTCT GCAGAAGCTG 
GAGAAACTTG GTGATTTTGG TTGGCTCTAT CTTGAAACTT TCTTTGGTGA ATGCAAACAT 
TATATTACTA AAGTTGCTTT GGAAGATTGT AATCTAGTTG AAGAATTTAA AGCTGTCACC 
TGTGAAAACT GCAGGAAGAA GAGTGAATCT ATGAAACAGA AAGGAAATGA GGAGTTCTCC 
CAAGGAAAAT ATGATCATGC TATAATATCA TATACTAAAG CCATAGAGTT TTGTCCTGCA 
AACCATCTTC TGTATGGAAA CCGAGCTCTT TGTTTAATTC TCACGAAGCA GTACAAGAGA 
GCCCTTGCTG ATGGAAAAAG GGCCACTATT TTGAAGCCTA ATTGGCCAAA GGGTCATTAC 
CACTTCTGTA AGGCGCTGTC ATTACTGGGG GAGCATGAAC TGGCTTTAGA AGCTAATGAG 
AGAGCTCAGG AACTGTGCAA GAATATTCTT GATGGATTTA AGGATCTTAT TCAACAAAAT 
GACAAGTTAA AGAAGACATT AGAGGAAATC AAGGGTACTA AAGAATATAA GCAGAAACCA 
AAGAATTTGC TTCTTGAGAA AAAGGACTGT GGTTCTTCAA AATCGCATTT GATCGTTTCT 
CAAGAACAAA AAGAAATAGA GAAGGACAGA AAACAAATGC AGTTTGATCA TAAAGATGAC 
CATTATCATC AAAAACACGA TACCACCATA AGATATCCTA CAGATGGAAT TCCAAGACTA 
TCAGTGACCA ACATGCCCAG GACAGAAAAC AAAGTGTGGT CTCTGGACTT TCAACCAAGC 
CAAAGTCACC AACACAAAGG AAGGTCGAAG AACAAGCCTG GTGAACCTGA AAAGAAACAG 
GATCACCTTG ATTTGAAGAT AGACTCTAAA AGCATCCAAG ATAAACTCGT CTGTACTGTG 
CACCAGGTGG ATTCAACTAT GTTGCTTGAA GTGGTTAAAT CCCTCGTTCA TGATGGTTAC 
ACAGCCTTGA TGGACCAGAG GTGTCACAGT GCTGAACAAA TGTTCTCACA ACTGTTGAAC 
ATTCTCAATC CTTCGGAATT AAAGCAATTG AATCTTCGTG TTATTAATTA TGTTGTAATC 
ATCTATGGAC ATGCCACTTC TCTGCTTGGA ATAGGACAGC CCGAGGCGTT GACAAAGGCT 
GAAGACCAGT TTAAAAGAAT AATTATACAG TACCAGCAAG AAAGATTTGA TTGTCTGGCA 
TACTATGGAA TTGGAAAAGT ATACCTCAGA CAAAACAGAT TTTTAGATGC GCTCAATCAG 
TTTAAGAAGT CACAAACAAT GATTAATCAT AAGATTGTAC CTGGAGTATT AACTTGGCCC 
ACGACATCTC AGGTCATTGA AGAAACACGA ACAGAGAATT TACAGTTGTT CTTGAAGAAC 
TGCATTGAGG AATGCAAGTT CCCTCCAGAA CCGGATGCAA TTTGTCGCTA TCAGCAGTGC 
CATGGCCACT CCAAAATCCA AATATTTTTT ACAGATCCAG ACTTTAAGGG TTTTATACGT 
ATTACTTGCT GTCAGCAATG TAGAGTCGAG TTTCATACCA GCTGTTGGAA AAAGTTGAAA 
ACTGCAAACT ACAGTGATAA GAATGACAAG GATTTTCTTC AAGAATTATG TTTCACTCCC 
GACTGTAGAG GCCTTATTTC AAAAATAGTT ATTTTCAGTT CTTCTGGTTT GGTAAAACGT 
GAATTTGAAC AAAAAATCAC AAAACCCAAG GAACCACCAA GAGCCATTAT TAAACAGAAA 
TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA GAAGAAAAAA AAATACGAAG AAAACGTGCA 
CGGGAAGATG CACTGAATTC TTCTAAACAG AAAATAGAAG AAAATCAGAT GCGAAATGAA 
CTGTCTCAGT ACCGTAATCG CAGTGATTAT GCAGGTTATA CTGGTGATCA AGTCCTGCAG 
CGTATCATTC AAAATGCTGA GCAAATAAAG ACTGCTGTTC GTGATGCCTC CAAACTGTTA 
AAGGAATTAC TTTCCTGGTT TGTAATCAGT GAAGAGGATT ACACATCATA TTGCAAAACT 
AGCAGCATGT CACATGCTGT GGTGGAGCAG CTCATCGGTT ACTTGATTCA GGAAGAAAAC 
AGAGTGAAAA CAAGGAGTTT TATCCGTGTC CTGAGTGAAA TGGAAGAAGT GGATCCCAAA 
TTACAGAGGT GGATGAAACA CCTTAATGAT TTGGGGTTGA GAGCTACAGT GAACTTTATC 
CATCAGTATG GACAATTTTT AAAACAACTT GACTTTTCTA TTTTATCTGC ACCCTGGAAT 
GAGAAGTATG GTAGTAAATT TGACTACGTG TCAAGTAGTT CTGAAGGCAA AGAAATTCTT 
GACTATTTTT TAAAACCACC TCTAGAAAAG GTCCGTTGTT TTATATGGCT GTTAGAAGAC 
AACAGAGAAC ATTTTCCATC TCTTCATCAA GCTTTAGATG AATTCTTTGA TAAAACGGAC 
AACCCTACCA TCATATTAAA GAAGCATGGG AATGAAGATA TATTGACTAA TGGTATCAAA 
GTTAAAAACA AAAGCAGAAA GAAGAACTAT AAAGACTCAC GGTCTGTTAT AGTGTTATCC 
AGTGGAGTTA GTACAGCACC ACAAGAAGAA AATACATTTA TAGAAGAAAC TACATTAGAT 
TGTACGTATT ATGAACCATG TATTATCCCA GAATATCTTC GGAAGCAACT AGAGGAGTTT 
GAATCCGTGT ATGACATTTC AAACAGTAAC AGCTATCAAA GACTGGATGA TAATCTAAAT 
CAAACCTGTG AAAGTTTATA TGACTATTTC TCTCAGATAT TGGAAGAGCA TGGACCGATG 
GAATTAAATA ATAAGTTGCT GGTTGGGGAA TATGAACATT TCCCTGAAGA AGCTCGAAAG 
ATTGTAGAAG ATGAGGGTGG TTTGAAATCT TTTCTTCTGA AATCCCATCG TTTCGTCATG 
GTGGATAATT TTATTAGCTT AAGACATGAT GTTCTTATTA AAGAAAATAA AAACAGAGAC 
GAGACTGAAA ATAATGAAGA AGGAAATTAT ATTTTCTGTG ATGCGCAAGA AAATTCTTTC 
CAGAACAAGA TACAGTTAAA TCCAGCTGCT AAGGAATTCA AACCACTGTC TTATCCTGAA 
CAACCTCATC TATCAACATC TACTGACATA TCATTAGAAA GCTGTGAGAC TCCACAATGT 
TTGCCCTACT CTTTGCCTAC TTCATGGCAA TCAATGCTTA GTCAGGATAT TGATCCTGTC 
GAAAATACGC CTTATTTTTC AAATGTTTTA TTGTATCAAG ATTTTCAGAA TCAAAGCAGT 
TTTTTGCCTC AGACTTCCTG GGGTTATCAG TGTGAAAGAA CATCGCCAGT GCCTTCTCCA 
GTGCCTCTCC TGTCAAATGT TGCCAAGCAA CCTGGTTATA TTTATGCTGA TTGTGTCGCT 
CATCAGAATA ATGATGAATT AGCAATTTCA GACAGAAATT ATGTCAGCAA CAGCTTTATA 
CCAGCTGAAA TAGAAACATA CAGAGACCCT CAGATCCTGG TGGAGAACTC GGATAACGCA 
TTTTATGAGG ACAATTTTGC TGTTGCAAAT AGTACAAGTG AAGCTGAATG TGATACTCAG 
GTGATTAAGA AGGAAAAAGA AAGCAGAGGT ATACCTGTAA TGAAAAACAA TCCGCACACG 
AGGATGGTAG CTGTTCAGGT GAATAGTGAA GTAACTGATA GGGAAACAAA TACTTTGCCT 
TTTCATCCAT TTGAGAAGCA ACAAGGAGAT ATTCTACGTA TGGAAAAAGA GCATCAAGTA 
TTAAAAGAGC AACTTAAAGA AGCACAGGAA AAATACGAAC AATTACAGAA CCGGAGCTCA 
GAGGAAATCA GTGTTTTAGA AGAGCTGATA AAGAAGATTG TTGAAGAGAA AGGGATATTT 
AAGAAAGAAG TGGAGTGGCT TCATCAATCT TTAGAAATAA AAGTAAAAAA ATGGCAACAG 
GAGAAAAAGG AAAATCAAGA GAACTTAAAA GCAATGAGGA ACACAGCCAA AAAGCATATG 
GATACCAATG ACAGGTATTC AAAGACCATT GATGAGAAGG AGAAGCAGTA TAACACATGT 
TTGAATACTT ATCTTGAGAC CAGTAATAAA TTTGCTAATG AAAAAGTAAA ATTGGAAGAA 
CTTATTAAAA AGAGTCAAGA TGACCTCAAG GAGCATGAGA AAAGAGCTGT TACAGCAGAG 
ATATCAGTAC TCAAAAACTG GAAAGAAAGC GAACTGTGCA AACTAAGTGG CATAGTTGCC 
AGAGCAGAAA CAAATCTCAA GATGTTGAAG TCACTAAGCA GCAACTCAGC TTCAGCGCTT 
CCTGACTTAA AGTCACAGAT TAATTATTGG GAAGTATTTA TTTCAAATAT AAAGAAACAA 
ATGGAGAAAG TAAAGGTTGG GTACGAAGAA AAAATTGAGA AGGTGAAAAA TGGTGTTCGA 
AACTGCCTCA CCAAAATAGA AGCTGCGGAT CTTTCATCTC TTCCCAGTGT CTTAAGAAAG 
CAAAGCAGAC CTGCTGTCAG TGATCCAGCC CTTGTCCTGT GTTCCTCTGC ATCTGCACAG 
CCTAATTTAC TTCCTGCAGT TTCGAGTTCT GAAGATCAAG GTCCAACAAC TGCTTCATTT 
AATAGCCAGG CTGGAGTTAA GCCTGCAAAT GCAAATTCTA AGGTTTGCTC TGCAAGTGAT 
GGATCAGTGA AAACAAACTC CAAAGCTCGG ACAGGATCGT ATGCTGATAA AGTTTCGCTA 
AATTCTATGC CAAAGGTTTC AGGAAGCAAT ACTCAGAATA TACAGCCAAA CCAAACCCAC 
CAAGATGATT CAGCTACACA GATGAAGCCC ACTTCAAACA ACACATTTGA AAATATTATT 
GCTCATCTAC AAACTATATT TCCGGGCTAC AGCAGTTCAG AGTTCACGAG GTTCATAAAA 
GATGTACAAA GAAGAAGTGG GAATACATTA AGTTTGAGCT CCACTGAAAT TCTAAGCAGA 
GTGACAGATC TCATTCTGGA CCAGCAAAAT GAGGCATCAG CTTCTGTAGG GAGACCTATG 
ATGTCTGCAT CATCCGCTTC AGCGGGACAG ACGGGAACTC AAAGTCAGGA AGCAGCTGCA 
GAAGGAAATA TTTCCAGCGC ACCCAAGGCA AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA 
AACACGCTGC CATCTCAGCC AAATCTCCAG CCATGGGGGA ATGCTGGAGC AACACCGAAA 
TCTAAATGGA AAAAACTAGA CTATATACCT TCCAGCGATG ATCCGTGCAC CATCTGCCAT 
GATGAGTTAA GCAGAGACTC ATGTGAACTG GAGTGTGGGC ATCATTTTCA CAGAGAATGC 
ATTAGAAAAT GGCTGAAGGA ACATTCGAGT ACCTGCCCTA TCTGTCGCAT CCATGTTCTG 
TTACCTGAAG ACTTTCCTGA GCTCCCTGCA CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156920.1
CDS	100..5847
Translation	MELEPWVNLCLNLSLRPWLSVLVINPAVNCLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301434.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301434.1

ORF Insert Sequence:

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGT TTACAGATTT TAGACTGGAA TTGGAAGAAA 
CTCTGTGATT TAGAGCTTGT GGAAGACCTT GTTGATTTGA CTAAAAAAGT GGTTAATGTT 
CCACATTTTA TTGGGGGCAT ACTGAGAATT GGCAGCAGGT TAGAAAACGG TTTCTTCGAT 
GTTAGGGATG CACTTGATTG GATCAGATGT ACAGGTGATA TCAATATTCT GCAGAAGCTG 
GAGAAACTTG GTGATTTTGG TTGGCTCTAT CTTGAAACTT TCTTTGGTGA ATGCAAACAT 
TATATTACTA AAGTTGCTTT GGAAGATTGT AATCTAGTTG AAGAATTTAA AGCTGTCACC 
TGTGAAAACT GCAGGAAGAA GAGTGAATCT ATGAAACAGA AAGGAAATGA GGAGTTCTCC 
CAAGGAAAAT ATGATCATGC TATAATATCA TATACTAAAG CCATAGAGTT TTGTCCTGCA 
AACCATCTTC TGTATGGAAA CCGAGCTCTT TGTTTAATTC TCACGAAGCA GTACAAGAGA 
GCCCTTGCTG ATGGAAAAAG GGCCACTATT TTGAAGCCTA ATTGGCCAAA GGGTCATTAC 
CACTTCTGTA AGGCGCTGTC ATTACTGGGG GAGCATGAAC TGGCTTTAGA AGCTAATGAG 
AGAGCTCAGG AACTGTGCAA GAATATTCTT GATGGATTTA AGGATCTTAT TCAACAAAAT 
GACAAGTTAA AGAAGACATT AGAGGAAATC AAGGGTACTA AAGAATATAA GCAGAAACCA 
AAGAATTTGC TTCTTGAGAA AAAGGACTGT GGTTCTTCAA AATCGCATTT GATCGTTTCT 
CAAGAACAAA AAGAAATAGA GAAGGACAGA AAACAAATGC AGTTTGATCA TAAAGATGAC 
CATTATCATC AAAAACACGA TACCACCATA AGATATCCTA CAGATGGAAT TCCAAGACTA 
TCAGTGACCA ACATGCCCAG GACAGAAAAC AAAGTGTGGT CTCTGGACTT TCAACCAAGC 
CAAAGTCACC AACACAAAGG AAGGTCGAAG AACAAGCCTG GTGAACCTGA AAAGAAACAG 
GATCACCTTG ATTTGAAGAT AGACTCTAAA AGCATCCAAG ATAAACTCGT CTGTACTGTG 
CACCAGGTGG ATTCAACTAT GTTGCTTGAA GTGGTTAAAT CCCTCGTTCA TGATGGTTAC 
ACAGCCTTGA TGGACCAGAG GTGTCACAGT GCTGAACAAA TGTTCTCACA ACTGTTGAAC 
ATTCTCAATC CTTCGGAATT AAAGCAATTG AATCTTCGTG TTATTAATTA TGTTGTAATC 
ATCTATGGAC ATGCCACTTC TCTGCTTGGA ATAGGACAGC CCGAGGCGTT GACAAAGGCT 
GAAGACCAGT TTAAAAGAAT AATTATACAG TACCAGCAAG AAAGATTTGA TTGTCTGGCA 
TACTATGGAA TTGGAAAAGT ATACCTCAGA CAAAACAGAT TTTTAGATGC GCTCAATCAG 
TTTAAGAAGT CACAAACAAT GATTAATCAT AAGATTGTAC CTGGAGTATT AACTTGGCCC 
ACGACATCTC AGGTCATTGA AGAAACACGA ACAGAGAATT TACAGTTGTT CTTGAAGAAC 
TGCATTGAGG AATGCAAGTT CCCTCCAGAA CCGGATGCAA TTTGTCGCTA TCAGCAGTGC 
CATGGCCACT CCAAAATCCA AATATTTTTT ACAGATCCAG ACTTTAAGGG TTTTATACGT 
ATTACTTGCT GTCAGCAATG TAGAGTCGAG TTTCATACCA GCTGTTGGAA AAAGTTGAAA 
ACTGCAAACT ACAGTGATAA GAATGACAAG GATTTTCTTC AAGAATTATG TTTCACTCCC 
GACTGTAGAG GCCTTATTTC AAAAATAGTT ATTTTCAGTT CTTCTGGTTT GGTAAAACGT 
GAATTTGAAC AAAAAATCAC AAAACCCAAG GAACCACCAA GAGCCATTAT TAAACAGAAA 
TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA GAAGAAAAAA AAATACGAAG AAAACGTGCA 
CGGGAAGATG CACTGAATTC TTCTAAACAG AAAATAGAAG AAAATCAGAT GCGAAATGAA 
CTGTCTCAGT ACCGTAATCG CAGTGATTAT GCAGGTTATA CTGGTGATCA AGTCCTGCAG 
CGTATCATTC AAAATGCTGA GCAAATAAAG ACTGCTGTTC GTGATGCCTC CAAACTGTTA 
AAGGAATTAC TTTCCTGGTT TGTAATCAGT GAAGAGGATT ACACATCATA TTGCAAAACT 
AGCAGCATGT CACATGCTGT GGTGGAGCAG CTCATCGGTT ACTTGATTCA GGAAGAAAAC 
AGAGTGAAAA CAAGGAGTTT TATCCGTGTC CTGAGTGAAA TGGAAGAAGT GGATCCCAAA 
TTACAGAGGT GGATGAAACA CCTTAATGAT TTGGGGTTGA GAGCTACAGT GAACTTTATC 
CATCAGTATG GACAATTTTT AAAACAACTT GACTTTTCTA TTTTATCTGC ACCCTGGAAT 
GAGAAGTATG GTAGTAAATT TGACTACGTG TCAAGTAGTT CTGAAGGCAA AGAAATTCTT 
GACTATTTTT TAAAACCACC TCTAGAAAAG GTCCGTTGTT TTATATGGCT GTTAGAAGAC 
AACAGAGAAC ATTTTCCATC TCTTCATCAA GCTTTAGATG AATTCTTTGA TAAAACGGAC 
AACCCTACCA TCATATTAAA GAAGCATGGG AATGAAGATA TATTGACTAA TGGTATCAAA 
GTTAAAAACA AAAGCAGAAA GAAGAACTAT AAAGACTCAC GGTCTGTTAT AGTGTTATCC 
AGTGGAGTTA GTACAGCACC ACAAGAAGAA AATACATTTA TAGAAGAAAC TACATTAGAT 
TGTACGTATT ATGAACCATG TATTATCCCA GAATATCTTC GGAAGCAACT AGAGGAGTTT 
GAATCCGTGT ATGACATTTC AAACAGTAAC AGCTATCAAA GACTGGATGA TAATCTAAAT 
CAAACCTGTG AAAGTTTATA TGACTATTTC TCTCAGATAT TGGAAGAGCA TGGACCGATG 
GAATTAAATA ATAAGTTGCT GGTTGGGGAA TATGAACATT TCCCTGAAGA AGCTCGAAAG 
ATTGTAGAAG ATGAGGGTGG TTTGAAATCT TTTCTTCTGA AATCCCATCG TTTCGTCATG 
GTGGATAATT TTATTAGCTT AAGACATGAT GTTCTTATTA AAGAAAATAA AAACAGAGAC 
GAGACTGAAA ATAATGAAGA AGGAAATTAT ATTTTCTGTG ATGCGCAAGA AAATTCTTTC 
CAGAACAAGA TACAGTTAAA TCCAGCTGCT AAGGAATTCA AACCACTGTC TTATCCTGAA 
CAACCTCATC TATCAACATC TACTGACATA TCATTAGAAA GCTGTGAGAC TCCACAATGT 
TTGCCCTACT CTTTGCCTAC TTCATGGCAA TCAATGCTTA GTCAGGATAT TGATCCTGTC 
GAAAATACGC CTTATTTTTC AAATGTTTTA TTGTATCAAG ATTTTCAGAA TCAAAGCAGT 
TTTTTGCCTC AGACTTCCTG GGGTTATCAG TGTGAAAGAA CATCGCCAGT GCCTTCTCCA 
GTGCCTCTCC TGTCAAATGT TGCCAAGCAA CCTGGTTATA TTTATGCTGA TTGTGTCGCT 
CATCAGAATA ATGATGAATT AGCAATTTCA GACAGAAATT ATGTCAGCAA CAGCTTTATA 
CCAGCTGAAA TAGAAACATA CAGAGACCCT CAGATCCTGG TGGAGAACTC GGATAACGCA 
TTTTATGAGG ACAATTTTGC TGTTGCAAAT AGTACAAGTG AAGCTGAATG TGATACTCAG 
GTGATTAAGA AGGAAAAAGA AAGCAGAGGT ATACCTGTAA TGAAAAACAA TCCGCACACG 
AGGATGGTAG CTGTTCAGGT GAATAGTGAA GTAACTGATA GGGAAACAAA TACTTTGCCT 
TTTCATCCAT TTGAGAAGCA ACAAGGAGAT ATTCTACGTA TGGAAAAAGA GCATCAAGTA 
TTAAAAGAGC AACTTAAAGA AGCACAGGAA AAATACGAAC AATTACAGAA CCGGAGCTCA 
GAGGAAATCA GTGTTTTAGA AGAGCTGATA AAGAAGATTG TTGAAGAGAA AGGGATATTT 
AAGAAAGAAG TGGAGTGGCT TCATCAATCT TTAGAAATAA AAGTAAAAAA ATGGCAACAG 
GAGAAAAAGG AAAATCAAGA GAACTTAAAA GCAATGAGGA ACACAGCCAA AAAGCATATG 
GATACCAATG ACAGGTATTC AAAGACCATT GATGAGAAGG AGAAGCAGTA TAACACATGT 
TTGAATACTT ATCTTGAGAC CAGTAATAAA TTTGCTAATG AAAAAGTAAA ATTGGAAGAA 
CTTATTAAAA AGAGTCAAGA TGACCTCAAG GAGCATGAGA AAAGAGCTGT TACAGCAGAG 
ATATCAGTAC TCAAAAACTG GAAAGAAAGC GAACTGTGCA AACTAAGTGG CATAGTTGCC 
AGAGCAGAAA CAAATCTCAA GATGTTGAAG TCACTAAGCA GCAACTCAGC TTCAGCGCTT 
CCTGACTTAA AGTCACAGAT TAATTATTGG GAAGTATTTA TTTCAAATAT AAAGAAACAA 
ATGGAGAAAG TAAAGGTTGG GTACGAAGAA AAAATTGAGA AGGTGAAAAA TGGTGTTCGA 
AACTGCCTCA CCAAAATAGA AGCTGCGGAT CTTTCATCTC TTCCCAGTGT CTTAAGAAAG 
CAAAGCAGAC CTGCTGTCAG TGATCCAGCC CTTGTCCTGT GTTCCTCTGC ATCTGCACAG 
CCTAATTTAC TTCCTGCAGT TTCGAGTTCT GAAGATCAAG GTCCAACAAC TGCTTCATTT 
AATAGCCAGG CTGGAGTTAA GCCTGCAAAT GCAAATTCTA AGGTTTGCTC TGCAAGTGAT 
GGATCAGTGA AAACAAACTC CAAAGCTCGG ACAGGATCGT ATGCTGATAA AGTTTCGCTA 
AATTCTATGC CAAAGGTTTC AGGAAGCAAT ACTCAGAATA TACAGCCAAA CCAAACCCAC 
CAAGATGATT CAGCTACACA GATGAAGCCC ACTTCAAACA ACACATTTGA AAATATTATT 
GCTCATCTAC AAACTATATT TCCGGGCTAC AGCAGTTCAG AGTTCACGAG GTTCATAAAA 
GATGTACAAA GAAGAAGTGG GAATACATTA AGTTTGAGCT CCACTGAAAT TCTAAGCAGA 
GTGACAGATC TCATTCTGGA CCAGCAAAAT GAGGCATCAG CTTCTGTAGG GAGACCTATG 
ATGTCTGCAT CATCCGCTTC AGCGGGACAG ACGGGAACTC AAAGTCAGGA AGCAGCTGCA 
GAAGGAAATA TTTCCAGCGC ACCCAAGGCA AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA 
AACACGCTGC CATCTCAGCC AAATCTCCAG CCATGGGGGA ATGCTGGAGC AACACCGAAA 
TCTAAATGGA AAAAACTAGA CTATATACCT TCCAGCGATG ATCCGTGCAC CATCTGCCAT 
GATGAGTTAA GCAGAGACTC ATGTGAACTG GAGTGTGGGC ATCATTTTCA CAGAGAATGC 
ATTAGAAAAT GGCTGAAGGA ACATTCGAGT ACCTGCCCTA TCTGTCGCAT CCATGTTCTG 
TTACCTGAAG ACTTTCCTGA GCTCCCTGCA CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301434.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156920.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

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CloneID	OGa30358
Clone ID Related Accession (Same CDS sequence)	XM_015301434.1 , XM_015301434.2
Accession Version	XM_015301434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGT TTACAGATTT TAGACTGGAA TTGGAAGAAA 
CTCTGTGATT TAGAGCTTGT GGAAGACCTT GTTGATTTGA CTAAAAAAGT GGTTAATGTT 
CCACATTTTA TTGGGGGCAT ACTGAGAATT GGCAGCAGGT TAGAAAACGG TTTCTTCGAT 
GTTAGGGATG CACTTGATTG GATCAGATGT ACAGGTGATA TCAATATTCT GCAGAAGCTG 
GAGAAACTTG GTGATTTTGG TTGGCTCTAT CTTGAAACTT TCTTTGGTGA ATGCAAACAT 
TATATTACTA AAGTTGCTTT GGAAGATTGT AATCTAGTTG AAGAATTTAA AGCTGTCACC 
TGTGAAAACT GCAGGAAGAA GAGTGAATCT ATGAAACAGA AAGGAAATGA GGAGTTCTCC 
CAAGGAAAAT ATGATCATGC TATAATATCA TATACTAAAG CCATAGAGTT TTGTCCTGCA 
AACCATCTTC TGTATGGAAA CCGAGCTCTT TGTTTAATTC TCACGAAGCA GTACAAGAGA 
GCCCTTGCTG ATGGAAAAAG GGCCACTATT TTGAAGCCTA ATTGGCCAAA GGGTCATTAC 
CACTTCTGTA AGGCGCTGTC ATTACTGGGG GAGCATGAAC TGGCTTTAGA AGCTAATGAG 
AGAGCTCAGG AACTGTGCAA GAATATTCTT GATGGATTTA AGGATCTTAT TCAACAAAAT 
GACAAGTTAA AGAAGACATT AGAGGAAATC AAGGGTACTA AAGAATATAA GCAGAAACCA 
AAGAATTTGC TTCTTGAGAA AAAGGACTGT GGTTCTTCAA AATCGCATTT GATCGTTTCT 
CAAGAACAAA AAGAAATAGA GAAGGACAGA AAACAAATGC AGTTTGATCA TAAAGATGAC 
CATTATCATC AAAAACACGA TACCACCATA AGATATCCTA CAGATGGAAT TCCAAGACTA 
TCAGTGACCA ACATGCCCAG GACAGAAAAC AAAGTGTGGT CTCTGGACTT TCAACCAAGC 
CAAAGTCACC AACACAAAGG AAGGTCGAAG AACAAGCCTG GTGAACCTGA AAAGAAACAG 
GATCACCTTG ATTTGAAGAT AGACTCTAAA AGCATCCAAG ATAAACTCGT CTGTACTGTG 
CACCAGGTGG ATTCAACTAT GTTGCTTGAA GTGGTTAAAT CCCTCGTTCA TGATGGTTAC 
ACAGCCTTGA TGGACCAGAG GTGTCACAGT GCTGAACAAA TGTTCTCACA ACTGTTGAAC 
ATTCTCAATC CTTCGGAATT AAAGCAATTG AATCTTCGTG TTATTAATTA TGTTGTAATC 
ATCTATGGAC ATGCCACTTC TCTGCTTGGA ATAGGACAGC CCGAGGCGTT GACAAAGGCT 
GAAGACCAGT TTAAAAGAAT AATTATACAG TACCAGCAAG AAAGATTTGA TTGTCTGGCA 
TACTATGGAA TTGGAAAAGT ATACCTCAGA CAAAACAGAT TTTTAGATGC GCTCAATCAG 
TTTAAGAAGT CACAAACAAT GATTAATCAT AAGATTGTAC CTGGAGTATT AACTTGGCCC 
ACGACATCTC AGGTCATTGA AGAAACACGA ACAGAGAATT TACAGTTGTT CTTGAAGAAC 
TGCATTGAGG AATGCAAGTT CCCTCCAGAA CCGGATGCAA TTTGTCGCTA TCAGCAGTGC 
CATGGCCACT CCAAAATCCA AATATTTTTT ACAGATCCAG ACTTTAAGGG TTTTATACGT 
ATTACTTGCT GTCAGCAATG TAGAGTCGAG TTTCATACCA GCTGTTGGAA AAAGTTGAAA 
ACTGCAAACT ACAGTGATAA GAATGACAAG GATTTTCTTC AAGAATTATG TTTCACTCCC 
GACTGTAGAG GCCTTATTTC AAAAATAGTT ATTTTCAGTT CTTCTGGTTT GGTAAAACGT 
GAATTTGAAC AAAAAATCAC AAAACCCAAG GAACCACCAA GAGCCATTAT TAAACAGAAA 
TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA GAAGAAAAAA AAATACGAAG AAAACGTGCA 
CGGGAAGATG CACTGAATTC TTCTAAACAG AAAATAGAAG AAAATCAGAT GCGAAATGAA 
CTGTCTCAGT ACCGTAATCG CAGTGATTAT GCAGGTTATA CTGGTGATCA AGTCCTGCAG 
CGTATCATTC AAAATGCTGA GCAAATAAAG ACTGCTGTTC GTGATGCCTC CAAACTGTTA 
AAGGAATTAC TTTCCTGGTT TGTAATCAGT GAAGAGGATT ACACATCATA TTGCAAAACT 
AGCAGCATGT CACATGCTGT GGTGGAGCAG CTCATCGGTT ACTTGATTCA GGAAGAAAAC 
AGAGTGAAAA CAAGGAGTTT TATCCGTGTC CTGAGTGAAA TGGAAGAAGT GGATCCCAAA 
TTACAGAGGT GGATGAAACA CCTTAATGAT TTGGGGTTGA GAGCTACAGT GAACTTTATC 
CATCAGTATG GACAATTTTT AAAACAACTT GACTTTTCTA TTTTATCTGC ACCCTGGAAT 
GAGAAGTATG GTAGTAAATT TGACTACGTG TCAAGTAGTT CTGAAGGCAA AGAAATTCTT 
GACTATTTTT TAAAACCACC TCTAGAAAAG GTCCGTTGTT TTATATGGCT GTTAGAAGAC 
AACAGAGAAC ATTTTCCATC TCTTCATCAA GCTTTAGATG AATTCTTTGA TAAAACGGAC 
AACCCTACCA TCATATTAAA GAAGCATGGG AATGAAGATA TATTGACTAA TGGTATCAAA 
GTTAAAAACA AAAGCAGAAA GAAGAACTAT AAAGACTCAC GGTCTGTTAT AGTGTTATCC 
AGTGGAGTTA GTACAGCACC ACAAGAAGAA AATACATTTA TAGAAGAAAC TACATTAGAT 
TGTACGTATT ATGAACCATG TATTATCCCA GAATATCTTC GGAAGCAACT AGAGGAGTTT 
GAATCCGTGT ATGACATTTC AAACAGTAAC AGCTATCAAA GACTGGATGA TAATCTAAAT 
CAAACCTGTG AAAGTTTATA TGACTATTTC TCTCAGATAT TGGAAGAGCA TGGACCGATG 
GAATTAAATA ATAAGTTGCT GGTTGGGGAA TATGAACATT TCCCTGAAGA AGCTCGAAAG 
ATTGTAGAAG ATGAGGGTGG TTTGAAATCT TTTCTTCTGA AATCCCATCG TTTCGTCATG 
GTGGATAATT TTATTAGCTT AAGACATGAT GTTCTTATTA AAGAAAATAA AAACAGAGAC 
GAGACTGAAA ATAATGAAGA AGGAAATTAT ATTTTCTGTG ATGCGCAAGA AAATTCTTTC 
CAGAACAAGA TACAGTTAAA TCCAGCTGCT AAGGAATTCA AACCACTGTC TTATCCTGAA 
CAACCTCATC TATCAACATC TACTGACATA TCATTAGAAA GCTGTGAGAC TCCACAATGT 
TTGCCCTACT CTTTGCCTAC TTCATGGCAA TCAATGCTTA GTCAGGATAT TGATCCTGTC 
GAAAATACGC CTTATTTTTC AAATGTTTTA TTGTATCAAG ATTTTCAGAA TCAAAGCAGT 
TTTTTGCCTC AGACTTCCTG GGGTTATCAG TGTGAAAGAA CATCGCCAGT GCCTTCTCCA 
GTGCCTCTCC TGTCAAATGT TGCCAAGCAA CCTGGTTATA TTTATGCTGA TTGTGTCGCT 
CATCAGAATA ATGATGAATT AGCAATTTCA GACAGAAATT ATGTCAGCAA CAGCTTTATA 
CCAGCTGAAA TAGAAACATA CAGAGACCCT CAGATCCTGG TGGAGAACTC GGATAACGCA 
TTTTATGAGG ACAATTTTGC TGTTGCAAAT AGTACAAGTG AAGCTGAATG TGATACTCAG 
GTGATTAAGA AGGAAAAAGA AAGCAGAGGT ATACCTGTAA TGAAAAACAA TCCGCACACG 
AGGATGGTAG CTGTTCAGGT GAATAGTGAA GTAACTGATA GGGAAACAAA TACTTTGCCT 
TTTCATCCAT TTGAGAAGCA ACAAGGAGAT ATTCTACGTA TGGAAAAAGA GCATCAAGTA 
TTAAAAGAGC AACTTAAAGA AGCACAGGAA AAATACGAAC AATTACAGAA CCGGAGCTCA 
GAGGAAATCA GTGTTTTAGA AGAGCTGATA AAGAAGATTG TTGAAGAGAA AGGGATATTT 
AAGAAAGAAG TGGAGTGGCT TCATCAATCT TTAGAAATAA AAGTAAAAAA ATGGCAACAG 
GAGAAAAAGG AAAATCAAGA GAACTTAAAA GCAATGAGGA ACACAGCCAA AAAGCATATG 
GATACCAATG ACAGGTATTC AAAGACCATT GATGAGAAGG AGAAGCAGTA TAACACATGT 
TTGAATACTT ATCTTGAGAC CAGTAATAAA TTTGCTAATG AAAAAGTAAA ATTGGAAGAA 
CTTATTAAAA AGAGTCAAGA TGACCTCAAG GAGCATGAGA AAAGAGCTGT TACAGCAGAG 
ATATCAGTAC TCAAAAACTG GAAAGAAAGC GAACTGTGCA AACTAAGTGG CATAGTTGCC 
AGAGCAGAAA CAAATCTCAA GATGTTGAAG TCACTAAGCA GCAACTCAGC TTCAGCGCTT 
CCTGACTTAA AGTCACAGAT TAATTATTGG GAAGTATTTA TTTCAAATAT AAAGAAACAA 
ATGGAGAAAG TAAAGGTTGG GTACGAAGAA AAAATTGAGA AGGTGAAAAA TGGTGTTCGA 
AACTGCCTCA CCAAAATAGA AGCTGCGGAT CTTTCATCTC TTCCCAGTGT CTTAAGAAAG 
CAAAGCAGAC CTGCTGTCAG TGATCCAGCC CTTGTCCTGT GTTCCTCTGC ATCTGCACAG 
CCTAATTTAC TTCCTGCAGT TTCGAGTTCT GAAGATCAAG GTCCAACAAC TGCTTCATTT 
AATAGCCAGG CTGGAGTTAA GCCTGCAAAT GCAAATTCTA AGGTTTGCTC TGCAAGTGAT 
GGATCAGTGA AAACAAACTC CAAAGCTCGG ACAGGATCGT ATGCTGATAA AGTTTCGCTA 
AATTCTATGC CAAAGGTTTC AGGAAGCAAT ACTCAGAATA TACAGCCAAA CCAAACCCAC 
CAAGATGATT CAGCTACACA GATGAAGCCC ACTTCAAACA ACACATTTGA AAATATTATT 
GCTCATCTAC AAACTATATT TCCGGGCTAC AGCAGTTCAG AGTTCACGAG GTTCATAAAA 
GATGTACAAA GAAGAAGTGG GAATACATTA AGTTTGAGCT CCACTGAAAT TCTAAGCAGA 
GTGACAGATC TCATTCTGGA CCAGCAAAAT GAGGCATCAG CTTCTGTAGG GAGACCTATG 
ATGTCTGCAT CATCCGCTTC AGCGGGACAG ACGGGAACTC AAAGTCAGGA AGCAGCTGCA 
GAAGGAAATA TTTCCAGCGC ACCCAAGGCA AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA 
AACACGCTGC CATCTCAGCC AAATCTCCAG CCATGGGGGA ATGCTGGAGC AACACCGAAA 
TCTAAATGGA AAAAACTAGA CTATATACCT TCCAGCGATG ATCCGTGCAC CATCTGCCAT 
GATGAGTTAA GCAGAGACTC ATGTGAACTG GAGTGTGGGC ATCATTTTCA CAGAGAATGC 
ATTAGAAAAT GGCTGAAGGA ACATTCGAGT ACCTGCCCTA TCTGTCGCAT CCATGTTCTG 
TTACCTGAAG ACTTTCCTGA GCTCCCTGCA CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156920.1
CDS	109..5856
Translation	MELEPWVNLCLNLSLRPWLSVLVINPAVNCLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301434.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301434.2

ORF Insert Sequence:

ATGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC TTGGCTTTCA 
GTGCTGGTTA TAAATCCTGC TGTGAATTGT TTACAGATTT TAGACTGGAA TTGGAAGAAA 
CTCTGTGATT TAGAGCTTGT GGAAGACCTT GTTGATTTGA CTAAAAAAGT GGTTAATGTT 
CCACATTTTA TTGGGGGCAT ACTGAGAATT GGCAGCAGGT TAGAAAACGG TTTCTTCGAT 
GTTAGGGATG CACTTGATTG GATCAGATGT ACAGGTGATA TCAATATTCT GCAGAAGCTG 
GAGAAACTTG GTGATTTTGG TTGGCTCTAT CTTGAAACTT TCTTTGGTGA ATGCAAACAT 
TATATTACTA AAGTTGCTTT GGAAGATTGT AATCTAGTTG AAGAATTTAA AGCTGTCACC 
TGTGAAAACT GCAGGAAGAA GAGTGAATCT ATGAAACAGA AAGGAAATGA GGAGTTCTCC 
CAAGGAAAAT ATGATCATGC TATAATATCA TATACTAAAG CCATAGAGTT TTGTCCTGCA 
AACCATCTTC TGTATGGAAA CCGAGCTCTT TGTTTAATTC TCACGAAGCA GTACAAGAGA 
GCCCTTGCTG ATGGAAAAAG GGCCACTATT TTGAAGCCTA ATTGGCCAAA GGGTCATTAC 
CACTTCTGTA AGGCGCTGTC ATTACTGGGG GAGCATGAAC TGGCTTTAGA AGCTAATGAG 
AGAGCTCAGG AACTGTGCAA GAATATTCTT GATGGATTTA AGGATCTTAT TCAACAAAAT 
GACAAGTTAA AGAAGACATT AGAGGAAATC AAGGGTACTA AAGAATATAA GCAGAAACCA 
AAGAATTTGC TTCTTGAGAA AAAGGACTGT GGTTCTTCAA AATCGCATTT GATCGTTTCT 
CAAGAACAAA AAGAAATAGA GAAGGACAGA AAACAAATGC AGTTTGATCA TAAAGATGAC 
CATTATCATC AAAAACACGA TACCACCATA AGATATCCTA CAGATGGAAT TCCAAGACTA 
TCAGTGACCA ACATGCCCAG GACAGAAAAC AAAGTGTGGT CTCTGGACTT TCAACCAAGC 
CAAAGTCACC AACACAAAGG AAGGTCGAAG AACAAGCCTG GTGAACCTGA AAAGAAACAG 
GATCACCTTG ATTTGAAGAT AGACTCTAAA AGCATCCAAG ATAAACTCGT CTGTACTGTG 
CACCAGGTGG ATTCAACTAT GTTGCTTGAA GTGGTTAAAT CCCTCGTTCA TGATGGTTAC 
ACAGCCTTGA TGGACCAGAG GTGTCACAGT GCTGAACAAA TGTTCTCACA ACTGTTGAAC 
ATTCTCAATC CTTCGGAATT AAAGCAATTG AATCTTCGTG TTATTAATTA TGTTGTAATC 
ATCTATGGAC ATGCCACTTC TCTGCTTGGA ATAGGACAGC CCGAGGCGTT GACAAAGGCT 
GAAGACCAGT TTAAAAGAAT AATTATACAG TACCAGCAAG AAAGATTTGA TTGTCTGGCA 
TACTATGGAA TTGGAAAAGT ATACCTCAGA CAAAACAGAT TTTTAGATGC GCTCAATCAG 
TTTAAGAAGT CACAAACAAT GATTAATCAT AAGATTGTAC CTGGAGTATT AACTTGGCCC 
ACGACATCTC AGGTCATTGA AGAAACACGA ACAGAGAATT TACAGTTGTT CTTGAAGAAC 
TGCATTGAGG AATGCAAGTT CCCTCCAGAA CCGGATGCAA TTTGTCGCTA TCAGCAGTGC 
CATGGCCACT CCAAAATCCA AATATTTTTT ACAGATCCAG ACTTTAAGGG TTTTATACGT 
ATTACTTGCT GTCAGCAATG TAGAGTCGAG TTTCATACCA GCTGTTGGAA AAAGTTGAAA 
ACTGCAAACT ACAGTGATAA GAATGACAAG GATTTTCTTC AAGAATTATG TTTCACTCCC 
GACTGTAGAG GCCTTATTTC AAAAATAGTT ATTTTCAGTT CTTCTGGTTT GGTAAAACGT 
GAATTTGAAC AAAAAATCAC AAAACCCAAG GAACCACCAA GAGCCATTAT TAAACAGAAA 
TGTTCAAGTT TTAGGAAGTT AAAAAGGAAA GAAGAAAAAA AAATACGAAG AAAACGTGCA 
CGGGAAGATG CACTGAATTC TTCTAAACAG AAAATAGAAG AAAATCAGAT GCGAAATGAA 
CTGTCTCAGT ACCGTAATCG CAGTGATTAT GCAGGTTATA CTGGTGATCA AGTCCTGCAG 
CGTATCATTC AAAATGCTGA GCAAATAAAG ACTGCTGTTC GTGATGCCTC CAAACTGTTA 
AAGGAATTAC TTTCCTGGTT TGTAATCAGT GAAGAGGATT ACACATCATA TTGCAAAACT 
AGCAGCATGT CACATGCTGT GGTGGAGCAG CTCATCGGTT ACTTGATTCA GGAAGAAAAC 
AGAGTGAAAA CAAGGAGTTT TATCCGTGTC CTGAGTGAAA TGGAAGAAGT GGATCCCAAA 
TTACAGAGGT GGATGAAACA CCTTAATGAT TTGGGGTTGA GAGCTACAGT GAACTTTATC 
CATCAGTATG GACAATTTTT AAAACAACTT GACTTTTCTA TTTTATCTGC ACCCTGGAAT 
GAGAAGTATG GTAGTAAATT TGACTACGTG TCAAGTAGTT CTGAAGGCAA AGAAATTCTT 
GACTATTTTT TAAAACCACC TCTAGAAAAG GTCCGTTGTT TTATATGGCT GTTAGAAGAC 
AACAGAGAAC ATTTTCCATC TCTTCATCAA GCTTTAGATG AATTCTTTGA TAAAACGGAC 
AACCCTACCA TCATATTAAA GAAGCATGGG AATGAAGATA TATTGACTAA TGGTATCAAA 
GTTAAAAACA AAAGCAGAAA GAAGAACTAT AAAGACTCAC GGTCTGTTAT AGTGTTATCC 
AGTGGAGTTA GTACAGCACC ACAAGAAGAA AATACATTTA TAGAAGAAAC TACATTAGAT 
TGTACGTATT ATGAACCATG TATTATCCCA GAATATCTTC GGAAGCAACT AGAGGAGTTT 
GAATCCGTGT ATGACATTTC AAACAGTAAC AGCTATCAAA GACTGGATGA TAATCTAAAT 
CAAACCTGTG AAAGTTTATA TGACTATTTC TCTCAGATAT TGGAAGAGCA TGGACCGATG 
GAATTAAATA ATAAGTTGCT GGTTGGGGAA TATGAACATT TCCCTGAAGA AGCTCGAAAG 
ATTGTAGAAG ATGAGGGTGG TTTGAAATCT TTTCTTCTGA AATCCCATCG TTTCGTCATG 
GTGGATAATT TTATTAGCTT AAGACATGAT GTTCTTATTA AAGAAAATAA AAACAGAGAC 
GAGACTGAAA ATAATGAAGA AGGAAATTAT ATTTTCTGTG ATGCGCAAGA AAATTCTTTC 
CAGAACAAGA TACAGTTAAA TCCAGCTGCT AAGGAATTCA AACCACTGTC TTATCCTGAA 
CAACCTCATC TATCAACATC TACTGACATA TCATTAGAAA GCTGTGAGAC TCCACAATGT 
TTGCCCTACT CTTTGCCTAC TTCATGGCAA TCAATGCTTA GTCAGGATAT TGATCCTGTC 
GAAAATACGC CTTATTTTTC AAATGTTTTA TTGTATCAAG ATTTTCAGAA TCAAAGCAGT 
TTTTTGCCTC AGACTTCCTG GGGTTATCAG TGTGAAAGAA CATCGCCAGT GCCTTCTCCA 
GTGCCTCTCC TGTCAAATGT TGCCAAGCAA CCTGGTTATA TTTATGCTGA TTGTGTCGCT 
CATCAGAATA ATGATGAATT AGCAATTTCA GACAGAAATT ATGTCAGCAA CAGCTTTATA 
CCAGCTGAAA TAGAAACATA CAGAGACCCT CAGATCCTGG TGGAGAACTC GGATAACGCA 
TTTTATGAGG ACAATTTTGC TGTTGCAAAT AGTACAAGTG AAGCTGAATG TGATACTCAG 
GTGATTAAGA AGGAAAAAGA AAGCAGAGGT ATACCTGTAA TGAAAAACAA TCCGCACACG 
AGGATGGTAG CTGTTCAGGT GAATAGTGAA GTAACTGATA GGGAAACAAA TACTTTGCCT 
TTTCATCCAT TTGAGAAGCA ACAAGGAGAT ATTCTACGTA TGGAAAAAGA GCATCAAGTA 
TTAAAAGAGC AACTTAAAGA AGCACAGGAA AAATACGAAC AATTACAGAA CCGGAGCTCA 
GAGGAAATCA GTGTTTTAGA AGAGCTGATA AAGAAGATTG TTGAAGAGAA AGGGATATTT 
AAGAAAGAAG TGGAGTGGCT TCATCAATCT TTAGAAATAA AAGTAAAAAA ATGGCAACAG 
GAGAAAAAGG AAAATCAAGA GAACTTAAAA GCAATGAGGA ACACAGCCAA AAAGCATATG 
GATACCAATG ACAGGTATTC AAAGACCATT GATGAGAAGG AGAAGCAGTA TAACACATGT 
TTGAATACTT ATCTTGAGAC CAGTAATAAA TTTGCTAATG AAAAAGTAAA ATTGGAAGAA 
CTTATTAAAA AGAGTCAAGA TGACCTCAAG GAGCATGAGA AAAGAGCTGT TACAGCAGAG 
ATATCAGTAC TCAAAAACTG GAAAGAAAGC GAACTGTGCA AACTAAGTGG CATAGTTGCC 
AGAGCAGAAA CAAATCTCAA GATGTTGAAG TCACTAAGCA GCAACTCAGC TTCAGCGCTT 
CCTGACTTAA AGTCACAGAT TAATTATTGG GAAGTATTTA TTTCAAATAT AAAGAAACAA 
ATGGAGAAAG TAAAGGTTGG GTACGAAGAA AAAATTGAGA AGGTGAAAAA TGGTGTTCGA 
AACTGCCTCA CCAAAATAGA AGCTGCGGAT CTTTCATCTC TTCCCAGTGT CTTAAGAAAG 
CAAAGCAGAC CTGCTGTCAG TGATCCAGCC CTTGTCCTGT GTTCCTCTGC ATCTGCACAG 
CCTAATTTAC TTCCTGCAGT TTCGAGTTCT GAAGATCAAG GTCCAACAAC TGCTTCATTT 
AATAGCCAGG CTGGAGTTAA GCCTGCAAAT GCAAATTCTA AGGTTTGCTC TGCAAGTGAT 
GGATCAGTGA AAACAAACTC CAAAGCTCGG ACAGGATCGT ATGCTGATAA AGTTTCGCTA 
AATTCTATGC CAAAGGTTTC AGGAAGCAAT ACTCAGAATA TACAGCCAAA CCAAACCCAC 
CAAGATGATT CAGCTACACA GATGAAGCCC ACTTCAAACA ACACATTTGA AAATATTATT 
GCTCATCTAC AAACTATATT TCCGGGCTAC AGCAGTTCAG AGTTCACGAG GTTCATAAAA 
GATGTACAAA GAAGAAGTGG GAATACATTA AGTTTGAGCT CCACTGAAAT TCTAAGCAGA 
GTGACAGATC TCATTCTGGA CCAGCAAAAT GAGGCATCAG CTTCTGTAGG GAGACCTATG 
ATGTCTGCAT CATCCGCTTC AGCGGGACAG ACGGGAACTC AAAGTCAGGA AGCAGCTGCA 
GAAGGAAATA TTTCCAGCGC ACCCAAGGCA AGGCCTGCAC ACAAAAGCAC CTCCAGTAAA 
AACACGCTGC CATCTCAGCC AAATCTCCAG CCATGGGGGA ATGCTGGAGC AACACCGAAA 
TCTAAATGGA AAAAACTAGA CTATATACCT TCCAGCGATG ATCCGTGCAC CATCTGCCAT 
GATGAGTTAA GCAGAGACTC ATGTGAACTG GAGTGTGGGC ATCATTTTCA CAGAGAATGC 
ATTAGAAAAT GGCTGAAGGA ACATTCGAGT ACCTGCCCTA TCTGTCGCAT CCATGTTCTG 
TTACCTGAAG ACTTTCCTGA GCTCCCTGCA CGCAACAAAT ATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301440.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156926.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

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CloneID	OGa30359
Clone ID Related Accession (Same CDS sequence)	XM_015301440.1 , XM_015301440.2
Accession Version	XM_015301440.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTGT ATCTTATTTT AACAGTGCTG TCCTTTTTCG TTACGGTTTT TGTTTGTATT 
TACTATTTTT TTGAGCATGT GTGTATTTCT GTTAAATATT TCTGTTTGTT CCTAATGAGA 
GTCTCTGTGA AAAGCAGCAC TACTTGTACT AATACTTTAA AATGTTGGTG GGGCCAAAGT 
CACCAACACA AAGGAAGGTC GAAGAACAAG CCTGGTGAAC CTGAAAAGAA ACAGGATCAC 
CTTGATTTGA AGATAGACTC TAAAAGCATC CAAGATAAAC TCGTCTGTAC TGTGCACCAG 
GTGGATTCAA CTATGTTGCT TGAAGTGGTT AAATCCCTCG TTCATGATGG TTACACAGCC 
TTGATGGACC AGAGGTGTCA CAGTGCTGAA CAAATGTTCT CACAACTGTT GAACATTCTC 
AATCCTTCGG AATTAAAGCA ATTGAATCTT CGTGTTATTA ATTATGTTGT AATCATCTAT 
GGACATGCCA CTTCTCTGCT TGGAATAGGA CAGCCCGAGG CGTTGACAAA GGCTGAAGAC 
CAGTTTAAAA GAATAATTAT ACAGTACCAG CAAGAAAGAT TTGATTGTCT GGCATACTAT 
GGAATTGGAA AAGTATACCT CAGACAAAAC AGATTTTTAG ATGCGCTCAA TCAGTTTAAG 
AAGTCACAAA CAATGATTAA TCATAAGATT GTACCTGGAG TATTAACTTG GCCCACGACA 
TCTCAGGTCA TTGAAGAAAC ACGAACAGAG AATTTACAGT TGTTCTTGAA GAACTGCATT 
GAGGAATGCA AGTTCCCTCC AGAACCGGAT GCAATTTGTC GCTATCAGCA GTGCCATGGC 
CACTCCAAAA TCCAAATATT TTTTACAGAT CCAGACTTTA AGGGTTTTAT ACGTATTACT 
TGCTGTCAGC AATGTAGAGT CGAGTTTCAT ACCAGCTGTT GGAAAAAGTT GAAAACTGCA 
AACTACAGTG ATAAGAATGA CAAGGATTTT CTTCAAGAAT TATGTTTCAC TCCCGACTGT 
AGAGGCCTTA TTTCAAAAAT AGTTATTTTC AGTTCTTCTG GTTTGGTAAA ACGTGAATTT 
GAACAAAAAA TCACAAAACC CAAGGAACCA CCAAGAGCCA TTATTAAACA GAAATGTTCA 
AGTTTTAGGA AGTTAAAAAG GAAAGAAGAA AAAAAAATAC GAAGAAAACG TGCACGGGAA 
GATGCACTGA ATTCTTCTAA ACAGAAAATA GAAGAAAATC AGATGCGAAA TGAACTGTCT 
CAGTACCGTA ATCGCAGTGA TTATGCAGGT TATACTGGTG ATCAAGTCCT GCAGCGTATC 
ATTCAAAATG CTGAGCAAAT AAAGACTGCT GTTCGTGATG CCTCCAAACT GTTAAAGGAA 
TTACTTTCCT GGTTTGTAAT CAGTGAAGAG GATTACACAT CATATTGCAA AACTAGCAGC 
ATGTCACATG CTGTGGTGGA GCAGCTCATC GGTTACTTGA TTCAGGAAGA AAACAGAGTG 
AAAACAAGGA GTTTTATCCG TGTCCTGAGT GAAATGGAAG AAGTGGATCC CAAATTACAG 
AGGTGGATGA AACACCTTAA TGATTTGGGG TTGAGAGCTA CAGTGAACTT TATCCATCAG 
TATGGACAAT TTTTAAAACA ACTTGACTTT TCTATTTTAT CTGCACCCTG GAATGAGAAG 
TATGGTAGTA AATTTGACTA CGTGTCAAGT AGTTCTGAAG GCAAAGAAAT TCTTGACTAT 
TTTTTAAAAC CACCTCTAGA AAAGGTCCGT TGTTTTATAT GGCTGTTAGA AGACAACAGA 
GAACATTTTC CATCTCTTCA TCAAGCTTTA GATGAATTCT TTGATAAAAC GGACAACCCT 
ACCATCATAT TAAAGAAGCA TGGGAATGAA GATATATTGA CTAATGGTAT CAAAGTTAAA 
AACAAAAGCA GAAAGAAGAA CTATAAAGAC TCACGGTCTG TTATAGTGTT ATCCAGTGGA 
GTTAGTACAG CACCACAAGA AGAAAATACA TTTATAGAAG AAACTACATT AGATTGTACG 
TATTATGAAC CATGTATTAT CCCAGAATAT CTTCGGAAGC AACTAGAGGA GTTTGAATCC 
GTGTATGACA TTTCAAACAG TAACAGCTAT CAAAGACTGG ATGATAATCT AAATCAAACC 
TGTGAAAGTT TATATGACTA TTTCTCTCAG ATATTGGAAG AGCATGGACC GATGGAATTA 
AATAATAAGT TGCTGGTTGG GGAATATGAA CATTTCCCTG AAGAAGCTCG AAAGATTGTA 
GAAGATGAGG GTGGTTTGAA ATCTTTTCTT CTGAAATCCC ATCGTTTCGT CATGGTGGAT 
AATTTTATTA GCTTAAGACA TGATGTTCTT ATTAAAGAAA ATAAAAACAG AGACGAGACT 
GAAAATAATG AAGAAGGAAA TTATATTTTC TGTGATGCGC AAGAAAATTC TTTCCAGAAC 
AAGATACAGT TAAATCCAGC TGCTAAGGAA TTCAAACCAC TGTCTTATCC TGAACAACCT 
CATCTATCAA CATCTACTGA CATATCATTA GAAAGCTGTG AGACTCCACA ATGTTTGCCC 
TACTCTTTGC CTACTTCATG GCAATCAATG CTTAGTCAGG ATATTGATCC TGTCGAAAAT 
ACGCCTTATT TTTCAAATGT TTTATTGTAT CAAGATTTTC AGAATCAAAG CAGTTTTTTG 
CCTCAGACTT CCTGGGGTTA TCAGTGTGAA AGAACATCGC CAGTGCCTTC TCCAGTGCCT 
CTCCTGTCAA ATGTTGCCAA GCAACCTGGT TATATTTATG CTGATTGTGT CGCTCATCAG 
AATAATGATG AATTAGCAAT TTCAGACAGA AATTATGTCA GCAACAGCTT TATACCAGCT 
GAAATAGAAA CATACAGAGA CCCTCAGATC CTGGTGGAGA ACTCGGATAA CGCATTTTAT 
GAGGACAATT TTGCTGTTGC AAATAGTACA AGTGAAGCTG AATGTGATAC TCAGGTGATT 
AAGAAGGAAA AAGAAAGCAG AGGTATACCT GTAATGAAAA ACAATCCGCA CACGAGGATG 
GTAGCTGTTC AGGTGAATAG TGAAGTAACT GATAGGGAAA CAAATACTTT GCCTTTTCAT 
CCATTTGAGA AGCAACAAGG AGATATTCTA CGTATGGAAA AAGAGCATCA AGTATTAAAA 
GAGCAACTTA AAGAAGCACA GGAAAAATAC GAACAATTAC AGAACCGGAG CTCAGAGGAA 
ATCAGTGTTT TAGAAGAGCT GATAAAGAAG ATTGTTGAAG AGAAAGGGAT ATTTAAGAAA 
GAAGTGGAGT GGCTTCATCA ATCTTTAGAA ATAAAAGTAA AAAAATGGCA ACAGGAGAAA 
AAGGAAAATC AAGAGAACTT AAAAGCAATG AGGAACACAG CCAAAAAGCA TATGGATACC 
AATGACAGGT ATTCAAAGAC CATTGATGAG AAGGAGAAGC AGTATAACAC ATGTTTGAAT 
ACTTATCTTG AGACCAGTAA TAAATTTGCT AATGAAAAAG TAAAATTGGA AGAACTTATT 
AAAAAGAGTC AAGATGACCT CAAGGAGCAT GAGAAAAGAG CTGTTACAGC AGAGATATCA 
GTACTCAAAA ACTGGAAAGA AAGCGAACTG TGCAAACTAA GTGGCATAGT TGCCAGAGCA 
GAAACAAATC TCAAGATGTT GAAGTCACTA AGCAGCAACT CAGCTTCAGC GCTTCCTGAC 
TTAAAGTCAC AGATTAATTA TTGGGAAGTA TTTATTTCAA ATATAAAGAA ACAAATGGAG 
AAAGTAAAGG TTGGGTACGA AGAAAAAATT GAGAAGGTGA AAAATGGTGT TCGAAACTGC 
CTCACCAAAA TAGAAGCTGC GGATCTTTCA TCTCTTCCCA GTGTCTTAAG AAAGCAAAGC 
AGACCTGCTG TCAGTGATCC AGCCCTTGTC CTGTGTTCCT CTGCATCTGC ACAGCCTAAT 
TTACTTCCTG CAGTTTCGAG TTCTGAAGAT CAAGGTCCAA CAACTGCTTC ATTTAATAGC 
CAGGCTGGAG TTAAGCCTGC AAATGCAAAT TCTAAGGTTT GCTCTGCAAG TGATGGATCA 
GTGAAAACAA ACTCCAAAGC TCGGACAGGA TCGTATGCTG ATAAAGTTTC GCTAAATTCT 
ATGCCAAAGG TTTCAGGAAG CAATACTCAG AATATACAGC CAAACCAAAC CCACCAAGAT 
GATTCAGCTA CACAGATGAA GCCCACTTCA AACAACACAT TTGAAAATAT TATTGCTCAT 
CTACAAACTA TATTTCCGGG CTACAGCAGT TCAGAGTTCA CGAGGTTCAT AAAAGATGTA 
CAAAGAAGAA GTGGGAATAC ATTAAGTTTG AGCTCCACTG AAATTCTAAG CAGAGTGACA 
GATCTCATTC TGGACCAGCA AAATGAGGCA TCAGCTTCTG TAGGGAGACC TATGATGTCT 
GCATCATCCG CTTCAGCGGG ACAGACGGGA ACTCAAAGTC AGGAAGCAGC TGCAGAAGGA 
AATATTTCCA GCGCACCCAA GGCAAGGCCT GCACACAAAA GCACCTCCAG TAAAAACACG 
CTGCCATCTC AGCCAAATCT CCAGCCATGG GGGAATGCTG GAGCAACACC GAAATCTAAA 
TGGAAAAAAC TAGACTATAT ACCTTCCAGC GATGATCCGT GCACCATCTG CCATGATGAG 
TTAAGCAGAG ACTCATGTGA ACTGGAGTGT GGGCATCATT TTCACAGAGA ATGCATTAGA 
AAATGGCTGA AGGAACATTC GAGTACCTGC CCTATCTGTC GCATCCATGT TCTGTTACCT 
GAAGACTTTC CTGAGCTCCC TGCACGCAAC AAATATGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156926.1
CDS	545..5386
Translation	MPLYLILTVLSFFVTVFVCIYYFFEHVCISVKYFCLFLMRVSVKSSTTCTNTLKCWWGQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301440.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301440.1

ORF Insert Sequence:

ATGCCACTGT ATCTTATTTT AACAGTGCTG TCCTTTTTCG TTACGGTTTT TGTTTGTATT 
TACTATTTTT TTGAGCATGT GTGTATTTCT GTTAAATATT TCTGTTTGTT CCTAATGAGA 
GTCTCTGTGA AAAGCAGCAC TACTTGTACT AATACTTTAA AATGTTGGTG GGGCCAAAGT 
CACCAACACA AAGGAAGGTC GAAGAACAAG CCTGGTGAAC CTGAAAAGAA ACAGGATCAC 
CTTGATTTGA AGATAGACTC TAAAAGCATC CAAGATAAAC TCGTCTGTAC TGTGCACCAG 
GTGGATTCAA CTATGTTGCT TGAAGTGGTT AAATCCCTCG TTCATGATGG TTACACAGCC 
TTGATGGACC AGAGGTGTCA CAGTGCTGAA CAAATGTTCT CACAACTGTT GAACATTCTC 
AATCCTTCGG AATTAAAGCA ATTGAATCTT CGTGTTATTA ATTATGTTGT AATCATCTAT 
GGACATGCCA CTTCTCTGCT TGGAATAGGA CAGCCCGAGG CGTTGACAAA GGCTGAAGAC 
CAGTTTAAAA GAATAATTAT ACAGTACCAG CAAGAAAGAT TTGATTGTCT GGCATACTAT 
GGAATTGGAA AAGTATACCT CAGACAAAAC AGATTTTTAG ATGCGCTCAA TCAGTTTAAG 
AAGTCACAAA CAATGATTAA TCATAAGATT GTACCTGGAG TATTAACTTG GCCCACGACA 
TCTCAGGTCA TTGAAGAAAC ACGAACAGAG AATTTACAGT TGTTCTTGAA GAACTGCATT 
GAGGAATGCA AGTTCCCTCC AGAACCGGAT GCAATTTGTC GCTATCAGCA GTGCCATGGC 
CACTCCAAAA TCCAAATATT TTTTACAGAT CCAGACTTTA AGGGTTTTAT ACGTATTACT 
TGCTGTCAGC AATGTAGAGT CGAGTTTCAT ACCAGCTGTT GGAAAAAGTT GAAAACTGCA 
AACTACAGTG ATAAGAATGA CAAGGATTTT CTTCAAGAAT TATGTTTCAC TCCCGACTGT 
AGAGGCCTTA TTTCAAAAAT AGTTATTTTC AGTTCTTCTG GTTTGGTAAA ACGTGAATTT 
GAACAAAAAA TCACAAAACC CAAGGAACCA CCAAGAGCCA TTATTAAACA GAAATGTTCA 
AGTTTTAGGA AGTTAAAAAG GAAAGAAGAA AAAAAAATAC GAAGAAAACG TGCACGGGAA 
GATGCACTGA ATTCTTCTAA ACAGAAAATA GAAGAAAATC AGATGCGAAA TGAACTGTCT 
CAGTACCGTA ATCGCAGTGA TTATGCAGGT TATACTGGTG ATCAAGTCCT GCAGCGTATC 
ATTCAAAATG CTGAGCAAAT AAAGACTGCT GTTCGTGATG CCTCCAAACT GTTAAAGGAA 
TTACTTTCCT GGTTTGTAAT CAGTGAAGAG GATTACACAT CATATTGCAA AACTAGCAGC 
ATGTCACATG CTGTGGTGGA GCAGCTCATC GGTTACTTGA TTCAGGAAGA AAACAGAGTG 
AAAACAAGGA GTTTTATCCG TGTCCTGAGT GAAATGGAAG AAGTGGATCC CAAATTACAG 
AGGTGGATGA AACACCTTAA TGATTTGGGG TTGAGAGCTA CAGTGAACTT TATCCATCAG 
TATGGACAAT TTTTAAAACA ACTTGACTTT TCTATTTTAT CTGCACCCTG GAATGAGAAG 
TATGGTAGTA AATTTGACTA CGTGTCAAGT AGTTCTGAAG GCAAAGAAAT TCTTGACTAT 
TTTTTAAAAC CACCTCTAGA AAAGGTCCGT TGTTTTATAT GGCTGTTAGA AGACAACAGA 
GAACATTTTC CATCTCTTCA TCAAGCTTTA GATGAATTCT TTGATAAAAC GGACAACCCT 
ACCATCATAT TAAAGAAGCA TGGGAATGAA GATATATTGA CTAATGGTAT CAAAGTTAAA 
AACAAAAGCA GAAAGAAGAA CTATAAAGAC TCACGGTCTG TTATAGTGTT ATCCAGTGGA 
GTTAGTACAG CACCACAAGA AGAAAATACA TTTATAGAAG AAACTACATT AGATTGTACG 
TATTATGAAC CATGTATTAT CCCAGAATAT CTTCGGAAGC AACTAGAGGA GTTTGAATCC 
GTGTATGACA TTTCAAACAG TAACAGCTAT CAAAGACTGG ATGATAATCT AAATCAAACC 
TGTGAAAGTT TATATGACTA TTTCTCTCAG ATATTGGAAG AGCATGGACC GATGGAATTA 
AATAATAAGT TGCTGGTTGG GGAATATGAA CATTTCCCTG AAGAAGCTCG AAAGATTGTA 
GAAGATGAGG GTGGTTTGAA ATCTTTTCTT CTGAAATCCC ATCGTTTCGT CATGGTGGAT 
AATTTTATTA GCTTAAGACA TGATGTTCTT ATTAAAGAAA ATAAAAACAG AGACGAGACT 
GAAAATAATG AAGAAGGAAA TTATATTTTC TGTGATGCGC AAGAAAATTC TTTCCAGAAC 
AAGATACAGT TAAATCCAGC TGCTAAGGAA TTCAAACCAC TGTCTTATCC TGAACAACCT 
CATCTATCAA CATCTACTGA CATATCATTA GAAAGCTGTG AGACTCCACA ATGTTTGCCC 
TACTCTTTGC CTACTTCATG GCAATCAATG CTTAGTCAGG ATATTGATCC TGTCGAAAAT 
ACGCCTTATT TTTCAAATGT TTTATTGTAT CAAGATTTTC AGAATCAAAG CAGTTTTTTG 
CCTCAGACTT CCTGGGGTTA TCAGTGTGAA AGAACATCGC CAGTGCCTTC TCCAGTGCCT 
CTCCTGTCAA ATGTTGCCAA GCAACCTGGT TATATTTATG CTGATTGTGT CGCTCATCAG 
AATAATGATG AATTAGCAAT TTCAGACAGA AATTATGTCA GCAACAGCTT TATACCAGCT 
GAAATAGAAA CATACAGAGA CCCTCAGATC CTGGTGGAGA ACTCGGATAA CGCATTTTAT 
GAGGACAATT TTGCTGTTGC AAATAGTACA AGTGAAGCTG AATGTGATAC TCAGGTGATT 
AAGAAGGAAA AAGAAAGCAG AGGTATACCT GTAATGAAAA ACAATCCGCA CACGAGGATG 
GTAGCTGTTC AGGTGAATAG TGAAGTAACT GATAGGGAAA CAAATACTTT GCCTTTTCAT 
CCATTTGAGA AGCAACAAGG AGATATTCTA CGTATGGAAA AAGAGCATCA AGTATTAAAA 
GAGCAACTTA AAGAAGCACA GGAAAAATAC GAACAATTAC AGAACCGGAG CTCAGAGGAA 
ATCAGTGTTT TAGAAGAGCT GATAAAGAAG ATTGTTGAAG AGAAAGGGAT ATTTAAGAAA 
GAAGTGGAGT GGCTTCATCA ATCTTTAGAA ATAAAAGTAA AAAAATGGCA ACAGGAGAAA 
AAGGAAAATC AAGAGAACTT AAAAGCAATG AGGAACACAG CCAAAAAGCA TATGGATACC 
AATGACAGGT ATTCAAAGAC CATTGATGAG AAGGAGAAGC AGTATAACAC ATGTTTGAAT 
ACTTATCTTG AGACCAGTAA TAAATTTGCT AATGAAAAAG TAAAATTGGA AGAACTTATT 
AAAAAGAGTC AAGATGACCT CAAGGAGCAT GAGAAAAGAG CTGTTACAGC AGAGATATCA 
GTACTCAAAA ACTGGAAAGA AAGCGAACTG TGCAAACTAA GTGGCATAGT TGCCAGAGCA 
GAAACAAATC TCAAGATGTT GAAGTCACTA AGCAGCAACT CAGCTTCAGC GCTTCCTGAC 
TTAAAGTCAC AGATTAATTA TTGGGAAGTA TTTATTTCAA ATATAAAGAA ACAAATGGAG 
AAAGTAAAGG TTGGGTACGA AGAAAAAATT GAGAAGGTGA AAAATGGTGT TCGAAACTGC 
CTCACCAAAA TAGAAGCTGC GGATCTTTCA TCTCTTCCCA GTGTCTTAAG AAAGCAAAGC 
AGACCTGCTG TCAGTGATCC AGCCCTTGTC CTGTGTTCCT CTGCATCTGC ACAGCCTAAT 
TTACTTCCTG CAGTTTCGAG TTCTGAAGAT CAAGGTCCAA CAACTGCTTC ATTTAATAGC 
CAGGCTGGAG TTAAGCCTGC AAATGCAAAT TCTAAGGTTT GCTCTGCAAG TGATGGATCA 
GTGAAAACAA ACTCCAAAGC TCGGACAGGA TCGTATGCTG ATAAAGTTTC GCTAAATTCT 
ATGCCAAAGG TTTCAGGAAG CAATACTCAG AATATACAGC CAAACCAAAC CCACCAAGAT 
GATTCAGCTA CACAGATGAA GCCCACTTCA AACAACACAT TTGAAAATAT TATTGCTCAT 
CTACAAACTA TATTTCCGGG CTACAGCAGT TCAGAGTTCA CGAGGTTCAT AAAAGATGTA 
CAAAGAAGAA GTGGGAATAC ATTAAGTTTG AGCTCCACTG AAATTCTAAG CAGAGTGACA 
GATCTCATTC TGGACCAGCA AAATGAGGCA TCAGCTTCTG TAGGGAGACC TATGATGTCT 
GCATCATCCG CTTCAGCGGG ACAGACGGGA ACTCAAAGTC AGGAAGCAGC TGCAGAAGGA 
AATATTTCCA GCGCACCCAA GGCAAGGCCT GCACACAAAA GCACCTCCAG TAAAAACACG 
CTGCCATCTC AGCCAAATCT CCAGCCATGG GGGAATGCTG GAGCAACACC GAAATCTAAA 
TGGAAAAAAC TAGACTATAT ACCTTCCAGC GATGATCCGT GCACCATCTG CCATGATGAG 
TTAAGCAGAG ACTCATGTGA ACTGGAGTGT GGGCATCATT TTCACAGAGA ATGCATTAGA 
AAATGGCTGA AGGAACATTC GAGTACCTGC CCTATCTGTC GCATCCATGT TCTGTTACCT 
GAAGACTTTC CTGAGCTCCC TGCACGCAAC AAATATGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_015301440.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015156926.1 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

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CloneID	OGa30359
Clone ID Related Accession (Same CDS sequence)	XM_015301440.1 , XM_015301440.2
Accession Version	XM_015301440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015301440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACTGT ATCTTATTTT AACAGTGCTG TCCTTTTTCG TTACGGTTTT TGTTTGTATT 
TACTATTTTT TTGAGCATGT GTGTATTTCT GTTAAATATT TCTGTTTGTT CCTAATGAGA 
GTCTCTGTGA AAAGCAGCAC TACTTGTACT AATACTTTAA AATGTTGGTG GGGCCAAAGT 
CACCAACACA AAGGAAGGTC GAAGAACAAG CCTGGTGAAC CTGAAAAGAA ACAGGATCAC 
CTTGATTTGA AGATAGACTC TAAAAGCATC CAAGATAAAC TCGTCTGTAC TGTGCACCAG 
GTGGATTCAA CTATGTTGCT TGAAGTGGTT AAATCCCTCG TTCATGATGG TTACACAGCC 
TTGATGGACC AGAGGTGTCA CAGTGCTGAA CAAATGTTCT CACAACTGTT GAACATTCTC 
AATCCTTCGG AATTAAAGCA ATTGAATCTT CGTGTTATTA ATTATGTTGT AATCATCTAT 
GGACATGCCA CTTCTCTGCT TGGAATAGGA CAGCCCGAGG CGTTGACAAA GGCTGAAGAC 
CAGTTTAAAA GAATAATTAT ACAGTACCAG CAAGAAAGAT TTGATTGTCT GGCATACTAT 
GGAATTGGAA AAGTATACCT CAGACAAAAC AGATTTTTAG ATGCGCTCAA TCAGTTTAAG 
AAGTCACAAA CAATGATTAA TCATAAGATT GTACCTGGAG TATTAACTTG GCCCACGACA 
TCTCAGGTCA TTGAAGAAAC ACGAACAGAG AATTTACAGT TGTTCTTGAA GAACTGCATT 
GAGGAATGCA AGTTCCCTCC AGAACCGGAT GCAATTTGTC GCTATCAGCA GTGCCATGGC 
CACTCCAAAA TCCAAATATT TTTTACAGAT CCAGACTTTA AGGGTTTTAT ACGTATTACT 
TGCTGTCAGC AATGTAGAGT CGAGTTTCAT ACCAGCTGTT GGAAAAAGTT GAAAACTGCA 
AACTACAGTG ATAAGAATGA CAAGGATTTT CTTCAAGAAT TATGTTTCAC TCCCGACTGT 
AGAGGCCTTA TTTCAAAAAT AGTTATTTTC AGTTCTTCTG GTTTGGTAAA ACGTGAATTT 
GAACAAAAAA TCACAAAACC CAAGGAACCA CCAAGAGCCA TTATTAAACA GAAATGTTCA 
AGTTTTAGGA AGTTAAAAAG GAAAGAAGAA AAAAAAATAC GAAGAAAACG TGCACGGGAA 
GATGCACTGA ATTCTTCTAA ACAGAAAATA GAAGAAAATC AGATGCGAAA TGAACTGTCT 
CAGTACCGTA ATCGCAGTGA TTATGCAGGT TATACTGGTG ATCAAGTCCT GCAGCGTATC 
ATTCAAAATG CTGAGCAAAT AAAGACTGCT GTTCGTGATG CCTCCAAACT GTTAAAGGAA 
TTACTTTCCT GGTTTGTAAT CAGTGAAGAG GATTACACAT CATATTGCAA AACTAGCAGC 
ATGTCACATG CTGTGGTGGA GCAGCTCATC GGTTACTTGA TTCAGGAAGA AAACAGAGTG 
AAAACAAGGA GTTTTATCCG TGTCCTGAGT GAAATGGAAG AAGTGGATCC CAAATTACAG 
AGGTGGATGA AACACCTTAA TGATTTGGGG TTGAGAGCTA CAGTGAACTT TATCCATCAG 
TATGGACAAT TTTTAAAACA ACTTGACTTT TCTATTTTAT CTGCACCCTG GAATGAGAAG 
TATGGTAGTA AATTTGACTA CGTGTCAAGT AGTTCTGAAG GCAAAGAAAT TCTTGACTAT 
TTTTTAAAAC CACCTCTAGA AAAGGTCCGT TGTTTTATAT GGCTGTTAGA AGACAACAGA 
GAACATTTTC CATCTCTTCA TCAAGCTTTA GATGAATTCT TTGATAAAAC GGACAACCCT 
ACCATCATAT TAAAGAAGCA TGGGAATGAA GATATATTGA CTAATGGTAT CAAAGTTAAA 
AACAAAAGCA GAAAGAAGAA CTATAAAGAC TCACGGTCTG TTATAGTGTT ATCCAGTGGA 
GTTAGTACAG CACCACAAGA AGAAAATACA TTTATAGAAG AAACTACATT AGATTGTACG 
TATTATGAAC CATGTATTAT CCCAGAATAT CTTCGGAAGC AACTAGAGGA GTTTGAATCC 
GTGTATGACA TTTCAAACAG TAACAGCTAT CAAAGACTGG ATGATAATCT AAATCAAACC 
TGTGAAAGTT TATATGACTA TTTCTCTCAG ATATTGGAAG AGCATGGACC GATGGAATTA 
AATAATAAGT TGCTGGTTGG GGAATATGAA CATTTCCCTG AAGAAGCTCG AAAGATTGTA 
GAAGATGAGG GTGGTTTGAA ATCTTTTCTT CTGAAATCCC ATCGTTTCGT CATGGTGGAT 
AATTTTATTA GCTTAAGACA TGATGTTCTT ATTAAAGAAA ATAAAAACAG AGACGAGACT 
GAAAATAATG AAGAAGGAAA TTATATTTTC TGTGATGCGC AAGAAAATTC TTTCCAGAAC 
AAGATACAGT TAAATCCAGC TGCTAAGGAA TTCAAACCAC TGTCTTATCC TGAACAACCT 
CATCTATCAA CATCTACTGA CATATCATTA GAAAGCTGTG AGACTCCACA ATGTTTGCCC 
TACTCTTTGC CTACTTCATG GCAATCAATG CTTAGTCAGG ATATTGATCC TGTCGAAAAT 
ACGCCTTATT TTTCAAATGT TTTATTGTAT CAAGATTTTC AGAATCAAAG CAGTTTTTTG 
CCTCAGACTT CCTGGGGTTA TCAGTGTGAA AGAACATCGC CAGTGCCTTC TCCAGTGCCT 
CTCCTGTCAA ATGTTGCCAA GCAACCTGGT TATATTTATG CTGATTGTGT CGCTCATCAG 
AATAATGATG AATTAGCAAT TTCAGACAGA AATTATGTCA GCAACAGCTT TATACCAGCT 
GAAATAGAAA CATACAGAGA CCCTCAGATC CTGGTGGAGA ACTCGGATAA CGCATTTTAT 
GAGGACAATT TTGCTGTTGC AAATAGTACA AGTGAAGCTG AATGTGATAC TCAGGTGATT 
AAGAAGGAAA AAGAAAGCAG AGGTATACCT GTAATGAAAA ACAATCCGCA CACGAGGATG 
GTAGCTGTTC AGGTGAATAG TGAAGTAACT GATAGGGAAA CAAATACTTT GCCTTTTCAT 
CCATTTGAGA AGCAACAAGG AGATATTCTA CGTATGGAAA AAGAGCATCA AGTATTAAAA 
GAGCAACTTA AAGAAGCACA GGAAAAATAC GAACAATTAC AGAACCGGAG CTCAGAGGAA 
ATCAGTGTTT TAGAAGAGCT GATAAAGAAG ATTGTTGAAG AGAAAGGGAT ATTTAAGAAA 
GAAGTGGAGT GGCTTCATCA ATCTTTAGAA ATAAAAGTAA AAAAATGGCA ACAGGAGAAA 
AAGGAAAATC AAGAGAACTT AAAAGCAATG AGGAACACAG CCAAAAAGCA TATGGATACC 
AATGACAGGT ATTCAAAGAC CATTGATGAG AAGGAGAAGC AGTATAACAC ATGTTTGAAT 
ACTTATCTTG AGACCAGTAA TAAATTTGCT AATGAAAAAG TAAAATTGGA AGAACTTATT 
AAAAAGAGTC AAGATGACCT CAAGGAGCAT GAGAAAAGAG CTGTTACAGC AGAGATATCA 
GTACTCAAAA ACTGGAAAGA AAGCGAACTG TGCAAACTAA GTGGCATAGT TGCCAGAGCA 
GAAACAAATC TCAAGATGTT GAAGTCACTA AGCAGCAACT CAGCTTCAGC GCTTCCTGAC 
TTAAAGTCAC AGATTAATTA TTGGGAAGTA TTTATTTCAA ATATAAAGAA ACAAATGGAG 
AAAGTAAAGG TTGGGTACGA AGAAAAAATT GAGAAGGTGA AAAATGGTGT TCGAAACTGC 
CTCACCAAAA TAGAAGCTGC GGATCTTTCA TCTCTTCCCA GTGTCTTAAG AAAGCAAAGC 
AGACCTGCTG TCAGTGATCC AGCCCTTGTC CTGTGTTCCT CTGCATCTGC ACAGCCTAAT 
TTACTTCCTG CAGTTTCGAG TTCTGAAGAT CAAGGTCCAA CAACTGCTTC ATTTAATAGC 
CAGGCTGGAG TTAAGCCTGC AAATGCAAAT TCTAAGGTTT GCTCTGCAAG TGATGGATCA 
GTGAAAACAA ACTCCAAAGC TCGGACAGGA TCGTATGCTG ATAAAGTTTC GCTAAATTCT 
ATGCCAAAGG TTTCAGGAAG CAATACTCAG AATATACAGC CAAACCAAAC CCACCAAGAT 
GATTCAGCTA CACAGATGAA GCCCACTTCA AACAACACAT TTGAAAATAT TATTGCTCAT 
CTACAAACTA TATTTCCGGG CTACAGCAGT TCAGAGTTCA CGAGGTTCAT AAAAGATGTA 
CAAAGAAGAA GTGGGAATAC ATTAAGTTTG AGCTCCACTG AAATTCTAAG CAGAGTGACA 
GATCTCATTC TGGACCAGCA AAATGAGGCA TCAGCTTCTG TAGGGAGACC TATGATGTCT 
GCATCATCCG CTTCAGCGGG ACAGACGGGA ACTCAAAGTC AGGAAGCAGC TGCAGAAGGA 
AATATTTCCA GCGCACCCAA GGCAAGGCCT GCACACAAAA GCACCTCCAG TAAAAACACG 
CTGCCATCTC AGCCAAATCT CCAGCCATGG GGGAATGCTG GAGCAACACC GAAATCTAAA 
TGGAAAAAAC TAGACTATAT ACCTTCCAGC GATGATCCGT GCACCATCTG CCATGATGAG 
TTAAGCAGAG ACTCATGTGA ACTGGAGTGT GGGCATCATT TTCACAGAGA ATGCATTAGA 
AAATGGCTGA AGGAACATTC GAGTACCTGC CCTATCTGTC GCATCCATGT TCTGTTACCT 
GAAGACTTTC CTGAGCTCCC TGCACGCAAC AAATATGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015156926.1
CDS	45..4886
Translation	MPLYLILTVLSFFVTVFVCIYYFFEHVCISVKYFCLFLMRVSVKSSTTCTNTLKCWWGQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_015301440.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015301440.2

ORF Insert Sequence:

ATGCCACTGT ATCTTATTTT AACAGTGCTG TCCTTTTTCG TTACGGTTTT TGTTTGTATT 
TACTATTTTT TTGAGCATGT GTGTATTTCT GTTAAATATT TCTGTTTGTT CCTAATGAGA 
GTCTCTGTGA AAAGCAGCAC TACTTGTACT AATACTTTAA AATGTTGGTG GGGCCAAAGT 
CACCAACACA AAGGAAGGTC GAAGAACAAG CCTGGTGAAC CTGAAAAGAA ACAGGATCAC 
CTTGATTTGA AGATAGACTC TAAAAGCATC CAAGATAAAC TCGTCTGTAC TGTGCACCAG 
GTGGATTCAA CTATGTTGCT TGAAGTGGTT AAATCCCTCG TTCATGATGG TTACACAGCC 
TTGATGGACC AGAGGTGTCA CAGTGCTGAA CAAATGTTCT CACAACTGTT GAACATTCTC 
AATCCTTCGG AATTAAAGCA ATTGAATCTT CGTGTTATTA ATTATGTTGT AATCATCTAT 
GGACATGCCA CTTCTCTGCT TGGAATAGGA CAGCCCGAGG CGTTGACAAA GGCTGAAGAC 
CAGTTTAAAA GAATAATTAT ACAGTACCAG CAAGAAAGAT TTGATTGTCT GGCATACTAT 
GGAATTGGAA AAGTATACCT CAGACAAAAC AGATTTTTAG ATGCGCTCAA TCAGTTTAAG 
AAGTCACAAA CAATGATTAA TCATAAGATT GTACCTGGAG TATTAACTTG GCCCACGACA 
TCTCAGGTCA TTGAAGAAAC ACGAACAGAG AATTTACAGT TGTTCTTGAA GAACTGCATT 
GAGGAATGCA AGTTCCCTCC AGAACCGGAT GCAATTTGTC GCTATCAGCA GTGCCATGGC 
CACTCCAAAA TCCAAATATT TTTTACAGAT CCAGACTTTA AGGGTTTTAT ACGTATTACT 
TGCTGTCAGC AATGTAGAGT CGAGTTTCAT ACCAGCTGTT GGAAAAAGTT GAAAACTGCA 
AACTACAGTG ATAAGAATGA CAAGGATTTT CTTCAAGAAT TATGTTTCAC TCCCGACTGT 
AGAGGCCTTA TTTCAAAAAT AGTTATTTTC AGTTCTTCTG GTTTGGTAAA ACGTGAATTT 
GAACAAAAAA TCACAAAACC CAAGGAACCA CCAAGAGCCA TTATTAAACA GAAATGTTCA 
AGTTTTAGGA AGTTAAAAAG GAAAGAAGAA AAAAAAATAC GAAGAAAACG TGCACGGGAA 
GATGCACTGA ATTCTTCTAA ACAGAAAATA GAAGAAAATC AGATGCGAAA TGAACTGTCT 
CAGTACCGTA ATCGCAGTGA TTATGCAGGT TATACTGGTG ATCAAGTCCT GCAGCGTATC 
ATTCAAAATG CTGAGCAAAT AAAGACTGCT GTTCGTGATG CCTCCAAACT GTTAAAGGAA 
TTACTTTCCT GGTTTGTAAT CAGTGAAGAG GATTACACAT CATATTGCAA AACTAGCAGC 
ATGTCACATG CTGTGGTGGA GCAGCTCATC GGTTACTTGA TTCAGGAAGA AAACAGAGTG 
AAAACAAGGA GTTTTATCCG TGTCCTGAGT GAAATGGAAG AAGTGGATCC CAAATTACAG 
AGGTGGATGA AACACCTTAA TGATTTGGGG TTGAGAGCTA CAGTGAACTT TATCCATCAG 
TATGGACAAT TTTTAAAACA ACTTGACTTT TCTATTTTAT CTGCACCCTG GAATGAGAAG 
TATGGTAGTA AATTTGACTA CGTGTCAAGT AGTTCTGAAG GCAAAGAAAT TCTTGACTAT 
TTTTTAAAAC CACCTCTAGA AAAGGTCCGT TGTTTTATAT GGCTGTTAGA AGACAACAGA 
GAACATTTTC CATCTCTTCA TCAAGCTTTA GATGAATTCT TTGATAAAAC GGACAACCCT 
ACCATCATAT TAAAGAAGCA TGGGAATGAA GATATATTGA CTAATGGTAT CAAAGTTAAA 
AACAAAAGCA GAAAGAAGAA CTATAAAGAC TCACGGTCTG TTATAGTGTT ATCCAGTGGA 
GTTAGTACAG CACCACAAGA AGAAAATACA TTTATAGAAG AAACTACATT AGATTGTACG 
TATTATGAAC CATGTATTAT CCCAGAATAT CTTCGGAAGC AACTAGAGGA GTTTGAATCC 
GTGTATGACA TTTCAAACAG TAACAGCTAT CAAAGACTGG ATGATAATCT AAATCAAACC 
TGTGAAAGTT TATATGACTA TTTCTCTCAG ATATTGGAAG AGCATGGACC GATGGAATTA 
AATAATAAGT TGCTGGTTGG GGAATATGAA CATTTCCCTG AAGAAGCTCG AAAGATTGTA 
GAAGATGAGG GTGGTTTGAA ATCTTTTCTT CTGAAATCCC ATCGTTTCGT CATGGTGGAT 
AATTTTATTA GCTTAAGACA TGATGTTCTT ATTAAAGAAA ATAAAAACAG AGACGAGACT 
GAAAATAATG AAGAAGGAAA TTATATTTTC TGTGATGCGC AAGAAAATTC TTTCCAGAAC 
AAGATACAGT TAAATCCAGC TGCTAAGGAA TTCAAACCAC TGTCTTATCC TGAACAACCT 
CATCTATCAA CATCTACTGA CATATCATTA GAAAGCTGTG AGACTCCACA ATGTTTGCCC 
TACTCTTTGC CTACTTCATG GCAATCAATG CTTAGTCAGG ATATTGATCC TGTCGAAAAT 
ACGCCTTATT TTTCAAATGT TTTATTGTAT CAAGATTTTC AGAATCAAAG CAGTTTTTTG 
CCTCAGACTT CCTGGGGTTA TCAGTGTGAA AGAACATCGC CAGTGCCTTC TCCAGTGCCT 
CTCCTGTCAA ATGTTGCCAA GCAACCTGGT TATATTTATG CTGATTGTGT CGCTCATCAG 
AATAATGATG AATTAGCAAT TTCAGACAGA AATTATGTCA GCAACAGCTT TATACCAGCT 
GAAATAGAAA CATACAGAGA CCCTCAGATC CTGGTGGAGA ACTCGGATAA CGCATTTTAT 
GAGGACAATT TTGCTGTTGC AAATAGTACA AGTGAAGCTG AATGTGATAC TCAGGTGATT 
AAGAAGGAAA AAGAAAGCAG AGGTATACCT GTAATGAAAA ACAATCCGCA CACGAGGATG 
GTAGCTGTTC AGGTGAATAG TGAAGTAACT GATAGGGAAA CAAATACTTT GCCTTTTCAT 
CCATTTGAGA AGCAACAAGG AGATATTCTA CGTATGGAAA AAGAGCATCA AGTATTAAAA 
GAGCAACTTA AAGAAGCACA GGAAAAATAC GAACAATTAC AGAACCGGAG CTCAGAGGAA 
ATCAGTGTTT TAGAAGAGCT GATAAAGAAG ATTGTTGAAG AGAAAGGGAT ATTTAAGAAA 
GAAGTGGAGT GGCTTCATCA ATCTTTAGAA ATAAAAGTAA AAAAATGGCA ACAGGAGAAA 
AAGGAAAATC AAGAGAACTT AAAAGCAATG AGGAACACAG CCAAAAAGCA TATGGATACC 
AATGACAGGT ATTCAAAGAC CATTGATGAG AAGGAGAAGC AGTATAACAC ATGTTTGAAT 
ACTTATCTTG AGACCAGTAA TAAATTTGCT AATGAAAAAG TAAAATTGGA AGAACTTATT 
AAAAAGAGTC AAGATGACCT CAAGGAGCAT GAGAAAAGAG CTGTTACAGC AGAGATATCA 
GTACTCAAAA ACTGGAAAGA AAGCGAACTG TGCAAACTAA GTGGCATAGT TGCCAGAGCA 
GAAACAAATC TCAAGATGTT GAAGTCACTA AGCAGCAACT CAGCTTCAGC GCTTCCTGAC 
TTAAAGTCAC AGATTAATTA TTGGGAAGTA TTTATTTCAA ATATAAAGAA ACAAATGGAG 
AAAGTAAAGG TTGGGTACGA AGAAAAAATT GAGAAGGTGA AAAATGGTGT TCGAAACTGC 
CTCACCAAAA TAGAAGCTGC GGATCTTTCA TCTCTTCCCA GTGTCTTAAG AAAGCAAAGC 
AGACCTGCTG TCAGTGATCC AGCCCTTGTC CTGTGTTCCT CTGCATCTGC ACAGCCTAAT 
TTACTTCCTG CAGTTTCGAG TTCTGAAGAT CAAGGTCCAA CAACTGCTTC ATTTAATAGC 
CAGGCTGGAG TTAAGCCTGC AAATGCAAAT TCTAAGGTTT GCTCTGCAAG TGATGGATCA 
GTGAAAACAA ACTCCAAAGC TCGGACAGGA TCGTATGCTG ATAAAGTTTC GCTAAATTCT 
ATGCCAAAGG TTTCAGGAAG CAATACTCAG AATATACAGC CAAACCAAAC CCACCAAGAT 
GATTCAGCTA CACAGATGAA GCCCACTTCA AACAACACAT TTGAAAATAT TATTGCTCAT 
CTACAAACTA TATTTCCGGG CTACAGCAGT TCAGAGTTCA CGAGGTTCAT AAAAGATGTA 
CAAAGAAGAA GTGGGAATAC ATTAAGTTTG AGCTCCACTG AAATTCTAAG CAGAGTGACA 
GATCTCATTC TGGACCAGCA AAATGAGGCA TCAGCTTCTG TAGGGAGACC TATGATGTCT 
GCATCATCCG CTTCAGCGGG ACAGACGGGA ACTCAAAGTC AGGAAGCAGC TGCAGAAGGA 
AATATTTCCA GCGCACCCAA GGCAAGGCCT GCACACAAAA GCACCTCCAG TAAAAACACG 
CTGCCATCTC AGCCAAATCT CCAGCCATGG GGGAATGCTG GAGCAACACC GAAATCTAAA 
TGGAAAAAAC TAGACTATAT ACCTTCCAGC GATGATCCGT GCACCATCTG CCATGATGAG 
TTAAGCAGAG ACTCATGTGA ACTGGAGTGT GGGCATCATT TTCACAGAGA ATGCATTAGA 
AAATGGCTGA AGGAACATTC GAGTACCTGC CCTATCTGTC GCATCCATGT TCTGTTACCT 
GAAGACTTTC CTGAGCTCCC TGCACGCAAC AAATATGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_416727.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416727.4 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OGa02730
Clone ID Related Accession (Same CDS sequence)	XM_416727.5 , XM_416727.6
Accession Version	XM_416727.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455738.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416727.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGCA GTTTACAGAT TTTAGACTGG 
AATTGGAAGA AACTCTGTGA TTTAGAGCTT GTGGAAGACC TTGTTGATTT GACTAAAAAA 
GTGGTTAATG TTCCACATTT TATTGGGGGC ATACTGAGAA TTGGCAGCAG GTTAGAAAAC 
GGTTTCTTCG ATGTTAGGGA TGCACTTGAT TGGATCAGAT GTACAGGTGA TATCAATATT 
CTGCAGAAGC TGGAGAAACT TGGTGATTTT GGTTGGCTCT ATCTTGAAAC TTTCTTTGGT 
GAATGCAAAC ATTATATTAC TAAAGTTGCT TTGGAAGATT GTAATCTAGT TGAAGAATTT 
AAAGCTGTCA CCTGTGAAAA CTGCAGGAAG AAGAGTGAAT CTATGAAACA GAAAGGAAAT 
GAGGAGTTCT CCCAAGGAAA ATATGATCAT GCTATAATAT CATATACTAA AGCCATAGAG 
TTTTGTCCTG CAAACCATCT TCTGTATGGA AACCGAGCTC TTTGTTTAAT TCTCACGAAG 
CAGTACAAGA GAGCCCTTGC TGATGGAAAA AGGGCCACTA TTTTGAAGCC TAATTGGCCA 
AAGGGTCATT ACCACTTCTG TAAGGCGCTG TCATTACTGG GGGAGCATGA ACTGGCTTTA 
GAAGCTAATG AGAGAGCTCA GGAACTGTGC AAGAATATTC TTGATGGATT TAAGGATCTT 
ATTCAACAAA ATGACAAGTT AAAGAAGACA TTAGAGGAAA TCAAGGGTAC TAAAGAATAT 
AAGCAGAAAC CAAAGAATTT GCTTCTTGAG AAAAAGGACT GTGGTTCTTC AAAATCGCAT 
TTGATCGTTT CTCAAGAACA AAAAGAAATA GAGAAGGACA GAAAACAAAT GCAGTTTGAT 
CATAAAGATG ACCATTATCA TCAAAAACAC GATACCACCA TAAGATATCC TACAGATGGA 
ATTCCAAGAC TATCAGTGAC CAACATGCCC AGGACAGAAA ACAAAGTGTG GTCTCTGGAC 
TTTCAACCAA GCCAAAGTCA CCAACACAAA GGAAGGTCGA AGAACAAGCC TGGTGAACCT 
GAAAAGAAAC AGGATCACCT TGATTTGAAG ATAGACTCTA AAAGCATCCA AGATAAACTC 
GTCTGTACTG TGCACCAGGT GGATTCAACT ATGTTGCTTG AAGTGGTTAA ATCCCTCGTT 
CATGATGGTT ACACAGCCTT GATGGACCAG AGGTGTCACA GTGCTGAACA AATGTTCTCA 
CAACTGTTGA ACATTCTCAA TCCTTCGGAA TTAAAGCAAT TGAATCTTCG TGTTATTAAT 
TATGTTGTAA TCATCTATGG ACATGCCACT TCTCTGCTTG GAATAGGACA GCCCGAGGCG 
TTGACAAAGG CTGAAGACCA GTTTAAAAGA ATAATTATAC AGTACCAGCA AGAAAGATTT 
GATTGTCTGG CATACTATGG AATTGGAAAA GTATACCTCA GACAAAACAG ATTTTTAGAT 
GCGCTCAATC AGTTTAAGAA GTCACAAACA ATGATTAATC ATAAGATTGT ACCTGGAGTA 
TTAACTTGGC CCACGACATC TCAGGTCATT GAAGAAACAC GAACAGAGAA TTTACAGTTG 
TTCTTGAAGA ACTGCATTGA GGAATGCAAG TTCCCTCCAG AACCGGATGC AATTTGTCGC 
TATCAGCAGT GCCATGGCCA CTCCAAAATC CAAATATTTT TTACAGATCC AGACTTTAAG 
GGTTTTATAC GTATTACTTG CTGTCAGCAA TGTAGAGTCG AGTTTCATAC CAGCTGTTGG 
AAAAAGTTGA AAACTGCAAA CTACAGTGAT AAGAATGACA AGGATTTTCT TCAAGAATTA 
TGTTTCACTC CCGACTGTAG AGGCCTTATT TCAAAAATAG TTATTTTCAG TTCTTCTGGT 
TTGGTAAAAC GTGAATTTGA ACAAAAAATC ACAAAACCCA AGGAACCACC AAGAGCCATT 
ATTAAACAGA AATGTTCAAG TTTTAGGAAG TTAAAAAGGA AAGAAGAAAA AAAAATACGA 
AGAAAACGTG CACGGGAAGA TGCACTGAAT TCTTCTAAAC AGAAAATAGA AGAAAATCAG 
ATGCGAAATG AACTGTCTCA GTACCGTAAT CGCAGTGATT ATGCAGGTTA TACTGGTGAT 
CAAGTCCTGC AGCGTATCAT TCAAAATGCT GAGCAAATAA AGACTGCTGT TCGTGATGCC 
TCCAAACTGT TAAAGGAATT ACTTTCCTGG TTTGTAATCA GTGAAGAGGA TTACACATCA 
TATTGCAAAA CTAGCAGCAT GTCACATGCT GTGGTGGAGC AGCTCATCGG TTACTTGATT 
CAGGAAGAAA ACAGAGTGAA AACAAGGAGT TTTATCCGTG TCCTGAGTGA AATGGAAGAA 
GTGGATCCCA AATTACAGAG GTGGATGAAA CACCTTAATG ATTTGGGGTT GAGAGCTACA 
GTGAACTTTA TCCATCAGTA TGGACAATTT TTAAAACAAC TTGACTTTTC TATTTTATCT 
GCACCCTGGA ATGAGAAGTA TGGTAGTAAA TTTGACTACG TGTCAAGTAG TTCTGAAGGC 
AAAGAAATTC TTGACTATTT TTTAAAACCA CCTCTAGAAA AGGTCCGTTG TTTTATATGG 
CTGTTAGAAG ACAACAGAGA ACATTTTCCA TCTCTTCATC AAGCTTTAGA TGAATTCTTT 
GATAAAACGG ACAACCCTAC CATCATATTA AAGAAGCATG GGAATGAAGA TATATTGACT 
AATGGTATCA AAGTTAAAAA CAAAAGCAGA AAGAAGAACT ATAAAGACTC ACGGTCTGTT 
ATAGTGTTAT CCAGTGGAGT TAGTACAGCA CCACAAGAAG AAAATACATT TATAGAAGAA 
ACTACATTAG ATTGTACGTA TTATGAACCA TGTATTATCC CAGAATATCT TCGGAAGCAA 
CTAGAGGAGT TTGAATCCGT GTATGACATT TCAAACAGTA ACAGCTATCA AAGACTGGAT 
GATAATCTAA ATCAAACCTG TGAAAGTTTA TATGACTATT TCTCTCAGAT ATTGGAAGAG 
CATGGACCGA TGGAATTAAA TAATAAGTTG CTGGTTGGGG AATATGAACA TTTCCCTGAA 
GAAGCTCGAA AGATTGTAGA AGATGAGGGT GGTTTGAAAT CTTTTCTTCT GAAATCCCAT 
CGTTTCGTCA TGGTGGATAA TTTTATTAGC TTAAGACATG ATGTTCTTAT TAAAGAAAAT 
AAAAACAGAG ACGAGACTGA AAATAATGAA GAAGGAAATT ATATTTTCTG TGATGCGCAA 
GAAAATTCTT TCCAGAACAA GATACAGTTA AATCCAGCTG CTAAGGAATT CAAACCACTG 
TCTTATCCTG AACAACCTCA TCTATCAACA TCTACTGACA TATCATTAGA AAGCTGTGAG 
ACTCCACAAT GTTTGCCCTA CTCTTTGCCT ACTTCATGGC AATCAATGCT TAGTCAGGAT 
ATTGATCCTG TCGAAAATAC GCCTTATTTT TCAAATGTTT TATTGTATCA AGATTTTCAG 
AATCAAAGCA GTTTTTTGCC TCAGACTTCC TGGGGTTATC AGTGTGAAAG AACATCGCCA 
GTGCCTTCTC CAGTGCCTCT CCTGTCAAAT GTTGCCAAGC AACCTGGTTA TATTTATGCT 
GATTGTGTCG CTCATCAGAA TAATGATGAA TTAGCAATTT CAGACAGAAA TTATGTCAGC 
AACAGCTTTA TACCAGCTGA AATAGAAACA TACAGAGACC CTCAGATCCT GGTGGAGAAC 
TCGGATAACG CATTTTATGA GGACAATTTT GCTGTTGCAA ATAGTACAAG TGAAGCTGAA 
TGTGATACTC AGGTGATTAA GAAGGAAAAA GAAAGCAGAG GTATACCTGT AATGAAAAAC 
AATCCGCACA CGAGGATGGT AGCTGTTCAG GTGAATAGTG AAGTAACTGA TAGGGAAACA 
AATACTTTGC CTTTTCATCC ATTTGAGAAG CAACAAGGAG ATATTCTACG TATGGAAAAA 
GAGCATCAAG TATTAAAAGA GCAACTTAAA GAAGCACAGG AAAAATACGA ACAATTACAG 
AACCGGAGCT CAGAGGAAAT CAGTGTTTTA GAAGAGCTGA TAAAGAAGAT TGTTGAAGAG 
AAAGGGATAT TTAAGAAAGA AGTGGAGTGG CTTCATCAAT CTTTAGAAAT AAAAGTAAAA 
AAATGGCAAC AGGAGAAAAA GGAAAATCAA GAGAACTTAA AAGCAATGAG GAACACAGCC 
AAAAAGCATA TGGATACCAA TGACAGGTAT TCAAAGACCA TTGATGAGAA GGAGAAGCAG 
TATAACACAT GTTTGAATAC TTATCTTGAG ACCAGTAATA AATTTGCTAA TGAAAAAGTA 
AAATTGGAAG AACTTATTAA AAAGAGTCAA GATGACCTCA AGGAGCATGA GAAAAGAGCT 
GTTACAGCAG AGATATCAGT ACTCAAAAAC TGGAAAGAAA GCGAACTGTG CAAACTAAGT 
GGCATAGTTG CCAGAGCAGA AACAAATCTC AAGATGTTGA AGTCACTAAG CAGCAACTCA 
GCTTCAGCGC TTCCTGACTT AAAGTCACAG ATTAATTATT GGGAAGTATT TATTTCAAAT 
ATAAAGAAAC AAATGGAGAA AGTAAAGGTT GGGTACGAAG AAAAAATTGA GAAGGTGAAA 
AATGGTGTTC GAAACTGCCT CACCAAAATA GAAGCTGCGG ATCTTTCATC TCTTCCCAGT 
GTCTTAAGAA AGCAAAGCAG ACCTGCTGTC AGTGATCCAG CCCTTGTCCT GTGTTCCTCT 
GCATCTGCAC AGCCTAATTT ACTTCCTGCA GTTTCGAGTT CTGAAGATCA AGGTCCAACA 
ACTGCTTCAT TTAATAGCCA GGCTGGAGTT AAGCCTGCAA ATGCAAATTC TAAGGTTTGC 
TCTGCAAGTG ATGGATCAGT GAAAACAAAC TCCAAAGCTC GGACAGGATC GTATGCTGAT 
AAAGTTTCGC TAAATTCTAT GCCAAAGGTT TCAGGAAGCA ATACTCAGAA TATACAGCCA 
AACCAAACCC ACCAAGATGA TTCAGCTACA CAGATGAAGC CCACTTCAAA CAACACATTT 
GAAAATATTA TTGCTCATCT ACAAACTATA TTTCCGGGCT ACAGCAGTTC AGAGTTCACG 
AGGTTCATAA AAGATGTACA AAGAAGAAGT GGGAATACAT TAAGTTTGAG CTCCACTGAA 
ATTCTAAGCA GAGTGACAGA TCTCATTCTG GACCAGCAAA ATGAGGCATC AGCTTCTGTA 
GGGAGACCTA TGATGTCTGC ATCATCCGCT TCAGCGGGAC AGACGGGAAC TCAAAGTCAG 
GAAGCAGCTG CAGAAGGAAA TATTTCCAGC GCACCCAAGG CAAGGCCTGC ACACAAAAGC 
ACCTCCAGTA AAAACACGCT GCCATCTCAG CCAAATCTCC AGCCATGGGG GAATGCTGGA 
GCAACACCGA AATCTAAATG GAAAAAACTA GACTATATAC CTTCCAGCGA TGATCCGTGC 
ACCATCTGCC ATGATGAGTT AAGCAGAGAC TCATGTGAAC TGGAGTGTGG GCATCATTTT 
CACAGAGAAT GCATTAGAAA ATGGCTGAAG GAACATTCGA GTACCTGCCC TATCTGTCGC 
ATCCATGTTC TGTTACCTGA AGACTTTCCT GAGCTCCCTG CACGCAACAA ATATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416727.4
CDS	99..6038
Translation	MVGAGARTMGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_416727.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416727.5

ORF Insert Sequence:

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGCA GTTTACAGAT TTTAGACTGG 
AATTGGAAGA AACTCTGTGA TTTAGAGCTT GTGGAAGACC TTGTTGATTT GACTAAAAAA 
GTGGTTAATG TTCCACATTT TATTGGGGGC ATACTGAGAA TTGGCAGCAG GTTAGAAAAC 
GGTTTCTTCG ATGTTAGGGA TGCACTTGAT TGGATCAGAT GTACAGGTGA TATCAATATT 
CTGCAGAAGC TGGAGAAACT TGGTGATTTT GGTTGGCTCT ATCTTGAAAC TTTCTTTGGT 
GAATGCAAAC ATTATATTAC TAAAGTTGCT TTGGAAGATT GTAATCTAGT TGAAGAATTT 
AAAGCTGTCA CCTGTGAAAA CTGCAGGAAG AAGAGTGAAT CTATGAAACA GAAAGGAAAT 
GAGGAGTTCT CCCAAGGAAA ATATGATCAT GCTATAATAT CATATACTAA AGCCATAGAG 
TTTTGTCCTG CAAACCATCT TCTGTATGGA AACCGAGCTC TTTGTTTAAT TCTCACGAAG 
CAGTACAAGA GAGCCCTTGC TGATGGAAAA AGGGCCACTA TTTTGAAGCC TAATTGGCCA 
AAGGGTCATT ACCACTTCTG TAAGGCGCTG TCATTACTGG GGGAGCATGA ACTGGCTTTA 
GAAGCTAATG AGAGAGCTCA GGAACTGTGC AAGAATATTC TTGATGGATT TAAGGATCTT 
ATTCAACAAA ATGACAAGTT AAAGAAGACA TTAGAGGAAA TCAAGGGTAC TAAAGAATAT 
AAGCAGAAAC CAAAGAATTT GCTTCTTGAG AAAAAGGACT GTGGTTCTTC AAAATCGCAT 
TTGATCGTTT CTCAAGAACA AAAAGAAATA GAGAAGGACA GAAAACAAAT GCAGTTTGAT 
CATAAAGATG ACCATTATCA TCAAAAACAC GATACCACCA TAAGATATCC TACAGATGGA 
ATTCCAAGAC TATCAGTGAC CAACATGCCC AGGACAGAAA ACAAAGTGTG GTCTCTGGAC 
TTTCAACCAA GCCAAAGTCA CCAACACAAA GGAAGGTCGA AGAACAAGCC TGGTGAACCT 
GAAAAGAAAC AGGATCACCT TGATTTGAAG ATAGACTCTA AAAGCATCCA AGATAAACTC 
GTCTGTACTG TGCACCAGGT GGATTCAACT ATGTTGCTTG AAGTGGTTAA ATCCCTCGTT 
CATGATGGTT ACACAGCCTT GATGGACCAG AGGTGTCACA GTGCTGAACA AATGTTCTCA 
CAACTGTTGA ACATTCTCAA TCCTTCGGAA TTAAAGCAAT TGAATCTTCG TGTTATTAAT 
TATGTTGTAA TCATCTATGG ACATGCCACT TCTCTGCTTG GAATAGGACA GCCCGAGGCG 
TTGACAAAGG CTGAAGACCA GTTTAAAAGA ATAATTATAC AGTACCAGCA AGAAAGATTT 
GATTGTCTGG CATACTATGG AATTGGAAAA GTATACCTCA GACAAAACAG ATTTTTAGAT 
GCGCTCAATC AGTTTAAGAA GTCACAAACA ATGATTAATC ATAAGATTGT ACCTGGAGTA 
TTAACTTGGC CCACGACATC TCAGGTCATT GAAGAAACAC GAACAGAGAA TTTACAGTTG 
TTCTTGAAGA ACTGCATTGA GGAATGCAAG TTCCCTCCAG AACCGGATGC AATTTGTCGC 
TATCAGCAGT GCCATGGCCA CTCCAAAATC CAAATATTTT TTACAGATCC AGACTTTAAG 
GGTTTTATAC GTATTACTTG CTGTCAGCAA TGTAGAGTCG AGTTTCATAC CAGCTGTTGG 
AAAAAGTTGA AAACTGCAAA CTACAGTGAT AAGAATGACA AGGATTTTCT TCAAGAATTA 
TGTTTCACTC CCGACTGTAG AGGCCTTATT TCAAAAATAG TTATTTTCAG TTCTTCTGGT 
TTGGTAAAAC GTGAATTTGA ACAAAAAATC ACAAAACCCA AGGAACCACC AAGAGCCATT 
ATTAAACAGA AATGTTCAAG TTTTAGGAAG TTAAAAAGGA AAGAAGAAAA AAAAATACGA 
AGAAAACGTG CACGGGAAGA TGCACTGAAT TCTTCTAAAC AGAAAATAGA AGAAAATCAG 
ATGCGAAATG AACTGTCTCA GTACCGTAAT CGCAGTGATT ATGCAGGTTA TACTGGTGAT 
CAAGTCCTGC AGCGTATCAT TCAAAATGCT GAGCAAATAA AGACTGCTGT TCGTGATGCC 
TCCAAACTGT TAAAGGAATT ACTTTCCTGG TTTGTAATCA GTGAAGAGGA TTACACATCA 
TATTGCAAAA CTAGCAGCAT GTCACATGCT GTGGTGGAGC AGCTCATCGG TTACTTGATT 
CAGGAAGAAA ACAGAGTGAA AACAAGGAGT TTTATCCGTG TCCTGAGTGA AATGGAAGAA 
GTGGATCCCA AATTACAGAG GTGGATGAAA CACCTTAATG ATTTGGGGTT GAGAGCTACA 
GTGAACTTTA TCCATCAGTA TGGACAATTT TTAAAACAAC TTGACTTTTC TATTTTATCT 
GCACCCTGGA ATGAGAAGTA TGGTAGTAAA TTTGACTACG TGTCAAGTAG TTCTGAAGGC 
AAAGAAATTC TTGACTATTT TTTAAAACCA CCTCTAGAAA AGGTCCGTTG TTTTATATGG 
CTGTTAGAAG ACAACAGAGA ACATTTTCCA TCTCTTCATC AAGCTTTAGA TGAATTCTTT 
GATAAAACGG ACAACCCTAC CATCATATTA AAGAAGCATG GGAATGAAGA TATATTGACT 
AATGGTATCA AAGTTAAAAA CAAAAGCAGA AAGAAGAACT ATAAAGACTC ACGGTCTGTT 
ATAGTGTTAT CCAGTGGAGT TAGTACAGCA CCACAAGAAG AAAATACATT TATAGAAGAA 
ACTACATTAG ATTGTACGTA TTATGAACCA TGTATTATCC CAGAATATCT TCGGAAGCAA 
CTAGAGGAGT TTGAATCCGT GTATGACATT TCAAACAGTA ACAGCTATCA AAGACTGGAT 
GATAATCTAA ATCAAACCTG TGAAAGTTTA TATGACTATT TCTCTCAGAT ATTGGAAGAG 
CATGGACCGA TGGAATTAAA TAATAAGTTG CTGGTTGGGG AATATGAACA TTTCCCTGAA 
GAAGCTCGAA AGATTGTAGA AGATGAGGGT GGTTTGAAAT CTTTTCTTCT GAAATCCCAT 
CGTTTCGTCA TGGTGGATAA TTTTATTAGC TTAAGACATG ATGTTCTTAT TAAAGAAAAT 
AAAAACAGAG ACGAGACTGA AAATAATGAA GAAGGAAATT ATATTTTCTG TGATGCGCAA 
GAAAATTCTT TCCAGAACAA GATACAGTTA AATCCAGCTG CTAAGGAATT CAAACCACTG 
TCTTATCCTG AACAACCTCA TCTATCAACA TCTACTGACA TATCATTAGA AAGCTGTGAG 
ACTCCACAAT GTTTGCCCTA CTCTTTGCCT ACTTCATGGC AATCAATGCT TAGTCAGGAT 
ATTGATCCTG TCGAAAATAC GCCTTATTTT TCAAATGTTT TATTGTATCA AGATTTTCAG 
AATCAAAGCA GTTTTTTGCC TCAGACTTCC TGGGGTTATC AGTGTGAAAG AACATCGCCA 
GTGCCTTCTC CAGTGCCTCT CCTGTCAAAT GTTGCCAAGC AACCTGGTTA TATTTATGCT 
GATTGTGTCG CTCATCAGAA TAATGATGAA TTAGCAATTT CAGACAGAAA TTATGTCAGC 
AACAGCTTTA TACCAGCTGA AATAGAAACA TACAGAGACC CTCAGATCCT GGTGGAGAAC 
TCGGATAACG CATTTTATGA GGACAATTTT GCTGTTGCAA ATAGTACAAG TGAAGCTGAA 
TGTGATACTC AGGTGATTAA GAAGGAAAAA GAAAGCAGAG GTATACCTGT AATGAAAAAC 
AATCCGCACA CGAGGATGGT AGCTGTTCAG GTGAATAGTG AAGTAACTGA TAGGGAAACA 
AATACTTTGC CTTTTCATCC ATTTGAGAAG CAACAAGGAG ATATTCTACG TATGGAAAAA 
GAGCATCAAG TATTAAAAGA GCAACTTAAA GAAGCACAGG AAAAATACGA ACAATTACAG 
AACCGGAGCT CAGAGGAAAT CAGTGTTTTA GAAGAGCTGA TAAAGAAGAT TGTTGAAGAG 
AAAGGGATAT TTAAGAAAGA AGTGGAGTGG CTTCATCAAT CTTTAGAAAT AAAAGTAAAA 
AAATGGCAAC AGGAGAAAAA GGAAAATCAA GAGAACTTAA AAGCAATGAG GAACACAGCC 
AAAAAGCATA TGGATACCAA TGACAGGTAT TCAAAGACCA TTGATGAGAA GGAGAAGCAG 
TATAACACAT GTTTGAATAC TTATCTTGAG ACCAGTAATA AATTTGCTAA TGAAAAAGTA 
AAATTGGAAG AACTTATTAA AAAGAGTCAA GATGACCTCA AGGAGCATGA GAAAAGAGCT 
GTTACAGCAG AGATATCAGT ACTCAAAAAC TGGAAAGAAA GCGAACTGTG CAAACTAAGT 
GGCATAGTTG CCAGAGCAGA AACAAATCTC AAGATGTTGA AGTCACTAAG CAGCAACTCA 
GCTTCAGCGC TTCCTGACTT AAAGTCACAG ATTAATTATT GGGAAGTATT TATTTCAAAT 
ATAAAGAAAC AAATGGAGAA AGTAAAGGTT GGGTACGAAG AAAAAATTGA GAAGGTGAAA 
AATGGTGTTC GAAACTGCCT CACCAAAATA GAAGCTGCGG ATCTTTCATC TCTTCCCAGT 
GTCTTAAGAA AGCAAAGCAG ACCTGCTGTC AGTGATCCAG CCCTTGTCCT GTGTTCCTCT 
GCATCTGCAC AGCCTAATTT ACTTCCTGCA GTTTCGAGTT CTGAAGATCA AGGTCCAACA 
ACTGCTTCAT TTAATAGCCA GGCTGGAGTT AAGCCTGCAA ATGCAAATTC TAAGGTTTGC 
TCTGCAAGTG ATGGATCAGT GAAAACAAAC TCCAAAGCTC GGACAGGATC GTATGCTGAT 
AAAGTTTCGC TAAATTCTAT GCCAAAGGTT TCAGGAAGCA ATACTCAGAA TATACAGCCA 
AACCAAACCC ACCAAGATGA TTCAGCTACA CAGATGAAGC CCACTTCAAA CAACACATTT 
GAAAATATTA TTGCTCATCT ACAAACTATA TTTCCGGGCT ACAGCAGTTC AGAGTTCACG 
AGGTTCATAA AAGATGTACA AAGAAGAAGT GGGAATACAT TAAGTTTGAG CTCCACTGAA 
ATTCTAAGCA GAGTGACAGA TCTCATTCTG GACCAGCAAA ATGAGGCATC AGCTTCTGTA 
GGGAGACCTA TGATGTCTGC ATCATCCGCT TCAGCGGGAC AGACGGGAAC TCAAAGTCAG 
GAAGCAGCTG CAGAAGGAAA TATTTCCAGC GCACCCAAGG CAAGGCCTGC ACACAAAAGC 
ACCTCCAGTA AAAACACGCT GCCATCTCAG CCAAATCTCC AGCCATGGGG GAATGCTGGA 
GCAACACCGA AATCTAAATG GAAAAAACTA GACTATATAC CTTCCAGCGA TGATCCGTGC 
ACCATCTGCC ATGATGAGTT AAGCAGAGAC TCATGTGAAC TGGAGTGTGG GCATCATTTT 
CACAGAGAAT GCATTAGAAA ATGGCTGAAG GAACATTCGA GTACCTGCCC TATCTGTCGC 
ATCCATGTTC TGTTACCTGA AGACTTTCCT GAGCTCCCTG CACGCAACAA ATATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_416727.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_416727.4 Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

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CloneID	OGa02730
Clone ID Related Accession (Same CDS sequence)	XM_416727.5 , XM_416727.6
Accession Version	XM_416727.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416727.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGCA GTTTACAGAT TTTAGACTGG 
AATTGGAAGA AACTCTGTGA TTTAGAGCTT GTGGAAGACC TTGTTGATTT GACTAAAAAA 
GTGGTTAATG TTCCACATTT TATTGGGGGC ATACTGAGAA TTGGCAGCAG GTTAGAAAAC 
GGTTTCTTCG ATGTTAGGGA TGCACTTGAT TGGATCAGAT GTACAGGTGA TATCAATATT 
CTGCAGAAGC TGGAGAAACT TGGTGATTTT GGTTGGCTCT ATCTTGAAAC TTTCTTTGGT 
GAATGCAAAC ATTATATTAC TAAAGTTGCT TTGGAAGATT GTAATCTAGT TGAAGAATTT 
AAAGCTGTCA CCTGTGAAAA CTGCAGGAAG AAGAGTGAAT CTATGAAACA GAAAGGAAAT 
GAGGAGTTCT CCCAAGGAAA ATATGATCAT GCTATAATAT CATATACTAA AGCCATAGAG 
TTTTGTCCTG CAAACCATCT TCTGTATGGA AACCGAGCTC TTTGTTTAAT TCTCACGAAG 
CAGTACAAGA GAGCCCTTGC TGATGGAAAA AGGGCCACTA TTTTGAAGCC TAATTGGCCA 
AAGGGTCATT ACCACTTCTG TAAGGCGCTG TCATTACTGG GGGAGCATGA ACTGGCTTTA 
GAAGCTAATG AGAGAGCTCA GGAACTGTGC AAGAATATTC TTGATGGATT TAAGGATCTT 
ATTCAACAAA ATGACAAGTT AAAGAAGACA TTAGAGGAAA TCAAGGGTAC TAAAGAATAT 
AAGCAGAAAC CAAAGAATTT GCTTCTTGAG AAAAAGGACT GTGGTTCTTC AAAATCGCAT 
TTGATCGTTT CTCAAGAACA AAAAGAAATA GAGAAGGACA GAAAACAAAT GCAGTTTGAT 
CATAAAGATG ACCATTATCA TCAAAAACAC GATACCACCA TAAGATATCC TACAGATGGA 
ATTCCAAGAC TATCAGTGAC CAACATGCCC AGGACAGAAA ACAAAGTGTG GTCTCTGGAC 
TTTCAACCAA GCCAAAGTCA CCAACACAAA GGAAGGTCGA AGAACAAGCC TGGTGAACCT 
GAAAAGAAAC AGGATCACCT TGATTTGAAG ATAGACTCTA AAAGCATCCA AGATAAACTC 
GTCTGTACTG TGCACCAGGT GGATTCAACT ATGTTGCTTG AAGTGGTTAA ATCCCTCGTT 
CATGATGGTT ACACAGCCTT GATGGACCAG AGGTGTCACA GTGCTGAACA AATGTTCTCA 
CAACTGTTGA ACATTCTCAA TCCTTCGGAA TTAAAGCAAT TGAATCTTCG TGTTATTAAT 
TATGTTGTAA TCATCTATGG ACATGCCACT TCTCTGCTTG GAATAGGACA GCCCGAGGCG 
TTGACAAAGG CTGAAGACCA GTTTAAAAGA ATAATTATAC AGTACCAGCA AGAAAGATTT 
GATTGTCTGG CATACTATGG AATTGGAAAA GTATACCTCA GACAAAACAG ATTTTTAGAT 
GCGCTCAATC AGTTTAAGAA GTCACAAACA ATGATTAATC ATAAGATTGT ACCTGGAGTA 
TTAACTTGGC CCACGACATC TCAGGTCATT GAAGAAACAC GAACAGAGAA TTTACAGTTG 
TTCTTGAAGA ACTGCATTGA GGAATGCAAG TTCCCTCCAG AACCGGATGC AATTTGTCGC 
TATCAGCAGT GCCATGGCCA CTCCAAAATC CAAATATTTT TTACAGATCC AGACTTTAAG 
GGTTTTATAC GTATTACTTG CTGTCAGCAA TGTAGAGTCG AGTTTCATAC CAGCTGTTGG 
AAAAAGTTGA AAACTGCAAA CTACAGTGAT AAGAATGACA AGGATTTTCT TCAAGAATTA 
TGTTTCACTC CCGACTGTAG AGGCCTTATT TCAAAAATAG TTATTTTCAG TTCTTCTGGT 
TTGGTAAAAC GTGAATTTGA ACAAAAAATC ACAAAACCCA AGGAACCACC AAGAGCCATT 
ATTAAACAGA AATGTTCAAG TTTTAGGAAG TTAAAAAGGA AAGAAGAAAA AAAAATACGA 
AGAAAACGTG CACGGGAAGA TGCACTGAAT TCTTCTAAAC AGAAAATAGA AGAAAATCAG 
ATGCGAAATG AACTGTCTCA GTACCGTAAT CGCAGTGATT ATGCAGGTTA TACTGGTGAT 
CAAGTCCTGC AGCGTATCAT TCAAAATGCT GAGCAAATAA AGACTGCTGT TCGTGATGCC 
TCCAAACTGT TAAAGGAATT ACTTTCCTGG TTTGTAATCA GTGAAGAGGA TTACACATCA 
TATTGCAAAA CTAGCAGCAT GTCACATGCT GTGGTGGAGC AGCTCATCGG TTACTTGATT 
CAGGAAGAAA ACAGAGTGAA AACAAGGAGT TTTATCCGTG TCCTGAGTGA AATGGAAGAA 
GTGGATCCCA AATTACAGAG GTGGATGAAA CACCTTAATG ATTTGGGGTT GAGAGCTACA 
GTGAACTTTA TCCATCAGTA TGGACAATTT TTAAAACAAC TTGACTTTTC TATTTTATCT 
GCACCCTGGA ATGAGAAGTA TGGTAGTAAA TTTGACTACG TGTCAAGTAG TTCTGAAGGC 
AAAGAAATTC TTGACTATTT TTTAAAACCA CCTCTAGAAA AGGTCCGTTG TTTTATATGG 
CTGTTAGAAG ACAACAGAGA ACATTTTCCA TCTCTTCATC AAGCTTTAGA TGAATTCTTT 
GATAAAACGG ACAACCCTAC CATCATATTA AAGAAGCATG GGAATGAAGA TATATTGACT 
AATGGTATCA AAGTTAAAAA CAAAAGCAGA AAGAAGAACT ATAAAGACTC ACGGTCTGTT 
ATAGTGTTAT CCAGTGGAGT TAGTACAGCA CCACAAGAAG AAAATACATT TATAGAAGAA 
ACTACATTAG ATTGTACGTA TTATGAACCA TGTATTATCC CAGAATATCT TCGGAAGCAA 
CTAGAGGAGT TTGAATCCGT GTATGACATT TCAAACAGTA ACAGCTATCA AAGACTGGAT 
GATAATCTAA ATCAAACCTG TGAAAGTTTA TATGACTATT TCTCTCAGAT ATTGGAAGAG 
CATGGACCGA TGGAATTAAA TAATAAGTTG CTGGTTGGGG AATATGAACA TTTCCCTGAA 
GAAGCTCGAA AGATTGTAGA AGATGAGGGT GGTTTGAAAT CTTTTCTTCT GAAATCCCAT 
CGTTTCGTCA TGGTGGATAA TTTTATTAGC TTAAGACATG ATGTTCTTAT TAAAGAAAAT 
AAAAACAGAG ACGAGACTGA AAATAATGAA GAAGGAAATT ATATTTTCTG TGATGCGCAA 
GAAAATTCTT TCCAGAACAA GATACAGTTA AATCCAGCTG CTAAGGAATT CAAACCACTG 
TCTTATCCTG AACAACCTCA TCTATCAACA TCTACTGACA TATCATTAGA AAGCTGTGAG 
ACTCCACAAT GTTTGCCCTA CTCTTTGCCT ACTTCATGGC AATCAATGCT TAGTCAGGAT 
ATTGATCCTG TCGAAAATAC GCCTTATTTT TCAAATGTTT TATTGTATCA AGATTTTCAG 
AATCAAAGCA GTTTTTTGCC TCAGACTTCC TGGGGTTATC AGTGTGAAAG AACATCGCCA 
GTGCCTTCTC CAGTGCCTCT CCTGTCAAAT GTTGCCAAGC AACCTGGTTA TATTTATGCT 
GATTGTGTCG CTCATCAGAA TAATGATGAA TTAGCAATTT CAGACAGAAA TTATGTCAGC 
AACAGCTTTA TACCAGCTGA AATAGAAACA TACAGAGACC CTCAGATCCT GGTGGAGAAC 
TCGGATAACG CATTTTATGA GGACAATTTT GCTGTTGCAA ATAGTACAAG TGAAGCTGAA 
TGTGATACTC AGGTGATTAA GAAGGAAAAA GAAAGCAGAG GTATACCTGT AATGAAAAAC 
AATCCGCACA CGAGGATGGT AGCTGTTCAG GTGAATAGTG AAGTAACTGA TAGGGAAACA 
AATACTTTGC CTTTTCATCC ATTTGAGAAG CAACAAGGAG ATATTCTACG TATGGAAAAA 
GAGCATCAAG TATTAAAAGA GCAACTTAAA GAAGCACAGG AAAAATACGA ACAATTACAG 
AACCGGAGCT CAGAGGAAAT CAGTGTTTTA GAAGAGCTGA TAAAGAAGAT TGTTGAAGAG 
AAAGGGATAT TTAAGAAAGA AGTGGAGTGG CTTCATCAAT CTTTAGAAAT AAAAGTAAAA 
AAATGGCAAC AGGAGAAAAA GGAAAATCAA GAGAACTTAA AAGCAATGAG GAACACAGCC 
AAAAAGCATA TGGATACCAA TGACAGGTAT TCAAAGACCA TTGATGAGAA GGAGAAGCAG 
TATAACACAT GTTTGAATAC TTATCTTGAG ACCAGTAATA AATTTGCTAA TGAAAAAGTA 
AAATTGGAAG AACTTATTAA AAAGAGTCAA GATGACCTCA AGGAGCATGA GAAAAGAGCT 
GTTACAGCAG AGATATCAGT ACTCAAAAAC TGGAAAGAAA GCGAACTGTG CAAACTAAGT 
GGCATAGTTG CCAGAGCAGA AACAAATCTC AAGATGTTGA AGTCACTAAG CAGCAACTCA 
GCTTCAGCGC TTCCTGACTT AAAGTCACAG ATTAATTATT GGGAAGTATT TATTTCAAAT 
ATAAAGAAAC AAATGGAGAA AGTAAAGGTT GGGTACGAAG AAAAAATTGA GAAGGTGAAA 
AATGGTGTTC GAAACTGCCT CACCAAAATA GAAGCTGCGG ATCTTTCATC TCTTCCCAGT 
GTCTTAAGAA AGCAAAGCAG ACCTGCTGTC AGTGATCCAG CCCTTGTCCT GTGTTCCTCT 
GCATCTGCAC AGCCTAATTT ACTTCCTGCA GTTTCGAGTT CTGAAGATCA AGGTCCAACA 
ACTGCTTCAT TTAATAGCCA GGCTGGAGTT AAGCCTGCAA ATGCAAATTC TAAGGTTTGC 
TCTGCAAGTG ATGGATCAGT GAAAACAAAC TCCAAAGCTC GGACAGGATC GTATGCTGAT 
AAAGTTTCGC TAAATTCTAT GCCAAAGGTT TCAGGAAGCA ATACTCAGAA TATACAGCCA 
AACCAAACCC ACCAAGATGA TTCAGCTACA CAGATGAAGC CCACTTCAAA CAACACATTT 
GAAAATATTA TTGCTCATCT ACAAACTATA TTTCCGGGCT ACAGCAGTTC AGAGTTCACG 
AGGTTCATAA AAGATGTACA AAGAAGAAGT GGGAATACAT TAAGTTTGAG CTCCACTGAA 
ATTCTAAGCA GAGTGACAGA TCTCATTCTG GACCAGCAAA ATGAGGCATC AGCTTCTGTA 
GGGAGACCTA TGATGTCTGC ATCATCCGCT TCAGCGGGAC AGACGGGAAC TCAAAGTCAG 
GAAGCAGCTG CAGAAGGAAA TATTTCCAGC GCACCCAAGG CAAGGCCTGC ACACAAAAGC 
ACCTCCAGTA AAAACACGCT GCCATCTCAG CCAAATCTCC AGCCATGGGG GAATGCTGGA 
GCAACACCGA AATCTAAATG GAAAAAACTA GACTATATAC CTTCCAGCGA TGATCCGTGC 
ACCATCTGCC ATGATGAGTT AAGCAGAGAC TCATGTGAAC TGGAGTGTGG GCATCATTTT 
CACAGAGAAT GCATTAGAAA ATGGCTGAAG GAACATTCGA GTACCTGCCC TATCTGTCGC 
ATCCATGTTC TGTTACCTGA AGACTTTCCT GAGCTCCCTG CACGCAACAA ATATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_416727.4
CDS	88..6027
Translation	MVGAGARTMGEPLSESEFAALAFSAGYKSCCELGHGVFVQRSPHKPKTDVDPCEVWCRQPVDKLREYCNVIKIRIFWPLLFQSESNYLVADWGSLQILDWNWKKLCDLELVEDLVDLTKKVVNVPHFIGGILRIGSRLENGFFDVRDALDWIRCTGDINILQKLEKLGDFGWLYLETFFGECKHYITKVALEDCNLVEEFKAVTCENCRKKSESMKQKGNEEFSQGKYDHAIISYTKAIEFCPANHLLYGNRALCLILTKQYKRALADGKRATILKPNWPKGHYHFCKALSLLGEHELALEANERAQELCKNILDGFKDLIQQNDKLKKTLEEIKGTKEYKQKPKNLLLEKKDCGSSKSHLIVSQEQKEIEKDRKQMQFDHKDDHYHQKHDTTIRYPTDGIPRLSVTNMPRTENKVWSLDFQPSQSHQHKGRSKNKPGEPEKKQDHLDLKIDSKSIQDKLVCTVHQVDSTMLLEVVKSLVHDGYTALMDQRCHSAEQMFSQLLNILNPSELKQLNLRVINYVVIIYGHATSLLGIGQPEALTKAEDQFKRIIIQYQQERFDCLAYYGIGKVYLRQNRFLDALNQFKKSQTMINHKIVPGVLTWPTTSQVIEETRTENLQLFLKNCIEECKFPPEPDAICRYQQCHGHSKIQIFFTDPDFKGFIRITCCQQCRVEFHTSCWKKLKTANYSDKNDKDFLQELCFTPDCRGLISKIVIFSSSGLVKREFEQKITKPKEPPRAIIKQKCSSFRKLKRKEEKKIRRKRAREDALNSSKQKIEENQMRNELSQYRNRSDYAGYTGDQVLQRIIQNAEQIKTAVRDASKLLKELLSWFVISEEDYTSYCKTSSMSHAVVEQLIGYLIQEENRVKTRSFIRVLSEMEEVDPKLQRWMKHLNDLGLRATVNFIHQYGQFLKQLDFSILSAPWNEKYGSKFDYVSSSSEGKEILDYFLKPPLEKVRCFIWLLEDNREHFPSLHQALDEFFDKTDNPTIILKKHGNEDILTNGIKVKNKSRKKNYKDSRSVIVLSSGVSTAPQEENTFIEETTLDCTYYEPCIIPEYLRKQLEEFESVYDISNSNSYQRLDDNLNQTCESLYDYFSQILEEHGPMELNNKLLVGEYEHFPEEARKIVEDEGGLKSFLLKSHRFVMVDNFISLRHDVLIKENKNRDETENNEEGNYIFCDAQENSFQNKIQLNPAAKEFKPLSYPEQPHLSTSTDISLESCETPQCLPYSLPTSWQSMLSQDIDPVENTPYFSNVLLYQDFQNQSSFLPQTSWGYQCERTSPVPSPVPLLSNVAKQPGYIYADCVAHQNNDELAISDRNYVSNSFIPAEIETYRDPQILVENSDNAFYEDNFAVANSTSEAECDTQVIKKEKESRGIPVMKNNPHTRMVAVQVNSEVTDRETNTLPFHPFEKQQGDILRMEKEHQVLKEQLKEAQEKYEQLQNRSSEEISVLEELIKKIVEEKGIFKKEVEWLHQSLEIKVKKWQQEKKENQENLKAMRNTAKKHMDTNDRYSKTIDEKEKQYNTCLNTYLETSNKFANEKVKLEELIKKSQDDLKEHEKRAVTAEISVLKNWKESELCKLSGIVARAETNLKMLKSLSSNSASALPDLKSQINYWEVFISNIKKQMEKVKVGYEEKIEKVKNGVRNCLTKIEAADLSSLPSVLRKQSRPAVSDPALVLCSSASAQPNLLPAVSSSEDQGPTTASFNSQAGVKPANANSKVCSASDGSVKTNSKARTGSYADKVSLNSMPKVSGSNTQNIQPNQTHQDDSATQMKPTSNNTFENIIAHLQTIFPGYSSSEFTRFIKDVQRRSGNTLSLSSTEILSRVTDLILDQQNEASASVGRPMMSASSASAGQTGTQSQEAAAEGNISSAPKARPAHKSTSSKNTLPSQPNLQPWGNAGATPKSKWKKLDYIPSSDDPCTICHDELSRDSCELECGHHFHRECIRKWLKEHSSTCPICRIHVLLPEDFPELPARNKYA

Target ORF information:

RefSeq Version	XM_416727.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_416727.6

ORF Insert Sequence:

ATGGTAGGGG CTGGAGCTCG AACCATGGGT GAACCTTTGT CTGAATCTGA GTTTGCGGCC 
TTGGCTTTCA GTGCTGGTTA TAAATCCTGC TGTGAATTGG GTCATGGAGT ATTTGTACAA 
CGTTCTCCAC ATAAACCTAA AACTGATGTT GACCCCTGTG AAGTGTGGTG CCGTCAGCCG 
GTTGACAAAC TAAGAGAATA TTGCAACGTT ATTAAAATAC GCATCTTTTG GCCTCTTCTC 
TTCCAAAGTG AAAGTAATTA TTTGGTAGCT GACTGGGGCA GTTTACAGAT TTTAGACTGG 
AATTGGAAGA AACTCTGTGA TTTAGAGCTT GTGGAAGACC TTGTTGATTT GACTAAAAAA 
GTGGTTAATG TTCCACATTT TATTGGGGGC ATACTGAGAA TTGGCAGCAG GTTAGAAAAC 
GGTTTCTTCG ATGTTAGGGA TGCACTTGAT TGGATCAGAT GTACAGGTGA TATCAATATT 
CTGCAGAAGC TGGAGAAACT TGGTGATTTT GGTTGGCTCT ATCTTGAAAC TTTCTTTGGT 
GAATGCAAAC ATTATATTAC TAAAGTTGCT TTGGAAGATT GTAATCTAGT TGAAGAATTT 
AAAGCTGTCA CCTGTGAAAA CTGCAGGAAG AAGAGTGAAT CTATGAAACA GAAAGGAAAT 
GAGGAGTTCT CCCAAGGAAA ATATGATCAT GCTATAATAT CATATACTAA AGCCATAGAG 
TTTTGTCCTG CAAACCATCT TCTGTATGGA AACCGAGCTC TTTGTTTAAT TCTCACGAAG 
CAGTACAAGA GAGCCCTTGC TGATGGAAAA AGGGCCACTA TTTTGAAGCC TAATTGGCCA 
AAGGGTCATT ACCACTTCTG TAAGGCGCTG TCATTACTGG GGGAGCATGA ACTGGCTTTA 
GAAGCTAATG AGAGAGCTCA GGAACTGTGC AAGAATATTC TTGATGGATT TAAGGATCTT 
ATTCAACAAA ATGACAAGTT AAAGAAGACA TTAGAGGAAA TCAAGGGTAC TAAAGAATAT 
AAGCAGAAAC CAAAGAATTT GCTTCTTGAG AAAAAGGACT GTGGTTCTTC AAAATCGCAT 
TTGATCGTTT CTCAAGAACA AAAAGAAATA GAGAAGGACA GAAAACAAAT GCAGTTTGAT 
CATAAAGATG ACCATTATCA TCAAAAACAC GATACCACCA TAAGATATCC TACAGATGGA 
ATTCCAAGAC TATCAGTGAC CAACATGCCC AGGACAGAAA ACAAAGTGTG GTCTCTGGAC 
TTTCAACCAA GCCAAAGTCA CCAACACAAA GGAAGGTCGA AGAACAAGCC TGGTGAACCT 
GAAAAGAAAC AGGATCACCT TGATTTGAAG ATAGACTCTA AAAGCATCCA AGATAAACTC 
GTCTGTACTG TGCACCAGGT GGATTCAACT ATGTTGCTTG AAGTGGTTAA ATCCCTCGTT 
CATGATGGTT ACACAGCCTT GATGGACCAG AGGTGTCACA GTGCTGAACA AATGTTCTCA 
CAACTGTTGA ACATTCTCAA TCCTTCGGAA TTAAAGCAAT TGAATCTTCG TGTTATTAAT 
TATGTTGTAA TCATCTATGG ACATGCCACT TCTCTGCTTG GAATAGGACA GCCCGAGGCG 
TTGACAAAGG CTGAAGACCA GTTTAAAAGA ATAATTATAC AGTACCAGCA AGAAAGATTT 
GATTGTCTGG CATACTATGG AATTGGAAAA GTATACCTCA GACAAAACAG ATTTTTAGAT 
GCGCTCAATC AGTTTAAGAA GTCACAAACA ATGATTAATC ATAAGATTGT ACCTGGAGTA 
TTAACTTGGC CCACGACATC TCAGGTCATT GAAGAAACAC GAACAGAGAA TTTACAGTTG 
TTCTTGAAGA ACTGCATTGA GGAATGCAAG TTCCCTCCAG AACCGGATGC AATTTGTCGC 
TATCAGCAGT GCCATGGCCA CTCCAAAATC CAAATATTTT TTACAGATCC AGACTTTAAG 
GGTTTTATAC GTATTACTTG CTGTCAGCAA TGTAGAGTCG AGTTTCATAC CAGCTGTTGG 
AAAAAGTTGA AAACTGCAAA CTACAGTGAT AAGAATGACA AGGATTTTCT TCAAGAATTA 
TGTTTCACTC CCGACTGTAG AGGCCTTATT TCAAAAATAG TTATTTTCAG TTCTTCTGGT 
TTGGTAAAAC GTGAATTTGA ACAAAAAATC ACAAAACCCA AGGAACCACC AAGAGCCATT 
ATTAAACAGA AATGTTCAAG TTTTAGGAAG TTAAAAAGGA AAGAAGAAAA AAAAATACGA 
AGAAAACGTG CACGGGAAGA TGCACTGAAT TCTTCTAAAC AGAAAATAGA AGAAAATCAG 
ATGCGAAATG AACTGTCTCA GTACCGTAAT CGCAGTGATT ATGCAGGTTA TACTGGTGAT 
CAAGTCCTGC AGCGTATCAT TCAAAATGCT GAGCAAATAA AGACTGCTGT TCGTGATGCC 
TCCAAACTGT TAAAGGAATT ACTTTCCTGG TTTGTAATCA GTGAAGAGGA TTACACATCA 
TATTGCAAAA CTAGCAGCAT GTCACATGCT GTGGTGGAGC AGCTCATCGG TTACTTGATT 
CAGGAAGAAA ACAGAGTGAA AACAAGGAGT TTTATCCGTG TCCTGAGTGA AATGGAAGAA 
GTGGATCCCA AATTACAGAG GTGGATGAAA CACCTTAATG ATTTGGGGTT GAGAGCTACA 
GTGAACTTTA TCCATCAGTA TGGACAATTT TTAAAACAAC TTGACTTTTC TATTTTATCT 
GCACCCTGGA ATGAGAAGTA TGGTAGTAAA TTTGACTACG TGTCAAGTAG TTCTGAAGGC 
AAAGAAATTC TTGACTATTT TTTAAAACCA CCTCTAGAAA AGGTCCGTTG TTTTATATGG 
CTGTTAGAAG ACAACAGAGA ACATTTTCCA TCTCTTCATC AAGCTTTAGA TGAATTCTTT 
GATAAAACGG ACAACCCTAC CATCATATTA AAGAAGCATG GGAATGAAGA TATATTGACT 
AATGGTATCA AAGTTAAAAA CAAAAGCAGA AAGAAGAACT ATAAAGACTC ACGGTCTGTT 
ATAGTGTTAT CCAGTGGAGT TAGTACAGCA CCACAAGAAG AAAATACATT TATAGAAGAA 
ACTACATTAG ATTGTACGTA TTATGAACCA TGTATTATCC CAGAATATCT TCGGAAGCAA 
CTAGAGGAGT TTGAATCCGT GTATGACATT TCAAACAGTA ACAGCTATCA AAGACTGGAT 
GATAATCTAA ATCAAACCTG TGAAAGTTTA TATGACTATT TCTCTCAGAT ATTGGAAGAG 
CATGGACCGA TGGAATTAAA TAATAAGTTG CTGGTTGGGG AATATGAACA TTTCCCTGAA 
GAAGCTCGAA AGATTGTAGA AGATGAGGGT GGTTTGAAAT CTTTTCTTCT GAAATCCCAT 
CGTTTCGTCA TGGTGGATAA TTTTATTAGC TTAAGACATG ATGTTCTTAT TAAAGAAAAT 
AAAAACAGAG ACGAGACTGA AAATAATGAA GAAGGAAATT ATATTTTCTG TGATGCGCAA 
GAAAATTCTT TCCAGAACAA GATACAGTTA AATCCAGCTG CTAAGGAATT CAAACCACTG 
TCTTATCCTG AACAACCTCA TCTATCAACA TCTACTGACA TATCATTAGA AAGCTGTGAG 
ACTCCACAAT GTTTGCCCTA CTCTTTGCCT ACTTCATGGC AATCAATGCT TAGTCAGGAT 
ATTGATCCTG TCGAAAATAC GCCTTATTTT TCAAATGTTT TATTGTATCA AGATTTTCAG 
AATCAAAGCA GTTTTTTGCC TCAGACTTCC TGGGGTTATC AGTGTGAAAG AACATCGCCA 
GTGCCTTCTC CAGTGCCTCT CCTGTCAAAT GTTGCCAAGC AACCTGGTTA TATTTATGCT 
GATTGTGTCG CTCATCAGAA TAATGATGAA TTAGCAATTT CAGACAGAAA TTATGTCAGC 
AACAGCTTTA TACCAGCTGA AATAGAAACA TACAGAGACC CTCAGATCCT GGTGGAGAAC 
TCGGATAACG CATTTTATGA GGACAATTTT GCTGTTGCAA ATAGTACAAG TGAAGCTGAA 
TGTGATACTC AGGTGATTAA GAAGGAAAAA GAAAGCAGAG GTATACCTGT AATGAAAAAC 
AATCCGCACA CGAGGATGGT AGCTGTTCAG GTGAATAGTG AAGTAACTGA TAGGGAAACA 
AATACTTTGC CTTTTCATCC ATTTGAGAAG CAACAAGGAG ATATTCTACG TATGGAAAAA 
GAGCATCAAG TATTAAAAGA GCAACTTAAA GAAGCACAGG AAAAATACGA ACAATTACAG 
AACCGGAGCT CAGAGGAAAT CAGTGTTTTA GAAGAGCTGA TAAAGAAGAT TGTTGAAGAG 
AAAGGGATAT TTAAGAAAGA AGTGGAGTGG CTTCATCAAT CTTTAGAAAT AAAAGTAAAA 
AAATGGCAAC AGGAGAAAAA GGAAAATCAA GAGAACTTAA AAGCAATGAG GAACACAGCC 
AAAAAGCATA TGGATACCAA TGACAGGTAT TCAAAGACCA TTGATGAGAA GGAGAAGCAG 
TATAACACAT GTTTGAATAC TTATCTTGAG ACCAGTAATA AATTTGCTAA TGAAAAAGTA 
AAATTGGAAG AACTTATTAA AAAGAGTCAA GATGACCTCA AGGAGCATGA GAAAAGAGCT 
GTTACAGCAG AGATATCAGT ACTCAAAAAC TGGAAAGAAA GCGAACTGTG CAAACTAAGT 
GGCATAGTTG CCAGAGCAGA AACAAATCTC AAGATGTTGA AGTCACTAAG CAGCAACTCA 
GCTTCAGCGC TTCCTGACTT AAAGTCACAG ATTAATTATT GGGAAGTATT TATTTCAAAT 
ATAAAGAAAC AAATGGAGAA AGTAAAGGTT GGGTACGAAG AAAAAATTGA GAAGGTGAAA 
AATGGTGTTC GAAACTGCCT CACCAAAATA GAAGCTGCGG ATCTTTCATC TCTTCCCAGT 
GTCTTAAGAA AGCAAAGCAG ACCTGCTGTC AGTGATCCAG CCCTTGTCCT GTGTTCCTCT 
GCATCTGCAC AGCCTAATTT ACTTCCTGCA GTTTCGAGTT CTGAAGATCA AGGTCCAACA 
ACTGCTTCAT TTAATAGCCA GGCTGGAGTT AAGCCTGCAA ATGCAAATTC TAAGGTTTGC 
TCTGCAAGTG ATGGATCAGT GAAAACAAAC TCCAAAGCTC GGACAGGATC GTATGCTGAT 
AAAGTTTCGC TAAATTCTAT GCCAAAGGTT TCAGGAAGCA ATACTCAGAA TATACAGCCA 
AACCAAACCC ACCAAGATGA TTCAGCTACA CAGATGAAGC CCACTTCAAA CAACACATTT 
GAAAATATTA TTGCTCATCT ACAAACTATA TTTCCGGGCT ACAGCAGTTC AGAGTTCACG 
AGGTTCATAA AAGATGTACA AAGAAGAAGT GGGAATACAT TAAGTTTGAG CTCCACTGAA 
ATTCTAAGCA GAGTGACAGA TCTCATTCTG GACCAGCAAA ATGAGGCATC AGCTTCTGTA 
GGGAGACCTA TGATGTCTGC ATCATCCGCT TCAGCGGGAC AGACGGGAAC TCAAAGTCAG 
GAAGCAGCTG CAGAAGGAAA TATTTCCAGC GCACCCAAGG CAAGGCCTGC ACACAAAAGC 
ACCTCCAGTA AAAACACGCT GCCATCTCAG CCAAATCTCC AGCCATGGGG GAATGCTGGA 
GCAACACCGA AATCTAAATG GAAAAAACTA GACTATATAC CTTCCAGCGA TGATCCGTGC 
ACCATCTGCC ATGATGAGTT AAGCAGAGAC TCATGTGAAC TGGAGTGTGG GCATCATTTT 
CACAGAGAAT GCATTAGAAA ATGGCTGAAG GAACATTCGA GTACCTGCCC TATCTGTCGC 
ATCCATGTTC TGTTACCTGA AGACTTTCCT GAGCTCCCTG CACGCAACAA ATATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TTC3
Entrez Gene ID	418518
Full Name	tetratricopeptide repeat domain 3
General protein information	Preferred Names tetratricopeptide repeat domain 3 Names E3 ubiquitin-protein ligase TTC3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Rattus norvegicus (Norway rat)	Ttc3	NP_001101785.1
Bos taurus (cattle)	TTC3	NP_001103237.1
Xenopus tropicalis (tropical clawed frog)	ttc3	XP_002941578.2
	TTC3	XP_001098049.2
Gallus gallus (chicken)	TTC3	XP_416727.4
Danio rerio (zebrafish)	LOC100329450	XP_005172636.1
Pan troglodytes (chimpanzee)	TTC3	XP_001169932.1
Canis lupus familiaris (dog)	TTC3	XP_849567.2
Mus musculus (house mouse)	Ttc3	NP_033467.2
Homo sapiens (human)	TTC3	NP_001001894.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TTC3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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