TTC3 cDNA ORF clone,

Gene
Clones
gRNAs

The following TTC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15645	XM_005172576.3 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49197	XM_009305046.2 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49197	XM_009305045.2 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49197	XM_009305044.2 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49197	XM_009305043.2 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa49198	XM_009305047.2 Latest version!	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTC3 ( XM_005172576.3 ) cDNA ORF clone, -> XP_005172633.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X5, mRNA.

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CloneID	ODa15645
Clone ID Related Accession (Same CDS sequence)	XM_005172576.3
Accession Version	XM_005172576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGATG 
AACATCACTG CTGGAGACGC TGCAGATCTG AGCTGTCCTC CGTCAGACTG CACACTCAGA 
GCCGCTGGAA CTCCACCGCT CACACACGTC TGGAAGAACG TCCCGGAGAA ACACCGCAAC 
TCTGCACTGG TGCTCAACAT GGAGGACCCG TGCATCATCT GCCATGAGGA CATGAGTGCG 
GAGGATCTGT GTGTGCTGGA GTGCCGCCAC AGCTTCCACA GAGAGTGTAT AAAGTCGTGG 
CTGAAGGAGC AGAGCACCTG TCCCACCTGT CGAGAGCATG CGCTGCTGCC GGAGGACTTT 
CCCCTGCTGC CTGGCAGACA ACGCAGAACA CACACCGCCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172633.1
CDS	115..5199
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGMRAGHSRDEDDEDELFSEEDSLMFLNDMDPFSVPDHLRYQLAEFEENYSGSAQRSYYERMLDNHPDPTKETLYDYFAQVLEEHGPLCATDPLLVGELQNFPLEAQQQIADAGGLQAFLLQSLRFTMTERLIGLMKHAVCLSESSNTHSQILNPSASEFWPLSGEPPSSTGEPALLLPDPYTDEEDQLSAAEMTESDTEETRAEKHTAVRAQDFQDRTDVSVNTEPCAPFENNSGELMGLQKRRLYLLEELQRIRADGEAEQQRRQEELTELQEDTHNISQRMQIASTELSMFQQKLEEEVKKDQQEKKENQETLRSLKMEIKELVELQESLSRDISVRNRQYEAELELFLDVSNQCAAERLSLEDEIKRFRDACVKAERRSASAQLCVLQSRREHALRRLRMTVSEGKMVVKHLSDVALSSPSAALLSAIEDWKRHVSEAEGRIHATEEECEAQMQAVQQGSRLCSLPDISTPSVPSAPALPALQTPVSSAAPEESSSRRSAAPQHLSVYERIVERLHIMFPHYDRLVLNKFLQEVRSSCSGGVLSSLTYNEVINRVAQLILDHQENICQMNITAGDAADLSCPPSDCTLRAAGTPPLTHVWKNVPEKHRNSALVLNMEDPCIICHEDMSAEDLCVLECRHSFHRECIKSWLKEQSTCPTCREHALLPEDFPLLPGRQRRTHTAS

Target ORF information:

RefSeq Version	XM_005172576.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172576.3

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGATG 
AACATCACTG CTGGAGACGC TGCAGATCTG AGCTGTCCTC CGTCAGACTG CACACTCAGA 
GCCGCTGGAA CTCCACCGCT CACACACGTC TGGAAGAACG TCCCGGAGAA ACACCGCAAC 
TCTGCACTGG TGCTCAACAT GGAGGACCCG TGCATCATCT GCCATGAGGA CATGAGTGCG 
GAGGATCTGT GTGTGCTGGA GTGCCGCCAC AGCTTCCACA GAGAGTGTAT AAAGTCGTGG 
CTGAAGGAGC AGAGCACCTG TCCCACCTGT CGAGAGCATG CGCTGCTGCC GGAGGACTTT 
CCCCTGCTGC CTGGCAGACA ACGCAGAACA CACACCGCCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_009305044.2 ) cDNA ORF clone, -> XP_009303319.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49197
Clone ID Related Accession (Same CDS sequence)	XM_009305044.2 , XM_009305046.2 , XM_009305045.2 , XM_009305043.2
Accession Version	XM_009305044.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303319.1
CDS	123..5210
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGMRAGHSRDEDDEDELFSEEDSLMFLNDMDPFSVPDHLRYQLAEFEENYSGSAQRSYYERMLDNHPDPTKETLYDYFAQVLEEHGPLCATDPLLVGELQNFPLEAQQQIADAGGLQAFLLQSLRFTMTERLIGLMKHAVCLSESSNTHSQILNPSASEFWPLSGEPPSSTGEPALLLPDPYTDEEDQLSAAEMTESDTEETRAEKHTAVRAQDFQDRTDVSVNTEPCAPFENNSGELMGLQKRRLYLLEELQRIRADGEAEQQRRQEELTELQEDTHNISQRMQIASTELSMFQQKLEEEVKKDQQEKKENQETLRSLKMEIKELVELQESLSRDISVRNRQYEAELELFLDVSNQCAAERLSLEDEIKRFRDACVKAERRSASAQLCVLQSRREHALRRLRMTVSEGKMVVKHLSDVALSSPSAALLSAIEDWKRHVSEAEGRIHATEEECEAQMQAVQQGSRLCSLPDISTPSVPSAPALPALQTPVSSAAPEESSSRRSAAPQHLSVYERIVERLHIMFPHYDRLVLNKFLQEVRSSCSGGVLSSLTYNEVINRVAQLILDHQENICQQMNITAGDAADLSCPPSDCTLRAAGTPPLTHVWKNVPEKHRNSALVLNMEDPCIICHEDMSAEDLCVLECRHSFHRECIKSWLKEQSTCPTCREHALLPEDFPLLPGRQRRTHTAS

Target ORF information:

RefSeq Version	XM_009305044.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305044.2

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_009305046.2 ) cDNA ORF clone, -> XP_009303321.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.

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CloneID	ODa49197
Clone ID Related Accession (Same CDS sequence)	XM_009305044.2 , XM_009305046.2 , XM_009305045.2 , XM_009305043.2
Accession Version	XM_009305046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303321.1
CDS	240..5327
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGMRAGHSRDEDDEDELFSEEDSLMFLNDMDPFSVPDHLRYQLAEFEENYSGSAQRSYYERMLDNHPDPTKETLYDYFAQVLEEHGPLCATDPLLVGELQNFPLEAQQQIADAGGLQAFLLQSLRFTMTERLIGLMKHAVCLSESSNTHSQILNPSASEFWPLSGEPPSSTGEPALLLPDPYTDEEDQLSAAEMTESDTEETRAEKHTAVRAQDFQDRTDVSVNTEPCAPFENNSGELMGLQKRRLYLLEELQRIRADGEAEQQRRQEELTELQEDTHNISQRMQIASTELSMFQQKLEEEVKKDQQEKKENQETLRSLKMEIKELVELQESLSRDISVRNRQYEAELELFLDVSNQCAAERLSLEDEIKRFRDACVKAERRSASAQLCVLQSRREHALRRLRMTVSEGKMVVKHLSDVALSSPSAALLSAIEDWKRHVSEAEGRIHATEEECEAQMQAVQQGSRLCSLPDISTPSVPSAPALPALQTPVSSAAPEESSSRRSAAPQHLSVYERIVERLHIMFPHYDRLVLNKFLQEVRSSCSGGVLSSLTYNEVINRVAQLILDHQENICQQMNITAGDAADLSCPPSDCTLRAAGTPPLTHVWKNVPEKHRNSALVLNMEDPCIICHEDMSAEDLCVLECRHSFHRECIKSWLKEQSTCPTCREHALLPEDFPLLPGRQRRTHTAS

Target ORF information:

RefSeq Version	XM_009305046.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305046.2

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_009305045.2 ) cDNA ORF clone, -> XP_009303320.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X4, mRNA.

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CloneID	ODa49197
Clone ID Related Accession (Same CDS sequence)	XM_009305044.2 , XM_009305046.2 , XM_009305045.2 , XM_009305043.2
Accession Version	XM_009305045.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303320.1
CDS	79..5166
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGMRAGHSRDEDDEDELFSEEDSLMFLNDMDPFSVPDHLRYQLAEFEENYSGSAQRSYYERMLDNHPDPTKETLYDYFAQVLEEHGPLCATDPLLVGELQNFPLEAQQQIADAGGLQAFLLQSLRFTMTERLIGLMKHAVCLSESSNTHSQILNPSASEFWPLSGEPPSSTGEPALLLPDPYTDEEDQLSAAEMTESDTEETRAEKHTAVRAQDFQDRTDVSVNTEPCAPFENNSGELMGLQKRRLYLLEELQRIRADGEAEQQRRQEELTELQEDTHNISQRMQIASTELSMFQQKLEEEVKKDQQEKKENQETLRSLKMEIKELVELQESLSRDISVRNRQYEAELELFLDVSNQCAAERLSLEDEIKRFRDACVKAERRSASAQLCVLQSRREHALRRLRMTVSEGKMVVKHLSDVALSSPSAALLSAIEDWKRHVSEAEGRIHATEEECEAQMQAVQQGSRLCSLPDISTPSVPSAPALPALQTPVSSAAPEESSSRRSAAPQHLSVYERIVERLHIMFPHYDRLVLNKFLQEVRSSCSGGVLSSLTYNEVINRVAQLILDHQENICQQMNITAGDAADLSCPPSDCTLRAAGTPPLTHVWKNVPEKHRNSALVLNMEDPCIICHEDMSAEDLCVLECRHSFHRECIKSWLKEQSTCPTCREHALLPEDFPLLPGRQRRTHTAS

Target ORF information:

RefSeq Version	XM_009305045.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305045.2

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_009305043.2 ) cDNA ORF clone, -> XP_009303318.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

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CloneID	ODa49197
Clone ID Related Accession (Same CDS sequence)	XM_009305044.2 , XM_009305046.2 , XM_009305045.2 , XM_009305043.2
Accession Version	XM_009305043.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303318.1
CDS	119..5206
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGMRAGHSRDEDDEDELFSEEDSLMFLNDMDPFSVPDHLRYQLAEFEENYSGSAQRSYYERMLDNHPDPTKETLYDYFAQVLEEHGPLCATDPLLVGELQNFPLEAQQQIADAGGLQAFLLQSLRFTMTERLIGLMKHAVCLSESSNTHSQILNPSASEFWPLSGEPPSSTGEPALLLPDPYTDEEDQLSAAEMTESDTEETRAEKHTAVRAQDFQDRTDVSVNTEPCAPFENNSGELMGLQKRRLYLLEELQRIRADGEAEQQRRQEELTELQEDTHNISQRMQIASTELSMFQQKLEEEVKKDQQEKKENQETLRSLKMEIKELVELQESLSRDISVRNRQYEAELELFLDVSNQCAAERLSLEDEIKRFRDACVKAERRSASAQLCVLQSRREHALRRLRMTVSEGKMVVKHLSDVALSSPSAALLSAIEDWKRHVSEAEGRIHATEEECEAQMQAVQQGSRLCSLPDISTPSVPSAPALPALQTPVSSAAPEESSSRRSAAPQHLSVYERIVERLHIMFPHYDRLVLNKFLQEVRSSCSGGVLSSLTYNEVINRVAQLILDHQENICQQMNITAGDAADLSCPPSDCTLRAAGTPPLTHVWKNVPEKHRNSALVLNMEDPCIICHEDMSAEDLCVLECRHSFHRECIKSWLKEQSTCPTCREHALLPEDFPLLPGRQRRTHTAS

Target ORF information:

RefSeq Version	XM_009305043.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305043.2

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATG AGAGCGGGTC ATTCGCGAGA TGAGGATGAT 
GAAGATGAGC TGTTCTCTGA GGAAGACTCT CTAATGTTCC TGAATGATAT GGATCCGTTC 
AGCGTTCCGG ATCACCTGCG CTATCAGCTG GCGGAGTTTG AGGAGAACTA TAGCGGATCC 
GCACAGCGCA GCTACTACGA GCGCATGCTG GACAACCACC CGGACCCCAC CAAGGAGACT 
TTATATGATT ATTTCGCTCA GGTTCTGGAG GAGCACGGAC CCCTGTGTGC CACGGACCCG 
CTGCTGGTGG GCGAGCTGCA GAACTTCCCT CTGGAGGCGC AGCAGCAGAT CGCAGACGCC 
GGCGGGCTCC AGGCCTTCCT GCTGCAGTCT CTGCGCTTCA CCATGACGGA GCGGCTGATC 
GGGCTGATGA AGCACGCCGT GTGTCTGTCT GAGAGCAGCA ACACACACTC ACAGATCCTC 
AACCCCTCCG CGTCCGAGTT CTGGCCGCTG TCCGGAGAGC CTCCGTCCAG CACAGGTGAG 
CCGGCGCTGC TGCTGCCCGA CCCCTACACT GATGAGGAAG ATCAGCTCTC AGCAGCAGAG 
ATGACCGAGT CCGACACTGA AGAGACGCGA GCTGAGAAAC ACACTGCTGT ACGTGCACAG 
GATTTTCAGG ATCGGACAGA CGTGTCGGTC AACACAGAGC CGTGTGCCCC CTTCGAGAAC 
AACAGTGGTG AGCTGATGGG CTTGCAGAAG CGTCGGCTGT ATCTGCTGGA GGAGCTGCAG 
CGCATCAGGG CTGATGGAGA AGCGGAGCAG CAGAGGAGGC AGGAGGAGCT GACGGAGCTG 
CAGGAGGACA CACACAACAT CAGTCAGAGG ATGCAGATCG CCAGCACGGA GCTCAGCATG 
TTCCAGCAGA AACTGGAGGA GGAGGTGAAG AAAGACCAGC AGGAGAAGAA GGAGAACCAG 
GAGACTCTGA GGAGCCTCAA GATGGAGATC AAGGAGCTGG TGGAGCTGCA GGAGAGCTTG 
AGCAGAGACA TCAGTGTGAG GAACAGACAA TACGAGGCGG AGCTGGAGCT CTTCCTGGAT 
GTTAGTAACC AGTGTGCAGC TGAGCGCTTG AGTCTGGAGG ACGAGATCAA GAGGTTCAGA 
GATGCGTGTG TGAAAGCAGA GCGGAGATCA GCCAGTGCTC AGCTGTGTGT CCTCCAGAGC 
AGGCGTGAAC ATGCGCTGCG GCGTCTTCGC ATGACTGTGT CTGAGGGGAA GATGGTGGTC 
AAACACCTGT CCGACGTGGC CCTCAGTTCC CCCTCGGCTG CGCTGCTGTC CGCCATCGAG 
GACTGGAAGA GACACGTGAG CGAGGCGGAG GGACGCATAC ATGCCACTGA GGAGGAGTGT 
GAGGCTCAGA TGCAGGCGGT GCAGCAGGGC TCCAGACTCT GCTCTCTTCC AGACATCAGC 
ACCCCCAGTG TTCCCTCCGC ACCCGCTCTA CCGGCCCTGC AGACGCCGGT GTCCAGTGCA 
GCTCCAGAAG AGTCCTCCAG CCGCAGGTCT GCAGCGCCGC AGCACCTCAG TGTGTACGAG 
CGCATCGTGG AGAGACTGCA CATCATGTTC CCTCATTACG ACAGGCTGGT GCTGAATAAG 
TTCCTCCAGG AGGTGCGCTC CTCCTGCAGT GGTGGCGTCC TCAGCTCACT GACTTATAAT 
GAGGTGATCA ACCGTGTGGC TCAGCTGATC CTGGACCATC AGGAGAACAT ATGTCAGCAG 
ATGAACATCA CTGCTGGAGA CGCTGCAGAT CTGAGCTGTC CTCCGTCAGA CTGCACACTC 
AGAGCCGCTG GAACTCCACC GCTCACACAC GTCTGGAAGA ACGTCCCGGA GAAACACCGC 
AACTCTGCAC TGGTGCTCAA CATGGAGGAC CCGTGCATCA TCTGCCATGA GGACATGAGT 
GCGGAGGATC TGTGTGTGCT GGAGTGCCGC CACAGCTTCC ACAGAGAGTG TATAAAGTCG 
TGGCTGAAGG AGCAGAGCAC CTGTCCCACC TGTCGAGAGC ATGCGCTGCT GCCGGAGGAC 
TTTCCCCTGC TGCCTGGCAG ACAACGCAGA ACACACACCG CCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTC3 ( XM_009305047.2 ) cDNA ORF clone, -> XP_009303322.1 Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X6, mRNA.

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CloneID	ODa49198
Clone ID Related Accession (Same CDS sequence)	XM_009305047.2
Accession Version	XM_009305047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase TTC3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATA ACCTTGTGTG TGTGTGTGTG TGTGCGTGTG 
TGCGTGTGTG TTTCAGTCTG TTATTGTGCT GTCTGGAATA ACCGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTT TCAGTCTGTT ATTGTGCTGT CTGGAATAAC CGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGCGTGTGTG TGTGCGTGTG TGTTTCAGTC 
TGTTATTGTG CTGTCTGGAA TAACCTTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTTTCAGTC TGTTATTGTG CTGTCTGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303322.1
CDS	344..3676
Translation	MSDSDDSDLEEGPNVQRHKTKIVYHAQFPYIHMEPPDIVYERWCKMKLKRKQDLGHLMHVSVFWWHILLRQQENHPTTAWAVKMGFLDSNVSNDLSLKRLEKMEILESILATMERGLSLRDVENKARAVVSLSQRFFQEEDCLTEAIRWLLDSGDPDLGQKLMDLGSVEVRYLTLSFVFMEYARYIQVVSCSKKKALMEMKFEPDSWSLSKSDEMKNKGNEHFQKKKYDVALKWYSKAIKYHPNNHILYGNRALCLLRSGKYLKALGDGKRAIVLQPDWAKGHYRFCDALFYLGEHQRALLANQQAQDACRADVEGQKDLLQQHERFQSELQEARDMKTKKPDPKKVSSKARSHTAGASSAAHLKTSAPPETPPEPQTKKSEKQKQQADTSGPSGARAHTDGGADRLRDVSSESSPRKAKKASSSTAEKPAVRSKLPAAADRASAPAEVVSAAAVRERFCAAVLEAHVALSDQRCHNAQQSFSVALAILESSSTTELGLTELDKSVLLYGYATALLETGQPEELAEAEKLFAKLESTERKFQSLVHYGMGRVYLKENRFAKARECFSNALLMIQRHITPGKLTWPTTKAIVEETQPAWLQEHLESLVEVCRFPPKADAVCRHQQCVSHSVQIYFSDPDFKGFIQLVCCQSCRIEFHMSCWRKLKTLSFADKNDKDFLKDSCFTPDCAGQICHILIFGSTGLIKCEFKSSIPKARAAGRSRLRQQCTSIKKLMSKNDRKLRRKQQRLAARSSHQEKREVMREEAAKEQPADRASPRDCIYMDRVLQQIHDNKALFKEEPVSISCFWEHLRPWLDLFESKGQESVLNSRRGVGVLSELVDLLLESRNRVWARVFIQTLGSTLHIKPRLQHWAQQLDDTGLKAAQSFINRYRSHLEDLELKPLLSFPPLQESLVEKLGSVTELFDRDDFTVMEYLRQAPAKEMRLFIWTLESSRERYQSCHTILDQYFEDDAVCLVIKKTDDDEHMSSVFKSKNRHRKKKHKELKSVIVLSGITLCVCVCVRVCVCVSVCYCAVWNNRVCVCVCVCVCVFQSVIVLSGITVCVCVCVCVCVCVCVCVCVCVSVCYCAVWNNLVCVCVCVCVCVCFSLLLCCLE

Target ORF information:

RefSeq Version	XM_009305047.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tetratricopeptide repeat domain 3 (ttc3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305047.2

ORF Insert Sequence:

ATGTCAGACA GTGACGACTC AGATCTGGAG GAAGGACCCA ATGTTCAGAG GCACAAAACC 
AAGATTGTCT ACCATGCTCA GTTCCCCTAC ATCCACATGG AGCCGCCGGA CATCGTGTAC 
GAGCGCTGGT GCAAGATGAA GCTGAAGCGG AAGCAGGACC TGGGTCACCT GATGCACGTG 
TCTGTGTTCT GGTGGCACAT TCTGCTCCGC CAGCAGGAGA ACCACCCCAC CACCGCCTGG 
GCCGTCAAGA TGGGCTTCCT GGACTCTAAC GTGTCCAACG ATTTGAGCCT GAAGAGGCTT 
GAGAAGATGG AGATTCTGGA GAGCATTCTG GCGACGATGG AGAGAGGCCT TTCTCTTAGA 
GACGTGGAGA ACAAGGCCAG AGCCGTGGTG TCCTTGAGCC AGCGCTTCTT TCAGGAGGAG 
GACTGTCTGA CTGAGGCCAT ACGCTGGCTG CTGGACTCTG GAGATCCTGA CCTGGGCCAG 
AAACTGATGG ATCTGGGCTC GGTGGAGGTT CGCTACCTAA CACTGAGCTT CGTCTTCATG 
GAGTACGCGC GCTATATTCA GGTGGTGTCC TGCAGCAAGA AGAAAGCCTT GATGGAGATG 
AAATTTGAGC CAGACTCTTG GAGTTTATCG AAAAGTGATG AAATGAAGAA CAAAGGAAAC 
GAGCACTTTC AGAAGAAGAA ATATGACGTT GCCTTAAAGT GGTACTCTAA AGCCATTAAA 
TACCACCCGA ATAACCACAT CCTGTACGGG AACCGAGCGC TGTGTTTACT CCGCTCTGGG 
AAATATCTGA AAGCACTCGG AGATGGAAAG AGAGCCATTG TCTTACAGCC GGACTGGGCC 
AAGGGTCATT ACCGGTTCTG TGACGCGCTC TTCTACCTGG GCGAGCACCA GAGGGCTCTG 
CTGGCCAACC AGCAGGCTCA GGACGCCTGC AGAGCAGATG TGGAGGGACA GAAAGACCTG 
CTGCAGCAGC ACGAGCGCTT CCAGAGCGAA CTGCAGGAGG CCAGAGACAT GAAGACTAAG 
AAGCCGGACC CAAAGAAGGT TTCCTCTAAA GCGAGGTCAC ACACTGCCGG CGCGAGTTCA 
GCTGCGCATC TGAAGACCAG CGCTCCGCCC GAAACACCAC CAGAGCCGCA GACTAAGAAA 
TCTGAAAAGC AGAAACAGCA GGCGGACACT TCAGGGCCTT CAGGAGCTCG AGCACACACT 
GACGGAGGAG CAGACAGACT GCGAGACGTC AGCAGTGAAT CGAGTCCTCG CAAGGCTAAA 
AAAGCTTCAT CCAGCACTGC AGAGAAGCCG GCGGTGCGGA GCAAACTACC AGCGGCTGCA 
GACCGAGCCA GCGCACCTGC GGAGGTGGTG AGCGCAGCAG CAGTGCGGGA GCGTTTCTGT 
GCCGCTGTAC TGGAGGCTCA CGTGGCTCTG AGTGACCAGC GCTGCCACAA CGCTCAGCAG 
TCCTTCAGTG TGGCGCTCGC GATCCTGGAG TCCAGCAGCA CCACGGAGCT GGGCTTGACG 
GAGCTGGATA AATCTGTGCT CCTGTATGGA TATGCCACAG CTCTGCTGGA GACCGGACAG 
CCTGAGGAGT TGGCTGAAGC AGAGAAGTTA TTCGCCAAAT TAGAGTCGAC AGAGAGGAAG 
TTTCAGTCTC TGGTGCATTA TGGGATGGGC AGAGTGTATC TGAAGGAGAA CAGGTTTGCT 
AAAGCGCGTG AGTGTTTCTC TAATGCGCTG CTGATGATCC AGAGACACAT CACACCTGGA 
AAACTCACCT GGCCCACCAC CAAAGCTATA GTGGAGGAGA CACAGCCGGC CTGGCTACAG 
GAGCACCTGG AGAGCCTGGT GGAGGTGTGC AGGTTTCCTC CTAAAGCAGA CGCTGTGTGT 
CGTCATCAGC AGTGTGTGTC CCACAGCGTC CAGATCTACT TCAGCGACCC TGACTTTAAG 
GGGTTCATCC AGCTGGTGTG TTGTCAGAGC TGCAGAATCG AGTTTCATAT GAGCTGCTGG 
AGGAAGCTCA AAACACTTTC ATTCGCTGAT AAGAATGACA AGGATTTTCT AAAAGATTCA 
TGTTTTACGC CAGACTGCGC TGGGCAGATC TGTCACATCC TAATATTTGG CTCGACAGGG 
CTGATAAAGT GTGAGTTTAA ATCATCTATT CCTAAAGCCA GAGCTGCGGG ACGCTCCAGA 
CTCCGGCAGC AGTGTACGAG TATTAAGAAG TTGATGTCTA AAAATGATCG TAAACTTCGG 
CGGAAGCAGC AGAGATTGGC GGCGCGTTCA TCCCATCAGG AGAAAAGAGA AGTAATGCGT 
GAGGAAGCAG CTAAAGAGCA GCCGGCGGAC AGAGCATCAC CCAGAGACTG TATTTATATG 
GACCGTGTTT TACAACAAAT TCATGATAAC AAAGCACTTT TTAAAGAAGA GCCAGTGAGC 
ATTTCCTGTT TTTGGGAGCA TCTGCGGCCG TGGCTGGACT TGTTTGAGAG TAAAGGACAG 
GAGAGCGTGT TAAACAGCCG TAGAGGTGTT GGGGTGCTGT CGGAGCTGGT GGATCTGCTG 
CTGGAGTCCA GAAACCGGGT CTGGGCTCGA GTGTTTATTC AGACGCTGGG CTCCACGCTT 
CATATCAAAC CCAGACTGCA GCACTGGGCT CAGCAGCTGG ACGACACCGG TCTGAAGGCC 
GCTCAGTCAT TCATCAACCG CTATCGATCT CACCTGGAGG ATCTGGAGCT GAAGCCTCTG 
CTCTCCTTCC CGCCGCTGCA GGAGTCGCTC GTTGAGAAAT TGGGCAGCGT TACGGAGTTA 
TTTGACCGTG ACGATTTTAC TGTAATGGAG TACTTAAGAC AAGCTCCCGC TAAAGAAATG 
CGTCTGTTTA TTTGGACTCT AGAGTCGAGT CGAGAGCGAT ATCAGAGCTG TCACACCATT 
CTCGATCAAT ATTTTGAAGA CGATGCTGTT TGTTTAGTCA TTAAAAAGAC AGATGATGAT 
GAGCACATGA GTTCAGTATT TAAGAGTAAA AACAGACATC GCAAAAAGAA GCATAAGGAA 
TTAAAGTCTG TTATTGTGCT GTCTGGAATA ACCTTGTGTG TGTGTGTGTG TGTGCGTGTG 
TGCGTGTGTG TTTCAGTCTG TTATTGTGCT GTCTGGAATA ACCGTGTGTG TGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTT TCAGTCTGTT ATTGTGCTGT CTGGAATAAC CGTGTGTGTG 
TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG TGCGTGTGTG TGTGCGTGTG TGTTTCAGTC 
TGTTATTGTG CTGTCTGGAA TAACCTTGTG TGTGTGTGTG TGTGTGTGTG TGTGTGTGTG 
TGTTTCAGTC TGTTATTGTG CTGTCTGGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Gene Symbol	TTC3
Entrez Gene ID	100329450

TTC3 cDNA ORF clone,

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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