hlcs cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hlcs
Entrez Gene ID	100492512
Full Name	holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase)
General protein information	Preferred Names holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) Names biotin--protein ligase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912115.3	XP_004912172.1	biotin--protein ligase isoform X1
XM_002941531.4	XP_002941577.2	biotin--protein ligase isoform X2
XM_004912117.3	XP_004912174.1	biotin--protein ligase isoform X1
XM_004912116.3	XP_004912173.1	biotin--protein ligase isoform X1
XM_004912116.4	XP_004912173.1	biotin--protein ligase
XM_004912115.4	XP_004912172.1	biotin--protein ligase
XM_004912117.4	XP_004912174.1	biotin--protein ligase

Pathways from BioSystems

Homologies

Homo sapiens (human)	HLCS	NP_000402.3
Arabidopsis thaliana (thale cress)	HCS1	NP_565605.1
Xenopus tropicalis (tropical clawed frog)	hlcs	XP_004912175.1
Pan troglodytes (chimpanzee)	HLCS	XP_003319106.2
	HLCS	XP_001084173.2
Bos taurus (cattle)	HLCS	XP_005202184.1
Arabidopsis thaliana (thale cress)	HCS2	NP_850957.1
Mus musculus (house mouse)	Hlcs	NP_631884.1
Gallus gallus (chicken)	HLCS	XP_004934596.1
Canis lupus familiaris (dog)	HLCS	XP_005638959.1
Rattus norvegicus (Norway rat)	Hlcs	XP_006248175.1
Caenorhabditis elegans (roundworm)	bpl-1	NP_871920.1
Danio rerio (zebrafish)	hlcs	XP_002663496.3

Gene Ontology

The following hlcs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hlcs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06403	XM_002941531.4 Latest version!	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa06402	XM_004912117.4 Latest version!	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa06402	XM_004912116.4 Latest version!	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa06402	XM_004912115.4 Latest version!	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa06402	XM_004912116.3	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa06402	XM_004912115.3	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa06402	XM_004912117.3	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hlcs ( XM_004912115.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912172.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912172.1
CDS	323..2884
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912115.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912115.3

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_004912117.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912174.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912117.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912174.1
CDS	278..2839
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912117.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912117.3

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_004912116.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912173.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X2, mRNA.

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CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912116.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912116.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912173.1
CDS	318..2879
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912116.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912116.3

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_002941531.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941577.2 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa06403
Clone ID Related Accession (Same CDS sequence)	XM_002941531.4
Accession Version	XM_002941531.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941531.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GACTGCTGAT GGACAATGGG TCACCGCTCC ATAAAATCAC ATCTGTATAT 
CTAAAGGAGT CGGCTCTGAA GGCCCAAGAA ACAGAGGAGA CAAATCTCAG AACCTCTAAG 
AGCAGAACTG AACCGCATTT GGCTCCCTCA GTTGGACAGG AAAATATGGG AACGGCTGAG 
AGCAGTGCCG CCACTGATAA AGGCGAGGAG CTGGAAGCCC ACCGTCATCT CCATCTGTCA 
AGTTGCCACG AGTGCTTGGA ACTGGAGAAC CGCACCATAG AGTCCGTGCG CTGTGCCTCT 
GAAGAGAACA TTCCTGACCT CCCCGAGGAT TTCAGCAGCT TAGAAGGCAG TGAATTACAG 
AACCACGGCA GCCTTAACGT GTCGGGGAAA CCACCCAACG TCCTTATATA CTGCGGCCCT 
GAAACCGGCG GCCAATTCCA AGAAATCAAG TCTGTTCTCC TACAGTGTTT CGATTCCTCT 
AGGTATGTCA TTTATCCTCT TCCTGATGAT CAGGTTCTGA AAGCTCCGTG GACAGATAAC 
TGCTTGTTAT TGGTGGTGGC CTCCGACCAT CCAGTCTCAA ATCACATTGA AGAGCAGTTC 
TTGAGTTATT TGTCCAAAGG TGGAAAGATT TTGGGACTTT CCTCCTCCTC CTCCTTCGCC 
TTCGGCAGTG TATCGGTAAC GAGCAAAAGC CAACTGCAGG GGTCTGTCCA GGCTCTGGTT 
TTCGGGAGAC CCAACTGTGA CCCGGTCAGT TTGGACTTGG TGGCCAGCGG GAAAGTATTT 
GCGGAACTTT CTAGTGAAGC ATCACCGCAA GTGTCAGGGT GGGGGTATTT AAACAACGAC 
GACAAAGACT TGATTCTGGT TCACCAGACC TATGGTGATA ATGGCGGGGA AGCTGTTCTT 
TGCCAGGTAA AACTTGAAGC CTCTCCAGAG TCTGTCAGTG AGGACGACAC AGGGACCTTT 
GAGTCACTTA AGAAAAGCAA CCCCCTGAGA TACGCCGTCC TGACCCAGAT CCTCATCAGC 
CTTGGCCTGG ATTGTGACTT GGCCTCACCT CCAGCTCTCA CTCCTGTTTA TTTACTCACT 
GCTGAACAGA CTTGTTATGA TGACGTTCTC CATTGGCTCA AAACCCGAAC GGACAGCGGT 
GGCTTGATAA AGTCTGGCAA GATGTCTCTA AAAGTCTGCC CTTCACCCCA GCCCGACATA 
GACGTCAGCC CAACCCTGTC ACCTCTTGTA ACCCAACCGG AAAGCTTCAC ATGTGAAGAA 
TTCAGCGTAG AGAGGTACAG CGAAAGCCTC TGCACGGACA AGCTTGGCAA AATCGTGCTT 
TTTGCTGAAG TTACCACCAC AACCTTCAAC CTACTGGAAG GGCTGATGTT TCACGAACCC 
AAGGAAATGG GGTTAATTGC CATCGCAGCT CATCAGACTC AAGGCAAAGG TCGAGGCGGC 
AATGCGTGGC TCAGCCCTAA GGGATGTGCC TTAATGACGT TGCACATCTC AATCCCGTTG 
CTGTCTGAGC TGGGCCAGAG GATCGCTTTT GTACAACACC TGATGTCCCT GGCGGTGGTG 
GAATCTGTGA GGTCCTTACC TGGATATGAG GACATTGACC TGAGAGTAAA ATGGCCCAAC 
GACATTTACT ATGGCGATTT GATGAAGATT GGTGGAGTTC TGGTGAATTC TACACTTATG 
GGAAACACTT TTCACATTCT AATTGGCTGC GGGTTCAACG TCAGCAATAG TAACCCAACT 
GTTTGCATCA ACGATCTCAT CGCGCAGCAT AACAAGACCT ATAAAACAAA TATTGAACCA 
CTGCGGGTGG ACACTTTGAT CGCCAGGGCT GTGACGGCAT TAGAAGGACT CATAAAGGCA 
TTTCAGAGCA AAGGACCCGA CGGTGTCCTA CCTGCTTATT ATAAATATTG GCTTCACAGT 
GGTGCTAAGG TCCGGCTGGG TGGGGACGAT GGTCCTTTGG CATGGATAAT TGGAGTCGAT 
GATTCCGGAT TCCTTCAGGT TCTACAGGAA GGGAAGGAAA TTGTCTCAGT GCATCCTGAT 
GGCAACTCTT TTGACATGCT CAGAAACCTC ATTATACCAA AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941577.2
CDS	318..2405
Translation	MEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_002941531.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941531.4

ORF Insert Sequence:

ATGGAAGACA GACTGCTGAT GGACAATGGG TCACCGCTCC ATAAAATCAC ATCTGTATAT 
CTAAAGGAGT CGGCTCTGAA GGCCCAAGAA ACAGAGGAGA CAAATCTCAG AACCTCTAAG 
AGCAGAACTG AACCGCATTT GGCTCCCTCA GTTGGACAGG AAAATATGGG AACGGCTGAG 
AGCAGTGCCG CCACTGATAA AGGCGAGGAG CTGGAAGCCC ACCGTCATCT CCATCTGTCA 
AGTTGCCACG AGTGCTTGGA ACTGGAGAAC CGCACCATAG AGTCCGTGCG CTGTGCCTCT 
GAAGAGAACA TTCCTGACCT CCCCGAGGAT TTCAGCAGCT TAGAAGGCAG TGAATTACAG 
AACCACGGCA GCCTTAACGT GTCGGGGAAA CCACCCAACG TCCTTATATA CTGCGGCCCT 
GAAACCGGCG GCCAATTCCA AGAAATCAAG TCTGTTCTCC TACAGTGTTT CGATTCCTCT 
AGGTATGTCA TTTATCCTCT TCCTGATGAT CAGGTTCTGA AAGCTCCGTG GACAGATAAC 
TGCTTGTTAT TGGTGGTGGC CTCCGACCAT CCAGTCTCAA ATCACATTGA AGAGCAGTTC 
TTGAGTTATT TGTCCAAAGG TGGAAAGATT TTGGGACTTT CCTCCTCCTC CTCCTTCGCC 
TTCGGCAGTG TATCGGTAAC GAGCAAAAGC CAACTGCAGG GGTCTGTCCA GGCTCTGGTT 
TTCGGGAGAC CCAACTGTGA CCCGGTCAGT TTGGACTTGG TGGCCAGCGG GAAAGTATTT 
GCGGAACTTT CTAGTGAAGC ATCACCGCAA GTGTCAGGGT GGGGGTATTT AAACAACGAC 
GACAAAGACT TGATTCTGGT TCACCAGACC TATGGTGATA ATGGCGGGGA AGCTGTTCTT 
TGCCAGGTAA AACTTGAAGC CTCTCCAGAG TCTGTCAGTG AGGACGACAC AGGGACCTTT 
GAGTCACTTA AGAAAAGCAA CCCCCTGAGA TACGCCGTCC TGACCCAGAT CCTCATCAGC 
CTTGGCCTGG ATTGTGACTT GGCCTCACCT CCAGCTCTCA CTCCTGTTTA TTTACTCACT 
GCTGAACAGA CTTGTTATGA TGACGTTCTC CATTGGCTCA AAACCCGAAC GGACAGCGGT 
GGCTTGATAA AGTCTGGCAA GATGTCTCTA AAAGTCTGCC CTTCACCCCA GCCCGACATA 
GACGTCAGCC CAACCCTGTC ACCTCTTGTA ACCCAACCGG AAAGCTTCAC ATGTGAAGAA 
TTCAGCGTAG AGAGGTACAG CGAAAGCCTC TGCACGGACA AGCTTGGCAA AATCGTGCTT 
TTTGCTGAAG TTACCACCAC AACCTTCAAC CTACTGGAAG GGCTGATGTT TCACGAACCC 
AAGGAAATGG GGTTAATTGC CATCGCAGCT CATCAGACTC AAGGCAAAGG TCGAGGCGGC 
AATGCGTGGC TCAGCCCTAA GGGATGTGCC TTAATGACGT TGCACATCTC AATCCCGTTG 
CTGTCTGAGC TGGGCCAGAG GATCGCTTTT GTACAACACC TGATGTCCCT GGCGGTGGTG 
GAATCTGTGA GGTCCTTACC TGGATATGAG GACATTGACC TGAGAGTAAA ATGGCCCAAC 
GACATTTACT ATGGCGATTT GATGAAGATT GGTGGAGTTC TGGTGAATTC TACACTTATG 
GGAAACACTT TTCACATTCT AATTGGCTGC GGGTTCAACG TCAGCAATAG TAACCCAACT 
GTTTGCATCA ACGATCTCAT CGCGCAGCAT AACAAGACCT ATAAAACAAA TATTGAACCA 
CTGCGGGTGG ACACTTTGAT CGCCAGGGCT GTGACGGCAT TAGAAGGACT CATAAAGGCA 
TTTCAGAGCA AAGGACCCGA CGGTGTCCTA CCTGCTTATT ATAAATATTG GCTTCACAGT 
GGTGCTAAGG TCCGGCTGGG TGGGGACGAT GGTCCTTTGG CATGGATAAT TGGAGTCGAT 
GATTCCGGAT TCCTTCAGGT TCTACAGGAA GGGAAGGAAA TTGTCTCAGT GCATCCTGAT 
GGCAACTCTT TTGACATGCT CAGAAACCTC ATTATACCAA AGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_004912116.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912173.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.

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CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912116.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912116.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912173.1
CDS	312..2873
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912116.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912116.4

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_004912115.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912172.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912115.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912172.1
CDS	317..2878
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912115.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912115.4

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hlcs ( XM_004912117.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912174.1 Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.

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CloneID	OXa06402
Clone ID Related Accession (Same CDS sequence)	XM_004912115.3 , XM_004912117.3 , XM_004912116.3 , XM_004912116.4 , XM_004912115.4 , XM_004912117.4
Accession Version	XM_004912117.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2562bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	biotin--protein ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912117.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912174.1
CDS	272..2833
Translation	MLITLCYMYLWLRFRSSYAVLIRSCVHRLHRTNSFTFCTQRQGRKSLRGTQEPKEDKFSLKVGRKLFLIEGSQLFEDLNKWNLLLESPRDSDLGKGQITFITESVSLPSETLSASSNSSKKVQKLSEYCLLLARYHEDPCKLLAEASVEDFSELGIAFMEDRLLMDNGSPLHKITSVYLKESALKAQETEETNLRTSKSRTEPHLAPSVGQENMGTAESSAATDKGEELEAHRHLHLSSCHECLELENRTIESVRCASEENIPDLPEDFSSLEGSELQNHGSLNVSGKPPNVLIYCGPETGGQFQEIKSVLLQCFDSSRYVIYPLPDDQVLKAPWTDNCLLLVVASDHPVSNHIEEQFLSYLSKGGKILGLSSSSSFAFGSVSVTSKSQLQGSVQALVFGRPNCDPVSLDLVASGKVFAELSSEASPQVSGWGYLNNDDKDLILVHQTYGDNGGEAVLCQVKLEASPESVSEDDTGTFESLKKSNPLRYAVLTQILISLGLDCDLASPPALTPVYLLTAEQTCYDDVLHWLKTRTDSGGLIKSGKMSLKVCPSPQPDIDVSPTLSPLVTQPESFTCEEFSVERYSESLCTDKLGKIVLFAEVTTTTFNLLEGLMFHEPKEMGLIAIAAHQTQGKGRGGNAWLSPKGCALMTLHISIPLLSELGQRIAFVQHLMSLAVVESVRSLPGYEDIDLRVKWPNDIYYGDLMKIGGVLVNSTLMGNTFHILIGCGFNVSNSNPTVCINDLIAQHNKTYKTNIEPLRVDTLIARAVTALEGLIKAFQSKGPDGVLPAYYKYWLHSGAKVRLGGDDGPLAWIIGVDDSGFLQVLQEGKEIVSVHPDGNSFDMLRNLIIPKK

Target ORF information:

RefSeq Version	XM_004912117.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase) (hlcs), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912117.4

ORF Insert Sequence:

ATGCTCATTA CCCTCTGCTA CATGTATCTG TGGCTGCGAT TCAGATCCTC ATATGCTGTG 
CTGATCCGCA GCTGTGTGCA TCGGCTGCAC AGGACCAACT CATTCACTTT CTGTACTCAG 
CGGCAGGGGA GAAAGAGCCT TAGAGGAACC CAAGAGCCAA AGGAAGATAA GTTCTCTCTG 
AAAGTGGGCA GAAAGCTCTT CCTCATTGAG GGTTCCCAGC TCTTTGAAGA TCTGAACAAA 
TGGAATCTCC TCCTTGAATC CCCTAGAGAT AGTGATCTGG GAAAAGGACA GATTACCTTT 
ATCACTGAGA GCGTCTCTTT ACCAAGTGAA ACCTTATCAG CCTCATCCAA CTCTTCCAAA 
AAGGTTCAGA AGCTGTCCGA ATACTGTTTG CTTCTGGCTC GGTACCATGA AGATCCCTGC 
AAGCTGCTTG CGGAAGCCAG TGTAGAAGAC TTCAGTGAGC TCGGCATAGC GTTTATGGAA 
GACAGACTGC TGATGGACAA TGGGTCACCG CTCCATAAAA TCACATCTGT ATATCTAAAG 
GAGTCGGCTC TGAAGGCCCA AGAAACAGAG GAGACAAATC TCAGAACCTC TAAGAGCAGA 
ACTGAACCGC ATTTGGCTCC CTCAGTTGGA CAGGAAAATA TGGGAACGGC TGAGAGCAGT 
GCCGCCACTG ATAAAGGCGA GGAGCTGGAA GCCCACCGTC ATCTCCATCT GTCAAGTTGC 
CACGAGTGCT TGGAACTGGA GAACCGCACC ATAGAGTCCG TGCGCTGTGC CTCTGAAGAG 
AACATTCCTG ACCTCCCCGA GGATTTCAGC AGCTTAGAAG GCAGTGAATT ACAGAACCAC 
GGCAGCCTTA ACGTGTCGGG GAAACCACCC AACGTCCTTA TATACTGCGG CCCTGAAACC 
GGCGGCCAAT TCCAAGAAAT CAAGTCTGTT CTCCTACAGT GTTTCGATTC CTCTAGGTAT 
GTCATTTATC CTCTTCCTGA TGATCAGGTT CTGAAAGCTC CGTGGACAGA TAACTGCTTG 
TTATTGGTGG TGGCCTCCGA CCATCCAGTC TCAAATCACA TTGAAGAGCA GTTCTTGAGT 
TATTTGTCCA AAGGTGGAAA GATTTTGGGA CTTTCCTCCT CCTCCTCCTT CGCCTTCGGC 
AGTGTATCGG TAACGAGCAA AAGCCAACTG CAGGGGTCTG TCCAGGCTCT GGTTTTCGGG 
AGACCCAACT GTGACCCGGT CAGTTTGGAC TTGGTGGCCA GCGGGAAAGT ATTTGCGGAA 
CTTTCTAGTG AAGCATCACC GCAAGTGTCA GGGTGGGGGT ATTTAAACAA CGACGACAAA 
GACTTGATTC TGGTTCACCA GACCTATGGT GATAATGGCG GGGAAGCTGT TCTTTGCCAG 
GTAAAACTTG AAGCCTCTCC AGAGTCTGTC AGTGAGGACG ACACAGGGAC CTTTGAGTCA 
CTTAAGAAAA GCAACCCCCT GAGATACGCC GTCCTGACCC AGATCCTCAT CAGCCTTGGC 
CTGGATTGTG ACTTGGCCTC ACCTCCAGCT CTCACTCCTG TTTATTTACT CACTGCTGAA 
CAGACTTGTT ATGATGACGT TCTCCATTGG CTCAAAACCC GAACGGACAG CGGTGGCTTG 
ATAAAGTCTG GCAAGATGTC TCTAAAAGTC TGCCCTTCAC CCCAGCCCGA CATAGACGTC 
AGCCCAACCC TGTCACCTCT TGTAACCCAA CCGGAAAGCT TCACATGTGA AGAATTCAGC 
GTAGAGAGGT ACAGCGAAAG CCTCTGCACG GACAAGCTTG GCAAAATCGT GCTTTTTGCT 
GAAGTTACCA CCACAACCTT CAACCTACTG GAAGGGCTGA TGTTTCACGA ACCCAAGGAA 
ATGGGGTTAA TTGCCATCGC AGCTCATCAG ACTCAAGGCA AAGGTCGAGG CGGCAATGCG 
TGGCTCAGCC CTAAGGGATG TGCCTTAATG ACGTTGCACA TCTCAATCCC GTTGCTGTCT 
GAGCTGGGCC AGAGGATCGC TTTTGTACAA CACCTGATGT CCCTGGCGGT GGTGGAATCT 
GTGAGGTCCT TACCTGGATA TGAGGACATT GACCTGAGAG TAAAATGGCC CAACGACATT 
TACTATGGCG ATTTGATGAA GATTGGTGGA GTTCTGGTGA ATTCTACACT TATGGGAAAC 
ACTTTTCACA TTCTAATTGG CTGCGGGTTC AACGTCAGCA ATAGTAACCC AACTGTTTGC 
ATCAACGATC TCATCGCGCA GCATAACAAG ACCTATAAAA CAAATATTGA ACCACTGCGG 
GTGGACACTT TGATCGCCAG GGCTGTGACG GCATTAGAAG GACTCATAAA GGCATTTCAG 
AGCAAAGGAC CCGACGGTGT CCTACCTGCT TATTATAAAT ATTGGCTTCA CAGTGGTGCT 
AAGGTCCGGC TGGGTGGGGA CGATGGTCCT TTGGCATGGA TAATTGGAGT CGATGATTCC 
GGATTCCTTC AGGTTCTACA GGAAGGGAAG GAAATTGTCT CAGTGCATCC TGATGGCAAC 
TCTTTTGACA TGCTCAGAAA CCTCATTATA CCAAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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hlcs cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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