ankrd50 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following ankrd50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankrd50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa41951 XM_018096153.1
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa48105 XM_018096150.2
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48105 XM_002941998.5
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48105 XM_031902563.1
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa04555 XM_018096150.1
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa04555 XM_002941998.4
Latest version!
Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa41951
    Clone ID Related Accession (Same CDS sequence) XM_018096153.1
    Accession Version XM_018096153.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCCTTC TGTTCAGAGC CTGTTGCAGC CAGGAGAATG TGAACGGAAC CCTTCAGAGG 
    CATTTAAAAG GTTTCCGAAA AATAAGTCTG GATGATCTCC GGAAAGCTTA CATAGTAAAA
    GATGTTCAAC AGTATATTCT TCATCGTTTG GACCAAGAAG AGGCATTGAG GCAACACCTA
    ACAAAAGAAA CTGCGGAAAT GTTAAATCAA CTGCACATTA AAAGCAGTGG GTGCTTTCTG
    TACCTGGAGC GTGTTTTAGA TGGAGTTGTA GAAAATTTCA TCATGCTACG AGAGATCCGT
    GATATTCCTG GAACTCTAAA TGGCTTATAC CTCTGGTTGT GTCAAAGACT TTTTGTTAGA
    AAACAATTTG CAAAGGTCCA ACCAATTCTT AATGTTATTC TGGCTGCTTG TAAGCCATTA
    ACAACTATTG AACTCTTTAA TGCAGTTTGG ACCAAAAACA TGTCATTAAC CATGGAAGAT
    TTTCAGAGGA AACTGGATGT GCTATCAAAA GTCTTGGTCG AAGGATTGGG GAATACTAAA
    ATTTTATTCC ATTATAGTTT TGCAGAGTGG CTGCTAGATG TAAAACATTG CACACAGAAA
    TATTTGTGTA ATGCAGCTGA AGGACACCGA ATGCTTGCAA TGAGCTACAC ATGTCGGGCT
    AAGGAGCTTA CGCCAATAGA AGTACAGGAG TTTGCTTTGC ATCTTATACA TTCAAATTTA
    CAGTTAACCA ATTCAGAGTT GGCTTTATGG ATGATTTTGA ATGGAACATG TGTGAAAGAC
    TCATTGTGCA CCTTAATACC AAAGGAACAA GAAGTACTAC AGTTGTTGGT AAAAGCAGGA
    GCCCATGTTG ATAGTGAGGA TGATAGAACA TGTTGCATTG TTCGACAAGC ATTGGAACGA
    GAAGATTCTA TTCGTACGTT GCTGGATAAT GGTGCTTCAG TTAACCAGTG TGATTCAAAT
    GGGAGAACTT TGCTGGCCAA CACTGCTTAT AGTGGAAATC TTGATGTGGT TAACCTTCTT
    GTTTCAAGAG GATCTGATCT TGAAATAGAA GATGCCAATG GACAGACAGC ACTTACTTTA
    GCTGCCCGCC AAGGACATGT AAAGGTTGTA AATTGTTTAA TTGGATGCGG TGCTAATATA
    AATCATTGTG ATCATGATGG ATGGACTGCA CTCAGATCTG CAGCCTGGGG TGGGCACACT
    GAAGTAGTGT CTGCACTTCT GTATGCAGGT GCAAAGGTAG ATTGTGCTGA TGCAGATAGT
    AGAACAGCTT TAAGAGCAGC AGCATGGGGT GGGCATGAAG ATATTGTGCT GAATTTACTG
    CAGCATGGAG CAGAGGTTAA TAAAGCTGAC AATGAGGGAC GAACTGCTTT AATTGCTGCA
    GCATATATGG GACATAGAGA AATTGTGGAG CATCTTTTAG GTCATGGTGC AGAGATAAAC
    CATGAGGATG TGGATGGAAG AACTGCTTTA TCAGTTGCTG CTCTTTGTGT ACCTGCAAGC
    AAAGGGCATG CATCCGTTGT TAGTCTTTTA ATTGAACAAG GTGCTGAGGT GGATCATTGT
    GATAAGGATG GAATGACTCC ACTACTTGTT GCAGCATATG AAGGCCATGT AGATGTAGTT
    GACTTACTTC TAGAGGGTGG AGCAGATGTC GATCATACTG ATAATAATGG ACGTACTCCT
    CTGCTTGCTG CTGCTTCAAT GGGGCATGCA GCAGTTGTCA ACACACTTCT GTTTTGGGGT
    GCTGCTGTTG ATAGCATTGA TAGTGAAGGT AGAACTGTGC TTAGTATAGC TTCAGCTCAA
    GGCAATGTTG AAGTTGTGCG GACACTTTTA GACCGGGGCC TTGATGAAAA TCATAGAGAT
    GATGCTGGAT GGACACCTCT GCATATGGCT GCTTTTGAAG GTCATAGATT AATCTGTGAA
    GCATTAATTG AACAAGGGGC TAGAACAAAT GAAATTGATA ATGATGGACG AATACCTCTG
    ATTCTGGCTG CACAAGAAGG GCACTATGAT TGTGTGCAAA TACTTCTAGA AAATAAATCT
    GCAGTGGATC AAAAAGGCTA TGATGGCCGA AGTGCCTTGA GAGTTGCAGC ACTGGAGGGA
    CACAGAGACA TAGTTGAATT GCTTCTCAGT AATGGAGCAG ATCTTAATGC CAAAGATGCC
    GATGGTCGAC CAACACTATA CATACTGGCC CTCGAAAATC AGCTCTCGAT GGCTGAGTAT
    TTTTTAGAAA ATGGAGCAAA TGTAGAAGCT AGCGATACTG ATGGAAGGAC AGCTCTTCAT
    GTCTCCTGCT GGCAGGGACA TTTAGAAATG GTGCAGAGTC TAATATCCTA TAAGGCTGAT
    GTCAATGCCT CAGACAATGA AAAGAGATCT TCCTTACAGT CTGCAGCTTG GCAAGGTCAT
    GTGAAAGTGG TTCAGCTCTT AATTGAACAT GGAACTTTGG TTGATAATAC ATGCAACCAG
    GGTGCTACTG CACTTTGTAT TGCAGCACAG GAAGGTCACA CTGATGTTGT TCAATTTTTG
    TTAGAAAATG GAGCAGACCC TAATCATGCA GATCAGTTTG GACGTACAGC AATGAGAGTT
    GCAGCAAAAA ATGGGCACAG TGAAATAATT AAACTATTAG AAAAATATGG TGCTTCAATA
    CCAAGTGGAT GCAACCCATC ACCTGTTCAT ACTATGGAGC AAAAAGTCTT AAATCCATCA
    TCAAAAGTAC AGTCGTCAAC AATTAAATCA AACAGTTCGT CTAGCACAGG AGGTGGTGAT
    GTGCATCCTT CTATGCGAGG TTTGTGTAAT GGGCCCGCTC ATGCCTTTAG CTCTCCATCA
    GAGTCTCCAG ATTCTACGGT GGATCGGCAC AAGTCCTCTT TATCAAATAA TTCCTTAAAA
    AGTTCTAAGA ATTCTTCTCT TCGTACAACA TCATCTACTG CAACTGCTCA AACTGTGCCT
    ATTGATAGTT TCCATTGCCT TTCATTTACA GAGCAAATTC AGCAGCATTC TCTGCCACGC
    AGTAGAAGTC GACAGTCAAT AGTTTCTCCC TCATCTACAA CACATTCCTT GGGGCAAAAT
    TACTCACCAA CTAGTGAATT TGAATGGAGT CAGGTAAAAC CCAGTTTAAA ATCAACCAAA
    ACAAATAAAA GTGGCAAAGG AGAGTCCTCA AGCAAATCAA ACTCTGTTGC AAAGAAAACA
    AAACAAAATT GCAGCTCTCA GCCTAAAGTT TTGGAATATG AAATGACTCA GTTTGACAAG
    CGAGTGCATC CTCAAGATTC CTTACCACTA AAACACATCC CTGTAGACAG TAAGATCCAA
    ATTCCATCAG TCCAACAAGA AGTTGGTCGC TCTCAACAGC AATTTCTTAT TCAACAGCAA
    AATTTAGAAC AAAAGAAAAG AAATGGCATT ATGACTAATC CCAATTATCA CCTGCAAGGA
    AATCAAGTCT TTCTTGGGAG AGTTTCTGTG CCACGTGCAG TACAAAGTCG AGGATTGCAG
    GATGTTTTAG AAGGCTATCC TTCAAGTGAG ACAGAATTAG GCCTTAAACA ATCACTGAAG
    CTACAAATTG AAGGATCTGA TCCAAGCTTT AATTATAAAA AGGAAACACC ACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951642.1
    CDS90..3686
    Translation

    Target ORF information:

    RefSeq Version XM_018096153.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096153.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCCTTC TGTTCAGAGC CTGTTGCAGC CAGGAGAATG TGAACGGAAC CCTTCAGAGG 
    CATTTAAAAG GTTTCCGAAA AATAAGTCTG GATGATCTCC GGAAAGCTTA CATAGTAAAA
    GATGTTCAAC AGTATATTCT TCATCGTTTG GACCAAGAAG AGGCATTGAG GCAACACCTA
    ACAAAAGAAA CTGCGGAAAT GTTAAATCAA CTGCACATTA AAAGCAGTGG GTGCTTTCTG
    TACCTGGAGC GTGTTTTAGA TGGAGTTGTA GAAAATTTCA TCATGCTACG AGAGATCCGT
    GATATTCCTG GAACTCTAAA TGGCTTATAC CTCTGGTTGT GTCAAAGACT TTTTGTTAGA
    AAACAATTTG CAAAGGTCCA ACCAATTCTT AATGTTATTC TGGCTGCTTG TAAGCCATTA
    ACAACTATTG AACTCTTTAA TGCAGTTTGG ACCAAAAACA TGTCATTAAC CATGGAAGAT
    TTTCAGAGGA AACTGGATGT GCTATCAAAA GTCTTGGTCG AAGGATTGGG GAATACTAAA
    ATTTTATTCC ATTATAGTTT TGCAGAGTGG CTGCTAGATG TAAAACATTG CACACAGAAA
    TATTTGTGTA ATGCAGCTGA AGGACACCGA ATGCTTGCAA TGAGCTACAC ATGTCGGGCT
    AAGGAGCTTA CGCCAATAGA AGTACAGGAG TTTGCTTTGC ATCTTATACA TTCAAATTTA
    CAGTTAACCA ATTCAGAGTT GGCTTTATGG ATGATTTTGA ATGGAACATG TGTGAAAGAC
    TCATTGTGCA CCTTAATACC AAAGGAACAA GAAGTACTAC AGTTGTTGGT AAAAGCAGGA
    GCCCATGTTG ATAGTGAGGA TGATAGAACA TGTTGCATTG TTCGACAAGC ATTGGAACGA
    GAAGATTCTA TTCGTACGTT GCTGGATAAT GGTGCTTCAG TTAACCAGTG TGATTCAAAT
    GGGAGAACTT TGCTGGCCAA CACTGCTTAT AGTGGAAATC TTGATGTGGT TAACCTTCTT
    GTTTCAAGAG GATCTGATCT TGAAATAGAA GATGCCAATG GACAGACAGC ACTTACTTTA
    GCTGCCCGCC AAGGACATGT AAAGGTTGTA AATTGTTTAA TTGGATGCGG TGCTAATATA
    AATCATTGTG ATCATGATGG ATGGACTGCA CTCAGATCTG CAGCCTGGGG TGGGCACACT
    GAAGTAGTGT CTGCACTTCT GTATGCAGGT GCAAAGGTAG ATTGTGCTGA TGCAGATAGT
    AGAACAGCTT TAAGAGCAGC AGCATGGGGT GGGCATGAAG ATATTGTGCT GAATTTACTG
    CAGCATGGAG CAGAGGTTAA TAAAGCTGAC AATGAGGGAC GAACTGCTTT AATTGCTGCA
    GCATATATGG GACATAGAGA AATTGTGGAG CATCTTTTAG GTCATGGTGC AGAGATAAAC
    CATGAGGATG TGGATGGAAG AACTGCTTTA TCAGTTGCTG CTCTTTGTGT ACCTGCAAGC
    AAAGGGCATG CATCCGTTGT TAGTCTTTTA ATTGAACAAG GTGCTGAGGT GGATCATTGT
    GATAAGGATG GAATGACTCC ACTACTTGTT GCAGCATATG AAGGCCATGT AGATGTAGTT
    GACTTACTTC TAGAGGGTGG AGCAGATGTC GATCATACTG ATAATAATGG ACGTACTCCT
    CTGCTTGCTG CTGCTTCAAT GGGGCATGCA GCAGTTGTCA ACACACTTCT GTTTTGGGGT
    GCTGCTGTTG ATAGCATTGA TAGTGAAGGT AGAACTGTGC TTAGTATAGC TTCAGCTCAA
    GGCAATGTTG AAGTTGTGCG GACACTTTTA GACCGGGGCC TTGATGAAAA TCATAGAGAT
    GATGCTGGAT GGACACCTCT GCATATGGCT GCTTTTGAAG GTCATAGATT AATCTGTGAA
    GCATTAATTG AACAAGGGGC TAGAACAAAT GAAATTGATA ATGATGGACG AATACCTCTG
    ATTCTGGCTG CACAAGAAGG GCACTATGAT TGTGTGCAAA TACTTCTAGA AAATAAATCT
    GCAGTGGATC AAAAAGGCTA TGATGGCCGA AGTGCCTTGA GAGTTGCAGC ACTGGAGGGA
    CACAGAGACA TAGTTGAATT GCTTCTCAGT AATGGAGCAG ATCTTAATGC CAAAGATGCC
    GATGGTCGAC CAACACTATA CATACTGGCC CTCGAAAATC AGCTCTCGAT GGCTGAGTAT
    TTTTTAGAAA ATGGAGCAAA TGTAGAAGCT AGCGATACTG ATGGAAGGAC AGCTCTTCAT
    GTCTCCTGCT GGCAGGGACA TTTAGAAATG GTGCAGAGTC TAATATCCTA TAAGGCTGAT
    GTCAATGCCT CAGACAATGA AAAGAGATCT TCCTTACAGT CTGCAGCTTG GCAAGGTCAT
    GTGAAAGTGG TTCAGCTCTT AATTGAACAT GGAACTTTGG TTGATAATAC ATGCAACCAG
    GGTGCTACTG CACTTTGTAT TGCAGCACAG GAAGGTCACA CTGATGTTGT TCAATTTTTG
    TTAGAAAATG GAGCAGACCC TAATCATGCA GATCAGTTTG GACGTACAGC AATGAGAGTT
    GCAGCAAAAA ATGGGCACAG TGAAATAATT AAACTATTAG AAAAATATGG TGCTTCAATA
    CCAAGTGGAT GCAACCCATC ACCTGTTCAT ACTATGGAGC AAAAAGTCTT AAATCCATCA
    TCAAAAGTAC AGTCGTCAAC AATTAAATCA AACAGTTCGT CTAGCACAGG AGGTGGTGAT
    GTGCATCCTT CTATGCGAGG TTTGTGTAAT GGGCCCGCTC ATGCCTTTAG CTCTCCATCA
    GAGTCTCCAG ATTCTACGGT GGATCGGCAC AAGTCCTCTT TATCAAATAA TTCCTTAAAA
    AGTTCTAAGA ATTCTTCTCT TCGTACAACA TCATCTACTG CAACTGCTCA AACTGTGCCT
    ATTGATAGTT TCCATTGCCT TTCATTTACA GAGCAAATTC AGCAGCATTC TCTGCCACGC
    AGTAGAAGTC GACAGTCAAT AGTTTCTCCC TCATCTACAA CACATTCCTT GGGGCAAAAT
    TACTCACCAA CTAGTGAATT TGAATGGAGT CAGGTAAAAC CCAGTTTAAA ATCAACCAAA
    ACAAATAAAA GTGGCAAAGG AGAGTCCTCA AGCAAATCAA ACTCTGTTGC AAAGAAAACA
    AAACAAAATT GCAGCTCTCA GCCTAAAGTT TTGGAATATG AAATGACTCA GTTTGACAAG
    CGAGTGCATC CTCAAGATTC CTTACCACTA AAACACATCC CTGTAGACAG TAAGATCCAA
    ATTCCATCAG TCCAACAAGA AGTTGGTCGC TCTCAACAGC AATTTCTTAT TCAACAGCAA
    AATTTAGAAC AAAAGAAAAG AAATGGCATT ATGACTAATC CCAATTATCA CCTGCAAGGA
    AATCAAGTCT TTCTTGGGAG AGTTTCTGTG CCACGTGCAG TACAAAGTCG AGGATTGCAG
    GATGTTTTAG AAGGCTATCC TTCAAGTGAG ACAGAATTAG GCCTTAAACA ATCACTGAAG
    CTACAAATTG AAGGATCTGA TCCAAGCTTT AATTATAAAA AGGAAACACC ACTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48105
    Clone ID Related Accession (Same CDS sequence) XM_002941998.5 , XM_031902563.1 , XM_018096150.2
    Accession Version XM_002941998.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941998.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942044.1
    CDS466..4698
    Translation

    Target ORF information:

    RefSeq Version XM_002941998.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941998.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48105
    Clone ID Related Accession (Same CDS sequence) XM_002941998.5 , XM_031902563.1 , XM_018096150.2
    Accession Version XM_031902563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758423.1
    CDS751..4983
    Translation

    Target ORF information:

    RefSeq Version XM_031902563.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48105
    Clone ID Related Accession (Same CDS sequence) XM_002941998.5 , XM_031902563.1 , XM_018096150.2
    Accession Version XM_018096150.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096150.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951639.1
    CDS638..4870
    Translation

    Target ORF information:

    RefSeq Version XM_018096150.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096150.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TGGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTTGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTACAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04555
    Clone ID Related Accession (Same CDS sequence) XM_002941998.4 , XM_018096150.1
    Accession Version XM_002941998.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941998.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TAGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTCGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTGCAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942044.1
    CDS465..4697
    Translation

    Target ORF information:

    RefSeq Version XM_002941998.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002941998.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TAGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTCGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTGCAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04555
    Clone ID Related Accession (Same CDS sequence) XM_002941998.4 , XM_018096150.1
    Accession Version XM_018096150.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TAGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTCGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTGCAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951639.1
    CDS461..4693
    Translation

    Target ORF information:

    RefSeq Version XM_018096150.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ankyrin repeat domain 50 (ankrd50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096150.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTCAGA CCAGTTTACT GCAAGGAAAA CCTTTCTACT GCAGGGAATG GATTTTCCAT 
    AAACTTCAGC ACTGCCTGCA GGAAAAACTA AACTGCAGCA GTAGCACCAC AAACAAACAT
    TCACTTGTGG CTAATTCTGG GAGCAATGGT GGAAGCACAG GAAAAGGCAC AGCATGGGGT
    TTGTTATTTG TTGGGGGCCC TGGAAGTGGA AAGACAGCAC TTTGTACAGA GCTGCTTTGG
    CCAAGTTCAC CTGCTGGCTT GCAGAGGGGA GTACATCGTC ATGCTCTAGC ATATCATTTT
    TGCAGAGCGC AGGACTCGGA CACAATGTGC GTTGGAAGCT TTATTAGAAG TTTGGTTGAT
    CAGATCTGTC AAGGTGGACT AATGCCAGGT TTTGAAGACA AGTTAAATGA CCCTTCTGTT
    CAGAGCCTGT TGCAGCCAGG AGAATGTGAA CGGAACCCTT CAGAGGCATT TAAAAGGTGT
    GTTCTTCTAC CTCTTTTGGC CATAAAAGCC CCTGCCCAGA GTCTTTTCAT GGTTGTTGAC
    TCTGTTGATG AAGGCTGTAA CATTGTTGAA GGAGAACACA GACCCTCAGG TTTATCTGGA
    ACAATAGCAG ATCTTTTAGC AACTCACCAT GAGTTTTTTC CCCCATGGCT ACTACTCATC
    TGTTCAGCAC GCAAGCAAAG TAGAACAGTG ACAAAAATGT TTACAGGTTT CCGAAAAATA
    AGTCTGGATG ATCTCCGGAA AGCTTACATA GTAAAAGATG TTCAACAGTA TATTCTTCAT
    CGTTTGGACC AAGAAGAGGC ATTGAGGCAA CACCTAACAA AAGAAACTGC GGAAATGTTA
    AATCAACTGC ACATTAAAAG CAGTGGGTGC TTTCTGTACC TGGAGCGTGT TTTAGATGGA
    GTTGTAGAAA ATTTCATCAT GCTACGAGAG ATCCGTGATA TTCCTGGAAC TCTAAATGGC
    TTATACCTCT GGTTGTGTCA AAGACTTTTT GTTAGAAAAC AATTTGCAAA GGTCCAACCA
    ATTCTTAATG TTATTCTGGC TGCTTGTAAG CCATTAACAA CTATTGAACT CTTTAATGCA
    GTTTGGACCA AAAACATGTC ATTAACCATG GAAGATTTTC AGAGGAAACT GGATGTGCTA
    TCAAAAGTCT TGGTCGAAGG ATTGGGGAAT ACTAAAATTT TATTCCATTA TAGTTTTGCA
    GAGTGGCTGC TAGATGTAAA ACATTGCACA CAGAAATATT TGTGTAATGC AGCTGAAGGA
    CACCGAATGC TTGCAATGAG CTACACATGT CGGGCTAAGG AGCTTACGCC AATAGAAGTA
    CAGGAGTTTG CTTTGCATCT TATACATTCA AATTTACAGT TAACCAATTC AGAGTTGGCT
    TTATGGATGA TTTTGAATGG AACATGTGTG AAAGACTCAT TGTGCACCTT AATACCAAAG
    GAACAAGAAG TACTACAGTT GTTGGTAAAA GCAGGAGCCC ATGTTGATAG TGAGGATGAT
    AGAACATGTT GCATTGTTCG ACAAGCATTG GAACGAGAAG ATTCTATTCG TACGTTGCTG
    GATAATGGTG CTTCAGTTAA CCAGTGTGAT TCAAATGGGA GAACTTTGCT GGCCAACACT
    GCTTATAGTG GAAATCTTGA TGTGGTTAAC CTTCTTGTTT CAAGAGGATC TGATCTTGAA
    ATAGAAGATG CCAATGGACA GACAGCACTT ACTTTAGCTG CCCGCCAAGG ACATGTAAAG
    GTTGTAAATT GTTTAATTGG ATGCGGTGCT AATATAAATC ATTGTGATCA TGATGGATGG
    ACTGCACTCA GATCTGCAGC CTGGGGTGGG CACACTGAAG TAGTGTCTGC ACTTCTGTAT
    GCAGGTGCAA AGGTAGATTG TGCTGATGCA GATAGTAGAA CAGCTTTAAG AGCAGCAGCA
    TGGGGTGGGC ATGAAGATAT TGTGCTGAAT TTACTGCAGC ATGGAGCAGA GGTTAATAAA
    GCTGACAATG AGGGACGAAC TGCTTTAATT GCTGCAGCAT ATATGGGACA TAGAGAAATT
    GTGGAGCATC TTTTAGGTCA TGGTGCAGAG ATAAACCATG AGGATGTGGA TGGAAGAACT
    GCTTTATCAG TTGCTGCTCT TTGTGTACCT GCAAGCAAAG GGCATGCATC CGTTGTTAGT
    CTTTTAATTG AACAAGGTGC TGAGGTGGAT CATTGTGATA AGGATGGAAT GACTCCACTA
    CTTGTTGCAG CATATGAAGG CCATGTAGAT GTAGTTGACT TACTTCTAGA GGGTGGAGCA
    GATGTCGATC ATACTGATAA TAATGGACGT ACTCCTCTGC TTGCTGCTGC TTCAATGGGG
    CATGCAGCAG TTGTCAACAC ACTTCTGTTT TGGGGTGCTG CTGTTGATAG CATTGATAGT
    GAAGGTAGAA CTGTGCTTAG TATAGCTTCA GCTCAAGGCA ATGTTGAAGT TGTGCGGACA
    CTTTTAGACC GGGGCCTTGA TGAAAATCAT AGAGATGATG CTGGATGGAC ACCTCTGCAT
    ATGGCTGCTT TTGAAGGTCA TAGATTAATC TGTGAAGCAT TAATTGAACA AGGGGCTAGA
    ACAAATGAAA TTGATAATGA TGGACGAATA CCTCTGATTC TGGCTGCACA AGAAGGGCAC
    TATGATTGTG TGCAAATACT TCTAGAAAAT AAATCTGCAG TGGATCAAAA AGGCTATGAT
    GGCCGAAGTG CCTTGAGAGT TGCAGCACTG GAGGGACACA GAGACATAGT TGAATTGCTT
    CTCAGTAATG GAGCAGATCT TAATGCCAAA GATGCCGATG GTCGACCAAC ACTATACATA
    CTGGCCCTCG AAAATCAGCT CTCGATGGCT GAGTATTTTT TAGAAAATGG AGCAAATGTA
    GAAGCTAGCG ATACTGATGG AAGGACAGCT CTTCATGTCT CCTGCTGGCA GGGACATTTA
    GAAATGGTGC AGAGTCTAAT ATCCTATAAG GCTGATGTCA ATGCCTCAGA CAATGAAAAG
    AGATCTTCCT TACAGTCTGC AGCTTGGCAA GGTCATGTGA AAGTGGTTCA GCTCTTAATT
    GAACATGGAA CTTTGGTTGA TAATACATGC AACCAGGGTG CTACTGCACT TTGTATTGCA
    GCACAGGAAG GTCACACTGA TGTTGTTCAA TTTTTGTTAG AAAATGGAGC AGACCCTAAT
    CATGCAGATC AGTTTGGACG TACAGCAATG AGAGTTGCAG CAAAAAATGG GCACAGTGAA
    ATAATTAAAC TATTAGAAAA ATATGGTGCT TCAATACCAA GTGGATGCAA CCCATCACCT
    GTTCATACTA TGGAGCAAAA AGTCTTAAAT CCATCATCAA AAGTACAGTC GTCAACAATT
    AAATCAAACA GTTCGTCTAG CACAGGAGGT GGTGATGTGC ATCCTTCTAT GCGAGGTTTG
    TGTAATGGGC CCGCTCATGC CTTTAGCTCT CCATCAGAGT CTCCAGATTC TACGGTGGAT
    CGGCACAAGT CCTCTTTATC AAATAATTCC TTAAAAAGTT CTAAGAATTC TTCTCTTCGT
    ACAACATCAT CTACTGCAAC TGCTCAAACT GTGCCTATTG ATAGTTTCCA TTGCCTTTCA
    TTTACAGAGC AAATTCAGCA GCATTCTCTG CCACGCAGTA GAAGTCGACA GTCAATAGTT
    TCTCCCTCAT CTACAACACA TTCCTTGGGG CAAAATTACT CACCAACTAG TGAATTTGAA
    TGGAGTCAGG TAAAACCCAG TTTAAAATCA ACCAAAACAA ATAAAAGTGG CAAAGGAGAG
    TCCTCAAGCA AATCAAACTC TGTTGCAAAG AAAACAAAAC AAAATTGCAG CTCTCAGCCT
    AAAGTTTTGG AATATGAAAT GACTCAGTTT GACAAGCGAG TGCATCCTCA AGATTCCTTA
    CCACTAAAAC ACATCCCTGT AGACAGTAAG ATCCAAATTC CATCAGTCCA ACAAGAAGTT
    GGTCGCTCTC AACAGCAATT TCTTATTCAA CAGCAAAATT TAGAACAAAA GAAAAGAAAT
    GGCATTATGA CTAATCCCAA TTATCACCTG CAAGGAAATC AAGTCTTTCT TGGGAGAGTT
    TCTGTGCCAC GTGCAGTACA AAGTCGAGGA TTGCAGGATG TTTTAGAAGG CTATCCTTCA
    AGTGAGACAG AATTAGGCCT TAAACAATCA CTGAAGCTAC AAATTGAAGG ATCTGATCCA
    AGCTTTAATT ATAAAAAGGA AACACCACTA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.