ANKRD50 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD50
Entrez Gene ID	57182
Full Name	ankyrin repeat domain 50
General protein information	Preferred Names ankyrin repeat domain 50 Names ankyrin repeat domain-containing protein 50
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q28.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001167882.1	NP_001161354.1	ankyrin repeat domain-containing protein 50 isoform 2
NM_001167882.2	NP_001161354.1	ankyrin repeat domain-containing protein 50 isoform 2
XM_017008471.1	XP_016863960.1	ankyrin repeat domain-containing protein 50 isoform X1
NM_020337.2	NP_065070.1	ankyrin repeat domain-containing protein 50 isoform 1
NM_020337.3	NP_065070.1	ankyrin repeat domain-containing protein 50 isoform 1

Pathways from BioSystems

Homologies

	ANKRD50	XP_001104758.1
Gallus gallus (chicken)	ANKRD50	XP_420618.4
Canis lupus familiaris (dog)	ANKRD50	XP_540955.2
Danio rerio (zebrafish)	si:dkey-145p14.5	XP_696361.3
Pan troglodytes (chimpanzee)	ANKRD50	XP_517429.1
Homo sapiens (human)	ANKRD50	NP_065070.1
Bos taurus (cattle)	ANKRD50	NP_001192878.1
Rattus norvegicus (Norway rat)	Ankrd50	NP_001178535.1
Xenopus tropicalis (tropical clawed frog)	ankrd50	XP_002942044.1

Gene Ontology
Bibliography

Related articles in PubMed

Retromer- and WASH-dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.
Kvainickas A, Orgaz AJ, N?gele H, Diedrich B, Heesom KJ, Dengjel J, Cullen PJ, Steinberg F
Journal of cell science130(2)382-395(2017 01)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.
McGough IJ, Steinberg F, Gallon M, Yatsu A, Ohbayashi N, Heesom KJ, Fukuda M, Cullen PJ
Journal of cell science127(Pt 22)4940-53(2014 Nov)

A physical interaction network of dengue virus and human proteins.
Khadka S, Vangeloff AD, Zhang C, Siddavatam P, Heaton NS, Wang L, Sengupta R, Sahasrabudhe S, Randall G, Gribskov M, Kuhn RJ, Perera R, LaCount DJ
Molecular & cellular proteomics : MCP10(12)M111.012187(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ANKRD50 simultaneously engages multiple parts of the SNX27-retromer-WASH complex machinery in a direct and co-operative interaction network that is needed to efficiently recycle the nutrient transporters.
Title: Retromer- and WASH-dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.

The following ANKRD50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04091	NM_001167882.2 Latest version!	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu03351	NM_020337.3 Latest version!	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu03351	XM_017008471.1 Latest version!	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu04091	NM_001167882.1	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu03351	NM_020337.2	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD50 ( NM_001167882.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161354.1 Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04091
Clone ID Related Accession (Same CDS sequence)	NM_001167882.1 , NM_001167882.2
Accession Version	NM_001167882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 50 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295135.1, BC024725.1 and AB033049.2. Transcript Variant: This variant (2) lacks an internal exon in the 5' region, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK295135.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144, SAMN01820693 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCTC CCCAGCAAAG CCTATACCTG CTTGTTGATT CTGTTGATGA AGGGTGTAAC 
ATTACTGAAG GTGAACAAAC GTCTACCAGC TTATCTGGGA CTGTTGCAGC ACTTTTAGCT 
GGTCACCATG AGTTCTTTCC ACCATGGCTA TTGCTTCTCT GTTCTGCCCG AAAGCAGAGT 
AAGGCTGTTA CTAAAATGTT TACTGGTTTT CGAAAAATAA GTTTAGATGA CCTTCGGAAG 
GCATATATCG TCAAGGATGT TCAGCAGTAC ATTCTTCATC GTTTAGATCA AGAAGAAGCT 
TTGCGACAAC ACCTCACAAA AGAAACTGCA GAGATGTTAA ATCAACTGCA CATTAAAAGC 
AGTGGATGCT TTCTTTACCT AGAACGAGTT TTAGATGGAG TTGTAGAAAA TTTTATTATG 
TTAAGAGAAA TTCGTGACAT CCCAGGAACT CTAAATGGTT TATATCTCTG GCTGTGCCAA 
AGACTTTTTG TAAGAAAACA ATTTGCAAAG GTTCAGCCTA TTTTGAATGT GATTCTTGCA 
GCCTGCCGAC CTTTGACCAT AACGGAATTA TATCACGCAG TATGGACCAA AAACATGTCG 
TTAACTTTGG AAGATTTTCA ACGCAAGTTA GATATCCTCT CCAAACTTCT TGTTGATGGA 
CTAGGAAATA CAAAAATACT GTTTCATTAT AGTTTTGCCG AGTGGCTTCT GGATGTGAAA 
CACTGTACTC AGAAGTATTT ATGTAATGCA GCAGAAGGAC ACAGAATGTT GGCTATGAGT 
TATACCTGTC AAGCCAAGAA TTTAACACCA TTGGAAGCAC AAGAATTTGC ATTGCACTTA 
ATTAACTCAA ACTTACAATT AGAGACAGCG GAGTTAGCTC TGTGGATGAT ATGGAATGGT 
ACACCTGTCA GAGATTCCCT TTCTACTTTG ATACCCAAGG AACAAGAAGT GCTACAGCTG 
TTGGTTAAAG CTGGGGCTCA TGTCAACAGT GAAGACGATC GCACATCATG CATAGTTCGA 
CAAGCCTTAG AAAGAGAGGA TTCCATTCGG ACATTATTAG ATAATGGAGC TTCAGTAAAT 
CAGTGTGATT CAAATGGGAG AACATTATTG GCTAATGCTG CATATAGTGG CAGTCTTGAT 
GTAGTCAATT TACTTGTCTC TAGGGGAGCA GATTTAGAGA TAGAAGATGC TCATGGACAT 
ACACCACTCA CTCTAGCGGC TAGACAGGGA CATACCAAGG TGGTTAATTG TTTGATTGGG 
TGTGGAGCAA ATATTAATCA TACTGATCAA GATGGTTGGA CAGCATTAAG ATCTGCTGCT 
TGGGGTGGCC ATACTGAGGT AGTTTCTGCA CTACTTTATG CTGGCGTAAA AGTGGATTGT 
GCAGATGCTG ATAGCCGAAC AGCTTTGAGA GCAGCAGCAT GGGGAGGACA CGAGGATATT 
GTACTGAATT TGCTACAACA TGGCGCTGAA GTGAACAAAG CTGATAATGA AGGTAGAACT 
GCTTTGATAG CAGCAGCATA CATGGGACAT AGAGAGATTG TGGAACACCT ACTGGACCAT 
GGAGCAGAAG TAAATCATGA GGATGTTGAT GGCAGGACTG CACTCTCTGT AGCTGCACTT 
TGTGTGCCTG CAAGTAAAGG GCACGCATCA GTTGTTAGCC TTTTAATTGA TCGAGGTGCT 
GAAGTAGATC ATTGTGATAA AGATGGCATG ACTCCACTGC TGGTAGCTGC CTATGAAGGA 
CATGTTGATG TGGTTGACTT GCTTCTAGAA GGGGGAGCAG ATGTAGATCA CACAGATAAC 
AATGGCCGTA CACCCCTCTT AGCAGCAGCG TCTATGGGTC ATGCATCAGT TGTAAATACA 
CTTTTGTTTT GGGGTGCAGC TGTGGATAGT ATTGATAGTG AAGGTAGGAC AGTCCTCAGT 
ATAGCTTCAG CACAAGGAAA TGTTGAGGTG GTACGTACTC TACTGGATAG AGGGTTAGAT 
GAAAATCACA GAGATGATGC TGGATGGACA CCTTTGCACA TGGCAGCTTT TGAAGGGCAC 
AGATTGATAT GTGAAGCACT TATTGAACAA GGTGCTAGAA CAAATGAGAT TGACAATGAT 
GGACGAATCC CTTTCATATT AGCTTCACAA GAGGGTCATT ATGATTGTGT TCAAATATTA 
CTGGAAAACA AATCCAACAT TGATCAAAGA GGTTATGATG GAAGAAATGC ACTGCGGGTT 
GCTGCATTAG AAGGGCACAG GGACATTGTT GAATTGCTTT TTAGCCATGG TGCTGATGTT 
AACTGCAAAG ATGCTGATGG TCGGCCTACA CTTTATATCT TGGCCTTAGA AAATCAGCTT 
ACAATGGCCG AATATTTTTT AGAAAATGGT GCAAACGTAG AAGCAAGTGA TGCTGAAGGA 
AGGACAGCAC TTCATGTGTC TTGTTGGCAA GGCCATATGG AAATGGTGCA GGTCCTGATA 
GCATACCATG CTGACGTCAA TGCTGCAGAC AATGAAAAGC GCTCTGCTTT GCAGTCTGCA 
GCCTGGCAGG GCCATGTAAA AGTGGTTCAG CTTCTGATTG AGCATGGTGC TGTAGTTGAC 
CATACATGTA ACCAAGGTGC AACTGCACTC TGTATTGCAG CCCAGGAAGG GCACATTGAT 
GTTGTTCAGG TCTTATTAGA GCATGGTGCT GATCCAAACC ATGCTGATCA ATTTGGACGC 
ACTGCTATGC GTGTTGCAGC CAAAAATGGA CATTCTCAGA TAATTAAATT ATTAGAAAAA 
TATGGTGCAT CTAGTTTGAA TGGCTGTTCC CCATCTCCTG TTCACACAAT GGAGCAAAAA 
CCTCTACAGT CATTGTCTTC AAAAGTGCAG TCATTAACAA TTAAATCAAA TAGCTCTGGT 
AGTACTGGTG GAGGGGATAT GCAGCCTTCG TTACGTGGTT TACCTAATGG GCCTACTCAT 
GCTTTTAGTT CTCCTTCAGA ATCTCCAGAT TCTACAGTTG ACCGGCAGAA GTCATCACTG 
TCAAATAATT CCCTGAAAAG CTCAAAAAAT TCATCTTTGA GAACTACTTC ATCTACAGCA 
ACGGCTCAAA CAGTGCCAAT TGATAGCTTT CATAACTTGT CATTTACAGA ACAAATTCAG 
CAGCATTCAT TGCCACGCAG TAGAAGTCGA CAGTCAATTG TTTCCCCATC TTCCACAACA 
CAGTCCTTAG GACAGAGTCA TAATTCACCA AGTAGTGAAT TTGAGTGGAG TCAAGTAAAG 
CCCAGTTTGA AGTCAACTAA AGCAAGTAAA GGGGGGAAAT CAGAAAATTC TGCCAAGTCT 
GGATCAGCTG GGAAAAAAGC GAAACAAAGT AATTCTTCAC AGCCAAAGGT TTTAGAATAT 
GAAATGACTC AGTTTGATAG AAGAGGACCT ATAGCCAAAT CCGGGACTGC TGCACCACCT 
AAACAAATGC CAGCAGAATC TCAATGCAAA ATTATGATAC CTTCAGCTCA GCAGGAAATT 
GGTCGATCTC AACAGCAGTT TCTTATTCAC CAACAAAGTG GGGAACAGAA GAAGAGAAAT 
GGAATAATGA CAAATCCAAA TTATCATCTT CAGAGCAACC AGGTTTTTCT TGGTAGGGTT 
TCAGTCCCAC GAACAATGCA AGATAGAGGG CATCAGGAAG TGTTGGAGGG ATACCCTTCC 
TCAGAGACAG AATTAAGCCT TAAACAAGCT CTGAAGCTTC AGATTGAAGG TTCTGACCCT 
AGCTTCAACT ATAAAAAGGA AACACCATTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161354.1
CDS	301..4053
Translation	MKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_001167882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167882.1

ORF Insert Sequence:

ATGAAGCCTC CCCAGCAAAG CCTATACCTG CTTGTTGATT CTGTTGATGA AGGGTGTAAC 
ATTACTGAAG GTGAACAAAC GTCTACCAGC TTATCTGGGA CTGTTGCAGC ACTTTTAGCT 
GGTCACCATG AGTTCTTTCC ACCATGGCTA TTGCTTCTCT GTTCTGCCCG AAAGCAGAGT 
AAGGCTGTTA CTAAAATGTT TACTGGTTTT CGAAAAATAA GTTTAGATGA CCTTCGGAAG 
GCATATATCG TCAAGGATGT TCAGCAGTAC ATTCTTCATC GTTTAGATCA AGAAGAAGCT 
TTGCGACAAC ACCTCACAAA AGAAACTGCA GAGATGTTAA ATCAACTGCA CATTAAAAGC 
AGTGGATGCT TTCTTTACCT AGAACGAGTT TTAGATGGAG TTGTAGAAAA TTTTATTATG 
TTAAGAGAAA TTCGTGACAT CCCAGGAACT CTAAATGGTT TATATCTCTG GCTGTGCCAA 
AGACTTTTTG TAAGAAAACA ATTTGCAAAG GTTCAGCCTA TTTTGAATGT GATTCTTGCA 
GCCTGCCGAC CTTTGACCAT AACGGAATTA TATCACGCAG TATGGACCAA AAACATGTCG 
TTAACTTTGG AAGATTTTCA ACGCAAGTTA GATATCCTCT CCAAACTTCT TGTTGATGGA 
CTAGGAAATA CAAAAATACT GTTTCATTAT AGTTTTGCCG AGTGGCTTCT GGATGTGAAA 
CACTGTACTC AGAAGTATTT ATGTAATGCA GCAGAAGGAC ACAGAATGTT GGCTATGAGT 
TATACCTGTC AAGCCAAGAA TTTAACACCA TTGGAAGCAC AAGAATTTGC ATTGCACTTA 
ATTAACTCAA ACTTACAATT AGAGACAGCG GAGTTAGCTC TGTGGATGAT ATGGAATGGT 
ACACCTGTCA GAGATTCCCT TTCTACTTTG ATACCCAAGG AACAAGAAGT GCTACAGCTG 
TTGGTTAAAG CTGGGGCTCA TGTCAACAGT GAAGACGATC GCACATCATG CATAGTTCGA 
CAAGCCTTAG AAAGAGAGGA TTCCATTCGG ACATTATTAG ATAATGGAGC TTCAGTAAAT 
CAGTGTGATT CAAATGGGAG AACATTATTG GCTAATGCTG CATATAGTGG CAGTCTTGAT 
GTAGTCAATT TACTTGTCTC TAGGGGAGCA GATTTAGAGA TAGAAGATGC TCATGGACAT 
ACACCACTCA CTCTAGCGGC TAGACAGGGA CATACCAAGG TGGTTAATTG TTTGATTGGG 
TGTGGAGCAA ATATTAATCA TACTGATCAA GATGGTTGGA CAGCATTAAG ATCTGCTGCT 
TGGGGTGGCC ATACTGAGGT AGTTTCTGCA CTACTTTATG CTGGCGTAAA AGTGGATTGT 
GCAGATGCTG ATAGCCGAAC AGCTTTGAGA GCAGCAGCAT GGGGAGGACA CGAGGATATT 
GTACTGAATT TGCTACAACA TGGCGCTGAA GTGAACAAAG CTGATAATGA AGGTAGAACT 
GCTTTGATAG CAGCAGCATA CATGGGACAT AGAGAGATTG TGGAACACCT ACTGGACCAT 
GGAGCAGAAG TAAATCATGA GGATGTTGAT GGCAGGACTG CACTCTCTGT AGCTGCACTT 
TGTGTGCCTG CAAGTAAAGG GCACGCATCA GTTGTTAGCC TTTTAATTGA TCGAGGTGCT 
GAAGTAGATC ATTGTGATAA AGATGGCATG ACTCCACTGC TGGTAGCTGC CTATGAAGGA 
CATGTTGATG TGGTTGACTT GCTTCTAGAA GGGGGAGCAG ATGTAGATCA CACAGATAAC 
AATGGCCGTA CACCCCTCTT AGCAGCAGCG TCTATGGGTC ATGCATCAGT TGTAAATACA 
CTTTTGTTTT GGGGTGCAGC TGTGGATAGT ATTGATAGTG AAGGTAGGAC AGTCCTCAGT 
ATAGCTTCAG CACAAGGAAA TGTTGAGGTG GTACGTACTC TACTGGATAG AGGGTTAGAT 
GAAAATCACA GAGATGATGC TGGATGGACA CCTTTGCACA TGGCAGCTTT TGAAGGGCAC 
AGATTGATAT GTGAAGCACT TATTGAACAA GGTGCTAGAA CAAATGAGAT TGACAATGAT 
GGACGAATCC CTTTCATATT AGCTTCACAA GAGGGTCATT ATGATTGTGT TCAAATATTA 
CTGGAAAACA AATCCAACAT TGATCAAAGA GGTTATGATG GAAGAAATGC ACTGCGGGTT 
GCTGCATTAG AAGGGCACAG GGACATTGTT GAATTGCTTT TTAGCCATGG TGCTGATGTT 
AACTGCAAAG ATGCTGATGG TCGGCCTACA CTTTATATCT TGGCCTTAGA AAATCAGCTT 
ACAATGGCCG AATATTTTTT AGAAAATGGT GCAAACGTAG AAGCAAGTGA TGCTGAAGGA 
AGGACAGCAC TTCATGTGTC TTGTTGGCAA GGCCATATGG AAATGGTGCA GGTCCTGATA 
GCATACCATG CTGACGTCAA TGCTGCAGAC AATGAAAAGC GCTCTGCTTT GCAGTCTGCA 
GCCTGGCAGG GCCATGTAAA AGTGGTTCAG CTTCTGATTG AGCATGGTGC TGTAGTTGAC 
CATACATGTA ACCAAGGTGC AACTGCACTC TGTATTGCAG CCCAGGAAGG GCACATTGAT 
GTTGTTCAGG TCTTATTAGA GCATGGTGCT GATCCAAACC ATGCTGATCA ATTTGGACGC 
ACTGCTATGC GTGTTGCAGC CAAAAATGGA CATTCTCAGA TAATTAAATT ATTAGAAAAA 
TATGGTGCAT CTAGTTTGAA TGGCTGTTCC CCATCTCCTG TTCACACAAT GGAGCAAAAA 
CCTCTACAGT CATTGTCTTC AAAAGTGCAG TCATTAACAA TTAAATCAAA TAGCTCTGGT 
AGTACTGGTG GAGGGGATAT GCAGCCTTCG TTACGTGGTT TACCTAATGG GCCTACTCAT 
GCTTTTAGTT CTCCTTCAGA ATCTCCAGAT TCTACAGTTG ACCGGCAGAA GTCATCACTG 
TCAAATAATT CCCTGAAAAG CTCAAAAAAT TCATCTTTGA GAACTACTTC ATCTACAGCA 
ACGGCTCAAA CAGTGCCAAT TGATAGCTTT CATAACTTGT CATTTACAGA ACAAATTCAG 
CAGCATTCAT TGCCACGCAG TAGAAGTCGA CAGTCAATTG TTTCCCCATC TTCCACAACA 
CAGTCCTTAG GACAGAGTCA TAATTCACCA AGTAGTGAAT TTGAGTGGAG TCAAGTAAAG 
CCCAGTTTGA AGTCAACTAA AGCAAGTAAA GGGGGGAAAT CAGAAAATTC TGCCAAGTCT 
GGATCAGCTG GGAAAAAAGC GAAACAAAGT AATTCTTCAC AGCCAAAGGT TTTAGAATAT 
GAAATGACTC AGTTTGATAG AAGAGGACCT ATAGCCAAAT CCGGGACTGC TGCACCACCT 
AAACAAATGC CAGCAGAATC TCAATGCAAA ATTATGATAC CTTCAGCTCA GCAGGAAATT 
GGTCGATCTC AACAGCAGTT TCTTATTCAC CAACAAAGTG GGGAACAGAA GAAGAGAAAT 
GGAATAATGA CAAATCCAAA TTATCATCTT CAGAGCAACC AGGTTTTTCT TGGTAGGGTT 
TCAGTCCCAC GAACAATGCA AGATAGAGGG CATCAGGAAG TGTTGGAGGG ATACCCTTCC 
TCAGAGACAG AATTAAGCCT TAAACAAGCT CTGAAGCTTC AGATTGAAGG TTCTGACCCT 
AGCTTCAACT ATAAAAAGGA AACACCATTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( NM_001167882.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001161354.1 Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04091
Clone ID Related Accession (Same CDS sequence)	NM_001167882.1 , NM_001167882.2
Accession Version	NM_001167882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 50 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295135.1, BC024725.1, AB033049.2 and AC105421.2. On May 31, 2019 this sequence version replaced NM_001167882.1. Transcript Variant: This variant (2) lacks an internal exon in the 5' region, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK295135.1, DRR138512.82939.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144, SAMN01820693 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCTC CCCAGCAAAG CCTATACCTG CTTGTTGATT CTGTTGATGA AGGGTGTAAC 
ATTACTGAAG GTGAACAAAC GTCTACCAGC TTATCTGGGA CTGTTGCAGC ACTTTTAGCT 
GGTCACCATG AGTTCTTTCC ACCATGGCTA TTGCTTCTCT GTTCTGCCCG AAAGCAGAGT 
AAGGCTGTTA CTAAAATGTT TACTGGTTTT CGAAAAATAA GTTTAGATGA CCTTCGGAAG 
GCATATATCG TCAAGGATGT TCAGCAGTAC ATTCTTCATC GTTTAGATCA AGAAGAAGCT 
TTGCGACAAC ACCTCACAAA AGAAACTGCA GAGATGTTAA ATCAACTGCA CATTAAAAGC 
AGTGGATGCT TTCTTTACCT AGAACGAGTT TTAGATGGAG TTGTAGAAAA TTTTATTATG 
TTAAGAGAAA TTCGTGACAT CCCAGGAACT CTAAATGGTT TATATCTCTG GCTGTGCCAA 
AGACTTTTTG TAAGAAAACA ATTTGCAAAG GTTCAGCCTA TTTTGAATGT GATTCTTGCA 
GCCTGCCGAC CTTTGACCAT AACGGAATTA TATCACGCAG TATGGACCAA AAACATGTCG 
TTAACTTTGG AAGATTTTCA ACGCAAGTTA GATATCCTCT CCAAACTTCT TGTTGATGGA 
CTAGGAAATA CAAAAATACT GTTTCATTAT AGTTTTGCCG AGTGGCTTCT GGATGTGAAA 
CACTGTACTC AGAAGTATTT ATGTAATGCA GCAGAAGGAC ACAGAATGTT GGCTATGAGT 
TATACCTGTC AAGCCAAGAA TTTAACACCA TTGGAAGCAC AAGAATTTGC ATTGCACTTA 
ATTAACTCAA ACTTACAATT AGAGACAGCG GAGTTAGCTC TGTGGATGAT ATGGAATGGT 
ACACCTGTCA GAGATTCCCT TTCTACTTTG ATACCCAAGG AACAAGAAGT GCTACAGCTG 
TTGGTTAAAG CTGGGGCTCA TGTCAACAGT GAAGACGATC GCACATCATG CATAGTTCGA 
CAAGCCTTAG AAAGAGAGGA TTCCATTCGG ACATTATTAG ATAATGGAGC TTCAGTAAAT 
CAGTGTGATT CAAATGGGAG AACATTATTG GCTAATGCTG CATATAGTGG CAGTCTTGAT 
GTAGTCAATT TACTTGTCTC TAGGGGAGCA GATTTAGAGA TAGAAGATGC TCATGGACAT 
ACACCACTCA CTCTAGCGGC TAGACAGGGA CATACCAAGG TGGTTAATTG TTTGATTGGG 
TGTGGAGCAA ATATTAATCA TACTGATCAA GATGGTTGGA CAGCATTAAG ATCTGCTGCT 
TGGGGTGGCC ATACTGAGGT AGTTTCTGCA CTACTTTATG CTGGCGTAAA AGTGGATTGT 
GCAGATGCTG ATAGCCGAAC AGCTTTGAGA GCAGCAGCAT GGGGAGGACA CGAGGATATT 
GTACTGAATT TGCTACAACA TGGCGCTGAA GTGAACAAAG CTGATAATGA AGGTAGAACT 
GCTTTGATAG CAGCAGCATA CATGGGACAT AGAGAGATTG TGGAACACCT ACTGGACCAT 
GGAGCAGAAG TAAATCATGA GGATGTTGAT GGCAGGACTG CACTCTCTGT AGCTGCACTT 
TGTGTGCCTG CAAGTAAAGG GCACGCATCA GTTGTTAGCC TTTTAATTGA TCGAGGTGCT 
GAAGTAGATC ATTGTGATAA AGATGGCATG ACTCCACTGC TGGTAGCTGC CTATGAAGGA 
CATGTTGATG TGGTTGACTT GCTTCTAGAA GGGGGAGCAG ATGTAGATCA CACAGATAAC 
AATGGCCGTA CACCCCTCTT AGCAGCAGCG TCTATGGGTC ATGCATCAGT TGTAAATACA 
CTTTTGTTTT GGGGTGCAGC TGTGGATAGT ATTGATAGTG AAGGTAGGAC AGTCCTCAGT 
ATAGCTTCAG CACAAGGAAA TGTTGAGGTG GTACGTACTC TACTGGATAG AGGGTTAGAT 
GAAAATCACA GAGATGATGC TGGATGGACA CCTTTGCACA TGGCAGCTTT TGAAGGGCAC 
AGATTGATAT GTGAAGCACT TATTGAACAA GGTGCTAGAA CAAATGAGAT TGACAATGAT 
GGACGAATCC CTTTCATATT AGCTTCACAA GAGGGTCATT ATGATTGTGT TCAAATATTA 
CTGGAAAACA AATCCAACAT TGATCAAAGA GGTTATGATG GAAGAAATGC ACTGCGGGTT 
GCTGCATTAG AAGGGCACAG GGACATTGTT GAATTGCTTT TTAGCCATGG TGCTGATGTT 
AACTGCAAAG ATGCTGATGG TCGGCCTACA CTTTATATCT TGGCCTTAGA AAATCAGCTT 
ACAATGGCCG AATATTTTTT AGAAAATGGT GCAAACGTAG AAGCAAGTGA TGCTGAAGGA 
AGGACAGCAC TTCATGTGTC TTGTTGGCAA GGCCATATGG AAATGGTGCA GGTCCTGATA 
GCATACCATG CTGACGTCAA TGCTGCAGAC AATGAAAAGC GCTCTGCTTT GCAGTCTGCA 
GCCTGGCAGG GCCATGTAAA AGTGGTTCAG CTTCTGATTG AGCATGGTGC TGTAGTTGAC 
CATACATGTA ACCAAGGTGC AACTGCACTC TGTATTGCAG CCCAGGAAGG GCACATTGAT 
GTTGTTCAGG TCTTATTAGA GCATGGTGCT GATCCAAACC ATGCTGATCA ATTTGGACGC 
ACTGCTATGC GTGTTGCAGC CAAAAATGGA CATTCTCAGA TAATTAAATT ATTAGAAAAA 
TATGGTGCAT CTAGTTTGAA TGGCTGTTCC CCATCTCCTG TTCACACAAT GGAGCAAAAA 
CCTCTACAGT CATTGTCTTC AAAAGTGCAG TCATTAACAA TTAAATCAAA TAGCTCTGGT 
AGTACTGGTG GAGGGGATAT GCAGCCTTCG TTACGTGGTT TACCTAATGG GCCTACTCAT 
GCTTTTAGTT CTCCTTCAGA ATCTCCAGAT TCTACAGTTG ACCGGCAGAA GTCATCACTG 
TCAAATAATT CCCTGAAAAG CTCAAAAAAT TCATCTTTGA GAACTACTTC ATCTACAGCA 
ACGGCTCAAA CAGTGCCAAT TGATAGCTTT CATAACTTGT CATTTACAGA ACAAATTCAG 
CAGCATTCAT TGCCACGCAG TAGAAGTCGA CAGTCAATTG TTTCCCCATC TTCCACAACA 
CAGTCCTTAG GACAGAGTCA TAATTCACCA AGTAGTGAAT TTGAGTGGAG TCAAGTAAAG 
CCCAGTTTGA AGTCAACTAA AGCAAGTAAA GGGGGGAAAT CAGAAAATTC TGCCAAGTCT 
GGATCAGCTG GGAAAAAAGC GAAACAAAGT AATTCTTCAC AGCCAAAGGT TTTAGAATAT 
GAAATGACTC AGTTTGATAG AAGAGGACCT ATAGCCAAAT CCGGGACTGC TGCACCACCT 
AAACAAATGC CAGCAGAATC TCAATGCAAA ATTATGATAC CTTCAGCTCA GCAGGAAATT 
GGTCGATCTC AACAGCAGTT TCTTATTCAC CAACAAAGTG GGGAACAGAA GAAGAGAAAT 
GGAATAATGA CAAATCCAAA TTATCATCTT CAGAGCAACC AGGTTTTTCT TGGTAGGGTT 
TCAGTCCCAC GAACAATGCA AGATAGAGGG CATCAGGAAG TGTTGGAGGG ATACCCTTCC 
TCAGAGACAG AATTAAGCCT TAAACAAGCT CTGAAGCTTC AGATTGAAGG TTCTGACCCT 
AGCTTCAACT ATAAAAAGGA AACACCATTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161354.1
CDS	301..4053
Translation	MKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_001167882.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167882.2

ORF Insert Sequence:

ATGAAGCCTC CCCAGCAAAG CCTATACCTG CTTGTTGATT CTGTTGATGA AGGGTGTAAC 
ATTACTGAAG GTGAACAAAC GTCTACCAGC TTATCTGGGA CTGTTGCAGC ACTTTTAGCT 
GGTCACCATG AGTTCTTTCC ACCATGGCTA TTGCTTCTCT GTTCTGCCCG AAAGCAGAGT 
AAGGCTGTTA CTAAAATGTT TACTGGTTTT CGAAAAATAA GTTTAGATGA CCTTCGGAAG 
GCATATATCG TCAAGGATGT TCAGCAGTAC ATTCTTCATC GTTTAGATCA AGAAGAAGCT 
TTGCGACAAC ACCTCACAAA AGAAACTGCA GAGATGTTAA ATCAACTGCA CATTAAAAGC 
AGTGGATGCT TTCTTTACCT AGAACGAGTT TTAGATGGAG TTGTAGAAAA TTTTATTATG 
TTAAGAGAAA TTCGTGACAT CCCAGGAACT CTAAATGGTT TATATCTCTG GCTGTGCCAA 
AGACTTTTTG TAAGAAAACA ATTTGCAAAG GTTCAGCCTA TTTTGAATGT GATTCTTGCA 
GCCTGCCGAC CTTTGACCAT AACGGAATTA TATCACGCAG TATGGACCAA AAACATGTCG 
TTAACTTTGG AAGATTTTCA ACGCAAGTTA GATATCCTCT CCAAACTTCT TGTTGATGGA 
CTAGGAAATA CAAAAATACT GTTTCATTAT AGTTTTGCCG AGTGGCTTCT GGATGTGAAA 
CACTGTACTC AGAAGTATTT ATGTAATGCA GCAGAAGGAC ACAGAATGTT GGCTATGAGT 
TATACCTGTC AAGCCAAGAA TTTAACACCA TTGGAAGCAC AAGAATTTGC ATTGCACTTA 
ATTAACTCAA ACTTACAATT AGAGACAGCG GAGTTAGCTC TGTGGATGAT ATGGAATGGT 
ACACCTGTCA GAGATTCCCT TTCTACTTTG ATACCCAAGG AACAAGAAGT GCTACAGCTG 
TTGGTTAAAG CTGGGGCTCA TGTCAACAGT GAAGACGATC GCACATCATG CATAGTTCGA 
CAAGCCTTAG AAAGAGAGGA TTCCATTCGG ACATTATTAG ATAATGGAGC TTCAGTAAAT 
CAGTGTGATT CAAATGGGAG AACATTATTG GCTAATGCTG CATATAGTGG CAGTCTTGAT 
GTAGTCAATT TACTTGTCTC TAGGGGAGCA GATTTAGAGA TAGAAGATGC TCATGGACAT 
ACACCACTCA CTCTAGCGGC TAGACAGGGA CATACCAAGG TGGTTAATTG TTTGATTGGG 
TGTGGAGCAA ATATTAATCA TACTGATCAA GATGGTTGGA CAGCATTAAG ATCTGCTGCT 
TGGGGTGGCC ATACTGAGGT AGTTTCTGCA CTACTTTATG CTGGCGTAAA AGTGGATTGT 
GCAGATGCTG ATAGCCGAAC AGCTTTGAGA GCAGCAGCAT GGGGAGGACA CGAGGATATT 
GTACTGAATT TGCTACAACA TGGCGCTGAA GTGAACAAAG CTGATAATGA AGGTAGAACT 
GCTTTGATAG CAGCAGCATA CATGGGACAT AGAGAGATTG TGGAACACCT ACTGGACCAT 
GGAGCAGAAG TAAATCATGA GGATGTTGAT GGCAGGACTG CACTCTCTGT AGCTGCACTT 
TGTGTGCCTG CAAGTAAAGG GCACGCATCA GTTGTTAGCC TTTTAATTGA TCGAGGTGCT 
GAAGTAGATC ATTGTGATAA AGATGGCATG ACTCCACTGC TGGTAGCTGC CTATGAAGGA 
CATGTTGATG TGGTTGACTT GCTTCTAGAA GGGGGAGCAG ATGTAGATCA CACAGATAAC 
AATGGCCGTA CACCCCTCTT AGCAGCAGCG TCTATGGGTC ATGCATCAGT TGTAAATACA 
CTTTTGTTTT GGGGTGCAGC TGTGGATAGT ATTGATAGTG AAGGTAGGAC AGTCCTCAGT 
ATAGCTTCAG CACAAGGAAA TGTTGAGGTG GTACGTACTC TACTGGATAG AGGGTTAGAT 
GAAAATCACA GAGATGATGC TGGATGGACA CCTTTGCACA TGGCAGCTTT TGAAGGGCAC 
AGATTGATAT GTGAAGCACT TATTGAACAA GGTGCTAGAA CAAATGAGAT TGACAATGAT 
GGACGAATCC CTTTCATATT AGCTTCACAA GAGGGTCATT ATGATTGTGT TCAAATATTA 
CTGGAAAACA AATCCAACAT TGATCAAAGA GGTTATGATG GAAGAAATGC ACTGCGGGTT 
GCTGCATTAG AAGGGCACAG GGACATTGTT GAATTGCTTT TTAGCCATGG TGCTGATGTT 
AACTGCAAAG ATGCTGATGG TCGGCCTACA CTTTATATCT TGGCCTTAGA AAATCAGCTT 
ACAATGGCCG AATATTTTTT AGAAAATGGT GCAAACGTAG AAGCAAGTGA TGCTGAAGGA 
AGGACAGCAC TTCATGTGTC TTGTTGGCAA GGCCATATGG AAATGGTGCA GGTCCTGATA 
GCATACCATG CTGACGTCAA TGCTGCAGAC AATGAAAAGC GCTCTGCTTT GCAGTCTGCA 
GCCTGGCAGG GCCATGTAAA AGTGGTTCAG CTTCTGATTG AGCATGGTGC TGTAGTTGAC 
CATACATGTA ACCAAGGTGC AACTGCACTC TGTATTGCAG CCCAGGAAGG GCACATTGAT 
GTTGTTCAGG TCTTATTAGA GCATGGTGCT GATCCAAACC ATGCTGATCA ATTTGGACGC 
ACTGCTATGC GTGTTGCAGC CAAAAATGGA CATTCTCAGA TAATTAAATT ATTAGAAAAA 
TATGGTGCAT CTAGTTTGAA TGGCTGTTCC CCATCTCCTG TTCACACAAT GGAGCAAAAA 
CCTCTACAGT CATTGTCTTC AAAAGTGCAG TCATTAACAA TTAAATCAAA TAGCTCTGGT 
AGTACTGGTG GAGGGGATAT GCAGCCTTCG TTACGTGGTT TACCTAATGG GCCTACTCAT 
GCTTTTAGTT CTCCTTCAGA ATCTCCAGAT TCTACAGTTG ACCGGCAGAA GTCATCACTG 
TCAAATAATT CCCTGAAAAG CTCAAAAAAT TCATCTTTGA GAACTACTTC ATCTACAGCA 
ACGGCTCAAA CAGTGCCAAT TGATAGCTTT CATAACTTGT CATTTACAGA ACAAATTCAG 
CAGCATTCAT TGCCACGCAG TAGAAGTCGA CAGTCAATTG TTTCCCCATC TTCCACAACA 
CAGTCCTTAG GACAGAGTCA TAATTCACCA AGTAGTGAAT TTGAGTGGAG TCAAGTAAAG 
CCCAGTTTGA AGTCAACTAA AGCAAGTAAA GGGGGGAAAT CAGAAAATTC TGCCAAGTCT 
GGATCAGCTG GGAAAAAAGC GAAACAAAGT AATTCTTCAC AGCCAAAGGT TTTAGAATAT 
GAAATGACTC AGTTTGATAG AAGAGGACCT ATAGCCAAAT CCGGGACTGC TGCACCACCT 
AAACAAATGC CAGCAGAATC TCAATGCAAA ATTATGATAC CTTCAGCTCA GCAGGAAATT 
GGTCGATCTC AACAGCAGTT TCTTATTCAC CAACAAAGTG GGGAACAGAA GAAGAGAAAT 
GGAATAATGA CAAATCCAAA TTATCATCTT CAGAGCAACC AGGTTTTTCT TGGTAGGGTT 
TCAGTCCCAC GAACAATGCA AGATAGAGGG CATCAGGAAG TGTTGGAGGG ATACCCTTCC 
TCAGAGACAG AATTAAGCCT TAAACAAGCT CTGAAGCTTC AGATTGAAGG TTCTGACCCT 
AGCTTCAACT ATAAAAAGGA AACACCATTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( NM_020337.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065070.1 Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.

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CloneID	OHu03351
Clone ID Related Accession (Same CDS sequence)	XM_017008471.1 , NM_020337.2 , NM_020337.3
Accession Version	NM_020337.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 50 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295135.1, AK291068.1, CX871714.1, AK127496.1, AB033049.2 and AA628892.1. On Nov 18, 2009 this sequence version replaced NM_020337.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK291068.1, SRR1660807.63981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065070.1
CDS	1039..5328
Translation	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSNCCNSAVNAPSLVMNSGNNASGVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPASLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_020337.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020337.2

ORF Insert Sequence:

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( NM_020337.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065070.1 Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03351
Clone ID Related Accession (Same CDS sequence)	XM_017008471.1 , NM_020337.2 , NM_020337.3
Accession Version	NM_020337.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 50 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295135.1, AK291068.1, CX871714.1, AK127496.1, AB033049.2 and AA628892.1. On Nov 22, 2018 this sequence version replaced NM_020337.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK291068.1, SRR1660807.63981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000504087.6/ ENSP00000425658.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065070.1
CDS	1039..5328
Translation	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSNCCNSAVNAPSLVMNSGNNASGVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPASLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	NM_020337.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020337.3

ORF Insert Sequence:

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD50 ( XM_017008471.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863960.1 Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu03351
Clone ID Related Accession (Same CDS sequence)	XM_017008471.1 , NM_020337.2 , NM_020337.3
Accession Version	XM_017008471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863960.1
CDS	1772..6061
Translation	MTNPWEEKVCKMAQTSLLQGKQFYCREWVFHKLQHCLQEKSNCCNSAVNAPSLVMNSGNNASGVSGKGAAWGVLLVGGPGSGKTALCTELLWPSSPASLQRGLHRQALAFHFCKAQDSDTLCVGGFIRGLVAQICRSGLLQGYEDKLRDPAVQSLLQPGECERNPAEAFKRCVLLPLLGMKPPQQSLYLLVDSVDEGCNITEGEQTSTSLSGTVAALLAGHHEFFPPWLLLLCSARKQSKAVTKMFTGFRKISLDDLRKAYIVKDVQQYILHRLDQEEALRQHLTKETAEMLNQLHIKSSGCFLYLERVLDGVVENFIMLREIRDIPGTLNGLYLWLCQRLFVRKQFAKVQPILNVILAACRPLTITELYHAVWTKNMSLTLEDFQRKLDILSKLLVDGLGNTKILFHYSFAEWLLDVKHCTQKYLCNAAEGHRMLAMSYTCQAKNLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVRDSLSTLIPKEQEVLQLLVKAGAHVNSEDDRTSCIVRQALEREDSIRTLLDNGASVNQCDSNGRTLLANAAYSGSLDVVNLLVSRGADLEIEDAHGHTPLTLAARQGHTKVVNCLIGCGANINHTDQDGWTALRSAAWGGHTEVVSALLYAGVKVDCADADSRTALRAAAWGGHEDIVLNLLQHGAEVNKADNEGRTALIAAAYMGHREIVEHLLDHGAEVNHEDVDGRTALSVAALCVPASKGHASVVSLLIDRGAEVDHCDKDGMTPLLVAAYEGHVDVVDLLLEGGADVDHTDNNGRTPLLAAASMGHASVVNTLLFWGAAVDSIDSEGRTVLSIASAQGNVEVVRTLLDRGLDENHRDDAGWTPLHMAAFEGHRLICEALIEQGARTNEIDNDGRIPFILASQEGHYDCVQILLENKSNIDQRGYDGRNALRVAALEGHRDIVELLFSHGADVNCKDADGRPTLYILALENQLTMAEYFLENGANVEASDAEGRTALHVSCWQGHMEMVQVLIAYHADVNAADNEKRSALQSAAWQGHVKVVQLLIEHGAVVDHTCNQGATALCIAAQEGHIDVVQVLLEHGADPNHADQFGRTAMRVAAKNGHSQIIKLLEKYGASSLNGCSPSPVHTMEQKPLQSLSSKVQSLTIKSNSSGSTGGGDMQPSLRGLPNGPTHAFSSPSESPDSTVDRQKSSLSNNSLKSSKNSSLRTTSSTATAQTVPIDSFHNLSFTEQIQQHSLPRSRSRQSIVSPSSTTQSLGQSHNSPSSEFEWSQVKPSLKSTKASKGGKSENSAKSGSAGKKAKQSNSSQPKVLEYEMTQFDRRGPIAKSGTAAPPKQMPAESQCKIMIPSAQQEIGRSQQQFLIHQQSGEQKKRNGIMTNPNYHLQSNQVFLGRVSVPRTMQDRGHQEVLEGYPSSETELSLKQALKLQIEGSDPSFNYKKETPL

Target ORF information:

RefSeq Version	XM_017008471.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008471.1

ORF Insert Sequence:

ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACCAGTTT ACTGCAAGGG 
AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAA 
AGTAACTGCT GCAATAGTGC TGTCAATGCA CCATCACTTG TAATGAATTC TGGGAATAAT 
GCTAGTGGTG TCTCTGGAAA GGGAGCTGCC TGGGGTGTGT TGTTGGTAGG AGGGCCTGGC 
AGTGGCAAGA CGGCCCTATG TACTGAACTC TTATGGCCAA GTTCACCTGC AAGTTTGCAG 
AGAGGTTTAC ATCGCCAAGC TTTGGCCTTT CATTTCTGCA AAGCCCAGGA CTCTGATACT 
TTGTGTGTTG GAGGGTTTAT TAGAGGTCTA GTAGCCCAGA TCTGCCGCAG TGGACTACTC 
CAAGGATATG AGGACAAGCT AAGGGATCCA GCAGTCCAAA GCCTCTTACA GCCTGGGGAG 
TGCGAGAGAA ACCCAGCCGA AGCATTTAAA AGGTGTGTTC TACTCCCTCT TCTGGGAATG 
AAGCCTCCCC AGCAAAGCCT ATACCTGCTT GTTGATTCTG TTGATGAAGG GTGTAACATT 
ACTGAAGGTG AACAAACGTC TACCAGCTTA TCTGGGACTG TTGCAGCACT TTTAGCTGGT 
CACCATGAGT TCTTTCCACC ATGGCTATTG CTTCTCTGTT CTGCCCGAAA GCAGAGTAAG 
GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA 
TATATCGTCA AGGATGTTCA GCAGTACATT CTTCATCGTT TAGATCAAGA AGAAGCTTTG 
CGACAACACC TCACAAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGCAGT 
GGATGCTTTC TTTACCTAGA ACGAGTTTTA GATGGAGTTG TAGAAAATTT TATTATGTTA 
AGAGAAATTC GTGACATCCC AGGAACTCTA AATGGTTTAT ATCTCTGGCT GTGCCAAAGA 
CTTTTTGTAA GAAAACAATT TGCAAAGGTT CAGCCTATTT TGAATGTGAT TCTTGCAGCC 
TGCCGACCTT TGACCATAAC GGAATTATAT CACGCAGTAT GGACCAAAAA CATGTCGTTA 
ACTTTGGAAG ATTTTCAACG CAAGTTAGAT ATCCTCTCCA AACTTCTTGT TGATGGACTA 
GGAAATACAA AAATACTGTT TCATTATAGT TTTGCCGAGT GGCTTCTGGA TGTGAAACAC 
TGTACTCAGA AGTATTTATG TAATGCAGCA GAAGGACACA GAATGTTGGC TATGAGTTAT 
ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCATT GCACTTAATT 
AACTCAAACT TACAATTAGA GACAGCGGAG TTAGCTCTGT GGATGATATG GAATGGTACA 
CCTGTCAGAG ATTCCCTTTC TACTTTGATA CCCAAGGAAC AAGAAGTGCT ACAGCTGTTG 
GTTAAAGCTG GGGCTCATGT CAACAGTGAA GACGATCGCA CATCATGCAT AGTTCGACAA 
GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGATA ATGGAGCTTC AGTAAATCAG 
TGTGATTCAA ATGGGAGAAC ATTATTGGCT AATGCTGCAT ATAGTGGCAG TCTTGATGTA 
GTCAATTTAC TTGTCTCTAG GGGAGCAGAT TTAGAGATAG AAGATGCTCA TGGACATACA 
CCACTCACTC TAGCGGCTAG ACAGGGACAT ACCAAGGTGG TTAATTGTTT GATTGGGTGT 
GGAGCAAATA TTAATCATAC TGATCAAGAT GGTTGGACAG CATTAAGATC TGCTGCTTGG 
GGTGGCCATA CTGAGGTAGT TTCTGCACTA CTTTATGCTG GCGTAAAAGT GGATTGTGCA 
GATGCTGATA GCCGAACAGC TTTGAGAGCA GCAGCATGGG GAGGACACGA GGATATTGTA 
CTGAATTTGC TACAACATGG CGCTGAAGTG AACAAAGCTG ATAATGAAGG TAGAACTGCT 
TTGATAGCAG CAGCATACAT GGGACATAGA GAGATTGTGG AACACCTACT GGACCATGGA 
GCAGAAGTAA ATCATGAGGA TGTTGATGGC AGGACTGCAC TCTCTGTAGC TGCACTTTGT 
GTGCCTGCAA GTAAAGGGCA CGCATCAGTT GTTAGCCTTT TAATTGATCG AGGTGCTGAA 
GTAGATCATT GTGATAAAGA TGGCATGACT CCACTGCTGG TAGCTGCCTA TGAAGGACAT 
GTTGATGTGG TTGACTTGCT TCTAGAAGGG GGAGCAGATG TAGATCACAC AGATAACAAT 
GGCCGTACAC CCCTCTTAGC AGCAGCGTCT ATGGGTCATG CATCAGTTGT AAATACACTT 
TTGTTTTGGG GTGCAGCTGT GGATAGTATT GATAGTGAAG GTAGGACAGT CCTCAGTATA 
GCTTCAGCAC AAGGAAATGT TGAGGTGGTA CGTACTCTAC TGGATAGAGG GTTAGATGAA 
AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGGCACAGA 
TTGATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATTGA CAATGATGGA 
CGAATCCCTT TCATATTAGC TTCACAAGAG GGTCATTATG ATTGTGTTCA AATATTACTG 
GAAAACAAAT CCAACATTGA TCAAAGAGGT TATGATGGAA GAAATGCACT GCGGGTTGCT 
GCATTAGAAG GGCACAGGGA CATTGTTGAA TTGCTTTTTA GCCATGGTGC TGATGTTAAC 
TGCAAAGATG CTGATGGTCG GCCTACACTT TATATCTTGG CCTTAGAAAA TCAGCTTACA 
ATGGCCGAAT ATTTTTTAGA AAATGGTGCA AACGTAGAAG CAAGTGATGC TGAAGGAAGG 
ACAGCACTTC ATGTGTCTTG TTGGCAAGGC CATATGGAAA TGGTGCAGGT CCTGATAGCA 
TACCATGCTG ACGTCAATGC TGCAGACAAT GAAAAGCGCT CTGCTTTGCA GTCTGCAGCC 
TGGCAGGGCC ATGTAAAAGT GGTTCAGCTT CTGATTGAGC ATGGTGCTGT AGTTGACCAT 
ACATGTAACC AAGGTGCAAC TGCACTCTGT ATTGCAGCCC AGGAAGGGCA CATTGATGTT 
GTTCAGGTCT TATTAGAGCA TGGTGCTGAT CCAAACCATG CTGATCAATT TGGACGCACT 
GCTATGCGTG TTGCAGCCAA AAATGGACAT TCTCAGATAA TTAAATTATT AGAAAAATAT 
GGTGCATCTA GTTTGAATGG CTGTTCCCCA TCTCCTGTTC ACACAATGGA GCAAAAACCT 
CTACAGTCAT TGTCTTCAAA AGTGCAGTCA TTAACAATTA AATCAAATAG CTCTGGTAGT 
ACTGGTGGAG GGGATATGCA GCCTTCGTTA CGTGGTTTAC CTAATGGGCC TACTCATGCT 
TTTAGTTCTC CTTCAGAATC TCCAGATTCT ACAGTTGACC GGCAGAAGTC ATCACTGTCA 
AATAATTCCC TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG 
GCTCAAACAG TGCCAATTGA TAGCTTTCAT AACTTGTCAT TTACAGAACA AATTCAGCAG 
CATTCATTGC CACGCAGTAG AAGTCGACAG TCAATTGTTT CCCCATCTTC CACAACACAG 
TCCTTAGGAC AGAGTCATAA TTCACCAAGT AGTGAATTTG AGTGGAGTCA AGTAAAGCCC 
AGTTTGAAGT CAACTAAAGC AAGTAAAGGG GGGAAATCAG AAAATTCTGC CAAGTCTGGA 
TCAGCTGGGA AAAAAGCGAA ACAAAGTAAT TCTTCACAGC CAAAGGTTTT AGAATATGAA 
ATGACTCAGT TTGATAGAAG AGGACCTATA GCCAAATCCG GGACTGCTGC ACCACCTAAA 
CAAATGCCAG CAGAATCTCA ATGCAAAATT ATGATACCTT CAGCTCAGCA GGAAATTGGT 
CGATCTCAAC AGCAGTTTCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAATGGA 
ATAATGACAA ATCCAAATTA TCATCTTCAG AGCAACCAGG TTTTTCTTGG TAGGGTTTCA 
GTCCCACGAA CAATGCAAGA TAGAGGGCAT CAGGAAGTGT TGGAGGGATA CCCTTCCTCA 
GAGACAGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAAGGTTC TGACCCTAGC 
TTCAACTATA AAAAGGAAAC ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retromer- and WASH-dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.
Journal of cell science130(2)382-395(2017 01)
Kvainickas A,Orgaz AJ,N?gele H,Diedrich B,Heesom KJ,Dengjel J,Cullen PJ,Steinberg F

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.
Journal of cell science127(Pt 22)4940-53(2014 Nov)
McGough IJ,Steinberg F,Gallon M,Yatsu A,Ohbayashi N,Heesom KJ,Fukuda M,Cullen PJ

A physical interaction network of dengue virus and human proteins.
Molecular & cellular proteomics : MCP10(12)M111.012187(2011 Dec)
Khadka S,Vangeloff AD,Zhang C,Siddavatam P,Heaton NS,Wang L,Sengupta R,Sahasrabudhe S,Randall G,Gribskov M,Kuhn RJ,Perera R,LaCount DJ

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ANKRD50 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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