ANKRD50 cDNA ORF clone, Bos taurus(cattle)

The following ANKRD50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa38567 NM_001205949.2
Latest version!
Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27703 XM_005217603.4
Latest version!
Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27703 XM_005217604.4
Latest version!
Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27703 XM_010813781.3
Latest version!
Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa27703 XM_005217603.3 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa38567 NM_001205949.1 Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27703 XM_005217604.3 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa27703 XM_010813781.2 Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa38567
    Clone ID Related Accession (Same CDS sequence) NM_001205949.1 , NM_001205949.2
    Accession Version NM_001205949.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02044718.1. On Apr 20, 2011 this sequence version replaced XM_606363.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403654, SAMN02403655 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
    AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG
    ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT
    GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC
    AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG
    AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT
    TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC
    CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA
    TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG
    AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT
    ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT
    CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA
    GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA
    TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG
    CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC
    GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG
    AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA
    CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC
    TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA
    ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT
    GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC
    TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT
    ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT
    AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA
    CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG
    GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG
    GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG
    TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG
    GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA
    CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT
    GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG
    GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA
    GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG
    CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT
    TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA
    GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT
    GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA
    GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT
    GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT
    GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT
    TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA
    GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA
    AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA
    TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG
    CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG
    GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT
    GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC
    TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA
    ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG
    ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC
    TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC
    TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC
    ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC
    GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT
    GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT
    GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT
    CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC
    ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA
    TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA
    AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG
    GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG
    CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG
    TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC
    AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG
    TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA
    ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG
    CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT
    CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT
    ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA
    GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG
    GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC
    TTCAACTATA AAAAGGAAAC ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192878.1
    CDS1..4290
    Translation

    Target ORF information:

    RefSeq Version NM_001205949.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205949.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
    AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG
    ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT
    GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC
    AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG
    AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT
    TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC
    CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA
    TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG
    AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT
    ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT
    CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA
    GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA
    TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG
    CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC
    GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG
    AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA
    CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC
    TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA
    ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT
    GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC
    TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT
    ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT
    AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA
    CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG
    GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG
    GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG
    TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG
    GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA
    CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT
    GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG
    GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA
    GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG
    CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT
    TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA
    GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT
    GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA
    GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT
    GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT
    GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT
    TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA
    GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA
    AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA
    TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG
    CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG
    GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT
    GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC
    TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA
    ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG
    ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC
    TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC
    TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC
    ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC
    GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT
    GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT
    GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT
    CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC
    ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA
    TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA
    AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG
    GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG
    CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG
    TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC
    AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG
    TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA
    ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG
    CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT
    CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT
    ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA
    GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG
    GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC
    TTCAACTATA AAAAGGAAAC ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38567
    Clone ID Related Accession (Same CDS sequence) NM_001205949.1 , NM_001205949.2
    Accession Version NM_001205949.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-20
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Dec 21, 2018 this sequence version replaced NM_001205949.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721698.3619.1, SRR5721694.3415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
    AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG
    ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT
    GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC
    AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG
    AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT
    TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC
    CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA
    TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG
    AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT
    ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT
    CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA
    GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA
    TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG
    CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC
    GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG
    AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA
    CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC
    TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA
    ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT
    GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC
    TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT
    ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT
    AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA
    CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG
    GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG
    GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG
    TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG
    GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA
    CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT
    GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG
    GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA
    GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG
    CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT
    TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA
    GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT
    GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA
    GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT
    GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT
    GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT
    TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA
    GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA
    AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA
    TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG
    CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG
    GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT
    GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC
    TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA
    ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG
    ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC
    TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC
    TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC
    ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC
    GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT
    GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT
    GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT
    CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC
    ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA
    TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA
    AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG
    GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG
    CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG
    TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC
    AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG
    TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA
    ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG
    CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT
    CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT
    ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA
    GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG
    GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC
    TTCAACTATA AAAAGGAAAC ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192878.1
    CDS1..4290
    Translation

    Target ORF information:

    RefSeq Version NM_001205949.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205949.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTAATC CTTGGGAAGA GAAAGTCTGC AAAATGGCTC AAACGAGTTT ACTGCAGGGG 
    AAGCAGTTTT ACTGTAGGGA GTGGGTTTTC CACAAGCTTC AGCATTGCCT CCAGGAGAAG
    ACTAACTGCT GCAACAGTGC TGTCAACACA CCACCCCTTG TAATGAATTC TGGGAATAAT
    GCTAGTGTTG TCTCTGGGAA AGGAGCTGCC TGGGGTGTAT TATTGGTAGG AGGGCCTGGC
    AGTGGCAAGA CAGCCTTATG CACTGAACTC TTGTGGCCAA GTTCACCTAC AAGCTTGCAG
    AGAGGTTTAC ATCGCCAGGC GTTGGCCTTT CATTTCTGCA AAGCGCAGGA CTCTGATACT
    TTGTGTGTTG GAGGGTTTAT TAGAGGTCTG GTTGCTCAGA TCTGCCGTAG CGGACTACTC
    CAAGGATATG AGGACAAACT AAGGGATCCA GCAGTTCAAA GTCTCCTACA GCCCGGGGAA
    TGTGAGAGAA ACCCAGCGGA AGCATTTAAA AGGTGCGTCC TGCTCCCTCT TTTGGGAATG
    AAGCCACCCC AGCAAAGCCT CTATCTTCTT GTGGATTCTG TGGACGAAGG GTGTAACATT
    ACTGAAGGTG AACAGACGTC CACCAGCTTA TCTGGGACTG TGGCAGAGCT TTTGGCTGGT
    CACCACGAGT TCTTTCCACC GTGGCTATTG CTTCTCTGTT CTGCCCGAAA ACAGAGCAAA
    GCTGTTACTA AAATGTTTAC TGGTTTTCGA AAAATAAGTT TAGATGACCT TCGGAAGGCA
    TATATCGTTA AGGATGTTCA GCAGTACATC CTTCATCGTT TAGATCAAGA AGAAGCTTTG
    CGACAACACC TCACGAAAGA AACTGCAGAG ATGTTAAATC AACTGCACAT TAAAAGTAGC
    GGGTGCTTTC TTTATCTAGA ACGAGTTCTC GATGGAGTTG TAGAAAATTT TATTATGTTG
    AGAGAGATTC GTGACATCCC AGGAACTCTA AATGGTCTGT ATCTCTGGCT GTGTCAAAGA
    CTTTTCGTAA GAAAACAGTT TGCAAAGGTT CAGCCTATCT TGAATGTGAT TCTTGCAGCC
    TGCCGGCCTC TGACCATAAC AGAATTATAT CATGCAGTAT GGACAAAAAA TATGTCATTA
    ACCTTGGAAG ACTTTCAACG CAAGCTAGAT GTCCTTTCCA AACTTCTTGT TGATGGACTT
    GGAAATACTA AAATACTGTT TCACTACAGT TTTGCAGAGT GGCTCCTGGA TGTGAAACAC
    TGCACTCAGA AGTATTTATG CAATGCAGCA GAAGGACACC GAATGTTGGC TATGAGTTAT
    ACCTGTCAAG CCAAGAATTT AACACCATTG GAAGCACAAG AATTTGCGTT GCACTTAATT
    AATTCGAACT TACAGTTAGA GACAGCTGAG TTAGCTCTGT GGATGATATG GAATGGCACA
    CCTGTCAGAG ACTCCCTATC TACTTTAATA CCCAAGGAAC AAGAAGTGCT GCAGCTGTTG
    GTGAAAGCTG GCGCTCATGT CAACAGCGAA GATGATCGCA CATCATGCAT CGTCCGGCAG
    GCCTTAGAAA GAGAGGATTC CATTCGGACA TTATTAGACA ATGGAGCTTC AGTAAATCAG
    TGTGATTCGA ATGGGAGAAC GTTACTGGCT AATGCGGCTT ACAGTGGCAA CCTCGATGTG
    GTGAATTTAC TGGTCTCTAG GGGAGCAGAT TTAGAAATAG AAGATGCTCA CGGACACACA
    CCGCTTACCC TGGCTGCTCG ACAAGGACAT ACCAAGGTGG TTAATTGTCT GATTGGTTGT
    GGAGCAAATA TTAATCACAC CGATCAGGAT GGCTGGACAG CATTGAGATC CGCGGCTTGG
    GGTGGTCACA CCGAGGTTGT GTCTGCACTG CTTTATGCTG GCGTAAAAGT GGACTGTGCA
    GATGCCGACA GCCGAACAGC TCTGAGAGCA GCAGCCTGGG GAGGACATGA GGATATTGTG
    CTGAACTTGC TCCAGCATGG TGCTGAAGTC AACAAAGCTG ACAATGAAGG CCGAACTGCT
    TTGATAGCGG CTGCCTACAT GGGACATAGA GAGATTGTGG AACACCTGCT GGATCACGGA
    GCAGAAGTCA ATCATGAGGA TGTCGACGGC AGGACTGCAC TCTCTGTGGC TGCACTTTGT
    GTTCCTGCAA GTAAAGGACA TGCATCGGTC GTTAGCCTTC TGATTGATCG AGGTGCTGAA
    GTTGATCACT GCGATAAAGA TGGCATGACG CCCCTACTGG TGGCTGCCTA TGAAGGACAT
    GTCGATGTGG TTGACTTGCT TCTAGAGGGG GGAGCTGATG TCGACCACAC GGATAACAAT
    GGGCGTACAC CCCTCCTAGC TGCCGCGTCT ATGGGTCATG CCTCAGTTGT AAACACGCTT
    TTGTTTTGGG GTGCTGCTGT GGACAGCATT GACAGTGAAG GGAGAACAGT GCTTAGCATA
    GCTTCAGCCC AAGGAAACGT TGAAGTAGTC CGTACTCTGC TGGACCGGGG CTTAGATGAA
    AATCACAGAG ATGATGCTGG ATGGACACCT TTGCACATGG CAGCTTTTGA AGGCCACAGA
    TTAATATGTG AAGCACTTAT TGAACAAGGT GCTAGAACAA ATGAGATCGA CAACGATGGG
    CGGATACCTT TCATATTAGC TTCACAAGAG GGTCATTATG ACTGTGTTCA AATATTACTG
    GAGAATAAAT CCAACATTGA TCAGAGAGGT TATGATGGAA GAAATGCTCT GCGGGTCGCT
    GCATTAGAAG GACACCGGGA CATTGTTGAA TTGCTTTTTA GCCATGGAGC TGATGTTAAC
    TACAAAGATG CTGATGGTAG GCCAACACTT TATATTTTGG CCTTAGAAAA TCAGCTTACA
    ATGGCTGAGT ATTTTTTGGA AAATGGTGCA AATGTGGAAG CAAGTGATGC TGAAGGCAGG
    ACAGCACTTC ATGTCTCGTG CTGGCAAGGC CACTTGGAAA TGGTGCAGGT TCTGATCACC
    TATCATGCTG ATGTCAATGC TGCTGACAAT GAAAAGCGCT CTGCACTGCA GTCTGCAGCC
    TGGCAGGGCC ACGTCAAAGT GGTGCAGCTT CTCATTGAAC ATGGCGCCGT GGTGGACCAC
    ACGTGTAATC AGGGCGCCAC CGCGCTCTGC ATTGCAGCCC AGGAAGGACA CATTGACGTC
    GTGCAGGTTT TATTAGAGCA CGGTGCTGAT CCAAACCACG CTGATCAATT TGGACGTACT
    GCTATGCGAG TTGCAGCCAA AAACGGACAC TCTCAAATAA TTAAATTATT AGAAAAATAT
    GGTGCATCCA GCTTGAATGG CTGTTCTCCA TCTCCTGTTC ACACGATGGA GCAAAAGCCT
    CTACAGTCAG TGTCATCGAA GATGCAGTCC CTAACAATTA AATCAAACAG CTCAGGCAGC
    ACTGGTGGTG GGGAAGCGCA GCCTTCGTTG CGCAGTTTGC CAAACGGGCC AGCTCATGCA
    TTCAGTTCTC CTTCAGAATC GCCTGATTCG ACAGTTGATC GGCAAAAGTC ATCATTGTCA
    AATAATTCCT TGAAAAGCTC AAAAAATTCA TCTTTGAGAA CTACTTCATC TACAGCAACG
    GCTCAAACAG TGCCAATCGA CAGTTTCCAC AGCTTGTCAT TTACAGAACA AATTCAGCAG
    CACTCGTTGC CGCGCAGTAG AAGTCGCCAG TCGATTGTCT CCCCGTCTTC CACAACACAG
    TCCTTGGGCC AGAGCCATCA TTCACCGAGT AGTGAGTTTG AGTGGAGTCA AGTCAAGCCC
    AGCTTGAAGT CAACAAAAAC GAATAAAGGG GGGAAATCAG AAAATTCCAA CAAATCTGGG
    TCAGCTGGAA AAAAAGCTAA ACAAAATAAT TCTTCACAGC CGAAGGTCCT AGAATATGAA
    ATGACTCAGT TTGATAAAAG AGGACCCACA GCCAAATCTG GGACCAGTGC ACCATCTAAG
    CAGATGCCAG CAGATTCGCA GTGTAAAATC ATGATTCCTT CAGCTCAGCC AGAAATAGGT
    CGATCTCAAC AGCAGTTCCT TATTCACCAA CAAAGTGGGG AACAGAAGAA GAGAAACGGT
    ATAATGACAA ATCCAAATTA TCATCTGCAG AGCAACCAGG TTTTTCTCGG TAGGGTTTCA
    GTCCCACGGA CAATACAAGA CAGAGGGCAT CAGGAAGTGT TAGAAGGATA CCCTTCCTCG
    GAGACGGAAT TAAGCCTTAA ACAAGCTCTG AAGCTTCAGA TTGAGGGTTC TGACCCTAGC
    TTCAACTATA AAAAGGAAAC ACCATTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_005217603.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217603.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217660.1
    CDS686..5008
    Translation

    Target ORF information:

    RefSeq Version XM_005217603.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217603.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_005217604.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217604.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217661.1
    CDS739..5061
    Translation

    Target ORF information:

    RefSeq Version XM_005217604.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217604.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_010813781.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104449.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812083.1
    CDS883..5205
    Translation

    Target ORF information:

    RefSeq Version XM_010813781.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_005217603.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217603.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217660.1
    CDS661..4983
    Translation

    Target ORF information:

    RefSeq Version XM_005217603.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217603.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_005217604.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217604.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217661.1
    CDS714..5036
    Translation

    Target ORF information:

    RefSeq Version XM_005217604.4
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217604.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27703
    Clone ID Related Accession (Same CDS sequence) XM_005217603.3 , XM_005217604.3 , XM_010813781.2 , XM_005217603.4 , XM_005217604.4 , XM_010813781.3
    Accession Version XM_010813781.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ankyrin repeat domain-containing protein 50 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812083.1
    CDS943..5265
    Translation

    Target ORF information:

    RefSeq Version XM_010813781.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ankyrin repeat domain 50 (ANKRD50), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCTAGCAA ATGTTGATGG TAAGAAACCT ATTATGACTA ATCCTTGGGA AGAGAAAGTC 
    TGCAAAATGG CTCAAACGAG TTTACTGCAG GGGAAGCAGT TTTACTGTAG GGAGTGGGTT
    TTCCACAAGC TTCAGCATTG CCTCCAGGAG AAGACTAACT GCTGCAACAG TGCTGTCAAC
    ACACCACCCC TTGTAATGAA TTCTGGGAAT AATGCTAGTG TTGTCTCTGG GAAAGGAGCT
    GCCTGGGGTG TATTATTGGT AGGAGGGCCT GGCAGTGGCA AGACAGCCTT ATGCACTGAA
    CTCTTGTGGC CAAGTTCACC TACAAGCTTG CAGAGAGGTT TACATCGCCA GGCGTTGGCC
    TTTCATTTCT GCAAAGCGCA GGACTCTGAT ACTTTGTGTG TTGGAGGGTT TATTAGAGGT
    CTGGTTGCTC AGATCTGCCG TAGCGGACTA CTCCAAGGAT ATGAGGACAA ACTAAGGGAT
    CCAGCAGTTC AAAGTCTCCT ACAGCCCGGG GAATGTGAGA GAAACCCAGC GGAAGCATTT
    AAAAGGTGCG TCCTGCTCCC TCTTTTGGGA ATGAAGCCAC CCCAGCAAAG CCTCTATCTT
    CTTGTGGATT CTGTGGACGA AGGGTGTAAC ATTACTGAAG GTGAACAGAC GTCCACCAGC
    TTATCTGGGA CTGTGGCAGA GCTTTTGGCT GGTCACCACG AGTTCTTTCC ACCGTGGCTA
    TTGCTTCTCT GTTCTGCCCG AAAACAGAGC AAAGCTGTTA CTAAAATGTT TACTGGTTTT
    CGAAAAATAA GTTTAGATGA CCTTCGGAAG GCATATATCG TTAAGGATGT TCAGCAGTAC
    ATCCTTCATC GTTTAGATCA AGAAGAAGCT TTGCGACAAC ACCTCACGAA AGAAACTGCA
    GAGATGTTAA ATCAACTGCA CATTAAAAGT AGCGGGTGCT TTCTTTATCT AGAACGAGTT
    CTCGATGGAG TTGTAGAAAA TTTTATTATG TTGAGAGAGA TTCGTGACAT CCCAGGAACT
    CTAAATGGTC TGTATCTCTG GCTGTGTCAA AGACTTTTCG TAAGAAAACA GTTTGCAAAG
    GTTCAGCCTA TCTTGAATGT GATTCTTGCA GCCTGCCGGC CTCTGACCAT AACAGAATTA
    TATCATGCAG TATGGACAAA AAATATGTCA TTAACCTTGG AAGACTTTCA ACGCAAGCTA
    GATGTCCTTT CCAAACTTCT TGTTGATGGA CTTGGAAATA CTAAAATACT GTTTCACTAC
    AGTTTTGCAG AGTGGCTCCT GGATGTGAAA CACTGCACTC AGAAGTATTT ATGCAATGCA
    GCAGAAGGAC ACCGAATGTT GGCTATGAGT TATACCTGTC AAGCCAAGAA TTTAACACCA
    TTGGAAGCAC AAGAATTTGC GTTGCACTTA ATTAATTCGA ACTTACAGTT AGAGACAGCT
    GAGTTAGCTC TGTGGATGAT ATGGAATGGC ACACCTGTCA GAGACTCCCT ATCTACTTTA
    ATACCCAAGG AACAAGAAGT GCTGCAGCTG TTGGTGAAAG CTGGCGCTCA TGTCAACAGC
    GAAGATGATC GCACATCATG CATCGTCCGG CAGGCCTTAG AAAGAGAGGA TTCCATTCGG
    ACATTATTAG ACAATGGAGC TTCAGTAAAT CAGTGTGATT CGAATGGGAG AACGTTACTG
    GCTAATGCGG CTTACAGTGG CAACCTCGAT GTGGTGAATT TACTGGTCTC TAGGGGAGCA
    GATTTAGAAA TAGAAGATGC TCACGGACAC ACACCGCTTA CCCTGGCTGC TCGACAAGGA
    CATACCAAGG TGGTTAATTG TCTGATTGGT TGTGGAGCAA ATATTAATCA CACCGATCAG
    GATGGCTGGA CAGCATTGAG ATCCGCGGCT TGGGGTGGTC ACACCGAGGT TGTGTCTGCA
    CTGCTTTATG CTGGCGTAAA AGTGGACTGT GCAGATGCCG ACAGCCGAAC AGCTCTGAGA
    GCAGCAGCCT GGGGAGGACA TGAGGATATT GTGCTGAACT TGCTCCAGCA TGGTGCTGAA
    GTCAACAAAG CTGACAATGA AGGCCGAACT GCTTTGATAG CGGCTGCCTA CATGGGACAT
    AGAGAGATTG TGGAACACCT GCTGGATCAC GGAGCAGAAG TCAATCATGA GGATGTCGAC
    GGCAGGACTG CACTCTCTGT GGCTGCACTT TGTGTTCCTG CAAGTAAAGG ACATGCATCG
    GTCGTTAGCC TTCTGATTGA TCGAGGTGCT GAAGTTGATC ACTGCGATAA AGATGGCATG
    ACGCCCCTAC TGGTGGCTGC CTATGAAGGA CATGTCGATG TGGTTGACTT GCTTCTAGAG
    GGGGGAGCTG ATGTCGACCA CACGGATAAC AATGGGCGTA CACCCCTCCT AGCTGCCGCG
    TCTATGGGTC ATGCCTCAGT TGTAAACACG CTTTTGTTTT GGGGTGCTGC TGTGGACAGC
    ATTGACAGTG AAGGGAGAAC AGTGCTTAGC ATAGCTTCAG CCCAAGGAAA CGTTGAAGTA
    GTCCGTACTC TGCTGGACCG GGGCTTAGAT GAAAATCACA GAGATGATGC TGGATGGACA
    CCTTTGCACA TGGCAGCTTT TGAAGGCCAC AGATTAATAT GTGAAGCACT TATTGAACAA
    GGTGCTAGAA CAAATGAGAT CGACAACGAT GGGCGGATAC CTTTCATATT AGCTTCACAA
    GAGGGTCATT ATGACTGTGT TCAAATATTA CTGGAGAATA AATCCAACAT TGATCAGAGA
    GGTTATGATG GAAGAAATGC TCTGCGGGTC GCTGCATTAG AAGGACACCG GGACATTGTT
    GAATTGCTTT TTAGCCATGG AGCTGATGTT AACTACAAAG ATGCTGATGG TAGGCCAACA
    CTTTATATTT TGGCCTTAGA AAATCAGCTT ACAATGGCTG AGTATTTTTT GGAAAATGGT
    GCAAATGTGG AAGCAAGTGA TGCTGAAGGC AGGACAGCAC TTCATGTCTC GTGCTGGCAA
    GGCCACTTGG AAATGGTGCA GGTTCTGATC ACCTATCATG CTGATGTCAA TGCTGCTGAC
    AATGAAAAGC GCTCTGCACT GCAGTCTGCA GCCTGGCAGG GCCACGTCAA AGTGGTGCAG
    CTTCTCATTG AACATGGCGC CGTGGTGGAC CACACGTGTA ATCAGGGCGC CACCGCGCTC
    TGCATTGCAG CCCAGGAAGG ACACATTGAC GTCGTGCAGG TTTTATTAGA GCACGGTGCT
    GATCCAAACC ACGCTGATCA ATTTGGACGT ACTGCTATGC GAGTTGCAGC CAAAAACGGA
    CACTCTCAAA TAATTAAATT ATTAGAAAAA TATGGTGCAT CCAGCTTGAA TGGCTGTTCT
    CCATCTCCTG TTCACACGAT GGAGCAAAAG CCTCTACAGT CAGTGTCATC GAAGATGCAG
    TCCCTAACAA TTAAATCAAA CAGCTCAGGC AGCACTGGTG GTGGGGAAGC GCAGCCTTCG
    TTGCGCAGTT TGCCAAACGG GCCAGCTCAT GCATTCAGTT CTCCTTCAGA ATCGCCTGAT
    TCGACAGTTG ATCGGCAAAA GTCATCATTG TCAAATAATT CCTTGAAAAG CTCAAAAAAT
    TCATCTTTGA GAACTACTTC ATCTACAGCA ACGGCTCAAA CAGTGCCAAT CGACAGTTTC
    CACAGCTTGT CATTTACAGA ACAAATTCAG CAGCACTCGT TGCCGCGCAG TAGAAGTCGC
    CAGTCGATTG TCTCCCCGTC TTCCACAACA CAGTCCTTGG GCCAGAGCCA TCATTCACCG
    AGTAGTGAGT TTGAGTGGAG TCAAGTCAAG CCCAGCTTGA AGTCAACAAA AACGAATAAA
    GGGGGGAAAT CAGAAAATTC CAACAAATCT GGGTCAGCTG GAAAAAAAGC TAAACAAAAT
    AATTCTTCAC AGCCGAAGGT CCTAGAATAT GAAATGACTC AGTTTGATAA AAGAGGACCC
    ACAGCCAAAT CTGGGACCAG TGCACCATCT AAGCAGATGC CAGCAGATTC GCAGTGTAAA
    ATCATGATTC CTTCAGCTCA GCCAGAAATA GGTCGATCTC AACAGCAGTT CCTTATTCAC
    CAACAAAGTG GGGAACAGAA GAAGAGAAAC GGTATAATGA CAAATCCAAA TTATCATCTG
    CAGAGCAACC AGGTTTTTCT CGGTAGGGTT TCAGTCCCAC GGACAATACA AGACAGAGGG
    CATCAGGAAG TGTTAGAAGG ATACCCTTCC TCGGAGACGG AATTAAGCCT TAAACAAGCT
    CTGAAGCTTC AGATTGAGGG TTCTGACCCT AGCTTCAACT ATAAAAAGGA AACACCATTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.