top3b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following top3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa01709 NM_001126919.3
Latest version!
Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa01723 NM_001195572.2
Latest version!
Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31826 XM_012956162.2
Latest version!
Xenopus tropicalis topoisomerase (DNA) III beta (top3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa47578 XM_012956162.3
Latest version!
Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa01709
    Clone ID Related Accession (Same CDS sequence) NM_001126919.3
    Accession Version NM_001126919.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2538bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-beta-1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC161096.1, CX333480.2, DT436561.1 and DT436560.1. On Aug 9, 2013 this sequence version replaced NM_001126919.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC161096.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGACAG TACTCATGGT TGCAGAGAAG CCTTCATTGG CACAGTCAAT CGCAAAGATT 
    CTTTCTAGAG GGAGTGCCTC CTCTCGTAAA GGACTGAATG GTGCGTGCTC TGTTCATGAA
    TACACAGGAT CATTCATGGG ACAGACTGTG CGCTTCAAGA TGACATCTGT CTGTGGTCAC
    GTTATGAGCT TGGACTTTAT AGGGAAATAC AACAACTGGG ACAAAGTGGA TCCAGCAGAG
    CTCTTTAGCA AAGCACCTAC AGAGAAGAAA GAAGCCAACC CAAAACTCAG TATGGTCAAA
    TTCCTTCAGG TGGAAGGACG TGGTTGTGAT TATGTTGTTC TTTGGCTGGA TTGTGATAAA
    GAAGGAGAAA ATATTTGCTT TGAGGTTCTA GATGCTATAC ATCCTGTAAT GAACAGAGCA
    CATGGCGGGG AGCGCACAGT ATACCGGGCA AAGTTCAGCT CCATTACTGA TACAGACATC
    CTAAATGCAA TGAACCGACT TGGGGAGCCC AATAAAAATG AAGCACTGTC AGTCGATGCC
    AGGTTTCAGA CAAAGTATTT TCAAGGAAAA TACGGCAACC TAGACAGCTC TCTGATCTCC
    TTTGGACCGT GTCAGACACC AACACTTGGA TTTTGTGTGG AAAGACATGA TAAGATCCAG
    TCTTTCAAAC CAGAGACTTA TTGGGTCCTT CAAGTAAAGG TGAGCCGTGG ACAGGAAAAC
    CCACTGATAC TAGATTGGGA TCGGGTGCGA GTCTTTGACA GAGAGATTGC TCAGATGTTC
    ATAAATATCA CCAAGACTGC AAAGGAGGCA CAGGTAGAGT CTGTAAGCAA GAAGGAGAAG
    GCCAAACAAA GGCCGCAAGC ATTAAACACT GTGGAAATGC TTCGTGTTGC TAGTTCTGCA
    CTGGGAATGG GTCCTCAACA TACAATGCAA ATTGCAGAGC GCCTGTACAC CCAAGGTTAC
    ATCAGCTATC CCCGAACAGA AACCACCCAC TATCCTGAAA ACTTTGACCT GAAAGGAACG
    CTTCGGCAGC AAGCTAACAA CCCTTTCTGG GCCGACATGG TTAAAACTTT GCTTGCTGAG
    GGAATCAACC GCCCTAGAAA AGGAACTGAT GCCGGAGACC ATCCACCAAT TACACCGATG
    CGTTCCGCTA CAGAGGCAGA ACTTGGTGGT GATGCTTGGA GACTGTATGA GTACATCACT
    CGGCACTTCA TTGCCACCAT CAGTCATGAC TGTAAATACC TTCAGACCAG TATTGCCTTC
    AGTGTGGGAC CTGAGAAGTT TACCACCACT TGCAAGGAGG TGATCTCACC AGGATTCACA
    GAAATTATGC CCTGGCAAGG TATTCCAAAG GAAGAGAGTT TCCAGCCGTG CGAAAGAGGA
    GACAAGTTCA GTGTGGATGA GATGAAACTG ATGGAAAAAC AGACATGCCC TCCAGACTAC
    TTGACTGAAG CTGAGCTCAT CTCTTTAATG GAGAGGCATG GCATTGGAAC TGATGCCAGC
    ATTCCAGTGC ACATTAATAA TATCTGCCAG CGCAATTATG TGACAGTGGA GAGTGGGCGC
    AGACTGAAGC CTACTAGCCT GGGCATTGTG TTGGTGCATG GATATTATAA GATAGATCCG
    GAACTGGTGC TTCCAACCAT TCGCAGTGCA GTGGAGAAGC AGCTCAACTT GATCGCTCAG
    GGGAAGGCAA ATTTCCAGCA AGTCCTGGAT CACACCTTGG ATATCTTTAA GAGGAAATTC
    CACTACTTTG TGGATTCAAT AGCAGGGATG GATGAGTTGA TGGAAGTATC ATTCTCACCT
    CTGGCTGCAA CTGGCAAACC ACTGTCTCGC TGTGGAAAAT GTCATCGATT TATGAAATAT
    ATTCAGGCCA AGCCTAGCCG CCTCCACTGC TCTCACTGTG ATGAAACCTA TAGTCTTCCT
    CAAAATGGCA CAATCAAACT GTACAAAGAA CTGCGCTGCC CACTAGATGA TTTTGAACTG
    GTTCTGTGGT CATCAGGGTC CCGAGGGAAA AGCTACCCTC TGTGTCCATA CTGCTACAAT
    CATGCCCCTT TTAGGGACAT GAAAAAAGGG ATGGGCTGCA ATGAGTGCAC ACATCCAACC
    TGCCAGCACT CACTTAGCAT GCTGGGAATT GCTCAGTGTG TTGAGTGTGA AAGTGGGGTA
    ATGGTCCTTG ACTCCACCTC TGGTCCAAAA TGGAAGATGT CCTGCAATAA ATGCAATGTC
    ATTGTGCACT TCTTTGAGAA TGCACATCGT GTGCGTGTAT CTGCAGAGAC TTGTGATGCA
    TGCGATGCTG CAGTGGTCCA TGTCGACTTC AACAAGGCAA AGTGTCCTCT ACCTGCAGAT
    CAAACACAGC ACGCTGGCTG TGTGTTCTGT GACCCTGTAT TTCAGGACCT AGTTGAGCTA
    AAACATGCCA CAATGAAACA TCCCATGCAC AAGGGTGGGC AGGGAAGAAG GCAAGGTAGA
    GGAAGAGGCA GGGGAAGAAG GGCAAACCCC AAAAAACCCA AGGACAAAAT GGCAGCCCTA
    GCTGCCTACT TTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120391.2
    CDS196..2733
    Translation

    Target ORF information:

    RefSeq Version NM_001126919.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001126919.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGACAG TACTCATGGT TGCAGAGAAG CCTTCATTGG CACAGTCAAT CGCAAAGATT 
    CTTTCTAGAG GGAGTGCCTC CTCTCGTAAA GGACTGAATG GTGCGTGCTC TGTTCATGAA
    TACACAGGAT CATTCATGGG ACAGACTGTG CGCTTCAAGA TGACATCTGT CTGTGGTCAC
    GTTATGAGCT TGGACTTTAT AGGGAAATAC AACAACTGGG ACAAAGTGGA TCCAGCAGAG
    CTCTTTAGCA AAGCACCTAC AGAGAAGAAA GAAGCCAACC CAAAACTCAG TATGGTCAAA
    TTCCTTCAGG TGGAAGGACG TGGTTGTGAT TATGTTGTTC TTTGGCTGGA TTGTGATAAA
    GAAGGAGAAA ATATTTGCTT TGAGGTTCTA GATGCTATAC ATCCTGTAAT GAACAGAGCA
    CATGGCGGGG AGCGCACAGT ATACCGGGCA AAGTTCAGCT CCATTACTGA TACAGACATC
    CTAAATGCAA TGAACCGACT TGGGGAGCCC AATAAAAATG AAGCACTGTC AGTCGATGCC
    AGGTTTCAGA CAAAGTATTT TCAAGGAAAA TACGGCAACC TAGACAGCTC TCTGATCTCC
    TTTGGACCGT GTCAGACACC AACACTTGGA TTTTGTGTGG AAAGACATGA TAAGATCCAG
    TCTTTCAAAC CAGAGACTTA TTGGGTCCTT CAAGTAAAGG TGAGCCGTGG ACAGGAAAAC
    CCACTGATAC TAGATTGGGA TCGGGTGCGA GTCTTTGACA GAGAGATTGC TCAGATGTTC
    ATAAATATCA CCAAGACTGC AAAGGAGGCA CAGGTAGAGT CTGTAAGCAA GAAGGAGAAG
    GCCAAACAAA GGCCGCAAGC ATTAAACACT GTGGAAATGC TTCGTGTTGC TAGTTCTGCA
    CTGGGAATGG GTCCTCAACA TACAATGCAA ATTGCAGAGC GCCTGTACAC CCAAGGTTAC
    ATCAGCTATC CCCGAACAGA AACCACCCAC TATCCTGAAA ACTTTGACCT GAAAGGAACG
    CTTCGGCAGC AAGCTAACAA CCCTTTCTGG GCCGACATGG TTAAAACTTT GCTTGCTGAG
    GGAATCAACC GCCCTAGAAA AGGAACTGAT GCCGGAGACC ATCCACCAAT TACACCGATG
    CGTTCCGCTA CAGAGGCAGA ACTTGGTGGT GATGCTTGGA GACTGTATGA GTACATCACT
    CGGCACTTCA TTGCCACCAT CAGTCATGAC TGTAAATACC TTCAGACCAG TATTGCCTTC
    AGTGTGGGAC CTGAGAAGTT TACCACCACT TGCAAGGAGG TGATCTCACC AGGATTCACA
    GAAATTATGC CCTGGCAAGG TATTCCAAAG GAAGAGAGTT TCCAGCCGTG CGAAAGAGGA
    GACAAGTTCA GTGTGGATGA GATGAAACTG ATGGAAAAAC AGACATGCCC TCCAGACTAC
    TTGACTGAAG CTGAGCTCAT CTCTTTAATG GAGAGGCATG GCATTGGAAC TGATGCCAGC
    ATTCCAGTGC ACATTAATAA TATCTGCCAG CGCAATTATG TGACAGTGGA GAGTGGGCGC
    AGACTGAAGC CTACTAGCCT GGGCATTGTG TTGGTGCATG GATATTATAA GATAGATCCG
    GAACTGGTGC TTCCAACCAT TCGCAGTGCA GTGGAGAAGC AGCTCAACTT GATCGCTCAG
    GGGAAGGCAA ATTTCCAGCA AGTCCTGGAT CACACCTTGG ATATCTTTAA GAGGAAATTC
    CACTACTTTG TGGATTCAAT AGCAGGGATG GATGAGTTGA TGGAAGTATC ATTCTCACCT
    CTGGCTGCAA CTGGCAAACC ACTGTCTCGC TGTGGAAAAT GTCATCGATT TATGAAATAT
    ATTCAGGCCA AGCCTAGCCG CCTCCACTGC TCTCACTGTG ATGAAACCTA TAGTCTTCCT
    CAAAATGGCA CAATCAAACT GTACAAAGAA CTGCGCTGCC CACTAGATGA TTTTGAACTG
    GTTCTGTGGT CATCAGGGTC CCGAGGGAAA AGCTACCCTC TGTGTCCATA CTGCTACAAT
    CATGCCCCTT TTAGGGACAT GAAAAAAGGG ATGGGCTGCA ATGAGTGCAC ACATCCAACC
    TGCCAGCACT CACTTAGCAT GCTGGGAATT GCTCAGTGTG TTGAGTGTGA AAGTGGGGTA
    ATGGTCCTTG ACTCCACCTC TGGTCCAAAA TGGAAGATGT CCTGCAATAA ATGCAATGTC
    ATTGTGCACT TCTTTGAGAA TGCACATCGT GTGCGTGTAT CTGCAGAGAC TTGTGATGCA
    TGCGATGCTG CAGTGGTCCA TGTCGACTTC AACAAGGCAA AGTGTCCTCT ACCTGCAGAT
    CAAACACAGC ACGCTGGCTG TGTGTTCTGT GACCCTGTAT TTCAGGACCT AGTTGAGCTA
    AAACATGCCA CAATGAAACA TCCCATGCAC AAGGGTGGGC AGGGAAGAAG GCAAGGTAGA
    GGAAGAGGCA GGGGAAGAAG GGCAAACCCC AAAAAACCCA AGGACAAAAT GGCAGCCCTA
    GCTGCCTACT TTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa01723
    Clone ID Related Accession (Same CDS sequence) NM_001195572.2
    Accession Version NM_001195572.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-beta-1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CX914590.1, BX722431.1, CX926002.1, BC161096.1, EL862684.1, DT436561.1, DN070456.2 and DT436560.1. On Aug 9, 2013 this sequence version replaced NM_001195572.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGACAG TACTCATGGT TGCAGAGAAG CCTTCATTGG CACAGTCAAT CGCAAAGATT 
    CTTTCTAGAG GGAGTGCCTC CTCTCGTAAA GGACTGAATG GTGCGTGCTC TGTTCATGAA
    TACACAGGAT CATTCATGGG ACAGACTGTG CGCTTCAAGA TGACATCTGT CTGTGGTCAC
    GTTATGAGCT TGGACTTTAT AGGGAAATAC AACAACTGGG ACAAAGTGGA TCCAGCAGAG
    CTCTTTAGCA AAGCACCTAC AGAGAAGAAA GAAGCCAACC CAAAACTCAG TATGGTCAAA
    TTCCTTCAGG TGGAAGGACG TGGTTGTGAT TATGTTGTTC TTTGGCTGGA TTGTGATAAA
    GAAGGAGAAA ATATTTGCTT TGAGGTTCTA GATGCTATAC ATCCTGTAAT GAACAGAGCA
    CATGGCGGGG AGCGCACAGT ATACCGGGCA AAGTTCAGCT CCATTACTGA TACAGACATC
    CTAAATGCAA TGAACCGACT TGGGGAGCCC AATAAAAATG AAGCACTGTC AGTCGATGCC
    AGGCAAGAGC TGGACCTGAG AATTGGTTGT GCCTTTACAA GGTTTCAGAC AAAGTATTTT
    CAAGGAAAAT ACGGCAACCT AGACAGCTCT CTGATCTCCT TTGGACCGTG TCAGACACCA
    ACACTTGGAT TTTGTGTGGA AAGACATGAT AAGATCCAGT CTTTCAAACC AGAGACTTAT
    TGGGTCCTTC AAGTAAAGGT GAGCCGTGGA CAGGAAAACC CACTGATACT AGATTGGGAT
    CGGGTGCGAG TCTTTGACAG AGAGATTGCT CAGATGTTCA TAAATATCAC CAAGACTGCA
    AAGGAGGCAC AGGTAGAGTC TGTAAGCAAG AAGGAGAAGG CCAAACAAAG GCCGCAAGCA
    TTAAACACTG TGGAAATGCT TCGTGTTGCT AGTTCTGCAC TGGGAATGGG TCCTCAACAT
    ACAATGCAAA TTGCAGAGCG CCTGTACACC CAAGGTTACA TCAGCTATCC CCGAACAGAA
    ACCACCCACT ATCCTGAAAA CTTTGACCTG AAAGGAACGC TTCGGCAGCA AGCTAACAAC
    CCTTTCTGGG CCGACATGGT TAAAACTTTG CTTGCTGAGG GAATCAACCG CCCTAGAAAA
    GGAACTGATG CCGGAGACCA TCCACCAATT ACACCGATGC GTTCCGCTAC AGAGGCAGAA
    CTTGGTGGTG ATGCTTGGAG ACTGTATGAG TACATCACTC GGCACTTCAT TGCCACCATC
    AGTCATGACT GTAAATACCT TCAGACCAGT ATTGCCTTCA GTGTGGGACC TGAGAAGTTT
    ACCACCACTT GCAAGGAGGT GATCTCACCA GGATTCACAG AAATTATGCC CTGGCAAGGT
    ATTCCAAAGG AAGAGAGTTT CCAGCCGTGC GAAAGAGGAG ACAAGTTCAG TGTGGATGAG
    ATGAAACTGA TGGAAAAACA GACATGCCCT CCAGACTACT TGACTGAAGC TGAGCTCATC
    TCTTTAATGG AGAGGCATGG CATTGGAACT GATGCCAGCA TTCCAGTGCA CATTAATAAT
    ATCTGCCAGC GCAATTATGT GACAGTGGAG AGTGGGCGCA GACTGAAGCC TACTAGCCTG
    GGCATTGTGT TGGTGCATGG ATATTATAAG ATAGATCCGG AACTGGTGCT TCCAACCATT
    CGCAGTGCAG TGGAGAAGCA GCTCAACTTG ATCGCTCAGG GGAAGGCAAA TTTCCAGCAA
    GTCCTGGATC ACACCTTGGA TATCTTTAAG AGGAAATTCC ACTACTTTGT GGATTCAATA
    GCAGGGATGG ATGAGTTGAT GGAAGTATCA TTCTCACCTC TGGCTGCAAC TGGCAAACCA
    CTGTCTCGCT GTGGAAAATG TCATCGATTT ATGAAATATA TTCAGGCCAA GCCTAGCCGC
    CTCCACTGCT CTCACTGTGA TGAAACCTAT AGTCTTCCTC AAAATGGCAC AATCAAACTG
    TACAAAGAAC TGCGCTGCCC ACTAGATGAT TTTGAACTGG TTCTGTGGTC ATCAGGGTCC
    CGAGGGAAAA GCTACCCTCT GTGTCCATAC TGCTACAATC ATGCCCCTTT TAGGGACATG
    AAAAAAGGGA TGGGCTGCAA TGAGTGCACA CATCCAACCT GCCAGCACTC ACTTAGCATG
    CTGGGAATTG CTCAGTGTGT TGAGTGTGAA AGTGGGGTAA TGGTCCTTGA CTCCACCTCT
    GGTCCAAAAT GGAAGATGTC CTGCAATAAA TGCAATGTCA TTGTGCACTT CTTTGAGAAT
    GCACATCGTG TGCGTGTATC TGCAGAGACT TGTGATGCAT GCGATGCTGC AGTGGTCCAT
    GTCGACTTCA ACAAGGCAAA GTGTCCTCTA CCTGCAGATC AAACACAGCA CGCTGGCTGT
    GTGTTCTGTG ACCCTGTATT TCAGGACCTA GTTGAGCTAA AACATGCCAC AATGAAACAT
    CCCATGCACA AGGGTGGGCA GGGAAGAAGG CAAGGTAGAG GAAGAGGCAG GGGAAGAAGG
    GCAAACCCCA AAAAACCCAA GGACAAAATG GCAGCCCTAG CTGCCTACTT TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001182501.1
    CDS196..2772
    Translation

    Target ORF information:

    RefSeq Version NM_001195572.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001195572.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAAGACAG TACTCATGGT TGCAGAGAAG CCTTCATTGG CACAGTCAAT CGCAAAGATT 
    CTTTCTAGAG GGAGTGCCTC CTCTCGTAAA GGACTGAATG GTGCGTGCTC TGTTCATGAA
    TACACAGGAT CATTCATGGG ACAGACTGTG CGCTTCAAGA TGACATCTGT CTGTGGTCAC
    GTTATGAGCT TGGACTTTAT AGGGAAATAC AACAACTGGG ACAAAGTGGA TCCAGCAGAG
    CTCTTTAGCA AAGCACCTAC AGAGAAGAAA GAAGCCAACC CAAAACTCAG TATGGTCAAA
    TTCCTTCAGG TGGAAGGACG TGGTTGTGAT TATGTTGTTC TTTGGCTGGA TTGTGATAAA
    GAAGGAGAAA ATATTTGCTT TGAGGTTCTA GATGCTATAC ATCCTGTAAT GAACAGAGCA
    CATGGCGGGG AGCGCACAGT ATACCGGGCA AAGTTCAGCT CCATTACTGA TACAGACATC
    CTAAATGCAA TGAACCGACT TGGGGAGCCC AATAAAAATG AAGCACTGTC AGTCGATGCC
    AGGCAAGAGC TGGACCTGAG AATTGGTTGT GCCTTTACAA GGTTTCAGAC AAAGTATTTT
    CAAGGAAAAT ACGGCAACCT AGACAGCTCT CTGATCTCCT TTGGACCGTG TCAGACACCA
    ACACTTGGAT TTTGTGTGGA AAGACATGAT AAGATCCAGT CTTTCAAACC AGAGACTTAT
    TGGGTCCTTC AAGTAAAGGT GAGCCGTGGA CAGGAAAACC CACTGATACT AGATTGGGAT
    CGGGTGCGAG TCTTTGACAG AGAGATTGCT CAGATGTTCA TAAATATCAC CAAGACTGCA
    AAGGAGGCAC AGGTAGAGTC TGTAAGCAAG AAGGAGAAGG CCAAACAAAG GCCGCAAGCA
    TTAAACACTG TGGAAATGCT TCGTGTTGCT AGTTCTGCAC TGGGAATGGG TCCTCAACAT
    ACAATGCAAA TTGCAGAGCG CCTGTACACC CAAGGTTACA TCAGCTATCC CCGAACAGAA
    ACCACCCACT ATCCTGAAAA CTTTGACCTG AAAGGAACGC TTCGGCAGCA AGCTAACAAC
    CCTTTCTGGG CCGACATGGT TAAAACTTTG CTTGCTGAGG GAATCAACCG CCCTAGAAAA
    GGAACTGATG CCGGAGACCA TCCACCAATT ACACCGATGC GTTCCGCTAC AGAGGCAGAA
    CTTGGTGGTG ATGCTTGGAG ACTGTATGAG TACATCACTC GGCACTTCAT TGCCACCATC
    AGTCATGACT GTAAATACCT TCAGACCAGT ATTGCCTTCA GTGTGGGACC TGAGAAGTTT
    ACCACCACTT GCAAGGAGGT GATCTCACCA GGATTCACAG AAATTATGCC CTGGCAAGGT
    ATTCCAAAGG AAGAGAGTTT CCAGCCGTGC GAAAGAGGAG ACAAGTTCAG TGTGGATGAG
    ATGAAACTGA TGGAAAAACA GACATGCCCT CCAGACTACT TGACTGAAGC TGAGCTCATC
    TCTTTAATGG AGAGGCATGG CATTGGAACT GATGCCAGCA TTCCAGTGCA CATTAATAAT
    ATCTGCCAGC GCAATTATGT GACAGTGGAG AGTGGGCGCA GACTGAAGCC TACTAGCCTG
    GGCATTGTGT TGGTGCATGG ATATTATAAG ATAGATCCGG AACTGGTGCT TCCAACCATT
    CGCAGTGCAG TGGAGAAGCA GCTCAACTTG ATCGCTCAGG GGAAGGCAAA TTTCCAGCAA
    GTCCTGGATC ACACCTTGGA TATCTTTAAG AGGAAATTCC ACTACTTTGT GGATTCAATA
    GCAGGGATGG ATGAGTTGAT GGAAGTATCA TTCTCACCTC TGGCTGCAAC TGGCAAACCA
    CTGTCTCGCT GTGGAAAATG TCATCGATTT ATGAAATATA TTCAGGCCAA GCCTAGCCGC
    CTCCACTGCT CTCACTGTGA TGAAACCTAT AGTCTTCCTC AAAATGGCAC AATCAAACTG
    TACAAAGAAC TGCGCTGCCC ACTAGATGAT TTTGAACTGG TTCTGTGGTC ATCAGGGTCC
    CGAGGGAAAA GCTACCCTCT GTGTCCATAC TGCTACAATC ATGCCCCTTT TAGGGACATG
    AAAAAAGGGA TGGGCTGCAA TGAGTGCACA CATCCAACCT GCCAGCACTC ACTTAGCATG
    CTGGGAATTG CTCAGTGTGT TGAGTGTGAA AGTGGGGTAA TGGTCCTTGA CTCCACCTCT
    GGTCCAAAAT GGAAGATGTC CTGCAATAAA TGCAATGTCA TTGTGCACTT CTTTGAGAAT
    GCACATCGTG TGCGTGTATC TGCAGAGACT TGTGATGCAT GCGATGCTGC AGTGGTCCAT
    GTCGACTTCA ACAAGGCAAA GTGTCCTCTA CCTGCAGATC AAACACAGCA CGCTGGCTGT
    GTGTTCTGTG ACCCTGTATT TCAGGACCTA GTTGAGCTAA AACATGCCAC AATGAAACAT
    CCCATGCACA AGGGTGGGCA GGGAAGAAGG CAAGGTAGAG GAAGAGGCAG GGGAAGAAGG
    GCAAACCCCA AAAAACCCAA GGACAAAATG GCAGCCCTAG CTGCCTACTT TGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31826
    Clone ID Related Accession (Same CDS sequence) XM_012956162.2
    Accession Version XM_012956162.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956162.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAGAG CACATGGCGG GGAGCGCACA GTATACCGGG CAAAGTTCAG CTCCATTACT 
    GATACAGACA TCCTGAATGC AATGAACCGA CTTGGGGAGC CCAATAAAAA TGAAGCACTG
    TCAGTCGATG CCAGGCAAGA GCTGGACCTG AGAATTGGTT GTGCCTTTAC AAGGTTTCAG
    ACAAAGTATT TTCAAGGAAA ATATGGCAAC CTAGACAGCT CTCTGATCTC CTTTGGACCG
    TGTCAGACAC CAACACTTGG ATTTTGTGTG GAAAGACATG ATAAGATCCA GTCTTTCAAA
    CCAGAGACTT ATTGGGTCCT TCAAGTAAAG GTGAGCCGTG GACAGGAAAA CCCACTGATA
    CTAGATTGGG ATCGGGTGCG AGTCTTTGAC AGAGAGATTG CTCAGATGTT CATAAATATC
    ACCAAGACTG CAAAGGAGGC ACAGGTAGAG TCTGTAAGCA AGAAGGAGAA GGCCAAACAA
    AGGCCGCAAG CATTAAACAC TGTGGAAATG CTTCGTGTTG CTAGTTCTGC ACTGGGAATG
    GGTCCTCAAC ATACAATGCA AATTGCAGAG CGCCTGTACA CCCAAGGTTA CATCAGCTAT
    CCCCGAACAG AAACCACCCA CTATCCTGAA AACTTTGACC TGAAAGGAAC GCTTCGGCAG
    CAAGCTAACA ACCCTTTCTG GGCCGACATG GTTAAAACTT TGCTTGCTGA GGGAATCAAC
    CGCCCTAGAA AAGGAACTGA TGCCGGAGAC CATCCACCAA TTACACCGAT GCGTTCAGCT
    ACAGAGGCAG AACTTGGTGG TGATGCTTGG AGACTGTATG AGTACATCAC TCGGCACTTC
    ATTGCCACCA TCAGTCATGA CTGTAAATAC CTTCAGACCA GTATTGCCTT CAGTGTGGGA
    CCTGAGAAGT TTACCACCAC TTGCAAGGAG GTGATCTCAC CAGGATTCAC AGAAATTATG
    CCCTGGCAAG GTATTCCAAA GGAAGAGAGT TTCCAGCCGT GCGAAAGAGG AGACAAGTTC
    AGTGTGGATG AGATGAAACT GATGGAAAAA CAGACATGCC CTCCAGACTA CTTGACTGAA
    GCTGAGCTCA TCTCTTTAAT GGAGAGGCAT GGCATTGGAA CTGATGCCAG CATTCCAGTG
    CACATTAATA ATATCTGCCA GCGCAATTAT GTGACAGTGG AGAGTGGGCG CAGACTGAAG
    CCTACTAGCC TGGGCATTGT GTTGGTGCAT GGATATTATA AGATAGATCC GGAACTGGTG
    CTTCCAACCA TTCGCAGTGC AGTGGAGAAG CAGCTCAACT TGATCGCTCA GGGGAAGGCA
    AATTTCCAGC AAGTCCTGGA TCACACCTTG GATATCTTTA AGAGGAAATT CCACTACTTT
    GTGGATTCAA TAGCAGGGAT GGATGAGTTG ATGGAAGTAT CATTCTCACC TCTTGCTGCA
    ACTGGCAAAC CACTGTCTCG CTGTGGAAAA TGTCATCGAT TTATGAAATA TATTCAGGCC
    AAGCCTAGCC GCCTCCACTG CTCTCACTGT GATGAAACCT ATAGTCTTCC TCAAAATGGC
    ACAATCAAAC TGTACAAAGA ACTGCGCTGC CCACTAGATG ATTTTGAACT GGTTCTGTGG
    TCATCAGGGT CCCGAGGGAA AAGCTACCCT CTGTGTCCAT ACTGCTACAA TCATGCCCCT
    TTTAGGGACA TGAAAAAAGG GATGGGCTGC AATGAGTGCA CACATCCAAC CTGCCAGCAC
    TCACTTAGCA TGCTGGGAAT TGCTCAGTGT GTTGAGTGTG AAAGTGGGGT AATGGTCCTT
    GACTCCACCT CTGGTCCAAA ATGGAAGATG TCCTGCAATA AATGCAATGT CATTGTGCAC
    TTCTTTGAGA ATGCACATCG TGTGCGTGTA TCTGCAGAGA CTTGTGATGC ATGCGATGCT
    GCAGTGGTCC ATGTCGACTT CAACAAGGCA AAGTGTCCTC TACCTGCAGA TCAAACACAG
    CACGCTGGCT GTGTGTTCTG TGACCCTGTA TTTCAGGACC TAGTTGAGCT AAAACATGCC
    ACAATGAAAC ATCCCATGCA CAAGGGTGGG CAGGGAAGAA GGCAAGGTAG AGGAAGAGGC
    AGGGGAAGAA GGGCAAACCC CAAAAAACCC AAGGACAAAA TGGCAGCCCT AGCTGCCTAC
    TTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811616.1
    CDS187..2355
    Translation

    Target ORF information:

    RefSeq Version XM_012956162.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) III beta (top3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956162.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAGAG CACATGGCGG GGAGCGCACA GTATACCGGG CAAAGTTCAG CTCCATTACT 
    GATACAGACA TCCTGAATGC AATGAACCGA CTTGGGGAGC CCAATAAAAA TGAAGCACTG
    TCAGTCGATG CCAGGCAAGA GCTGGACCTG AGAATTGGTT GTGCCTTTAC AAGGTTTCAG
    ACAAAGTATT TTCAAGGAAA ATATGGCAAC CTAGACAGCT CTCTGATCTC CTTTGGACCG
    TGTCAGACAC CAACACTTGG ATTTTGTGTG GAAAGACATG ATAAGATCCA GTCTTTCAAA
    CCAGAGACTT ATTGGGTCCT TCAAGTAAAG GTGAGCCGTG GACAGGAAAA CCCACTGATA
    CTAGATTGGG ATCGGGTGCG AGTCTTTGAC AGAGAGATTG CTCAGATGTT CATAAATATC
    ACCAAGACTG CAAAGGAGGC ACAGGTAGAG TCTGTAAGCA AGAAGGAGAA GGCCAAACAA
    AGGCCGCAAG CATTAAACAC TGTGGAAATG CTTCGTGTTG CTAGTTCTGC ACTGGGAATG
    GGTCCTCAAC ATACAATGCA AATTGCAGAG CGCCTGTACA CCCAAGGTTA CATCAGCTAT
    CCCCGAACAG AAACCACCCA CTATCCTGAA AACTTTGACC TGAAAGGAAC GCTTCGGCAG
    CAAGCTAACA ACCCTTTCTG GGCCGACATG GTTAAAACTT TGCTTGCTGA GGGAATCAAC
    CGCCCTAGAA AAGGAACTGA TGCCGGAGAC CATCCACCAA TTACACCGAT GCGTTCAGCT
    ACAGAGGCAG AACTTGGTGG TGATGCTTGG AGACTGTATG AGTACATCAC TCGGCACTTC
    ATTGCCACCA TCAGTCATGA CTGTAAATAC CTTCAGACCA GTATTGCCTT CAGTGTGGGA
    CCTGAGAAGT TTACCACCAC TTGCAAGGAG GTGATCTCAC CAGGATTCAC AGAAATTATG
    CCCTGGCAAG GTATTCCAAA GGAAGAGAGT TTCCAGCCGT GCGAAAGAGG AGACAAGTTC
    AGTGTGGATG AGATGAAACT GATGGAAAAA CAGACATGCC CTCCAGACTA CTTGACTGAA
    GCTGAGCTCA TCTCTTTAAT GGAGAGGCAT GGCATTGGAA CTGATGCCAG CATTCCAGTG
    CACATTAATA ATATCTGCCA GCGCAATTAT GTGACAGTGG AGAGTGGGCG CAGACTGAAG
    CCTACTAGCC TGGGCATTGT GTTGGTGCAT GGATATTATA AGATAGATCC GGAACTGGTG
    CTTCCAACCA TTCGCAGTGC AGTGGAGAAG CAGCTCAACT TGATCGCTCA GGGGAAGGCA
    AATTTCCAGC AAGTCCTGGA TCACACCTTG GATATCTTTA AGAGGAAATT CCACTACTTT
    GTGGATTCAA TAGCAGGGAT GGATGAGTTG ATGGAAGTAT CATTCTCACC TCTTGCTGCA
    ACTGGCAAAC CACTGTCTCG CTGTGGAAAA TGTCATCGAT TTATGAAATA TATTCAGGCC
    AAGCCTAGCC GCCTCCACTG CTCTCACTGT GATGAAACCT ATAGTCTTCC TCAAAATGGC
    ACAATCAAAC TGTACAAAGA ACTGCGCTGC CCACTAGATG ATTTTGAACT GGTTCTGTGG
    TCATCAGGGT CCCGAGGGAA AAGCTACCCT CTGTGTCCAT ACTGCTACAA TCATGCCCCT
    TTTAGGGACA TGAAAAAAGG GATGGGCTGC AATGAGTGCA CACATCCAAC CTGCCAGCAC
    TCACTTAGCA TGCTGGGAAT TGCTCAGTGT GTTGAGTGTG AAAGTGGGGT AATGGTCCTT
    GACTCCACCT CTGGTCCAAA ATGGAAGATG TCCTGCAATA AATGCAATGT CATTGTGCAC
    TTCTTTGAGA ATGCACATCG TGTGCGTGTA TCTGCAGAGA CTTGTGATGC ATGCGATGCT
    GCAGTGGTCC ATGTCGACTT CAACAAGGCA AAGTGTCCTC TACCTGCAGA TCAAACACAG
    CACGCTGGCT GTGTGTTCTG TGACCCTGTA TTTCAGGACC TAGTTGAGCT AAAACATGCC
    ACAATGAAAC ATCCCATGCA CAAGGGTGGG CAGGGAAGAA GGCAAGGTAG AGGAAGAGGC
    AGGGGAAGAA GGGCAAACCC CAAAAAACCC AAGGACAAAA TGGCAGCCCT AGCTGCCTAC
    TTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa47578
    Clone ID Related Accession (Same CDS sequence) XM_012956162.3
    Accession Version XM_012956162.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2169bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 3-beta-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956162.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAGAG CACATGGCGG GGAGCGCACA GTATACCGGG CAAAGTTCAG CTCCATTACT 
    GATACAGACA TCCTAAATGC AATGAACCGA CTTGGGGAGC CCAATAAAAA TGAAGCACTG
    TCAGTCGATG CCAGGCAAGA GCTGGACCTG AGAATTGGTT GTGCCTTTAC AAGGTTTCAG
    ACAAAGTATT TTCAAGGAAA ATACGGCAAC CTAGACAGCT CTCTGATCTC CTTTGGACCG
    TGTCAGACAC CAACACTTGG ATTTTGTGTG GAAAGACATG ATAAGATCCA GTCTTTCAAA
    CCAGAGACTT ATTGGGTCCT TCAAGTAAAG GTGAGCCGTG GACAGGAAAA CCCACTGATA
    CTAGATTGGG ATCGGGTGCG AGTCTTTGAC AGAGAGATTG CTCAGATGTT CATAAATATC
    ACCAAGACTG CAAAGGAGGC ACAGGTAGAG TCTGTAAGCA AGAAGGAGAA GGCCAAACAA
    AGGCCGCAAG CATTAAACAC TGTGGAAATG CTTCGTGTTG CTAGTTCTGC ACTGGGAATG
    GGTCCTCAAC ATACAATGCA AATTGCAGAG CGCCTGTACA CCCAAGGTTA CATCAGCTAT
    CCCCGAACAG AAACCACCCA CTATCCTGAA AACTTTGACC TGAAAGGAAC GCTTCGGCAG
    CAAGCTAACA ACCCTTTCTG GGCCGACATG GTTAAAACTT TGCTTGCTGA GGGAATCAAC
    CGCCCTAGAA AAGGAACTGA TGCCGGAGAC CATCCACCAA TTACACCGAT GCGTTCAGCT
    ACAGAGGCAG AACTTGGTGG TGATGCTTGG AGACTGTATG AGTACATCAC TCGGCACTTC
    ATTGCCACCA TCAGTCATGA CTGTAAATAC CTTCAGACCA GTATTGCCTT CAGTGTGGGA
    CCTGAGAAGT TTACCACCAC TTGCAAGGAG GTGATCTCAC CAGGATTCAC AGAAATTATG
    CCCTGGCAAG GTATTCCAAA GGAAGAGAGT TTCCAGCCGT GCGAAAGAGG AGACAAGTTC
    AGTGTGGATG AGATGAAACT GATGGAAAAA CAGACATGCC CTCCAGACTA CTTGACTGAA
    GCTGAGCTCA TCTCTTTAAT GGAGAGGCAT GGCATTGGAA CTGATGCCAG CATTCCAGTG
    CACATTAATA ATATCTGCCA GCGCAATTAT GTGACAGTGG AGAGTGGGCG CAGACTGAAG
    CCTACTAGCC TGGGCATTGT GTTGGTGCAT GGATATTATA AGATAGATCC GGAACTGGTG
    CTTCCAACCA TTCGCAGTGC AGTGGAGAAG CAGCTCAACT TGATCGCTCA GGGGAAGGCA
    AATTTCCAGC AAGTCCTGGA TCACACCTTG GATATCTTTA AGAGGAAATT CCACTACTTT
    GTGGATTCAA TAGCAGGGAT GGATGAGTTG ATGGAAGTAT CATTCTCACC TCTGGCTGCA
    ACTGGCAAAC CACTGTCTCG CTGTGGAAAA TGTCATCGAT TTATGAAATA TATTCAGGCC
    AAGCCTAGCC GCCTCCACTG CTCTCACTGT GATGAAACCT ATAGTCTTCC TCAAAATGGC
    ACAATCAAAC TGTACAAAGA ACTGCGCTGC CCACTAGATG ATTTTGAACT GGTTCTGTGG
    TCATCAGGGT CCCGAGGGAA AAGCTACCCT CTGTGTCCAT ACTGCTACAA TCATGCCCCT
    TTTAGGGACA TGAAAAAAGG GATGGGCTGC AATGAGTGCA CACATCCAAC CTGCCAGCAC
    TCACTTAGCA TGCTGGGAAT TGCTCAGTGT GTTGAGTGTG AAAGTGGGGT AATGGTCCTT
    GACTCCACCT CTGGTCCAAA ATGGAAGATG TCCTGCAATA AATGCAATGT CATTGTGCAC
    TTCTTTGAGA ATGCACATCG TGTGCGTGTA TCTGCAGAGA CTTGTGATGC ATGCGATGCT
    GCAGTGGTCC ATGTCGACTT CAACAAGGCA AAGTGTCCTC TACCTGCAGA TCAAACACAG
    CACGCTGGCT GTGTGTTCTG TGACCCTGTA TTTCAGGACC TAGTTGAGCT AAAACATGCC
    ACAATGAAAC ATCCCATGCA CAAGGGTGGG CAGGGAAGAA GGCAAGGTAG AGGAAGAGGC
    AGGGGAAGAA GGGCAAACCC CAAAAAACCC AAGGACAAAA TGGCAGCCCT AGCTGCCTAC
    TTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012811616.1
    CDS188..2356
    Translation

    Target ORF information:

    RefSeq Version XM_012956162.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase III beta (top3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012956162.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAGAG CACATGGCGG GGAGCGCACA GTATACCGGG CAAAGTTCAG CTCCATTACT 
    GATACAGACA TCCTAAATGC AATGAACCGA CTTGGGGAGC CCAATAAAAA TGAAGCACTG
    TCAGTCGATG CCAGGCAAGA GCTGGACCTG AGAATTGGTT GTGCCTTTAC AAGGTTTCAG
    ACAAAGTATT TTCAAGGAAA ATACGGCAAC CTAGACAGCT CTCTGATCTC CTTTGGACCG
    TGTCAGACAC CAACACTTGG ATTTTGTGTG GAAAGACATG ATAAGATCCA GTCTTTCAAA
    CCAGAGACTT ATTGGGTCCT TCAAGTAAAG GTGAGCCGTG GACAGGAAAA CCCACTGATA
    CTAGATTGGG ATCGGGTGCG AGTCTTTGAC AGAGAGATTG CTCAGATGTT CATAAATATC
    ACCAAGACTG CAAAGGAGGC ACAGGTAGAG TCTGTAAGCA AGAAGGAGAA GGCCAAACAA
    AGGCCGCAAG CATTAAACAC TGTGGAAATG CTTCGTGTTG CTAGTTCTGC ACTGGGAATG
    GGTCCTCAAC ATACAATGCA AATTGCAGAG CGCCTGTACA CCCAAGGTTA CATCAGCTAT
    CCCCGAACAG AAACCACCCA CTATCCTGAA AACTTTGACC TGAAAGGAAC GCTTCGGCAG
    CAAGCTAACA ACCCTTTCTG GGCCGACATG GTTAAAACTT TGCTTGCTGA GGGAATCAAC
    CGCCCTAGAA AAGGAACTGA TGCCGGAGAC CATCCACCAA TTACACCGAT GCGTTCAGCT
    ACAGAGGCAG AACTTGGTGG TGATGCTTGG AGACTGTATG AGTACATCAC TCGGCACTTC
    ATTGCCACCA TCAGTCATGA CTGTAAATAC CTTCAGACCA GTATTGCCTT CAGTGTGGGA
    CCTGAGAAGT TTACCACCAC TTGCAAGGAG GTGATCTCAC CAGGATTCAC AGAAATTATG
    CCCTGGCAAG GTATTCCAAA GGAAGAGAGT TTCCAGCCGT GCGAAAGAGG AGACAAGTTC
    AGTGTGGATG AGATGAAACT GATGGAAAAA CAGACATGCC CTCCAGACTA CTTGACTGAA
    GCTGAGCTCA TCTCTTTAAT GGAGAGGCAT GGCATTGGAA CTGATGCCAG CATTCCAGTG
    CACATTAATA ATATCTGCCA GCGCAATTAT GTGACAGTGG AGAGTGGGCG CAGACTGAAG
    CCTACTAGCC TGGGCATTGT GTTGGTGCAT GGATATTATA AGATAGATCC GGAACTGGTG
    CTTCCAACCA TTCGCAGTGC AGTGGAGAAG CAGCTCAACT TGATCGCTCA GGGGAAGGCA
    AATTTCCAGC AAGTCCTGGA TCACACCTTG GATATCTTTA AGAGGAAATT CCACTACTTT
    GTGGATTCAA TAGCAGGGAT GGATGAGTTG ATGGAAGTAT CATTCTCACC TCTGGCTGCA
    ACTGGCAAAC CACTGTCTCG CTGTGGAAAA TGTCATCGAT TTATGAAATA TATTCAGGCC
    AAGCCTAGCC GCCTCCACTG CTCTCACTGT GATGAAACCT ATAGTCTTCC TCAAAATGGC
    ACAATCAAAC TGTACAAAGA ACTGCGCTGC CCACTAGATG ATTTTGAACT GGTTCTGTGG
    TCATCAGGGT CCCGAGGGAA AAGCTACCCT CTGTGTCCAT ACTGCTACAA TCATGCCCCT
    TTTAGGGACA TGAAAAAAGG GATGGGCTGC AATGAGTGCA CACATCCAAC CTGCCAGCAC
    TCACTTAGCA TGCTGGGAAT TGCTCAGTGT GTTGAGTGTG AAAGTGGGGT AATGGTCCTT
    GACTCCACCT CTGGTCCAAA ATGGAAGATG TCCTGCAATA AATGCAATGT CATTGTGCAC
    TTCTTTGAGA ATGCACATCG TGTGCGTGTA TCTGCAGAGA CTTGTGATGC ATGCGATGCT
    GCAGTGGTCC ATGTCGACTT CAACAAGGCA AAGTGTCCTC TACCTGCAGA TCAAACACAG
    CACGCTGGCT GTGTGTTCTG TGACCCTGTA TTTCAGGACC TAGTTGAGCT AAAACATGCC
    ACAATGAAAC ATCCCATGCA CAAGGGTGGG CAGGGAAGAA GGCAAGGTAG AGGAAGAGGC
    AGGGGAAGAA GGGCAAACCC CAAAAAACCC AAGGACAAAA TGGCAGCCCT AGCTGCCTAC
    TTTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.