mical2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mical2a
Entrez Gene ID	569607
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 2a
Synonyms	mical2
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 2a Names protein MICAL-2 F-actin-methionine sulfoxide oxidase MICAL2 microtubule associated monoxygenase, calponin and LIM domain containing 2 microtubule associated monoxygenase, calponin and LIM domain containing 2a molecule interacting with CasL 2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021478017.1	XP_021333692.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X1
XM_021478021.1	XP_021333696.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X6
XM_021478018.1	XP_021333693.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X2
XM_009303577.3	XP_009301852.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X3
XM_021478020.1	XP_021333695.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X5
XM_009303577.2	XP_009301852.1	protein-methionine sulfoxide oxidase MICAL2
XM_021478019.1	XP_021333694.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X4
XM_021478022.1	XP_021333697.1	F-actin-methionine sulfoxide oxidase MICAL2 isoform X7

Pathways from BioSystems
Gene Ontology

The following mical2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mical2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45816	XM_021478021.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa45817	XM_021478022.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa143555	XM_021478020.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa45812	XM_021478017.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45813	XM_021478018.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa45814	XM_009303577.3 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa45815	XM_021478019.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa45814	XM_009303577.2	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mical2a ( XM_021478021.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333696.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa45816
Clone ID Related Accession (Same CDS sequence)	XM_021478021.1
Accession Version	XM_021478021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGC GCACAGTGGG CAAAGTGTCT 
CTGAGAGTGG GTGCTAGAGC CGACGCTCTG GTTACCCTGT ATGAAAATGA TCATAGACCT 
CAGACAAGCA GCTCTGGCAC TCCGCCTTCC ATAAAGAGAG AGTTTCCTCA GACTCTGGGC 
GATGTGTGTC ATGCCTGTAA GAAGCGTGTG TTTGTGGTGG AGAGACTCAG CGCAGAGGGA 
TTTCACTTTC ACCGGGAATG TTTCCGCTGT GAGACCTGCA GAGCTCCGCT ACGTCAGGGA 
GGACACTGCT TCCATTCTGA GGAAGGAAAG TTGTACTGCA AACTCCACTT TGCTCAAAGG 
AAATCCTTAG TGAAAAATGG AAGAACAGAA ACTCTACATA CGACGTGTAA TGACGGCTCT 
CACCATGGCT CAAGCTGGAC TCCAGAGAGC CATGAGGATC ATCTCCAGGC TGAGTCCGCA 
GGTATCTTGT CCTGTGTGAA GAGAAGCCTG TGCTGGCCGC TGCACGTCAG CCAGACTGTG 
TGTGCTGTGC CGCGTCGTGT GTGTACATGG CTGCATGGTA AAGTGTCGGC CGCTCGAGTC 
CACCTGATGG ACAACGCTGG CGATTACGCC TTCCTGTACG AGCTGCTCAG CGTGGGTCTG 
CCTCTGCTGG CCATTCTCCA TGAACAGCTG CTGCAAATGT ATGCCGAGGC TGAACCGTTC 
AATCTCCAGC CTCTACAGCA CTGGCTGGAG GAGCACATGG GCCACTGGGT CTTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333696.1
CDS	274..3213
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478021.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478021.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGC GCACAGTGGG CAAAGTGTCT 
CTGAGAGTGG GTGCTAGAGC CGACGCTCTG GTTACCCTGT ATGAAAATGA TCATAGACCT 
CAGACAAGCA GCTCTGGCAC TCCGCCTTCC ATAAAGAGAG AGTTTCCTCA GACTCTGGGC 
GATGTGTGTC ATGCCTGTAA GAAGCGTGTG TTTGTGGTGG AGAGACTCAG CGCAGAGGGA 
TTTCACTTTC ACCGGGAATG TTTCCGCTGT GAGACCTGCA GAGCTCCGCT ACGTCAGGGA 
GGACACTGCT TCCATTCTGA GGAAGGAAAG TTGTACTGCA AACTCCACTT TGCTCAAAGG 
AAATCCTTAG TGAAAAATGG AAGAACAGAA ACTCTACATA CGACGTGTAA TGACGGCTCT 
CACCATGGCT CAAGCTGGAC TCCAGAGAGC CATGAGGATC ATCTCCAGGC TGAGTCCGCA 
GGTATCTTGT CCTGTGTGAA GAGAAGCCTG TGCTGGCCGC TGCACGTCAG CCAGACTGTG 
TGTGCTGTGC CGCGTCGTGT GTGTACATGG CTGCATGGTA AAGTGTCGGC CGCTCGAGTC 
CACCTGATGG ACAACGCTGG CGATTACGCC TTCCTGTACG AGCTGCTCAG CGTGGGTCTG 
CCTCTGCTGG CCATTCTCCA TGAACAGCTG CTGCAAATGT ATGCCGAGGC TGAACCGTTC 
AATCTCCAGC CTCTACAGCA CTGGCTGGAG GAGCACATGG GCCACTGGGT CTTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_021478022.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333697.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa45817
Clone ID Related Accession (Same CDS sequence)	XM_021478022.1
Accession Version	XM_021478022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGC CTTCCATAAA GAGAGAGTTT 
CCTCAGACTC TGGGCGATGT GTGTCATGCC TGTAAGAAGC GTGTGTTTGT GGTGGAGAGA 
CTCAGCGCAG AGGGATTTCA CTTTCACCGG GAATGTTTCC GCTGTGAGAC CTGCAGAGCT 
CCGCTACGTC AGGGAGGACA CTGCTTCCAT TCTGAGGAAG GAAAGTTGTA CTGCAAACTC 
CACTTTGCTC AAAGGAAATC CTTAGTGAAA AATGGAAGAA CAGAAACTCT ACATACGACG 
TGTAATGACG GCTCTCACCA TGGCTCAAGC TGGACTCCAG AGAGCCATGA GGATCATCTC 
CAGGCTGAGT CCGCAGGTAT CTTGTCCTGT GTGAAGAGAA GCCTGTGCTG GCCGCTGCAC 
GTCAGCCAGA CTGTGTGTGC TGTGCCGCGT CGTGTGTGTA CATGGCTGCA TGGTAAAGTG 
TCGGCCGCTC GAGTCCACCT GATGGACAAC GCTGGCGATT ACGCCTTCCT GTACGAGCTG 
CTCAGCGTGG GTCTGCCTCT GCTGGCCATT CTCCATGAAC AGCTGCTGCA AATGTATGCC 
GAGGCTGAAC CGTTCAATCT CCAGCCTCTA CAGCACTGGC TGGAGGAGCA CATGGGCCAC 
TGGGTCTTAG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333697.1
CDS	274..3108
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478022.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478022.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGC CTTCCATAAA GAGAGAGTTT 
CCTCAGACTC TGGGCGATGT GTGTCATGCC TGTAAGAAGC GTGTGTTTGT GGTGGAGAGA 
CTCAGCGCAG AGGGATTTCA CTTTCACCGG GAATGTTTCC GCTGTGAGAC CTGCAGAGCT 
CCGCTACGTC AGGGAGGACA CTGCTTCCAT TCTGAGGAAG GAAAGTTGTA CTGCAAACTC 
CACTTTGCTC AAAGGAAATC CTTAGTGAAA AATGGAAGAA CAGAAACTCT ACATACGACG 
TGTAATGACG GCTCTCACCA TGGCTCAAGC TGGACTCCAG AGAGCCATGA GGATCATCTC 
CAGGCTGAGT CCGCAGGTAT CTTGTCCTGT GTGAAGAGAA GCCTGTGCTG GCCGCTGCAC 
GTCAGCCAGA CTGTGTGTGC TGTGCCGCGT CGTGTGTGTA CATGGCTGCA TGGTAAAGTG 
TCGGCCGCTC GAGTCCACCT GATGGACAAC GCTGGCGATT ACGCCTTCCT GTACGAGCTG 
CTCAGCGTGG GTCTGCCTCT GCTGGCCATT CTCCATGAAC AGCTGCTGCA AATGTATGCC 
GAGGCTGAAC CGTTCAATCT CCAGCCTCTA CAGCACTGGC TGGAGGAGCA CATGGGCCAC 
TGGGTCTTAG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_021478020.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333695.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa143555
Clone ID Related Accession (Same CDS sequence)	XM_021478020.1
Accession Version	XM_021478020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGAGGAAAG CCCAGACTCA AAAGGCCAGG 
GAGTTTCACA AAAAAAGCAT AAAGGAGAAG GCTGTGCATT TGAGCGCTCT GTTCTCTGGC 
GAGGCCTCCG CTCCTGATCA GCGCACAGTG GGCAAAGTGT CTCTGAGAGT GGGTGCTAGA 
GCCGACGCTC TGGTTACCCT GTATGAAAAT GATCATAGAC CTCAGACAAG CAGCTCTGGC 
ACTCCGCCTT CCATAAAGAG AGAGTTTCCT CAGACTCTGG GCGATGTGTG TCATGCCTGT 
AAGAAGCGTG TGTTTGTGGT GGAGAGACTC AGCGCAGAGG GATTTCACTT TCACCGGGAA 
TGTTTCCGCT GTGAGACCTG CAGAGCTCCG CTACGTCAGG GAGGACACTG CTTCCATTCT 
GAGGAAGGAA AGTTGTACTG CAAACTCCAC TTTGCTCAAA GGAAATCCTT AGTGAAAAAT 
GGAAGAACAG AAACTCTACA TACGACGTGT AATGACGGCT CTCACCATGG CTCAAGCTGG 
ACTCCAGAGA GCCATGAGGA TCATCTCCAG GCTGAGTCCG CAGGTATCTT GTCCTGTGTG 
AAGAGAAGCC TGTGCTGGCC GCTGCACGTC AGCCAGACTG TGTGTGCTGT GCCGCGTCGT 
GTGTGTACAT GGCTGCATGG TAAAGTGTCG GCCGCTCGAG TCCACCTGAT GGACAACGCT 
GGCGATTACG CCTTCCTGTA CGAGCTGCTC AGCGTGGGTC TGCCTCTGCT GGCCATTCTC 
CATGAACAGC TGCTGCAAAT GTATGCCGAG GCTGAACCGT TCAATCTCCA GCCTCTACAG 
CACTGGCTGG AGGAGCACAT GGGCCACTGG GTCTTAGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333695.1
CDS	274..3435
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478020.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478020.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGAGGAAAG CCCAGACTCA AAAGGCCAGG 
GAGTTTCACA AAAAAAGCAT AAAGGAGAAG GCTGTGCATT TGAGCGCTCT GTTCTCTGGC 
GAGGCCTCCG CTCCTGATCA GCGCACAGTG GGCAAAGTGT CTCTGAGAGT GGGTGCTAGA 
GCCGACGCTC TGGTTACCCT GTATGAAAAT GATCATAGAC CTCAGACAAG CAGCTCTGGC 
ACTCCGCCTT CCATAAAGAG AGAGTTTCCT CAGACTCTGG GCGATGTGTG TCATGCCTGT 
AAGAAGCGTG TGTTTGTGGT GGAGAGACTC AGCGCAGAGG GATTTCACTT TCACCGGGAA 
TGTTTCCGCT GTGAGACCTG CAGAGCTCCG CTACGTCAGG GAGGACACTG CTTCCATTCT 
GAGGAAGGAA AGTTGTACTG CAAACTCCAC TTTGCTCAAA GGAAATCCTT AGTGAAAAAT 
GGAAGAACAG AAACTCTACA TACGACGTGT AATGACGGCT CTCACCATGG CTCAAGCTGG 
ACTCCAGAGA GCCATGAGGA TCATCTCCAG GCTGAGTCCG CAGGTATCTT GTCCTGTGTG 
AAGAGAAGCC TGTGCTGGCC GCTGCACGTC AGCCAGACTG TGTGTGCTGT GCCGCGTCGT 
GTGTGTACAT GGCTGCATGG TAAAGTGTCG GCCGCTCGAG TCCACCTGAT GGACAACGCT 
GGCGATTACG CCTTCCTGTA CGAGCTGCTC AGCGTGGGTC TGCCTCTGCT GGCCATTCTC 
CATGAACAGC TGCTGCAAAT GTATGCCGAG GCTGAACCGT TCAATCTCCA GCCTCTACAG 
CACTGGCTGG AGGAGCACAT GGGCCACTGG GTCTTAGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_021478017.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333692.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45812
Clone ID Related Accession (Same CDS sequence)	XM_021478017.1
Accession Version	XM_021478017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCCAT CTTCTTCTAT CCACCTCCCT 
TCTCCCAATC CATTGACCAC CACTTCATAT CCTCTAACAC AATCCACTGC TACTTCCTGT 
CCTCCACCTA TCCTTTCTTC CTGTCCATCT TTTCCAATCC AGCAGACCAG TTCTTCCACT 
GTCCACCATC ATTCTAACTC CATAATATTT CACCCCATCC CAGAATGCAC TGCTCCTATT 
CACCTTTCCT TTTCCTTCTC TTCTGCTTTC TCATTAAGCA GTAAATCAAA GTCTGAAACA 
TGCAGTGGCG TAAATATGCA AAGACAATCA GAAAGTAGCT CTCAAATGTG TGACAGCTGC 
AGTAGCATAT CATCCTTTAC TGTGAGAAAA CAAACAGATG TTCCTCAGTC AAATGAAGAC 
TTGAGTGCTA CTAAACAGCG CACAGTGGGC AAAGTGTCTC TGAGAGTGGG TGCTAGAGCC 
GACGCTCTGG TTACCCTGTA TGAAAATGAT CATAGACCTC AGACAAGCAG CTCTGGCACT 
CCGCCTTCCA TAAAGAGAGA GTTTCCTCAG ACTCTGGGCG ATGTGTGTCA TGCCTGTAAG 
AAGCGTGTGT TTGTGGTGGA GAGACTCAGC GCAGAGGGAT TTCACTTTCA CCGGGAATGT 
TTCCGCTGTG AGACCTGCAG AGCTCCGCTA CGTCAGGGAG GACACTGCTT CCATTCTGAG 
GAAGGAAAGT TGTACTGCAA ACTCCACTTT GCTCAAAGGA AATCCTTAGT GAAAAATGGA 
AGAACAGAAA CTCTACATAC GACGTGTAAT GACGGCTCTC ACCATGGCTC AAGCTGGACT 
CCAGAGAGCC ATGAGGATCA TCTCCAGGCT GAGTCCGCAG GTATCTTGTC CTGTGTGAAG 
AGAAGCCTGT GCTGGCCGCT GCACGTCAGC CAGACTGTGT GTGCTGTGCC GCGTCGTGTG 
TGTACATGGC TGCATGGTAA AGTGTCGGCC GCTCGAGTCC ACCTGATGGA CAACGCTGGC 
GATTACGCCT TCCTGTACGA GCTGCTCAGC GTGGGTCTGC CTCTGCTGGC CATTCTCCAT 
GAACAGCTGC TGCAAATGTA TGCCGAGGCT GAACCGTTCA ATCTCCAGCC TCTACAGCAC 
TGGCTGGAGG AGCACATGGG CCACTGGGTC TTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333692.1
CDS	274..4152
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQFPSIALVQHSDESSDPLFLSLHSSPLNPRRKPLHPSHTHTHAQPPHTESDTESSGSRTAVLAVTAMLQRLQRVEEEISQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQTDRSQTGLLDKQLHEEKRESAKRLLQPSSSIHLPSPNPLTTTSYPLTQSTATSCPPPILSSCPSFPIQQTSSSTVHHHSNSIIFHPIPECTAPIHLSFSFSSAFSLSSKSKSETCSGVNMQRQSESSSQMCDSCSSISSFTVRKQTDVPQSNEDLSATKQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478017.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478017.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCCAT CTTCTTCTAT CCACCTCCCT 
TCTCCCAATC CATTGACCAC CACTTCATAT CCTCTAACAC AATCCACTGC TACTTCCTGT 
CCTCCACCTA TCCTTTCTTC CTGTCCATCT TTTCCAATCC AGCAGACCAG TTCTTCCACT 
GTCCACCATC ATTCTAACTC CATAATATTT CACCCCATCC CAGAATGCAC TGCTCCTATT 
CACCTTTCCT TTTCCTTCTC TTCTGCTTTC TCATTAAGCA GTAAATCAAA GTCTGAAACA 
TGCAGTGGCG TAAATATGCA AAGACAATCA GAAAGTAGCT CTCAAATGTG TGACAGCTGC 
AGTAGCATAT CATCCTTTAC TGTGAGAAAA CAAACAGATG TTCCTCAGTC AAATGAAGAC 
TTGAGTGCTA CTAAACAGCG CACAGTGGGC AAAGTGTCTC TGAGAGTGGG TGCTAGAGCC 
GACGCTCTGG TTACCCTGTA TGAAAATGAT CATAGACCTC AGACAAGCAG CTCTGGCACT 
CCGCCTTCCA TAAAGAGAGA GTTTCCTCAG ACTCTGGGCG ATGTGTGTCA TGCCTGTAAG 
AAGCGTGTGT TTGTGGTGGA GAGACTCAGC GCAGAGGGAT TTCACTTTCA CCGGGAATGT 
TTCCGCTGTG AGACCTGCAG AGCTCCGCTA CGTCAGGGAG GACACTGCTT CCATTCTGAG 
GAAGGAAAGT TGTACTGCAA ACTCCACTTT GCTCAAAGGA AATCCTTAGT GAAAAATGGA 
AGAACAGAAA CTCTACATAC GACGTGTAAT GACGGCTCTC ACCATGGCTC AAGCTGGACT 
CCAGAGAGCC ATGAGGATCA TCTCCAGGCT GAGTCCGCAG GTATCTTGTC CTGTGTGAAG 
AGAAGCCTGT GCTGGCCGCT GCACGTCAGC CAGACTGTGT GTGCTGTGCC GCGTCGTGTG 
TGTACATGGC TGCATGGTAA AGTGTCGGCC GCTCGAGTCC ACCTGATGGA CAACGCTGGC 
GATTACGCCT TCCTGTACGA GCTGCTCAGC GTGGGTCTGC CTCTGCTGGC CATTCTCCAT 
GAACAGCTGC TGCAAATGTA TGCCGAGGCT GAACCGTTCA ATCTCCAGCC TCTACAGCAC 
TGGCTGGAGG AGCACATGGG CCACTGGGTC TTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_021478018.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333693.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X2, mRNA.

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CloneID	ODa45813
Clone ID Related Accession (Same CDS sequence)	XM_021478018.1
Accession Version	XM_021478018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCCAT CTTCTTCTAT CCACCTCCCT 
TCTCCCAATC CATTGACCAC CACTTCATAT CCTCTAACAC AATCCACTGC TACTTCCTGT 
CCTCCACCTA TCCTTTCTTC CTGTCCATCT TTTCCAATCC AGCAGCGCAC AGTGGGCAAA 
GTGTCTCTGA GAGTGGGTGC TAGAGCCGAC GCTCTGGTTA CCCTGTATGA AAATGATCAT 
AGACCTCAGA CAAGCAGCTC TGGCACTCCG CCTTCCATAA AGAGAGAGTT TCCTCAGACT 
CTGGGCGATG TGTGTCATGC CTGTAAGAAG CGTGTGTTTG TGGTGGAGAG ACTCAGCGCA 
GAGGGATTTC ACTTTCACCG GGAATGTTTC CGCTGTGAGA CCTGCAGAGC TCCGCTACGT 
CAGGGAGGAC ACTGCTTCCA TTCTGAGGAA GGAAAGTTGT ACTGCAAACT CCACTTTGCT 
CAAAGGAAAT CCTTAGTGAA AAATGGAAGA ACAGAAACTC TACATACGAC GTGTAATGAC 
GGCTCTCACC ATGGCTCAAG CTGGACTCCA GAGAGCCATG AGGATCATCT CCAGGCTGAG 
TCCGCAGGTA TCTTGTCCTG TGTGAAGAGA AGCCTGTGCT GGCCGCTGCA CGTCAGCCAG 
ACTGTGTGTG CTGTGCCGCG TCGTGTGTGT ACATGGCTGC ATGGTAAAGT GTCGGCCGCT 
CGAGTCCACC TGATGGACAA CGCTGGCGAT TACGCCTTCC TGTACGAGCT GCTCAGCGTG 
GGTCTGCCTC TGCTGGCCAT TCTCCATGAA CAGCTGCTGC AAATGTATGC CGAGGCTGAA 
CCGTTCAATC TCCAGCCTCT ACAGCACTGG CTGGAGGAGC ACATGGGCCA CTGGGTCTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333693.1
CDS	274..3879
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQFPSIALVQHSDESSDPLFLSLHSSPLNPRRKPLHPSHTHTHAQPPHTESDTESSGSRTAVLAVTAMLQRLQRVEEEISQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQTDRSQTGLLDKQLHEEKRESAKRLLQPSSSIHLPSPNPLTTTSYPLTQSTATSCPPPILSSCPSFPIQQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478018.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478018.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCCAT CTTCTTCTAT CCACCTCCCT 
TCTCCCAATC CATTGACCAC CACTTCATAT CCTCTAACAC AATCCACTGC TACTTCCTGT 
CCTCCACCTA TCCTTTCTTC CTGTCCATCT TTTCCAATCC AGCAGCGCAC AGTGGGCAAA 
GTGTCTCTGA GAGTGGGTGC TAGAGCCGAC GCTCTGGTTA CCCTGTATGA AAATGATCAT 
AGACCTCAGA CAAGCAGCTC TGGCACTCCG CCTTCCATAA AGAGAGAGTT TCCTCAGACT 
CTGGGCGATG TGTGTCATGC CTGTAAGAAG CGTGTGTTTG TGGTGGAGAG ACTCAGCGCA 
GAGGGATTTC ACTTTCACCG GGAATGTTTC CGCTGTGAGA CCTGCAGAGC TCCGCTACGT 
CAGGGAGGAC ACTGCTTCCA TTCTGAGGAA GGAAAGTTGT ACTGCAAACT CCACTTTGCT 
CAAAGGAAAT CCTTAGTGAA AAATGGAAGA ACAGAAACTC TACATACGAC GTGTAATGAC 
GGCTCTCACC ATGGCTCAAG CTGGACTCCA GAGAGCCATG AGGATCATCT CCAGGCTGAG 
TCCGCAGGTA TCTTGTCCTG TGTGAAGAGA AGCCTGTGCT GGCCGCTGCA CGTCAGCCAG 
ACTGTGTGTG CTGTGCCGCG TCGTGTGTGT ACATGGCTGC ATGGTAAAGT GTCGGCCGCT 
CGAGTCCACC TGATGGACAA CGCTGGCGAT TACGCCTTCC TGTACGAGCT GCTCAGCGTG 
GGTCTGCCTC TGCTGGCCAT TCTCCATGAA CAGCTGCTGC AAATGTATGC CGAGGCTGAA 
CCGTTCAATC TCCAGCCTCT ACAGCACTGG CTGGAGGAGC ACATGGGCCA CTGGGTCTTA 
GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_009303577.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301852.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X3, mRNA.

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CloneID	ODa45814
Clone ID Related Accession (Same CDS sequence)	XM_009303577.3 , XM_009303577.2
Accession Version	XM_009303577.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303577.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCGCA CAGTGGGCAA AGTGTCTCTG 
AGAGTGGGTG CTAGAGCCGA CGCTCTGGTT ACCCTGTATG AAAATGATCA TAGACCTCAG 
ACAAGCAGCT CTGGCACTCC GCCTTCCATA AAGAGAGAGT TTCCTCAGAC TCTGGGCGAT 
GTGTGTCATG CCTGTAAGAA GCGTGTGTTT GTGGTGGAGA GACTCAGCGC AGAGGGATTT 
CACTTTCACC GGGAATGTTT CCGCTGTGAG ACCTGCAGAG CTCCGCTACG TCAGGGAGGA 
CACTGCTTCC ATTCTGAGGA AGGAAAGTTG TACTGCAAAC TCCACTTTGC TCAAAGGAAA 
TCCTTAGTGA AAAATGGAAG AACAGAAACT CTACATACGA CGTGTAATGA CGGCTCTCAC 
CATGGCTCAA GCTGGACTCC AGAGAGCCAT GAGGATCATC TCCAGGCTGA GTCCGCAGGT 
ATCTTGTCCT GTGTGAAGAG AAGCCTGTGC TGGCCGCTGC ACGTCAGCCA GACTGTGTGT 
GCTGTGCCGC GTCGTGTGTG TACATGGCTG CATGGTAAAG TGTCGGCCGC TCGAGTCCAC 
CTGATGGACA ACGCTGGCGA TTACGCCTTC CTGTACGAGC TGCTCAGCGT GGGTCTGCCT 
CTGCTGGCCA TTCTCCATGA ACAGCTGCTG CAAATGTATG CCGAGGCTGA ACCGTTCAAT 
CTCCAGCCTC TACAGCACTG GCTGGAGGAG CACATGGGCC ACTGGGTCTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301852.1
CDS	274..3750
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQFPSIALVQHSDESSDPLFLSLHSSPLNPRRKPLHPSHTHTHAQPPHTESDTESSGSRTAVLAVTAMLQRLQRVEEEISQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQTDRSQTGLLDKQLHEEKRESAKRLLQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_009303577.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303577.3

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCGCA CAGTGGGCAA AGTGTCTCTG 
AGAGTGGGTG CTAGAGCCGA CGCTCTGGTT ACCCTGTATG AAAATGATCA TAGACCTCAG 
ACAAGCAGCT CTGGCACTCC GCCTTCCATA AAGAGAGAGT TTCCTCAGAC TCTGGGCGAT 
GTGTGTCATG CCTGTAAGAA GCGTGTGTTT GTGGTGGAGA GACTCAGCGC AGAGGGATTT 
CACTTTCACC GGGAATGTTT CCGCTGTGAG ACCTGCAGAG CTCCGCTACG TCAGGGAGGA 
CACTGCTTCC ATTCTGAGGA AGGAAAGTTG TACTGCAAAC TCCACTTTGC TCAAAGGAAA 
TCCTTAGTGA AAAATGGAAG AACAGAAACT CTACATACGA CGTGTAATGA CGGCTCTCAC 
CATGGCTCAA GCTGGACTCC AGAGAGCCAT GAGGATCATC TCCAGGCTGA GTCCGCAGGT 
ATCTTGTCCT GTGTGAAGAG AAGCCTGTGC TGGCCGCTGC ACGTCAGCCA GACTGTGTGT 
GCTGTGCCGC GTCGTGTGTG TACATGGCTG CATGGTAAAG TGTCGGCCGC TCGAGTCCAC 
CTGATGGACA ACGCTGGCGA TTACGCCTTC CTGTACGAGC TGCTCAGCGT GGGTCTGCCT 
CTGCTGGCCA TTCTCCATGA ACAGCTGCTG CAAATGTATG CCGAGGCTGA ACCGTTCAAT 
CTCCAGCCTC TACAGCACTG GCTGGAGGAG CACATGGGCC ACTGGGTCTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_009303577.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301852.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), mRNA.

Price & Availability↑

CloneID	ODa45814
Clone ID Related Accession (Same CDS sequence)	XM_009303577.3 , XM_009303577.2
Accession Version	XM_009303577.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase MICAL2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303577.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCGCA CAGTGGGCAA AGTGTCTCTG 
AGAGTGGGTG CTAGAGCCGA CGCTCTGGTT ACCCTGTATG AAAATGATCA TAGACCTCAG 
ACAAGCAGCT CTGGCACTCC GCCTTCCATA AAGAGAGAGT TTCCTCAGAC TCTGGGCGAT 
GTGTGTCATG CCTGTAAGAA GCGTGTGTTT GTGGTGGAGA GACTCAGCGC AGAGGGATTT 
CACTTTCACC GGGAATGTTT CCGCTGTGAG ACCTGCAGAG CTCCGCTACG TCAGGGAGGA 
CACTGCTTCC ATTCTGAGGA AGGAAAGTTG TACTGCAAAC TCCACTTTGC TCAAAGGAAA 
TCCTTAGTGA AAAATGGAAG AACAGAAACT CTACATACGA CGTGTAATGA CGGCTCTCAC 
CATGGCTCAA GCTGGACTCC AGAGAGCCAT GAGGATCATC TCCAGGCTGA GTCCGCAGGT 
ATCTTGTCCT GTGTGAAGAG AAGCCTGTGC TGGCCGCTGC ACGTCAGCCA GACTGTGTGT 
GCTGTGCCGC GTCGTGTGTG TACATGGCTG CATGGTAAAG TGTCGGCCGC TCGAGTCCAC 
CTGATGGACA ACGCTGGCGA TTACGCCTTC CTGTACGAGC TGCTCAGCGT GGGTCTGCCT 
CTGCTGGCCA TTCTCCATGA ACAGCTGCTG CAAATGTATG CCGAGGCTGA ACCGTTCAAT 
CTCCAGCCTC TACAGCACTG GCTGGAGGAG CACATGGGCC ACTGGGTCTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301852.1
CDS	259..3735
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQFPSIALVQHSDESSDPLFLSLHSSPLNPRRKPLHPSHTHTHAQPPHTESDTESSGSRTAVLAVTAMLQRLQRVEEEISQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQTDRSQTGLLDKQLHEEKRESAKRLLQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_009303577.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303577.2

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGAC GGATAGATCT CAGACTGGGC TGTTAGACAA GCAGCTTCAT 
GAAGAGAAGA GAGAGTCAGC AAAAAGATTA CTGCAGCGCA CAGTGGGCAA AGTGTCTCTG 
AGAGTGGGTG CTAGAGCCGA CGCTCTGGTT ACCCTGTATG AAAATGATCA TAGACCTCAG 
ACAAGCAGCT CTGGCACTCC GCCTTCCATA AAGAGAGAGT TTCCTCAGAC TCTGGGCGAT 
GTGTGTCATG CCTGTAAGAA GCGTGTGTTT GTGGTGGAGA GACTCAGCGC AGAGGGATTT 
CACTTTCACC GGGAATGTTT CCGCTGTGAG ACCTGCAGAG CTCCGCTACG TCAGGGAGGA 
CACTGCTTCC ATTCTGAGGA AGGAAAGTTG TACTGCAAAC TCCACTTTGC TCAAAGGAAA 
TCCTTAGTGA AAAATGGAAG AACAGAAACT CTACATACGA CGTGTAATGA CGGCTCTCAC 
CATGGCTCAA GCTGGACTCC AGAGAGCCAT GAGGATCATC TCCAGGCTGA GTCCGCAGGT 
ATCTTGTCCT GTGTGAAGAG AAGCCTGTGC TGGCCGCTGC ACGTCAGCCA GACTGTGTGT 
GCTGTGCCGC GTCGTGTGTG TACATGGCTG CATGGTAAAG TGTCGGCCGC TCGAGTCCAC 
CTGATGGACA ACGCTGGCGA TTACGCCTTC CTGTACGAGC TGCTCAGCGT GGGTCTGCCT 
CTGCTGGCCA TTCTCCATGA ACAGCTGCTG CAAATGTATG CCGAGGCTGA ACCGTTCAAT 
CTCCAGCCTC TACAGCACTG GCTGGAGGAG CACATGGGCC ACTGGGTCTT AGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical2a ( XM_021478019.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333694.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X4, mRNA.

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CloneID	ODa45815
Clone ID Related Accession (Same CDS sequence)	XM_021478019.1
Accession Version	XM_021478019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	F-actin-methionine sulfoxide oxidase MICAL2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGCG CACAGTGGGC AAAGTGTCTC TGAGAGTGGG TGCTAGAGCC 
GACGCTCTGG TTACCCTGTA TGAAAATGAT CATAGACCTC AGACAAGCAG CTCTGGCACT 
CCGCCTTCCA TAAAGAGAGA GTTTCCTCAG ACTCTGGGCG ATGTGTGTCA TGCCTGTAAG 
AAGCGTGTGT TTGTGGTGGA GAGACTCAGC GCAGAGGGAT TTCACTTTCA CCGGGAATGT 
TTCCGCTGTG AGACCTGCAG AGCTCCGCTA CGTCAGGGAG GACACTGCTT CCATTCTGAG 
GAAGGAAAGT TGTACTGCAA ACTCCACTTT GCTCAAAGGA AATCCTTAGT GAAAAATGGA 
AGAACAGAAA CTCTACATAC GACGTGTAAT GACGGCTCTC ACCATGGCTC AAGCTGGACT 
CCAGAGAGCC ATGAGGATCA TCTCCAGGCT GAGTCCGCAG GTATCTTGTC CTGTGTGAAG 
AGAAGCCTGT GCTGGCCGCT GCACGTCAGC CAGACTGTGT GTGCTGTGCC GCGTCGTGTG 
TGTACATGGC TGCATGGTAA AGTGTCGGCC GCTCGAGTCC ACCTGATGGA CAACGCTGGC 
GATTACGCCT TCCTGTACGA GCTGCTCAGC GTGGGTCTGC CTCTGCTGGC CATTCTCCAT 
GAACAGCTGC TGCAAATGTA TGCCGAGGCT GAACCGTTCA ATCTCCAGCC TCTACAGCAC 
TGGCTGGAGG AGCACATGGG CCACTGGGTC TTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333694.1
CDS	274..3672
Translation	MMGEAEDEKNRTGRLFENFVQATDCKSTLQAFNILCGSLELDPLEHSSFYGSLKSSLTCWKAKALWSKLDKRASHKEYKKGRACEGIKCLIIGGGPCGLRTAIELAFLGAKVVVIEKRDTFSRNNVLHLWPYTIHDLRALGAKKFYGKFCAGAIDHISIRQLQLMLLKIALILGVEFHVNVEFVKLAEPPEDQENEGPGWRADIRPADHPVADFDFDVVVGADGRRNSLEGFRRKEFRGKLAIAITANFTNRNTTAEAKVEEISGVAFIFNQKFFQDLRQETGIDLENIVYYKDNTHYFVMTAKKQSLLDKGVIIHDYIDTEALLNSENVNQEALLVYAREAADYATHYQLPTLDYAMNHCAQPDVAMFDFTSMYASENAALVRERFGHSVLVALVGDSLLEPFWPMGTGCARGFLAAFDTAWMVKSWAQGRNPLEVLAERESLYRLLPQTTPENIAKNFDLYTIDPGTRYPNLNSNCVRPHQVRNNYICGEMKLCSLERASTIRRPVNLARRESEIRPGRLLTWCQKQTEGYRDVNVTDLTSCWSNGLALCALIHRFRPQLIDYDSLNEQDCARNLQLAFDVAEKEFGIKPFITGKELASGQEPDKSSMSIYLSKFYELFRGTPLPPSDSKRDDDANNEKCPSGSPKSVYKSLSLQPRKRIPKNDKKVEDTDAISKRRRKLNYLEETTKSYNQSSSVSSEGRELKENKVKFMATQLLAKFEDSTASCSLRRQSDCNGERPPRPPSLDMTDSSFFNIIKKKVPPPPPPKKQFPSIALVQHSDESSDPLFLSLHSSPLNPRRKPLHPSHTHTHAQPPHTESDTESSGSRTAVLAVTAMLQRLQRVEEEISQRKAQTQKAREFHKKSIKEKAVHLSALFSGEASAPDQRTVGKVSLRVGARADALVTLYENDHRPQTSSSGTPPSIKREFPQTLGDVCHACKKRVFVVERLSAEGFHFHRECFRCETCRAPLRQGGHCFHSEEGKLYCKLHFAQRKSLVKNGRTETLHTTCNDGSHHGSSWTPESHEDHLQAESAGILSCVKRSLCWPLHVSQTVCAVPRRVCTWLHGKVSAARVHLMDNAGDYAFLYELLSVGLPLLAILHEQLLQMYAEAEPFNLQPLQHWLEEHMGHWVLA

Target ORF information:

RefSeq Version	XM_021478019.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 2a (mical2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478019.1

ORF Insert Sequence:

ATGATGGGTG AAGCTGAAGA TGAGAAGAAC CGGACTGGAC GGCTGTTTGA GAACTTTGTC 
CAGGCCACGG ACTGTAAAAG CACTCTGCAG GCCTTCAATA TTTTGTGCGG TTCCCTGGAG 
CTGGACCCTC TGGAGCACAG CAGCTTCTAC GGCAGCCTGA AGAGCTCGCT GACCTGCTGG 
AAAGCCAAAG CTCTGTGGAG TAAACTGGAC AAGAGAGCCA GCCATAAAGA GTACAAGAAG 
GGCAGAGCAT GTGAGGGCAT TAAGTGCTTG ATAATTGGAG GAGGCCCCTG CGGCTTGCGG 
ACGGCCATCG AGTTAGCTTT CCTCGGAGCC AAAGTGGTGG TGATAGAGAA GAGAGACACG 
TTCTCCAGAA ACAACGTCCT GCATCTCTGG CCCTACACCA TTCATGACCT CAGGGCTTTG 
GGAGCCAAGA AGTTCTATGG AAAGTTCTGC GCTGGAGCGA TTGACCATAT CAGCATTCGG 
CAGCTCCAGC TCATGCTCCT GAAAATCGCT CTGATTCTTG GTGTGGAGTT TCATGTTAAT 
GTGGAGTTCG TCAAGCTGGC TGAGCCTCCA GAGGACCAGG AGAATGAAGG GCCGGGCTGG 
AGGGCAGACA TTCGGCCTGC CGATCACCCT GTGGCCGACT TTGACTTTGA TGTGGTGGTG 
GGAGCCGATG GAAGAAGAAA TTCTTTAGAA GGGTTTAGGA GGAAGGAGTT TCGTGGGAAA 
CTGGCTATCG CCATTACGGC AAACTTTACC AACAGAAACA CCACAGCTGA GGCAAAGGTG 
GAGGAGATAA GCGGAGTCGC GTTCATCTTT AACCAAAAAT TCTTTCAGGA TCTTAGACAG 
GAAACCGGGA TTGATCTAGA GAACATTGTT TACTACAAAG ACAACACACA TTACTTTGTC 
ATGACTGCTA AGAAGCAAAG TCTGCTGGAC AAGGGTGTCA TCATTCATGA TTATATCGAC 
ACTGAGGCAC TTCTGAACAG TGAGAATGTA AACCAAGAGG CTTTGCTAGT GTATGCACGT 
GAGGCAGCCG ACTACGCCAC ACACTACCAG CTGCCCACCC TAGATTACGC CATGAACCAC 
TGCGCGCAGC CGGACGTGGC CATGTTCGAC TTCACCAGCA TGTACGCATC CGAAAACGCC 
GCTCTGGTCA GGGAGAGGTT TGGTCATAGT GTGCTGGTGG CTCTAGTGGG AGACAGTCTT 
CTTGAGCCCT TCTGGCCAAT GGGGACAGGC TGTGCCCGAG GCTTCCTTGC AGCTTTTGAC 
ACGGCTTGGA TGGTGAAAAG TTGGGCGCAG GGCAGAAATC CACTGGAGGT GCTGGCAGAG 
AGGGAGAGTC TGTACCGATT GCTACCTCAG ACAACACCAG AAAACATTGC CAAAAACTTT 
GATTTGTACA CCATAGACCC TGGAACTCGA TATCCAAACC TGAATTCAAA CTGTGTGCGG 
CCACACCAGG TCCGCAACAA TTATATCTGT GGCGAGATGA AGCTGTGCTC TCTGGAACGA 
GCCTCCACCA TCCGACGTCC TGTCAACTTG GCTAGGAGAG AGTCTGAGAT CAGACCGGGT 
CGGTTGCTGA CGTGGTGTCA GAAGCAGACT GAGGGCTACA GGGATGTGAA TGTGACTGAC 
CTTACTTCCT GCTGGAGCAA TGGCCTGGCT CTCTGTGCGC TCATCCACCG CTTCAGACCG 
CAGCTCATTG ATTACGACTC TCTGAATGAG CAAGACTGTG CCAGAAACCT TCAGCTGGCA 
TTTGATGTGG CCGAGAAGGA GTTTGGCATT AAGCCGTTCA TCACAGGGAA AGAGCTGGCC 
TCGGGTCAGG AGCCGGACAA ATCCAGCATG AGCATCTACC TCTCCAAATT CTACGAGCTC 
TTCCGTGGAA CACCTCTACC TCCTTCAGAT TCAAAGCGAG ATGACGATGC AAATAATGAA 
AAATGTCCAT CAGGCTCTCC CAAATCTGTT TACAAATCAC TGAGTTTACA GCCCCGTAAA 
CGCATACCAA AGAATGATAA AAAGGTTGAA GACACTGATG CCATATCTAA GAGAAGGCGG 
AAATTAAATT ACTTGGAAGA GACAACCAAA TCCTACAATC AGAGCTCTTC TGTATCCAGC 
GAGGGGAGGG AACTCAAGGA AAACAAGGTC AAGTTCATGG CCACTCAACT TCTAGCCAAA 
TTCGAAGACA GTACAGCAAG CTGCTCTCTT CGCAGACAGT CTGACTGTAA TGGTGAGAGG 
CCTCCTAGAC CTCCATCGCT TGATATGACT GATAGTTCCT TCTTTAATAT TATCAAGAAG 
AAGGTTCCTC CGCCGCCACC TCCTAAAAAA CAGTTTCCAT CCATTGCCCT TGTGCAACAC 
AGCGATGAGT CTTCAGATCC TCTGTTCCTC TCGCTTCATT CCAGTCCTCT GAATCCACGG 
AGGAAACCAC TTCACCCGTC ACACACACAC ACACATGCTC AACCACCTCA CACAGAGTCA 
GACACAGAGA GCTCAGGAAG CCGCACAGCA GTGCTGGCTG TGACCGCGAT GCTCCAGCGC 
CTCCAGAGGG TGGAAGAAGA GATCAGCCAG AGGAAAGCCC AGACTCAAAA GGCCAGGGAG 
TTTCACAAAA AAAGCATAAA GGAGAAGGCT GTGCATTTGA GCGCTCTGTT CTCTGGCGAG 
GCCTCCGCTC CTGATCAGCG CACAGTGGGC AAAGTGTCTC TGAGAGTGGG TGCTAGAGCC 
GACGCTCTGG TTACCCTGTA TGAAAATGAT CATAGACCTC AGACAAGCAG CTCTGGCACT 
CCGCCTTCCA TAAAGAGAGA GTTTCCTCAG ACTCTGGGCG ATGTGTGTCA TGCCTGTAAG 
AAGCGTGTGT TTGTGGTGGA GAGACTCAGC GCAGAGGGAT TTCACTTTCA CCGGGAATGT 
TTCCGCTGTG AGACCTGCAG AGCTCCGCTA CGTCAGGGAG GACACTGCTT CCATTCTGAG 
GAAGGAAAGT TGTACTGCAA ACTCCACTTT GCTCAAAGGA AATCCTTAGT GAAAAATGGA 
AGAACAGAAA CTCTACATAC GACGTGTAAT GACGGCTCTC ACCATGGCTC AAGCTGGACT 
CCAGAGAGCC ATGAGGATCA TCTCCAGGCT GAGTCCGCAG GTATCTTGTC CTGTGTGAAG 
AGAAGCCTGT GCTGGCCGCT GCACGTCAGC CAGACTGTGT GTGCTGTGCC GCGTCGTGTG 
TGTACATGGC TGCATGGTAA AGTGTCGGCC GCTCGAGTCC ACCTGATGGA CAACGCTGGC 
GATTACGCCT TCCTGTACGA GCTGCTCAGC GTGGGTCTGC CTCTGCTGGC CATTCTCCAT 
GAACAGCTGC TGCAAATGTA TGCCGAGGCT GAACCGTTCA ATCTCCAGCC TCTACAGCAC 
TGGCTGGAGG AGCACATGGG CCACTGGGTC TTAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mical2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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