ankle2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankle2
Entrez Gene ID	559297
Full Name	ankyrin repeat and LEM domain containing 2
Synonyms	si:dkey-215k6.5,wu:fi34a12
General protein information	Preferred Names ankyrin repeat and LEM domain containing 2 Names ankyrin repeat and LEM domain-containing protein 2 fi34a12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126395.2	NP_001119867.1	ankyrin repeat and LEM domain-containing protein 2
XM_005165080.3	XP_005165137.1	ankyrin repeat and LEM domain-containing protein 2 isoform X1
XM_017356107.1	XP_017211596.1	ankyrin repeat and LEM domain-containing protein 2 isoform X2
XM_005165080.4	XP_005165137.1	ankyrin repeat and LEM domain-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ankle2	NP_001041366.1
Homo sapiens (human)	ANKLE2	NP_055929.1
Bos taurus (cattle)	ANKLE2	XP_002694494.2
	ANKLE2	XP_001087028.1
Danio rerio (zebrafish)	ankle2	NP_001119867.1
Xenopus tropicalis (tropical clawed frog)	ankle2	XP_004910629.1
Canis lupus familiaris (dog)	ANKLE2	XP_852228.2
Pan troglodytes (chimpanzee)	ANKLE2	XP_001152324.2
Mus musculus (house mouse)	Ankle2	NP_001240743.1
Gallus gallus (chicken)	ANKLE2	XP_415084.4

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

15000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis.
Lo J, Lee S, Xu M, Liu F, Ruan H, Eun A, He Y, Ma W, Wang W, Wen Z, Peng J
Genome research13(3)455-66(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ankle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10210	NM_001126395.2 Latest version!	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10210	XM_005165080.4 Latest version!	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa59091	XM_017356107.1 Latest version!	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa10210	XM_005165080.3	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankle2 ( NM_001126395.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001119867.1 Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), mRNA.

Price & Availability↑

CloneID	ODa10210
Clone ID Related Accession (Same CDS sequence)	NM_001126395.2 , XM_005165080.3 , XM_005165080.4
Accession Version	NM_001126395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163479.1. On or before Jul 31, 2008 this sequence version replaced XM_682629.3, NM_001126395.1. ##Evidence-Data-START## Transcript exon combination :: BC163479.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119867.1
CDS	159..3044
Translation	MDAVLSRLKGLTADELREEIIKANLKCGPITATTRAIFERKLARALVESAGLTEPDASGDNSATLDRSVVPSSAASATPDAITGEDGDFGYGMGLNPPEEEALSGTVCACNVENTQTPSKTAQVSPTFYGVCPLWEDVLARNERAHVYTDKKEALQAVKMMKGARFKAFSNREDAEKFAKGICDYYPSPSKSTPCVSPVKPGLVFSKDHPSMIEADTINREKANSFKSPRTQDLTAKLRKAVEKGDELGFTELVWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGIVQILLDTLENPEFMRLMYPDDLESMLQKRIRYIVDLYLNTPDKAGFETPLHFACKFGCPEVVNILCSHPDIDKNCKNKYNQKPCEVICERMKEKGKVQEVKQKINEYLEERLYIPLLRATDNSSQAVIGAPWSPEPMESLSPWLPRSPKDPVMAVRAFAGPLSPSKADEFRRAWKTPPRERADHFHNILKSDPDRGAERVGRDLAHELGHPWAEYWDFLDSFVDLQSTEGLKMLEEYLSKKDFRERAHEEAGENETSNRFKTPSPGKLKKFCNSISVGAFLDEGDDISLEEIKNRQNAALTSISSVCSKDGLQGAVGGLEFHILPVSHSADLIEAAAEQDLLLSEKSMISSSKNGLCEQGSGDRTPNGDKVSPRICGSSNSCMLSPISNLMAEFERMSLLDEPDRTNRERRRSGGKRQTTELTSGLGRLSLSSEDDSVFEKDRTSALRAAENDGTERRNEVDVRSSASSDEYFLASERLEPINQQTIDTSGCERMICARSKSWDHGGRDLSSSGSSSSYRSLDSSQDFILRTPPRVTKGLFIEGDSPTKLDREVLSALGVSDFDPLKFPSIHKWKSTIQTYSQSEMQSWLTPAVHKSHLRALSATPGSPVSGLMSPVSRFSPARHMGSPDNCSPGRYSPAHSTYSPAHPNYSPVSYIQRIRLKHFGDTPI

Target ORF information:

RefSeq Version	NM_001126395.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126395.2

ORF Insert Sequence:

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_005165080.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165137.1 Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10210
Clone ID Related Accession (Same CDS sequence)	NM_001126395.2 , XM_005165080.3 , XM_005165080.4
Accession Version	XM_005165080.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165080.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165137.1
CDS	264..3149
Translation	MDAVLSRLKGLTADELREEIIKANLKCGPITATTRAIFERKLARALVESAGLTEPDASGDNSATLDRSVVPSSAASATPDAITGEDGDFGYGMGLNPPEEEALSGTVCACNVENTQTPSKTAQVSPTFYGVCPLWEDVLARNERAHVYTDKKEALQAVKMMKGARFKAFSNREDAEKFAKGICDYYPSPSKSTPCVSPVKPGLVFSKDHPSMIEADTINREKANSFKSPRTQDLTAKLRKAVEKGDELGFTELVWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGIVQILLDTLENPEFMRLMYPDDLESMLQKRIRYIVDLYLNTPDKAGFETPLHFACKFGCPEVVNILCSHPDIDKNCKNKYNQKPCEVICERMKEKGKVQEVKQKINEYLEERLYIPLLRATDNSSQAVIGAPWSPEPMESLSPWLPRSPKDPVMAVRAFAGPLSPSKADEFRRAWKTPPRERADHFHNILKSDPDRGAERVGRDLAHELGHPWAEYWDFLDSFVDLQSTEGLKMLEEYLSKKDFRERAHEEAGENETSNRFKTPSPGKLKKFCNSISVGAFLDEGDDISLEEIKNRQNAALTSISSVCSKDGLQGAVGGLEFHILPVSHSADLIEAAAEQDLLLSEKSMISSSKNGLCEQGSGDRTPNGDKVSPRICGSSNSCMLSPISNLMAEFERMSLLDEPDRTNRERRRSGGKRQTTELTSGLGRLSLSSEDDSVFEKDRTSALRAAENDGTERRNEVDVRSSASSDEYFLASERLEPINQQTIDTSGCERMICARSKSWDHGGRDLSSSGSSSSYRSLDSSQDFILRTPPRVTKGLFIEGDSPTKLDREVLSALGVSDFDPLKFPSIHKWKSTIQTYSQSEMQSWLTPAVHKSHLRALSATPGSPVSGLMSPVSRFSPARHMGSPDNCSPGRYSPAHSTYSPAHPNYSPVSYIQRIRLKHFGDTPI

Target ORF information:

RefSeq Version	XM_005165080.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165080.3

ORF Insert Sequence:

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_005165080.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165137.1 Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10210
Clone ID Related Accession (Same CDS sequence)	NM_001126395.2 , XM_005165080.3 , XM_005165080.4
Accession Version	XM_005165080.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165080.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165137.1
CDS	287..3172
Translation	MDAVLSRLKGLTADELREEIIKANLKCGPITATTRAIFERKLARALVESAGLTEPDASGDNSATLDRSVVPSSAASATPDAITGEDGDFGYGMGLNPPEEEALSGTVCACNVENTQTPSKTAQVSPTFYGVCPLWEDVLARNERAHVYTDKKEALQAVKMMKGARFKAFSNREDAEKFAKGICDYYPSPSKSTPCVSPVKPGLVFSKDHPSMIEADTINREKANSFKSPRTQDLTAKLRKAVEKGDELGFTELVWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGIVQILLDTLENPEFMRLMYPDDLESMLQKRIRYIVDLYLNTPDKAGFETPLHFACKFGCPEVVNILCSHPDIDKNCKNKYNQKPCEVICERMKEKGKVQEVKQKINEYLEERLYIPLLRATDNSSQAVIGAPWSPEPMESLSPWLPRSPKDPVMAVRAFAGPLSPSKADEFRRAWKTPPRERADHFHNILKSDPDRGAERVGRDLAHELGHPWAEYWDFLDSFVDLQSTEGLKMLEEYLSKKDFRERAHEEAGENETSNRFKTPSPGKLKKFCNSISVGAFLDEGDDISLEEIKNRQNAALTSISSVCSKDGLQGAVGGLEFHILPVSHSADLIEAAAEQDLLLSEKSMISSSKNGLCEQGSGDRTPNGDKVSPRICGSSNSCMLSPISNLMAEFERMSLLDEPDRTNRERRRSGGKRQTTELTSGLGRLSLSSEDDSVFEKDRTSALRAAENDGTERRNEVDVRSSASSDEYFLASERLEPINQQTIDTSGCERMICARSKSWDHGGRDLSSSGSSSSYRSLDSSQDFILRTPPRVTKGLFIEGDSPTKLDREVLSALGVSDFDPLKFPSIHKWKSTIQTYSQSEMQSWLTPAVHKSHLRALSATPGSPVSGLMSPVSRFSPARHMGSPDNCSPGRYSPAHSTYSPAHPNYSPVSYIQRIRLKHFGDTPI

Target ORF information:

RefSeq Version	XM_005165080.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165080.4

ORF Insert Sequence:

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGG CAAACTGAAG 
AAGTTCTGTA ATTCCATCTC CGTGGGGGCT TTTCTAGATG AGGGTGATGA CATCAGCCTG 
GAGGAGATCA AGAACCGGCA GAACGCTGCT CTCACCAGTA TTTCTTCTGT GTGCTCCAAA 
GACGGCCTGC AGGGGGCAGT AGGAGGACTG GAGTTTCACA TCCTCCCAGT GTCCCACAGC 
GCTGACCTCA TAGAAGCGGC GGCAGAGCAG GACCTGCTGC TCTCAGAGAA ATCCATGATC 
TCCAGCAGTA AAAATGGCCT TTGCGAACAG GGCTCCGGTG ATAGGACTCC AAACGGGGAC 
AAAGTATCGC CTCGTATCTG CGGTTCCTCC AATTCCTGCA TGCTGTCACC AATCTCAAAC 
TTAATGGCAG AGTTTGAGAG GATGTCTTTG CTGGATGAGC CTGATAGGAC AAACAGAGAG 
AGGAGGAGGA GCGGAGGGAA ACGTCAGACC ACAGAGCTCA CCTCTGGACT GGGACGCCTT 
TCGCTGTCTT CTGAAGACGA CTCTGTGTTT GAGAAAGACA GGACGTCTGC TTTGCGGGCA 
GCAGAGAATG ATGGAACAGA GAGGAGAAAT GAGGTGGACG TGAGGTCCAG TGCGAGTTCA 
GATGAGTACT TTCTGGCCAG TGAGCGGCTG GAACCTATAA ACCAACAGAC TATAGATACT 
TCAGGGTGCG AGCGCATGAT CTGCGCCAGG TCAAAGTCCT GGGATCATGG GGGAAGAGAC 
CTCAGCTCCT CGGGGTCTTC CAGCTCCTAC AGATCACTCG ACAGCTCTCA GGACTTCATA 
CTGCGGACTC CTCCAAGAGT GACCAAAGGC CTCTTCATTG AAGGGGATTC ACCCACCAAG 
TTGGACAGAG AGGTTTTGTC AGCTTTAGGA GTCTCGGACT TCGACCCTCT AAAGTTTCCC 
AGCATTCACA AATGGAAGAG CACAATTCAG ACATACTCAC AGTCAGAAAT GCAAAGCTGG 
CTGACTCCTG CAGTGCACAA AAGCCATCTG AGGGCTCTCT CGGCCACTCC TGGCTCTCCA 
GTGAGCGGTT TGATGTCCCC CGTGAGCCGC TTCAGCCCAG CTCGACACAT GGGCTCGCCA 
GACAACTGCA GTCCCGGACG CTACAGTCCA GCACACTCCA CCTACAGCCC CGCTCACCCC 
AACTACAGCC CTGTCAGCTA CATTCAACGC ATCCGCCTCA AACATTTCGG AGATACTCCT 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_017356107.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211596.1 Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59091
Clone ID Related Accession (Same CDS sequence)	XM_017356107.1
Accession Version	XM_017356107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGA TGAGGGTGAT 
GACATCAGCC TGGAGGAGAT CAAGAACCGG CAGAACGCTG CTCTCACCAG TATTTCTTCT 
GTGTGCTCCA AAGACGGCCT GCAGGGGGCA GTAGGAGGAC TGGAGTTTCA CATCCTCCCA 
GTGTCCCACA GCGCTGACCT CATAGAAGCG GCGGCAGAGC AGGACCTGCT GCTCTCAGAG 
AAATCCATGA TCTCCAGCAG TAAAAATGGC CTTTGCGAAC AGGGCTCCGG TGATAGGACT 
CCAAACGGGG ACAAAGTATC GCCTCGTATC TGCGGTTCCT CCAATTCCTG CATGCTGTCA 
CCAATCTCAA ACTTAATGGC AGAGTTTGAG AGGATGTCTT TGCTGGATGA GCCTGATAGG 
ACAAACAGAG AGAGGAGGAG GAGCGGAGGG AAACGTCAGA CCACAGAGCT CACCTCTGGA 
CTGGGACGCC TTTCGCTGTC TTCTGAAGAC GACTCTGTGT TTGAGAAAGA CAGGACGTCT 
GCTTTGCGGG CAGCAGAGAA TGATGGAACA GAGAGGAGAA ATGAGGTGGA CGTGAGGTCC 
AGTGCGAGTT CAGATGAGTA CTTTCTGGCC AGTGAGCGGC TGGAACCTAT AAACCAACAG 
ACTATAGATA CTTCAGGGTG CGAGCGCATG ATCTGCGCCA GGTCAAAGTC CTGGGATCAT 
GGGGGAAGAG ACCTCAGCTC CTCGGGGTCT TCCAGCTCCT ACAGATCACT CGACAGCTCT 
CAGGACTTCA TACTGCGGAC TCCTCCAAGA GTGACCAAAG GCCTCTTCAT TGAAGGGGAT 
TCACCCACCA AGTTGGACAG AGAGGTTTTG TCAGCTTTAG GAGTCTCGGA CTTCGACCCT 
CTAAAGTTTC CCAGCATTCA CAAATGGAAG AGCACAATTC AGACATACTC ACAGTCAGAA 
ATGCAAAGCT GGCTGACTCC TGCAGTGCAC AAAAGCCATC TGAGGGCTCT CTCGGCCACT 
CCTGGCTCTC CAGTGAGCGG TTTGATGTCC CCCGTGAGCC GCTTCAGCCC AGCTCGACAC 
ATGGGCTCGC CAGACAACTG CAGTCCCGGA CGCTACAGTC CAGCACACTC CACCTACAGC 
CCCGCTCACC CCAACTACAG CCCTGTCAGC TACATTCAAC GCATCCGCCT CAAACATTTC 
GGAGATACTC CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211596.1
CDS	264..3101
Translation	MDAVLSRLKGLTADELREEIIKANLKCGPITATTRAIFERKLARALVESAGLTEPDASGDNSATLDRSVVPSSAASATPDAITGEDGDFGYGMGLNPPEEEALSGTVCACNVENTQTPSKTAQVSPTFYGVCPLWEDVLARNERAHVYTDKKEALQAVKMMKGARFKAFSNREDAEKFAKGICDYYPSPSKSTPCVSPVKPGLVFSKDHPSMIEADTINREKANSFKSPRTQDLTAKLRKAVEKGDELGFTELVWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPGIVQILLDTLENPEFMRLMYPDDLESMLQKRIRYIVDLYLNTPDKAGFETPLHFACKFGCPEVVNILCSHPDIDKNCKNKYNQKPCEVICERMKEKGKVQEVKQKINEYLEERLYIPLLRATDNSSQAVIGAPWSPEPMESLSPWLPRSPKDPVMAVRAFAGPLSPSKADEFRRAWKTPPRERADHFHNILKSDPDRGAERVGRDLAHELGHPWAEYWDFLDSFVDLQSTEGLKMLEEYLSKKDFRERAHEEAGENETSNRFKTPSPDEGDDISLEEIKNRQNAALTSISSVCSKDGLQGAVGGLEFHILPVSHSADLIEAAAEQDLLLSEKSMISSSKNGLCEQGSGDRTPNGDKVSPRICGSSNSCMLSPISNLMAEFERMSLLDEPDRTNRERRRSGGKRQTTELTSGLGRLSLSSEDDSVFEKDRTSALRAAENDGTERRNEVDVRSSASSDEYFLASERLEPINQQTIDTSGCERMICARSKSWDHGGRDLSSSGSSSSYRSLDSSQDFILRTPPRVTKGLFIEGDSPTKLDREVLSALGVSDFDPLKFPSIHKWKSTIQTYSQSEMQSWLTPAVHKSHLRALSATPGSPVSGLMSPVSRFSPARHMGSPDNCSPGRYSPAHSTYSPAHPNYSPVSYIQRIRLKHFGDTPI

Target ORF information:

RefSeq Version	XM_017356107.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356107.1

ORF Insert Sequence:

ATGGACGCTG TCCTGAGCAG GTTGAAAGGC CTCACTGCTG ATGAACTGAG GGAGGAGATC 
ATTAAAGCCA ACTTGAAATG TGGCCCCATC ACTGCCACGA CTAGAGCCAT ATTTGAGCGC 
AAGCTTGCCA GGGCTCTAGT GGAGAGTGCT GGACTTACTG AGCCTGATGC CAGTGGTGAT 
AACAGTGCCA CGTTGGACAG GAGTGTGGTG CCATCCAGTG CCGCTTCAGC AACCCCAGAT 
GCAATCACAG GCGAAGATGG GGATTTCGGG TACGGCATGG GCCTTAATCC ACCAGAGGAA 
GAGGCTCTGA GTGGAACAGT GTGCGCCTGT AATGTGGAGA ACACGCAGAC TCCCTCTAAA 
ACTGCACAGG TGTCACCCAC CTTCTACGGA GTGTGTCCAT TGTGGGAGGA TGTGCTGGCT 
AGGAATGAGA GAGCCCATGT GTACACGGAT AAGAAAGAGG CTTTGCAGGC TGTTAAAATG 
ATGAAGGGGG CTCGGTTTAA AGCTTTCTCC AATCGTGAGG ATGCTGAGAA GTTTGCCAAA 
GGCATTTGTG ACTACTACCC TTCGCCTAGT AAAAGCACTC CCTGTGTTTC TCCTGTCAAA 
CCCGGCCTGG TGTTTAGCAA AGACCACCCT TCTATGATAG AGGCAGACAC AATCAACAGG 
GAGAAAGCAA ACAGTTTTAA GAGCCCTCGG ACACAGGATT TGACAGCTAA GCTGAGGAAG 
GCTGTGGAAA AGGGAGACGA GTTGGGATTT ACTGAGCTGG TGTGGAGCAA CCCTCGCTAC 
CTCATCGGGT CAGGAGACAA CCCTACTGTA GTCCAGGAGG GCTGCAGGTA TAACGTAATG 
CATGTGGCTG CTAAAGAGAA CCAGCCAGGG ATTGTTCAGA TCCTGTTGGA CACTCTTGAG 
AACCCAGAAT TCATGCGGCT TATGTACCCA GATGACCTGG AGAGCATGCT GCAGAAACGC 
ATCCGCTATA TCGTAGACCT GTACCTTAAT ACACCAGACA AAGCTGGGTT TGAGACACCT 
CTTCACTTCG CATGCAAGTT TGGCTGCCCA GAGGTTGTAA ACATACTCTG TTCTCATCCA 
GACATTGACA AGAACTGCAA GAACAAGTAT AACCAGAAAC CCTGTGAGGT TATTTGTGAA 
AGAATGAAAG AAAAGGGTAA AGTTCAAGAA GTCAAACAGA AAATAAATGA ATATTTAGAA 
GAGCGATTGT ACATCCCTTT GCTGCGAGCC ACTGATAACT CCTCCCAGGC TGTGATTGGT 
GCTCCTTGGT CTCCTGAGCC AATGGAATCC CTTTCGCCAT GGTTACCCAG AAGTCCAAAG 
GATCCAGTTA TGGCAGTCAG GGCCTTTGCT GGACCTTTAA GTCCATCCAA AGCGGATGAG 
TTCAGACGAG CGTGGAAAAC TCCTCCCAGA GAGCGGGCAG ACCATTTTCA CAACATCCTG 
AAGTCTGACC CGGACCGTGG AGCAGAAAGA GTTGGCAGAG ATCTGGCCCA TGAACTCGGT 
CACCCCTGGG CAGAGTACTG GGATTTCCTG GATAGTTTTG TGGATCTGCA GTCAACTGAA 
GGCCTGAAAA TGCTTGAAGA ATACCTCAGT AAAAAGGACT TCAGGGAGCG AGCTCATGAA 
GAGGCTGGAG AGAATGAAAC CAGCAACAGG TTTAAAACAC CCTCTCCAGA TGAGGGTGAT 
GACATCAGCC TGGAGGAGAT CAAGAACCGG CAGAACGCTG CTCTCACCAG TATTTCTTCT 
GTGTGCTCCA AAGACGGCCT GCAGGGGGCA GTAGGAGGAC TGGAGTTTCA CATCCTCCCA 
GTGTCCCACA GCGCTGACCT CATAGAAGCG GCGGCAGAGC AGGACCTGCT GCTCTCAGAG 
AAATCCATGA TCTCCAGCAG TAAAAATGGC CTTTGCGAAC AGGGCTCCGG TGATAGGACT 
CCAAACGGGG ACAAAGTATC GCCTCGTATC TGCGGTTCCT CCAATTCCTG CATGCTGTCA 
CCAATCTCAA ACTTAATGGC AGAGTTTGAG AGGATGTCTT TGCTGGATGA GCCTGATAGG 
ACAAACAGAG AGAGGAGGAG GAGCGGAGGG AAACGTCAGA CCACAGAGCT CACCTCTGGA 
CTGGGACGCC TTTCGCTGTC TTCTGAAGAC GACTCTGTGT TTGAGAAAGA CAGGACGTCT 
GCTTTGCGGG CAGCAGAGAA TGATGGAACA GAGAGGAGAA ATGAGGTGGA CGTGAGGTCC 
AGTGCGAGTT CAGATGAGTA CTTTCTGGCC AGTGAGCGGC TGGAACCTAT AAACCAACAG 
ACTATAGATA CTTCAGGGTG CGAGCGCATG ATCTGCGCCA GGTCAAAGTC CTGGGATCAT 
GGGGGAAGAG ACCTCAGCTC CTCGGGGTCT TCCAGCTCCT ACAGATCACT CGACAGCTCT 
CAGGACTTCA TACTGCGGAC TCCTCCAAGA GTGACCAAAG GCCTCTTCAT TGAAGGGGAT 
TCACCCACCA AGTTGGACAG AGAGGTTTTG TCAGCTTTAG GAGTCTCGGA CTTCGACCCT 
CTAAAGTTTC CCAGCATTCA CAAATGGAAG AGCACAATTC AGACATACTC ACAGTCAGAA 
ATGCAAAGCT GGCTGACTCC TGCAGTGCAC AAAAGCCATC TGAGGGCTCT CTCGGCCACT 
CCTGGCTCTC CAGTGAGCGG TTTGATGTCC CCCGTGAGCC GCTTCAGCCC AGCTCGACAC 
ATGGGCTCGC CAGACAACTG CAGTCCCGGA CGCTACAGTC CAGCACACTC CACCTACAGC 
CCCGCTCACC CCAACTACAG CCCTGTCAGC TACATTCAAC GCATCCGCCT CAAACATTTC 
GGAGATACTC CTATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

15000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis.
Genome research13(3)455-66(2003 Mar)
Lo J,Lee S,Xu M,Liu F,Ruan H,Eun A,He Y,Ma W,Wang W,Wen Z,Peng J

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ankle2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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