Ankle2 cDNA ORF clone, Mus musculus(house mouse)

The following Ankle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu05641 NM_001253814.1
Latest version!
Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu04795 NM_027922.2
Latest version!
Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu05641
    Clone ID Related Accession (Same CDS sequence) NM_001253814.1
    Accession Version NM_001253814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Mus musculus(house mouse)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132187.1, AC118260.7, BC065071.1 and CD538245.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK132187.1, SRR7652917.993114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
    GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG
    GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA
    CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT
    GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA
    AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC
    TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG
    ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT
    TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC
    CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT
    AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT
    GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA
    GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA
    TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG
    CCCCTCGGTG GCAGTGATGG GCTGAAAGCA GATGGCTTGT GCCTGGCTGA ATCAGAAACA
    GTAAACAAAG AACGAGCAAA CAGTTACAAA AACCCCCGTA CCCAGGACCT CACGGCCAAG
    CTGCGGAAAG CTGTCGAAAA CGGGGAGGAA CACACCTTTT CTGATCTTAT CTGGAGTAAT
    CCTCGATATC TAATTGGTTC TGGGGACAAC CCAACCATTG TACAAGAAGG ATGTAGGTAC
    AATGTCATGC ATGTTGCTGC CAAAGAGAAC CAGGCTTCCA TGTGCCAGCT GACATTAGAG
    ACTCTGGAGA ACCCTGAGTT TATGCGTCTG ATGTACCCAG ATGACAACAT GGACATGCTA
    CAGAAGCGCA TCCTCTACGT TGTTGACCTG TATCTTAATA CTCCTGACAA AGTGGGCTTT
    GATACACCAC TGCATTTTGC TTGTAAATTT GGAAATGTGG ATGTAGTCAA TGTGCTTTCT
    TCACACCCTT TGATTGTGAA AAACCGAAAG AATAAATATG GTAAAACACC TGAAGAAGTA
    ATTTGTGAAA GAAGCCAAAA CAAATCTCCA GCACTGAAAG AAAGGATTAG AGAATACTTA
    ATGGGTCACT ACTATGTGCC ACTCCTGAGA GCAGAAGACA CTTCTCCTGT CATTGGGGAG
    CTGTGGTCCT CAGACCAGAA AGCTGAAGCC TCAAATACTG CCCACTGTAG AAGCAGCCCC
    AGAGATCCTG TGATGACACT GAGAGCTTTC GTGGGACCTC TGAGTCCATC CAAAGCAGAA
    GATTTTCGAA AGCTCTGGAA AACTCCACCT CGAAAGAAAG CAGGCTTCTT CCACAGCATC
    AGAAAGTCTG ACCCAGAACG AGGCATTGAG AGAGTTGGAA GGGAGCTAGC TCATGAGCTG
    GGGTATCCCT GGGTTGAATA CTGGGAATTT CTGGGATGTT TTGTTGATCT GTCTTCCCAG
    GAAGGCTTGC AAAGACTAGA AGAATACCTT ATCCAGAAGG AGTTAAGCAA AAAGGCTCAA
    CAAGAAATAC GAGAAAATGA AGGCTGCCTT CAGGACAGAA CCTCAGATTT TGGCAGTGGA
    AAGAAGTACA GCAACTCCAT CTCCGTGGGA GCATTTCTAG ATGGGGATGA TGACAGTAGT
    TTGGAAGAAA TTAAAAACCA GCAAAATACA GTTCCAAGTC AGAGTCAGCC CACAACTGAT
    AAGTTCCAAA CCTCTAAAAG TGGTTCCCTT CCCTTGGGAC AGAAGGTCGA CCCTGGAGAA
    ACCTCAGTTG GAACTTACCC AGATAAAGGC AGAAATGGGT TTTGTCACCC TCTAAACCAC
    AGGACTGCAG ATGGAAGGGG ACTAGAGGCC ACCAATGGGG AGGAGGCCCT TCCACCACCT
    GTTTCTGTTT TGACTCAAGA GTTGAATAAA CTGAATTTGC AAAGCCTGGG AGACAGCCTT
    CATGAGACAC CAGATAAAAA CGGGAAACTA GAGGATGAGG TGCTTCCTTC AAGAAAGGGT
    GCAGCAGACA GTGACTTGCT GGCATCTCCT CCTGCCATAG CAAGCCTTGG AAAGAAGCAA
    GTCAGGACCA ACACTGAGGT GTCAGAGGCA ATGGCTGAAA TGTCTTTGGG CCCTAAGAGC
    CCCCAACTTG GAGTTCAGGC TGGTCTGGAA CCTATACTCT CATCTGCCAC GGTAGATTCC
    ACCAAGAGGC TCTTCCTGTC TGGAGAGGAA CCATCAAAAC TAGATCGAGA TGTTTTAGCA
    GCTCTTGAGT GTGCGAATAT TGACCCTGGT CTGTACCCAG CAATCCACAG ATGGAAAAGC
    ACTGTCATGT GCTACTCGCC CTCAGACAGA CAGAGTTGGC CAAGTCCTGC ACTCAAAGGG
    AAGTTCACAA CTGAGTTGGT GGATCTCGAT TGCTCTCATA GCTGCTCAGG ACGATGCAGT
    CCAGCTGGTA GCAGCCCCAG TAAGCCTGGC CACACCTCCT CCTCTTCTGG CCTGCACAGC
    CCTGGACGCT ACAGTCCAGC ACATGGGAGA CACTTCCAAA GAGTGGCACA CGTGGCCCGA
    CTTGCAGCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001240743.1
    CDS234..3128
    Translation

    Target ORF information:

    RefSeq Version NM_001253814.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001253814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
    GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG
    GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA
    CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT
    GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA
    AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC
    TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG
    ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT
    TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC
    CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT
    AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT
    GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA
    GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA
    TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG
    CCCCTCGGTG GCAGTGATGG GCTGAAAGCA GATGGCTTGT GCCTGGCTGA ATCAGAAACA
    GTAAACAAAG AACGAGCAAA CAGTTACAAA AACCCCCGTA CCCAGGACCT CACGGCCAAG
    CTGCGGAAAG CTGTCGAAAA CGGGGAGGAA CACACCTTTT CTGATCTTAT CTGGAGTAAT
    CCTCGATATC TAATTGGTTC TGGGGACAAC CCAACCATTG TACAAGAAGG ATGTAGGTAC
    AATGTCATGC ATGTTGCTGC CAAAGAGAAC CAGGCTTCCA TGTGCCAGCT GACATTAGAG
    ACTCTGGAGA ACCCTGAGTT TATGCGTCTG ATGTACCCAG ATGACAACAT GGACATGCTA
    CAGAAGCGCA TCCTCTACGT TGTTGACCTG TATCTTAATA CTCCTGACAA AGTGGGCTTT
    GATACACCAC TGCATTTTGC TTGTAAATTT GGAAATGTGG ATGTAGTCAA TGTGCTTTCT
    TCACACCCTT TGATTGTGAA AAACCGAAAG AATAAATATG GTAAAACACC TGAAGAAGTA
    ATTTGTGAAA GAAGCCAAAA CAAATCTCCA GCACTGAAAG AAAGGATTAG AGAATACTTA
    ATGGGTCACT ACTATGTGCC ACTCCTGAGA GCAGAAGACA CTTCTCCTGT CATTGGGGAG
    CTGTGGTCCT CAGACCAGAA AGCTGAAGCC TCAAATACTG CCCACTGTAG AAGCAGCCCC
    AGAGATCCTG TGATGACACT GAGAGCTTTC GTGGGACCTC TGAGTCCATC CAAAGCAGAA
    GATTTTCGAA AGCTCTGGAA AACTCCACCT CGAAAGAAAG CAGGCTTCTT CCACAGCATC
    AGAAAGTCTG ACCCAGAACG AGGCATTGAG AGAGTTGGAA GGGAGCTAGC TCATGAGCTG
    GGGTATCCCT GGGTTGAATA CTGGGAATTT CTGGGATGTT TTGTTGATCT GTCTTCCCAG
    GAAGGCTTGC AAAGACTAGA AGAATACCTT ATCCAGAAGG AGTTAAGCAA AAAGGCTCAA
    CAAGAAATAC GAGAAAATGA AGGCTGCCTT CAGGACAGAA CCTCAGATTT TGGCAGTGGA
    AAGAAGTACA GCAACTCCAT CTCCGTGGGA GCATTTCTAG ATGGGGATGA TGACAGTAGT
    TTGGAAGAAA TTAAAAACCA GCAAAATACA GTTCCAAGTC AGAGTCAGCC CACAACTGAT
    AAGTTCCAAA CCTCTAAAAG TGGTTCCCTT CCCTTGGGAC AGAAGGTCGA CCCTGGAGAA
    ACCTCAGTTG GAACTTACCC AGATAAAGGC AGAAATGGGT TTTGTCACCC TCTAAACCAC
    AGGACTGCAG ATGGAAGGGG ACTAGAGGCC ACCAATGGGG AGGAGGCCCT TCCACCACCT
    GTTTCTGTTT TGACTCAAGA GTTGAATAAA CTGAATTTGC AAAGCCTGGG AGACAGCCTT
    CATGAGACAC CAGATAAAAA CGGGAAACTA GAGGATGAGG TGCTTCCTTC AAGAAAGGGT
    GCAGCAGACA GTGACTTGCT GGCATCTCCT CCTGCCATAG CAAGCCTTGG AAAGAAGCAA
    GTCAGGACCA ACACTGAGGT GTCAGAGGCA ATGGCTGAAA TGTCTTTGGG CCCTAAGAGC
    CCCCAACTTG GAGTTCAGGC TGGTCTGGAA CCTATACTCT CATCTGCCAC GGTAGATTCC
    ACCAAGAGGC TCTTCCTGTC TGGAGAGGAA CCATCAAAAC TAGATCGAGA TGTTTTAGCA
    GCTCTTGAGT GTGCGAATAT TGACCCTGGT CTGTACCCAG CAATCCACAG ATGGAAAAGC
    ACTGTCATGT GCTACTCGCC CTCAGACAGA CAGAGTTGGC CAAGTCCTGC ACTCAAAGGG
    AAGTTCACAA CTGAGTTGGT GGATCTCGAT TGCTCTCATA GCTGCTCAGG ACGATGCAGT
    CCAGCTGGTA GCAGCCCCAG TAAGCCTGGC CACACCTCCT CCTCTTCTGG CCTGCACAGC
    CCTGGACGCT ACAGTCCAGC ACATGGGAGA CACTTCCAAA GAGTGGCACA CGTGGCCCGA
    CTTGCAGCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu04795
    Clone ID Related Accession (Same CDS sequence) NM_027922.2
    Accession Version NM_027922.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-05
    Organism Mus musculus(house mouse)
    Product ankyrin repeat and LEM domain-containing protein 2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132187.1, BC065071.1 and CD538245.1. On Dec 7, 2011 this sequence version replaced NM_027922.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC065071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
    GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG
    GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA
    CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT
    GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA
    AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC
    TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG
    ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT
    TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC
    CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT
    AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT
    GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA
    GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA
    TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG
    CCCCTCGGTG GCAGTGATGG GCTGAAAGAT GGCTTGTGCC TGGCTGAATC AGAAACAGTA
    AACAAAGAAC GAGCAAACAG TTACAAAAAC CCCCGTACCC AGGACCTCAC GGCCAAGCTG
    CGGAAAGCTG TCGAAAACGG GGAGGAACAC ACCTTTTCTG ATCTTATCTG GAGTAATCCT
    CGATATCTAA TTGGTTCTGG GGACAACCCA ACCATTGTAC AAGAAGGATG TAGGTACAAT
    GTCATGCATG TTGCTGCCAA AGAGAACCAG GCTTCCATGT GCCAGCTGAC ATTAGAGACT
    CTGGAGAACC CTGAGTTTAT GCGTCTGATG TACCCAGATG ACAACATGGA CATGCTACAG
    AAGCGCATCC TCTACGTTGT TGACCTGTAT CTTAATACTC CTGACAAAGT GGGCTTTGAT
    ACACCACTGC ATTTTGCTTG TAAATTTGGA AATGTGGATG TAGTCAATGT GCTTTCTTCA
    CACCCTTTGA TTGTGAAAAA CCGAAAGAAT AAATATGGTA AAACACCTGA AGAAGTAATT
    TGTGAAAGAA GCCAAAACAA ATCTCCAGCA CTGAAAGAAA GGATTAGAGA ATACTTAATG
    GGTCACTACT ATGTGCCACT CCTGAGAGCA GAAGACACTT CTCCTGTCAT TGGGGAGCTG
    TGGTCCTCAG ACCAGAAAGC TGAAGCCTCA AATACTGCCC ACTGTAGAAG CAGCCCCAGA
    GATCCTGTGA TGACACTGAG AGCTTTCGTG GGACCTCTGA GTCCATCCAA AGCAGAAGAT
    TTTCGAAAGC TCTGGAAAAC TCCACCTCGA AAGAAAGCAG GCTTCTTCCA CAGCATCAGA
    AAGTCTGACC CAGAACGAGG CATTGAGAGA GTTGGAAGGG AGCTAGCTCA TGAGCTGGGG
    TATCCCTGGG TTGAATACTG GGAATTTCTG GGATGTTTTG TTGATCTGTC TTCCCAGGAA
    GGCTTGCAAA GACTAGAAGA ATACCTTATC CAGAAGGAGT TAAGCAAAAA GGCTCAACAA
    GAAATACGAG AAAATGAAGG CTGCCTTCAG GACAGAACCT CAGATTTTGG CAGTGGAAAG
    AAGTACAGCA ACTCCATCTC CGTGGGAGCA TTTCTAGATG GGGATGATGA CAGTAGTTTG
    GAAGAAATTA AAAACCAGCA AAATACAGTT CCAAGTCAGA GTCAGCCCAC AACTGATAAG
    TTCCAAACCT CTAAAAGTGG TTCCCTTCCC TTGGGACAGA AGGTCGACCC TGGAGAAACC
    TCAGTTGGAA CTTACCCAGA TAAAGGCAGA AATGGGTTTT GTCACCCTCT AAACCACAGG
    ACTGCAGATG GAAGGGGACT AGAGGCCACC AATGGGGAGG AGGCCCTTCC ACCACCTGTT
    TCTGTTTTGA CTCAAGAGTT GAATAAACTG AATTTGCAAA GCCTGGGAGA CAGCCTTCAT
    GAGACACCAG ATAAAAACGG GAAACTAGAG GATGAGGTGC TTCCTTCAAG AAAGGGTGCA
    GCAGACAGTG ACTTGCTGGC ATCTCCTCCT GCCATAGCAA GCCTTGGAAA GAAGCAAGTC
    AGGACCAACA CTGAGGTGTC AGAGGCAATG GCTGAAATGT CTTTGGGCCC TAAGAGCCCC
    CAACTTGGAG TTCAGGCTGG TCTGGAACCT ATACTCTCAT CTGCCACGGT AGATTCCACC
    AAGAGGCTCT TCCTGTCTGG AGAGGAACCA TCAAAACTAG ATCGAGATGT TTTAGCAGCT
    CTTGAGTGTG CGAATATTGA CCCTGGTCTG TACCCAGCAA TCCACAGATG GAAAAGCACT
    GTCATGTGCT ACTCGCCCTC AGACAGACAG AGTTGGCCAA GTCCTGCACT CAAAGGGAAG
    TTCACAACTG AGTTGGTGGA TCTCGATTGC TCTCATAGCT GCTCAGGACG ATGCAGTCCA
    GCTGGTAGCA GCCCCAGTAA GCCTGGCCAC ACCTCCTCCT CTTCTGGCCT GCACAGCCCT
    GGACGCTACA GTCCAGCACA TGGGAGACAC TTCCAAAGAG TGGCACACGT GGCCCGACTT
    GCAGCTCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_082198.1
    CDS234..3125
    Translation

    Target ORF information:

    RefSeq Version NM_027922.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus ankyrin repeat and LEM domain containing 2 (Ankle2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_027922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGCTGTGGC AGCGGCTGGC TGTGGTGGAG TGGGCGGCCC TGGCCTGGGA GCTCCTGGGC 
    GCCTCGGTGC TGTTCATCGC CGTGCGCTGG CTGGTCCGCC GGCTAGAGAA GCGGCCGCGG
    GACCTGAACC GTTGCGGGAC GCTCTCCTCT CCGCCCAGCG CGTCTGAAGC GGTGGCCGCA
    CAGCCAGGTG AAGTGACAAT GGATGCCATG ATGGCTCGAC TGAAACTCTT GAATCCAGAT
    GACCTTAGAA AAGAAGTTAT GAAAGCTGGA CTTAAATGTG GACCCATTAC ATCAACAACA
    AGATTTATTT TTGAGAAGAA ATTAGCTCAG GCCTTACTGG AACAAGGTGG ATTGCTGACC
    TCTTCCCTTC CCAAGCCCAG CGCGGTTACT GCCATGGCAT TTATCCAGGG CACCTCAAGG
    ACTCCTCCAT CTGTGGACGG GAAACAAACT CAGCAGGCCT GTTTTTCTGA AGACAGAGAT
    TTTGGTTACA GTGTGGGCTT GAACCCTCCA GAAGAGGAAG CGGTAGCATC CAGTGTCCAC
    CCAGTACCCT TCAGTGCCTC TACCAGGAAT GACAACCACA AGGCTGGAGT GACAGCTCCT
    AAGGAGCCAC TTGTGTACTA TGGAGTGTGT CCAGTATATG AGGATGGCCC TGTGAGACAT
    GAGAGGATCC ATGTTTACGA AGATAAAAAG GAAGCTTTAC AAGCTGCCAA GTTGATCAAA
    GGGTCCCGGT TTAAAGCTTT TCGAACAAGA GAAGATGCTG AGAAATTTGC TAGAGGGATA
    TGTGATTATT TACCTTCTCC CAACAAAACT ACGCCCTTAC TGTCTCCTGT GAAGGCAGTG
    CCCCTCGGTG GCAGTGATGG GCTGAAAGAT GGCTTGTGCC TGGCTGAATC AGAAACAGTA
    AACAAAGAAC GAGCAAACAG TTACAAAAAC CCCCGTACCC AGGACCTCAC GGCCAAGCTG
    CGGAAAGCTG TCGAAAACGG GGAGGAACAC ACCTTTTCTG ATCTTATCTG GAGTAATCCT
    CGATATCTAA TTGGTTCTGG GGACAACCCA ACCATTGTAC AAGAAGGATG TAGGTACAAT
    GTCATGCATG TTGCTGCCAA AGAGAACCAG GCTTCCATGT GCCAGCTGAC ATTAGAGACT
    CTGGAGAACC CTGAGTTTAT GCGTCTGATG TACCCAGATG ACAACATGGA CATGCTACAG
    AAGCGCATCC TCTACGTTGT TGACCTGTAT CTTAATACTC CTGACAAAGT GGGCTTTGAT
    ACACCACTGC ATTTTGCTTG TAAATTTGGA AATGTGGATG TAGTCAATGT GCTTTCTTCA
    CACCCTTTGA TTGTGAAAAA CCGAAAGAAT AAATATGGTA AAACACCTGA AGAAGTAATT
    TGTGAAAGAA GCCAAAACAA ATCTCCAGCA CTGAAAGAAA GGATTAGAGA ATACTTAATG
    GGTCACTACT ATGTGCCACT CCTGAGAGCA GAAGACACTT CTCCTGTCAT TGGGGAGCTG
    TGGTCCTCAG ACCAGAAAGC TGAAGCCTCA AATACTGCCC ACTGTAGAAG CAGCCCCAGA
    GATCCTGTGA TGACACTGAG AGCTTTCGTG GGACCTCTGA GTCCATCCAA AGCAGAAGAT
    TTTCGAAAGC TCTGGAAAAC TCCACCTCGA AAGAAAGCAG GCTTCTTCCA CAGCATCAGA
    AAGTCTGACC CAGAACGAGG CATTGAGAGA GTTGGAAGGG AGCTAGCTCA TGAGCTGGGG
    TATCCCTGGG TTGAATACTG GGAATTTCTG GGATGTTTTG TTGATCTGTC TTCCCAGGAA
    GGCTTGCAAA GACTAGAAGA ATACCTTATC CAGAAGGAGT TAAGCAAAAA GGCTCAACAA
    GAAATACGAG AAAATGAAGG CTGCCTTCAG GACAGAACCT CAGATTTTGG CAGTGGAAAG
    AAGTACAGCA ACTCCATCTC CGTGGGAGCA TTTCTAGATG GGGATGATGA CAGTAGTTTG
    GAAGAAATTA AAAACCAGCA AAATACAGTT CCAAGTCAGA GTCAGCCCAC AACTGATAAG
    TTCCAAACCT CTAAAAGTGG TTCCCTTCCC TTGGGACAGA AGGTCGACCC TGGAGAAACC
    TCAGTTGGAA CTTACCCAGA TAAAGGCAGA AATGGGTTTT GTCACCCTCT AAACCACAGG
    ACTGCAGATG GAAGGGGACT AGAGGCCACC AATGGGGAGG AGGCCCTTCC ACCACCTGTT
    TCTGTTTTGA CTCAAGAGTT GAATAAACTG AATTTGCAAA GCCTGGGAGA CAGCCTTCAT
    GAGACACCAG ATAAAAACGG GAAACTAGAG GATGAGGTGC TTCCTTCAAG AAAGGGTGCA
    GCAGACAGTG ACTTGCTGGC ATCTCCTCCT GCCATAGCAA GCCTTGGAAA GAAGCAAGTC
    AGGACCAACA CTGAGGTGTC AGAGGCAATG GCTGAAATGT CTTTGGGCCC TAAGAGCCCC
    CAACTTGGAG TTCAGGCTGG TCTGGAACCT ATACTCTCAT CTGCCACGGT AGATTCCACC
    AAGAGGCTCT TCCTGTCTGG AGAGGAACCA TCAAAACTAG ATCGAGATGT TTTAGCAGCT
    CTTGAGTGTG CGAATATTGA CCCTGGTCTG TACCCAGCAA TCCACAGATG GAAAAGCACT
    GTCATGTGCT ACTCGCCCTC AGACAGACAG AGTTGGCCAA GTCCTGCACT CAAAGGGAAG
    TTCACAACTG AGTTGGTGGA TCTCGATTGC TCTCATAGCT GCTCAGGACG ATGCAGTCCA
    GCTGGTAGCA GCCCCAGTAA GCCTGGCCAC ACCTCCTCCT CTTCTGGCCT GCACAGCCCT
    GGACGCTACA GTCCAGCACA TGGGAGACAC TTCCAAAGAG TGGCACACGT GGCCCGACTT
    GCAGCTCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.