ankle2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ankle2
Entrez Gene ID	100380184
Full Name	ankyrin repeat and LEM domain containing 2
Synonyms	lemd7
General protein information	Preferred Names ankyrin repeat and LEM domain containing 2 Names ankyrin repeat and LEM domain-containing protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002931879.5	XP_002931925.1	ankyrin repeat and LEM domain-containing protein 2 isoform X3
XM_002931879.4	XP_002931925.1	ankyrin repeat and LEM domain-containing protein 2 isoform X3
XM_004910572.2	XP_004910629.1	ankyrin repeat and LEM domain-containing protein 2 isoform X1
XM_012969394.2	XP_012824848.1	ankyrin repeat and LEM domain-containing protein 2 isoform X2
XM_004910572.3	XP_004910629.2	ankyrin repeat and LEM domain-containing protein 2 isoform X1
XM_012969394.3	XP_012824848.1	ankyrin repeat and LEM domain-containing protein 2 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ankle2	NP_001041366.1
Homo sapiens (human)	ANKLE2	NP_055929.1
Bos taurus (cattle)	ANKLE2	XP_002694494.2
	ANKLE2	XP_001087028.1
Danio rerio (zebrafish)	ankle2	NP_001119867.1
Xenopus tropicalis (tropical clawed frog)	ankle2	XP_004910629.1
Canis lupus familiaris (dog)	ANKLE2	XP_852228.2
Pan troglodytes (chimpanzee)	ANKLE2	XP_001152324.2
Mus musculus (house mouse)	Ankle2	NP_001240743.1
Gallus gallus (chicken)	ANKLE2	XP_415084.4

Gene Ontology

The following ankle2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ankle2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03603	XM_002931879.4 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa33155	XM_012969394.2 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa48941	XM_004910572.3 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa48942	XM_012969394.3 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa48943	XM_002931879.5 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa03602	XM_004910572.2 Latest version!	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ankle2 ( XM_002931879.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931925.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa03603
Clone ID Related Accession (Same CDS sequence)	XM_002931879.4
Accession Version	XM_002931879.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931879.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAATA TCCTTAGCCA GCTGAAGCTC CTCAACCCAG ACCAGCTTAG AGAAGAAATA 
TTAAAGGCTG GATTAAAGTG TGGACCAATC ACATCCACCA CAAGATTTAT ATTTGAGAAG 
AAACTAGCAC GTGTGTTACT GGAGCAACAG GGAGTGAACT TAGAGGAAAC CACAACTGTT 
TGTGATGGGA ATGCAGGTGC GTCTGGGAAT GCAGGCACTG ATAACAGGCA GCACACAAAT 
TACACCCAGA AGAAAAACTG CTTGGAAGAT GGGGACTTTG GGTACAGTGT TGGATTAAAT 
CCACCTTCCG AAGAAGCTCC GTTGGCAGGG AGAGGTTTTT CTGAAGTTAA TGGAAGATCG 
TTAGATGATT GTCAGACCTT CACCCCAACA TGTAAGGATC CATCAATGTT CTATGCTGTT 
TGCCCTGTCT ATGAGGAGAC ACTAAATAAA AATGAAAAAG TACATATTTA TGCAGACAAG 
AAAGAAGCAC TTCAAGTGGT TAAAATGTTA AAAGGTTCAA GGTTTAAGGC TTTTGTCTCC 
AGGGAGGATG CTGAGAGGTT TGCAAGAGGC ATATGTGACT ACTGCCCATC ACCTAGCAAG 
TCTTCAGTGA TCCTGTCACC TGTAAAACTT TCACCAGCTC TAGTGAGAGA TGGAATCAAC 
TCTTCAGAGA TGGAAAACAT CAACAAAGAA AGGGCAAACA ACTTCAAAAG TCCAAGGACA 
CAAGATCTAA CTGCAAAGCT GAGGAAGGCT GTAGAGAAAG GAGATCAAAC AACATTTTCA 
GATCTTATTT GGACCAATCC ACGTTATCTC ATAGGTTCTG GAGATAACCC TACTGTAGTG 
CAGGAAGGGT GCAGGTATAA TGTCATGCAC GTGGCTGCCA AGGAGAATCA GGCTGGTATC 
TGCCAACTCC TCTTAGATAC ATTGGAAAAT CCAGAATTCA TGCGGCTCAT GTACCCAGAT 
GATGATGACC TCATGCTACA TAAGCGGATC CGATATATTG TTGATCTGTA TCTAAACACT 
CCAGATAAAA TGGTTTTTGA CACTCCTTTG CACTTTGCTT GCAAGTTTGG GAATGCTGAT 
GTTGTCAGTG TGCTATGCTC TCATCCTGAC ATTTTAAAAA ATCCTCGAAA CAAGTATGAA 
CAGACCCCGG AAGAGGCTGT CTGTGAAAGA AGCAAAAATA AATCTGCAGA ACTTAAGAAT 
AAGATAAAAG AGTATTTAAA AGGTCATTTT TATGTGCCAC TGTTACGGGC TGAGGATAAC 
TCCTCGTTTC CAGTTATAGG AGCTCCATGG TCTCCCGAGC AGCCTGACCT AATCCAGACC 
AGATTCTCTG GCAGTCCCAA AGATCCGCTA TTAGCTGTGA GAGCATTTGC AGGCCCTATG 
AGCCCTTCTA AGGCAGAAGA TTTCAGAAGA GTCTGGAAAA CACCACCACG GGAGAAAGCT 
GAATTTTATC ACAAAATCAG AAAAACAGAC CCAGAAAGAG GAGCAGAGAG AGTGGGAAGA 
GTGCTCGCTC ATGAACTGGA TGTGCCTTGG GTGGAGTACT GGGATTTCCT TGGCTGCTTT 
GTTGACCTCT CTTCCCAGGA GGGTCTGAGG AAGCTGGAGG AATATCTGAG CAAGAGAGAA 
ACAAGTGAAA GGGCACAGCG GGATTCAGAT TATGAAATCT GTAACAAGTA TAAAACGCCT 
TCAGGAAAGA GCAAAAAAAT GTGCAATTCC ATTTCTGTTG GAGCCTTTCT GGATGATGAG 
GATGAAATGA GTTTGGAAGA GCTAAAAAAC CGCCAAAATG CAGCGCTAAA ACACAGCTAC 
TCTCACTCAG AGACAATCAC GGTGCCTGAG TGTATTATGG AAACCAACAC AGTTCCTAGT 
GGTGAGCACC AAAGTGGTAC CAAACCTGGA TTCTGTAGCC CATTATGCCA TGATCAGTAT 
CTTCCTGGGG AAAGTAGGCA TAAAGGTGTC CATGAAGGAA TGCTATCACC AGTCTCTAAC 
CTTATGGCTG AATTTGATAA ACTGGCAATT TCAGAAAAAA GTGAGCTTGA GAATGAGACA 
TGGAACATAG AGCCTTCTCT AATAGCTAAA GAAACTGAGC ACAATTTACA TGCTGGTTCA 
TCAACCAGGA CAAATGGTTC AGTGTTATCA CAAGTTGAGC AAATGATGCC AGAGCTCAGC 
CTGCAGGATA AACTTTCTCC ACCAAGAAAC AGTATTGGTC TCTCACCCCC ACGTGCCTCT 
TTGGACTCTT CCATTCATCT GGGTATGAAG ACTCCACCAA GAAACCAATG CAACAAAAGC 
ATCTTCCTGC TGGGAAGGGA ACCTTCCAAA CTAGACAGTG ATGTTCTGTC AGCAGTGGAA 
AATGTAGAAC TCGATGGACA TCAGTACCCC AGTGTTTCTA AATGGATGCA AGCAGTGTTG 
TCTTATCCCA ATACAGCTCG CCAAAGTTGG CCAAGTCCGG CACTTCTGTC AGGTAGAGGT 
AAGTCTCAGA TGTTTTGTTC CAATTCACCT GGAAGCCATG CCTATTCTAC GCCTGGGCGA 
AACAGTCCCA TTCCTGGAAG CCCTGGGAAA TACATGAACA CATCTGATTA TGGCAGCCCA 
GGAAGGTATA GCCCTGCATA CGCCAGTCAT ATCCAATTGC TACGTGTCAG ACACTTTTCA 
GACCACCCTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931925.1
CDS	344..3001
Translation	MDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_002931879.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931879.4

ORF Insert Sequence:

ATGGATAATA TCCTTAGCCA GCTGAAGCTC CTCAACCCAG ACCAGCTTAG AGAAGAAATA 
TTAAAGGCTG GATTAAAGTG TGGACCAATC ACATCCACCA CAAGATTTAT ATTTGAGAAG 
AAACTAGCAC GTGTGTTACT GGAGCAACAG GGAGTGAACT TAGAGGAAAC CACAACTGTT 
TGTGATGGGA ATGCAGGTGC GTCTGGGAAT GCAGGCACTG ATAACAGGCA GCACACAAAT 
TACACCCAGA AGAAAAACTG CTTGGAAGAT GGGGACTTTG GGTACAGTGT TGGATTAAAT 
CCACCTTCCG AAGAAGCTCC GTTGGCAGGG AGAGGTTTTT CTGAAGTTAA TGGAAGATCG 
TTAGATGATT GTCAGACCTT CACCCCAACA TGTAAGGATC CATCAATGTT CTATGCTGTT 
TGCCCTGTCT ATGAGGAGAC ACTAAATAAA AATGAAAAAG TACATATTTA TGCAGACAAG 
AAAGAAGCAC TTCAAGTGGT TAAAATGTTA AAAGGTTCAA GGTTTAAGGC TTTTGTCTCC 
AGGGAGGATG CTGAGAGGTT TGCAAGAGGC ATATGTGACT ACTGCCCATC ACCTAGCAAG 
TCTTCAGTGA TCCTGTCACC TGTAAAACTT TCACCAGCTC TAGTGAGAGA TGGAATCAAC 
TCTTCAGAGA TGGAAAACAT CAACAAAGAA AGGGCAAACA ACTTCAAAAG TCCAAGGACA 
CAAGATCTAA CTGCAAAGCT GAGGAAGGCT GTAGAGAAAG GAGATCAAAC AACATTTTCA 
GATCTTATTT GGACCAATCC ACGTTATCTC ATAGGTTCTG GAGATAACCC TACTGTAGTG 
CAGGAAGGGT GCAGGTATAA TGTCATGCAC GTGGCTGCCA AGGAGAATCA GGCTGGTATC 
TGCCAACTCC TCTTAGATAC ATTGGAAAAT CCAGAATTCA TGCGGCTCAT GTACCCAGAT 
GATGATGACC TCATGCTACA TAAGCGGATC CGATATATTG TTGATCTGTA TCTAAACACT 
CCAGATAAAA TGGTTTTTGA CACTCCTTTG CACTTTGCTT GCAAGTTTGG GAATGCTGAT 
GTTGTCAGTG TGCTATGCTC TCATCCTGAC ATTTTAAAAA ATCCTCGAAA CAAGTATGAA 
CAGACCCCGG AAGAGGCTGT CTGTGAAAGA AGCAAAAATA AATCTGCAGA ACTTAAGAAT 
AAGATAAAAG AGTATTTAAA AGGTCATTTT TATGTGCCAC TGTTACGGGC TGAGGATAAC 
TCCTCGTTTC CAGTTATAGG AGCTCCATGG TCTCCCGAGC AGCCTGACCT AATCCAGACC 
AGATTCTCTG GCAGTCCCAA AGATCCGCTA TTAGCTGTGA GAGCATTTGC AGGCCCTATG 
AGCCCTTCTA AGGCAGAAGA TTTCAGAAGA GTCTGGAAAA CACCACCACG GGAGAAAGCT 
GAATTTTATC ACAAAATCAG AAAAACAGAC CCAGAAAGAG GAGCAGAGAG AGTGGGAAGA 
GTGCTCGCTC ATGAACTGGA TGTGCCTTGG GTGGAGTACT GGGATTTCCT TGGCTGCTTT 
GTTGACCTCT CTTCCCAGGA GGGTCTGAGG AAGCTGGAGG AATATCTGAG CAAGAGAGAA 
ACAAGTGAAA GGGCACAGCG GGATTCAGAT TATGAAATCT GTAACAAGTA TAAAACGCCT 
TCAGGAAAGA GCAAAAAAAT GTGCAATTCC ATTTCTGTTG GAGCCTTTCT GGATGATGAG 
GATGAAATGA GTTTGGAAGA GCTAAAAAAC CGCCAAAATG CAGCGCTAAA ACACAGCTAC 
TCTCACTCAG AGACAATCAC GGTGCCTGAG TGTATTATGG AAACCAACAC AGTTCCTAGT 
GGTGAGCACC AAAGTGGTAC CAAACCTGGA TTCTGTAGCC CATTATGCCA TGATCAGTAT 
CTTCCTGGGG AAAGTAGGCA TAAAGGTGTC CATGAAGGAA TGCTATCACC AGTCTCTAAC 
CTTATGGCTG AATTTGATAA ACTGGCAATT TCAGAAAAAA GTGAGCTTGA GAATGAGACA 
TGGAACATAG AGCCTTCTCT AATAGCTAAA GAAACTGAGC ACAATTTACA TGCTGGTTCA 
TCAACCAGGA CAAATGGTTC AGTGTTATCA CAAGTTGAGC AAATGATGCC AGAGCTCAGC 
CTGCAGGATA AACTTTCTCC ACCAAGAAAC AGTATTGGTC TCTCACCCCC ACGTGCCTCT 
TTGGACTCTT CCATTCATCT GGGTATGAAG ACTCCACCAA GAAACCAATG CAACAAAAGC 
ATCTTCCTGC TGGGAAGGGA ACCTTCCAAA CTAGACAGTG ATGTTCTGTC AGCAGTGGAA 
AATGTAGAAC TCGATGGACA TCAGTACCCC AGTGTTTCTA AATGGATGCA AGCAGTGTTG 
TCTTATCCCA ATACAGCTCG CCAAAGTTGG CCAAGTCCGG CACTTCTGTC AGGTAGAGGT 
AAGTCTCAGA TGTTTTGTTC CAATTCACCT GGAAGCCATG CCTATTCTAC GCCTGGGCGA 
AACAGTCCCA TTCCTGGAAG CCCTGGGAAA TACATGAACA CATCTGATTA TGGCAGCCCA 
GGAAGGTATA GCCCTGCATA CGCCAGTCAT ATCCAATTGC TACGTGTCAG ACACTTTTCA 
GACCACCCTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_012969394.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824848.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa33155
Clone ID Related Accession (Same CDS sequence)	XM_012969394.2
Accession Version	XM_012969394.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GTATGGATAA TATCCTTAGC CAGCTGAAGC TCCTCAACCC AGACCAGCTT 
AGAGAAGAAA TATTAAAGGC TGGATTAAAG TGTGGACCAA TCACATCCAC CACAAGATTT 
ATATTTGAGA AGAAACTAGC ACGTGTGTTA CTGGAGCAAC AGGGAGTGAA CTTAGAGGAA 
ACCACAACTG TTTGTGATGG GAATGCAGGT GCGTCTGGGA ATGCAGGCAC TGATAACAGG 
CAGCACACAA ATTACACCCA GAAGAAAAAC TGCTTGGAAG ATGGGGACTT TGGGTACAGT 
GTTGGATTAA ATCCACCTTC CGAAGAAGCT CCGTTGGCAG GGAGAGGTTT TTCTGAAGTT 
AATGGAAGAT CGTTAGATGA TTGTCAGACC TTCACCCCAA CATGTAAGGA TCCATCAATG 
TTCTATGCTG TTTGCCCTGT CTATGAGGAG ACACTAAATA AAAATGAAAA AGTACATATT 
TATGCAGACA AGAAAGAAGC ACTTCAAGTG GTTAAAATGT TAAAAGGTTC AAGGTTTAAG 
GCTTTTGTCT CCAGGGAGGA TGCTGAGAGG TTTGCAAGAG GCATATGTGA CTACTGCCCA 
TCACCTAGCA AGTCTTCAGT GATCCTGTCA CCTGTAAAAC TTTCACCAGC TCTAGTGAGA 
GATGGAATCA ACTCTTCAGA GATGGAAAAC ATCAACAAAG AAAGGGCAAA CAACTTCAAA 
AGTCCAAGGA CACAAGATCT AACTGCAAAG CTGAGGAAGG CTGTAGAGAA AGGAGATCAA 
ACAACATTTT CAGATCTTAT TTGGACCAAT CCACGTTATC TCATAGGTTC TGGAGATAAC 
CCTACTGTAG TGCAGGAAGG GTGCAGGTAT AATGTCATGC ACGTGGCTGC CAAGGAGAAT 
CAGGCTGGTA TCTGCCAACT CCTCTTAGAT ACATTGGAAA ATCCAGAATT CATGCGGCTC 
ATGTACCCAG ATGATGATGA CCTCATGCTA CATAAGCGGA TCCGATATAT TGTTGATCTG 
TATCTAAACA CTCCAGATAA AATGGTTTTT GACACTCCTT TGCACTTTGC TTGCAAGTTT 
GGGAATGCTG ATGTTGTCAG TGTGCTATGC TCTCATCCTG ACATTTTAAA AAATCCTCGA 
AACAAGTATG AACAGACCCC GGAAGAGGCT GTCTGTGAAA GAAGCAAAAA TAAATCTGCA 
GAACTTAAGA ATAAGATAAA AGAGTATTTA AAAGGTCATT TTTATGTGCC ACTGTTACGG 
GCTGAGGATA ACTCCTCGTT TCCAGTTATA GGAGCTCCAT GGTCTCCCGA GCAGCCTGAC 
CTAATCCAGA CCAGATTCTC TGGCAGTCCC AAAGATCCGC TATTAGCTGT GAGAGCATTT 
GCAGGCCCTA TGAGCCCTTC TAAGGCAGAA GATTTCAGAA GAGTCTGGAA AACACCACCA 
CGGGAGAAAG CTGAATTTTA TCACAAAATC AGAAAAACAG ACCCAGAAAG AGGAGCAGAG 
AGAGTGGGAA GAGTGCTCGC TCATGAACTG GATGTGCCTT GGGTGGAGTA CTGGGATTTC 
CTTGGCTGCT TTGTTGACCT CTCTTCCCAG GAGGGTCTGA GGAAGCTGGA GGAATATCTG 
AGCAAGAGAG AAACAAGTGA AAGGGCACAG CGGGATTCAG ATTATGAAAT CTGTAACAAG 
TATAAAACGC CTTCAGGAAA GAGCAAAAAA ATGTGCAATT CCATTTCTGT TGGAGCCTTT 
CTGGATGATG AGGATGAAAT GAGTTTGGAA GAGCTAAAAA ACCGCCAAAA TGCAGCGCTA 
AAACACAGCT ACTCTCACTC AGAGACAATC ACGGTGCCTG AGTGTATTAT GGAAACCAAC 
ACAGTTCCTA GTGGTGAGCA CCAAAGTGGT ACCAAACCTG GATTCTGTAG CCCATTATGC 
CATGATCAGT ATCTTCCTGG GGAAAGTAGG CATAAAGGTG TCCATGAAGG AATGCTATCA 
CCAGTCTCTA ACCTTATGGC TGAATTTGAT AAACTGGCAA TTTCAGAAAA AAGTGAGCTT 
GAGAATGAGA CATGGAACAT AGAGCCTTCT CTAATAGCTA AAGAAACTGA GCACAATTTA 
CATGCTGGTT CATCAACCAG GACAAATGGT TCAGTGTTAT CACAAGTTGA GCAAATGATG 
CCAGAGCTCA GCCTGCAGGA TAAACTTTCT CCACCAAGAA ACAGTATTGG TCTCTCACCC 
CCACGTGCCT CTTTGGACTC TTCCATTCAT CTGGGTATGA AGACTCCACC AAGAAACCAA 
TGCAACAAAA GCATCTTCCT GCTGGGAAGG GAACCTTCCA AACTAGACAG TGATGTTCTG 
TCAGCAGTGG AAAATGTAGA ACTCGATGGA CATCAGTACC CCAGTGTTTC TAAATGGATG 
CAAGCAGTGT TGTCTTATCC CAATACAGCT CGCCAAAGTT GGCCAAGTCC GGCACTTCTG 
TCAGGTAGAG GTAAGTCTCA GATGTTTTGT TCCAATTCAC CTGGAAGCCA TGCCTATTCT 
ACGCCTGGGC GAAACAGTCC CATTCCTGGA AGCCCTGGGA AATACATGAA CACATCTGAT 
TATGGCAGCC CAGGAAGGTA TAGCCCTGCA TACGCCAGTC ATATCCAATT GCTACGTGTC 
AGACACTTTT CAGACCACCC TGCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824848.1
CDS	85..2754
Translation	MAQSMDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_012969394.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969394.2

ORF Insert Sequence:

ATGGCACAGA GTATGGATAA TATCCTTAGC CAGCTGAAGC TCCTCAACCC AGACCAGCTT 
AGAGAAGAAA TATTAAAGGC TGGATTAAAG TGTGGACCAA TCACATCCAC CACAAGATTT 
ATATTTGAGA AGAAACTAGC ACGTGTGTTA CTGGAGCAAC AGGGAGTGAA CTTAGAGGAA 
ACCACAACTG TTTGTGATGG GAATGCAGGT GCGTCTGGGA ATGCAGGCAC TGATAACAGG 
CAGCACACAA ATTACACCCA GAAGAAAAAC TGCTTGGAAG ATGGGGACTT TGGGTACAGT 
GTTGGATTAA ATCCACCTTC CGAAGAAGCT CCGTTGGCAG GGAGAGGTTT TTCTGAAGTT 
AATGGAAGAT CGTTAGATGA TTGTCAGACC TTCACCCCAA CATGTAAGGA TCCATCAATG 
TTCTATGCTG TTTGCCCTGT CTATGAGGAG ACACTAAATA AAAATGAAAA AGTACATATT 
TATGCAGACA AGAAAGAAGC ACTTCAAGTG GTTAAAATGT TAAAAGGTTC AAGGTTTAAG 
GCTTTTGTCT CCAGGGAGGA TGCTGAGAGG TTTGCAAGAG GCATATGTGA CTACTGCCCA 
TCACCTAGCA AGTCTTCAGT GATCCTGTCA CCTGTAAAAC TTTCACCAGC TCTAGTGAGA 
GATGGAATCA ACTCTTCAGA GATGGAAAAC ATCAACAAAG AAAGGGCAAA CAACTTCAAA 
AGTCCAAGGA CACAAGATCT AACTGCAAAG CTGAGGAAGG CTGTAGAGAA AGGAGATCAA 
ACAACATTTT CAGATCTTAT TTGGACCAAT CCACGTTATC TCATAGGTTC TGGAGATAAC 
CCTACTGTAG TGCAGGAAGG GTGCAGGTAT AATGTCATGC ACGTGGCTGC CAAGGAGAAT 
CAGGCTGGTA TCTGCCAACT CCTCTTAGAT ACATTGGAAA ATCCAGAATT CATGCGGCTC 
ATGTACCCAG ATGATGATGA CCTCATGCTA CATAAGCGGA TCCGATATAT TGTTGATCTG 
TATCTAAACA CTCCAGATAA AATGGTTTTT GACACTCCTT TGCACTTTGC TTGCAAGTTT 
GGGAATGCTG ATGTTGTCAG TGTGCTATGC TCTCATCCTG ACATTTTAAA AAATCCTCGA 
AACAAGTATG AACAGACCCC GGAAGAGGCT GTCTGTGAAA GAAGCAAAAA TAAATCTGCA 
GAACTTAAGA ATAAGATAAA AGAGTATTTA AAAGGTCATT TTTATGTGCC ACTGTTACGG 
GCTGAGGATA ACTCCTCGTT TCCAGTTATA GGAGCTCCAT GGTCTCCCGA GCAGCCTGAC 
CTAATCCAGA CCAGATTCTC TGGCAGTCCC AAAGATCCGC TATTAGCTGT GAGAGCATTT 
GCAGGCCCTA TGAGCCCTTC TAAGGCAGAA GATTTCAGAA GAGTCTGGAA AACACCACCA 
CGGGAGAAAG CTGAATTTTA TCACAAAATC AGAAAAACAG ACCCAGAAAG AGGAGCAGAG 
AGAGTGGGAA GAGTGCTCGC TCATGAACTG GATGTGCCTT GGGTGGAGTA CTGGGATTTC 
CTTGGCTGCT TTGTTGACCT CTCTTCCCAG GAGGGTCTGA GGAAGCTGGA GGAATATCTG 
AGCAAGAGAG AAACAAGTGA AAGGGCACAG CGGGATTCAG ATTATGAAAT CTGTAACAAG 
TATAAAACGC CTTCAGGAAA GAGCAAAAAA ATGTGCAATT CCATTTCTGT TGGAGCCTTT 
CTGGATGATG AGGATGAAAT GAGTTTGGAA GAGCTAAAAA ACCGCCAAAA TGCAGCGCTA 
AAACACAGCT ACTCTCACTC AGAGACAATC ACGGTGCCTG AGTGTATTAT GGAAACCAAC 
ACAGTTCCTA GTGGTGAGCA CCAAAGTGGT ACCAAACCTG GATTCTGTAG CCCATTATGC 
CATGATCAGT ATCTTCCTGG GGAAAGTAGG CATAAAGGTG TCCATGAAGG AATGCTATCA 
CCAGTCTCTA ACCTTATGGC TGAATTTGAT AAACTGGCAA TTTCAGAAAA AAGTGAGCTT 
GAGAATGAGA CATGGAACAT AGAGCCTTCT CTAATAGCTA AAGAAACTGA GCACAATTTA 
CATGCTGGTT CATCAACCAG GACAAATGGT TCAGTGTTAT CACAAGTTGA GCAAATGATG 
CCAGAGCTCA GCCTGCAGGA TAAACTTTCT CCACCAAGAA ACAGTATTGG TCTCTCACCC 
CCACGTGCCT CTTTGGACTC TTCCATTCAT CTGGGTATGA AGACTCCACC AAGAAACCAA 
TGCAACAAAA GCATCTTCCT GCTGGGAAGG GAACCTTCCA AACTAGACAG TGATGTTCTG 
TCAGCAGTGG AAAATGTAGA ACTCGATGGA CATCAGTACC CCAGTGTTTC TAAATGGATG 
CAAGCAGTGT TGTCTTATCC CAATACAGCT CGCCAAAGTT GGCCAAGTCC GGCACTTCTG 
TCAGGTAGAG GTAAGTCTCA GATGTTTTGT TCCAATTCAC CTGGAAGCCA TGCCTATTCT 
ACGCCTGGGC GAAACAGTCC CATTCCTGGA AGCCCTGGGA AATACATGAA CACATCTGAT 
TATGGCAGCC CAGGAAGGTA TAGCCCTGCA TACGCCAGTC ATATCCAATT GCTACGTGTC 
AGACACTTTT CAGACCACCC TGCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_004910572.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910629.2 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48941
Clone ID Related Accession (Same CDS sequence)	XM_004910572.3
Accession Version	XM_004910572.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910572.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA CCCACTTCTC CTGGGATAGT GTGGGGGGCT GCCAGGTCCT GCTGGCCTGT 
GTACTGGTGC TGCTTGGCAC TTGGCTTTTC CTCCTGGCAG AAAGGAGGCG TAGGCACAAG 
TCTAACCCGG AGCCCTGTCT GTACCCCCCG GAGCCCTGTC TGTACCCCCC GGAGCCCTGT 
CTGTACCCCC CGGAGCCCTG TCTGTACCCC CCTGAGCCCT GTCTGTACCC CCCGGAGCCC 
TGTCTGTACC CCCCGGAGCC CTGTCTGTAC CCCCCTGAGC CCTGTCTGTC CGCCCCTCGG 
CCTGCTACCG AAGTGCAATT AGTGGCACAG AGTATGGATA ATATCCTTAG CCAGCTGAAG 
CTCCTCAACC CAGACCAGCT TAGAGAAGAA ATATTAAAGG CTGGATTAAA GTGTGGACCA 
ATCACATCCA CCACAAGATT TATATTTGAG AAGAAACTAG CACGTGTGTT ACTGGAGCAA 
CAGGGAGTGA ACTTAGAGGA AACCACAACT GTTTGTGATG GGAATGCAGG TGCGTCTGGG 
AATGCAGGCA CTGATAACAG GCAGCACACA AATTACACCC AGAAGAAAAA CTGCTTGGAA 
GATGGGGACT TTGGGTACAG TGTTGGATTA AATCCACCTT CCGAAGAAGC TCCGTTGGCA 
GGGAGAGGTT TTTCTGAAGT TAATGGAAGA TCGTTAGATG ATTGTCAGAC CTTCACCCCA 
ACATGTAAGG ATCCATCAAT GTTCTATGCT GTTTGCCCTG TCTATGAGGA GACACTAAAT 
AAAAATGAAA AAGTACATAT TTATGCAGAC AAGAAAGAAG CACTTCAAGT GGTTAAAATG 
TTAAAAGGTT CAAGGTTTAA GGCTTTTGTC TCCAGGGAGG ATGCTGAGAG GTTTGCAAGA 
GGCATATGTG ACTACTGCCC ATCACCTAGC AAGTCTTCAG TGATCCTGTC ACCTGTAAAA 
CTTTCACCAG CTCTAGTGAG AGATGGAATC AACTCTTCAG AGATGGAAAA CATCAACAAA 
GAAAGGGCAA ACAACTTCAA AAGTCCAAGG ACACAAGATC TAACTGCAAA GCTGAGGAAG 
GCTGTAGAGA AAGGAGATCA AACAACATTT TCAGATCTTA TTTGGACCAA TCCACGTTAT 
CTCATAGGTT CTGGAGATAA CCCTACTGTA GTGCAGGAAG GGTGCAGGTA TAATGTCATG 
CACGTGGCTG CCAAGGAGAA TCAGGCTGGT ATCTGCCAAC TCCTCTTAGA TACATTGGAA 
AATCCAGAAT TCATGCGGCT CATGTACCCA GATGATGATG ACCTCATGCT ACATAAGCGG 
ATCCGATATA TTGTTGATCT GTATCTAAAC ACTCCAGATA AAATGGTTTT TGACACTCCT 
TTGCACTTTG CTTGCAAGTT TGGGAATGCT GATGTTGTCA GTGTGCTATG CTCTCATCCT 
GACATTTTAA AAAATCCTCG AAACAAGTAT GAACAGACCC CGGAAGAGGC TGTCTGTGAA 
AGAAGCAAAA ATAAATCTGC AGAACTTAAG AATAAGATAA AAGAGTATTT AAAAGGTCAT 
TTTTATGTGC CACTGTTACG GGCTGAGGAT AACTCCTCGT TTCCAGTTAT AGGAGCTCCA 
TGGTCTCCCG AGCAGCCTGA CCTAATCCAG ACCAGATTCT CTGGCAGTCC CAAAGATCCG 
CTATTAGCTG TGAGAGCATT TGCAGGCCCT ATGAGCCCTT CTAAGGCAGA AGATTTCAGA 
AGAGTCTGGA AAACACCACC ACGGGAGAAA GCTGAATTTT ATCACAAAAT CAGAAAAACA 
GACCCAGAAA GAGGAGCAGA GAGAGTGGGA AGAGTGCTCG CTCATGAACT GGATGTGCCT 
TGGGTGGAGT ACTGGGATTT CCTTGGCTGC TTTGTTGACC TCTCTTCCCA GGAGGGTCTG 
AGGAAGCTGG AGGAATATCT GAGCAAGAGA GAAACAAGTG AAAGGGCACA GCGGGATTCA 
GATTATGAAA TATGTAACAA GTATAAAACG CCTTCAGGAA AGAGCAAAAA AATGTGCAAT 
TCCATTTCTG TTGGAGCCTT TCTGGATGAT GAGGATGAAA TGAGTTTGGA AGAGCTAAAA 
AACCGCCAAA ATGCAGCGCT AAAACACAGC TACTCTCACT CAGAGACAAT CACGGTGCCT 
GAGTGTATTA TGGAAACCAA CACAGTTCCT AGTGGTGAGC ACCAAAGTGG TACCAAACCT 
GGATTCTGTA GCCCATTATG CCATGATCAG TATCTTCCTG GGGAAAGTAG GCATAAAGGT 
GTCCATGAAG GAATGCTATC ACCAGTCTCT AACCTTATGG CTGAATTTGA TAAACTGGCA 
ATTTCAGAAA AAAGTGAGCT TGAGAATGAG ACATGGAACA TAGAGCCTTC TCTAATAGCT 
AAAGAAACTG AGCACAATTT ACATGCTGGT TCATCAACCA GGACAAATGG TTCAGTGTTA 
TCACAAGTTG AGCAAATGAT GCCAGAGCTC AGCCTGCAGG ATAAACTTTC TCCACCAAGA 
AACAGTATTG GTCTCTCACC CCCACGTGCC TCTTTGGACT CTTCCATTCA TCTGGGTATG 
AAGACTCCAC CAAGAAACCA ATGCAACAAA AGCATCTTCC TGCTGGGAAG GGAACCTTCC 
AAACTAGACA GTGATGTTCT GTCAGCAGTG GAAAATGTAG AACTCGATGG ACATCAGTAC 
CCCAGTGTTT CTAAATGGAT GCAAGCAGTG TTGTCTTATC CCAATACAGC TCGCCAAAGT 
TGGCCAAGTC CGGCACTTCT GTCAGGTAGA GGTAAGTCTC AGATGTTTTG TTCCAATTCA 
CCTGGAAGCC ATGCCTATTC TACGCCTGGG CGAAACAGTC CCATTCCTGG AAGCCCTGGG 
AAATACATGA ACACATCTGA TTATGGCAGC CCAGGAAGGT ATAGCCCTGC ATACGCCAGT 
CATATCCAAT TGCTACGTGT CAGACACTTT TCAGACCACC CTGCACTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910629.2
CDS	382..3372
Translation	MSGTHFSWDSVGGCQVLLACVLVLLGTWLFLLAERRRRHKSNPEPCLYPPEPCLYPPEPCLYPPEPCLYPPEPCLYPPEPCLYPPEPCLYPPEPCLSAPRPATEVQLVAQSMDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_004910572.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910572.3

ORF Insert Sequence:

ATGTCTGGCA CCCACTTCTC CTGGGATAGT GTGGGGGGCT GCCAGGTCCT GCTGGCCTGT 
GTACTGGTGC TGCTTGGCAC TTGGCTTTTC CTCCTGGCAG AAAGGAGGCG TAGGCACAAG 
TCTAACCCGG AGCCCTGTCT GTACCCCCCG GAGCCCTGTC TGTACCCCCC GGAGCCCTGT 
CTGTACCCCC CGGAGCCCTG TCTGTACCCC CCTGAGCCCT GTCTGTACCC CCCGGAGCCC 
TGTCTGTACC CCCCGGAGCC CTGTCTGTAC CCCCCTGAGC CCTGTCTGTC CGCCCCTCGG 
CCTGCTACCG AAGTGCAATT AGTGGCACAG AGTATGGATA ATATCCTTAG CCAGCTGAAG 
CTCCTCAACC CAGACCAGCT TAGAGAAGAA ATATTAAAGG CTGGATTAAA GTGTGGACCA 
ATCACATCCA CCACAAGATT TATATTTGAG AAGAAACTAG CACGTGTGTT ACTGGAGCAA 
CAGGGAGTGA ACTTAGAGGA AACCACAACT GTTTGTGATG GGAATGCAGG TGCGTCTGGG 
AATGCAGGCA CTGATAACAG GCAGCACACA AATTACACCC AGAAGAAAAA CTGCTTGGAA 
GATGGGGACT TTGGGTACAG TGTTGGATTA AATCCACCTT CCGAAGAAGC TCCGTTGGCA 
GGGAGAGGTT TTTCTGAAGT TAATGGAAGA TCGTTAGATG ATTGTCAGAC CTTCACCCCA 
ACATGTAAGG ATCCATCAAT GTTCTATGCT GTTTGCCCTG TCTATGAGGA GACACTAAAT 
AAAAATGAAA AAGTACATAT TTATGCAGAC AAGAAAGAAG CACTTCAAGT GGTTAAAATG 
TTAAAAGGTT CAAGGTTTAA GGCTTTTGTC TCCAGGGAGG ATGCTGAGAG GTTTGCAAGA 
GGCATATGTG ACTACTGCCC ATCACCTAGC AAGTCTTCAG TGATCCTGTC ACCTGTAAAA 
CTTTCACCAG CTCTAGTGAG AGATGGAATC AACTCTTCAG AGATGGAAAA CATCAACAAA 
GAAAGGGCAA ACAACTTCAA AAGTCCAAGG ACACAAGATC TAACTGCAAA GCTGAGGAAG 
GCTGTAGAGA AAGGAGATCA AACAACATTT TCAGATCTTA TTTGGACCAA TCCACGTTAT 
CTCATAGGTT CTGGAGATAA CCCTACTGTA GTGCAGGAAG GGTGCAGGTA TAATGTCATG 
CACGTGGCTG CCAAGGAGAA TCAGGCTGGT ATCTGCCAAC TCCTCTTAGA TACATTGGAA 
AATCCAGAAT TCATGCGGCT CATGTACCCA GATGATGATG ACCTCATGCT ACATAAGCGG 
ATCCGATATA TTGTTGATCT GTATCTAAAC ACTCCAGATA AAATGGTTTT TGACACTCCT 
TTGCACTTTG CTTGCAAGTT TGGGAATGCT GATGTTGTCA GTGTGCTATG CTCTCATCCT 
GACATTTTAA AAAATCCTCG AAACAAGTAT GAACAGACCC CGGAAGAGGC TGTCTGTGAA 
AGAAGCAAAA ATAAATCTGC AGAACTTAAG AATAAGATAA AAGAGTATTT AAAAGGTCAT 
TTTTATGTGC CACTGTTACG GGCTGAGGAT AACTCCTCGT TTCCAGTTAT AGGAGCTCCA 
TGGTCTCCCG AGCAGCCTGA CCTAATCCAG ACCAGATTCT CTGGCAGTCC CAAAGATCCG 
CTATTAGCTG TGAGAGCATT TGCAGGCCCT ATGAGCCCTT CTAAGGCAGA AGATTTCAGA 
AGAGTCTGGA AAACACCACC ACGGGAGAAA GCTGAATTTT ATCACAAAAT CAGAAAAACA 
GACCCAGAAA GAGGAGCAGA GAGAGTGGGA AGAGTGCTCG CTCATGAACT GGATGTGCCT 
TGGGTGGAGT ACTGGGATTT CCTTGGCTGC TTTGTTGACC TCTCTTCCCA GGAGGGTCTG 
AGGAAGCTGG AGGAATATCT GAGCAAGAGA GAAACAAGTG AAAGGGCACA GCGGGATTCA 
GATTATGAAA TATGTAACAA GTATAAAACG CCTTCAGGAA AGAGCAAAAA AATGTGCAAT 
TCCATTTCTG TTGGAGCCTT TCTGGATGAT GAGGATGAAA TGAGTTTGGA AGAGCTAAAA 
AACCGCCAAA ATGCAGCGCT AAAACACAGC TACTCTCACT CAGAGACAAT CACGGTGCCT 
GAGTGTATTA TGGAAACCAA CACAGTTCCT AGTGGTGAGC ACCAAAGTGG TACCAAACCT 
GGATTCTGTA GCCCATTATG CCATGATCAG TATCTTCCTG GGGAAAGTAG GCATAAAGGT 
GTCCATGAAG GAATGCTATC ACCAGTCTCT AACCTTATGG CTGAATTTGA TAAACTGGCA 
ATTTCAGAAA AAAGTGAGCT TGAGAATGAG ACATGGAACA TAGAGCCTTC TCTAATAGCT 
AAAGAAACTG AGCACAATTT ACATGCTGGT TCATCAACCA GGACAAATGG TTCAGTGTTA 
TCACAAGTTG AGCAAATGAT GCCAGAGCTC AGCCTGCAGG ATAAACTTTC TCCACCAAGA 
AACAGTATTG GTCTCTCACC CCCACGTGCC TCTTTGGACT CTTCCATTCA TCTGGGTATG 
AAGACTCCAC CAAGAAACCA ATGCAACAAA AGCATCTTCC TGCTGGGAAG GGAACCTTCC 
AAACTAGACA GTGATGTTCT GTCAGCAGTG GAAAATGTAG AACTCGATGG ACATCAGTAC 
CCCAGTGTTT CTAAATGGAT GCAAGCAGTG TTGTCTTATC CCAATACAGC TCGCCAAAGT 
TGGCCAAGTC CGGCACTTCT GTCAGGTAGA GGTAAGTCTC AGATGTTTTG TTCCAATTCA 
CCTGGAAGCC ATGCCTATTC TACGCCTGGG CGAAACAGTC CCATTCCTGG AAGCCCTGGG 
AAATACATGA ACACATCTGA TTATGGCAGC CCAGGAAGGT ATAGCCCTGC ATACGCCAGT 
CATATCCAAT TGCTACGTGT CAGACACTTT TCAGACCACC CTGCACTTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_012969394.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824848.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa48942
Clone ID Related Accession (Same CDS sequence)	XM_012969394.3
Accession Version	XM_012969394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GTATGGATAA TATCCTTAGC CAGCTGAAGC TCCTCAACCC AGACCAGCTT 
AGAGAAGAAA TATTAAAGGC TGGATTAAAG TGTGGACCAA TCACATCCAC CACAAGATTT 
ATATTTGAGA AGAAACTAGC ACGTGTGTTA CTGGAGCAAC AGGGAGTGAA CTTAGAGGAA 
ACCACAACTG TTTGTGATGG GAATGCAGGT GCGTCTGGGA ATGCAGGCAC TGATAACAGG 
CAGCACACAA ATTACACCCA GAAGAAAAAC TGCTTGGAAG ATGGGGACTT TGGGTACAGT 
GTTGGATTAA ATCCACCTTC CGAAGAAGCT CCGTTGGCAG GGAGAGGTTT TTCTGAAGTT 
AATGGAAGAT CGTTAGATGA TTGTCAGACC TTCACCCCAA CATGTAAGGA TCCATCAATG 
TTCTATGCTG TTTGCCCTGT CTATGAGGAG ACACTAAATA AAAATGAAAA AGTACATATT 
TATGCAGACA AGAAAGAAGC ACTTCAAGTG GTTAAAATGT TAAAAGGTTC AAGGTTTAAG 
GCTTTTGTCT CCAGGGAGGA TGCTGAGAGG TTTGCAAGAG GCATATGTGA CTACTGCCCA 
TCACCTAGCA AGTCTTCAGT GATCCTGTCA CCTGTAAAAC TTTCACCAGC TCTAGTGAGA 
GATGGAATCA ACTCTTCAGA GATGGAAAAC ATCAACAAAG AAAGGGCAAA CAACTTCAAA 
AGTCCAAGGA CACAAGATCT AACTGCAAAG CTGAGGAAGG CTGTAGAGAA AGGAGATCAA 
ACAACATTTT CAGATCTTAT TTGGACCAAT CCACGTTATC TCATAGGTTC TGGAGATAAC 
CCTACTGTAG TGCAGGAAGG GTGCAGGTAT AATGTCATGC ACGTGGCTGC CAAGGAGAAT 
CAGGCTGGTA TCTGCCAACT CCTCTTAGAT ACATTGGAAA ATCCAGAATT CATGCGGCTC 
ATGTACCCAG ATGATGATGA CCTCATGCTA CATAAGCGGA TCCGATATAT TGTTGATCTG 
TATCTAAACA CTCCAGATAA AATGGTTTTT GACACTCCTT TGCACTTTGC TTGCAAGTTT 
GGGAATGCTG ATGTTGTCAG TGTGCTATGC TCTCATCCTG ACATTTTAAA AAATCCTCGA 
AACAAGTATG AACAGACCCC GGAAGAGGCT GTCTGTGAAA GAAGCAAAAA TAAATCTGCA 
GAACTTAAGA ATAAGATAAA AGAGTATTTA AAAGGTCATT TTTATGTGCC ACTGTTACGG 
GCTGAGGATA ACTCCTCGTT TCCAGTTATA GGAGCTCCAT GGTCTCCCGA GCAGCCTGAC 
CTAATCCAGA CCAGATTCTC TGGCAGTCCC AAAGATCCGC TATTAGCTGT GAGAGCATTT 
GCAGGCCCTA TGAGCCCTTC TAAGGCAGAA GATTTCAGAA GAGTCTGGAA AACACCACCA 
CGGGAGAAAG CTGAATTTTA TCACAAAATC AGAAAAACAG ACCCAGAAAG AGGAGCAGAG 
AGAGTGGGAA GAGTGCTCGC TCATGAACTG GATGTGCCTT GGGTGGAGTA CTGGGATTTC 
CTTGGCTGCT TTGTTGACCT CTCTTCCCAG GAGGGTCTGA GGAAGCTGGA GGAATATCTG 
AGCAAGAGAG AAACAAGTGA AAGGGCACAG CGGGATTCAG ATTATGAAAT ATGTAACAAG 
TATAAAACGC CTTCAGGAAA GAGCAAAAAA ATGTGCAATT CCATTTCTGT TGGAGCCTTT 
CTGGATGATG AGGATGAAAT GAGTTTGGAA GAGCTAAAAA ACCGCCAAAA TGCAGCGCTA 
AAACACAGCT ACTCTCACTC AGAGACAATC ACGGTGCCTG AGTGTATTAT GGAAACCAAC 
ACAGTTCCTA GTGGTGAGCA CCAAAGTGGT ACCAAACCTG GATTCTGTAG CCCATTATGC 
CATGATCAGT ATCTTCCTGG GGAAAGTAGG CATAAAGGTG TCCATGAAGG AATGCTATCA 
CCAGTCTCTA ACCTTATGGC TGAATTTGAT AAACTGGCAA TTTCAGAAAA AAGTGAGCTT 
GAGAATGAGA CATGGAACAT AGAGCCTTCT CTAATAGCTA AAGAAACTGA GCACAATTTA 
CATGCTGGTT CATCAACCAG GACAAATGGT TCAGTGTTAT CACAAGTTGA GCAAATGATG 
CCAGAGCTCA GCCTGCAGGA TAAACTTTCT CCACCAAGAA ACAGTATTGG TCTCTCACCC 
CCACGTGCCT CTTTGGACTC TTCCATTCAT CTGGGTATGA AGACTCCACC AAGAAACCAA 
TGCAACAAAA GCATCTTCCT GCTGGGAAGG GAACCTTCCA AACTAGACAG TGATGTTCTG 
TCAGCAGTGG AAAATGTAGA ACTCGATGGA CATCAGTACC CCAGTGTTTC TAAATGGATG 
CAAGCAGTGT TGTCTTATCC CAATACAGCT CGCCAAAGTT GGCCAAGTCC GGCACTTCTG 
TCAGGTAGAG GTAAGTCTCA GATGTTTTGT TCCAATTCAC CTGGAAGCCA TGCCTATTCT 
ACGCCTGGGC GAAACAGTCC CATTCCTGGA AGCCCTGGGA AATACATGAA CACATCTGAT 
TATGGCAGCC CAGGAAGGTA TAGCCCTGCA TACGCCAGTC ATATCCAATT GCTACGTGTC 
AGACACTTTT CAGACCACCC TGCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824848.1
CDS	74..2743
Translation	MAQSMDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_012969394.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969394.3

ORF Insert Sequence:

ATGGCACAGA GTATGGATAA TATCCTTAGC CAGCTGAAGC TCCTCAACCC AGACCAGCTT 
AGAGAAGAAA TATTAAAGGC TGGATTAAAG TGTGGACCAA TCACATCCAC CACAAGATTT 
ATATTTGAGA AGAAACTAGC ACGTGTGTTA CTGGAGCAAC AGGGAGTGAA CTTAGAGGAA 
ACCACAACTG TTTGTGATGG GAATGCAGGT GCGTCTGGGA ATGCAGGCAC TGATAACAGG 
CAGCACACAA ATTACACCCA GAAGAAAAAC TGCTTGGAAG ATGGGGACTT TGGGTACAGT 
GTTGGATTAA ATCCACCTTC CGAAGAAGCT CCGTTGGCAG GGAGAGGTTT TTCTGAAGTT 
AATGGAAGAT CGTTAGATGA TTGTCAGACC TTCACCCCAA CATGTAAGGA TCCATCAATG 
TTCTATGCTG TTTGCCCTGT CTATGAGGAG ACACTAAATA AAAATGAAAA AGTACATATT 
TATGCAGACA AGAAAGAAGC ACTTCAAGTG GTTAAAATGT TAAAAGGTTC AAGGTTTAAG 
GCTTTTGTCT CCAGGGAGGA TGCTGAGAGG TTTGCAAGAG GCATATGTGA CTACTGCCCA 
TCACCTAGCA AGTCTTCAGT GATCCTGTCA CCTGTAAAAC TTTCACCAGC TCTAGTGAGA 
GATGGAATCA ACTCTTCAGA GATGGAAAAC ATCAACAAAG AAAGGGCAAA CAACTTCAAA 
AGTCCAAGGA CACAAGATCT AACTGCAAAG CTGAGGAAGG CTGTAGAGAA AGGAGATCAA 
ACAACATTTT CAGATCTTAT TTGGACCAAT CCACGTTATC TCATAGGTTC TGGAGATAAC 
CCTACTGTAG TGCAGGAAGG GTGCAGGTAT AATGTCATGC ACGTGGCTGC CAAGGAGAAT 
CAGGCTGGTA TCTGCCAACT CCTCTTAGAT ACATTGGAAA ATCCAGAATT CATGCGGCTC 
ATGTACCCAG ATGATGATGA CCTCATGCTA CATAAGCGGA TCCGATATAT TGTTGATCTG 
TATCTAAACA CTCCAGATAA AATGGTTTTT GACACTCCTT TGCACTTTGC TTGCAAGTTT 
GGGAATGCTG ATGTTGTCAG TGTGCTATGC TCTCATCCTG ACATTTTAAA AAATCCTCGA 
AACAAGTATG AACAGACCCC GGAAGAGGCT GTCTGTGAAA GAAGCAAAAA TAAATCTGCA 
GAACTTAAGA ATAAGATAAA AGAGTATTTA AAAGGTCATT TTTATGTGCC ACTGTTACGG 
GCTGAGGATA ACTCCTCGTT TCCAGTTATA GGAGCTCCAT GGTCTCCCGA GCAGCCTGAC 
CTAATCCAGA CCAGATTCTC TGGCAGTCCC AAAGATCCGC TATTAGCTGT GAGAGCATTT 
GCAGGCCCTA TGAGCCCTTC TAAGGCAGAA GATTTCAGAA GAGTCTGGAA AACACCACCA 
CGGGAGAAAG CTGAATTTTA TCACAAAATC AGAAAAACAG ACCCAGAAAG AGGAGCAGAG 
AGAGTGGGAA GAGTGCTCGC TCATGAACTG GATGTGCCTT GGGTGGAGTA CTGGGATTTC 
CTTGGCTGCT TTGTTGACCT CTCTTCCCAG GAGGGTCTGA GGAAGCTGGA GGAATATCTG 
AGCAAGAGAG AAACAAGTGA AAGGGCACAG CGGGATTCAG ATTATGAAAT ATGTAACAAG 
TATAAAACGC CTTCAGGAAA GAGCAAAAAA ATGTGCAATT CCATTTCTGT TGGAGCCTTT 
CTGGATGATG AGGATGAAAT GAGTTTGGAA GAGCTAAAAA ACCGCCAAAA TGCAGCGCTA 
AAACACAGCT ACTCTCACTC AGAGACAATC ACGGTGCCTG AGTGTATTAT GGAAACCAAC 
ACAGTTCCTA GTGGTGAGCA CCAAAGTGGT ACCAAACCTG GATTCTGTAG CCCATTATGC 
CATGATCAGT ATCTTCCTGG GGAAAGTAGG CATAAAGGTG TCCATGAAGG AATGCTATCA 
CCAGTCTCTA ACCTTATGGC TGAATTTGAT AAACTGGCAA TTTCAGAAAA AAGTGAGCTT 
GAGAATGAGA CATGGAACAT AGAGCCTTCT CTAATAGCTA AAGAAACTGA GCACAATTTA 
CATGCTGGTT CATCAACCAG GACAAATGGT TCAGTGTTAT CACAAGTTGA GCAAATGATG 
CCAGAGCTCA GCCTGCAGGA TAAACTTTCT CCACCAAGAA ACAGTATTGG TCTCTCACCC 
CCACGTGCCT CTTTGGACTC TTCCATTCAT CTGGGTATGA AGACTCCACC AAGAAACCAA 
TGCAACAAAA GCATCTTCCT GCTGGGAAGG GAACCTTCCA AACTAGACAG TGATGTTCTG 
TCAGCAGTGG AAAATGTAGA ACTCGATGGA CATCAGTACC CCAGTGTTTC TAAATGGATG 
CAAGCAGTGT TGTCTTATCC CAATACAGCT CGCCAAAGTT GGCCAAGTCC GGCACTTCTG 
TCAGGTAGAG GTAAGTCTCA GATGTTTTGT TCCAATTCAC CTGGAAGCCA TGCCTATTCT 
ACGCCTGGGC GAAACAGTCC CATTCCTGGA AGCCCTGGGA AATACATGAA CACATCTGAT 
TATGGCAGCC CAGGAAGGTA TAGCCCTGCA TACGCCAGTC ATATCCAATT GCTACGTGTC 
AGACACTTTT CAGACCACCC TGCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_002931879.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931925.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.

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CloneID	OXa48943
Clone ID Related Accession (Same CDS sequence)	XM_002931879.5
Accession Version	XM_002931879.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931879.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAATA TCCTTAGCCA GCTGAAGCTC CTCAACCCAG ACCAGCTTAG AGAAGAAATA 
TTAAAGGCTG GATTAAAGTG TGGACCAATC ACATCCACCA CAAGATTTAT ATTTGAGAAG 
AAACTAGCAC GTGTGTTACT GGAGCAACAG GGAGTGAACT TAGAGGAAAC CACAACTGTT 
TGTGATGGGA ATGCAGGTGC GTCTGGGAAT GCAGGCACTG ATAACAGGCA GCACACAAAT 
TACACCCAGA AGAAAAACTG CTTGGAAGAT GGGGACTTTG GGTACAGTGT TGGATTAAAT 
CCACCTTCCG AAGAAGCTCC GTTGGCAGGG AGAGGTTTTT CTGAAGTTAA TGGAAGATCG 
TTAGATGATT GTCAGACCTT CACCCCAACA TGTAAGGATC CATCAATGTT CTATGCTGTT 
TGCCCTGTCT ATGAGGAGAC ACTAAATAAA AATGAAAAAG TACATATTTA TGCAGACAAG 
AAAGAAGCAC TTCAAGTGGT TAAAATGTTA AAAGGTTCAA GGTTTAAGGC TTTTGTCTCC 
AGGGAGGATG CTGAGAGGTT TGCAAGAGGC ATATGTGACT ACTGCCCATC ACCTAGCAAG 
TCTTCAGTGA TCCTGTCACC TGTAAAACTT TCACCAGCTC TAGTGAGAGA TGGAATCAAC 
TCTTCAGAGA TGGAAAACAT CAACAAAGAA AGGGCAAACA ACTTCAAAAG TCCAAGGACA 
CAAGATCTAA CTGCAAAGCT GAGGAAGGCT GTAGAGAAAG GAGATCAAAC AACATTTTCA 
GATCTTATTT GGACCAATCC ACGTTATCTC ATAGGTTCTG GAGATAACCC TACTGTAGTG 
CAGGAAGGGT GCAGGTATAA TGTCATGCAC GTGGCTGCCA AGGAGAATCA GGCTGGTATC 
TGCCAACTCC TCTTAGATAC ATTGGAAAAT CCAGAATTCA TGCGGCTCAT GTACCCAGAT 
GATGATGACC TCATGCTACA TAAGCGGATC CGATATATTG TTGATCTGTA TCTAAACACT 
CCAGATAAAA TGGTTTTTGA CACTCCTTTG CACTTTGCTT GCAAGTTTGG GAATGCTGAT 
GTTGTCAGTG TGCTATGCTC TCATCCTGAC ATTTTAAAAA ATCCTCGAAA CAAGTATGAA 
CAGACCCCGG AAGAGGCTGT CTGTGAAAGA AGCAAAAATA AATCTGCAGA ACTTAAGAAT 
AAGATAAAAG AGTATTTAAA AGGTCATTTT TATGTGCCAC TGTTACGGGC TGAGGATAAC 
TCCTCGTTTC CAGTTATAGG AGCTCCATGG TCTCCCGAGC AGCCTGACCT AATCCAGACC 
AGATTCTCTG GCAGTCCCAA AGATCCGCTA TTAGCTGTGA GAGCATTTGC AGGCCCTATG 
AGCCCTTCTA AGGCAGAAGA TTTCAGAAGA GTCTGGAAAA CACCACCACG GGAGAAAGCT 
GAATTTTATC ACAAAATCAG AAAAACAGAC CCAGAAAGAG GAGCAGAGAG AGTGGGAAGA 
GTGCTCGCTC ATGAACTGGA TGTGCCTTGG GTGGAGTACT GGGATTTCCT TGGCTGCTTT 
GTTGACCTCT CTTCCCAGGA GGGTCTGAGG AAGCTGGAGG AATATCTGAG CAAGAGAGAA 
ACAAGTGAAA GGGCACAGCG GGATTCAGAT TATGAAATAT GTAACAAGTA TAAAACGCCT 
TCAGGAAAGA GCAAAAAAAT GTGCAATTCC ATTTCTGTTG GAGCCTTTCT GGATGATGAG 
GATGAAATGA GTTTGGAAGA GCTAAAAAAC CGCCAAAATG CAGCGCTAAA ACACAGCTAC 
TCTCACTCAG AGACAATCAC GGTGCCTGAG TGTATTATGG AAACCAACAC AGTTCCTAGT 
GGTGAGCACC AAAGTGGTAC CAAACCTGGA TTCTGTAGCC CATTATGCCA TGATCAGTAT 
CTTCCTGGGG AAAGTAGGCA TAAAGGTGTC CATGAAGGAA TGCTATCACC AGTCTCTAAC 
CTTATGGCTG AATTTGATAA ACTGGCAATT TCAGAAAAAA GTGAGCTTGA GAATGAGACA 
TGGAACATAG AGCCTTCTCT AATAGCTAAA GAAACTGAGC ACAATTTACA TGCTGGTTCA 
TCAACCAGGA CAAATGGTTC AGTGTTATCA CAAGTTGAGC AAATGATGCC AGAGCTCAGC 
CTGCAGGATA AACTTTCTCC ACCAAGAAAC AGTATTGGTC TCTCACCCCC ACGTGCCTCT 
TTGGACTCTT CCATTCATCT GGGTATGAAG ACTCCACCAA GAAACCAATG CAACAAAAGC 
ATCTTCCTGC TGGGAAGGGA ACCTTCCAAA CTAGACAGTG ATGTTCTGTC AGCAGTGGAA 
AATGTAGAAC TCGATGGACA TCAGTACCCC AGTGTTTCTA AATGGATGCA AGCAGTGTTG 
TCTTATCCCA ATACAGCTCG CCAAAGTTGG CCAAGTCCGG CACTTCTGTC AGGTAGAGGT 
AAGTCTCAGA TGTTTTGTTC CAATTCACCT GGAAGCCATG CCTATTCTAC GCCTGGGCGA 
AACAGTCCCA TTCCTGGAAG CCCTGGGAAA TACATGAACA CATCTGATTA TGGCAGCCCA 
GGAAGGTATA GCCCTGCATA CGCCAGTCAT ATCCAATTGC TACGTGTCAG ACACTTTTCA 
GACCACCCTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931925.1
CDS	350..3007
Translation	MDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_002931879.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931879.5

ORF Insert Sequence:

ATGGATAATA TCCTTAGCCA GCTGAAGCTC CTCAACCCAG ACCAGCTTAG AGAAGAAATA 
TTAAAGGCTG GATTAAAGTG TGGACCAATC ACATCCACCA CAAGATTTAT ATTTGAGAAG 
AAACTAGCAC GTGTGTTACT GGAGCAACAG GGAGTGAACT TAGAGGAAAC CACAACTGTT 
TGTGATGGGA ATGCAGGTGC GTCTGGGAAT GCAGGCACTG ATAACAGGCA GCACACAAAT 
TACACCCAGA AGAAAAACTG CTTGGAAGAT GGGGACTTTG GGTACAGTGT TGGATTAAAT 
CCACCTTCCG AAGAAGCTCC GTTGGCAGGG AGAGGTTTTT CTGAAGTTAA TGGAAGATCG 
TTAGATGATT GTCAGACCTT CACCCCAACA TGTAAGGATC CATCAATGTT CTATGCTGTT 
TGCCCTGTCT ATGAGGAGAC ACTAAATAAA AATGAAAAAG TACATATTTA TGCAGACAAG 
AAAGAAGCAC TTCAAGTGGT TAAAATGTTA AAAGGTTCAA GGTTTAAGGC TTTTGTCTCC 
AGGGAGGATG CTGAGAGGTT TGCAAGAGGC ATATGTGACT ACTGCCCATC ACCTAGCAAG 
TCTTCAGTGA TCCTGTCACC TGTAAAACTT TCACCAGCTC TAGTGAGAGA TGGAATCAAC 
TCTTCAGAGA TGGAAAACAT CAACAAAGAA AGGGCAAACA ACTTCAAAAG TCCAAGGACA 
CAAGATCTAA CTGCAAAGCT GAGGAAGGCT GTAGAGAAAG GAGATCAAAC AACATTTTCA 
GATCTTATTT GGACCAATCC ACGTTATCTC ATAGGTTCTG GAGATAACCC TACTGTAGTG 
CAGGAAGGGT GCAGGTATAA TGTCATGCAC GTGGCTGCCA AGGAGAATCA GGCTGGTATC 
TGCCAACTCC TCTTAGATAC ATTGGAAAAT CCAGAATTCA TGCGGCTCAT GTACCCAGAT 
GATGATGACC TCATGCTACA TAAGCGGATC CGATATATTG TTGATCTGTA TCTAAACACT 
CCAGATAAAA TGGTTTTTGA CACTCCTTTG CACTTTGCTT GCAAGTTTGG GAATGCTGAT 
GTTGTCAGTG TGCTATGCTC TCATCCTGAC ATTTTAAAAA ATCCTCGAAA CAAGTATGAA 
CAGACCCCGG AAGAGGCTGT CTGTGAAAGA AGCAAAAATA AATCTGCAGA ACTTAAGAAT 
AAGATAAAAG AGTATTTAAA AGGTCATTTT TATGTGCCAC TGTTACGGGC TGAGGATAAC 
TCCTCGTTTC CAGTTATAGG AGCTCCATGG TCTCCCGAGC AGCCTGACCT AATCCAGACC 
AGATTCTCTG GCAGTCCCAA AGATCCGCTA TTAGCTGTGA GAGCATTTGC AGGCCCTATG 
AGCCCTTCTA AGGCAGAAGA TTTCAGAAGA GTCTGGAAAA CACCACCACG GGAGAAAGCT 
GAATTTTATC ACAAAATCAG AAAAACAGAC CCAGAAAGAG GAGCAGAGAG AGTGGGAAGA 
GTGCTCGCTC ATGAACTGGA TGTGCCTTGG GTGGAGTACT GGGATTTCCT TGGCTGCTTT 
GTTGACCTCT CTTCCCAGGA GGGTCTGAGG AAGCTGGAGG AATATCTGAG CAAGAGAGAA 
ACAAGTGAAA GGGCACAGCG GGATTCAGAT TATGAAATAT GTAACAAGTA TAAAACGCCT 
TCAGGAAAGA GCAAAAAAAT GTGCAATTCC ATTTCTGTTG GAGCCTTTCT GGATGATGAG 
GATGAAATGA GTTTGGAAGA GCTAAAAAAC CGCCAAAATG CAGCGCTAAA ACACAGCTAC 
TCTCACTCAG AGACAATCAC GGTGCCTGAG TGTATTATGG AAACCAACAC AGTTCCTAGT 
GGTGAGCACC AAAGTGGTAC CAAACCTGGA TTCTGTAGCC CATTATGCCA TGATCAGTAT 
CTTCCTGGGG AAAGTAGGCA TAAAGGTGTC CATGAAGGAA TGCTATCACC AGTCTCTAAC 
CTTATGGCTG AATTTGATAA ACTGGCAATT TCAGAAAAAA GTGAGCTTGA GAATGAGACA 
TGGAACATAG AGCCTTCTCT AATAGCTAAA GAAACTGAGC ACAATTTACA TGCTGGTTCA 
TCAACCAGGA CAAATGGTTC AGTGTTATCA CAAGTTGAGC AAATGATGCC AGAGCTCAGC 
CTGCAGGATA AACTTTCTCC ACCAAGAAAC AGTATTGGTC TCTCACCCCC ACGTGCCTCT 
TTGGACTCTT CCATTCATCT GGGTATGAAG ACTCCACCAA GAAACCAATG CAACAAAAGC 
ATCTTCCTGC TGGGAAGGGA ACCTTCCAAA CTAGACAGTG ATGTTCTGTC AGCAGTGGAA 
AATGTAGAAC TCGATGGACA TCAGTACCCC AGTGTTTCTA AATGGATGCA AGCAGTGTTG 
TCTTATCCCA ATACAGCTCG CCAAAGTTGG CCAAGTCCGG CACTTCTGTC AGGTAGAGGT 
AAGTCTCAGA TGTTTTGTTC CAATTCACCT GGAAGCCATG CCTATTCTAC GCCTGGGCGA 
AACAGTCCCA TTCCTGGAAG CCCTGGGAAA TACATGAACA CATCTGATTA TGGCAGCCCA 
GGAAGGTATA GCCCTGCATA CGCCAGTCAT ATCCAATTGC TACGTGTCAG ACACTTTTCA 
GACCACCCTG CACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ankle2 ( XM_004910572.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910629.1 Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03602
Clone ID Related Accession (Same CDS sequence)	XM_004910572.2
Accession Version	XM_004910572.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004910572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCA CCCACTTCTC CTGGGATAGT GTGGGGGGCT GCCAGGTCCT GCTGGCCTGT 
GTACTGGTGC TGCTTGGCAC TTGGCTTTTC CTCCTGGCAG AAAGGAGGCG TAGGCACAAG 
TCTAACCCTG AGCCCTGTCT GTACCCCCCT GAGCCCTGTC TGTACCCCCC TGAGCCCTGT 
CTGTACCTCC CTGAGCCCTG TCTGTACCCC CCTGAGCCCT GTCTGTCCGC CCCTGAGCCC 
TGTCTGTCCG CCCCTCGGCC TGCTACCGAA GTTCAATTAG TGGCACAGAG TATGGATAAT 
ATCCTTAGCC AGCTGAAGCT CCTCAACCCA GACCAGCTTA GAGAAGAAAT ATTAAAGGCT 
GGATTAAAGT GTGGACCAAT CACATCCACC ACAAGATTTA TATTTGAGAA GAAACTAGCA 
CGTGTGTTAC TGGAGCAACA GGGAGTGAAC TTAGAGGAAA CCACAACTGT TTGTGATGGG 
AATGCAGGTG CGTCTGGGAA TGCAGGCACT GATAACAGGC AGCACACAAA TTACACCCAG 
AAGAAAAACT GCTTGGAAGA TGGGGACTTT GGGTACAGTG TTGGATTAAA TCCACCTTCC 
GAAGAAGCTC CGTTGGCAGG GAGAGGTTTT TCTGAAGTTA ATGGAAGATC GTTAGATGAT 
TGTCAGACCT TCACCCCAAC ATGTAAGGAT CCATCAATGT TCTATGCTGT TTGCCCTGTC 
TATGAGGAGA CACTAAATAA AAATGAAAAA GTACATATTT ATGCAGACAA GAAAGAAGCA 
CTTCAAGTGG TTAAAATGTT AAAAGGTTCA AGGTTTAAGG CTTTTGTCTC CAGGGAGGAT 
GCTGAGAGGT TTGCAAGAGG CATATGTGAC TACTGCCCAT CACCTAGCAA GTCTTCAGTG 
ATCCTGTCAC CTGTAAAACT TTCACCAGCT CTAGTGAGAG ATGGAATCAA CTCTTCAGAG 
ATGGAAAACA TCAACAAAGA AAGGGCAAAC AACTTCAAAA GTCCAAGGAC ACAAGATCTA 
ACTGCAAAGC TGAGGAAGGC TGTAGAGAAA GGAGATCAAA CAACATTTTC AGATCTTATT 
TGGACCAATC CACGTTATCT CATAGGTTCT GGAGATAACC CTACTGTAGT GCAGGAAGGG 
TGCAGGTATA ATGTCATGCA CGTGGCTGCC AAGGAGAATC AGGCTGGTAT CTGCCAACTC 
CTCTTAGATA CATTGGAAAA TCCAGAATTC ATGCGGCTCA TGTACCCAGA TGATGATGAC 
CTCATGCTAC ATAAGCGGAT CCGATATATT GTTGATCTGT ATCTAAACAC TCCAGATAAA 
ATGGTTTTTG ACACTCCTTT GCACTTTGCT TGCAAGTTTG GGAATGCTGA TGTTGTCAGT 
GTGCTATGCT CTCATCCTGA CATTTTAAAA AATCCTCGAA ACAAGTATGA ACAGACCCCG 
GAAGAGGCTG TCTGTGAAAG AAGCAAAAAT AAATCTGCAG AACTTAAGAA TAAGATAAAA 
GAGTATTTAA AAGGTCATTT TTATGTGCCA CTGTTACGGG CTGAGGATAA CTCCTCGTTT 
CCAGTTATAG GAGCTCCATG GTCTCCCGAG CAGCCTGACC TAATCCAGAC CAGATTCTCT 
GGCAGTCCCA AAGATCCGCT ATTAGCTGTG AGAGCATTTG CAGGCCCTAT GAGCCCTTCT 
AAGGCAGAAG ATTTCAGAAG AGTCTGGAAA ACACCACCAC GGGAGAAAGC TGAATTTTAT 
CACAAAATCA GAAAAACAGA CCCAGAAAGA GGAGCAGAGA GAGTGGGAAG AGTGCTCGCT 
CATGAACTGG ATGTGCCTTG GGTGGAGTAC TGGGATTTCC TTGGCTGCTT TGTTGACCTC 
TCTTCCCAGG AGGGTCTGAG GAAGCTGGAG GAATATCTGA GCAAGAGAGA AACAAGTGAA 
AGGGCACAGC GGGATTCAGA TTATGAAATC TGTAACAAGT ATAAAACGCC TTCAGGAAAG 
AGCAAAAAAA TGTGCAATTC CATTTCTGTT GGAGCCTTTC TGGATGATGA GGATGAAATG 
AGTTTGGAAG AGCTAAAAAA CCGCCAAAAT GCAGCGCTAA AACACAGCTA CTCTCACTCA 
GAGACAATCA CGGTGCCTGA GTGTATTATG GAAACCAACA CAGTTCCTAG TGGTGAGCAC 
CAAAGTGGTA CCAAACCTGG ATTCTGTAGC CCATTATGCC ATGATCAGTA TCTTCCTGGG 
GAAAGTAGGC ATAAAGGTGT CCATGAAGGA ATGCTATCAC CAGTCTCTAA CCTTATGGCT 
GAATTTGATA AACTGGCAAT TTCAGAAAAA AGTGAGCTTG AGAATGAGAC ATGGAACATA 
GAGCCTTCTC TAATAGCTAA AGAAACTGAG CACAATTTAC ATGCTGGTTC ATCAACCAGG 
ACAAATGGTT CAGTGTTATC ACAAGTTGAG CAAATGATGC CAGAGCTCAG CCTGCAGGAT 
AAACTTTCTC CACCAAGAAA CAGTATTGGT CTCTCACCCC CACGTGCCTC TTTGGACTCT 
TCCATTCATC TGGGTATGAA GACTCCACCA AGAAACCAAT GCAACAAAAG CATCTTCCTG 
CTGGGAAGGG AACCTTCCAA ACTAGACAGT GATGTTCTGT CAGCAGTGGA AAATGTAGAA 
CTCGATGGAC ATCAGTACCC CAGTGTTTCT AAATGGATGC AAGCAGTGTT GTCTTATCCC 
AATACAGCTC GCCAAAGTTG GCCAAGTCCG GCACTTCTGT CAGGTAGAGG TAAGTCTCAG 
ATGTTTTGTT CCAATTCACC TGGAAGCCAT GCCTATTCTA CGCCTGGGCG AAACAGTCCC 
ATTCCTGGAA GCCCTGGGAA ATACATGAAC ACATCTGATT ATGGCAGCCC AGGAAGGTAT 
AGCCCTGCAT ACGCCAGTCA TATCCAATTG CTACGTGTCA GACACTTTTC AGACCACCCT 
GCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910629.1
CDS	382..3330
Translation	MSGTHFSWDSVGGCQVLLACVLVLLGTWLFLLAERRRRHKSNPEPCLYPPEPCLYPPEPCLYLPEPCLYPPEPCLSAPEPCLSAPRPATEVQLVAQSMDNILSQLKLLNPDQLREEILKAGLKCGPITSTTRFIFEKKLARVLLEQQGVNLEETTTVCDGNAGASGNAGTDNRQHTNYTQKKNCLEDGDFGYSVGLNPPSEEAPLAGRGFSEVNGRSLDDCQTFTPTCKDPSMFYAVCPVYEETLNKNEKVHIYADKKEALQVVKMLKGSRFKAFVSREDAERFARGICDYCPSPSKSSVILSPVKLSPALVRDGINSSEMENINKERANNFKSPRTQDLTAKLRKAVEKGDQTTFSDLIWTNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQAGICQLLLDTLENPEFMRLMYPDDDDLMLHKRIRYIVDLYLNTPDKMVFDTPLHFACKFGNADVVSVLCSHPDILKNPRNKYEQTPEEAVCERSKNKSAELKNKIKEYLKGHFYVPLLRAEDNSSFPVIGAPWSPEQPDLIQTRFSGSPKDPLLAVRAFAGPMSPSKAEDFRRVWKTPPREKAEFYHKIRKTDPERGAERVGRVLAHELDVPWVEYWDFLGCFVDLSSQEGLRKLEEYLSKRETSERAQRDSDYEICNKYKTPSGKSKKMCNSISVGAFLDDEDEMSLEELKNRQNAALKHSYSHSETITVPECIMETNTVPSGEHQSGTKPGFCSPLCHDQYLPGESRHKGVHEGMLSPVSNLMAEFDKLAISEKSELENETWNIEPSLIAKETEHNLHAGSSTRTNGSVLSQVEQMMPELSLQDKLSPPRNSIGLSPPRASLDSSIHLGMKTPPRNQCNKSIFLLGREPSKLDSDVLSAVENVELDGHQYPSVSKWMQAVLSYPNTARQSWPSPALLSGRGKSQMFCSNSPGSHAYSTPGRNSPIPGSPGKYMNTSDYGSPGRYSPAYASHIQLLRVRHFSDHPAL

Target ORF information:

RefSeq Version	XM_004910572.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ankyrin repeat and LEM domain containing 2 (ankle2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910572.2

ORF Insert Sequence:

ATGTCTGGCA CCCACTTCTC CTGGGATAGT GTGGGGGGCT GCCAGGTCCT GCTGGCCTGT 
GTACTGGTGC TGCTTGGCAC TTGGCTTTTC CTCCTGGCAG AAAGGAGGCG TAGGCACAAG 
TCTAACCCTG AGCCCTGTCT GTACCCCCCT GAGCCCTGTC TGTACCCCCC TGAGCCCTGT 
CTGTACCTCC CTGAGCCCTG TCTGTACCCC CCTGAGCCCT GTCTGTCCGC CCCTGAGCCC 
TGTCTGTCCG CCCCTCGGCC TGCTACCGAA GTTCAATTAG TGGCACAGAG TATGGATAAT 
ATCCTTAGCC AGCTGAAGCT CCTCAACCCA GACCAGCTTA GAGAAGAAAT ATTAAAGGCT 
GGATTAAAGT GTGGACCAAT CACATCCACC ACAAGATTTA TATTTGAGAA GAAACTAGCA 
CGTGTGTTAC TGGAGCAACA GGGAGTGAAC TTAGAGGAAA CCACAACTGT TTGTGATGGG 
AATGCAGGTG CGTCTGGGAA TGCAGGCACT GATAACAGGC AGCACACAAA TTACACCCAG 
AAGAAAAACT GCTTGGAAGA TGGGGACTTT GGGTACAGTG TTGGATTAAA TCCACCTTCC 
GAAGAAGCTC CGTTGGCAGG GAGAGGTTTT TCTGAAGTTA ATGGAAGATC GTTAGATGAT 
TGTCAGACCT TCACCCCAAC ATGTAAGGAT CCATCAATGT TCTATGCTGT TTGCCCTGTC 
TATGAGGAGA CACTAAATAA AAATGAAAAA GTACATATTT ATGCAGACAA GAAAGAAGCA 
CTTCAAGTGG TTAAAATGTT AAAAGGTTCA AGGTTTAAGG CTTTTGTCTC CAGGGAGGAT 
GCTGAGAGGT TTGCAAGAGG CATATGTGAC TACTGCCCAT CACCTAGCAA GTCTTCAGTG 
ATCCTGTCAC CTGTAAAACT TTCACCAGCT CTAGTGAGAG ATGGAATCAA CTCTTCAGAG 
ATGGAAAACA TCAACAAAGA AAGGGCAAAC AACTTCAAAA GTCCAAGGAC ACAAGATCTA 
ACTGCAAAGC TGAGGAAGGC TGTAGAGAAA GGAGATCAAA CAACATTTTC AGATCTTATT 
TGGACCAATC CACGTTATCT CATAGGTTCT GGAGATAACC CTACTGTAGT GCAGGAAGGG 
TGCAGGTATA ATGTCATGCA CGTGGCTGCC AAGGAGAATC AGGCTGGTAT CTGCCAACTC 
CTCTTAGATA CATTGGAAAA TCCAGAATTC ATGCGGCTCA TGTACCCAGA TGATGATGAC 
CTCATGCTAC ATAAGCGGAT CCGATATATT GTTGATCTGT ATCTAAACAC TCCAGATAAA 
ATGGTTTTTG ACACTCCTTT GCACTTTGCT TGCAAGTTTG GGAATGCTGA TGTTGTCAGT 
GTGCTATGCT CTCATCCTGA CATTTTAAAA AATCCTCGAA ACAAGTATGA ACAGACCCCG 
GAAGAGGCTG TCTGTGAAAG AAGCAAAAAT AAATCTGCAG AACTTAAGAA TAAGATAAAA 
GAGTATTTAA AAGGTCATTT TTATGTGCCA CTGTTACGGG CTGAGGATAA CTCCTCGTTT 
CCAGTTATAG GAGCTCCATG GTCTCCCGAG CAGCCTGACC TAATCCAGAC CAGATTCTCT 
GGCAGTCCCA AAGATCCGCT ATTAGCTGTG AGAGCATTTG CAGGCCCTAT GAGCCCTTCT 
AAGGCAGAAG ATTTCAGAAG AGTCTGGAAA ACACCACCAC GGGAGAAAGC TGAATTTTAT 
CACAAAATCA GAAAAACAGA CCCAGAAAGA GGAGCAGAGA GAGTGGGAAG AGTGCTCGCT 
CATGAACTGG ATGTGCCTTG GGTGGAGTAC TGGGATTTCC TTGGCTGCTT TGTTGACCTC 
TCTTCCCAGG AGGGTCTGAG GAAGCTGGAG GAATATCTGA GCAAGAGAGA AACAAGTGAA 
AGGGCACAGC GGGATTCAGA TTATGAAATC TGTAACAAGT ATAAAACGCC TTCAGGAAAG 
AGCAAAAAAA TGTGCAATTC CATTTCTGTT GGAGCCTTTC TGGATGATGA GGATGAAATG 
AGTTTGGAAG AGCTAAAAAA CCGCCAAAAT GCAGCGCTAA AACACAGCTA CTCTCACTCA 
GAGACAATCA CGGTGCCTGA GTGTATTATG GAAACCAACA CAGTTCCTAG TGGTGAGCAC 
CAAAGTGGTA CCAAACCTGG ATTCTGTAGC CCATTATGCC ATGATCAGTA TCTTCCTGGG 
GAAAGTAGGC ATAAAGGTGT CCATGAAGGA ATGCTATCAC CAGTCTCTAA CCTTATGGCT 
GAATTTGATA AACTGGCAAT TTCAGAAAAA AGTGAGCTTG AGAATGAGAC ATGGAACATA 
GAGCCTTCTC TAATAGCTAA AGAAACTGAG CACAATTTAC ATGCTGGTTC ATCAACCAGG 
ACAAATGGTT CAGTGTTATC ACAAGTTGAG CAAATGATGC CAGAGCTCAG CCTGCAGGAT 
AAACTTTCTC CACCAAGAAA CAGTATTGGT CTCTCACCCC CACGTGCCTC TTTGGACTCT 
TCCATTCATC TGGGTATGAA GACTCCACCA AGAAACCAAT GCAACAAAAG CATCTTCCTG 
CTGGGAAGGG AACCTTCCAA ACTAGACAGT GATGTTCTGT CAGCAGTGGA AAATGTAGAA 
CTCGATGGAC ATCAGTACCC CAGTGTTTCT AAATGGATGC AAGCAGTGTT GTCTTATCCC 
AATACAGCTC GCCAAAGTTG GCCAAGTCCG GCACTTCTGT CAGGTAGAGG TAAGTCTCAG 
ATGTTTTGTT CCAATTCACC TGGAAGCCAT GCCTATTCTA CGCCTGGGCG AAACAGTCCC 
ATTCCTGGAA GCCCTGGGAA ATACATGAAC ACATCTGATT ATGGCAGCCC AGGAAGGTAT 
AGCCCTGCAT ACGCCAGTCA TATCCAATTG CTACGTGTCA GACACTTTTC AGACCACCCT 
GCACTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ankle2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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