wfs1b cDNA ORF clone, Danio rerio(zebrafish)

The following wfs1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wfs1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa19454 XM_679418.7
Latest version!
Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa148810 XM_021481141.1
Latest version!
Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
ODa19454 XM_679418.6 Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa19454
    Clone ID Related Accession (Same CDS sequence) XM_679418.6 , XM_679418.7
    Accession Version XM_679418.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product wolframin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679418.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACACGT CTCTGCTTGA ATCTCAGCTG TCGGCCCCAG CATCACCTGT TTCTCCAGCA 
    TTCTCTGTGT CTGGACACAA CATTCATCCA GTGTCCTCTT CTTCAACACC CAGCCCCGCT
    CCTTCAGCCA TGACTCCGCC CCCACATCAC GCCTCTCAGA TGGGTCGATC CCAGCTGAAC
    GCAGCCAGCA ATTCCAGCAC TGCCTTTCCA GGACAATCAG CGTTTCCTCC TCAGGGGGAA
    ATCAAAGAGG AGCTGAGCAT TGATGATCTT AAACAGAGGG CAAAGAATGG AGATGCTAAA
    GCACAGACAG AGATTGGTAG ATATTACCTG AGACTAGCCG AGCAAGAAGA TGAAGAGGTG
    AATGCTGTTA CTGCTGTAAC ATGGCTACTC CAGGCAGCCA AAAATGGACG AAAAGATGCA
    GTGAAGTTGC TACAGCGCTG CCTGCATGAC AGGAGGGGCA TCACAGCGGA GAACAGAGAG
    GAGGTGTGTT CCCTGGCATG TGAATCTCGT TTTCAGCGCA GCGTAAGGAA AGCAGCTCTG
    CTCATGTATT GGAAACTCAA TCCAGAACGC AAGAAAAACA TCACAGCCTC TGAACTTCTG
    GAAAATGTGA GCCAGCTCAA CACAGATGGC GCTTCCCGCA GCCCTCTCTC CAGCCCAATA
    CAGAAACAAA GAAAGATTTT GGAGAGTTTG GTGTCAAGAG ATGAGACTGA TTGCATTGGT
    GTAGAAGAGT TTGTTGAGAA TACCAAACAA TACGCTGAGG GTATTTCACC ATCCATGGAA
    GGGGTAGCAG GGGATGATGA TGACGATGAT GAAGAGCCAG TAAAACATCC AGATGAGCTG
    CCTTTGCACC AGAAGGTGTT GAAGTTTCCT CTTCATGCAG TGTTGGAGGT GAAAGAGGTG
    TTGATTGACT GGGCGTCGCG TGCAGGCATG CAGTGGATTA GCGCCCTTAT ACCTACTCAT
    CATGTCAATA CCCTCATCTT CTTCTTCATC ATTTCCAACC TCACCCTGGA GTTCTTTGTC
    CTCGTCATCC CCCTAATTAT ATTCTACCTC TCCTTTGTGT CAATGGTCAT CTGCACCCTA
    CGAGTCTTCC AGAACAGCAA GGCATGGGAG AACTTCAGAG CGTTGACCGT CATGTTGTCT
    CATTTTGAGC CGGGGATTGA CCTGGAGCAG GCCGAGTCTA ACTTCACATG GAATCACCTT
    GAGCCATACC TCTACTTTCT CCTTTCTACG CTTTTCCTTA TCTTTTCGTT TCCTGTGGCT
    GACAAGTCCT GGTTGCCATG TTCAGAACTG GCTATGGTTG CAGTCTTCTT CACTGTAAGC
    AGCTACTTGA GCCTGCGACC ATCGGCACAA CAGCATGCCA AATTTGCTCT GCTTTCCCAG
    GTTGCCTCCG CCATTTTTAC ATTCATTAAT CGGTTGCTGG GAGGCTGGGT GGGTCGTATA
    ATTGGCGGCG CTTGGTTCAG CATGCGCCTT GGTGACTGGT TGGTAATGCA TGTAGGTGTG
    CCTTGTGTTC TCTATCTCTA CCTACTGTTC TTGTGTACCC GTATGGCCAC AGCAGGTGGT
    GCACGTGGCA CCTACTGTGT GCTTCTTCCC AACCTAGTGT GCTTCATCTG GTGTGAAGTG
    TGTGTTACGC TGCTGCAAGA GTCCACTGTA TTGGGGCTAA TGCGCTCAGT TGTGGGCTAC
    CTACTTTTTT TCTTTGCTCT GCCCGTGCTG TCGCTGGCTC TAGCAGCTGT AATGCTGGTT
    CAGCTGGTGC AGTGGTTCCT CGCCCTGGAA CTGACCAAAA TGATCGTAAC AGTGTGCATC
    TGCGTGCTGC CTGTCCTGCT TCGCTGGTGG ACTCGTTTTA GCGTGTCGCC TTTGGCAGTG
    CTTCGCTCTT TGCGTCGTAG CAGTGTGGTG AAGTTGATCC TTGTGTGGAT TTCAGCGCTG
    GTGCTCTTCA GCTGGTTCTA CGTGTACCGT TCGGAAGGGA TGAAAGTATA CAACTCCACC
    CTGACCTGGC AGGAGTACAG TGACCTGTGC GGCCCTCGTG CCTGGAAGGA GCGTAACATG
    GCACATGCCC AGATCATCTG CAGCCACTTA GAGGGACACC GAGTGACGTG GGAGGGACGG
    TTCAAGTATG TTCGTGTCAC TGAGATTGAG AATGGGGCAC AAGCTGTCGT CAATCTTCTA
    CCAGTCTTGA TAGCAGACTG GGTCCGATGT CTGTATGGAG AGGAGTACCC TGCTTGTGAT
    GAGAATCAGA CGGGACCTGC TGAACCTCTG TGTCAGCTCA AGACACTTGC AAAGCACAAG
    TGTCATGTCA AACGTTTTGA CCACTACAAG TTTGAAGTGA CCATGGGGAT GCCCCAGAAG
    GGGCGCAATG GGGCACAAGA TTTTGATGAT GGCACAAAAG ACATTGTATT GCGGGCAAGC
    AATGAATTCC GGAGAGTCCT GCTGGTGCTC AGCTCAGGCA GTGTGGTGGA GTTCAGTACA
    GTTCTCGAGG GCAGACTGGG CAGCAAATGG CCAGTGTTTG AGCTTAAAGC CTTGAACTGC
    AAGACTTGTG CCTCACCTCT TATACCAATA CGACGACAGG TCAAGATCGA GCAGGATTGG
    AGGGTTAATG CCAGAAATGC CTTAGCATTT GCCTTCAATT TTGTTTTCCA TCCTCTACTT
    TCAGCTGAGA TGGATGCTAC TGCAGCTACT ACAGAAGTAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684510.2
    CDS191..2878
    Translation

    Target ORF information:

    RefSeq Version XM_679418.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679418.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACACGT CTCTGCTTGA ATCTCAGCTG TCGGCCCCAG CATCACCTGT TTCTCCAGCA 
    TTCTCTGTGT CTGGACACAA CATTCATCCA GTGTCCTCTT CTTCAACACC CAGCCCCGCT
    CCTTCAGCCA TGACTCCGCC CCCACATCAC GCCTCTCAGA TGGGTCGATC CCAGCTGAAC
    GCAGCCAGCA ATTCCAGCAC TGCCTTTCCA GGACAATCAG CGTTTCCTCC TCAGGGGGAA
    ATCAAAGAGG AGCTGAGCAT TGATGATCTT AAACAGAGGG CAAAGAATGG AGATGCTAAA
    GCACAGACAG AGATTGGTAG ATATTACCTG AGACTAGCCG AGCAAGAAGA TGAAGAGGTG
    AATGCTGTTA CTGCTGTAAC ATGGCTACTC CAGGCAGCCA AAAATGGACG AAAAGATGCA
    GTGAAGTTGC TACAGCGCTG CCTGCATGAC AGGAGGGGCA TCACAGCGGA GAACAGAGAG
    GAGGTGTGTT CCCTGGCATG TGAATCTCGT TTTCAGCGCA GCGTAAGGAA AGCAGCTCTG
    CTCATGTATT GGAAACTCAA TCCAGAACGC AAGAAAAACA TCACAGCCTC TGAACTTCTG
    GAAAATGTGA GCCAGCTCAA CACAGATGGC GCTTCCCGCA GCCCTCTCTC CAGCCCAATA
    CAGAAACAAA GAAAGATTTT GGAGAGTTTG GTGTCAAGAG ATGAGACTGA TTGCATTGGT
    GTAGAAGAGT TTGTTGAGAA TACCAAACAA TACGCTGAGG GTATTTCACC ATCCATGGAA
    GGGGTAGCAG GGGATGATGA TGACGATGAT GAAGAGCCAG TAAAACATCC AGATGAGCTG
    CCTTTGCACC AGAAGGTGTT GAAGTTTCCT CTTCATGCAG TGTTGGAGGT GAAAGAGGTG
    TTGATTGACT GGGCGTCGCG TGCAGGCATG CAGTGGATTA GCGCCCTTAT ACCTACTCAT
    CATGTCAATA CCCTCATCTT CTTCTTCATC ATTTCCAACC TCACCCTGGA GTTCTTTGTC
    CTCGTCATCC CCCTAATTAT ATTCTACCTC TCCTTTGTGT CAATGGTCAT CTGCACCCTA
    CGAGTCTTCC AGAACAGCAA GGCATGGGAG AACTTCAGAG CGTTGACCGT CATGTTGTCT
    CATTTTGAGC CGGGGATTGA CCTGGAGCAG GCCGAGTCTA ACTTCACATG GAATCACCTT
    GAGCCATACC TCTACTTTCT CCTTTCTACG CTTTTCCTTA TCTTTTCGTT TCCTGTGGCT
    GACAAGTCCT GGTTGCCATG TTCAGAACTG GCTATGGTTG CAGTCTTCTT CACTGTAAGC
    AGCTACTTGA GCCTGCGACC ATCGGCACAA CAGCATGCCA AATTTGCTCT GCTTTCCCAG
    GTTGCCTCCG CCATTTTTAC ATTCATTAAT CGGTTGCTGG GAGGCTGGGT GGGTCGTATA
    ATTGGCGGCG CTTGGTTCAG CATGCGCCTT GGTGACTGGT TGGTAATGCA TGTAGGTGTG
    CCTTGTGTTC TCTATCTCTA CCTACTGTTC TTGTGTACCC GTATGGCCAC AGCAGGTGGT
    GCACGTGGCA CCTACTGTGT GCTTCTTCCC AACCTAGTGT GCTTCATCTG GTGTGAAGTG
    TGTGTTACGC TGCTGCAAGA GTCCACTGTA TTGGGGCTAA TGCGCTCAGT TGTGGGCTAC
    CTACTTTTTT TCTTTGCTCT GCCCGTGCTG TCGCTGGCTC TAGCAGCTGT AATGCTGGTT
    CAGCTGGTGC AGTGGTTCCT CGCCCTGGAA CTGACCAAAA TGATCGTAAC AGTGTGCATC
    TGCGTGCTGC CTGTCCTGCT TCGCTGGTGG ACTCGTTTTA GCGTGTCGCC TTTGGCAGTG
    CTTCGCTCTT TGCGTCGTAG CAGTGTGGTG AAGTTGATCC TTGTGTGGAT TTCAGCGCTG
    GTGCTCTTCA GCTGGTTCTA CGTGTACCGT TCGGAAGGGA TGAAAGTATA CAACTCCACC
    CTGACCTGGC AGGAGTACAG TGACCTGTGC GGCCCTCGTG CCTGGAAGGA GCGTAACATG
    GCACATGCCC AGATCATCTG CAGCCACTTA GAGGGACACC GAGTGACGTG GGAGGGACGG
    TTCAAGTATG TTCGTGTCAC TGAGATTGAG AATGGGGCAC AAGCTGTCGT CAATCTTCTA
    CCAGTCTTGA TAGCAGACTG GGTCCGATGT CTGTATGGAG AGGAGTACCC TGCTTGTGAT
    GAGAATCAGA CGGGACCTGC TGAACCTCTG TGTCAGCTCA AGACACTTGC AAAGCACAAG
    TGTCATGTCA AACGTTTTGA CCACTACAAG TTTGAAGTGA CCATGGGGAT GCCCCAGAAG
    GGGCGCAATG GGGCACAAGA TTTTGATGAT GGCACAAAAG ACATTGTATT GCGGGCAAGC
    AATGAATTCC GGAGAGTCCT GCTGGTGCTC AGCTCAGGCA GTGTGGTGGA GTTCAGTACA
    GTTCTCGAGG GCAGACTGGG CAGCAAATGG CCAGTGTTTG AGCTTAAAGC CTTGAACTGC
    AAGACTTGTG CCTCACCTCT TATACCAATA CGACGACAGG TCAAGATCGA GCAGGATTGG
    AGGGTTAATG CCAGAAATGC CTTAGCATTT GCCTTCAATT TTGTTTTCCA TCCTCTACTT
    TCAGCTGAGA TGGATGCTAC TGCAGCTACT ACAGAAGTAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa19454
    Clone ID Related Accession (Same CDS sequence) XM_679418.6 , XM_679418.7
    Accession Version XM_679418.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product wolframin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679418.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACACGT CTCTGCTTGA ATCTCAGCTG TCGGCCCCAG CATCACCTGT TTCTCCAGCA 
    TTCTCTGTGT CTGGACACAA CATTCATCCA GTGTCCTCTT CTTCAACACC CAGCCCCGCT
    CCTTCAGCCA TGACTCCGCC CCCACATCAC GCCTCTCAGA TGGGTCGATC CCAGCTGAAC
    GCAGCCAGCA ATTCCAGCAC TGCCTTTCCA GGACAATCAG CGTTTCCTCC TCAGGGGGAA
    ATCAAAGAGG AGCTGAGCAT TGATGATCTT AAACAGAGGG CAAAGAATGG AGATGCTAAA
    GCACAGACAG AGATTGGTAG ATATTACCTG AGACTAGCCG AGCAAGAAGA TGAAGAGGTG
    AATGCTGTTA CTGCTGTAAC ATGGCTACTC CAGGCAGCCA AAAATGGACG AAAAGATGCA
    GTGAAGTTGC TACAGCGCTG CCTGCATGAC AGGAGGGGCA TCACAGCGGA GAACAGAGAG
    GAGGTGTGTT CCCTGGCATG TGAATCTCGT TTTCAGCGCA GCGTAAGGAA AGCAGCTCTG
    CTCATGTATT GGAAACTCAA TCCAGAACGC AAGAAAAACA TCACAGCCTC TGAACTTCTG
    GAAAATGTGA GCCAGCTCAA CACAGATGGC GCTTCCCGCA GCCCTCTCTC CAGCCCAATA
    CAGAAACAAA GAAAGATTTT GGAGAGTTTG GTGTCAAGAG ATGAGACTGA TTGCATTGGT
    GTAGAAGAGT TTGTTGAGAA TACCAAACAA TACGCTGAGG GTATTTCACC ATCCATGGAA
    GGGGTAGCAG GGGATGATGA TGACGATGAT GAAGAGCCAG TAAAACATCC AGATGAGCTG
    CCTTTGCACC AGAAGGTGTT GAAGTTTCCT CTTCATGCAG TGTTGGAGGT GAAAGAGGTG
    TTGATTGACT GGGCGTCGCG TGCAGGCATG CAGTGGATTA GCGCCCTTAT ACCTACTCAT
    CATGTCAATA CCCTCATCTT CTTCTTCATC ATTTCCAACC TCACCCTGGA GTTCTTTGTC
    CTCGTCATCC CCCTAATTAT ATTCTACCTC TCCTTTGTGT CAATGGTCAT CTGCACCCTA
    CGAGTCTTCC AGAACAGCAA GGCATGGGAG AACTTCAGAG CGTTGACCGT CATGTTGTCT
    CATTTTGAGC CGGGGATTGA CCTGGAGCAG GCCGAGTCTA ACTTCACATG GAATCACCTT
    GAGCCATACC TCTACTTTCT CCTTTCTACG CTTTTCCTTA TCTTTTCGTT TCCTGTGGCT
    GACAAGTCCT GGTTGCCATG TTCAGAACTG GCTATGGTTG CAGTCTTCTT CACTGTAAGC
    AGCTACTTGA GCCTGCGACC ATCGGCACAA CAGCATGCCA AATTTGCTCT GCTTTCCCAG
    GTTGCCTCCG CCATTTTTAC ATTCATTAAT CGGTTGCTGG GAGGCTGGGT GGGTCGTATA
    ATTGGCGGCG CTTGGTTCAG CATGCGCCTT GGTGACTGGT TGGTAATGCA TGTAGGTGTG
    CCTTGTGTTC TCTATCTCTA CCTACTGTTC TTGTGTACCC GTATGGCCAC AGCAGGTGGT
    GCACGTGGCA CCTACTGTGT GCTTCTTCCC AACCTAGTGT GCTTCATCTG GTGTGAAGTG
    TGTGTTACGC TGCTGCAAGA GTCCACTGTA TTGGGGCTAA TGCGCTCAGT TGTGGGCTAC
    CTACTTTTTT TCTTTGCTCT GCCCGTGCTG TCGCTGGCTC TAGCAGCTGT AATGCTGGTT
    CAGCTGGTGC AGTGGTTCCT CGCCCTGGAA CTGACCAAAA TGATCGTAAC AGTGTGCATC
    TGCGTGCTGC CTGTCCTGCT TCGCTGGTGG ACTCGTTTTA GCGTGTCGCC TTTGGCAGTG
    CTTCGCTCTT TGCGTCGTAG CAGTGTGGTG AAGTTGATCC TTGTGTGGAT TTCAGCGCTG
    GTGCTCTTCA GCTGGTTCTA CGTGTACCGT TCGGAAGGGA TGAAAGTATA CAACTCCACC
    CTGACCTGGC AGGAGTACAG TGACCTGTGC GGCCCTCGTG CCTGGAAGGA GCGTAACATG
    GCACATGCCC AGATCATCTG CAGCCACTTA GAGGGACACC GAGTGACGTG GGAGGGACGG
    TTCAAGTATG TTCGTGTCAC TGAGATTGAG AATGGGGCAC AAGCTGTCGT CAATCTTCTA
    CCAGTCTTGA TAGCAGACTG GGTCCGATGT CTGTATGGAG AGGAGTACCC TGCTTGTGAT
    GAGAATCAGA CGGGACCTGC TGAACCTCTG TGTCAGCTCA AGACACTTGC AAAGCACAAG
    TGTCATGTCA AACGTTTTGA CCACTACAAG TTTGAAGTGA CCATGGGGAT GCCCCAGAAG
    GGGCGCAATG GGGCACAAGA TTTTGATGAT GGCACAAAAG ACATTGTATT GCGGGCAAGC
    AATGAATTCC GGAGAGTCCT GCTGGTGCTC AGCTCAGGCA GTGTGGTGGA GTTCAGTACA
    GTTCTCGAGG GCAGACTGGG CAGCAAATGG CCAGTGTTTG AGCTTAAAGC CTTGAACTGC
    AAGACTTGTG CCTCACCTCT TATACCAATA CGACGACAGG TCAAGATCGA GCAGGATTGG
    AGGGTTAATG CCAGAAATGC CTTAGCATTT GCCTTCAATT TTGTTTTCCA TCCTCTACTT
    TCAGCTGAGA TGGATGCTAC TGCAGCTACT ACAGAAGTAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_684510.2
    CDS185..2872
    Translation

    Target ORF information:

    RefSeq Version XM_679418.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_679418.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACACGT CTCTGCTTGA ATCTCAGCTG TCGGCCCCAG CATCACCTGT TTCTCCAGCA 
    TTCTCTGTGT CTGGACACAA CATTCATCCA GTGTCCTCTT CTTCAACACC CAGCCCCGCT
    CCTTCAGCCA TGACTCCGCC CCCACATCAC GCCTCTCAGA TGGGTCGATC CCAGCTGAAC
    GCAGCCAGCA ATTCCAGCAC TGCCTTTCCA GGACAATCAG CGTTTCCTCC TCAGGGGGAA
    ATCAAAGAGG AGCTGAGCAT TGATGATCTT AAACAGAGGG CAAAGAATGG AGATGCTAAA
    GCACAGACAG AGATTGGTAG ATATTACCTG AGACTAGCCG AGCAAGAAGA TGAAGAGGTG
    AATGCTGTTA CTGCTGTAAC ATGGCTACTC CAGGCAGCCA AAAATGGACG AAAAGATGCA
    GTGAAGTTGC TACAGCGCTG CCTGCATGAC AGGAGGGGCA TCACAGCGGA GAACAGAGAG
    GAGGTGTGTT CCCTGGCATG TGAATCTCGT TTTCAGCGCA GCGTAAGGAA AGCAGCTCTG
    CTCATGTATT GGAAACTCAA TCCAGAACGC AAGAAAAACA TCACAGCCTC TGAACTTCTG
    GAAAATGTGA GCCAGCTCAA CACAGATGGC GCTTCCCGCA GCCCTCTCTC CAGCCCAATA
    CAGAAACAAA GAAAGATTTT GGAGAGTTTG GTGTCAAGAG ATGAGACTGA TTGCATTGGT
    GTAGAAGAGT TTGTTGAGAA TACCAAACAA TACGCTGAGG GTATTTCACC ATCCATGGAA
    GGGGTAGCAG GGGATGATGA TGACGATGAT GAAGAGCCAG TAAAACATCC AGATGAGCTG
    CCTTTGCACC AGAAGGTGTT GAAGTTTCCT CTTCATGCAG TGTTGGAGGT GAAAGAGGTG
    TTGATTGACT GGGCGTCGCG TGCAGGCATG CAGTGGATTA GCGCCCTTAT ACCTACTCAT
    CATGTCAATA CCCTCATCTT CTTCTTCATC ATTTCCAACC TCACCCTGGA GTTCTTTGTC
    CTCGTCATCC CCCTAATTAT ATTCTACCTC TCCTTTGTGT CAATGGTCAT CTGCACCCTA
    CGAGTCTTCC AGAACAGCAA GGCATGGGAG AACTTCAGAG CGTTGACCGT CATGTTGTCT
    CATTTTGAGC CGGGGATTGA CCTGGAGCAG GCCGAGTCTA ACTTCACATG GAATCACCTT
    GAGCCATACC TCTACTTTCT CCTTTCTACG CTTTTCCTTA TCTTTTCGTT TCCTGTGGCT
    GACAAGTCCT GGTTGCCATG TTCAGAACTG GCTATGGTTG CAGTCTTCTT CACTGTAAGC
    AGCTACTTGA GCCTGCGACC ATCGGCACAA CAGCATGCCA AATTTGCTCT GCTTTCCCAG
    GTTGCCTCCG CCATTTTTAC ATTCATTAAT CGGTTGCTGG GAGGCTGGGT GGGTCGTATA
    ATTGGCGGCG CTTGGTTCAG CATGCGCCTT GGTGACTGGT TGGTAATGCA TGTAGGTGTG
    CCTTGTGTTC TCTATCTCTA CCTACTGTTC TTGTGTACCC GTATGGCCAC AGCAGGTGGT
    GCACGTGGCA CCTACTGTGT GCTTCTTCCC AACCTAGTGT GCTTCATCTG GTGTGAAGTG
    TGTGTTACGC TGCTGCAAGA GTCCACTGTA TTGGGGCTAA TGCGCTCAGT TGTGGGCTAC
    CTACTTTTTT TCTTTGCTCT GCCCGTGCTG TCGCTGGCTC TAGCAGCTGT AATGCTGGTT
    CAGCTGGTGC AGTGGTTCCT CGCCCTGGAA CTGACCAAAA TGATCGTAAC AGTGTGCATC
    TGCGTGCTGC CTGTCCTGCT TCGCTGGTGG ACTCGTTTTA GCGTGTCGCC TTTGGCAGTG
    CTTCGCTCTT TGCGTCGTAG CAGTGTGGTG AAGTTGATCC TTGTGTGGAT TTCAGCGCTG
    GTGCTCTTCA GCTGGTTCTA CGTGTACCGT TCGGAAGGGA TGAAAGTATA CAACTCCACC
    CTGACCTGGC AGGAGTACAG TGACCTGTGC GGCCCTCGTG CCTGGAAGGA GCGTAACATG
    GCACATGCCC AGATCATCTG CAGCCACTTA GAGGGACACC GAGTGACGTG GGAGGGACGG
    TTCAAGTATG TTCGTGTCAC TGAGATTGAG AATGGGGCAC AAGCTGTCGT CAATCTTCTA
    CCAGTCTTGA TAGCAGACTG GGTCCGATGT CTGTATGGAG AGGAGTACCC TGCTTGTGAT
    GAGAATCAGA CGGGACCTGC TGAACCTCTG TGTCAGCTCA AGACACTTGC AAAGCACAAG
    TGTCATGTCA AACGTTTTGA CCACTACAAG TTTGAAGTGA CCATGGGGAT GCCCCAGAAG
    GGGCGCAATG GGGCACAAGA TTTTGATGAT GGCACAAAAG ACATTGTATT GCGGGCAAGC
    AATGAATTCC GGAGAGTCCT GCTGGTGCTC AGCTCAGGCA GTGTGGTGGA GTTCAGTACA
    GTTCTCGAGG GCAGACTGGG CAGCAAATGG CCAGTGTTTG AGCTTAAAGC CTTGAACTGC
    AAGACTTGTG CCTCACCTCT TATACCAATA CGACGACAGG TCAAGATCGA GCAGGATTGG
    AGGGTTAATG CCAGAAATGC CTTAGCATTT GCCTTCAATT TTGTTTTCCA TCCTCTACTT
    TCAGCTGAGA TGGATGCTAC TGCAGCTACT ACAGAAGTAT CTGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148810
    Clone ID Related Accession (Same CDS sequence) XM_021481141.1
    Accession Version XM_021481141.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product wolframin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTATTGGA AACTCAATCC AGAACGCAAG AAAAACATCA CAGCCTCTGA ACTTCTGGAA 
    AATGTGAGCC AGCTCAACAC AGATGGCGCT TCCCGCAGCC CTCTCTCCAG CCCAATACAG
    AAACAAAGAA AGATTTTGGA GAGTTTGGTG TCAAGAGATG AGACTGATTG CATTGGTGTA
    GAAGAGTTTG TTGAGAATAC CAAACAATAC GCTGAGGGTA TTTCACCATC CATGGAAGGG
    GTAGCAGGGG ATGATGATGA CGATGATGAA GAGCCAGTAA AACATCCAGA TGAGCTGCCT
    TTGCACCAGA AGGTGTTGAA GTTTCCTCTT CATGCAGTGT TGGAGGTGAA AGAGGTGTTG
    ATTGACTGGG CGTCGCGTGC AGGCATGCAG TGGATTAGCG CCCTTATACC TACTCATCAT
    GTCAATACCC TCATCTTCTT CTTCATCATT TCCAACCTCA CCCTGGAGTT CTTTGTCCTC
    GTCATCCCCC TAATTATATT CTACCTCTCC TTTGTGTCAA TGGTCATCTG CACCCTACGA
    GTCTTCCAGA ACAGCAAGGC ATGGGAGAAC TTCAGAGCGT TGACCGTCAT GTTGTCTCAT
    TTTGAGCCGG GGATTGACCT GGAGCAGGCC GAGTCTAACT TCACATGGAA TCACCTTGAG
    CCATACCTCT ACTTTCTCCT TTCTACGCTT TTCCTTATCT TTTCGTTTCC TGTGGCTGAC
    AAGTCCTGGT TGCCATGTTC AGAACTGGCT ATGGTTGCAG TCTTCTTCAC TGTAAGCAGC
    TACTTGAGCC TGCGACCATC GGCACAACAG CATGCCAAAT TTGCTCTGCT TTCCCAGGTT
    GCCTCCGCCA TTTTTACATT CATTAATCGG TTGCTGGGAG GCTGGGTGGG TCGTATAATT
    GGCGGCGCTT GGTTCAGCAT GCGCCTTGGT GACTGGTTGG TAATGCATGT AGGTGTGCCT
    TGTGTTCTCT ATCTCTACCT ACTGTTCTTG TGTACCCGTA TGGCCACAGC AGGTGGTGCA
    CGTGGCACCT ACTGTGTGCT TCTTCCCAAC CTAGTGTGCT TCATCTGGTG TGAAGTGTGT
    GTTACGCTGC TGCAAGAGTC CACTGTATTG GGGCTAATGC GCTCAGTTGT GGGCTACCTA
    CTTTTTTTCT TTGCTCTGCC CGTGCTGTCG CTGGCTCTAG CAGCTGTAAT GCTGGTTCAG
    CTGGTGCAGT GGTTCCTCGC CCTGGAACTG ACCAAAATGA TCGTAACAGT GTGCATCTGC
    GTGCTGCCTG TCCTGCTTCG CTGGTGGACT CGTTTTAGCG TGTCGCCTTT GGCAGTGCTT
    CGCTCTTTGC GTCGTAGCAG TGTGGTGAAG TTGATCCTTG TGTGGATTTC AGCGCTGGTG
    CTCTTCAGCT GGTTCTACGT GTACCGTTCG GAAGGGATGA AAGTATACAA CTCCACCCTG
    ACCTGGCAGG AGTACAGTGA CCTGTGCGGC CCTCGTGCCT GGAAGGAGCG TAACATGGCA
    CATGCCCAGA TCATCTGCAG CCACTTAGAG GGACACCGAG TGACGTGGGA GGGACGGTTC
    AAGTATGTTC GTGTCACTGA GATTGAGAAT GGGGCACAAG CTGTCGTCAA TCTTCTACCA
    GTCTTGATAG CAGACTGGGT CCGATGTCTG TATGGAGAGG AGTACCCTGC TTGTGATGAG
    AATCAGACGG GACCTGCTGA ACCTCTGTGT CAGCTCAAGA CACTTGCAAA GCACAAGTGT
    CATGTCAAAC GTTTTGACCA CTACAAGTTT GAAGTGACCA TGGGGATGCC CCAGAAGGGG
    CGCAATGGGG CACAAGATTT TGATGATGGC ACAAAAGACA TTGTATTGCG GGCAAGCAAT
    GAATTCCGGA GAGTCCTGCT GGTGCTCAGC TCAGGCAGTG TGGTGGAGTT CAGTACAGTT
    CTCGAGGGCA GACTGGGCAG CAAATGGCCA GTGTTTGAGC TTAAAGCCTT GAACTGCAAG
    ACTTGTGCCT CACCTCTTAT ACCAATACGA CGACAGGTCA AGATCGAGCA GGATTGGAGG
    GTTAATGCCA GAAATGCCTT AGCATTTGCC TTCAATTTTG TTTTCCATCC TCTACTTTCA
    GCTGAGATGG ATGCTACTGC AGCTACTACA GAAGTATCTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336816.1
    CDS164..2308
    Translation

    Target ORF information:

    RefSeq Version XM_021481141.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Wolfram syndrome 1b (wolframin) (wfs1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021481141.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTATTGGA AACTCAATCC AGAACGCAAG AAAAACATCA CAGCCTCTGA ACTTCTGGAA 
    AATGTGAGCC AGCTCAACAC AGATGGCGCT TCCCGCAGCC CTCTCTCCAG CCCAATACAG
    AAACAAAGAA AGATTTTGGA GAGTTTGGTG TCAAGAGATG AGACTGATTG CATTGGTGTA
    GAAGAGTTTG TTGAGAATAC CAAACAATAC GCTGAGGGTA TTTCACCATC CATGGAAGGG
    GTAGCAGGGG ATGATGATGA CGATGATGAA GAGCCAGTAA AACATCCAGA TGAGCTGCCT
    TTGCACCAGA AGGTGTTGAA GTTTCCTCTT CATGCAGTGT TGGAGGTGAA AGAGGTGTTG
    ATTGACTGGG CGTCGCGTGC AGGCATGCAG TGGATTAGCG CCCTTATACC TACTCATCAT
    GTCAATACCC TCATCTTCTT CTTCATCATT TCCAACCTCA CCCTGGAGTT CTTTGTCCTC
    GTCATCCCCC TAATTATATT CTACCTCTCC TTTGTGTCAA TGGTCATCTG CACCCTACGA
    GTCTTCCAGA ACAGCAAGGC ATGGGAGAAC TTCAGAGCGT TGACCGTCAT GTTGTCTCAT
    TTTGAGCCGG GGATTGACCT GGAGCAGGCC GAGTCTAACT TCACATGGAA TCACCTTGAG
    CCATACCTCT ACTTTCTCCT TTCTACGCTT TTCCTTATCT TTTCGTTTCC TGTGGCTGAC
    AAGTCCTGGT TGCCATGTTC AGAACTGGCT ATGGTTGCAG TCTTCTTCAC TGTAAGCAGC
    TACTTGAGCC TGCGACCATC GGCACAACAG CATGCCAAAT TTGCTCTGCT TTCCCAGGTT
    GCCTCCGCCA TTTTTACATT CATTAATCGG TTGCTGGGAG GCTGGGTGGG TCGTATAATT
    GGCGGCGCTT GGTTCAGCAT GCGCCTTGGT GACTGGTTGG TAATGCATGT AGGTGTGCCT
    TGTGTTCTCT ATCTCTACCT ACTGTTCTTG TGTACCCGTA TGGCCACAGC AGGTGGTGCA
    CGTGGCACCT ACTGTGTGCT TCTTCCCAAC CTAGTGTGCT TCATCTGGTG TGAAGTGTGT
    GTTACGCTGC TGCAAGAGTC CACTGTATTG GGGCTAATGC GCTCAGTTGT GGGCTACCTA
    CTTTTTTTCT TTGCTCTGCC CGTGCTGTCG CTGGCTCTAG CAGCTGTAAT GCTGGTTCAG
    CTGGTGCAGT GGTTCCTCGC CCTGGAACTG ACCAAAATGA TCGTAACAGT GTGCATCTGC
    GTGCTGCCTG TCCTGCTTCG CTGGTGGACT CGTTTTAGCG TGTCGCCTTT GGCAGTGCTT
    CGCTCTTTGC GTCGTAGCAG TGTGGTGAAG TTGATCCTTG TGTGGATTTC AGCGCTGGTG
    CTCTTCAGCT GGTTCTACGT GTACCGTTCG GAAGGGATGA AAGTATACAA CTCCACCCTG
    ACCTGGCAGG AGTACAGTGA CCTGTGCGGC CCTCGTGCCT GGAAGGAGCG TAACATGGCA
    CATGCCCAGA TCATCTGCAG CCACTTAGAG GGACACCGAG TGACGTGGGA GGGACGGTTC
    AAGTATGTTC GTGTCACTGA GATTGAGAAT GGGGCACAAG CTGTCGTCAA TCTTCTACCA
    GTCTTGATAG CAGACTGGGT CCGATGTCTG TATGGAGAGG AGTACCCTGC TTGTGATGAG
    AATCAGACGG GACCTGCTGA ACCTCTGTGT CAGCTCAAGA CACTTGCAAA GCACAAGTGT
    CATGTCAAAC GTTTTGACCA CTACAAGTTT GAAGTGACCA TGGGGATGCC CCAGAAGGGG
    CGCAATGGGG CACAAGATTT TGATGATGGC ACAAAAGACA TTGTATTGCG GGCAAGCAAT
    GAATTCCGGA GAGTCCTGCT GGTGCTCAGC TCAGGCAGTG TGGTGGAGTT CAGTACAGTT
    CTCGAGGGCA GACTGGGCAG CAAATGGCCA GTGTTTGAGC TTAAAGCCTT GAACTGCAAG
    ACTTGTGCCT CACCTCTTAT ACCAATACGA CGACAGGTCA AGATCGAGCA GGATTGGAGG
    GTTAATGCCA GAAATGCCTT AGCATTTGCC TTCAATTTTG TTTTCCATCC TCTACTTTCA
    GCTGAGATGG ATGCTACTGC AGCTACTACA GAAGTATCTG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.